| acquired haemophilia in a patient with gram-negative urosepsis and bladder cancer. | we here report a patient who developed a high titer antibody to factor viii (fviii) during gram-negative urosepsis caused by enterobacter cloacae after complete resection of rectal cancer. the patient initially presented with a life threatening spontaneous hematothorax and multiple large haematomas. coagulation studies revealed a severe fviii deficiency <1% with a high fviii antibody titer of 64 bu. the bleeding responded rapidly to infusions of recombinant factor viia. after achievement of a pa ... | 2005 | 15810923 |
| azospirillum brasilense produces the auxin-like phenylacetic acid by using the key enzyme for indole-3-acetic acid biosynthesis. | an antimicrobial compound was isolated from azospirillum brasilense culture extracts by high-performance liquid chromatography and further identified by gas chromatography-mass spectrometry as the auxin-like molecule, phenylacetic acid (paa). paa synthesis was found to be mediated by the indole-3-pyruvate decarboxylase, previously identified as a key enzyme in indole-3-acetic acid (iaa) production in a. brasilense. in minimal growth medium, paa biosynthesis by a. brasilense was only observed in ... | 2005 | 15812004 |
| quantitative detection of listeria monocytogenes in biofilms by real-time pcr. | a quantitative method based on a real-time pcr assay to enumerate listeria monocytogenes in biofilms was developed. the specificity for l. monocytogenes of primers targeting the listeriolysin gene was demonstrated using a sybr green i real-time pcr assay. the number of l. monocytogenes detected growing in biofilms was 6 x 10(2) cfu/cm2. | 2005 | 15812058 |
| identification of clinically relevant viridans streptococci by an oligonucleotide array. | viridans streptococci (vs) are common etiologic agents of subacute infective endocarditis and are capable of causing a variety of pyogenic infections. many species of vs are difficult to differentiate by phenotypic traits. an oligonucleotide array based on 16s-23s rrna gene intergenic spacer (its) sequences was developed to identify 11 clinically relevant vs. these 11 species were streptococcus anginosus, s. constellatus, s. gordonii, s. intermedius, s. mitis, s. mutans, s. oralis, s. parasangui ... | 2005 | 15814960 |
| endotoxic shock after gamete intrafallopian transfer. | to report an extremely rare case of endotoxic shock due to enterobacter cloacae that occurred after laparoscopy for gamete intrafallopian transfer (gift) in a nulligravid woman. | 2005 | 15820819 |
| hemoglobin conformation couples erythrocyte s-nitrosothiol content to o2 gradients. | it is proposed that the bond between nitric oxide (no) and the hb thiol cys-beta(93) (snohb) is favored when hemoglobin (hb) is in the relaxed (r, oxygenated) conformation, and that deoxygenation to tense (t) state destabilizes the snohb bond, allowing transfer of no from hb to form other (vasoactive) s-nitrosothiols (snos). however, it has not previously been possible to measure snohb without extensive hb preparation, altering its allostery and sno distribution. here, we have validated an assay ... | 2005 | 15824313 |
| metallo-beta-lactamases: the quiet before the storm? | the ascendancy of metallo-beta-lactamases within the clinical sector, while not ubiquitous, has nonetheless been dramatic; some reports indicate that nearly 30% of imipenem-resistant pseudomonas aeruginosa strains possess a metallo-beta-lactamase. acquisition of a metallo-beta-lactamase gene will invariably mediate broad-spectrum beta-lactam resistance in p. aeruginosa, but the level of in vitro resistance in acinetobacter spp. and enterobacteriaceae is less dependable. their clinical significan ... | 2005 | 15831827 |
| isolation of silver- and antibiotic-resistant enterobacter cloacae from teeth. | antibiotic-resistant bacteria pose a serious threat to human health; hence the mechanisms that lead to their selection need to be investigated. one possible mechanism is that the silver and mercury in amalgam dental restorations may select for bacteria that contain heavy metal and antibiotic-resistance determinants, leading to the spread of these resistances, particularly if they are contained on the same mobile genetic element. the incidence of silver-resistant bacteria on teeth is investigated ... | 2005 | 15836522 |
| genetic environment and expression of the extended-spectrum beta-lactamase blaper-1 gene in gram-negative bacteria. | the genetic location of the gene coding for the expanded-spectrum beta-lactamase per-1 was analyzed in a series of gram-negative isolates. it was identified as part of a composite transposon bracketed by two novel insertion elements, ispa12 and ispa13, belonging to the is4 family that possess transposases that share 63% amino acid identity and that are chromosomally located in pseudomonas aeruginosa, providencia stuartii, and acinetobacter baumannii. on the contrary, the bla(per-1) gene was iden ... | 2005 | 15855485 |
| evaluation by monte carlo simulation of the pharmacokinetics of two doses of meropenem administered intermittently or as a continuous infusion in healthy volunteers. | meropenem is a broad-spectrum carbapenem antibacterial agent. in order to optimize levels in plasma relative to the mics, the ideal dose level and dosage regimen need to be determined. the pharmacokinetics of meropenem were studied in two groups, each comprising eight healthy volunteers who received the following doses: 500 mg as an intravenous infusion over 30 min three times a day (t.i.d.) versus a 250-mg loading dose followed by a 1,500 mg continuous infusion over 24 h for group a and 1,000 m ... | 2005 | 15855510 |
| further identification of plasmid-mediated quinolone resistance determinant in enterobacteriaceae in turkey. | | 2005 | 15855552 |
| the iron-siderophore transporter fhua is the receptor for the antimicrobial peptide microcin j25: role of the microcin val11-pro16 beta-hairpin region in the recognition mechanism. | the role of the outer-membrane iron transporter fhua as a potential receptor for the antimicrobial peptide mccj25 (microcin j25) was studied through a series of in vivo and in vitro experiments. the requirement for both fhua and the inner-membrane tonb-exbb-exbd complex was demonstrated by antibacterial assays using complementation of an fhua(-) strain and by using isogenic strains mutated in genes encoding the protein complex respectively. in addition, mccj25 was shown to block phage t5 infecti ... | 2005 | 15862112 |
| chemical forms of selenium in the metal-resistant bacterium ralstonia metallidurans ch34 exposed to selenite and selenate. | ralstonia metallidurans ch34, a soil bacterium resistant to a variety of metals, is known to reduce selenite to intracellular granules of elemental selenium (se(0)). we have studied the kinetics of selenite (se(iv)) and selenate (se(vi)) accumulation and used x-ray absorption spectroscopy to identify the accumulated form of selenate, as well as possible chemical intermediates during the transformation of these two oxyanions. when introduced during the lag phase, the presence of selenite increase ... | 2005 | 15870319 |
