| aspergillomas of the sphenoid sinus: a series of 10 cases treated by endoscopic sinus surgery. | we report our experience with 10 cases of sphenoidal aspergillomas treated by endoscopic sinus surgery (ess). chronic symptoms such as cough, post-nasal discharge, dysphonia and even facial pain can be encountered in the history. computerised tomography and, occasionally, magnetic resonance imaging are of great help in the assessment of this disease, especially when extensive skull base involvement is present. the radiological presentation can vary from an heterogeneous to homogeneous opacity wi ... | 1996 | 8938890 |
| hypersensitivity pneumonitis in workers exposed to esparto grass (stipa tenacissima) fibers. | esparto grass (stipa tenacissima), which is commonly found in the mediterranean countries, has a wide variety of uses. five stucco makers who had cough, dyspnea, malaise, and fever after exposure to esparto fiber used in their jobs showed a significant decrease in symptoms when they were away from work. precipitating antibodies against an esparto extract were found in the sera of all patients. specific igg antibodies against the esparto extract were also demonstrated in all patient sera, as were ... | 1996 | 8939163 |
| immunoperoxidase staining for identification of aspergillus species in routinely processed tissue sections. | to evaluate the performance of an immunoperoxidase stain using the monoclonal antibody eb-a1 to detect aspergillus species in formalin fixed, paraffin wax embedded tissue. | 1996 | 8943743 |
| high-resolution computed tomography and pathologic findings in pulmonary aspergillosis: a pictorial essay. | aspergillus can cause a wide spectrum of pulmonary complications. it may colonize pre-existing cavities to form mycetomas. in asthmatic patients it may lead to a hypersensitivity reaction involving eosinophilic infiltration of the bronchial wall and mucoid impaction (a condition called allergic bronchopulmonary aspergillosis) or granulomatous inflammation (bronchocentric granulomatosis). in the immunocompromised host, aspergillus may invade blood vessels, causing hemorrhagic infarction (angioinv ... | 1996 | 8943916 |
| binding of extracellular matrix proteins to aspergillus fumigatus conidia. | as detected by confocal immunofluorescence microscopy, binding of fibronectin and laminin appeared to be associated with the protrusions present on the outer cell wall layer of resting aspergillus fumigatus conidia. flow cytometry confirmed that binding of laminin to conidia was dose dependent and saturable. laminin binding was virtually eliminated in trypsin-treated organisms, thus suggesting the protein nature of the binding site. conidia were also able to specifically adhere to laminin immobi ... | 1996 | 8945572 |
| [role of bronchoalveolar lavage and transbronchial biopsy in the diagnosis of pneumonia in patients with organ transplantation]. | pneumonia in patients with organ transplantation constitutes a very frequent cause of mortality, as a result precocious aetiologic diagnosis is indispensable. the bronchoscopic techniques, bronchoalveolar lavage (bal) and transbronchial biopsy (tbb), constitute fundamental procedures for these diagnoses. we begin this study with the aim of evaluating the profitability obtained with these procedures. 36 bronchoscopies were performed on 29 patients with organ transplantation, in all of them we rea ... | 1996 | 8948813 |
| aspergillus meningitis in an immunocompetent adult successfully treated with itraconazole. | | 1996 | 8953083 |
| muscle abscess due to aspergillus fumigatus in a patient with aids. | | 1996 | 8953087 |
| aspergillar fungemia in an infant with hepatoblastoma. | | 1996 | 8953089 |
| the chsb gene of aspergillus nidulans is necessary for normal hyphal growth and development. | the chsb gene from aspergillus nidulans encodes a class iii chitin synthase, an enzyme class found in filamentous fungi but not in yeast-like organisms. using a novel method, we isolated haploid segregants carrying a disrupted chsb allele from heterozygous diploid disruptants. the haploid disruptants grow as minute colonies that do not conidiate. hyphae from the disruptants have enlarged tips, a high degree of branching, and disorganized lateral walls. the mycelium is not deficient in chitin con ... | 1996 | 8953267 |
| biochemical characterisation of ketoconazole inhibitory action on aspergillus fumigatus. | the effect of ketoconazole on growth, sterol composition, in vitro sterol biosynthesis and p450-co complex formation and its interaction with microsomal p450 was determined. on solid medium and in liquid medium ketoconazole inhibited aspergillus fumigatus growth completely at 5 x 10(-5) m and 50% of the growth at 1.3 x 10(-5) m and 2.1 x 10(-5) m respectively. a close relationship between accumulation of 14 alpha-methyl sterols (eburicol, obtusifoliol and 14 alpha-methyl fecosterol) and depletio ... | 1996 | 8954348 |
| invasive aspergillosis of the orbit. | | 1996 | 8956603 |
| skin test reactivity to recombinant aspergillus fumigatus allergen i/a in patients with cystic fibrosis. | we evaluated the diagnostic value of recombinant aspergillus fumigatus allergen i/a (rasp f i/a) for skin testing in cystic fibrosis (cf) patients with allergic bronchopulmonary aspergillosis (abpa), allergy to a. fumigatus and in cf controls not allergic to a. fumigatus. skin prick test reactions to rasp f i/a were 8.5-fold stronger in a.-fumigatus-allergic and 3.3-fold stronger in abpa patients compared to the cf controls. three out of 6 well-characterized abpa patients had positive skin prick ... | 1996 | 8957115 |
| dexamethasone and cyclosporin a modulation of cytokine expression and specific antibody synthesis in an allergic bronchopulmonary aspergillosis murine model. | we previously demonstrated that, in c57b1/6 mice, cyclosporin a enhanced and dexamethasone inhibited the aspergillus fumigatus-induced pulmonary eosinophilia and total ige levels. to evaluate whether these effects were related to the modulation of t-lymphocyte recruitment and activation and cytokine expression, we performed immunohistochemical staining for t-cell surface marker cd3 and cd4, cell activation marker cd25, and cytokines granulocyte-macrophage colony-stimulating factor (gm-csf), inte ... | 1996 | 8957199 |
| [a study of otomycoses in yaoundé]. | the authors report the results of a six-month evaluation of otomycoses in the ent department of chu and the central hospital of yaoundé. we examined 2 592 patients of both sexes presenting with ear affections. the prevalence of otomycoses is 6,09%. this is an adult problem with both sexes equally affected. clinically the main symptoms were burning sensation in the ears, pruritus, sensation of fullness in the ear, hypoacousia and otorrhea. clinical lesions are of many types including myringitis, ... | 1996 | 8959931 |
