| effect of protein-modifying reagents on water-soluble and membrane bound ovarian lh/hcg receptor. | preincubation of corpora lutea membrane and follicular fluid water-soluble receptor with protein-modifying reagents suggests that sulfhydryl, histidyl and tyrosyl groups are involved in the interaction of hcg with the receptor. | 1987 | 3609166 |
| serological studies of british leptospiral isolates of the sejroe serogroup. iii. the distribution of leptospires of the sejroe serogroup in the british isles. | some 94 strains of leptospires belonging to the sejroe serogroup isolated in the british isles were identified to the serovar level using specific factor sera. seventy strains were identified as leptospira interrogans serovar hardjo, 66 from cattle, 2 from pigs and 1 each from a sheep foetus and a human. twenty-four strains were identified as l. interrogans serovar saxkoebing, most strains were isolated from either wood mice, bank or field voles but strains were also isolated from badgers, a fox ... | 1987 | 3609169 |
| effects of hypercholesterolemia on the microsomal membrane fluidity of intimal-medial versus medial layers of swine aorta: implications for the pathogenesis of vasospasm. | the hypothesis was examined that hypercholesterolemia induces a decrease in arterial microsomal membrane fluidity. to investigate this hypothesis, the fluorescence anisotropy (r) of 1,6-diphenylhexa-1,3,5-triene was measured in aortic microsomes isolated from the intimal-medial (im) and medial (m) layers of swine thoracic aortas. after 10 weeks of feeding a high fat (10% lard) diet, serum cholesterol increased 2.3-fold compared to 3.6-fold in pigs fed a similar diet supplemented with 2% choleste ... | 1987 | 3609245 |
| [effect of piracetam on rat reproductive function and the sexual development of their progeny]. | administration of piracetam to infantile rats in a daily dose of 100 mg/kg for 10 days caused acceleration of sexual maturation of females and a slight delay of maturation of males. antenatal administration of the same dose of piracetam exerted a depressive action on the development of the organs of the reproductive system in males from the offspring with a simultaneous increase in the pituitary gonadotropic activity. | 1987 | 3609283 |
| [contractile properties of isolated lung tissue preparations]. | | 1987 | 3609372 |
| large scale multiplication of bacillus thuringiensis h. 14 asporogenic mutants & b. sphaericus strains for mosquito control. | | 1987 | 3610270 |
| thermally induced disulfide bond exchanges in human ige. | the thermally induced changes in human ige was investigated using sodium dodecyl sulfate-polyacrylamide gel electrophoresis. when the ige (1 mg/ml) was heated at 56 degrees c for several hours in the absence of sodium dodecyl sulfate, it gave aggregated forms that could be reduced completely to heavy and light chains with dithiothreitol. on the other hand, in the presence of sodium dodecyl sulfate (0.1%), the ige was changed by heating to five components with small mol. wts dependent on temperat ... | 1987 | 3614214 |
| detecting in vitro anthelmintic effects with a micromotility meter. | an in vitro target parasite anthelmintic assay utilizing a micromotility meter has been developed and validated. haemonchus contortus, an economically important ruminant helminth with worldwide distribution, was the parasite used in the model. four commercially available ruminant anthelmintics (albendazole, ivermectin, levamisole hydrochloride and coumaphos) were initially evaluated at concentrations of 200, 150, 100 and 50 micrograms ml-1. all four significantly affected helminth motor activity ... | 1987 | 3617429 |
| differences in the stimulation of repair replication by 3-aminobenzamide in lymphoblastoid cells damaged by methylmethanesulfonate or ultraviolet light. | human lymphoblastoid cells damaged by u.v. light accumulated dna breaks in the presence of cytosine arabinoside and hydroxyurea at a frequency similar to that of cells damaged by methylmethanesulfonate. 3-aminobenzamide (1 mm) reduced the net strand-break frequency detected after either kind of damage. repair replication, however, was stimulated only in methylmethanesulfonate-damaged cells. this stimulation is therefore not related directly to the dna strand-break frequencies and concomitant pol ... | 1987 | 3621467 |
| effect of apoe on triglyceride emulsion interaction with hepatocyte and hepatoma g2 cells. | the uptake and internalization of a triglyceride emulsion by rat hepatocytes in culture less than 24 hr was either inhibited or uninfluenced by apoe. apoe significantly increased the uptake of these emulsions in later cultures. specific low density lipoprotein (ldl) binding was similar for hepatocyte monolayers prior to and after 24 hr. rat hepatocytes in culture for 2 days, which were treated with collagenase, detached and then replated within 1 hr and were apoe-responsive in 2 hr. heparin inhi ... | 1987 | 3625037 |
| protein binding of glycopeptide antibiotics with diverse physical-chemical properties in mouse, rat, and human serum. | in previous studies of the pharmacokinetics and urinary excretion of nine glycopeptides with diverse isoelectric points (pi), as pi decreases, the total systemic and renal clearance, urinary recovery, and volume of distribution decrease, whereas the half-life increases. with glycopeptides of similar pi, clearance decreases and half-life increases with increasing lipophilicity. the present study examines the serum protein binding of these glycopeptide antibiotics in mouse, rat, and human serum an ... | 1987 | 3625479 |
| efficacy of single-agent therapy with azlocillin, ticarcillin, and amikacin and beta-lactam/amikacin combinations for treatment of pseudomonas aeruginosa bacteremia in granulocytopenic rats. | the efficacy of azlocillin, ticarcillin, and amikacin as single agents and the penicillin/amikacin combinations for treatment of pseudomonas aeruginosa bacteremia during cyclophosphamide-induced severe neutropenia in a rat model were assessed. equivalent antibiotic dosing was based on the time rat serum antibiotic levels were above the minimal bactericidal concentration for the challenge organism. antibiotic therapy was administered for 62 hours after bacterial challenge. antimicrobial efficacy ... | 1986 | 3636060 |
