| rapid identification of cultured mycobacterium tuberculosis with a panel of monoclonal antibodies in western blot and immunofluorescence. | this paper describes the identification of cultured mycobacteria with a panel of monoclonal antibodies directed against species-specific epitopes in a western blot (wb) test and in an immunofluorescence test (ift). in wb, we identified mycobacteria of the mycobacterium tuberculosis complex (m. tuberculosis, m. africanum, m. bovis, m. bovis bcg, and m. microti) with 10(8) bacteria. in the ift, we identified mycobacteria of the m. tuberculosis complex, the m. avium complex (m. avium, m. intracellu ... | 1989 | 2483276 |
| a 180-kilodalton protein from mycobacterium tuberculosis defined by a human t cell clone. | a high molecular weight protein from mycobacterium tuberculosis (m. tuberculosis) has been identified, that is recognized by peripheral blood mononuclear cells from several tuberculous patients and by a t cell clone derived from a patient with tuberculous pleurisy. purification of this fraction demonstrated biological activity to reside in a 180-kda protein component. this mycobacterial protein appears to exist in some, but not all mycobacteria as the clone reacts to m. tuberculosis, bcg m. kans ... | 1989 | 2483413 |
| the origin and specificity of intrathecal igg in chronic relapsing experimental allergic encephalomyelitis. | the source of igg in the cerebrospinal fluid (csf) in guinea pigs with chronic relapsing experimental allergic encephalomyelitis (cr-eae) was investigated using quotient analysis of total igg and albumin concentrations and by computing csf-plasma ratios of specific igg concentrations. increased blood-csf barrier (b-csfb) permeability was shown by elevated albumin quotients in both relapse and remission phases of cr-eae and intrathecal production of igg was indicated by raised ratios of igg to al ... | 1989 | 2485078 |
| [congenital tuberculosis]. | congenital tuberculosis is a rare disease of variable incidence in accordance with prevalence of the disease in the general population. we report a case in a neonate born prematurely, whose mother presented with miliary tuberculosis diagnosed in the puerperal period. the newborn had no contact with his mother after delivery, neither with other infected people. the clinical picture consisted mainly in signs and symptoms of septicaemia of gradual onset. mycobacterium tuberculosis was isolated from ... | 1989 | 2485525 |
| homology of the 70-kilodalton antigens from mycobacterium leprae and mycobacterium bovis with the mycobacterium tuberculosis 71-kilodalton antigen and with the conserved heat shock protein 70 of eucaryotes. | two lambda gt11 recombinant clones, jkl2 and jkl15, each containing an insert coding for part of the highly immunogenic 70-kilodalton (kda) protein antigen, were isolated from a mycobacterium leprae genomic library by immunoscreening with the monoclonal antibody l7. clone jkl2 contained the largest insert, 2.3 kilobase pairs. nonoverlapping fragments of this insert were used as probes and showed strong hybridization to a number of mycobacterium tuberculosis-lambda gt11 recombinants producing pro ... | 1989 | 2491836 |
| psoriasiform plaque on the buttock. tuberculosis verrucosa cutis. | | 1989 | 2491938 |
| an outbreak of tuberculosis in rural delaware. | a total of 35 patients diagnosed with tuberculosis in 1976-1984 were identified as part of a sustained outbreak involving five communities located in southern delaware. the index case, a 25-year-old black female with sputum smear-positive, cavitary pulmonary tuberculosis, was a sexual partner of three other persons with cavitary pulmonary tuberculosis and a close contact of 10 other persons with tuberculosis. the median age of the 35 patients was 23 years (range 15 months to 77 years), and 13 (3 ... | 1989 | 2492143 |
| t-cell-mediated protection of mice against virulent mycobacterium tuberculosis. | we sought to protect cba mice against tuberculosis using in vivo transfer of a t-cell line previously shown to be capable of i-a-restricted recognition of peritoneal macrophages infected in vitro with mycobacterium tuberculosis. this line induces total bacteriostasis in vitro. in mice that received 500 rads of irradiation 48 h before infection, the t-cell line caused significant prolongation of life when given intravenously with a challenge dose of 5 x 10(6) organisms. similar experiments with t ... | 1989 | 2492259 |
| gen-probe test should not be considered final in mycobacterium tuberculosis identification. | | 1989 | 2492302 |
| 1,25(oh)2-vitamin d3 synergizes with pyrazinamide to kill tubercle bacilli in cultured human macrophages. | pyrazinamide (pza) is believed to be mycobactericidal in vivo. because it is ineffective at neutral ph in vitro, it is thought to owe its in vivo activity at least partly to acting upon tubercle bacilli (tb) in the helpfully low ph of macrophage (mp) phagolysosomes. however, when it was tested in tb-infected cultured human mp, it was not bactericidal and was able only to slow intra-mp bacillary growth. recent evidence has suggested that human mp need hormonal support from certain vitamin d metab ... | 1989 | 2492412 |
| [tuberculosis, a continuing challenge]. | | 1989 | 2492642 |
| inhibition by retinoic acid of multiplication of virulent tubercle bacilli in cultured human macrophages. | the immunologically active vitamin retinoic acid (ra) was tested for the ability to increase the resistance of cultured human macrophages (mp) to experimental infection with virulent mycobacterium tuberculosis erdman (tubercle bacilli [tb]). it was added to mp in various concentrations and addition regimens. protection against tb was measured by counting live tb (cfu) in lysates of samples of mp taken at 0, 4, and 7 days after mp infection. ra was protective when added after infection at the pha ... | 1989 | 2492972 |
