| psychoactive mushroom use in koh samui and koh pha-ngan, thailand. | this paper presents the results of recent ethnomycological exploration in southern thailand. field observations, interviews and collection of fungi specimens were carried out primarily on two islands, koh samui and koh pha-ngan, situated in the western region of the gulf of siam. some fieldwork was also conducted in the northern thai province of chiang dao and in the southern thai province of surat thani. during five separate excursions (1989-90), observations were made of occurrence, harvesting ... | 1992 | 1548895 |
| phylogeny and 16s rrna sequence of magnetospirillum sp. amb-1, an aerobic magnetic bacterium. | | 1992 | 1549478 |
| differential sensitivity to fibrinogen inhibition of sp-c- vs. sp-b-based surfactants. | inhibition of surfactant function by plasma-derived proteins is assumed to occur under conditions of alveolar protein leakage. we investigated surface properties and sensitivity to the inhibitory capacity of fibrinogen (fbg) of artificial surfactants in a pulsating-bubble surfactometer. phospholipid-fatty acid mixtures (plm) with or without hydrophobic apoproteins [natural bovine surfactant proteins (sp) b and c, recombinant human sp-c] were used. without fbg, all apoprotein-based surfactants ex ... | 1992 | 1550251 |
| mixtures of synthetic peptides and dipalmitoylphosphatidylcholine as lung surfactants. | synthetic peptides that differ in their lipid-peptide interactions were combined with dipalmitoylphosphatidylcholine (dppc) and tested in an adult rat lavaged lung model in vitro for efficacy as totally synthetic lung surfactants. the putative amphipathic alpha-helical region of the major lung surfactant apoprotein (sp-a81-102), an analogue with increased amphipathic alpha-helical potential ([lys88,97,glu99,trp102]-sp-a81-102]), and the hydrophobic peptide gramicidin d were all ineffective. thre ... | 1992 | 1550252 |
| effects of nifedipine and moxonidine on cardiac structure in spontaneously hypertensive rats. stereological studies on myocytes, capillaries, arteries, and cardiac interstitium. | light and electron microscopic stereological studies were performed on the myocardium of spontaneously hypertensive rats (shr-sp) before and after treatment with nifedipine (27 mg/kg body weight/day) and the antisympathotonic agent moxonidine (8 mg/kg body weight/day). the treated groups were compared with nontreated shr-sp and normotensive wky (n = 10 in each group). at the beginning of therapy (when the male shr-sp were 6 months old), blood pressure was increased and left ventricular hypertrop ... | 1992 | 1550668 |
| three c-phycoerythrin-associated linker polypeptides in the phycobilisome of green-light-grown calothrix sp. pcc 7601 (cyanobacteria). | microanalyses by sds-page and microsequencing demonstrate that, under green-light conditions, 3 c-phycoerythrin associated rod-linker polypeptides with different n-terminal amino acid sequences are present in phycobilisomes (pbs) from calothrix sp. 7601 cells. two of these polypeptides, corresponding to sds-page bands at 36 and 37 kda, could be assigned, respectively, to the cpec and cpcd genes found on a separate cpecd-operon in calothrix sp. 7601 (federspiel, n.a. and grossman, a.r. (1990) j. ... | 1992 | 1551428 |
| sp-40,40, a protein involved in the control of the complement pathway, possesses a unique array of disulphide bridges. | sp-40,40 is a two-chain serum protein which acts in vitro as a potent inhibitor of the assembly of the membrane attack complex of human complement. it contains 10 cysteine residues, the numbers and locations of which are conserved in several mammalian species. evidence is presented that all the cysteine residues are involved in interchain (alpha-beta) disulphide bonds. there are no free cysteine residues. the disulphide bond motif established in this study for sp-40,40 is unique and bears no obv ... | 1992 | 1551440 |
| both the anaerobic pathway and aerobic desaturation are involved in the synthesis of unsaturated fatty acids in vibrio sp. strain abe-1. | vibrio sp. strain abe-1 is a unique marine bacterium in terms of its ability to synthesize delta 9-trans-hexadecenoic acid and delta 7-cis-tetradecenoic acid (14:1(7c); okuyama, h., sasaki, s., higashi, s. and murata, n. (1990) j. bacteriol. 172, 3515-3518). the present study, involving labeling with [1-14c]acetate, demonstrated that 14:1 is synthesized by the anaerobic pathway. when cells of this bacterium were grown in the presence of [1-14c]myristic acid (14:0), this compound was converted to ... | 1992 | 1551444 |
| the psac genes of synechococcus sp. pcc7002 and cyanophora paradoxa: cloning and sequence analysis. | the psac genes of the cyanobacterium, synechococcus sp. pcc7002, and of the cyanelle genome of the phylogenetically ambiguous biflagellate, cyanophora paradoxa, were cloned, mapped and sequenced. the psac proteins of both species exhibit high degrees (approx. 95%) of sequence similarity to the psac proteins of other cyanobacteria as well as the chloroplast-encoded proteins of green algae and higher plants. the synechococcus sp. pcc7002 psac gene is transcribed as a monocistronic mrna of approx. ... | 1992 | 1551590 |
| the bile acid-inducible baib gene from eubacterium sp. strain vpi 12708 encodes a bile acid-coenzyme a ligase. | the baib gene from eubacterium sp. strain vpi 12708 was previously cloned, sequenced, and shown to be part of a large bile acid-inducible operon encoding polypeptides believed to be involved in bile acid 7 alpha-dehydroxylation. in the present study, the baib gene was subcloned and expressed in escherichia coli and shown to encode a bile acid-coenzyme a (coa) ligase. this ligase required a c-24 bile acid with a free carboxyl group, atp, mg2+, and coa for synthesis of the final bile acid-coa conj ... | 1992 | 1551828 |
| structural and biochemical characterization of the escherichia coli arge gene product. | the dna sequence of a 2,100-bp region containing the arge gene from escherichia coli has been determined. the nucleotide sequence of the ppc-arge intergenic region was also solved and shown to contain six tandemly repeated rep sequences. moreover, the oxyr gene has been mapped on the e. coli chromosome and shown to flank the arg operon. the codon responsible for the translation start of arge was determined by using site-directed mutants. this gene spans 1,400 bp and encodes a 42,350-da polypepti ... | 1992 | 1551850 |
| three new species of laelaps (acari: laelapidae) associated with small mammals in central brazil. | three new species of laelaps koch are described and illustrated from small mammals in the federal district of brazil. each new species is primarily associated with one species of rodent: l. valdevinoi n. sp. from calomys tener (winge); l. fonsecai n. sp. from rhipidomys mastacalis (lund); and l. furmani n. sp. from oryzomys bicolor (tomes). two of the new species were previously included under names species, from which they are now separated: l. fonsecai n. sp. from l. paulistanensis fonseca, 19 ... | 1992 | 1552531 |
| argas (argas) monolakensis, new species (acari: ixodoidea: argasidae), a parasite of california gulls on islands in mono lake, california: description, biology, and life cycle. | argas (argas) monolakensis, n. sp., is described from adults, nymphs, and larvae collected from under and around nests of california gulls, larus californicus lawrence, on islands in mono lake, mono county, calif., and from specimens reared in the laboratory. this species is closely related to a. cooleyi kohls & hoogstraal, a parasite of cliff swallows, hirundo pyrrhonota vieillot, but is easily distinguished by hypostome dentition and roof of haller's organ in all stages and chaetotaxy of the l ... | 1992 | 1552533 |