| route of infections in bovine aspergillosis. | repeated dna sequences were used to fingerprint strains of aspergillus fumigatus isolated from a cow with disseminated systemic aspergillosis, cows with single aspergillosis lesions, calves aborted due to bovine aspergillosis, mothers of those calves, and cattle without aspergillosis. the analysis of the southern blot hybridization patterns obtained suggested that: (i) the portal of entry of aspergillosis in cattle is the gastrointestinal tract, and (ii) infection of aborted calves is due to mat ... | 1996 | 8971626 |
| purification and characterization of a 93 kda aspergillus fumigatus antigen with diagnostic potential. | a glycoprotein with an apparent molecular weight of 93 kda was purified from a water-soluble extract of aspergillus fumigatus ncpf 2109 by single step affinity chromatography using the mannose-specific snowdrop (galanthus nivalis) lectin coupled to agarose. the carbohydrate moiety contained only mannose and galactose. partial sequencing of cyanogen bromide fragments of the antigen yielded two sequences, kqnkp and geipmkf?pql, with no homology to any reported proteins. in a preliminary evaluation ... | 1996 | 8971632 |
| expression and identification of a laminin-binding protein in aspergillus fumigatus conidia. | adhesion of aspergillus fumigatus, the causative agent of human aspergillosis, to the extracellular matrix protein laminin has been previously demonstrated. this study investigated the expression of laminin receptors during swelling of conidia, a step leading to germination and subsequent colonization of tissues. scanning electron microscopy showed that the laminin binding sites were distributed over the external rodlet layer of resting conidia. during swelling, the characteristic rodlet layer p ... | 1997 | 8975886 |
| il-10 exerts suppressive and enhancing effects on antifungal activity of mononuclear phagocytes against aspergillus fumigatus. | invasive aspergillosis has emerged as a frequent and serious infection in immunocompromised patients. we studied the effects of human il-10 on antifungal activity of monocytes (mncs) from healthy adults against aspergillus fumigatus after incubation with il-10 at 37 degrees c for 2 to 4 days. il-10 (2-20 ng/ml)-pretreated mncs exhibited approximately 40% suppression of superoxide anion (02-) production in response to pma and fmlp (p < or = 0.003), and anti-il-10 containing supernatant neutralize ... | 1997 | 8977206 |
| retroviral-mediated gene transfer of gp91phox into bone marrow cells rescues defect in host defense against aspergillus fumigatus in murine x-linked chronic granulomatous disease. | the x-linked form of chronic granulomatous disease (x-cgd), an inherited deficiency of the respiratory burst oxidase, results from mutations in the x-linked gene for gp91phox, the larger subunit of the oxidase cytochrome b. the goal of this study was to evaluate the impact of retroviral-mediated gene transfer of gp91phox on host defense against aspergillus fumigatus in a murine model of x-cgd. retrovirus vectors constructed using the murine stem cell virus (mscv) backbone were used for gene tran ... | 1997 | 8978275 |
| comparison of voriconazole (uk-109,496) and itraconazole in prevention and treatment of aspergillus fumigatus endocarditis in guinea pigs. | left-sided aspergillus fumigatus endocarditis was established in the guinea pig heart by catheterization and inoculation with conidia via a tributary of the femoral vein. this animal model was used to compare the efficacy of the triazole antifungal agents voriconazole (uk-109,496) and itraconazole. in the prophylaxis experiments, voriconazole at a dosage of 10 mg/kg of body weight given intraperitoneally twice daily prevented a. fumigatus endocarditis in all but 1 animal (11 of 12 animals were c ... | 1997 | 8980747 |
| t-8581, a new orally and parenterally active triazole antifungal agent: in vitro and in vivo evaluations. | t-8581 is a new water-soluble triazole antifungal agent. the geometric mean ic80s (gm-ic80s; where the ic80 is the lowest drug concentration which reduced the optical density at 630 nm by 80% compared with the optical density at 630 nm of the drug-free control) for candida albicans were as follows: t-8581, 0.218 microgram/ml; fluconazole; 0.148 microgram/ml; and itraconazole, 0.0170 microgram/ml. for cryptococcus neoformans the gm-ic80s were as follows: t-8581, 9.28 micrograms/ml; fluconazole, 4 ... | 1997 | 8980750 |
| pathology of acute aspergillosis in turkeys. | pathologic changes were characterized by gross examination and light microscopy after intra-air sac inoculation of 9- and 19-wk-old turkeys with aspergillus fumigatus conidia. turkeys were euthanatized and examined at 24, 48, 72, and 96 hr postinoculation (pi). lesions were largely confined to air sac membrane and lung tissues and were similar between the two age groups. there was progressive severity of gross lesions in both organs and of microscopic lesions in lung tissue. the character and se ... | 1996 | 8980820 |
| natural occurrence of gliotoxin in turkeys infected with aspergillus fumigatus, fresenius. | thirteen samples of infected turkey lung tissue from cases of 'airsacculitis' were collected either at the processing plant or from a local turkey farm and subjected to cultural and gliotoxin analysis. aspergillus fumigatus was isolated from 6 of the 13 samples; all isolates were determined to be gliotoxin producers when grown in laboratory culture and assayed by hplc procedures. gliotoxin was isolated from 5 of the 13 tissue but was not isolated from all tissues that were infected with a. fumig ... | 1996 | 8981782 |
| aspergillus fumigatus specific antibodies in multitransfused children with human immunodeficiency virus (hiv) infection in relation to serum levels of interleukin-2, gamma interferon and tumour necrosis factor. | anti-aspergillus fumigatus antibodies (igg and ige class) and serum levels of cytokines (gamma interferon, interleukin-2 and tumour necrosis factor-alpha) were studied in multitransfused (mt) children in relation to human immunodeficiency virus (hiv) infection. the specific antibodies to aspergillus fumigatus were present in 25 per cent of mt children seropositive for hiv as compared to only 2 per cent among hiv-negative mt children (x2 = 14, p < 0.001). estimation of serum cytokines level in mt ... | 1996 | 8984220 |