| treatment of cerebrospinal fluid with formalin from patients infected with human immunodeficiency virus before diagnostic microscopy. | | 1987 | 3643930 |
| determination of mevinolin and mevinolinic acid in plasma and bile by reversed-phase high-performance liquid chromatography. | | 1986 | 3644824 |
| the growth rate of sensory nerve fibres in the mammalian embryo. | i have used a novel quantitative electron microscopic method to determine the rate at which nerve fibres grow towards their targets during development. the rate of recruitment of nerve fibres to the maxillary nerve of the mouse embryo was determined by counting the number of axon profiles in the nerve sectioned close to its emergence from the trigeminal ganglion at closely staged intervals throughout its early development. the rate of change of fibre number with distance along this nerve was det ... | 1987 | 3652972 |
| expression of liver fluke antigens by mouse cells. | | 1987 | 3654047 |
| expression of liver fluke antigens by mouse cells. | | 1987 | 3654047 |
| [3-dimensional reentry in paroxysmal ventricular tachycardias: the results of electrophysiological mapping]. | the mechanism of sustained ventricular tachycardias with a focal type of activation initiated by an extrastimulus was studied in isolated rabbit heart ventricle preparations by endocardial and epicardial mapping. sixty-four electrograms were simultaneously recorded using unipolar electrodes (32 on each side of the preparation). the electrodes were spaced 3 mm apart. data recording and processing were computerized. sustained (greater than 0.5 min) tachycardias of a focal type were registered in 6 ... | 1987 | 3656897 |
| influence of nifedipine on arterial baroreflex modulation of heart rate control during dynamic increases in arterial pressure: studies in normal man. | studies in animals have demonstrated that calcium channel blocking agents exert important influences on autonomic mechanisms in addition to their direct vascular effects. previous studies in our laboratory showed that clinical doses of nifedipine sensitized baroreceptor-mediated control of peripheral vascular resistance in normal human subjects. however, baroreflex control of vascular tone does not necessarily imply parallel control of heart rate. a series of experiments was therefore performed ... | 1987 | 3661368 |
| [volatile anesthetics and n-alcohols inhibit the uptake of noradrenaline in pheochromocytoma cells]. | studies with perfused animal organs did not show a significant inhibition of the norepinephrine uptake by volatile anesthetics however, the uptake of norepinephrine into isolated chromaffin granules proved to be halothane-sensitive , and there is indirect evidence for an inhibition of norepinephrine uptake by halothane in the dog saphenous vein. so far no literature is available on norepinephrine uptake into cultured cells in the presence of volatile anesthetics. the rat pheochromocytoma cell li ... | 1987 | 3661947 |
| vasculitis and renal disease in nail-patella syndrome: case report and literature review. | a 57 year old man with nail-patella syndrome (nps) and associated renal disease is described who developed an inflammatory polyarthropathy and polyarteritis-like vasculitis. vasculitis and serum complement abnormalities have not previously been reported in nps. nps is a rare autosomal dominant connective tissue disorder affecting both mesenchymal and ectodermal tissue. the condition is reviewed with particular reference to its renal pathology, including the distinctive electron microscopic (em) ... | 1987 | 3662646 |
| immediate and long-term effects of opiate antagonists on postictal behaviour following amygdala kindling in the rat. | male wistar rats implanted with bipolar electrodes in the amygdaloid complex were kindled. subcutaneous injection of naloxone or naltrexone in low doses--0.12 and 0.24 mg/kg, respectively--dramatically reduced the postictal behavioural depression (bd) at 10 or 60 min. remarkably, the bd was still reduced one day later. it would appear that the brain mechanisms involved in postictal bd use mu-receptors since bd is quite sensitive to low doses of the preferential antagonists naloxone and naltrexon ... | 1987 | 3666034 |
| liquid chromatographic analysis of alizapride and metoclopramide in human plasma and urine using solid-phase extraction. | a universal rapid, sensitive and selective high-performance liquid chromatographic method with uv detection at 230 nm has been developed for the determination of benzamide drugs in human plasma and urine. sample pretreatment is carried out using solid-phase extraction columns, resulting in very high extraction recoveries of the compounds investigated (alizapride, metoclopramide, alpiropride, amisulpride). the detector response is linear from 25 to 10,000 ng/ml, and the detection limit is 3 ng/ml ... | 1987 | 3667781 |
| infection following a dog bite. | | 1987 | 3668208 |
| effect of dietary glycine on methionine metabolism in rats fed a high-methionine diet. | the alleviation mechanism of methionine toxicity by dietary glycine was investigated in weanling rats fed a high-methionine diet. when rats were fed a 10% casein diet containing 2% methionine, the activities of methionine adenosyltransferase, cystathionine beta-synthase, and cystathionine gamma-lyase, which participate in the methionine metabolism in the transsulfuration pathway, were significantly enhanced. but the addition of 2% glycine to the high methionine diet did not cause further increas ... | 1987 | 3668700 |
| properties of equine anti-lipopolysaccharide hyperimmune plasma: binding to lipopolysaccharide and bactericidal activity against gram-negative bacteria. | anti-lipopolysaccharide equine hyperimmune plasma (anti-lps), which has been used successfully to treat lps (endotoxin)-mediated disorders, has been further characterised. igg present in anti-lps had the highest affinity for lps prepared from salmonella typhimurium, s. typhi, s. abortus equi and shigella flexneri and intermediate affinity for escherichia coli o55:b5, e. coli o127:b8 and s. enteritidis. anti-lps destroyed by means of complement activation a wide range of gram-negative bacteria, i ... | 1987 | 3669052 |