| dna in dormant spores of bacillus species is in an a-like conformation. | the dna in dormant spores of bacillus species is associated with alpha/beta-type small, acid-soluble proteins (sasp), which are double-stranded dna-binding proteins whose amino acid sequence has been highly conserved in evolution. in vitro these proteins bind most strongly to dna which readily adopts an a-like conformation, as binding of alpha/beta-type sasp causes dna to assume an a-like conformation. as predicted by this conformational change in dna, binding of alpha/beta-type sasp to relaxed ... | 1992 | 1552855 |
| identification of replication and stability functions in the complete nucleotide sequence of plasmid puh24 from the cyanobacterium synechococcus sp. pcc 7942. | the complete nucleotide sequence is presented for puh24, the small plasmid of synechococcus sp. pcc 7942. puh24 consists of 7835bp and has a g + c content of 59%. the distribution of translation start and stop codons in the sequence allows 36 open reading frames that potentially encode polypeptides of 50 or more amino acids. we postulate that eight of these open reading frames are actual coding sequences. a region has been identified, by experiment, that contains two functions, designated pmaa a ... | 1992 | 1552863 |
| [the pathogenesis of the "endometritis-pyometra complex" in the female dog]. | based on investigations in 51 bitches (uteri and ovaries after ovariohysterectomy), suffering from "endometritis-pyometra-complex" (without, n = 38; with, n = 13, hormonal pretreatment), morphologic-functional endometrial characteristics (light microscopy, scanning electron microscopy, enzyme histochemistry, immunohistochemical estrogen receptor analysis) are correlated with ovarian findings, plasma estradiol and progesterone levels as well as microbiological results. the estrous phase of all cy ... | 1992 | 1553839 |
| tympanic electrocochleography: normal and abnormal patterns of response. | electrocochleography has been widely used in human and animal studies of endolymphatic hydrops. a variety of response patterns have been reported in normal and hydropic ears. recent clinical studies have focused almost exclusively on the amplitude ratio of the summating potential (sp) and action potential (ap) derived from alternating polarity click responses. in this report normal response patterns are described with a tympanic membrane electrode employing condensation, rarefaction and alternat ... | 1992 | 1554333 |
| bacterial metabolism of 5-aminosalicylic acid. initial ring cleavage. | the metabolism of 5-aminosalicylate (5as) by a bacterial strain, pseudomonas sp. bn9, was studied. intact cells of pseudomonas sp. bn9 grown with 5as oxidized 5as and 2,5-dihydroxybenzoate (gentisate), whereas cells grown with gentisate oxidized only the growth substrate of all substituted salicylates tested. cell extracts from pseudomonas sp. bn9 catalysed the stoichiometric reaction of 1 mol of oxygen with 1 mol of 5as to a metabolite with an intense u.v.-absorption maximum at 352 nm (ph 8.0). ... | 1992 | 1554350 |
| multiple molecular alterations in mouse lung tumors. | twenty-five mouse lung tumors induced by a single urethan treatment in female a/j, balb/c, and (a/j x c3h/he)f1 (ac3) mice were analyzed for the presence of mutations at codon 61 of the ki-ras gene and for the expression of the surfactant protein a (sp-a), retinoblastoma (rb), growth arrest-specific-3 (gas-3), p53, c-myc, and thymidylate synthase (ts) genes. ki-ras codon 61 mutations were detected in 22 of 25 tumor samples without differences among strains. in comparison with normal lungs, all t ... | 1992 | 1554414 |
| electrocochleographic recordings in acute and healed perilymphatic fistula. | in this study, the summating potential (sp) and action potential (ap) amplitude ratio was measured in 15 guinea pigs before and after surgical induction of a round window fistula. the round window fistula was then allowed to heal, and recordings were obtained after 1 month. stimuli included 8000-hz and 2000-hz tone bursts and click stimuli at levels ranging from 100-db peak equivalent sound pressure level to ap threshold. immediately after fistula induction, a statistically significant enlargeme ... | 1992 | 1554452 |
| molecular characterization of ferredoxin-nadp+ oxidoreductase in cyanobacteria: cloning and sequence of the peth gene of synechococcus sp. pcc 7002 and studies on the gene product. | the peth gene encoding ferredoxin-nadp+ oxidoreductase (fnr) was cloned and sequenced from the cyanobacterium synechococcus sp. pcc 7002. the deduced amino acid sequence of the fnr protein (402 amino acids) is approximately 110 amino acids longer at the n-terminus than fnr proteins which have been characterized from other cyanobacteria. n-terminal amino acid sequence analysis of the protein confirms the assigned translational start codon and shows that the initiator methionine is not removed. ma ... | 1992 | 1554697 |
| vascular, ganglia and cardiac catecholamine disposition in the spontaneously hypertensive rat and in the stroke-prone spontaneously hypertensive rat. | we have examined the disposition of catecholamines in cardiac tissue, mesenteric arteries and ganglia from normotensive wistar-kyoto rats (wky), spontaneously hypertensive rats (shr) and stroke-prone spontaneously hypertensive rats (shr-sp). the norepinephrine (ne) contents of mesenteric arteries from all three strains of rats adhered to a pattern which was characterized by the largest concentrations of the catecholamine in arteries from shr and shr-sp rats, and the smallest values present in me ... | 1992 | 1554865 |
| a new series of natural antifungals that inhibit p450 lanosterol c-14 demethylase. i. taxonomy, fermentation, isolation and structural elucidation. | a new series of antifungal antibiotics, ro 09-1470 and its 6 congeners were isolated from the fermentation broth of penicillium sp. nr6564. their structures were determined as tetrahydropyran derivatives with an alkenyl side chain on the basis of their spectroscopic and physico-chemical properties. among these compounds, ro 09-1470 and ro 09-1545 possessing a glycyl n-substituted glycyl ester residue had high antifungal activity. ro 09-1469, one of the congeners, was found in the fermentation br ... | 1992 | 1556006 |
| a new series of natural antifungals that inhibit p450 lanosterol c-14 demethylase. ii. mode of action. | from a penicillium sp. we identified a new series of antifungals having a tetrahydropyran skeleton with an alkenyl side chain. we elucidated the mode of action of ro 09-1470, the most active compound of the series. treatment of candida albicans with the compound caused an accumulation of c-14 methyl intermediates of ergosterol at concentrations of which no significant interference with the biosyntheses of other macromolecules and respiration was observed. p450 lanosterol c-14 demethylase (p450(1 ... | 1992 | 1556007 |