| binding and germination of aspergillus fumigatus conidia on cultured a549 pneumocytes. | invasive pulmonary aspergillosis is acquired through inhalation of conidia. little is known about early interactions between aspergillus fumigatus conidia and alveolar epithelial cells, so an in vitro model was developed to study binding between conidia and a549 cells, a line of type ii pneumocytes. conidia rapidly became attached to confluent monolayers of a549 cells in serum-free medium, reaching a plateau within 40 min. scanning electron microscopy (em) showed a random pattern of early adhere ... | 1997 | 8985209 |
| [invasive pulmonary aspergillosis caused by aspiration of polluted water after nearly drowning]. | a 21-year-old male nearly drowned in a reservoir by a traffic accident and he aspirated a large mount of polluted water containing aspergillus fumigatus. on admission to our hospital, he was alert but cyanotic. chest rentogenograms showed scatter infiltrative shadows in bilateral lung fields. we administered methylpredonizolon and antimicrobial agents immediately because of respiratory failure which was rapidly progressing. during the following six days, infiltrative shadows diminished but multi ... | 1996 | 8986073 |
| ruakuric acid: a natural product from aspergillus fumigatus. | a new chroman derivative, named ruakuric acid, was isolated from a strain of aspergillus fumigatus growing in conjunction with a coral lichen. the structure was determined as 6-acetyl-5-hydroxy-4-methoxy-chroman-2-carboxylic acid (mixture of 2,4-cis,trans isomers). | 1996 | 8987516 |
| pulmonary aspergillosis: a spectrum of disease. | although many species of the fungus aspergillus have been identified, the most common human pathogen is a. fumigatus, which has a worldwide distribution. although any organ may become infected, pulmonary aspergillosis is the most common manifestation. the spectrum of pulmonary aspergillosis includes saprophytic aspergillomas, allergic bronchopulmonary aspergillosis, chronic necrotizing aspergillosis, and invasive aspergillosis. immune status of the host, and the presence of underlying lung disea ... | 1996 | 8993947 |
| binding of aspergillus fumigatus spores to lung epithelial cells and basement membrane proteins: relevance to the asthmatic lung. | aspergillus fumigatus is an opportunistic pathogen to which asthmatic subjects are particularly susceptible. the ability of spores of a fumigatus to bind to pulmonary cells and basement membrane proteins was investigated to determine the mechanisms involved in this susceptibility. | 1996 | 8994516 |
| aspergillus fumigatus infection of an automatic internal cardiac defibrillator. | we report a patient without immune compromise with infection of an automatic internal cardiac defibrillator patch due to aspergillus fumigatus presenting 8 years after implantation. the mechanism of infection was unknown, but symptoms began 1 month after laser uvulopalatopharyngoplasty was performed for sleep apnea. the patches were surgically removed and the patient was treated sequentially with amphotericin b and itraconazole. he remains without evidence of infection 12 months after the comple ... | 1996 | 8994959 |
| functional endoscopic sinus surgery and 109 mycetomas of paranasal sinuses. | mycetomas of paranasal sinuses are more frequently diagnosed with the widespread use of nasal endoscopy and computed tomography (ct). we present a series of 109 cases treated by functional endoscopic sinus surgery (fess) with a mean follow-up of 29 months. all localizations were seen, and contrary to what was initially thought, seven cases presented in multiple sites. several clinical presentations were found, from a pansinusal involvement to a simple mycetoma hanging in a superior meatus. a het ... | 1997 | 9001274 |
| intensive care of patients with hiv infection: utilization, critical illnesses, and outcomes. pulmonary complications of hiv infection study group. | to examine intensive care unit (icu) admission rates and diagnoses of patients with hiv infection, and to determine the outcomes of different critical illnesses, we analyzed data derived from the 63 patients who were admitted to an icu from among the 1,130 adults with hiv infection who did not have aids at the time of enrollment in a multicenter prospective study. patients were admitted and treated according to the judgment of their physicians. during 4,298 patient-years of follow-up for the ent ... | 1997 | 9001291 |
| th1 and th2 cytokines in mice with invasive aspergillosis. | with a murine model of invasive aspergillosis we investigated cytokine production by cd4+ t helper cells and the effects of cytokine administration or neutralization on the course and outcome of infection. patterns of susceptibility and resistance to infection were obtained with different strains of mice injected with different inocula of aspergillus fumigatus conidia. mice surviving the primary infection also resisted a subsequent lethal infection that was associated with production of gamma in ... | 1997 | 9009314 |
| influence of zinc oxide on aspergillus species: a possible cause of local, non-invasive aspergillosis of the maxillary sinus. | recently the appearance of radiopaque 'concrements' in the maxillary sinus was reported. these radiodense objects could be identified as root-filling material for teeth of the upper jaw containing zinc oxide. this suggested that excess root-filling material containing zinc oxide in the maxillary sinus could favour the formation of a local, non-invasive aspergillosis. to verify this hypothesis in vitro, we tested the influence of zinc oxide on aspergillus fumigatus, a. flavus, a. terreus, a. nidu ... | 1996 | 9009659 |
| absence of respiratory burst in x-linked chronic granulomatous disease mice leads to abnormalities in both host defense and inflammatory response to aspergillus fumigatus. | mice with x-linked chronic granulomatous disease (cgd) generated by targeted disruption of the gp91phox subunit of the nadph-oxidase complex (x-cgd mice) were examined for their response to respiratory challenge with aspergillus fumigatus. this opportunistic fungal pathogen causes infection in cgd patients due to the deficient generation of neutrophil respiratory burst oxidants important for damaging a. fumigatus hyphae. alveolar macrophages from x-cgd mice were found to kill a. fumigatus conidi ... | 1997 | 9016870 |
| a synthetic form of tracheal antimicrobial peptide has both bactericidal and antifungal activities. | we have chemically synthesized tracheal antimicrobial peptide (tap) with 38 amino acids and examined efficacy of the peptide on various organisms. the synthetic peptide showed potent bactericidal effect on both gram positive and negative bacteria. the action of bactericidal effect was relatively quick and 99.9% of e. coli cells were killed within 90 minutes at a concentration of 2.5 micrograms/ml of tap. the peptide also showed antifungal activity against both mycelia (aspergillus fumigatus) and ... | 1996 | 9017361 |