| conservative management of muscle-infiltrating bladder cancer: prospective experience. | between may 1979 and july 1983, 217 consecutive patients with documented primary bladder tumors invading muscle were evaluated to determine the fate of patients with conservatively treated muscle-infiltrating bladder cancer. the disease was re-staged by urine cytology, bimanual examination with the patient under anesthesia and transurethral biopsy or resection. of the 217 patients 172 underwent total or partial cystectomy and 45 (21 per cent, 37 with stage t2, 7 with stage t3a and 1 with stage t ... | 1987 | 3669160 |
| conservative management of muscle-infiltrating bladder cancer: prospective experience. | between may 1979 and july 1983, 217 consecutive patients with documented primary bladder tumors invading muscle were evaluated to determine the fate of patients with conservatively treated muscle-infiltrating bladder cancer. the disease was re-staged by urine cytology, bimanual examination with the patient under anesthesia and transurethral biopsy or resection. of the 217 patients 172 underwent total or partial cystectomy and 45 (21 per cent, 37 with stage t2, 7 with stage t3a and 1 with stage t ... | 1987 | 3669160 |
| [role of hippocampal acetylcholine-sensitive neurons in different stages of nictitating membrane conditioning of rabbits]. | | 1987 | 3672137 |
| [an example of bvd infection from practice]. | | 1987 | 3672471 |
| [biopharmaceutical evaluation of the rectal administration of mefenamic acid. iv. biological availability of mefenamic acid on the base of polyoxyethylene glycol with the addition of polysorbates]. | | 1987 | 3673627 |
| measurement of branched-chain ketoacids in plasma by high-performance liquid chromatography. | branched-chain ketoacids were isolated from plasma or serum samples by acidification, passage through a cationic exchange resin, ether extraction, and extraction of the ether layer with phosphate buffer. the recovery of 2-[1-14c]ketoisocaproate taken through these procedures averaged 95 +/- 3%. branched-chain ketoacids were measured by high-performance liquid chromatography using a single mobile phase (sodium phosphate:acetonitrile). in normal human subjects, mean +/- sd fasting levels of 2-keto ... | 1987 | 3674376 |
| sample preparation for gas chromatography with electron capture detection: determination of total and free thyroxin in serum. | relatively clean gas chromatography with electron capture detection (gc-ecd) chromatograms are obtained for both total and free thyroxin (t4) in serum by improving sample preparation. this is based on establishing a sequence of steps that cumulatively overcome two classes of interference: those present in the initial sample and those introduced by the procedure. the main source for the latter contaminants is the derivatization step, a problem that was largely overcome by employing hplc after thi ... | 1987 | 3674391 |
| prevalence of intestinal parasitic infections in riyadh district, saudi arabia. | a total of 23,516 stool specimens collected from patients attending three medical centres in riyadh, saudi arabia, were examined for intestinal parasites. of these 5737 (24.4%) were positive. entamoeba histolytica (8.8%) and giardia lamblia (6.3%) were the commonest parasites found, and in the age group of one to 15 years. giardia was found in 14.8% of males and 11.9% of females. other intestinal parasites present included ascaris lumbricoides, trichuris trichiura, schistosoma mansoni, hymeolepi ... | 1986 | 3675035 |
| cerebrovascular effects of isoflurane in man. intracranial pressure and middle cerebral artery flow velocity. | twelve hydrocephalic patients undergoing hydrodynamic studies and shunt procedures during general anaesthesia were subjected to short term observations of the cerebrovascular effects of isoflurane. intraventricular pressure (ivp), arterial pressure (ap); cerebral perfusion pressure (cpp = ap-ivp), end-tidal pco2, cerebral function monitor (cfm) activity and middle cerebral artery (mca) flow velocity were measured. introduction of 1% isoflurane for a 10-min period did not cause significant change ... | 1987 | 3676050 |
| human monoclonal cryoimmunoglobulins. ii. comparative studies on the carbohydrate contents and compositions of cryoglobulins and myeloma proteins. | the carbohydrate contents and compositions of cryoglobulins and myeloma proteins were comparatively investigated by gas-liquid chromatographic and colorimetric analyses. human monoclonal cryoglobulins, jir (igg3 kappa), wat (igg3 lambda), fji (igm kappa), and human myeloma proteins, har (iggl kappa) and kob (iga kappa) were used for the present experiments. the results obtained were that the carbohydrate contents of jir and wat were 2.7 and 4.4% in weight basis; the n-acetyl glucosamine of wat w ... | 1987 | 3685711 |
| distribution of immunoreactive inhibin like material in different regions of human prostate. | distribution of immunoreactive inhibin material (ir-ilm) in different regions of human prostate was studied by using specific radio-immunoassay. mean ir-ilm concentrations in the central, peripheral and periurethral zones were 27.3 +/- 9.3, 2.4 +/- 0.7 and 3.0 +/- 1.5 micrograms/mg protein respectively (p central vs periurethral and peripheral less than 0.05). | 1987 | 3688474 |
| effects of changed lipid composition on responses of liposomes to various odorants: possible mechanism of odor discrimination. | in a previous paper [nomura, t., & kurihara, k. (1987) biochemistry (preceding paper in this issue)], we showed that azolectin liposomes are depolarized by various odorants and there is a good correlation between the responses in the liposomes and the frog or porcine olfactory responses. in this study, we examined effects of changed lipid composition on responses of liposomes to various odorants. the membrane potential changes in response to odorants were monitored with the fluorescent dye 3,3'- ... | 1987 | 3689768 |
| effects of cold stress on brain regional calcium content in rats and mice. | the calcium levels of striatum, cerebellum, cortex, medulla and diencephalon from rat brain were significantly reduced after 30 minutes of cold exposure at 8 degrees c even though body temperatures were no different from pre-cold exposure values. calcium content remained significantly depressed in striatum, cerebellum and diencephalon after 2 hours of cold exposure, at which time body temperatures were significantly depressed. a similar effect of short-term cold stress on calcium content in thes ... | 1987 | 3690357 |