| prevalence of derepressed ampc mutants and extended-spectrum beta-lactamase producers among clinical isolates of citrobacter freundii, enterobacter spp., and serratia marcescens in korea: dissemination of ctx-m-3, tem-52, and shv-12. | the resistance mechanism of extended-spectrum cephalosporins in clinical isolates of citrobacter freundii, enterobacter spp., and serratia marcescens was studied. of 152 isolates, 45 isolates (29.6%) were derepressed ampc mutants and 39 isolates (25.7%) produced extended-spectrum beta-lactamase (esbls). the most prevalent esbls were ctx-m enzymes, followed by tem-52 and shv-12. | 2005 | 15872281 |
| practical application of dynamic temperature profiles to estimate the parameters of the square root model. | optimal experimental design for parameter estimation (oed/pe) is a promising method to improve parameter estimation accuracy and minimise experimental effort in the field of predictive microbiology. in this paper, the oed/pe methodology was applied on two practical examples: the growth of bacillus cereus and enterobacter cloacae in liquid whole egg product. both strains were recovered from samples of a commercial product. the goal of the modelling exercise was to quantify the influence of temper ... | 2005 | 15878409 |
| gram-negative bacteria from the camel tick hyalomma dromedarii (ixodidae) and the chicken tick argas persicus (argasidae) and their antibiotic sensitivities. | a total of nine species of gram-negative bacteria were isolated from organs and haemolymph of the hard tick hyalomma (hyalomma) dromedarii and the soft tick argas (persicargas) persicus. four species namely serratia liquefaciens, stenotrophomonas maltophilia, klebsiella ornithinolytica and aeromonas hydrophila were isolated from h. dromedarii and five species namely rahnella aquatilis, pseudomonas fluorescens, enterobacter cloacae, chryseomonas luteola and chryseobacterium meningosepticum were i ... | 2005 | 15880998 |
| pore formation, polymerization, hemolytic and leukotoxic effects of a new enterobacter cloacae toxin neutralized by antiserum. | a new toxin of enterobacter cloacae was purified and studied by sds-page electrophoresis with the purpose of investigating its ability to generate polymers and their molecular mass. monomer of 13.3 kda and structures of multimeric mass were detected. the toxin of 66 kda was the most abundant form of toxin. this polymer and the monomer were selected to examine blood cells damage. membrane pores caused by both toxin forms seemed to be of similar dimension (estimated in 3.6 nm by experiments with o ... | 2005 | 15881838 |
| bacteremia in patients with hematological malignancies. | an appropriate regimen in the empirical therapy of neutropenic fever episodes must be individualized at each institution. hospitals have different patterns of microbial isolates and antibiotic resistance that must be taken into account. the aim of this study was to investigate isolates of bacteremia and their antibiotic susceptibility in patients with hematological malignancies. | 2005 | 15886475 |
| the d-methyl group in beta-lactamase evolution: evidence from the y221g and gc1 mutants of the class c beta-lactamase of enterobacter cloacae p99. | the beta-lactam antibiotics act through their inhibition of d-alanyl-d-alanine transpeptidases (dd-peptidases) that catalyze the last step of bacterial cell wall synthesis. bacteria resist beta-lactams by a number of mechanisms, one of the more important of which is the production of beta-lactamases, enzymes that catalyze the hydrolysis of these antibiotics. the serine beta-lactamases are evolutionary descendants of dd-peptidases and retain much of their structure, particularly at the active sit ... | 2005 | 15895997 |
| characterization of an exopolysaccharide produced by a marine enterobacter cloacae. | an exopolysaccharide producing marine bacterium, enterobacter cloacae, was isolated from marine sediment collected from gujarat coast, india. chemical investigation of exopolysaccharide (eps 71 a) revealed that this exopolysaccharide was an acidic polysaccliaride containing high amount of uronic acid, fucose and sulfate which is rare for bacterial exopolysaccharides. eps 71a was found to have fucose, galactose, glucose and glucuronic acid in a molar ratio of 2: 1: 1: 1. | 2005 | 15900914 |
| reassignment of enterobacter dissolvens to enterobacter cloacae as e. cloacae subspecies dissolvens comb. nov. and emended description of enterobacter asburiae and enterobacter kobei. | the taxonomic position of enterobacter dissolvens was re-evaluated based on the analysis of the type strain atcc 23373t and three clinical isolates. the strains were assigned to the genetic cluster of the species by phylogenetic sequence analysis in the frame of a recent population genetic study. the relatedness of e. dissolves to the other species of the e. cloacae complex was analyzed by dna-dna hybridization studies based on melting profiles in microplates. the genetic cluster of e. dissolven ... | 2005 | 15900966 |
| description of enterobacter ludwigii sp. nov., a novel enterobacter species of clinical relevance. | a new species, enterobacter ludwigii, is presented on the basis of the characteristics of 16 strains, which were isolated from clinical specimens. these bacteria form a distinct genetic cluster in phylogenetic analyses of the population structure of the enterobacter cloacae complex. as determined by dna-dna cross-hybridization experiments in microplates, this genetic cluster can be delineated from the other species of the e. cloacae complex with deltatm values equal to or above 5 degrees c with ... | 2005 | 15900967 |
| enterobacter radicincitans sp. nov., a plant growth promoting species of the family enterobacteriaceae. | a plant growth promoting bacterial isolate (d5/23t) from the phyllosphere of winter wheat, able to fix atmospheric nitrogen and to produce auxines and cytokinins was investigated in a polyphasic taxonomy approach. phylogenetic analyses using the 16s rrna gene sequence of the strain clearly indicated that the strain belonged to the family enterobacteriaceae, most closely related to enterobacter cloacae with 99.0% and enterobacter dissolvens with 98.5% sequence similarity. phylogenetic analysis de ... | 2005 | 15900968 |
| purification and characterization of allophanate hydrolase (atzf) from pseudomonas sp. strain adp. | atzf, allophanate hydrolase, is a recently discovered member of the amidase signature family that catalyzes the terminal reaction during metabolism of s-triazine ring compounds by bacteria. in the present study, the atzf gene from pseudomonas sp. strain adp was cloned and expressed as a his-tagged protein, and the protein was purified and characterized. atzf had a deduced subunit molecular mass of 66,223, based on the gene sequence, and an estimated holoenzyme molecular mass of 260,000. the acti ... | 2005 | 15901697 |
| cryptic plasmid prk2 from escherichia coli w: sequence analysis and segregational stability. | cryptic plasmid prk2 of the strain escherichia coli w (atcc 9637), an ancestor of production strains for penicillin g acylase, was sequenced and characterized. based on the data on replication region and origin (ori sequence aac, 924-926nt), the plasmid was classified as cole1-like plasmid. dna sequence analysis revealed five orfs hypothetical products of which shared a significant sequence similarity with putative proteins encoded by dna of plasmid pcole1. orf1 codes for protein rom involved in ... | 2005 | 15907542 |