| new cholesterol biosynthesis inhibitors mc-031 (o-demethylchlorothricin), -032 (o-demethylhydroxychlorothricin), -033 and -034. | in the course of our screening for microbial compounds which possessed inhibitory activities against biosynthesis of cholesterol from mevalonate, we isolated four new compounds from the cultural broth of streptomyces sp. a7361. they are structurally related to, but distinct from chlorothricin. | 1992 | 1556012 |
| proline-specific endopeptidases from microbial sources: isolation of an enzyme from a xanthomonas sp. | an extensive screening among microorganisms for the presence of post-proline-specific endopeptidase activity was performed. this activity was found among ordinary bacteria from soil samples but not among fungi and actinomycetes. this result is in contrast to the previous notion that this activity is confined to the genus flavobacterium. a proline endopeptidase was isolated from a xanthomonas sp. and characterized with respect to physicochemical and enzymatic properties. the enzyme is composed of ... | 1992 | 1556065 |
| activities of two dissimilar thioredoxins from the cyanobacterium anabaena sp. strain pcc 7120. | thioredoxin is a small redox protein that functions as a reducing agent and modulator of enzyme activity. a gene for an unusual thioredoxin was previously isolated from the cyanobacterium anabaena sp. strain pcc 7120 and cloned and expressed in escherichia coli. however, the protein could not be detected in anabaena cells (j. alam, s. curtis, f. k. gleason, m. gerami-nejad, and j. a. fuchs, j. bacteriol. 171:162-171, 1989). polyclonal antibodies to the atypical thioredoxin were prepared, and the ... | 1992 | 1556078 |
| cloning and partial sequencing of an operon encoding two pseudomonas putida haloalkanoate dehalogenases of opposite stereospecificity. | we have cloned fragments of dna (up to 13 kb), from pseudomonas putida aj1, that code for two stereospecific haloalkanoate dehalogenases. these enzymes are highly specific for d and l substrates. the two genes, designated hadd and hadl, have been isolated and independently expressed in escherichia coli and p. putida hosts by using broad-host-range vectors. they are closely adjacent and inducible in what appears to be an operon with an upstream open reading frame of unknown function. nucleotide s ... | 1992 | 1556080 |
| genes encoding the phycobilisome rod substructure are clustered on the anabaena chromosome: characterization of the phycoerythrocyanin operon. | the phycoerythrocyanin (pec) operon, cloned from anabaena sp. strain pcc 7120, encodes four genes, pecbace, located upstream of the c-phycocyanin (cpc) operon. this pec-cpc cluster includes all the genes for the structural components of the phycobilisome rod. oligonucleotide probes based on the amino-terminal sequence of the phycoerythrocyanin beta subunit were used to clone an 8.0-kbp ecori fragment which was determined, by sequencing, to partially overlap the previously cloned cpc operon. a 5. ... | 1992 | 1556083 |
| construction of an insertional-inactivation cloning vector for escherichia coli using a rhodococcus gene for indigo production. | pslh8, an insertional-inactivation cloning vector for escherichia coli has been constructed by inserting a pigment gene (probably encoding an indole dioxygenase) from rhodococcus sp. atcc 21145 into puc18. wild-type e. coli colonies containing pslh8 produce insoluble indigo and turn dark blue on unsupplemented lb agar. insertion of dna fragments into the unique bamhi, ecori, ecorv, hindiii, psti, sphi and ssti polylinker cloning sites disrupts the reading frame of the fully sequenced pigment gen ... | 1992 | 1556550 |
| self cloning in micromonospora olivasterospora of fms genes for fortimicin a (astromicin) biosynthesis. | we have cloned the seven genes that are responsible for biosynthesis of the antibiotic fortimicin a (ftm a) using a recently developed self-cloning system that employes the plasmid vector pmo116 for micromonospora olivasterospora. five chimeric plasmids that restored ftm a production in m. olivasterospora mutants blocked at different biosynthetic steps were isolated by shotgun cloning. secondary transformation using other non-producing mutants showed that two additional ftm a biosynthetic genes ... | 1992 | 1557033 |
| visual identification of bacterially contaminated red cells. | there have been increasing numbers of reports of transfusion-acquired yersinia enterocolitica bacteremia (including several fatal cases). fifteen units of whole blood were inoculated with various concentrations of y. enterocolitica serotype 0:3 and processed into as-3 preserved red cells (rbcs). consistent growth of the organism was found at inoculum concentrations greater than or equal to 10 colony-forming units per ml. in all 13 units of rbcs that supported the growth of y. enterocolitica, a d ... | 1992 | 1557802 |
| [distribution of 70 kd stress protein in the optic nerve. a comparative study between the normal and retinal dystrophic rat]. | the immunolocalization of the 70 kd stress protein (sp70) was investigated and compared in the optic nerve of normal sprague-dawley (sd) rats and that of the royal college of surgeons (rcs) rat with inherited retinal dystrophy. at postnatal day 8, sp70 was present in the maturing glial cell bodies of both rat strains. at postnatal day 22, sp 70 was observed in the glial cell bodies and optic nerve fibers of both rat strains. at postnatal day 40 rcs rat, sp70 was diminished in the optic nerve fib ... | 1992 | 1558016 |
| seroepidemiologic study of toxocariasis and strongyloidiasis in institutionalized mentally retarded adults. | serologic surveys for toxocara canis and strongyloides sp., as well as stool examinations for intestinal parasites, were conducted in a home for mentally retarded adults. evidence of parasitic infection was found in 30 (28.3%) of 106 residents; nine (8.5%) had positive toxocaral serology (enzyme-linked immunosorbent assay[elisa]), 1 (0.9%) had positive serology for stronglyoides sp. (elisa), and 21 (19.8%) had parasites in stool (including stronglyoides stercoralis in the patient with positive s ... | 1992 | 1558266 |
| possible determination of the vector and reservoir of leishmaniasis in the dominican republic. | two species of sand flies were collected by various methods from sites in the dominican republic. lutzomyia cayennensis hispaniolae was the more common of the two. it was found in wooded habitats from sea level to an elevation of 442 m. this species was observed feeding on lizards (anolis sp.) in the wild. in the laboratory, it fed only on lizards and only under lighted conditions. the other species, lu. christophei was only found in the vicinity of seven leishmaniasis case sites. it readily fed ... | 1992 | 1558267 |
| penetration in vitro of newly excysted juvenile flukes of japanese fasciola sp. through ligated intestines of rabbits, mice, rats and chickens. | ligated intestines of rabbits, mice, rats and chickens were used to examine the penetration of newly excysted juvenile flukes of japanese fasciola sp. in vitro. in rabbit intestines, the penetration rate was relatively high in the rectum and duodenum. penetration rates in the jejunum, ileum, cecum and colon were comparable to those in the rectum and duodenum, although it was lower in the appendix. in the case of mouse, juvenile flukes penetrated the duodenum, jejunum, cecum, and rectum at consid ... | 1992 | 1558891 |