| [clinico-anatomical and immunomicrobiological aspects of infection caused by gram-negative bacteria with lung lesions in children]. | | 1987 | 3696845 |
| response of dog tracheal stretch receptors to high frequency oscillations. | in eight dogs, anaesthetized and spontaneously breathing, we recorded from thin vagal filaments action potentials from eight slowly adapting receptors of the extrathoracic trachea. the extrathoracic trachea was cut longitudinally and the cut-ends of the cartilage corresponding to the site of the receptor in the trachealis muscle were stretched sinusoidally at frequencies from 0.5 to 39.5 hz. the number of spikes per cycle decreased with increasing frequency. at the highest frequency (40 hz), str ... | 1986 | 3697539 |
| spinal cord serotonin release and raised blood pressure after brainstem kainic acid injection. | the recently developed technique of in vivo dialysis has permitted us to make direct measurements of serotonin release in a specific region of the spinal cord and to relate this to changes in blood pressure elicited by chemical stimulation of the brainstem. in the present experiments we have shown that chemical stimulation of bulbospinal neurons in the region of the b3 cell group in the ventromedial medulla, causes an increase in the release of serotonin in the thoracic spinal cord and that this ... | 1986 | 3697690 |
| evidence for the presence of a novel biosynthetic pathway for norspermidine in vibrio. | enzymatic studies of the cell extracts of vibrio alginolyticus and v. parahaemolyticus provided evidence that there exists a novel biosynthetic pathway for norspermidine (nh2(ch2)3nh(ch2)3nh2), a major polyamine species. in this pathway, the schiff base formed between aspartic beta-semialdehyde and 1,3-diaminopropane is first reduced by a nadph-dependent enzyme to yield "carboxynorspermidine" (nh2(ch2)3nh(ch2)2ch(nh2)cooh), which is in turn decarboxylated by a pyridoxal phosphate dependent enzym ... | 1986 | 3697846 |
| rate of incorporation of [3h]thymidine in various tissues of the mouse. a basis for the evaluation of genotoxic effects of chemicals. | [3h]thymidine has been extensively used as a selective precursor to dna in studies on the kinetics of cell proliferation. we have become interested in measuring early inhibition of the dna synthesis in various organs of intact animals for detecting genotoxic properties of chemicals. such experiments should, for convenience and to achieve a large capacity, be performed in the simplest way possible. the present paper deals with some practical aspects on the use of [3h]thymidine in vivo. [6-3h]thym ... | 1986 | 3698076 |
| [cytogenetic activity of metabolites of pesticides representing different classes of chemical compounds]. | the cytogenetic activity of some substances formed in agricultural plants during metabolism of pesticides of four classes of chemical compounds was studied in the culture of human peripheric blood lymphocytes. metabolites were shown either to have mutagenic properties similar to those of the initial compounds (ziramtetramethylthiourea, both being mutagens; captan-phthalimide, both possessing no cytogenetic activity) or to be considerably transformed in comparison with them as a result of deactiv ... | 1986 | 3705167 |
| somatotopic organization of motoneurons innervating the pronators, carpal and digital flexors and forepaw muscles in the dog: a retrograde horseradish peroxidase study. | central distribution of motor neurons innervating the individual forearm and forepaw muscle through the median and ulnar nerves was studied in the dog using the retrograde horseradish peroxidase (hrp) method. all hrp-labeled cells were seen ipsilaterally in restricted parts within the dorsolateral (dl) and retrodorsolateral nucleus (rdl) of the ventral horn at levels from the cranial tip of c7 to the cranial third of t2. the results showed that motoneurons situated more cranially supply the more ... | 1986 | 3708348 |
| increased collateralization of the cerebellothalamic pathway following neonatal hemicerebellectomy. | the postlesional reorganization of the cerebellothalamic cells was here studied using a multiple retrograde tracing technique. to this purpose, the cerebellothalamic cell population was investigated in 3 groups of adult rats: (a) cases hemicerebellectomized at birth; (b) cases hemicerebellectomized in adulthood; and (c) control unlesioned ones. in all of the groups the fluorescent tracers diamidino yellow and fast blue were injected bilaterally in the thalamus and the retrograde labeling obtaine ... | 1986 | 3708349 |
| the effect of amiodarone on thyroxine kinetics. | amiodarone was given by daily intraperitoneal injection (10 mg/kg body weight) to a group of 11 new zealand white rabbits. over 6 weeks there were significant increases (p less than 0.01) in plasma total t4 concentration (43 +/- 8 to 60 +/- 13 nmol/l; mean +/- 1 sd) and rt3 concentration (0.12 +/- 0.06 to 0.31 +/- 0.16 nmol/l) and a significant fall in total plasma t3 (2.3 +/- 0.3 to 1.7 +/- 0.2 nmol/l). the plasma clearance of t4 in rabbits treated with amiodarone for 6 weeks was significantly ... | 1986 | 3708866 |
| monoclonal antibodies to human eosinophil plasma membrane antigens enhance the secretion of eosinophil cationic protein. | this study was done to examine the nature of the membrane constituents involved in the secretion of eosinophil cationic protein (ecp) from human blood eosinophils. three mouse monoclonal antibodies were used, which showed greater binding to membrane antigens on activated, and light density eosinophils from patients with an eosinophilia, than on nonactivated or normal density eosinophils. all three antibodies (eon4, eon5 & eon6) stimulated normal density human eosinophils to secrete ecp, either a ... | 1986 | 3708911 |