| bacterial cell-to-cell signaling in the gastrointestinal tract. | | 2005 | 15908344 |
| respiratory pathogens in dental plaque of hospitalized patients with chronic lung diseases. | bacterial cultivation studies have shown that dental plaque is a reservoir for respiratory pathogens in intensive care unit patients and in elderly who are debilitated, hospitalized or in a nursing home, placing them at risk of bacterial pneumonia. no information is available, however, concerning dental plaque as a reservoir of putative respiratory pathogens in hospitalized patients with chronic lung diseases. supragingival plaque colonization of 34 hospitalized chronic lung-diseased romanian ci ... | 2005 | 15909174 |
| the rising influx of multidrug-resistant gram-negative bacilli into a tertiary care hospital. | the prevalence of multidrug resistance (mdr) among gram-negative bacilli is rapidly increasing. quantification of the prevalence and the common antimicrobial coresistance patterns of mdr gram-negative bacilli (mdr-gnb) isolates recovered from patients at hospital admission, as well as identification of patients with a high risk of harboring mdr-gnb, would have important implications for patient care. | 2005 | 15909268 |
| temporal changes in bacterial resistance in german intensive care units, 2001-2003: data from the sari (surveillance of antimicrobial use and antimicrobial resistance in intensive care units) project. | conservative testing revealed a stable antibiotic resistance situation for staphylococcus aureus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae and enterobacter cloacae in 32 german intensive care units (icus) actively participating in the sari (surveillance of antimicrobial use and antimicrobial resistance in icus) project over a three-year period (2001--2003). no significant changes were shown for methicillin-resistant s. aureus (mrsa) (p=0.501; the mrsa rate increased in 18 ... | 2005 | 15923060 |
| efficacy and safety of telithromycin 800 mg once daily for 7 days in community-acquired pneumonia: an open-label, multicenter study. | community-acquired pneumonia (cap) remains a major cause of morbidity and mortality throughout the world. telithromycin (a new ketolide) has shown good in vitro activity against the key causative pathogens of cap, including s pneumoniae resistant to penicillin and/or macrolides. | 2005 | 15927060 |
| evolutionary relationships of three new species of enterobacteriaceae living as symbionts of aphids and other insects. | ecological studies on three bacterial lineages symbiotic in aphids have shown that they impose a variety of effects on their hosts, including resistance to parasitoids and tolerance to heat stress. phylogenetic analyses of partial sequences of gyrb and reca are consistent with previous analyses limited to 16s rrna gene sequences and yield improved confidence of the evolutionary relationships of these symbionts. all three symbionts are in the enterobacteriaceae. one of the symbionts, here given t ... | 2005 | 15933033 |
| microbial contamination of hematopoietic progenitor cell grafts-incidence, clinical outcome, and cost-effectiveness: an analysis of 735 grafts. | screening of progenitor cell grafts (marrow, peripheral blood, and cord blood) for microbial contamination is required by the standards of aabb. clinical sequelae from infusion of these contaminated grafts, however, is uncommon. | 2005 | 15934984 |
| enterobacter meningitis: organism susceptibilities, antimicrobial therapy and related outcomes. | meningitis due to enterobacter species is an uncommon infection in adults; however, when present, treatment is frequently complicated by resistance of many enterobacter isolates to third-generation cephalosporins and poor central nervous system penetration of other antibiotics. the aim of this study was to retrospectively review cases of meningitis caused by enterobacter species at our institution, to better characterize patient factors, pathogen characteristics, and treatment options for this i ... | 2005 | 15936376 |
| [an investigation of bacterial ecology and analysis of bacterial resistance to antibiotics in a burn ward in nanning district]. | to investigate the changes in the bacterial ecology and to analyze the bacterial resistance to antibiotics in a burn ward in nanning district during the past 15 years, so as to provide reference to the clinical management of burn infection under subtropical climate. | 2005 | 15938956 |
| practical methods using boronic acid compounds for identification of class c beta-lactamase-producing klebsiella pneumoniae and escherichia coli. | detection of the resistance mediated by class c beta-lactamases remains a challenging issue, considering that transferable plasmid-mediated class c beta-lactamases are of worldwide concern. methods for the identification of strains that produce extended-spectrum beta-lactamases (esbls) or metallo-beta-lactamases (mbls) have been developed and applied for routine use in clinical microbiology laboratories, but no practical methods for identification of plasmid-mediated class c producers have been ... | 2005 | 15956362 |
| gene mutations responsible for overexpression of ampc beta-lactamase in some clinical isolates of enterobacter cloacae. | ampc regulatory genes in 21 ceftazidime-resistant clinical isolates of enterobacter cloacae (mics of > or = 16 microg/ml) were characterized. all isolates exhibited ampc overproduction due to ampd mutation. additionally, we found two ampr mutants among the isolates. this is the first report of chromosomal ampr mutation in clinical isolates of e. cloacae. | 2005 | 15956430 |
| first identification of clostridium celerecrescens in liquid drained from an abscess. | we present the first report on the characterization of clostridium celerecrescens isolated from liquid drained via abscess puncture from a 45-year-old man. the organism was identified by sequence comparison of 16s rrna genes. | 2005 | 15956446 |
| [characteristics of selected virulence factors of enterobacter cloacae strains isolated from clinical specimens]. | we demonstrated that enterobacter cloacae possesses a selective haemolytic activity on sheep erythrocytes. all the screened strains showed a haemolytic activity on sheep erythrocytes when cultures were preincubated with beta-mercaptoethanol. the investigation circulation of the genes encoding extended spectrum beta-lactamases (esbl) shows that beta-lactamase producers can be ascribed to specific patterns of plasmids. we also demonstrated that genetic material from e. coli can be transferred and ... | 2004 | 15959991 |
| spread of novel expanded-spectrum beta-lactamases in enterobacteriaceae in a university hospital in the paris area, france. | in 2002, 28 non-duplicate enterobacterial isolates producing extended-spectrum beta-lactamases (esbls) were collected from infected patients at the bicêtre hospital in paris, france. escherichia coli was the predominant esbl-positive enterobacterial species, comprising ten (36%) of the isolates. ctx-m enzymes (ctx-m-3, ctx-m-10, ctx-m-14 and ctx-m-15) were produced by 11 (39%) of the isolates (six e. coli, two enterobacter cloacae, one enterobacter aerogenes, one proteus mirabilis and one citrob ... | 2005 | 15966981 |