| factors contributing to the resistance of chickens to infection with japanese fasciola sp. | attempts were made to clarify the factors contributing to the resistance of chickens to infection with japanese fasciola sp. infection was not successfully established in chickens by oral inoculation of metacercariae, nor by inoculation of excysted juvenile flukes into the body cavity or to the liver surface. many metacercarial cysts were detected within two days in the feces of orally inoculated chickens. in the in vitro excystation test with chicken bile at 42 degrees c, metacercariae emerged ... | 1992 | 1558893 |
| nucleotide sequence and expression of the gene for the 9 kda fa/fb component of photosystem i from the cyanobacterium anabaena sp. pcc7120. | the nucleotide sequence of an 813 bp ssp i-hinc ii fragment containing the psac gene from anabaena sp. pcc 7120 is reported. the gene encodes a polypeptide of 81 amino acids, has a single transcript of size approximately 480 nucleotides and a single startpoint of transcription. it is flanked by a ribosome binding site on the 5' end and a potential transcription terminator on the 3' end. comparison of the amino acid sequences of the psac gene product from sixteen organisms shows that cyanobacteri ... | 1992 | 1558954 |
| isolation and identification of two neutral thyrotropin releasing hormone-like peptides, pyroglutamylphenylalanineproline amide and pyroglutamylglutamineproline amide, from human seminal fluid. | two tripeptide amides with stuctures similar to thyrotropin releasing hormone were isolated from human seminal fluid and their amino acid sequences determined. the peptides were purified by gel exclusion from sephadex g50 and were detected by radioimmunoassay with thyrotropin releasing hormone antibody; in addition, n-terminally extended forms were demonstrated by radioimmunoassay after trypsin digestion. further purification of the tripeptides was by chromatography on sp-sephadex c25 and by hig ... | 1992 | 1559984 |
| copurification of small heat shock protein with alpha b crystallin from human skeletal muscle. | immunoreactive alpha b crystallin and a 28-kda protein in an extract of human pectoral muscle were precipitated by (nh4)2so4 at 40% saturation, and coeluted during column chromatography on deae-sepharose and on bio-gel a-5m. the two proteins were separated on a column of s-sepharose hp in the presence of 7 m urea. further chromatography of each of the two resultant fractions on a column of superdex 75 pg and on a tsk-sp 5pw column in the presence of urea yielded preparations of alpha b crystalli ... | 1992 | 1560006 |
| chemical modification of alpha-bungarotoxin with bifunctional reagent, 1,5-difluoro-2,4-dinitrobenzene. | alpha-bungarotoxin (alpha-butx) was modified with a bifunctional reagent, 1,5-difluoro-2,4-dinitrobenzene (dfdnb) to prepare the cross-linked derivatives. the modified products, obtained from the reaction at ph 8.5 with a 2-fold molar excess of dfdnb, were purified successively on a sephadex g-50 and a sp-sephadex c-25 columns, and, fast protein liquid chromatography (fplc) on a mono s column. the preparation was proved to be a homogeneous and dimeric form which arose from the intermolecular cro ... | 1992 | 1560470 |
| [sepsis due to bacillus sp. in a female patient with acute leukemia]. | | 1992 | 1560710 |
| alterations in postnatal intestinal function during chronic hypoxemia. | growth failure is a major complication of chronic hypoxemia, as seen in infants and children with cyanotic congenital heart disease. to determine whether chronic hypoxemia during infancy affects the gastrointestinal tract, we examined small intestinal growth and digestive enzyme activities in chronically hypoxemic newborn lambs and in age-matched controls. chronic hypoxemia was produced by placing an inflatable occluder around the main pulmonary artery and performing a balloon atrial septostomy. ... | 1992 | 1561008 |
| prevalence of coccidia in pigs in zimbabwe. | faecal specimens from 162 piglets, aged 1-5 months, from four farms around harare were examined for coccidia during 1989-1990. oocyst count per gram (opg) of faeces was determined by the mcmaster technique. identification of eimeria species was done after sporulation of oocysts in 2.5% potassium dichromate solution. measurements of sporulated oocysts were taken from 20 positive cases by using calibrated eye micrometer. overall incidence was 25.3% (41/162) varying between 15 and 39.5% at differen ... | 1992 | 1561754 |
| sarcosporidian infection in pigs in uruguay. | examination of enzymatic digests of samples of the crux diaphragm obtained at abbatoirs in montevideo, uruguay, indicated that 57.2% of 269 pigs weighing 90-140 kg were infected with sarcocystis sp. the morphology of sarcocystis in h & e stained sections indicated that they were sarcocystis miescheriana. | 1992 | 1561758 |
| substrate specificity and kinetic properties of pepstatin-insensitive carboxyl proteinase from pseudomonas sp. no. 101. | the substrate specificity of the pepstatin-insensitive carboxyl proteinase isolated from pseudomonas sp. no. 101 was studied by using a series of synthetic chromogenic substrates with general structure p5-p4-p3-p2-p1 *(no2)phe-arg-leu (p5, p4, p3, p2, p1: a variety of amino acids, (no2)phe is p-nitro-l-phenylalanine). the nature of the residues occupying the p2, p3 and p4 positions as well as p1 position had strong influences on kinetic parameters. among those tested, lys-pro-ile-glu-phe*(no2)ph ... | 1992 | 1562589 |
| primary structure differences of human surfactant-associated proteins isolated from normal and proteinosis lung. | the primary structures of human pulmonary surfactant-associated proteins sp-a, sp-b and sp-c isolated from lung lavage of patients with alveolar proteinosis exhibit significant differences from lung surfactant proteins isolated from lungs of healthy individuals. in contrast to sp-a from normal lungs, proteinosis sp-a was shown by sds gel electrophoresis to contain large amounts of unreducibly cross-linked beta chains. specific primary structure modifications of sp-c and sp-b proteins were establ ... | 1992 | 1562613 |
| purification and properties of extracellular mutacin, a bacteriocin from streptococcus sobrinus. | mutacin mt6223, a cell-free bacteriocin produced by streptococcus sobrinus mt6223, was purified by ammonium sulphate precipitation, chromatofocusing with pbe 94 and column chromatography on sp sephadex c-25. the specific activity of the purified mutacin was increased 1950-fold with a recovery of 9.7%. the molecular mass of the purified mutacin preparation was estimated to be 6.5 kda. the mutacin activity was stable from ph 2-7, and was resistant to treatment at 100 degrees c for 20 min. it was i ... | 1992 | 1564438 |
| expression and transport of rabbit surfactant protein a in cos-1 cells. | sv40-transformed green monkey kidney (cos-1) cells were transfected with expression plasmids that contained either the structural gene or cdna for surfactant protein a (sp-a), a major protein of rabbit lung surfactant. the transfected cos-1 cells synthesized several isoforms of sp-a that were found to be less acidic than those produced in rabbit lung tissue. sp-a species with apparent molecular weight (m(r)) approximately equal to 29,000-33,000 were detected in the transfected cells, whereas gly ... | 1992 | 1566859 |