| [effect of aevite and riboflavin on the electrolyte balance in the wall of the hepatic blood vessels in experimental toxic hepatitis]. | the effects of aevite and riboflavin on electrolyte balance in the vessel wall were studied in toxic hepatitis in the cat. significant changes in the contents of na+, k+, ca2+ and mg2+ ions in the hepatic blood vessels were found in all periods of observation. the treatment of toxic hepatitis with aevite and riboflavin results in normalization of electrolyte balance of the vessel wall. | 1986 | 3709781 |
| [hemodynamic correlates of immobilization stress in the rat]. | changes of the systemic and regional hemodynamics were studied in alert wistar rats during their immobilization. cardiac output and regional blood flows were measured with microsphere technique. the stress evoked 12% blood pressure increase, 30% cardiac output increase and 10% total peripheral vascular resistance decrease. blood flow was increased in skeletal muscles, in the heart and adrenal glands, whereas in the kidney and small intestine blood flow decreased. | 1986 | 3709865 |
| low-level lead exposure affects latent learning in the rat. | neonatal rats were administered intragastrically either lead acetate (50 mg pb/kg-bw) or an equal molar solution of sodium acetate at days 6, 9, 12, 15, and 18 postpartum. at 33 days of age, each animal began a training sequence to develop maze running skills. animals within each treatment group, i.e., lead exposed (pb), control vehicle (cv), and control nonhandled (cnh) were assigned randomly to either latent learning or open-field testing groups. the former individually explored a symmetrical ... | 1986 | 3713959 |
| reversible electroencephalogram changes associated with administration of baclofen in a quadriplegic patient: case report. | we present the case of a spastic quadriplegic who developed mental symptoms which resolved when his baclofen was discontinued. of interest was the presence of eeg abnormalities similar to those described in cats receiving this drug. these abnormalities, previously unreported in humans, resolved upon discontinuing baclofen therapy. | 1986 | 3714292 |
| leptospira hardjo agalactia in sheep. | | 1986 | 3716127 |
| multifocal eosinophilic granuloma. response of a patient to etoposide. | a 60-year-old man with a history of diabetes insipidus presented with a left groin mass, leg edema, and retroperitoneal adenopathy. biopsy results of the involved lymph nodes were typical of eosinophilic granuloma. the patient was treated with etoposide and prednisone and had a complete regression of his lymphadenopathy and edema. this response suggests that etoposide may be a useful agent in the management of histiocytosis x. | 1986 | 3719552 |
| tumor metastasis-associated heparanase (heparan sulfate endoglycosidase) activity in human melanoma cells. | we discovered previously a tumor metastasis-associated heparan sulfate (hs)-degrading endoglycosidase in mouse melanoma cells that is a unique endo-beta-glucuronidase (heparanase) capable of specifically cleaving hs at intrachain sites (nakajima et al., j. biol. chem., 259 (1984) 2283. using unmodified- and chemically modified-hs and heparin substrates we demonstrate that human hs939 and mouse b16 melanoma cells possess a hs-degrading endoglycosidase of similar specificity. melanoma heparanase s ... | 1986 | 3719568 |
| detection of extravasation of antineoplastic drugs by microwave radiometry. | microwave radiometry may be used as a non-invasive technique for subcutaneous thermal sensing. this technique was capable of demonstrating extravasation of small volumes of fluids, including the antineoplastic agent adriamycin, when these fluids were administered at room temperature in dogs. the rate of temperature drop upon extravasation was proportional to the flow rate. it is feasible that microwave radiometry may be useful as an alarm system to reduce serious complications which often accomp ... | 1986 | 3719569 |
| serum immune response to shigella protein antigens in rhesus monkeys and humans infected with shigella spp. | the serum antibody response to proteins encoded by the virulence-associated plasmid of shigella flexneri was determined in monkeys challenged with virulent s. flexneri serotype 2a. with water-extractable antigen in an enzyme-linked immunosorbent assay, a significant increase in antibody titer against proteins from a plasmid-carrying, virulent strain of s. flexneri serotype 5 could be demonstrated in convalescent sera. there were minimal antibody titers against proteins from an avirulent (plasmid ... | 1986 | 3721580 |
| effect of thyroid hormones on plasma protein concentrations in man. | plasma concentrations of proteins secreted by the liver (prealbumin, haptoglobin, transferrin, ceruloplasmin, alpha 1-antitrypsin, antithrombin iii, and t4-binding globulin) and proteins mainly derived from endothelium [fibronectin, angiotensin-converting enzyme (ace), and factor viii-related antigen (f viii r:ag)] were measured in 27 hyperthyroid and 30 normal women. significantly increased plasma concentrations (p less than 0.01) of endothelium-associated proteins, including fibronectin, ace, ... | 1986 | 3722331 |
| salmonella hepatitis: detection of salmonella antigens in the liver of patients with typhoid fever. | | 1986 | 3722872 |
| renal function in the fetal lamb: a chronic model to study physiological effects of ureteral ligation and deligation. | between 53 and 98 days of gestation 11 fetal lambs were subjected to unilateral ureteral ligation. in 8 fetuses re-exploration was performed 35 to 41 days after ligation. chronic catheterization of the obstructed kidney, bladder and fetal vascular system was used for analysis of physiological data. average creatinine clearance was 1.35 +/- 0.067 ml. per minute in the control kidney and 0.226 +/- 0.057 ml. per minute in the obstructed kidney after diversion. free water clearance, osmolar clearanc ... | 1986 | 3723669 |
| [clostridium difficile and its significance as an intestinal pathogenic bacterium]. | | 1986 | 3725396 |
| [associated vaccination of pigs against swine fever, erysipelas and aujeszky's disease]. | tested was the simultaneous vaccination of pigs against swine fever, swine erysipelas, and aujeszky's disease. parallel to this experiment a second group of test pigs were treated with each of the three vaccines at twenty-day intervals. the effect of vaccination was assessed through clinical observations with the test groups and by challenging part of the animals with virulent strains of the swine fever and erysipelas agents. it was found that the successive application of the three vaccines at ... | 1986 | 3727391 |