| two new sinorhizobium meliloti lysr-type transcriptional regulators required for nodulation. | the establishment of an effective nitrogen-fixing symbiosis between sinorhizobium meliloti and its legume host alfalfa (medicago sativa) depends on the timely expression of nodulation genes that are controlled by lysr-type regulators. ninety putative genes coding for lysr-type transcriptional regulators were identified in the recently sequenced s. meliloti genome. all 90 putative lysr genes were mutagenized using plasmid insertions as a first step toward determining their roles in symbiosis. two ... | 2005 | 15968067 |
| an enterobacter cloacae toxin able to generate oxidative stress and to provoke dose-dependent lysis of leukocytes. | we investigated an enterobacter cloacae strain exhibiting high hemolytic and leukotoxic activity. monomeric and polymeric forms of the toxin showed similar effects on blood cells, although the polymer was more active than the monomer. fluorescence microscopy revealed that both forms of the fitc-labeled toxin interacted with leukocytes, principally with neutrophils. prelytic concentrations of polymeric and monomeric toxin significantly increased the production of reactive oxygen species (ros) in ... | 2005 | 15969471 |
| isolation and characterization of enteric bacteria from the hindgut of formosan termite. | the formosan subterranean termite, coptotermes formosanus shiraki, is an aggressive, invasive termite species that has caused billions of dollars of damage across the united states for the past 50 years. termites depend on intestinal microorganisms for cellulose digestion. symbiotic microorganisms in the termite gut play key physiological functions such as cellulose and hemicellulose digestion, acetogenesis, hydrogenesis, methanogenesis, sulfate reduction, and nitrogen fixation. additionally, in ... | 2005 | 15978992 |
| distribution of genes encoding the microbial non-oxidative reversible hydroxyarylic acid decarboxylases/phenol carboxylases. | bacterial non-oxidative, reversible multi subunit hydroxyarylic acid decarboxylases/phenol carboxylases are encoded by the three clustered genes, b, c, and d, of approximately 0.6, 1.4, and 0.2 kb, respectively. there are more than 160 homologues in the database with significant similarity to gene b (homology to ubix) and c (ubid) distributed in all three microbial domains, however, homologues to gene d, are not numerous ( approximately 15). the occurrence of the entire bcd gene cluster encoding ... | 2005 | 15979273 |
| antimicrobial activity of novel dendrimeric peptides obtained by phage display selection and rational modification. | a large 10-mer phage peptide library was panned against whole escherichia coli cells, and an antimicrobial peptide (qekirvrlsa) was selected. the peptide was synthesized in monomeric and dendrimeric tetrabranched form (multiple antigen peptide [map]), which generally allows a dramatic increase of peptide stability to peptidases and proteases. the antibacterial activity of the dendrimeric peptide against e. coli was much higher than that of the monomeric form. modification of the original sequenc ... | 2005 | 15980334 |
| worldwide disseminated arma aminoglycoside resistance methylase gene is borne by composite transposon tn1548. | the arma (aminoglycoside resistance methylase) gene, which confers resistance to 4,6-disubstituted deoxystreptamines and fortimicin, was initially found in klebsiella pneumoniae bm4536 on incl/m plasmid pip1204 of ca. 90 kb which also encodes the extended-spectrum beta-lactamase ctx-m-3. thirty-four enterobacteria from various countries that were likely to produce a ctx-m enzyme since they were more resistant to cefotaxime than to ceftazidime were studied. the arma gene was detected in 12 clinic ... | 2005 | 15980373 |
| in vitro synergy of ciprofloxacin and gatifloxacin against ciprofloxacin-resistant pseudomonas aeruginosa. | multidrug-resistant pseudomonas aeruginosa with combined decreased susceptibility to ceftazidime, ciprofloxacin, imipenem, and piperacillin is increasingly being found as a cause of nosocomial infections. it is important to look for combinations of drugs that might be synergistic. ciprofloxacin resistance by p. aeruginosa is mediated in part by an efflux pump mechanism. gatifloxacin, an 8-methoxyfluoroquinolone, inhibits a staphylococcal efflux pump. an earlier in vitro study using an etest syne ... | 2005 | 15980375 |
| broader distribution of plasmid-mediated quinolone resistance in the united states. | the plasmid-encoded quinolone resistance gene qnra confers low-level quinolone resistance, facilitating selection of higher-level resistance. epidemiologic surveys for qnra were extended to isolates of enterobacter spp. and to quinolone-susceptible enterobacteriaceae. two (10%) of 20 ceftazidime-resistant quinolone-susceptible klebsiella pneumoniae strains carried the gene, as did 12 (17%) of 71 ceftazidime-resistant enterobacter strains from across the united states. one of these enterobacter i ... | 2005 | 15980384 |
| association of plasmid-mediated quinolone resistance with extended-spectrum beta-lactamase veb-1. | association of the plasmid-mediated quinolone resistance determinant qnra and the bla(veb-1) gene was identified in a single enterobacter cloacae isolate from k.-bicêtre, france, and in 11 out of 23 bla(veb-1)-positive enterobacterial isolates from bangkok, thailand. this result may explain in part the association between quinolone and extended-spectrum beta-lactam resistance. | 2005 | 15980408 |
| gram-negative bacilli associated with catheter-associated and non-catheter-associated bloodstream infections and hand carriage by healthcare workers in neonatal intensive care units. | bloodstream infections caused by gram-negative bacilli are a substantial cause of morbidity and mortality in infants in neonatal intensive care units. this study describes the species of gram-negative bacilli causing bloodstream infections in two neonatal intensive care units, compares characteristics of catheter-related and non-catheter-related bloodstream infections, and compares species and antibiotic resistance patterns of these organisms with those isolated from the hands of nurses working ... | 2005 | 15982435 |
| in-house validation of the bactec 9240 blood culture system for detection of bacterial contamination in platelet concentrates. | at present, only two commercially available automated culture systems are cleared by the fda for the purpose of quality control (qc) testing for bacterial contamination of platelet (plt) concentrates: the bact/alert blood culture system (biomérieux) and the pall ebds (pall corporation), both of which allow testing of leukoreduced apheresis as well as whole blood-derived plts. after the decision of the aabb to institute universal qc testing of plt concentrates for evidence of bacterial contaminat ... | 2005 | 15987359 |
| adhesion of the probiotic bacterium lactobacillus plantarum 299v onto the gut mucosa in critically ill patients: a randomised open trial. | to achieve any possible positive effect on the intestinal mucosa cells it is important that probiotics adhere tightly onto the intestinal mucosa. it has been shown in healthy volunteers that lactobacillus plantarum 299v (lp 299v) (dsm 9843), a probiotic bacterium, given orally in a fermented oatmeal formula adheres onto the intestinal mucosa, but whether this also occurs in critically ill patients is unknown. | 2005 | 15987403 |