| efficacy of prophylactic aerosol amphotericin b lipid complex in a rat model of pulmonary aspergillosis. | invasive pulmonary aspergillosis remains an important cause of morbidity and mortality among transplant recipients and patients receiving cancer chemotherapy. the lipid-associated formulation of amphotericin b (amb), amb lipid complex (ablc), was evaluated for its prophylactic efficacy when it was administered as an aerosol in a rat model of pulmonary aspergillosis. aerosol ablc (aero-ablc), in doses from 0.4 to 1.6 mg/kg of body weight given 2 days before infection, significantly delayed mortal ... | 1997 | 9021176 |
| case report: pathomorphology of an experimental disseminated aspergillus fumigatus infection in rabbits. | in following a formerly successful protocol designed to produce antibodies to a. fumigatus (fres) we observed a disseminated, lethal fungal infection in healthy, specific pathogen-free (spf) rabbits. the pathomorphological findings included multiple miliary to avenaceous whitish nodules in the livers and kidneys, mycotic mesencephalitis, nephritis, hepatitis, myocarditis, hemorrhagic enteritis, and splenitis. the hyphae were surrounded by necrosis, which also occurred in the liver without the hy ... | 1997 | 9025324 |
| development of murine monoclonal antibodies for the immunohistochemical diagnosis of systemic bovine aspergillosis. | murine monoclonal antibodies (mabs) against water-soluble somatic antigens (wssa) and the wall fraction (wf) from aspergillus fumigatus were produced by fusion of splenocytes from immunized balb/c mice with mouse myeloma x63-ag 8.653 cells. the supernatants of in vitro cultured hybridomas were initially screened for reactivity with the wssa and the wf from a. fumigatus and wssa of other fungi in an enzyme-linked immunosorbent assay (elisa). supernatants reacting only with a. fumigatus antigens w ... | 1996 | 9026084 |
| automated specific ige assay with recombinant allergens: evaluation of the recombinant aspergillus fumigatus allergen i in the pharmacia cap system. | we report the results of a study comparing the recombinant aspergillus fumigatus allergen 1 (rasp f i) to commercial a. fumigatus extracts in serological assays. pharmacia cap system and skin tests. | 1996 | 9027442 |
| purification and characterization of an endo-1,3-beta-glucanase from aspergillus fumigatus. | an endo-1,3-beta-glucanase was purified from a cell wall autolysate of aspergillus fumigatus. this beta-glucanase activity was associated with a glycosylated 74-kda protein. using a sensitive colorimetric assay and a high-performance anion-exchange chromatography with a pulsed electrochemical detector for product analysis, it was shown that the endoglucanase hydrolysed exclusively linear 1,3-beta-glucan chains, had an optimum ph of 7.0 and an optimum temperature of 60 degrees c. a substrate kine ... | 1997 | 9030754 |
| aspergillus in cytology specimens: a review of 45 specimens from 36 patients. | to assess the clinical significance associated with the identification of fungal elements consistent with aspergillus in cytology specimens. | 1997 | 9034732 |
| allergen-induced bronchial hyperreactivity and eosinophilic inflammation occur in the absence of ige in a mouse model of asthma. | in patients with asthma, elevations of ige correlate both with allergic inflammation of the airways and with bronchial hyperreactivity (bhr). several investigations, using mouse models of this disease, have indicated a central role for ige in the pathogenesis of the eosinophilic inflammation as well as in the obstructive airway physiology of bhr. some diagnostic studies and therapeutic strategies for asthma are based on the putative role of ige in asthma pathogenesis. here, we use mice with a nu ... | 1997 | 9037055 |
| aspergillus infection of the epiglottis in a hiv positive patient. | | 1996 | 9038641 |
| a painful knee in an immunocompromised patient. | | 1997 | 9039414 |
| the in-vivo activity of an antifungal antibiotic, benanomicin a, in comparison with amphotericin b and fluconazole. | the in-vivo antifungal activity of benanomicin a administered intravenously or subcutaneously was compared with that of amphotericin b and fluconazole using animal models of systemic infections with candida albicans, aspergillus fumigatus and cryptococcus neoformans. the efficacy of benanomicin a in c. albicans infection was more pronounced when administered in multiple doses than in a single dose. this was also true of fluconazole, but not of amphotericin b, which showed no difference between s ... | 1997 | 9044030 |
| biochemical and antigenic characterization of a new dipeptidyl-peptidase isolated from aspergillus fumigatus. | a novel dipeptidyl-peptidase (dpp v) was purified from the culture medium of aspergillus fumigatus. this is the first report of a secreted dipeptidyl-peptidase. the enzyme had a molecular mass of 88 kda and contained approximately 9 kda of n-linked carbohydrate. the expression and secretion of dipeptidyl-peptidase varied with the growth conditions; maximal intra- and extracellular levels were detected when the culture medium contained only proteins or protein hydrolysates in the absence of sugar ... | 1997 | 9045640 |
| detection of galactomannan and complement activation in the pregnant mouse during experimental systemic aspergillosis. | foeto-placental infections are obtained when pregnant mice are challenged intravenously with conidial suspensions of aspergillus fumigatus. this experimental model, which is used without any immuno-suppressive pretreatment, can be employed to screen for differences in foeto-placental infectivity of a. fumigatus strains when the number of colony-forming units (cfus) in conidial suspensions used for infection is from 1 x 10(5) to 1 x 10(6). in the foeto-placental unit, hyphal growth was initiated ... | 1996 | 9048873 |
| novel reduction and hydroxylation products formed by aspergillus fumigatus from reichstein's substance s. | fermentation of reichstein's substance s with aspergillus fumigatus (am-21) under aerobic conditions yielded 17 alpha, 21-dihydroxy-5 alpha-pregn-l-ene-3.20-dione. 17 alpha,20 alpha, 21-trihydroxy-5 alpha-pregn-l-en-3-one. 6 beta,17 alpha, 21-trihydroxypregn-4-en-3,20-dione. 15 beta,17 alpha,21-trihydroxy-5 alpha-pregnane-3,20-dione, and 15 beta, 17 alpha,20 alpha,21-tetrahydroxy-5 alpha-pregn-l-en-3-one. each microbial metabolite was characterized by spectroscopic methods, including 13c nmr che ... | 1997 | 9055385 |