| oxidative injury to the vascular endothelium. | in recent years, increasing clinical and experimental data have provided compelling evidence that neutrophil-derived oxygen radicals and their metabolites are important mediators of vascular endothelial injury. in this review, attention is directed toward in vitro, ex vivo, and in vivo experimental studies contributing to current understanding of mechanisms of oxyradical mediated endothelial damage. although these studies may have broad significance, they appear to have particular relevance to t ... | 1986 | 3728559 |
| low-molecular-weight proteinase inhibitor of human epidermis inhibiting papain and trypsin activity. | a low-molecular-weight proteinase inhibitor was isolated from human epidermal extracts by means of ultrafiltration, gel filtration and ion-exchange chromatography. on polyacrylamide gel electrophoresis (page), the isolated fraction exhibited a single band of activity at ph 9.4, and inhibitory activity against papain and trypsin was detected in this band. using sodium-dodecyl-sulphate page, the molecular mass of the inhibitor was estimated to be 2,200 daltons. | 1986 | 3729540 |
| [induction of cytochrome p-450 and b5 in hepatocyte subpopulations with phenobarbital]. | hepatocytes isolated from intact and phenobarbital-treated rats were separated by ficoll discontinuous density gradient into five subpopulations. about 85% of the total cell number sedimented within the range of 1.044 to 1.126 g x cm-3. in intact rats, heavy hepatocytes contained about twice as much cytochrome p-450 and about 3 times as much cytochrome b5 as light cell population. phenobarbital induced cytochrome p-450 in all subpopulations, with a more pronounced increase observed in light hepa ... | 1986 | 3730555 |
| [induction of cytochrome p-450 and b5 in hepatocyte subpopulations with phenobarbital]. | hepatocytes isolated from intact and phenobarbital-treated rats were separated by ficoll discontinuous density gradient into five subpopulations. about 85% of the total cell number sedimented within the range of 1.044 to 1.126 g x cm-3. in intact rats, heavy hepatocytes contained about twice as much cytochrome p-450 and about 3 times as much cytochrome b5 as light cell population. phenobarbital induced cytochrome p-450 in all subpopulations, with a more pronounced increase observed in light hepa ... | 1986 | 3730555 |
| vacant postsynaptic densities on supraoptic dendrites of adult rats diminish in number with chronic stimuli. | in earlier ultrastructural studies of the supraoptic nucleus in adult rats we noted "free" and incompletely covered postsynaptic densities (collectively referred to here as vacant postsynaptic densities) on dendritic shafts. free postsynaptic densities have been reported in other parts of the central nervous system of normal rodents. we investigated the possibility that physiological activation of the supraoptic cells, which produces changes in many aspects of their morphology, would alter the i ... | 1986 | 3731249 |
| structure and x-ray amino acid sequence of a bacteriochlorophyll a protein from prosthecochloris aestuarii refined at 1.9 a resolution. | the structure of the water-soluble bacteriochlorophyll a protein (bchl protein) from the green photosynthetic bacterium prosthecochloris aestuarii has been refined at 1.9 a resolution to a crystallographic residual of 18.9%. the refinement was carried out without knowledge of the amino acid sequence and has led to an "x-ray sequence". the structure consists of seven bchl molecules enclosed within a protein "bag" and the refinement supports the general conformation of the molecule described previ ... | 1986 | 3735428 |
| age related heterogeneity of lipopigments in human sympathetic ganglia. | the histochemical, autofluorescence and morphological characteristics of lipopigments accumulating with age in the human sympathetic neurons were studied. the spectral characteristics of the lipopigment autofluorescence change with age suggesting the accumulation of additional components in the residual bodies. this component is electron microscopically highly osmiophilic and stains also with silver staining. these changes may indicate the gradual melanization of the lipopigments due to auto-oxi ... | 1986 | 3736128 |
| [a method for registration of stomach movement of rabbits in chronic experiments]. | | 1986 | 3738532 |
| fragile papilloma (papillary adenoma) of the gallbladder. a cause of recurrent biliary colic. | this case study of a young man with a papillary adenoma of the gallbladder documents the potential for benign tumors of the gallbladder to present with symptoms of biliary colic. the case illustrates the mechanism by which free-floating, detached, tumor particles cause intermittent obstruction of the cystic duct, and it presents the attendant symptoms. | 1986 | 3743976 |
| comparison of the surface morphology of adult schistosoma japonicum (chinese, philippine and indonesian strains) by scanning electron microscopy. | | 1986 | 3744663 |
| [euphorbia and high chapparall--hazardous pot plants in fashion]. | | 1986 | 3747661 |
| [campylobacter pyloridis--a cause of gastritis/ulcer?]. | | 1986 | 3747663 |
| pressure evaluation of the antireflux ability of the rat ureterovesical junction. | the bladder pressure necessary to cause vesicoureteral reflux (vur) was measured in 16 female rats. under general anesthesia, the ureters were exposed via an abdominal incision and a pressure catheter was placed near the ureterovesical junction. values of bladder distension and bladder pressure increase to cause vur were obtained by injecting isotonic saline in one ureter until vur in the opposite ureter was detected as a sudden pressure increase. after 5 min the same procedure was done on the c ... | 1986 | 3750580 |
| vitamin e deficiency in rabbits receiving a high pufa diet with and without a non-absorbable antioxidant. 1. incorporation of [1-14c]glycine into skeletal muscle proteins. | previous studies of metabolic changes in vitamin e-deficient animals were often difficult to interpret because the vitamin e-free, high pufa diets used in such studies may have contained high levels of peroxides. presumed effects of vitamin e deficiency could have been effects of peroxide consumption. addition of the non-absorbable polymeric antioxidant anoxomer to a semisynthetic vitamin e-free diet containing cod liver oil was found to suppress peroxide formation. nevertheless, this diet produ ... | 1986 | 3751156 |