| a 25-kilodalton fraction from mycobacterium tuberculosis that inhibits hexose monophosphate shunt activity, lysozyme release, and h2o2 production: reversal by gamma interferon. | this study examined the effects of a 25-kilodalton (kda) glycolipoprotein derived from mycobacterium tuberculosis on phagocyte functions associated with antimicrobial activity. the 25-kda fraction inhibited the ability of both polymorphonuclear cells and cultured monocytes to release lysozyme and produce hydrogen peroxide. in addition, the glycolipoprotein was capable of reducing hexose monophosphate shunt activity and interfered with the ability of polymorphonuclear cells to reduce nitro blue t ... | 1989 | 2492974 |
| [antitubercular agents--recent developments in assessment and use]. | | 1989 | 2493430 |
| [simultaneous detection of m. tuberculosis and m. avium-m. intracellulare. a contribution to the clinical aspects and diagnosis of mycobacterioses in aids]. | | 1989 | 2493431 |
| suppression of experimental autoimmune diseases and prolongation of allograft survival by treatment of animals with low doses of heparins. | the ability of activated t lymphocytes to penetrate the extracellular matrix and migrate to target tissues was found to be related to expression of a heparanase enzyme (naparstek, y., i. r. cohen, z. fuks, and i. vlodavsky. 1984. nature (lond.). 310:241-243; savion, n., z. fuks, and i. vlodavsky. 1984. j. cell. physiol. 118:169-176; fridman, r., o. lider, y. naparstek, z. fuks, i. vlodavsky, and i. r. cohen. 1987. j. cell. physiol. 130:85-92; lider, o., j. mekori, i. vlodavsky, e. baharav, y. na ... | 1989 | 2493485 |
| case records of the massachusetts general hospital. weekly clinicopathological exercises. case 12-1989. a 24-year-old haitian man with right flank pain and recent fever. | | 1989 | 2493581 |
| nucleotide sequence of the 19 kda antigen gene from mycobacterium tuberculosis. | | 1989 | 2493628 |
| bone marrow in hiv infection. a comparison of fluorescent staining and cultures in the detection of mycobacteria. | fifty-one bone marrow aspirates and biopsies from 47 human immunodeficiency virus-(hiv) infected patients (42 with acquired immune deficiency syndrome [aids], 5 with aids-related complex [arc]) were processed by standard methods for smears and paraffin sections. aspirates were cultured for mycobacteria. the sections, imprints, and smears were examined by fluorescent microscopy with the use of truant's modification of the auramine-rhodamine stain. mycobacterial cultures had positive results from ... | 1989 | 2493737 |
| evaluation of an enzyme-linked immunosorbent assay (elisa) using an igg antibody to mycobacterium tuberculosis antigen 5 in the diagnosis of active tuberculosis in children. | an enzyme-linked immunosorbent assay (elisa) using mycobacterium tuberculosis antigen 5 has been evaluated as a serodiagnostic test for active tuberculosis in children. forty children of either sex, ages 1 to 14 yr, were studied, including 21 bacteriologically confirmed tuberculous patients and 19 control subjects who were evaluated for tuberculosis and found to be free of disease. at a 1:20 dilution, the test showed a sensitivity of 0.857 and a specificity of 1.000, with an accuracy value of 0. ... | 1989 | 2493761 |
| influence of bcg-induced immunity on the bactericidal activity of isoniazid and rifampicin in experimental tuberculosis of the mouse and guinea-pig. | the influence of previous bcg vaccination on the bactericidal activity of isoniazid and rifampicin has been studied using serial counts of viable tubercle bacilli in the spleens and lungs of mice and guinea-pigs infected intravenously with m. tuberculosis, strain h37rv. in mice, bcg vaccination decreased the bactericidal activity of isoniazid in the spleen, but did not affect its activity in the lungs, where immunity is less strongly expressed. bcg did not influence the bactericidal activity of ... | 1989 | 2493801 |
| inhibition by normal human serum of mycobacterium avium multiplication in cultured human macrophages. | normal human serum used as a protein supplement in rpmi 1640 medium inhibited growth in blood-derived human macrophages (mp) of virulent mycobacterium avium serovars 4 and 8, derived from patients with acquired immunodeficiency syndrome, but not virulent mycobacterium tuberculosis. a defined serum substitute (ss) promoted the intramacrophage growth of m. avium but not of m. tuberculosis. the effects of serum or ss were measured by counting viable bacteria in lysates of the mp at 0, 4, and 7 days ... | 1989 | 2494117 |
| [therapeutic efficacy of the principal antitubercular drugs: an evaluation by antibiogram]. | pulmonary and extrapulmonary tuberculosis is still encountered in clinical practice, sometimes in forms that are difficult to categorize nosographically. in this study, the therapeutic efficacy of the 6 main antitubercular drugs (rifampicin, streptomycin, isoniazide, ethambutol, kanamycin, para-aminosalicylic acid) in 20 patients with early tb of the lungs was assessed on the basis of antibiogram findings. para-aminosalicylic acid was found to be the least effective; isoniazide, rifampicin and s ... | 1989 | 2494591 |
| further specific triglycosyl phenol phthiocerol diester from mycobacterium tuberculosis. | mono- and two-dimensional 1h-nmr allowed the structural elucidation of a glycolipid belonging to the phenolic mycosides series: 2,3,4-tri-0-methyl fucopyranosyl (alpha 1----3) rhamnopyranosyl (alpha 1----3) rhamnopyranosyl (alpha 1----dimycocerosyl) phenol phthiocerol. it shares with the major phenolic glycolipid the two terminal sugar residues, suggesting its potential antigenicity. the glycolipid may also represent an intermediate in the biosynthesis of the major one. | 1989 | 2495024 |