| accelerating bacterial identification by infrared spectroscopy by employing microarray deposition of microorganisms. | a microarray method for the deposition of bacteria onto an agar slide was developed to accelerate the formation of microcolonies. representative microarrays each consisting of 40 micro-spots of five replicates of eight foodborne bacteria (yersinia enterocolitica, staphylococcus aureus, salmonella typhimurium, listeria monocytogenes, enterobacter cloacae, citrobacter freundii, klebsiella pneumoniae, and escherichia coli) were printed on a brain heart infusion (bhi) agar slide using a contact micr ... | 2004 | 15992277 |
| prevalence of decreased susceptibility to carbapenems among serratia marcescens, enterobacter cloacae, and citrobacter freundii and investigation of carbapenemases. | between march and july 2002, total of 612 clinical isolates of serratia marcescens, enterobacter cloacae, and citrobacter freundii (201 s. marcescens, 228 e. cloacae, and 183 c. freundii) were collected from 13 clinical laboratories in a nationwide distribution. imipenem and meropenem minimum inhibitory concentrations (mics) were determined using the agar dilution method according to the national committee for clinical laboratory standards guidelines. for the isolates with a decreased susceptibi ... | 2005 | 15994052 |
| [antimicrobial activity of fosfomycin under various conditions against standard strains, beta-lactam resistant strains, and multidrug efflux system mutants]. | the purpose of this study was to evaluate the possible benefit of fosfomycin (fom) as prophylactic antibiotic in terms of antimicrobial activity and the potential of inducibility of beta-lactamase, compared with cefazolin, cefotiam, cefmetazole, and piperacillin that are commonly used as perioperative agents. the in vitro activity of fom against aerobic gram-negative bacteria using mueller-hinton agar or nutrient agar supplemented with glucose-6-phosphate (g6p) as tested medium increased within ... | 2005 | 15997654 |
| [bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between april 2003 and march 2004]. | tendency of isolated bacteria from infections in general surgery during the period from april 2003 to march 2004 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 455 strains including 14 strains of candida spp. were isolated from 191(75.2%) of 254 patients with surgical infections. two hundred and thirty-nine strains were isolated from primary infections, and 216 strains were isolated from postoperative infections. from primary infection ... | 2005 | 15997655 |
| ampc disk test for detection of plasmid-mediated ampc beta-lactamases in enterobacteriaceae lacking chromosomal ampc beta-lactamases. | although plasmid-mediated ampc beta-lactamases were first reported in the late 1980s, many infectious disease personnel remain unaware of their clinical importance. these enzymes are typically produced by isolates of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. and are associated with multiple antibiotic resistance that leaves few therapeutic options. plasmid-mediated ampc beta-lactamases have been associated with false in vitro susceptibility to cephalosporins. many ... | 2005 | 16000421 |
| detection of pseudomonas aeruginosa producing metallo-beta-lactamases in a large centralized laboratory. | metallo-beta-lactamases (mbls) have been increasingly recognized from clinical isolates worldwide, but the laboratory detection of these strains is not well defined. we report a study that developed an edta disk screen test and a molecular diagnostic assay for the detection of mbl-producing pseudomonas aeruginosa. using nccls disk methodology, inhibition zone diameters were determined in tests with imipenem (ipm) and meropenem (mem) disks alone and in combination with 930 microg of edta. this te ... | 2005 | 16000424 |
| frequent carriage of panton-valentine leucocidin genes by staphylococcus aureus isolates from surgically drained abscesses. | between 1 february and 15 april 2002, 95 patients were admitted to gaston bourret territorial hospital (new caledonia, france) for drainage of community-acquired soft tissue abscesses. staphylococcus aureus was detected in 68 cases (72%). two-thirds of the patients with s. aureus infection had furuncles, which were located on the limbs in 82% of cases. the median interval between symptom onset and hospital admission was 5.7 days. three-quarters of the patients were melanesians living in tribes. ... | 2005 | 16000436 |
| comparative recovery of microorganisms from bact/alert plastic and glass fa and fn blood culture bottles. | biomerieux, inc., has recently introduced plastic bottles to replace glass bottles for use in the bact/alert blood culture system. we compared the performance of the plastic to the glass bottles in a large clinical evaluation. two blood cultures were collected from each patient, one using glass fa (aerobic) and fn (anaerobic) bottles and one using plastic fa and fn bottles. of the 4,040 sets of four bottles collected, 3,110 contained the recommended 8 to 12 ml of blood, yielding 524 microorganis ... | 2005 | 16000442 |
| development and evaluation of chlamylege, a new commercial test allowing simultaneous detection and identification of legionella, chlamydophila pneumoniae, and mycoplasma pneumoniae in clinical respiratory specimens by multiplex pcr. | this study describes the development and evaluation of a new commercial test, chlamylege (argene inc.), which allows the simultaneous detection in respiratory samples of chlamydophila pneumoniae, mycoplasma pneumoniae, and most legionella species, as well as pcr inhibitors, by using a multiplex pcr and microplate hybridization. the sensitivities of chlamylege were 1 x 10(-3) ifu, 5 x 10(-2) color-changing units, and 1 cfu per reaction tube for c. pneumoniae, m. pneumoniae, and legionella pneumop ... | 2005 | 16000443 |
| extended-spectrum-beta-lactamase-producing enterobacteriaceae in yaounde, cameroon. | organisms producing extended-spectrum beta-lactamases (esbls) have been reported in many countries, but there is no information on the prevalence of esbl-producing members of the family enterobacteriaceae in cameroon. a total of 259 enterobacteriaceae strains were isolated between 1995 and 1998 from patients at the yaounde central hospital in cameroon. enterobacterial isolates resistant to extended-spectrum cephalosporin and monobactam were screened for esbl production by the double-disk (dd) sy ... | 2005 | 16000447 |
| enterobacter hormaechei subsp. oharae subsp. nov., e. hormaechei subsp. hormaechei comb. nov., and e. hormaechei subsp. steigerwaltii subsp. nov., three new subspecies of clinical importance. | six species and six additional genovars are combined within the so-called enterobacter cloacae complex, with one of them being the species enterobacter hormaechei. in a recent population genetic study, two genetic clusters were found in close phylogenetic proximity to the genetic cluster of e. hormaechei. in order to prove the hypothesis that these three genetic clusters belong to the same species, we performed cross-hybridization experiments in microplates with dnas of representatives of each g ... | 2005 | 16000451 |