| effects of anoxia on the cochlear summating potential in the guinea pig. | the cochlear summating potential (sp) was recorded extracochlearly from the apex and the round window in 25 guinea pigs, applying anoxia during periods of 1-2 min. for 2 and 8 khz tone pips at 80 and 60 db spl, respectively, the initially negative sp at the apex diminished and even became positive during oxygen cut-off. after restoration of oxygenation, the change in sp reversed direction and was followed by a large negative undershoot. at the round window, the initially positive sp evoked by 8 ... | 1992 | 1567611 |
| truncation of the cooh-terminal domain of the psbe gene product in synechocystis sp. pcc 6803: requirements for photosystem ii assembly and function. | the cooh-terminal domain of the 80-residue cytochrome b559 alpha-subunit (psbe gene product) in synechocystis sp. pcc 6803 was sequentially truncated in order to determine the minimum polypeptide length needed for function and assembly. a stop codon was introduced into the arg-50, arg-59, or tyr-69 codons of the psbe gene, generating mutants truncated by 31, 22, and 12 residues, respectively. removal of 12 residues caused a decrease of 20% in psii function. truncation of 22 or 31 residues caused ... | 1992 | 1567853 |
| surfactant protein c precursor is palmitoylated and associates with subcellular membranes. | surfactant protein c (sp-c) is a 3.7 kda, hydrophobic protein that enhances the adsorption of phospholipids in pulmonary surfactant. sp-c is generated by proteolytic processing of a 21 kda precursor. murine fetal lung explant cultures and a chinese hamster ovary cell line expressing recombinant human sp-c gene (cho/spc) were used to determine the subcellular location and post-translational modification(s) of prosp-c. after in vitro translation, prosp-c of mr = 21,000 was generated. prosp-c was a ... | 1992 | 1567893 |
| human surfactant polypeptide sp-b. disulfide bridges, c-terminal end, and peptide analysis of the airway form. | human hydrophobic surfactant polypeptide, sp-b, purified from lung tissue by exclusion chromatography in organic solvents, has been characterized. the polypeptide is 79 residues long, has a c-terminal methionine, and contains seven cys residues. native human sp-b lacks free thiol groups. three intrachain disulfide bridges were defined, linking cys8 to cys77, cys11 to cys71 and cys35 to cys46. the remaining cys48 is concluded to link the protein chains into homodimers via an interchain disulfide ... | 1992 | 1568474 |
| light-induced oxidation of iron atoms in a photosensitive nitrile hydratase. | the photoactivation process of a photosensitive nitrile hydratase (nhase) from rhodococcus sp. n-771 has been investigated by 57fe mössbauer spectroscopy and magnetic susceptibility measurements in order to clarify the behavior of iron atoms in the enzyme. mössbauer spectra of inactive nhase gave two symmetric-doublet components indicating the presence of two iron species, while that of the active nhase gave a single symmetric doublet indicating the presence of a single iron species. magnetic su ... | 1992 | 1568476 |
| enterocytozoon bieneusi infection in acquired immunodeficiency syndrome-related sclerosing cholangitis. | acalculous cholecystitis and sclerosing cholangitis due to cryptosporidium sp, and cytomegalovirus have been described in patients with the acquired immunodeficiency syndrome (aids). however, in about 40% of cases of aids-related biliary disease, no opportunistic pathogen is identified. the current case report describes the first case, to the best of the authors' knowledge, of aids-related sclerosing cholangitis associated with microsporidiosis. enterocytozoon bieneusi was detected in the duoden ... | 1992 | 1568589 |
| sound induced displacement response of the guinea pig hearing organ and its relation to the cochlear potentials. | the sound induced motion of the cells within the fourth turn of the guinea pig organ of corti was studied in an in vitro preparation (ulfendahl et al. 1989). the cells were visualised by relief microscopy, achieved by an oblique illumination technique. the motion of the sensory cells was observed during the recording of the extracellular receptor potentials; the cochlear microphonics (cm) and the summating potential (sp). our results show that the temporal bone preparation sustains an endocochle ... | 1992 | 1568939 |
| cloning and sequencing of a gene encoding a novel extracellular neutral proteinase from streptomyces sp. strain c5 and expression of the gene in streptomyces lividans 1326. | the gene encoding a novel milk protein-hydrolyzing proteinase was cloned on a 6.56-kb ssti fragment from streptomyces sp. strain c5 genomic dna into streptomyces lividans 1326 by using the plasmid vector pij702. the gene encoding the small neutral proteinase (snpa) was located within a 2.6-kb bamhi-ssti restriction fragment that was partially sequenced. the molecular mass of the deduced amino acid sequence of the mature protein was determined to be 15,740, which corresponds very closely with the ... | 1992 | 1569011 |
| diverse substrate range of a flavobacterium pentachlorophenol hydroxylase and reaction stoichiometries. | an understanding of the enzymatic reactions catalyzing the degradation of substituted phenols, a major group of environmental pollutants, is required for the development of biological methods for the decontamination of halophenol-polluted sites. we found that a flavomonooxygenase, pentachlorophenol hydroxylase, isolated from a flavobacterium sp., catalyzed a primary attack on a broad range of substituted phenols, hydroxylating the para position and removing halogen, nitro, amino, and cyano group ... | 1992 | 1569020 |
| studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: i. isolation and some properties of the glycoproteins. | an acidic heteropolysaccharide preparation derived from the mycelium of fusarium sp. m7-1 was fractionated into two fractions, precipitable and nonprecipitable, by treatment with cetyltrimethylammonium bromide (cetavlon). these two fractions were further purified to apparent homogeneity on ultracentrifugation by treatment with charcoal and gel filtration chromatographies. two glycoproteins, precipitable gp i and nonprecipitable gp ii, were obtained. the molecular weights of gp i and gp ii were e ... | 1992 | 1569046 |
| studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: ii. the primary structures of the low molecular weight carbohydrate chains of the glycoproteins. | the primary structures of the o-glycosidically linked oligosaccharides isolated from glycoproteins gp i and gp ii of fusarium sp. m7-1 were established. the oligosaccharides released by alkaline borohydride treatment from the glycoproteins were purified by bio-gel p-4 and hplc. this approach resulted in one monosaccharide and seven oligosaccharides. their primary structures were resolved mainly by nmr spectrometry in combination with methylation mass spectrometry and fast atom bombardment mass s ... | 1992 | 1569047 |
| studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: iii. the primary structures of the acidic polysaccharides of the glycoproteins. | two o-glycosidically linked acidic polysaccharides, ap i and ap ii, were released, respectively, from glycoproteins gp i and gp ii of fusarium sp. m7-1 by alkaline borohydride treatment and purified by gel filtration chromatography. they were found to be apparently homogeneous on gel filtration chromatography and analytical ultracentrifugation. their molecular weights were estimated to be 8.2 x 10(4) and 3.1 x 10(4), respectively. the various oligosaccharide fragments obtained from ap i and ap i ... | 1992 | 1569048 |