| ldh isoenzyme as a tool for the identification of mycobacteria. | using polyacrylamide gel electrophoretic technique, the lactate dehydrogenase (ldh) isoenzyme patterns have been studied in four slow growing mycobacteria viz. mycobacterium tuberculosis, m. avium, m. microti, and m. bovis and four rapid growing mycobacteria viz. m. fortuitum, m. parafortuitum, m. thermoresistible and m. diernhoferi. each mycobacterial species exhibited distinct isoenzyme pattern for ldh. | 1989 | 2495332 |
| mycobacterium tuberculosis transmission in a health clinic--florida, 1988. | | 1989 | 2495425 |
| [immediate and late results of the surgical treatment of patients with post-tuberculosis cavities]. | examination of lung sections from patients with residual posttuberculous cavities not discharging tubercle bacilli for long periods revealed latent bacterial lesions of cavern wall tissues and active specific processes in 73.6 and 92.1 per cent of the cases respectively. bacteriological investigation of the resected lungs revealed no significant advantages of the tuberculostatic therapy with using rifadin at various stages for 2-4-month courses in comparison to the therapy without rifadin. the i ... | 1989 | 2495527 |
| [increasing the effectiveness of microbiological diagnosis of limited nondestructive pulmonary tuberculosis]. | | 1989 | 2495528 |
| features in hospitalized patients with symptom-detected or radiologically-detected pulmonary tuberculosis. | to compare features of tuberculosis detected either clinically or radiologically, we studied 498 patients hospitalized for tuberculosis. tuberculosis was less frequently radiologically-detected in patients over 65 yrs old (p less than 0.01) and in directorial staff and inactive subjects (p less than 0.0001). pleural effusion and radiological cavities were more frequent in the symptom-detected group, but half of the radiologically-detected patients had clinical symptoms which could have led to th ... | 1989 | 2495981 |
| mycobacterial esophagitis in aids. | a patient with acquired immune deficiency syndrome (aids) who presented with dysphagia is described. barium swallow demonstrated diffuse esophagitis with longitudinal ulceration and sinus tracts to the mediastinum. mycobacteria were seen on esophageal biopsies and mycobacterium tuberculosis was cultured from a pleural effusion. mycobacterial esophagitis should be considered in the differential diagnosis of esophagitis in aids, particularly when sinus tracts are demonstrated. | 1989 | 2496001 |
| defects in sera from acquired immunodeficiency syndrome (aids) patients and from non-aids patients with mycobacterium avium infection which decrease macrophage resistance to m. avium. | some characteristics of the sera and macrophages (mp) of human immunodeficiency virus (hiv)-infected patients which might contribute to their unusual susceptibility to mycobacterium avium infection were studied. cultures of patient peripheral blood mp in medium supplemented with their sera or normal subject sera were infected with m. avium and compared with similar cultures of normal mp. intracellular mycobacterial replication was measured in the infected mp by cfu counts of the bacteria made fr ... | 1989 | 2496029 |
| immune mechanisms in the pathogenesis of pulmonary tuberculosis. | the pathogenesis of pulmonary tuberculosis and the beneficial and detrimental effects of the host's cell-mediated immune response in this disease are reviewed. tubercle bacilli are hardly facultative intracellular pathogens that live and multiply in nonactivated macrophages. before tubercle bacilli can be destroyed by macrophages, these cells must be activated by t lymphocytes and their lymphokines. such activation is the essence of cell-mediated immunity. cell-mediated immunity also has a detri ... | 1989 | 2496453 |
| regulation of t cell responsiveness against mycobacterial antigens by hla class 2 immune response genes. | helper t lymphocytes can only recognize mycobacterial antigens when they are presented by hla class 2 molecules. thus, these molecules may play an important role in the regulation of the immune response against mycobacteria. in this paper it is demonstrated that the t cells from individuals with different hla class 2 molecules react to different mycobacterial antigens. these data indicate that hla class 2 molecules are the products of immune response (ir) genes for mycobacteria. such genetically ... | 1989 | 2496455 |
| a cloned dna fragment for identification of mycobacterium tuberculosis. | the identification of mycobacterium tuberculosis is a lengthy process. attempts were made to develop a more rapid, specific, and sensitive assay for identifying tubercle bacilli in biologic specimens by differentially screening plasmid libraries of sau3ai-digested m. tuberculosis h37rv (atcc 25618) dna with homologous and heterologous dna for clones that hybridized strongly with m. tuberculosis dna alone. three clones, pmtb1, pmtb2, and pmtb3, were selected for further study. southern analysis i ... | 1989 | 2496456 |
| serology of mycobacteria: characterization of antigens recognized by monoclonal antibodies. | analysis of the antibody response to mycobacterial extracts has identified a limited set of proteins that are recognized as immunodominant in the balb/c strain of mice. detailed characterization has revealed that several of these antigens are homologues of proteins known to be induced in response to environmental stress stimuli in other prokaryotic and eukaryotic cell types. it is proposed that differential gene expression may play a role in determining which antigens are recognized during infec ... | 1989 | 2496457 |
| approaches to the development of new antituberculosis drugs. | in the last two decades, no new major antituberculosis drug has been developed. although dramatic improvements in chemotherapy for tuberculosis have been achieved through careful studies of drug regimens, there is still a need for new agents that are highly active. the antimycobacterial drugs used at present in therapy for tuberculosis were obtained by either blind screening or chemical modification of active compounds, with tentative optimization of chemotherapeutic properties. other approaches ... | 1989 | 2496458 |