| polymerase chain reaction amplification of asp f i and alkaline protease genes from fungus balls: clinical application in pulmonary aspergillosis. | asp fi(18 kda) and alkaline protease (33 kda) are the 2 major antigens which are derived from aspergillus (a.) fumigatus and have been implicated as possible virulence factors in the pathogenesis of aspergillus-induced diseases. we attempted to detect fragments of genes encoding both proteins from fungus balls obtained at surgery or autopsy by polymerase chain reaction (pcr) amplification and then used pcr to test clinical samples. frozen-stored fungus ball samples from a patient with acute myel ... | 1997 | 9058095 |
| identification and evaluation of a major cytotoxin of a. fumigatus. | aspergillus fumigatus is a highly pathogenic fungus causing a wide spectrum of diseases in immunocompromised as well as immunocompetent hosts. the present work was undertaken to evaluate the cytotoxic nature of fractionated antigens of a. fumigatus against the mammalian cell lines (j774, raw, cho and l929). an enriched protein antigenic fraction of a. fumigatus was subjected to con a sepharose and phenyl sepharose chromatography. antigenic fractions, conaub (cona unbound) and psc iii (fraction i ... | 1997 | 9059985 |
| molecular typing of aspergillus fumigatus strains by sequence-specific dna primer (ssdp) analysis. | a pcr typing method has been developed and tested to investigate the polymorphism of clinical strains of aspergillus fumigatus. firstly, the dna fragments from random amplified polymorphic dna (rapd) patterns of nine epidemiologically and geographically non-related monosporal strains of a. fumigatus were cloned and sequenced. the pairs of five sequence-specific dna primers (ssdp), characteristic of the 5' and 3' extremities of the rapd products, were then used in high stringency pcr to type 43 c ... | 1997 | 9061355 |
| postmortem analysis of invasive aspergillosis in a tertiary care hospital. | to estimate the incidence of fatal invasive aspergillosis in a 1500-bed tertiary-care hospital and to investigate the utility of laboratory diagnostic approaches, necropsy protocols and microbiological data from 1994 and 1995 were reviewed. among 694 necropsies from 1693 patients who died in these two years, 27 (4%) cases of invasive aspergillosis were identified. twelve cases of invasive aspergillosis were found after transplantation of solid organs, three after bone marrow transplantation, fou ... | 1997 | 9063664 |
| trends in immunotherapy of fungal infections. | fungal infections are the primary cause of mortality in patients with severely impaired host defense mechanisms, such as neutropenic patients with acute leukemia or those who have undergone bone marrow transplantation. in view of the unacceptably high mortality due to disseminated candidiasis, it is rational to focus on augmentation of host defense mechanisms in addition to conventional antifungal therapy. in vitro, a variety of immunomodulators, including tumor necrosis factor, interferon-g, an ... | 1997 | 9063674 |
| role of particulate antigens of aspergillus in murine eosinophilia. | allergic bronchopulmonary aspergillosis, a disabling hypersensitivity lung disease, results from inhalation of aspergillus fumigatus antigens present in contaminated environments. a murine model has been developed to understand the immune mechanism involved in allergic bronchopulmonary aspergillosis. we have investigated the immunoregulatory role of different physical forms of a.fumigatus antigens, such as a.fumigatus spores, soluble antigens. and soluble antigen coupled inert particles, in the ... | 1997 | 9066514 |
| [saprophytic and invasive pulmonary aspergillosis]. | the characteristics of chest x-ray films in 19 patients with saprophytic pulmonary aspergillosis secondary to acid-fast bacilli infection were reported. the saprophytic form, exemplified by the classic fungus ball or mycetoma, results from the growth of fungal mycelia within a pre-existing area of destroyed lung, typically a pre-existing cavity such as that resulting from tuberculosis. aspergillus fumigatus was detected in 52.6% of sputum cultures from 19 patients. aspergillus precipitin test wa ... | 1997 | 9071093 |
| cutaneous aspergillosis. | the authors present a patient with advanced acquired immunodeficiency syndrome who developed a cutaneous lesion under the transparent dressing of a venous catheter. histologic examination of the lesion revealed suppurative granulomatous inflammation in the dermis with numerous branching hyphae within the follicular infundibulum. a culture of the biopsy material grew aspergillus fumigatus. aspergillus must be considered in the differential diagnosis of cutaneous lesions in patients infected with ... | 1997 | 9071553 |
| a 62 year old diabetic with weight loss and lung lesions. | | 1997 | 9073017 |
| genetic diversity among isolates of aspergillus fumigatus in patients with cystic fibrosis. | strains of aspergillus fumigatus (n = 24) were isolated from the sputa of six patients with cystic fibrosis during periods from 3 to 11 months. the genetic polymorphisms of the strains were studied using the random amplified polymorphic dna (rapd) assay with three single oligonucleotides and pairwise combined primers. the analysis of rapd patterns resulted in 15 different rapd types. in four patients, the colonizing type changed, whereas in two others the same types were detected over periods be ... | 1997 | 9084119 |
| granulocyte colony-stimulating factor administered in vivo augments neutrophil-mediated activity against opportunistic fungal pathogens. | granulocyte colony-stimulating factor (g-csf) not only increases neutrophil (polymorphonuclear leukocyte, pmnl) production but also modulates pmnl biologic function. to assess the ability of g-csf administered in vivo to enhance pmnl activity against opportunistic fungal pathogens, the antifungal activity of pmnl obtained from normal human volunteers before and after g-csf administration was compared. in vivo, g-csf significantly enhanced pmnl-mediated killing of aspergillus fumigatus and rhizop ... | 1997 | 9086172 |
| interleukin-10 is a natural suppressor of cytokine production and inflammation in a murine model of allergic bronchopulmonary aspergillosis. | we have used interleukin-10 (il-10) gene knockout mice (il-10-/-) to examine the role of endogenous il-10 in allergic lung responses to aspergillus fumigatus ag. in vitro restimulated lung cells from sensitized il-10-/- mice produced exaggerated amounts of il-4, il-5, and interferon-gamma (ifn-gamma) compared with wild-type (wt) lung cells. in vivo, the significance of il-10 in regulating responses to repeated a. fumigatus inhalation was strikingly revealed in il-10-/- outbred mice that had a 50 ... | 1997 | 9091582 |