| glutamyl-trna reductase from escherichia coli and synechocystis 6803. gene structure and expression. | in the cyanobacterium synechocystis sp. pcc 6803 and in the enterobacterium escherichia coli delta-amino-levulinic acid (ala) is formed from glutamyl-trna by the sequential action of two enzymes, glutamyl-trna reductase (glutr) and glutamate-1-semialdehyde aminotransferase. e. coli has two glutr proteins with sizes of 45 kda (glutr45) and 85 kda (glutr85) (jahn, d., michelsen, u., and söll, d. (1991) j. biol. chem. 266, 2542-2548). the hema gene, isolated from e. coli and several other eubacteri ... | 1992 | 1569081 |
| identification of a cis-acting dna antisilencer element which modulates vimentin gene expression. | vimentin is a tissue-specific, developmentally regulated member of the intermediate filament protein family normally expressed in cells of mesenchymal origin. transcription factors which recognize specific cis-acting elements of the chicken gene include sp-1 and the 95-kda silencer protein which binds to a 40-bp silencer element at -608 (f. x. farrell, c. m. sax, and z. e. zehner, mol. cell. biol. 10:2349-2358, 1990). in this study, we have identified a region upstream of the silencer element wh ... | 1992 | 1569950 |
| comparison of hektoen and salmonella-shigella agar on 6033 stools of human origin submitted for routine isolation of salmonella sp. and shigella sp. | from 1989 through 1991, 6033 stool specimens from patients at orleans hospital (france), were cultured for enteric pathogens. hektoen agar (he) was compared with salmonella-shigella agar (ss) after direct streaking and after overnight enrichment in mueller-kauffmann broth. yersinia strains were not computed. seven strains of salmonella serotype typhi were isolated, on both media, and excluded from computations for discussed reasons. out of a total of 9 shigella strains, he isolated 9 and ss isol ... | 1992 | 1570178 |
| effect of hypodermin a, an enzyme secreted by hypoderma lineatum (insect oestridae), on the bovine immune system. | the absence of any inflammatory reaction around the first instar larvae (l1) of hypoderma sp. in previously uninfested cattle suggested that these larvae may escape the non-specific defence system of the host. immunosuppression had been noted during an experimental infestation. the aim of this work was to determine more precisely the potential role of hypodermin a (ha), an enzyme secreted by the larvae, in this immunosuppression. ha was found to have no effect on unstimulated lymphocytes from na ... | 1992 | 1570677 |
| effect of swimming on prednisolone-induced osteoporosis in elderly rats. | we investigated the possible ameliorating and preventive effect of swimming on prednisolone-induced osteoporosis in elderly rats. a total of 48 female sabra strain rats were randomly assigned to the following groups and treatments: (1) control (c), (2) swimming (s), (3) prednisolone-treated (cp), and (4) swimming + prednisolone (sp). an additional 8 rats were sacrificed and examined at the onset of the study. groups c and s were sham injected; groups cp and sp were injected with prednisolone (ul ... | 1992 | 1570761 |
| electrocochleography in the presence and absence of perilymphatic fistula. | the purpose of the present study was to evaluate the effect of an acute perilymphatic fistula on the amplitude ratio (sp/ap) of the summating potential (sp) and action potential (ap). the effect of the acute fistula on ap threshold was also addressed. electrocochleographic recordings were obtained before and immediately after surgical laceration of the round window membrane in 19 guinea pigs. stimuli comprised clicks and 2,000-hz and 8,000-hz tone bursts, presented initially at 100 db peak equiv ... | 1992 | 1570934 |
| effects of pulmonary surfactant and surfactant protein a on phagocytosis of fractionated alveolar macrophages: relationship to starvation. | pulmonary surfactant isolated from bronchoalveolar lavage fluid of rat lung contained a high content of surfactant protein a (sp-a) in starved for 2 days compared to fed controls, but this phenomena returned to baseline following more than 4 days starvation. as determined by immunoperoxidase staining of lung sections using sp-a antibody, sp-a could be consistently observed in nonciliated bronchiolar (clara) cells, alveolar type ii cells and some alveolar macrophages (am). fc receptor-mediated ph ... | 1992 | 1571941 |
| prenatal development of gaba-immunoreactive neurons in the human striate cortex. | the prenatal development of neurons immunoreactive to gamma-aminobutyric acid (gaba) in the striate cortex (area 17) of human foetuses aged from 14 weeks to term was studied immunocytochemically. in the 14 week foetus gaba-immunoreactive cells occurred in all layers of area 17 with the highest density in the marginal zone (mz), subplate (sp), deep intermediate zone (iz) and ventricular zone (vz). the cortical plate (cp), which gives rise to most of the definitive adult cortical layers, had relat ... | 1992 | 1572064 |
| viability of fungal cultures maintained at -70 degrees c. | one thousand four hundred forty-seven clinical and environmental isolates of molds, yeasts, aerobic actinomycetes, and algae belonging to 164 genera (382 taxa) maintained on potato dextrose agar at -70 degrees c for periods ranging from 6 months to 13 years were subcultured and then incubated at 25 degrees c to determine their viabilities. thirty-three isolates, alternaria alternata (n = 1), apophysomyces elegans (n = 1), bipolaris spicifera (n = 1), blastomyces dermatitidis (n = 4), cokeromyces ... | 1992 | 1572955 |
| lectin activity of the major surfactant protein (sp-a) may participate in, but is not required for, binding to rat type ii cells. | pulmonary surfactant is a complex mixture of lipids and proteins, of which surfactant protein a (sp-a) is the most abundant glycoprotein. the sp-a molecule has several distinct structural features that include a lectin-like domain, sharing structural features with other mammalian lectins. we have tested the hypothesis that lectin activity of the sp-a molecule is required for the binding to its receptor on the surface of alveolar type ii cells. by using colloidal gold immunocytochemistry in conju ... | 1992 | 1573247 |
| metabolism of dibenzo-p-dioxin by sphingomonas sp. strain rw1. | in the course of our screening for dibenzo-p-dioxin-utilizing bacteria, a sphingomonas sp. strain was isolated from enrichment cultures inoculated with water samples from the river elbe. the isolate grew with both the biaryl ethers dibenzo-p-dioxin and dibenzofuran (df) as the sole sources of carbon and energy, showing doubling times of about 8 and 5 h, respectively. biodegradation of the two aromatic compounds initially proceeded after an oxygenolytic attack at the angular position adjacent to ... | 1992 | 1575472 |
| purification and characterization of an endoglucanase from streptomyces lividans 66 and dna sequence of the gene. | the endoglucanase isolated from culture filtrates of streptomyces lividans iaf74 was shown to have an mr of 46,000 and a pi of 3.3. the specific enzyme activity of 539 iu/mg, determined by the reducing assay method on carboxymethyl cellulose, is among the highest reported in the literature. the cellulase showed typical endo-type activity when reacting on oligocellodextrins. optimal enzyme activity was obtained at 50 degrees c and ph 5.5. the kinetic constants for this endoglucanase, determined w ... | 1992 | 1575483 |