| new experimental drugs for the treatment of tuberculosis. | new antitubercular agents are needed for two main purposes: to further simplify therapy (through reductions in the number of medicaments used, the number of doses administered, and the duration of treatment required), thus facilitating supervision and improving compliance, and to combat resistant mycobacteria. reduction in the number of medicaments has been achieved by combining two or more drugs in a single tablet while retaining a degree of bioavailability similar to that of the single compone ... | 1989 | 2496459 |
| [alcoholism and pulmonary tuberculosis]. | a study of concomitant cases of alcoholism and pulmonary tuberculosis is reported, with reference to the incidence of alcoholism among the patients with pulmonary tuberculosis, causative relationships between the two diseases, clinical manifestations and course of pulmonary tuberculosis and alcoholism where the two are combined, as well as the treatment of alcoholism and pulmonary tuberculosis and its organizational aspects. | 1989 | 2496535 |
| the bactec-system in the diagnosis of tuberculosis. comparison of a conventional and the radiometric method (bactec) for culturing, differentiation and susceptibility testing of mycobacteria. | a method developed in the usa (2, 6, 8) for the culturing, identification and differentiation of mycobacteria by means of a radiometric method (bactec) was compared with a conventional method by examining 802 specimens received. obviously better results were obtained with the new method: the number of positive cultures was 102 (12.7%) for bactec against 87 (10.8%) for the conventional method. when using the radiometric method, the contamination rate (5.1%) was higher than for the conventional me ... | 1989 | 2496537 |
| hiv infection in patients with tuberculosis in kinshasa, zaire. | to better define the interrelationship of infection with human immunodeficiency virus (hiv) and tuberculosis (tb), we conducted three hiv serosurveys of inpatients and outpatients with confirmed or suspected tb in kinshasa, zaire. hiv seroprevalence in hospitalized sanatorium patients did not change significantly in serosurveys conducted in 1985 and 1987 (92/231 [40%] versus 85/234 [36%]). these proportions were significantly higher than the 17% hiv seroprevalence observed in a 1987 serosurvey o ... | 1989 | 2496632 |
| presumptive diagnosis and treatment of pulmonary tuberculosis based on radiographic findings. | we analyzed the outcome of therapy for 139 patients who were treated for a presumptive diagnosis of pulmonary tuberculosis based on radiographic abnormalities. patients who had acid-fast bacilli seen on sputum smears and patients who had received adequate therapy for tuberculosis in the past were excluded from the analysis. accuracy of the diagnosis was determined by comparison of clinical and radiographic findings after 3 months of isoniazid, rifampin, and ethambutol, as well as the results of ... | 1989 | 2496633 |
| the epidemiologic patterns of drug-resistant mycobacterium tuberculosis infections: a community-based study. | a community-based study of tuberculosis in santa clara county, california was conducted in order to identify community-specific determinants of drug-resistant mycobacterium tuberculosis infections. from january 1984 through december 1986, 517 verified cases of tuberculosis were reported from the county. drug susceptibility test results to isoniazid, streptomycin, ethambutol, and rifampin were available for 256 of the 517 cases. the frequency of resistance of m. tuberculosis isolates to one or mo ... | 1989 | 2496635 |
| molecular study of the t cell repertoire in family contacts and patients with leprosy. | the specificity of lymphocyte proliferative responses of 22 family contacts and 7 patients with leprosy were analyzed using ag fractions from soluble extracts of mycobacterium leprae and mycobacterium tuberculosis. fractions 10-100 kda m.w. from each extract were separated by sds-polyacrylamide gel electrophoresis, electroblotted to nitrocellulose membrane and solubilized for use in lymphocyte culture. the main immunogenic fractions for both contacts and patients had m.w. of 12,000 to 22,000, 35 ... | 1989 | 2497176 |
| diagnosis of pulmonary infections in patients infected with the human immunodeficiency virus. | over a three-year period, 54 episodes of pneumonia were diagnosed in 45 adults infected with the human immunodeficiency virus (hiv). these episodes were reviewed in order to assess the distribution of pathogens and their clinical presentation. thirty-six episodes were due to an opportunistic pathogen (pneumocystis carinii in 31, mycobacterium avium complex in 3, mycobacterium tuberculosis in 2), and 18 were caused by non-opportunistic pathogens (11 streptococcus pneumoniae, 2 haemophilus influen ... | 1989 | 2498092 |
| impact of protein malnutrition on exogenous reinfection with mycobacterium tuberculosis. | malnutrition may be a predisposing host factor in the development of exogenous-reinfection tuberculosis. outbred hartley guinea pigs were given isocaloric diets containing either 30% ovalbumin (control animals) or 10% ovalbumin (low-protein-fed [lp] animals). equal numbers of control and lp animals were assigned to one of three infection groups: (i) primary pulmonary infection with a low-virulence, streptomycin-resistant (lvsr) isolate of mycobacterium tuberculosis and then reinfection 6 weeks l ... | 1989 | 2498206 |