| automated serology with recombinant allergens. a feasibility study. | diagnosis of allergic conditions is based on skin prick- and/or intradermal tests and on serology. both methods make use of allergenic preparations and the results mainly depend on the quality of the substances applied and on the reactivity of the subjects. recombinant allergens can be produced as highly pure proteins. they may contribute to a significant improvement of the diagnosis of allergic diseases provided that the allergens became available in a pure, fully antigenic form suitable for ro ... | 1996 | 9095230 |
| antifungal activity of a new triterpenoid glycoside from pithecellobium racemosum (m.). | in a continuation of our search for novel antifungal compounds from higher plants, the standard extract of the bark of pithecellobium racemosum was found to have good activity against important aids-related opportunistic yeasts. | 1997 | 9098881 |
| esophageal intramural pseudodiverticulosis. | a 66-year-old man presented with severe chronic dysphagia and weight loss. a barium esophagogram revealed a proximal esophageal stricture and multiple pseudodiverticula. after death from aspiration pneumonia, a postmortem examination revealed extensive esophageal pseudodiverticulosis. clinical presentation, pathogenesis, diagnosis, and management of this unusual disorder are reviewed. | 1997 | 9101133 |
| [primary pulmonary aspergilloma presenting as an isolated nodular shadow]. | a 55-year-old man was admitted to our hospital because of an abnormal shadow at the right hilum on a chest radiograph. the chest radiograph and the chest ct scan showed a nodular showed in the right s6. there was no air-crescent sign in the shadow, but ct-guided percutaneous needle biopsy led to the diagnosis of pulmonary aspergillosis. anti-fungal agents (miconazole, fluconazole, and itraconazole) were given but the patient's condition did not improve. surgical resection was done and the postop ... | 1997 | 9103855 |
| [pulmonary aspergilloma with ring enhancement on chest ct scans]. | a 77-year-old man with hemoptysis was admitted to our hospital. a coin lesion in the right middle lung field was observed on a chest x-ray film. enhanced ct scans of the chest showed the coin lesion with clear ring enhancement. ring-enhanced mass shadow in cases of pulmonary diseases are very rare. the level of anti-aspergillus antibody in serum was high and the patient was given the diagnosis of aspergillosis. an upper lobectomy was done and aspergillus organisms were found. | 1997 | 9103864 |
| combined treatment with amphotericin-b and granulocyte transfusion from g-csf-stimulated donors in an aplastic patient with invasive aspergillosis undergoing bone marrow transplantation. | granulocyte transfusions from g-csf stimulated donors were added to standard anti-infective treatment in preparation for and during allogeneic bone marrow transplantation in a young man affected by very severe acute aplastic anemia and invasive aspergillosis. nine concentrates with a mean neutrophil content of 18.7 x 10(9)/l (2.6 x 10(8)/kg patient b.w.) were transfused before and after marrow infusion. an impressive clinical improvement was noticed after each granulocyte transfusion, although t ... | 1997 | 9107088 |
| serial monitoring of aspergillus antigen in the early diagnosis of invasive aspergillosis. preliminary investigations with two examples. | a recently developed sandwich elisa, which detects aspergillus galactomannan, was tested retrospectively in serial serum samples from an allogeneic bone marrow transplant recipient with proven invasive aspergillosis (patient 1) and another with suspected disease (patient 2). galactomannan was detected in the serum 4 and 28 days, respectively, before pulmonary infiltrates suggestive of fungal infection first became apparent on the chest x-ray. aspergillus was detected by elisa and pcr in bal flui ... | 1997 | 9108182 |
| aspergilloma formation in a pneumatocele associated with pneumocystis carinii pneumonia. | | 1997 | 9110096 |
| relapsing aspergillus bronchitis in a double lung transplant patient, successfully treated with a new oral antimycotic agent. | | 1997 | 9118736 |
| characterization of the aspergillus nidulans aspnd1 gene demonstrates that the aspnd1 antigen, which it encodes, and several aspergillus fumigatus immunodominant antigens belong to the same family. | for the first time, an immunodominant aspergillus nidulans antigen (aspnd1) consistently reactive with serum samples from aspergilloma patients has been purified and characterized, and its coding gene (aspnd1) has been cloned and sequenced. aspnd1 is a glycoprotein with four n-glycosidically-bound sugar chains (around 2.1 kda each) which are not necessary for reactivity with immune human sera. the polypeptide part is synthesized as a 277-amino-acid precursor of 30.6 kda that after cleavage of a ... | 1997 | 9119471 |
| internalization of aspergillus fumigatus conidia by epithelial and endothelial cells. | the internalization of conidia of the opportunistic fungus aspergillus fumigatus by primary cell cultures of nonprofessional phagocytes was investigated. this study is the first to show that a. fumigatus conidia were able to be engulfed by tracheal epithelial, alveolar type ii, and endothelial cells. | 1997 | 9119494 |
| aspergillus fumigatus chse: a gene related to chs3 of saccharomyces cerevisiae and important for hyphal growth and conidiophore development but not pathogenicity. | the aspergillus fumigatus chse (afchse) gene was isolated from an a. fumigatus dna library on the basis of hybridization to a segment of saccharomyces cerevisiae chs3 (scchs3). the amino acid sequence derived from afchse is 28% identical with scchs3 and 80% identical with the product of aspergillus nidulans chsd (anchsd). a mutant strain constructed by disruption of afchse has reduced levels of mycelial chitin, periodic swellings along the length of hyphae, and a block in conidiation that can be ... | 1997 | 9126623 |
| aspergillus endocarditis in a leukemia patient diagnosed by a pcr assay. | a patient in a blastic phase of chronic myelocytic leukemia developed multiple arterial emboli that originated from mitral valve vegetation. the diagnosis of infective endocarditis was not confirmed because blood cultures, serological assays and other examinations detected no pathogens. he died of intracranial hemorrhage after thrombolytic manipulation for embolization of the abdominal aorta and an autopsy was performed. polymerase chain reaction analysis and southern blot analysis of tissues fr ... | 1997 | 9128014 |