| desulfurization of dibenzothiophene by corynebacterium sp. strain sy1. | strain sy1, identified as a corynebacterium sp., was isolated on the basis of the ability to utilize dibenzothiophene (dbt) as a sole source of sulfur. strain sy1 could utilize a wide range of organic and inorganic sulfur compounds, such as dbt sulfone, dimethyl sulfide, dimethyl sulfoxide, dimethyl sulfone, cs2, fes2, and even elemental sulfur. strain sy1 metabolized dbt to dibenzothiophene-5-oxide, dbt sulfone, and 2-hydroxybiphenyl, which was subsequently nitrated to produce at least two diff ... | 1992 | 1575493 |
| efficient electric field-induced generation of hybridomas from human b lymphocytes without prior activation in vitro. | in this study we investigated whether electrofusion would enable efficient hybridization of human b cells to occur without the need of prior activation in vitro. two cell lines, the murine myeloma sp 2/0 and murine-human heteromyeloma f3b6, were used as fusion partners, and hybridization of human b cells purified either from peripheral blood or from solid lymphoid tissue (tonsil) was studied. optimal hybridization frequencies were obtained at a poration field strength of 3 to 4 kv/cm. electrofus ... | 1992 | 1576322 |
| retroperitoneal necrotizing fasciitis. | necrotizing fasciitis is a mixed infection of the skin and subcutaneous tissues with a characteristic clinical and pathological appearance. early radical surgical excision of all affected tissue is the treatment of choice. in a series of 19 patients with necrotizing fasciitis, bacteriological assessment in 15 confirmed the mixed nature of the infection, with bacteroides sp. isolated from ten patients. all 12 patients who underwent radical surgical excision survived. a subgroup of patients was id ... | 1992 | 1576505 |
| uroporphyrinogen decarboxylase in saccharomyces cerevisiae. hem12 gene sequence and evidence for two conserved glycines essential for enzymatic activity. | the hem12 gene from saccharomyces cerevisiae encodes uroporphyrinogen decarboxylase which catalyzes the sequential decarboxylation of the four acetyl side chains of uroporphyrinogen to yield coproporphyrinogen, an intermediate in protoheme biosynthesis. the gene was isolated by functional complementation of a hem12 mutant. sequencing revealed that the hem12 gene encodes a protein of 362 amino acids with a calculated molecular mass of 41,348 da. the amino acid sequence shares 50% identity with hu ... | 1992 | 1576986 |
| phycobilisome structure in the cyanobacteria mastigocladus laminosus and anabaena sp. pcc 7120. | phycobilisomes of the cyanobacteria mastigocladus laminosus and anabaena sp. pcc7120 differ from typical tricylindrical, hemidiscoidal phycobilisomes in three respects. firstly, size comparisons of the core-membrane linker phycobiliproteins (lcm) in different cyanobacteria by sds/page reveal an apparent molecular mass of 120 kda for the lcm of m. laminosus and anabaena sp. pcc7120. this observation suggests that the polypeptides of these species have four linker-repeat domains. secondly, phycobi ... | 1992 | 1577008 |
| structure of the genes encoding the rod-core linker polypeptides of mastigocladus laminosus phycobilisomes and functional aspects of the phycobiliprotein/linker-polypeptide interactions. | the 3' portion of the cpc operon in mastigocladus laminosus encloses the genes 5'-cpcf-cpcg1-cpcg2-cpcg3 3'. the three cpcg genes encode different phycocyanin-associated rod-core linker polypeptides of the phycobilisomes with predicted 279, 247 and 254 amino acids in length. the gene products cpcg show a high similarity at their n-terminal domains (190 amino acids) and an overall identity of 47-53% to one another. each of the three cpcg polypeptides is highly related to one of the four cpcg gene ... | 1992 | 1577010 |
| galacardins a and b, new glycopeptide antibiotics. | a strain of actinomycetes identified as saccharothrix sp. sank 64289 was found to produce new antibiotics, galacardins a and b. their physico-chemical properties showed that they were new members of glycopeptide antibiotics. they were structurally related to beta-avoparcin but differed from it only in sugar composition. though beta-avoparcin does not contain galactose, galacardins a and b did contain two and one moles of galactose, respectively. they showed strong antimicrobial activity against ... | 1992 | 1577657 |
| a new tetracycline antibiotic with antitumor activity. i. taxonomy and fermentation of the producing strain, isolation and characterization of sf2575. | a new antitumor antibiotic sf2575 has been isolated from a culture filtrate of streptomyces sp. sf2575. the molecular formula was determined to be c40h43no15 by elemental analysis, mass and 13c nmr spectral analyses. the spectral data revealed sf2575 to be a new tetracycline antibiotic. it was active against gram-positive bacteria and exhibited antitumor activity against p388 leukemia in mice. | 1992 | 1577660 |
| cp-82,009, a potent polyether anticoccidial related to septamycin and produced by actinomadura sp. | a new polyether antibiotic cp-82,009 (c49h84o17) was isolated by solvent extraction from the fermentation broth of actinomadura sp. (atcc 53676). following purification by column chromatography and crystallization, the structure of cp-82,009 was elucidated by spectroscopic (nmr and ms) methods. the absolute stereochemistry was determined by a single crystal x-ray analysis of the corresponding rubidium salt. cp-82,009 is among the most potent anticoccidial agents known, effectively controlling th ... | 1992 | 1577662 |
| specificity of lung surfactant protein sp-a for both the carbohydrate and the lipid moieties of certain neutral glycolipids. | binding specificity of the major surfactant protein sp-a from human and dog lung has been investigated. radiobinding experiments have shown that both proteins bind in a ca(2+)-dependent manner to galactose, mannose, fucose, and glucose linked to bovine serum albumin. these results are in accord with a previous study in which monosaccharides were linked to agarose (haagsman, h. p., hawgood, s., sargeant, t., buckley, d., white, r. t., drickamer, k., and benson, b. j. (1987) j. biol. chem. 262, 13 ... | 1992 | 1577827 |
| self-splicing introns in trna genes of widely divergent bacteria. | the organization of eukaryotic genes into exons separated by introns has been considered as a primordial arrangement but because it does not exist in eubacterial genomes it may be that introns are relatively recent acquisitions. a self-splicing group i intron has been found in cyanobacteria at the same position of the same gene (that encoding leucyl transfer rna, uaa anticodon) as a similar group i intron of chloroplasts, which indicates that this intron predates the invasion of eukaryotic cells ... | 1992 | 1579169 |
| purification and characterization of aspmd1, an isoschizomer of sau3ai, from a marine bacterium, alcaligenes sp md1. | | 1992 | 1579507 |
| an osteosarcoma cell line with hypodiploid chromosome number and complex structural anomalies. | giemsa-banding analysis performed on a newly established metastatic osteosarcoma cell line (sp-388) that was passaged through the nude mouse revealed a hypodiploid stemline number of 39 chromosomes. this cell line showed both numerical and structural abnormalities, including the abnormally banded region in certain marker chromosomes. chromosomes 3, 15, 17 and the x were the most frequently absent elements of this tumor. segments of chromosomes 3, 15 and x could not be identified even in rearrang ... | 1992 | 1580551 |