| changing pattern of cutaneous tuberculosis. a prospective study. | during the past 3 years, we have seen a changing pattern of cutaneous tuberculosis, in which its incidence was recorded to show an upward trend affecting predominantly the adult men. the latter was true in reinfection--lupus vulgaris and tuberculosis verrucosa cutis, whereas a substantial number of children were victims of reactivation--scrofuloderma. the duration of the disease was usually short in reactivation tuberculosis. its clinical expression, however, conformed largely to the ritual text ... | 1989 | 2498216 |
| an hiv-infected man with a neck ulcer and a fever. | | 1989 | 2498360 |
| diagnosis of mycobacterium bacteremia in patients with acquired immunodeficiency syndrome by direct examination of blood films. | thirty acquired immunodeficiency syndrome patients with mycobacterial bacteremia documented by du pont isolator (du pont co., wilmington, del.) blood cultures underwent microscopic examination of buffy coat blood smears. of 30 patients, 14 were culture positive for mycobacterium avium-mycobacterium intracellulare complex and 1 was positive for m. tuberculosis. of 15 culture-positive patients, 13 had identifiable organisms on kinyoun- or auramine-stained direct blood smears. | 1989 | 2498390 |
| screening of serum antibodies against mycobacterium tuberculosis by enzyme-linked immunosorbent assay (elisa) in healthy population. | serum samples of venous blood of healthy individuals, chosen at random from two areas of the people's democratic republic of yemen, were examined for the presence of igg antibodies against mycobacterial antigen by elisa. from the district of aden, the capital (a), there were 214 samples (72% vaccinated by bcg, 108 men and 106 women), other 235 ones originated from mountain area laudar muhairas (h) (66% vaccinated, 115 men and 120 women). the overall average of acquired titres was 1:81.4 in area ... | 1989 | 2498426 |
| promising new drugs in the treatment of tuberculosis and mycobacteriosis. | the aims of this work were to investigate the possible effect of several antimicrobial agents alone and in combinations against 190 clinical isolates of mycobacterium tuberculosis and 30 isolates of mycobacterium avium, 30 mycobacterium fortuitum and 30 mycobacterium chelonei. the susceptibility was determined in müller-hinton agar and middlebrook 7h 10 agar. for interpretation of the results the minimum inhibitory concentration (mic) was determined and for the combinations of antimicrobials we ... | 1989 | 2498468 |
| multiple drug therapy for pediatric tuberculosis. | | 1989 | 2498594 |
| [isolation of mycobacteria l forms as a prognostic criterion of recurrence and aggravation of tuberculosis in patients with extended residual tuberculous lesions of the lungs]. | to elucidate the role of tubercle bacilli l-forms in tuberculosis relapsing and aggravation in patients with extended residual lesions in the lungs, 117 patients mainly with extended and neglected destructive tuberculosis of the lungs were observed for a period of 5 to 7 years with using microbiological and clinico-roentgeno-laboratory examinations. analysis of the immediate and remote results of the treatment showed that in the group of the abacillary patients according to the routine microbiol ... | 1989 | 2498865 |
| [methods of improving bacteriological confirmation in tuberculosis of the respiratory organs]. | | 1989 | 2498866 |
| [microbiological characteristics of residual pulmonary caverns]. | | 1989 | 2498867 |
| [bronchoalveolar lavage in the diagnosis of respiratory tract diseases in children and adolescents]. | | 1989 | 2499061 |
| the diagnostic yield of prebronchoscopy sputa and bronchial washings in patients with biopsy-proven pulmonary tuberculosis. | we report on a series of 35 patients with pulmonary tuberculosis diagnosed by flexible fibre-optic bronchoscopy and transbronchial lung biopsy after 3 sputum specimens had been microscopy-negative. this study re-evaluates this invasive procedure. additional prebronchoscopy specimens of expectorated sputum yielded the diagnosis in 7 of 16 cases (43.8%) on microscopy. sputum culture results were positive in 12 of 33 (36.4%). pleural fluid culture was diagnostic in 1 case, and in another miliary tu ... | 1989 | 2499066 |
| pseudotumoral tuberculosis of the uterine cervix. cytologic presentation. | the cytologic presentation of a case of pseudotumoral tuberculosis of the uterine cervix is described. the presence in cervicovaginal smears of epithelioid cells arranged in clusters mixed with langhans' giant cells was highly suggestive of tuberculosis. this diagnosis was confirmed by the detection of acid-fast bacilli in histologic preparations and cultures from biopsy specimens. | 1989 | 2499147 |
| the 45 kilodalton molecule of mycobacterium tuberculosis identified by immunoblotting and monoclonal antibodies as antigenic in patients with tuberculosis. | the object of this study was to discover new m. tuberculosis antigens which are recognized by patients with tuberculosis, because effective serodiagnostic tests are likely to require combinations of different antigens. in our early experiments using immunoblotting, the findings suggested that human sera from smear-negative tuberculosis patients bound to an antigen in the 45 kda region. subsequently, estimates of molecular weight in the immunoblots confirmed that the murine monoclonal antibody (m ... | 1989 | 2499350 |
| [the immunology of tuberculosis]. | an introductory overview on the present state and future prospect of the immunology of tuberculosis is presented with the following six chapters. 1. contribution of tuberculosis immunologists to the modern immunology. when one reminds koch's phenomenon, freund's adjuvant, and the findings or new ideas of, for instance, cell-mediated transfer of tuberculin allergy, test of mif which was first described as "lymphokines", effector macrophages activated with immune lymphocytes against mycobacterial ... | 1989 | 2499728 |