| [reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures]. | in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ... | 1986 | 3751401 |
| [reactogenic properties and immunogenicity of measles and mumps vaccines when used by different procedures]. | in the controlled epidemiological trial the reactogenic and immunogenic properties of live measles vaccine (lmv) and live parotitis vaccine (lpv) were evaluated in identical groups of children immunized in accordance with different schedules: only lmv or lpv, the injection of these preparations mixed in one syringe or with the use of separate syringes, or at an interval of 3 months. the highest number of febrile reactions, rash and catarrhal symptoms was registered among children immunized with ... | 1986 | 3751401 |
| 1,25-dihydroxyvitamin d3 treatment results in increased choline acetyltransferase activity in specific brain nuclei. | to better understand the role of 1,25-dihydroxyvitamin d3 [1,25-(oh)2d3] in brain function, the level of calcium-binding protein (cabp) and the activities of choline acetyltransferase (cat) and monoamine oxidase were measured in discrete brain nuclei of vitamin d-deficient and -replete male rats. the nuclei sampled were those in which receptors for 1,25-(oh)2d3 and/or vitamin d-dependent cabp have been localized. significant elevations in cat activity were found in the arcuatemedian eminence and ... | 1986 | 3753932 |
| the effect of arylazido aminopropionyl atp (anapp3) on inhibition of pelvic nerve evoked contractions of the cat urinary bladder. | previously reported work has provided evidence that arylazido aminopropionyl atp (anapp3), a p2-receptor antagonist, blocks the effects of atp and other purine analogs, in the urinary bladder of the cat. this antagonism appeared limited to those effects mediated by p2-receptors, such as contraction of the urinary bladder. however, it was noted that anapp3 did alter the effects of adenosine, mediated through p1-receptors, in some preliminary experiments. a series of experiments was undertaken to ... | 1986 | 3753940 |
| direct retinal pathways to the limbic thalamus of the monkey. | tritiated proline, horseradish peroxidase (hrp), and wheat germ agglutinin conjugated to hrp (wga-hrp) were used as anterograde tracers in the monkey to reveal visual pathways. after intravitreal injections, three separate, direct routes of labeled retinal axons were followed to the thalamus. these routes eventually converged to innervate the lateral dorsal and anterodorsal thalamic nuclei. thus, retinal input may reach the posterior cingulate cortex after a single synapse in lateral and anterio ... | 1986 | 3754221 |
| [3h]pirenzepine and (-)-[3h]quinuclidinyl benzilate binding to rat cerebral cortical and cardiac muscarinic cholinergic sites. i. characterization and regulation of agonist binding to putative muscarinic subtypes. | the binding and regulation of selected muscarinic agonists to putative subtypes in rat cerebral cortex and heart were studied. parallel inhibition studies of [3h]pirenzepine ([3h]pz) and (-)-[3h]quinuclidinylbenzilate [(-)-[3h]qnb]-labeled membranes were done with and without 30 microm guanyl-5'-yl imidodiphosphate [gpp(nh)p] at 25 degrees c in 10 mm na-k-phosphate buffer which enhances pz binding affinity and in modified krebs-phosphate buffer, which mimics physiological conditions. classical a ... | 1986 | 3754580 |
| ketoconazole-induced reduction in serum 1,25-dihydroxyvitamin d. | the antimycotic agent ketoconazole is known to inhibit several cytochrome p450-dependent enzymes involved in the biosynthesis of steroid hormones from cholesterol. since 1,25-dihydroxyvitamin d is also a sterol synthesized by cytochrome p450-dependent enzymes, we assessed whether ketoconazole would lower serum 1,25-dihydroxyvitamin d levels. in nine normal men, administration of ketoconazole for 1 week in doses of 300-1200 mg/day led to a dose-dependent reduction in serum 1,25-dihydroxyvitamin d ... | 1986 | 3755445 |
| how a child's blood pressure is related to psychological influences. some results with r. zazzo's bestiary test. | the purpose of the study was to investigate the relationships between some psychological characteristics and blood pressure. we used the bestiary test which gives an impression of the child's psychological characteristics and his way of coping with personal conflicts. the test was applied to 766 children between the ages of 4-10 years. by discriminant analysis we established relations between systolic blood pressure, and the responses to the test. by correspondence analysis we found that hyperte ... | 1986 | 3757284 |
| uptake of 450191-s, a 1h-1,2,4-triazolyl benzophenone derivative, in the everted sac of rat small intestine: role of intestinal aminopeptidases. | 5-[(2-aminoacetamido)methyl]-1-[p-chloro-2-(o-chlorobenzoyl)phenyl ]-n,n-dimethyl-1 h-1,2,4-triazole-3-carboxamide hydrochloride dihydrate (450191-s), a sleep inducer, is a ring-opened derivative of 1,4-benzodiazepine and has been reported to be activated by intestinal aminopeptidases in a step of absorption. in this study, we investigated the role of the intestinal aminopeptidases in the uptake of 450191-s by the intestine by using various aminopeptidase inhibitors in everted sacs of rat small ... | 1986 | 3761138 |
| myocardial ischemia stimulates catecholamine synthesis and catabolism in the dog adrenal medulla. | the effects of regional ischemia of the myocardium upon metabolism and catabolism of catecholamines were studied in the dog adrenal medulla. regional ischemia was induced by ligating a small branch of the left anterior descending coronary artery for 1 or 24 hours. sham-operated controls were run in parallel. coronary ligation for 24 hours resulted in a decrease in the level of epinephrine and an increase in the level of dopamine in the adrenal medulla, and also resulted in an increase in the act ... | 1986 | 3761566 |
| the complex nature of food antigens: studies of cross-reacting crustacea allergens. | | 1986 | 3767090 |