| dose-dependent enhancements by interferon-gamma on functional responses of neutrophils from chronic granulomatous disease patients. | interferon-gamma (ifn-gamma) is recommended as prophylaxis against infections in patients with chronic granulomatous disease (cgd). however, since the optimal dose, the dosing interval, and the mechanisms of action are not well-defined, we studied the effects on cgd neutrophil (pmn) functions ex vivo of interferon-gamma (ifn-gamma). evaluations were made on oxidative capacity, measured by superoxide anion production and chemiluminescence after stimulation with f-met-leu-phe (f-mlp) or phorbol-my ... | 1997 | 9129047 |
| cloning, production, characterization and ige cross-reactivity of different manganese superoxide dismutases in individuals sensitized to aspergillus fumigatus. | manganese superoxide dismutase (mnsod) from aspergillus fumigatus has been demonstrated to be an allergen showing a high degree of homology with phylogenetically distant mnsods. we describe cloning, production and characterization of mnsods from different species. | 1997 | 9130526 |
| molecular cloning and expression of amadoriase isoenzyme (fructosyl amine:oxygen oxidoreductase, ec 1.5.3) from aspergillus fumigatus. | amadoriase is an enzyme catalyzing the oxidative deglycation of amadori products to yield corresponding amino acids, glucosone, and h2o2. we previously reported the purification and characterization of two amadoriase isozymes from aspergillus sp. that degrade both glycated low molecular weight amines and amino acids (takahashi, m., pischetsrieder, m., and monnier, v. m. (1997) j. biol. chem. 272, 3437-3443). to identify the primary structure of the enzymes, we have prepared a cdna library from a ... | 1997 | 9139700 |
| clinical responses to ingested fungal alpha-amylase and hemicellulase in persons sensitized to aspergillus fumigatus? | alpha-amylase and hemicellulase, derived from culture of aspergillus species, are commonly added to flour as improvers during baking. two cases of women occupationally sensitized to alpha-amylase who developed allergic symptoms after eating baked bread have been reported. with a randomized, controlled study design, we have investigated whether similar responses occur in those sensitized to aspergillus species. seventeen subjects with positive skin prick tests to aspergillus fumigatus were studie ... | 1997 | 9140529 |
| gene cloning, purification, and characterization of a heat-stable phytase from the fungus aspergillus fumigatus. | the finding of heat-stable enzymes or the engineering of moderately thermostable enzymes into more stable ones by random or site-directed mutagenesis has become a main priority of modern biotechnology. we report here for the first time a heat-stable phytase able to withstand temperatures up to 100 degrees c over a period of 20 min, with a loss of only 10% of the initial enzymatic activity. the gene (phya) encoding this heat-stable enzyme has been cloned from aspergillus fumigatus and overexpress ... | 1997 | 9143104 |
| characterization of aspergillus isolates by pyrolysis mass spectrometry. | pyrolysis mass spectrometry (pyms) is a useful typing method for many bacterial and candida species. we attempted to type aspergillus spp. by pyms. four distinct a. fumigatus isolates could not be distinguished from each other, whereas one a. niger and one a. terreus could. poor reproducibility was shown using multiple identical cultures of a single a. fumigatus isolate and several isolates of the same dna type. pyms is obviously an unsuitable typing method for aspergillus spp. | 1996 | 9144999 |
| allergic fungal sinusitis: clinicopathological characteristics. | allergic fungal sinusitis is a comparatively new disease entity in paranasal sinus mycoses. it is not a very rare condition, but diagnosis is difficult to establish. of 28 consecutive cases of allergic nasal polyposis during a 2-year period, 11 patients had allergic fungal sinusitis and the diagnosis was based on the presence of type i hypersensitivity, eosinophilic mucus without tissue invasion of fungi on histopathology and detection of septate hyphae on direct microscopy. on culture, aspergil ... | 1996 | 9145000 |
| evaluation of himachalol in murine invasive aspergillosis. | himachalol (a sesquiterpene alcohol) showed low in vitro minimum inhibitory concentrations against aspergillus fumigatus by the macro- and microbroth dilution techniques (genometric mean 250 micrograms ml-1 and 46.4 micrograms ml-1 respectively) compared with saperconazole. swiss mice treated with the phytoproduct (200 mg kg-1, p.o.) once daily for 7 days exhibited a significant (p < 0.001) protection (60%) together with an increase in the mean survival time (15 days) and a reduced cfu (mean log ... | 1996 | 9145002 |
| prevalence of phthioic acid in aspergillus species. | we examined fast atom bombardment mass spectra (fab-ms) of 29 clinical isolates of aspergillus, from five pathogenic species, for the presence of phthioic acid anions (m/z 395.6) when grown at 37 degrees c. phthioic acid was detected in only one of 12 a. fumigatus, three of nine a. terreus and one of four a. niger isolates. phthioic acid is unlikely to be a major pathogenicity determinant of aspergillus. | 1997 | 9147275 |
| differential patterns of activity displayed by two exo-beta-1,3-glucanases associated with the aspergillus fumigatus cell wall. | two exo-beta-1,3-glucanases (herein designated exog-i and exog-ii) were isolated from the cell wall autolysate of the filamentous fungus aspergillus fumigatus and purified by ion-exchange, hydrophobic-interaction, and gel filtration chromatographies. molecular masses estimated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and gel filtration chromatography were 82 kda for the monomeric exog-i and 230 kda for the dimeric exog-ii. exog-i and exog-ii were glycosylated, and n glycans a ... | 1997 | 9150209 |
| two divergent catalase genes are differentially regulated during aspergillus nidulans development and oxidative stress. | catalases are ubiquitous hydrogen peroxide-detoxifying enzymes that are central to the cellular antioxidant response. of two catalase activities detected in the fungus aspergillus nidulans, the cata gene encodes the spore-specific catalase a (cata). here we characterize a second catalase gene, identified after probing a genomic library with cata, and demonstrate that it encodes catalase b. this gene, designated catb, predicts a 721-amino-acid polypeptide (catb) showing 78% identity to an aspergi ... | 1997 | 9150225 |
| aspergillus osteomyelitis after heart-lung transplantation. | aspergillus osteomyelitis is a severe complication of invasive aspergillosis. fewer than 15 cases have been observed after solid organ transplantation. we describe a case of aspergillus osteomyelitis of the ilium after heart-lung transplantation with favorable outcome after medical treatment. | 1997 | 9154954 |