| countrywide spread of community- and hospital-acquired extended-spectrum beta-lactamase (ctx-m-15)-producing enterobacteriaceae in lebanon. | a prospective study was carried out to assess the extent of carriage of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae at both hospital and community levels in lebanon. a total of 1,442 fecal samples were collected from hospital-based patients and 58 from health care workers of six lebanese tertiary care general hospitals located in different areas of lebanon between january and march 2003. a total of 382 fecal samples were also collected from healthy subjects between april ... | 2005 | 16000453 |
| quantitative detection of clostridium perfringens in the broiler fowl gastrointestinal tract by real-time pcr. | strains of clostridium perfringens are a frequent cause of food-borne disease and gas gangrene and are also associated with necrotic enteritis in chickens. to detect and quantify the levels of c. perfringens in the chicken gastrointestinal tract, a quantitative real-time pcr assay utilizing a fluorogenic, hydrolysis-type probe was developed and utilized to assay material retrieved from the broiler chicken cecum and ileum. primers and probe were selected following an alignment of 16s rdna sequenc ... | 2005 | 16000804 |
| rapid method for enumeration of viable legionella pneumophila and other legionella spp. in water. | a sensitive and specific method has been developed to enumerate viable l. pneumophila and other legionella spp. in water by epifluorescence microscopy in a short period of time (a few hours). this method allows the quantification of l. pneumophila or other legionella spp. as well as the discrimination between viable and nonviable legionella. it simultaneously combines the specific detection of legionella cells using antibodies and a bacterial viability marker (chemchrome v6), the enumeration bei ... | 2005 | 16000824 |
| case of emphysematous pyelonephritis in a renal allograft. | emphysematous pyelonephritis is a rare, but serious complication after renal transplantation. this is a case report of a 49-yr-old female who had a renal transplant for diabetic nephropathy. she presented to er with about 1 wk history of right lower abdominal pain and fever 15 months after the transplant. she had gross hematuria and mental status changes just prior to presentation. a computed tomography (ct) scan of the abdomen showed extensive retroperitoneal and extraperitoneal air dissecting ... | 2005 | 16008605 |
| selectivity and specificity of a chromogenic medium for detecting vibrio parahaemolyticust. | the thiosulfate-citrate-bile salts-sucrose agar (tcbs) used in the most-probable-number method for detecting vibrio parahaemolyticus cannot differentiate growth of v. parahaemolyticus from vibrio vulnificus or vibrio mimicus. this study examined the selectivity and specificity of bio-chrome vibrio medium (bcvm), a chromogenic medium that detects v. parahaemolyticus on the basis of the formation of distinct purple colonies on the medium. a panel consisting of 221 strains of bacteria, including 17 ... | 2005 | 16013386 |
| [enterobacter cloacae spondylodiscitis through misuse of high-dose intravenous buprenorphine]. | we report a case of enterobacter cloacae spondylodiscitis related to risk practices in intravenous drug addicts (ivda). | 2005 | 16026126 |
| acquisition of resistant bowel flora during a double-blind randomized clinical trial of ertapenem versus piperacillin-tazobactam therapy for intraabdominal infections. | bowel colonization with resistant bacteria can develop in patients receiving broad-spectrum antimicrobial therapy. we compared the impact of two antimicrobial regimens often used to treat intraabdominal infections on susceptibility patterns of bowel flora at the end of therapy. in a double-blind clinical trial, adults with complicated intraabdominal infection requiring surgery were randomized to receive piperacillin-tazobactam (3.375 g every 6 h) or ertapenem (1 g once a day) for 4 to 14 days. r ... | 2005 | 16048928 |
| in vitro and in vivo antibacterial activities of cs-023 (ro4908463), a novel parenteral carbapenem. | cs-023 (ro4908463, formerly r-115685) is a novel 1beta-methylcarbapenem with 5-substituted pyrrolidin-3-ylthio groups, including an amidine moiety at the c-2 position. its antibacterial activity was tested against 1,214 clinical isolates of 32 species and was compared with those of imipenem, meropenem, ceftazidime, ceftriaxone, ampicillin, amikacin, and levofloxacin. cs-023 exhibited a broad spectrum of activity against gram-positive and -negative aerobes and anaerobes, including methicillin-res ... | 2005 | 16048932 |
| origin of plasmid-mediated quinolone resistance determinant qnra. | plasmid-mediated resistance to quinolones is increasingly reported in studies of enterobacteriaceae. using a pcr-based strategy, a series of gram-negative species were screened for qnra-like genes. shewanella algae, an environmental species from marine and fresh water, was identified as its reservoir. this is a one of the very few examples of progenitor identification of an acquired antibiotic resistance gene. | 2005 | 16048974 |
| in vivo transfer of plasmid-encoded acc-1 ampc from klebsiella pneumoniae to escherichia coli in an infant and selection of impermeability to imipenem in k. pneumoniae. | we describe in vivo selection of a klebsiella pneumoniae strain with diminished imipenem susceptibility attributable to plasmid-encoded acc-1 beta-lactamase production and loss of a 36-kda major outer membrane protein, together with transfer of this plasmid from k. pneumoniae to escherichia coli in a tunisian infant. | 2005 | 16048985 |
| use of the roche lightcycler strep b assay for detection of group b streptococcus from vaginal and rectal swabs. | the results for a real-time pcr assay, using the lightcycler strep b analyte-specific reagents (roche diagnostics corporation, indianapolis, ind.), were compared to a direct plate method combined with a broth enrichment culture method for detection of group b streptococcus colonization in pregnant women. two separate evaluations were conducted using two different automated nucleic extraction instruments, the magna pure lc instrument (roche diagnostics corporation) and the lower-capacity magna pu ... | 2005 | 16081949 |
| enteric fever-like illness caused by infection with citrobacter amalonaticus. | 'enteric fever' is a potentially fatal, severe systemic disease, which is encountered worldwide. traditionally, enteric fever refers to a bacteremic illness caused by members of certain salmonella serotypes, notably: salmonella typhi, a gram-negative bacterium, and to a lesser extent, salmonella paratyphi a, b and c. in addition, other non-salmonella organisms may produce a syndrome clinically indistinguishable from "enteric fever". brucella sp., campylobacter sp., edwardsiella tarda, enterobact ... | 2005 | 16083226 |
| allophanate hydrolase, not urease, functions in bacterial cyanuric acid metabolism. | growth substrates containing an s-triazine ring are typically metabolized by bacteria to liberate 3 mol of ammonia via the intermediate cyanuric acid. over a 25-year period, a number of original research papers and reviews have stated that cyanuric acid is metabolized in two steps to the 2-nitrogen intermediate urea. in the present study, allophanate, not urea, was shown to be the 2-nitrogen intermediate in cyanuric acid metabolism in all the bacteria examined. six different experimental results ... | 2005 | 16085834 |