| [establishment of a spontaneously transformed human fetal lung cell line and its biological characteristics]. | a spontaneously transformed human fetal lung cell line (hflt) was derived from a human fetal lung diploid fibroblast cell line (2bs) by continuous culture. the biological characteristics of this cell line were studied and compared with those of 2bs cells. accompanying the morphological alteration, the growth rate of the transformed cell was accelerated and the maximum cell density was increased. the anchorage-independent growth was shown by its ability to form colonies in soft agar. the collagen ... | 1986 | 3769747 |
| widowhood, sex, labor force participation, and the use of physician services by elderly adults. | this paper investigated the relationships of widowhood, sex, and labor force participation with the use of ambulatory physician services by elderly adults. data on 18,441 individuals aged 55 and over were taken from the 1978 health interview survey. hierarchical regression results indicated that although these three factors are related to physician utilization at the zero- and first-partial levels, only sex remained significant when their two- and three-way interactions and other variables from ... | 1986 | 3772057 |
| a human y-chromosome specific repeated dna family (dyz1) consists of a tandem array of pentanucleotides. | we have determined the complete nucleotide sequence of a 3564 bp ecori fragment which represents a major component of the human y-chromosome specific repeated dna family (dyz1). sequencing result showed a tandem array of pentanucleotides after five nucleotides were inserted or deleted at four positions. 229 out of the 713 pentanucleotides were ttcca, and 297 were its single-base substituents. southern hybridization analyses of male genomic dnas showed that several endonuclease cleavage sites wer ... | 1986 | 3774538 |
| effects of sex steroids on the development of two granule cell subpopulations in the rat accessory olfactory bulb. | the effects of postnatal male castration on day 1 (d1) after birth and female androgenization on accessory olfactory bulb (aob) light and dark granule cell populations were studied. control males showed a greater number of both light and dark aob granule cells than females. postnatal treatment reversed these differences in the light granule cells. female androgenization on d1 does not affect the number of aob dark granule cells. however, male orchidectomy also on d1 significantly decreases the n ... | 1986 | 3779445 |
| gangliosides and synaptosomal calcium homeostasis. | in this study, depolarization-dependent (net) calcium transport and binding capacity to rat brain synaptosomes was investigated under conditions affecting the ganglioside composition of the synaptoplasmatic membranes. specific interaction of the monosialoganglioside gm1 with the membrane, as observed in low (40 nm) concentration of ganglioside, resulted in significant inhibition of the maximal depolarization-induced calcium uptake. the changes in ganglioside composition of synaptosomes produced ... | 1986 | 3781769 |
| the mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype. | we describe the structure of a novel and unusually heterologous beta-tubulin isotype (m beta 1) isolated from a mouse bone marrow cdna library, and a second isotype (m beta 3) isolated from a mouse testis cdna library. comparison of m beta 1 and m beta 3 with the completed (m beta 4, m beta 5) or extended (m beta 2) sequence of three previously described beta-tubulin isotypes shows that each includes a distinctive carboxy-terminal region, in addition to multiple amino acid substitutions througho ... | 1986 | 3782288 |
| the mammalian beta-tubulin repertoire: hematopoietic expression of a novel, heterologous beta-tubulin isotype. | we describe the structure of a novel and unusually heterologous beta-tubulin isotype (m beta 1) isolated from a mouse bone marrow cdna library, and a second isotype (m beta 3) isolated from a mouse testis cdna library. comparison of m beta 1 and m beta 3 with the completed (m beta 4, m beta 5) or extended (m beta 2) sequence of three previously described beta-tubulin isotypes shows that each includes a distinctive carboxy-terminal region, in addition to multiple amino acid substitutions througho ... | 1986 | 3782288 |
| [the effect of the alkaloid narcotine and its diols on tissue histamine levels]. | | 1986 | 3786390 |
| estimation of regional gas and tissue volumes of the lung in supine man using computed tomography. | this study was intended to discover how well computed tomography could recover the volume and weight of lung like foams in a body like shell, and then how well it could recover the volume and weight of the lungs in supine man. model thoraces were made with various loaves of bread submerged in water. computed tomography scans recovered the volume of the model lungs (true volume range 250-12,500 ml) within +0.2 (sd 68) ml and their weights (true range 72-3125 g) within +30 (78) g. scans also recov ... | 1986 | 3787544 |
| methylhistidine changes in tibialis anterior muscles of 6-mercaptopurine-treated rats. | | 1986 | 3787652 |
| failure of norfloxacin to eradicate chlamydia trachomatis in nongonococcal urethritis. | norfloxacin has some activity in vitro against chlamydia trachomatis and ureaplasma urealyticum, although not at levels attainable in serum. in this study, norfloxacin was administered (400 mg orally twice daily for 10 days) to men with acute nongonococcal urethritis. of 25 men from whom c. trachomatis was initially isolated, 21 had the organism reisolated at the first follow-up visit posttreatment, and there were minimal changes in the number of inclusion-forming units in culture. ultimately, a ... | 1986 | 3789692 |
| frequency of four classes of tetracycline resistance determinants in salmonella and shigella spp. clinical isolates. | the frequencies of tetracycline resistance determinants of the classes a, b, c, and d were determined in 53 non-lactose-fermenting clinical isolates. the most frequent class of determinant in salmonella typhimurium and shigella flexneri strains was class b; however, the predominant determinant in shigella sonnei strains was class c. | 1986 | 3789700 |