| neosartorya fischeri: an invasive fungal pathogen in an allogeneic bone marrow transplant patient. | invasive fungal infections are a complication of allogeneic bmt. we report the first case of a neosartorya fischeri fungal infection in a patient following allogeneic bmt. neosartorya fischeri is related to aspergillus fumigatus, but it is a distinct fungal species. despite granulocytic engraftment and aggressive anti-fungal therapy with amphotericin b, the patient died of overwhelming fungal infection on day 60 post-bmt. neosartorya fischeri is a pathogen that grows slowly in culture which can ... | 1997 | 9156256 |
| [a nidulans bronchial aspergillosis after treatment of low-grade lymphoma with fludarabine]. | pulmonary aspergillosis is a common complication in neutropenic patients. the most important fungus is aspergillus fumigatus. we report a case of invasive pulmonary aspergillosis due to aspergillus nidulans secondary to fludarabine therapy. there are few cases of pulmonary aspergillosis due to aspergillus nidulans (one chronic necrotizing pulmonary aspergillosis, one aspergilloma). fludarabine induces a marked decreased of cd4 lymphocyte count responsible for opportunistic infections. it is the ... | 1997 | 9161576 |
| impaired host defense, hematopoiesis, granulomatous inflammation and type 1-type 2 cytokine balance in mice lacking cc chemokine receptor 1. | cc chemokine receptor 1 (ccr1) is expressed in neutrophils, monocytes, lymphocytes, and eosinophils, and binds the leukocyte chemoattractant and hematopoiesis regulator macrophage inflammatory protein (mip)-1alpha, as well as several related cc chemokines. four other ccr subtypes are known; their leukocyte and chemokine specificities overlap with, but are not identical to, ccr1, suggesting that ccr1 has both redundant and specific biologic roles. to test this, we have developed ccr1-deficient mi ... | 1997 | 9166425 |
| [allergic bronchopulmonary aspergillosis successfully treated with itraconazole]. | a 67-year-old man was admitted to our hospital because of coughing, a low-grade fever, and abnormal shadows on a chest x-ray film. he had had asthma as a child, but had no asthmatic symptoms on admission. a ct scan showed collapse of the right middle lobe and mucoid impactions in the lingula. bronchoscopy revealed thick mucus obstructing the right middle-lobe bronchus and the left upper-lobe bronchus. the eosinophil count and the ige level were abnormally high. aspergillus fumigatus was detected ... | 1997 | 9168655 |
| an antiangiogenic agent (tnp-470) inhibited reoxygenation during fractionated radiotherapy of murine mammary carcinoma. | tnp-470, a synthetic analogue of fumagillin which is a natural product of aspergillus fumigatus, has been noted as an angiogenesis inhibitor. combined effects of tnp-470 with fractionated radiotherapy (rt) were investigated using a mouse tumor. | 1997 | 9169820 |
| nmr study of the galactomannans of trichophyton mentagrophytes and trichophyton rubrum. | around 90% of chronic dermatophyte infections are caused by the fungi trichophyton mentagrophytes and trichophyton rubrum. one of the causes of the chronic infection resides in the immunosuppressive effects of the cell-wall components of these organisms. therefore we have attempted to identify the chemical structure of galactomannan, one of the major cell-wall components. the cell-wall polysaccharides secreted by t. mentagrophytes and t. rubrum were isolated from the culture medium and fractiona ... | 1997 | 9173896 |
| itraconazole resistance in aspergillus fumigatus. | invasive aspergillosis is an increasingly frequent opportunistic infection in immunocompromised patients. only two agents, amphotericin b and itraconazole, are licensed for therapy. itraconazole acts through inhibition of a p-450 enzyme undertaking sterol 14alpha demethylation. in vitro resistance in aspergillus fumigatus to itraconazole correlated with in vivo outcome has not been previously described. for three isolates (af72, af90, and af91) of a. fumigatus from two patients with invasive asp ... | 1997 | 9174200 |
| cytotoxic activity of ribonucleolytic toxin restrictocin-based chimeric toxins targeted to epidermal growth factor receptor. | targeted toxins represent a new approach to specific cytocidal therapy. the ribonucleolytic protein toxin restrictocin is a potent protein synthesis inhibitor produced by the fungus aspergillus restrictus. in the present study we have constructed two restrictocin based chimeric toxins where human transforming growth factor alpha (tgf alpha) has been used as a ligand. tgf alpha is a single chain polypeptide, which binds to epidermal growth factor receptor (egfr) and causes proliferation in a larg ... | 1997 | 9175867 |
| aspergillus fumigatus antigens: the pepys' years. | | 1997 | 9179439 |
| effects of synthetic form of tracheal antimicrobial peptide on respiratory pathogens. | we have synthesized a c-terminal portion of tracheal antimicrobial peptide (tap) with 38 amino acids and tested it for efficacy on various clinical isolates of pseudomonas aeruginosa strains from patients with cystic fibrosis and also on aspergillus fumigatus. our results indicate that the synthetic tap has both potent bactericidal and fungicidal activities and that a combination of tap and amphotericin b showed strong additive effects of growth inhibition on a fumigatus. these results suggest t ... | 1996 | 9182116 |
| stereoselective interaction of sch 39304, a triazole, with sterol 14alpha-demethylase of aspergillus fumigatus. | the inhibitory activity of sch 39304 and its enantiomers on radial growth and on the target enzyme, sterol 14alpha-demethylase, in aspergillus fumigatus was studied to assess the role of stereochemistry in the efficacy of the drug. sch 39304 and the rr(+) enantiomer were active in inhibiting the growth while no inhibition in the growth was observed with the ss(-) enantiomer. the mic of sch 39304 for the growth was about twice that of the rr(+) enantiomer. the differences in ic50s of sch 39304 an ... | 1997 | 9184358 |
| septic arthritis by aspergillus fumigatus: a complication of corticosteroid infiltration. | | 1997 | 9189072 |
| [microflora of the in furniture factors as a potential occupational hazard: concentration and composition of microflora and immunologic reactivity of workers to microbial aeroallergens]. | microbiologial studies of the air were performed in two furniture factories. the concentration of microorganisms in the air was low, being of the order 10(3) cfu/m3. the most common organisms were corynebacteria (arthrobacter, corynebacterium, brevibacterium, microbacterium) and fungi (aspergillus fumigatus, rhodotorula rubra). some of the species found in this environment possess known allergenic properties. allergological examinations of the workers with environmental aeroallergens have been p ... | 1996 | 9190236 |