| development of pcr assays targeting genes in o-antigen gene clusters for detection and identification of escherichia coli o45 and o55 serogroups. | the escherichia coli o45 o-antigen gene cluster of strain o45:h2 96-3285 was sequenced, and conventional (singleplex), multiplex, and real-time pcr assays were designed to amplify regions in the wzx (o-antigen flippase) and wzy (o-antigen polymerase) genes. in addition, pcr assays targeting the e. coli o55 wzx and wzy genes were designed based on previously published sequences. pcr assays targeting e. coli o45 showed 100% specificity for this serogroup, whereas by pcr assays specific for e. coli ... | 2005 | 16085897 |
| fluorescein-labeled beta-lactamase mutant for high-throughput screening of bacterial beta-lactamases against beta-lactam antibiotics. | the increasing emergence of new bacterial beta-lactamases that can efficiently hydrolyze beta-lactam antibiotics to clinically inactive carboxylic acids has created an intractable problem in the treatment of bacterial infections, and it is highly desirable to develop a useful tool that can rapidly screen bacteria for beta-lactamases against a variety of antibiotic candidates in a high-throughput manner. this paper describes the use of a fluorescein-labeled beta-lactamase mutant (e166cf) as a con ... | 2005 | 16097768 |
| multidrug-resistant acinetobacter extremity infections in soldiers. | war wound infection and osteomyelitis caused by multidrug-resistant (mdr) acinetobacter species have been prevalent during the 2003-2005 military operations in iraq. twenty-three soldiers wounded in iraq and subsequently admitted to our facility from march 2003 to may 2004 had wound cultures positive for acinetobacter calcoaceticus-baumannii complex. eighteen had osteomyelitis, 2 burn infection, and 3 deep wound infection. primary therapy for these infections was directed antimicrobial agents fo ... | 2005 | 16102310 |
| isolation of mannose-binding c-type lectin from heliothis virescens pupae. | a mannose-binding c-type lectin (mbl) was isolated by affinity chromatography from heliothis virescens immune pupal hemolymph. the immune pupal hemolymph was obtained after bacterial injection of live enterobacter cloacae bacteria. mbl in mammals acts as an opsonin for phagocytosis and activates the lectin complement pathway of the innate immune response, which leads to killing of gram-negative bacteria and enveloped viruses. the affinity-purified and reduced pupal mbl showed a single band of 36 ... | 2005 | 16111657 |
| evaluation of bact/alert plastic culture bottles for use in testing pooled whole blood-derived leukoreduced platelet-rich plasma platelets with a single contaminated unit. | in certain countries, whole blood-derived platelet (plt)-rich plasma plts can only be pooled within 4 hours of transfusion. one prerequisite for prestorage pooling is the ability to detect low levels of bacteria from a single unit (approx. 10 colony-forming units [cfus]/ml) once pooled (10/6 approx. 2 cfus/ml). this study evaluated the bact/alert (biomérieux) for detection of bacteria in 1 unit of a 6-unit pool. | 2005 | 16131385 |
| antibacterial activity of zuccagnia punctata cav. ethanolic extracts. | the present study was conducted to investigate antibacterial activity of zuccagnia punctata ethanolic extract against 47 strains of antibiotic-resistant gram-negative bacteria and to identify bioactive compounds. inhibition of bacterial growth was investigated using agar diffusion, agar macrodilution, broth microdilution and bioautographic methods. zuccagnia punctata extract was active against all assayed bacteria (escherichia coli, klebsiella pneumoniae, proteus mirabilis, enterobacter cloacae, ... | 2005 | 16137849 |
| in vitro activities of 11 fluoroquinolones against 816 non-typhoidal strains of salmonella enterica isolated from finnish patients with special reference to reduced ciprofloxacin susceptibility. | the number of salmonella strains with reduced susceptibility to fluoroquinolones has increased during recent years in many countries, threatening the value of this antimicrobial group in the treatment of severe salmonella infections. | 2005 | 16143044 |
| development of a multiplex pcr and shv melting-curve mutation detection system for detection of some shv and ctx-m beta-lactamases of escherichia coli, klebsiella pneumoniae, and enterobacter cloacae in taiwan. | infection by extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae has been increasing in taiwan. accurate identification of the esbl genes is necessary for surveillance and for epidemiological studies of the mode of transmission in the hospital setting. we describe herein the development of a novel system, which consists of a multiplex pcr to identify bla(shv), bla(ctx-m-3)-like, and bla(ctx-m-14)-like genes and a modified shv melting-curve mutation detection method to rapidly di ... | 2005 | 16145096 |
| widespread transfer of resistance genes between bacterial species in an intensive care unit: implications for hospital epidemiology. | a transferable plasmid encoding shv-12 extended-spectrum beta-lactamase, tem-116, and aminoglycoside resistance was responsible for two sequential clonal outbreaks of enterobacter cloacae and acinetobacter baumannii bacteria. a similar plasmid was present among isolates of four different bacterial species. recognition of plasmid transfer is crucial for control of outbreaks of multidrug-resistant nosocomial pathogens. | 2005 | 16145160 |
| molecular analysis of colonized bacteria in a human newborn infant gut. | the complex ecosystem of intestinal microflora is estimated to harbor approximately 400 different microbial species, mostly bacteria. however, studies on bacterial colonization have mostly been based on culturing methods, which only detect a small fraction of the whole microbiotic ecosystem of the gut. to clarify the initial acquisition and subsequent colonization of bacteria in an infant within the few days after birth, phylogenetic analysis was performed using 16s rdna sequences from the dna i ... | 2005 | 16145549 |
| [study on treatment strategy for ventriculitis associated with ventriculoperitoneal shunt for hydrocephalus]. | to study the treatment strategy for ventriculitis associated with ventriculo peritoneal shunt for hydrocephalus. | 2005 | 16146606 |
| isolation of a hop-sensitive variant of lactobacillus lindneri and identification of genetic markers for beer spoilage ability of lactic acid bacteria. | we have isolated a hop-sensitive variant of the beer spoilage bacterium lactobacillus lindneri dsm 20692. the variant lost a plasmid carrying two contiguous open reading frames (orf s) designated horb(l) and horc(l) that encode a putative regulator and multidrug transporter presumably belonging to the resistance-nodulation-cell division superfamily. the loss of hop resistance ability occurred with the loss of resistance to other drugs, including ethidium bromide, novobiocin, and cetyltrimethylam ... | 2005 | 16151091 |