| [evaluation of various schedules of chemotherapy of newly detected cases of infiltrative pulmonary tuberculosis]. | | 1989 | 2499881 |
| [studies on the virulence in the guinea pig of tuberculosis bacilli isolated from patients with pulmonary tuberculosis before treatment in shanghai]. | the virulence in guinea-pig of 44 tubercle bacilli cultures from patients of tuberculosis before treatment in shanghai and 26 from tokyo has been studied for comparison. the results showed that the virulence of tubercle bacilli from shanghai was lower than that from tokyo. the difference was statistically significant (p less than 0.01). among the 44 cultures from shanghai high virulence accounted for 54.5%, low virulence 15.9% and middle virulence 29.5% whereas for the cultures from tokyo high v ... | 1989 | 2500258 |
| [current status of the laboratory diagnosis of tuberculous meningitis]. | | 1989 | 2500260 |
| detecting antimycobacterial antibodies. | | 1989 | 2500311 |
| identification of antigens of mycobacterium tuberculosis using human monoclonal antibodies. | pleural tuberculosis constitutes a human model of local protective immunity to mycobacterial infection as the disease is usually self-limited and recurrent pleurisy is rare. to identify potentially protective antigens of mycobacterium tuberculosis, 37 human pleural fluid b cell clones were established using ebv and their supernatants assayed by elisa and western blot for antibody reactivity with m. tuberculosis sonicate and culture filtrate. one antibody identified 29,000, 31,000, and 33,000 ban ... | 1989 | 2500454 |
| tuberculosis in the pediatric population of houston, texas. | from september 1984 to december 1987, the children's tuberculosis clinic in houston, tx, cared for 110 children with active tuberculosis. the median age was 24 months. approximately one half of the cases were in hispanic children, but one third were in black children. only 11% were foreign-born. diagnosis resulted from case contact investigation in 50% of cases, routine tuberculin screening in 6%, and evaluation of an ill child in 44%. intrathoracic disease alone was present in 77% of cases, and ... | 1989 | 2500637 |
| levels of antibody to defined antigens of mycobacterium tuberculosis in tuberculous meningitis. | the aim of the study was to develop an antibody immunoassay for tuberculous meningitis with a sensitivity greater than that of the initial bacterial smear while maintaining a 100% specificity. antibody titers to five purified antigens of mycobacterium tuberculosis were measured in cerebrospinal fluid by using an enzyme-linked immunosorbent assay technique. seventy-four patients with tuberculous meningitis (26 culture positive) were compared with 26 patients with purulent meningitis, 69 patients ... | 1989 | 2501348 |
| comparative evaluation of the diagnostic significance of circulating immune complexes and antibodies to phosphatidylinositomannosides in pulmonary tuberculosis by enzyme-linked immunosorbent assay. | an enzyme-linked immunosorbent assay was developed to detect circulating immune complexes (cic) and antibodies to phosphatidylinositomannosides (pim) of mycobacteria for the diagnosis of pulmonary tuberculosis. identification of pim in cic of smear and culture positive (spcp) cases revealed 88% sensitivity and antibodies to pim were detected in circulation in 94% cases. the smear negative and culture positive (sncp) group had 67% positivity with regards to pim in cic and 83% for antibodies to pi ... | 1989 | 2501636 |
| [diagnostic significance of determining specific antibodies of various isotypes in patients with pulmonary tuberculosis]. | the levels of antimycobacterial antibodies of three isotypes: igg, iga and igm were determined in serum of 153 patients with pulmonary tuberculosis and 136 healthy donors by the solid phase enzyme immunoassay with the sonicate as the antigen. a significant and highly reliable increase in the average levels of the antimycobacterial antibodies of the igg, iga and igm classes in the serum of the patients was detected as compared to the healthy persons. a significant increase (probability of 97.5 pe ... | 1989 | 2501777 |
| [an immunologic method for identification of m. tuberculosis and m. bovis (bcg) based on the use of monoclonal antibodies]. | monoclonal antibodies interacting with specific selectivity with human tubercle bacilli (monoclonal antibodies of the cell producing strain 62d) and bovine tubercle bacilli (bcg) (monoclonal antibodies of the cell producing strain 60d) were produced with hybridoma technology. a rapid, productive and safe procedure for identification of m. tuberculosis and m. bovis (bcg) was developed on the basis of solid phase enzyme immunoassay. the immunological procedure was compared with the biochemical (ni ... | 1989 | 2501778 |
| [taxonomic aspects of mycobacterium tuberculosis]. | | 1989 | 2501779 |
| [antitubercular agents. 43. a new group of potential antitubercular agents--oxalanilides and malonanilides]. | | 1989 | 2501802 |
| [comparison of the chemical structure and antimycobacterial action with particular reference to atypical strains. 3. oxalamidines, a new group of potential antitubercular agents]. | | 1989 | 2501803 |
| [antimycobacterial properties of phenothiazine type compounds]. | tests for antimycobacterial properties (mycobacterium tuberculosis h37 ra, middlebrook-7h9-broth) of the phenothiazines 1-11 are described. the growth inhibition of these compounds can be blocked by the addition of histamine. | 1989 | 2502103 |
| immunodiagnosis of tuberculosis: a review. | | 1989 | 2502129 |
| relapses after stopping chemotherapy for experimental tuberculosis in genetically resistant and susceptible strains of mice. | three strains of mice (swiss albinos, c57bl/6,c3h/ouj) were injected intravenously with 3.7 x 10(6) colony forming units (cfu) of m. tuberculosis, h37rv, sensitive and resistant to antibiotics (90% of bacilli sensitive, 9% resistant to streptomycin and 0.9% resistant to kanamycin). two weeks later, chemotherapy was started 6 days a week for a 6-month period with isoniazid (inh) and rifampicin (rmp). twenty mice of each strain were killed at the end of the chemotherapy and the others were kept wi ... | 1989 | 2502336 |