| in vitro cytotoxicity of the conjugate adriamycin with anti-sarcoma monoclonal antibody 19-24. | monoclonal antibody (moab) 19-24, which recognizes a cell surface sarcoma-associated antigen p102, was linked via a biotin-avidin-biotin bridge to adriamycin (adr). the molar ratios of adr: total protein ranged from 2 to 7.5. significant differences were not observed between the binding ability of the adr-moab conjugate and of the unconjugated moab 19-24 to fresh sarcoma tissue membranes. the adr-moab conjugate also retained the ability to compete with moab 19-24 for binding to sarcoma-associate ... | 1992 | 1580569 |
| pantoea punctata sp. nov., pantoea citrea sp. nov., and pantoea terrea sp. nov. isolated from fruit and soil samples. | a total of 37 bacterial strains with the general characteristics of the family enterobacteriaceae were isolated from fruit and soil samples in japan as producers of 2,5-diketo-d-gluconic acid from d-glucose. these organisms were phenotypically most closely related to the genus pantoea (f. gavini, j. mergaert, a. beji, c. mielearek, d. izard, k. kersters, and j. de ley, int. j. syst. bacteriol. 39:337-345, 1989) and were divided into three phenotypic groups. we selected nine representative strain ... | 1992 | 1581180 |
| mycoplasma phocidae sp. nov., isolated from harbor seals (phoca vitulina l.). | in 1979 and 1980, more than 400 harbor seals (phoca vitulina) along the new england coast of the united states died of epizootic pneumonia that was attributed to an influenza virus. six mycoplasma isolates that were recovered from the respiratory tracts of affected seals were investigated and were found to be serologically identical and distinct from previously described species. these isolates required serum for growth, did not possess a cell wall, and did not hydrolyze urea. arginine was hydro ... | 1992 | 1581181 |
| reclassification of two strains of arthrobacter oxydans and proposal of arthrobacter nicotinovorans sp. nov. | arthrobacter oxydans dsm 419 and dsm 420 have chemical and microbiological properties that are consistent with assignment to the genus arthrobacter. both organisms have the lysine-alanine-threonine-alanine peptidoglycan type. dna-dna pairing studies indicated that a. oxydans dsm 419 should be reclassified as arthrobacter ureafaciens and that a. oxydans dsm 420t forms the nucleus of a distinct genomic species. we propose that a. oxydans dsm 420 should be reclassified as arthrobacter nicotinovoran ... | 1992 | 1581183 |
| proposal of mycobacterium peregrinum sp. nov., nom. rev., and elevation of mycobacterium chelonae subsp. abscessus (kubica et al.) to species status: mycobacterium abscessus comb. nov. | we studied the taxonomic positions of the rapidly growing organism mycobacterium fortuitum and phenotypically related organisms. we confirmed that "mycobacterium peregrinum" atcc 14467t (t = type strain) is genetically independent of m. fortuitum atcc 6841t by using various dna hybridization conditions. strains that were genetically identified as "m. peregrinum" were phenotypically differentiated from m. fortuitum atcc 6841t. thus, we propose that "m. peregrinum" should be revived as an independ ... | 1992 | 1581184 |
| ehrlichia ewingii sp. nov., the etiologic agent of canine granulocytic ehrlichiosis. | the 16s rrna gene was amplified, cloned, and sequenced from the blood of two dogs that were experimentally infected with the etiologic agent of canine granulocytic ehrlichiosis. the 16s rrna sequence was found to be unique when it was compared with the sequences of other members of the genus ehrlichia. the most closely related species were ehrlichia canis (98.0% related) and the human ehrlichiosis agent (ehrlichia chaffeensis) (98.1% related); all other species in the genus were found to be phyl ... | 1992 | 1581189 |
| rickettsia japonica sp. nov., the etiological agent of spotted fever group rickettsiosis in japan. | we propose the name rickettsia japonica sp. nov. (with type strain yh [= atcc vr-1363]) for a serologically specific species of spotted fever group rickettsiae that are pathogenic for humans (j. infect. dis. 159:1122-1126, 1989; j. clin. microbiol. 28:1177-1180, 1990). the biologic and genomic characteristics of the organism (g+c content, 31.2 +/- 0.7 mol%) are essentially the same as those of other pathogenic spotted fever group rickettsiae, although the r. japonica isolates cause a persistent ... | 1992 | 1581190 |
| proposal of chlamydia pecorum sp. nov. for chlamydia strains derived from ruminants. | chlamydia pecorum sp. nov. is proposed as the fourth species of the genus chlamydia on the basis of the results of a genetic analysis of chlamydia strains that were isolated from cattle and sheep which had various diseases, including sporadic encephalitis, infectious polyarthritis, pneumonia, and diarrhea. the levels of dna-dna homology between c. pecorum and strains of c. psittaci, chlamydia pneumoniae, and chlamydia trachomatis were less than 10%. several dna probes were used to identify c. pe ... | 1992 | 1581191 |
| coding sequence of a heterocyst ferredoxin gene (fdxh) isolated from the nitrogen-fixing cyanobacterium calothrix sp. pcc 7601. | | 1992 | 1581559 |
| nucleotide sequence of the phytoene desaturase gene from synechocystis sp. pcc 6803 and characterization of a new mutation which confers resistance to the herbicide norflurazon. | | 1992 | 1581575 |
| traditional chinese medical treatment of heart disease. | western conventional approaches have limited value in the treatment of cardiac diseases because they do not address the true causes of the problems and focus only on treating the heart and lungs. little attention is paid to the organ systems involved (ki, sp, liv) and the significance of symptoms seemingly unrelated to cardiopulmonary pathology. acupuncture used in conjunction with conventional medications (diuretics, beta-blockers, peripheral dilators, etc.) can greatly enhance results. the use ... | 1992 | 1581650 |
| acupuncture for immune-mediated disorders. literature review and clinical applications. | acupuncture activates the defense systems. it influences specific and nonspecific cellular and humoral immunities; activates cell proliferation, including blood, reticuloendothelial, and traumatized cells; and activates leucocytosis, microbicidal activity, antibodies, globulin, complement, and interferon. it modulates hypothalamic-pituitary control of the autonomic and neuroendocrine systems, especially microcirculation, response of smooth and striated muscle, and local and general thermoregulat ... | 1992 | 1581655 |
| trace metals in limpets (patella sp) from the coast of santa cruz de tenerife (canary islands). | | 1992 | 1581679 |
| bacteria that degrade p-chlorophenol isolated from a continuous culture system. | two gram-positive coryneform bacteria that degraded p-chlorophenol isolated from a continuous culture system are characterized. isolate b (probably and arthrobacter sp.) completely removed the p-chlorophenol from a medium with a concomitant increase in cell density within 16 h. isolate f similarly removed the p-chlorophenol within 28 h but without an increase in cell density. isolates b and f also removed the p-chlorophenol from a medium with p-chlorophenol as the sole carbon source within 32 an ... | 1992 | 1581863 |