| longitudinal study of microbial diversity and seasonality in the mexico city metropolitan area water supply system. | in the mexico city metropolitan area (mcma), 70% of the water for 18 million inhabitants is derived from the basin of mexico regional aquifer. to provide an overview of the quality of the groundwater, a longitudinal study was conducted, in which 30 sites were randomly selected from 1,575 registered extraction wells. samples were taken before and after chlorine disinfection during both the rainy and dry seasons (2000-2001). microbiological parameters (total coliforms, fecal coliforms, streptococc ... | 2005 | 16151096 |
| a toxaphene-degrading bacterium related to enterobacter cloacae, strain d1 isolated from aged contaminated soil in nicaragua. | enterobacter sp. strain d1 is a facultative anaerobic, gram-negative heterotrophic bacterium isolated from toxaphene-contaminated soil. this organism was identified and characterized through phylogenetic and taxonomic studies. based on 16s rdna analysis, the strain d1 was clustered closely with the species enterobacter cloacae subsp. dissolvens (lmg 2683) and e. cloacae (atcc 13047t). strain d1 resembled these e. cloacae strains with respect to various biochemical and nutritional characteristics ... | 2005 | 16156121 |
| transgenic alteration of toll immune pathway in the female mosquito aedes aegypti. | reverse genetics is a powerful tool for understanding gene functions and their interactions in the mosquito innate immunity. we took the transgenic approach, in combination with the rna interference (rnai) technique, to elucidate the role of mosquito rel1, a homolog of drosophila dorsal, in regulation of toll immune pathway in the mosquito aedes aegypti. by transforming the mosquitoes with deltarel1-a or a double-stranded rna construct of rel1 driven by the female fat body-specific vitellogenin ... | 2005 | 16157887 |
| characterization of enterobacteria using maldi-tof mass spectrometry. | a method is proposed for the rapid classification of gram-negative enterobacteria using on-slide solubilization and trypsin digestion of proteins, followed by maldi-tof ms analysis. peptides were identified from tryptic digests using microsequencing by tandem mass spectrometry and database searches. proteins from the outer membrane family (omp) were consistently identified in the enterobacteria escherichia coli, enterobacter cloacae, erwinia herbicola, and salmonella typhimurium. database search ... | 2005 | 16159146 |
| nosocomial urosepsis caused by enterobacter kobei with aberrant phenotype. | enterobacter kobei is the species of the enterobacter cloacae complex, which is phenotypically most closely related to the species e. cloacae. this is the first report of infection caused by a new biotype of e. kobei. a patient with a history of urinary bladder operation developed a urosepsis with an enterobacter isolate displaying the e. cloacae phenotype. the isolate was classified to the species e. kobei by sequence analysis of the 16s-rdna, 4 protein-coding genes and enterobacterial repetiti ... | 2005 | 16168611 |
| eustachian valve endocarditis: a rare localization of right side endocarditis. a case report and review of the literature. | right-sided endocarditis occurs predominantly in intravenous drug users, in patients with pacemaker or central venous lines and in patients with congenital heart disease. the vast majority of cases involve the tricuspid valve. eustachian valve endocarditis is an uncommon disease with similar signs and symptoms of the tricuspid valve endocarditis. a series of only 16 cases of eustachian valve endocarditis are reported in the literature. | 2005 | 16171516 |
| specificity of enterobacterial repetitive intergenic consensus and repetitive extragenic palindromic polymerase chain reaction for the detection of clonality within the enterobacter cloacae complex. | an increasing number of clonal outbreaks caused by members of the e. cloacae complex is being reported. for the detection of clonality, pulsed-field gel electrophoresis (pfge) is considered the golden standard, but pcr-based methods are cheaper, easier to perform, and provide faster results. one hundred ninety-five isolates of the e. cloacae complex isolated at the university hospital grosshadern, munich, germany, were assigned to their respective genetic cluster by partial hsp60 sequencing. all ... | 2005 | 16182074 |
| in vitro and in vivo antibacterial activities of sm-216601, a new broad-spectrum parenteral carbapenem. | sm-216601 is a novel parenteral 1beta-methylcarbapenem. in agar dilution susceptibility testing, the mic of sm-216601 for 90% of the methicillin-resistant staphylococcus aureus (mrsa) strains tested (mic(90)) was 2 microg/ml, which was comparable to those of vancomycin and linezolid. sm-216601 was also very potent against enterococcus faecium, including vancomycin-resistant strains (mic(90) = 8 microg/ml). sm-216601 exhibited potent activity against penicillin-resistant streptococcus pneumoniae, ... | 2005 | 16189097 |
| genetic and enzymatic properties of metallo-beta-lactamase vim-5 from a clinical isolate of enterobacter cloacae. | a vim-5-producing enterobacter cloacae isolate (edv/1) was identified in a collection of clinical strains stored before 2002. the gene, bla(vim-5), was located on a 2,712-bp bamhi-hindiii fragment of a 23-kbp (approximately) nonconjugative plasmid (pedv5) in a class 1 integron as a single gene cassette. | 2005 | 16189133 |
| [pathogenic characters of infected bacteria after liver transplantation]. | to analyze the main pathogens of infection after the liver transplantation and their antibiotic resistant patterns. | 2005 | 16190391 |
| increasing ciprofloxacin resistance among prevalent urinary tract bacterial isolates in gaza strip, palestine. | this article presents the incidence of ciprofloxacin resistance among 480 clinical isolates obtained from patients with urinary tract infection (uti) during january to june 2004 in gaza strip, palestine. the resistance rates observed were 15.0% to ciprofloxacin, 82.5% to amoxycillin, 64.4% to cotrimoxazole, 63.1% to doxycycline, 32.5% to cephalexin, 31.9% to nalidixic acid, and 10.0% to amikacin. high resistance to ciprofloxacin was detected among acinetobacter haemolyticus (28.6%), staphylococc ... | 2005 | 16192681 |
| glucose-1-phosphatase (agpe) from enterobacter cloacae displays enhanced phytase activity. | using a screening procedure developed for detection of phytate hydrolysing enzymes, the gene agpe encoding glucose-1-phosphatase was cloned from an enterobacter cloacae vkpm b2254 plasmid library. sequence analysis revealed 78% identity on nucleotide and 79% identity on peptide level to escherichia coli glucose-1-phosphatase characterising the respective gene product as a representative of acid histidine phosphatases harbouring the rh(g/n)rxrp motif. the purified recombinant protein displayed ma ... | 2006 | 16193276 |
| assembly of cyclic enterobacterial common antigen in escherichia coli k-12. | we describe here the purification and quantification of a water-soluble cyclic form of enterobacterial common antigen (eca(cyc)) from escherichia coli k-12 as well as information regarding its subcellular location and the genetic loci involved in its assembly. structural characterization of purified eca(cyc) molecules obtained from e. coli k-12 revealed that they uniformly contained four trisaccharide repeat units, and they were substituted with from zero to four o-acetyl groups. cells from over ... | 2005 | 16199561 |