| bacteriological studies of tuberculosis in sidamo regional hospital. | all of the 104 strains isolated from tuberculosis patients in sidamo regional hospital were identified as m. tuberculosis. primary single drug resistance to isoniazid and streptomycin was represented by two strains in each case. four strains showed double drug resistance to the same drugs. none were resistant to thiacetazone, rifampicin and ethambutol. | 1989 | 2502390 |
| streptococcal cell wall arthritis. passive transfer of disease with a t cell line and crossreactivity of streptococcal cell wall antigens with mycobacterium tuberculosis. | primary lymph node cells derived from streptococcal cell wall arthritic rats or those derived from adjuvant arthritic rats proliferated in response to cell wall antigens derived from either streptococcal cell walls or those from m. tuberculosis. in addition, two t cell lines have been isolated from lymph nodes of rats during the chronic phase of streptococcal cell wall arthritis. these t cell lines transfered clinical disease to naive syngeneic irradiated recipients, and they proliferated in the ... | 1989 | 2502600 |
| tuberculous arthritis presenting as tophaceous gout. | a 73-year-old black man with chronic gout presented with a 1 1/2 year history of a swollen painful left wrist. two draining ulcers over the ventral aspect of his wrist and radiographic changes of the affected area suggested chronic gouty arthritis, but culture of the fluid from the wrist grew mycobacterium tuberculosis. this case extends the list of infectious agents producing septic arthritis in patients with gout to include mycobacterium tuberculosis. the need for culture evaluation of joint f ... | 1989 | 2502626 |
| simultaneous occurrence of mumps meningoencephalitis and tuberculosis meningitis. | | 1989 | 2502770 |
| [secondary multiple resistance during treatment of tuberculosis]. | a case of severe bilateral pulmonary tuberculosis is presented. during treatment, the patient developed resistance to a total of six drugs. the reasons were poor patient compliance and incorrect treatment. in order to avoid problems with resistant strains, it is important that the departments undertaking treatment have experience with the medicinal treatment and understanding of the problem of compliance. if it is not possible to carry out satisfactory treatment, the patient must be referred to ... | 1989 | 2502875 |
| [the mode of action of antimycobacterial benzylamines]. | the antimycobacterial effects of benzylamines are not related to an inhibition of aminoacyl-trna synthetases. | 1989 | 2502972 |
| lipoarabinomannan from mycobacterium tuberculosis induces the production of tumour necrosis factor from human and murine macrophages. | we show here that purified lipoarabinomannan (lam) from mycobacterium tuberculosis can cause the release of tumour necrosis factor (tnf) in vitro from human blood monocytes and activated mouse peritoneal macrophages, and the production of tnf in vivo in mice pretreated with propionibacterium acnes, with a potency comparable to that of lipopolysaccharide (lps) from gram negative bacteria. like lps, lam binds to polymyxin b. we confirmed that its activity was distinct from any contaminating lps an ... | 1989 | 2503277 |
| rapid diagnosis of tuberculous meningitis by elisa to detect mycobacterial antigen and antibody in the cerebrospinal fluid. | | 1989 | 2503566 |
| [effect of various peptides on the growth of mycobacterium tuberculosis]. | ten oligopeptides containing asparagine, glutamic acid, leucine or alanine on growth of bacillus tuberculosis were tested. the experiments were performed on as medium free of peptones. bacterial suspensions were inoculated and the number of colonies and rapidity of bacilli growth under an influence of peptides tested was compared. out of peptides studied and their different combinations the best turned to be combination of 0.01% glutathione +0.002% gly-asn + 0.0033% leu-gly. this combination all ... | 1989 | 2503665 |
| biochemical and antigenic characterization of the mycobacterium tuberculosis 71kd antigen, a member of the 70kd heat-shock protein family. | a 71 kilodalton antigen from mycobacterium tuberculosis is recognized by antibodies and by t lymphocytes during infection (britton et al., 1986a). partial sequence analysis indicates a relationship between this antigen and the highly conserved family of 70-kilodalton heat shock proteins (hsp70) (young et al., 1988). biochemical and serological characterization of the protein confirms its membership of the hsp70 gene family, and metabolic labelling demonstrates that it is a major component of the ... | 1989 | 2503672 |
| non-cultivable mycobacteria in ulcers of the skin. | in biopsies of 54 patients suffering from chronic dermatological lesions (mostly ulcers of the skin) acid-fast bacilli were found in 14. in these 14 cases in 4 were lesions caused by m. tuberculosis, in 1 the lesion was caused by m. avium-intracellulare, in 1 the lesion was caused by m. fortuitum and in 2 the lesions were caused by non-cultivable mycobacteria (feldmann-hershfield ulcer?). in 2 cases the cultures were heavily contaminated, and the diagnosis remained uncertain. in the remaining 4 ... | 1989 | 2503961 |
| identification of mycobacterial antigens for "elisa" serology in the diagnosis of leprosy and tuberculosis. | using an immunoblotting assay (imba), several immuno-crossreactive antigenic components (imcrac-myc) have been identified in the whole sonicates of m. bovis-bcg, and m. tuberculosis (mtb) and m. leprae (ml) whereby the sera of 100% lepromatous leprosy (l-lep) reacted to 29/33 kd doublet and that of 100% tuberculoid leprosy (t-lep) reacted to 64 kd bands. the antigens upon purification from mtb sonicates were used in a direct elisa to measure antibody isotypes in the sera from l-lep, t-lep, healt ... | 1989 | 2503964 |
| the use of pgl-tb 1 from m. tuberculosis in the serological diagnosis of tuberculous meningitis: a preliminary report. | | 1989 | 2503971 |
| antibody responses against mycobacterium tuberculosis and production of monoclonal antibodies in mice. | | 1989 | 2503986 |
| comparative studies of antigenic glycolipids of mycobacteria related to the leprosy bacillus. | the leprosy bacillus, mycobacterium leprae, is a member of a small group of mycobacteria comprising the species mycobacterium bovis, mycobacterium marinum, mycobacterium kansasii, mycobacterium tuberculosis, mycobacterium ulcerans and related taxa. this relationship is based on the similarity of the characteristic lipid types in the cell envelope. mycobacterium leprae produces a phenolic glycolipid antigen which is species specific. this communication reports a comparison of the specificity of t ... | 1989 | 2504005 |
| detection of mycobacteria in infected tissue. | | 1989 | 2504007 |
| evaluation of a phenolglycolipid antigen (pgl-tb 1) from m. tuberculosis in the serodiagnosis of tuberculosis: comparison with ppd antigen. | sera from 38 tuberculous patients and 62 healthy controls (31 ppd skin test positive and 31 negative) were assayed, by enzyme-linked immunosorbent assay (elisa), to test the activity of igg and igm antibodies against purified protein derivative (ppd) antigen and a phenolglycolipid antigen (plg-tb 1) isolated and purified from mycobacterium tuberculosis strain canetti. using ppd antigen, the sensitivity and specificity were respectively, 50 and 93.5% for igg and 71.1 and 59.7% for igm antibody ac ... | 1989 | 2504014 |
| serological specificity of mycobacterium tuberculosis glycolipids. | six glycolipid fractions can be extracted from lipid crude extracts of canetti type strains of m. tuberculosis. among these fractions are two phenolglycolipids. the major component is a triglycosyl phenolphthiocerol dimycocerosate (pgl-tb 1) and the second is a monoglycosyl diacyl phenolphthiocerol identical to mycoside b of m. bovis. similar glycolipid compounds can also be found in wild strains of m. tuberculosis recently isolated from tuberculous patients. one of these compounds has been iden ... | 1989 | 2504016 |
| mixed mycobacterial cultures--occurrence in the clinical laboratory. | during a 2-year period we observed at our institution 22 mixed mycobacterial cultures among a total of 991, representing 20 patients. we found no evidence of double infection. mixed cultures frequently contained saprophytic organisms. our experiments have shown that subculturing, especially into liquid media, can significantly change the relative quantities of cfu's present in mixed cultures. we believe that the use of agar media is useful for the recognition of mixed cultures. | 1989 | 2504179 |
| mycobacterium tuberculosis cervical adenitis. diagnosis and management. | cervical adenopathy as the sole presentation of tuberculosis is seen infrequently in the pediatric age group. despite modern treatment and public health measures, tuberculous cervical adenitis persists, and its prompt diagnosis and treatment are important from both a clinical and preventive health perspective. the authors report five cases of children with ages ranging from 8 to 17 years, in whom cervical adenitis was the only presentation of infection with mycobacterium tuberculosis. the clinic ... | 1989 | 2504530 |
| direct identification of mycobacterium tuberculosis, mycobacterium avium, and mycobacterium intracellulare from amplified primary cultures in bactec media using dna probes. | dna probes (gen-probe, san diego, calif.) directed at the mycobacterium tuberculosis complex and mycobacterium avium-m. intracellulare complex were used to identify acid-fast bacilli directly from specimens grown in bactec 12b bottles (becton dickinson and co., towson, md.). clinical specimens were inoculated directly or after decontamination into a bactec 12b bottle, middlebrook 7h11 agar, and lowenstein-jensen medium. conventional media were incubated at 37 degrees c in 5% co2 and examined wee ... | 1989 | 2504770 |
| the importance of penetration of antimicrobial agents into cells. | phagocytes may shelter intracellular pathogens from the otherwise lethal effects of many antibiotics. research in conditions such as chronic granulomatous disease, tuberculosis, legionellosis, and experimental staphylococcal infection underscores the principle that an antibiotic must enter the cell in order to be active against an intracellular microorganism. demonstrating entry does not guarantee activity, however. the microenvironment and intracellular distribution of the pathogen and antimicr ... | 1989 | 2504811 |
| preparation and properties of monoclonal antibodies against mycobacterium tuberculosis. | the above mentioned monoclonal antibodies of idiotype igg 3/kappa against tmc 120 and tmc 107 strains of m. tuberculosis were prepared by fusion of mouse myeloma line fo cells with splenocytes balb/c of mice immunized with complex antigens. these were prepared from the bacterial mass previously inactivated with gamma-rays radiation, and applied without mycobacterial adjuvant agent. species specificity of both monoclonal antibodies was determined by elisa testing and dot blot test. the potential ... | 1989 | 2504816 |
| [primary resistance to antitubercular drugs]. | | 1989 | 2505005 |
| identification of two unknown reading frames in synechococcus 6301 as homologues of the 10k and 65k antigen genes of mycobacterium tuberculosis and related heat shock genes in e. coli and coxiella burnetii. | | 1989 | 2505234 |