| control of membrane-attached biofilms using surfactants. | the effect of surfactants on membrane-attached biofilms (mabs) was studied in a lab-scale extractive membrane bioreactor (emb). twenty-two surfactants were screened for their potential of increasing the cell wall negative charge (i.e. the electrostatic repulsion between bacteria) of burkholderia sp. js150 bacterial strain. surfactants resulting in increased bacterial negative charge were further investigated for their effects on mab population morphology and mab attachment behaviour. microscopic ... | 2006 | 16570318 |
| dural sinus thrombosis in melioidosis: the first case report. | melioidosis which is infection with burkholderia pseudomallei, is an important cause of sepsis in india, southeast asia and northern australia. mortality is high and treatment is problematic. neurological melioidosis is unusual but meningoencephalitis, encephalomyelitis and brain microabscess can occur dural sinus thrombosis is not an uncommon cerebrovascular disorder with various etiologies. hypercoagulable state, pregnancy, dehydration, certain blood dyscrasia and contraceptive pills are commo ... | 2006 | 16579013 |
| [bacteria of the genus burkholderia as a typical component of the microbial community of sphagnum peat bogs]. | bacteria of the genus burkholderia are a typical component of the microbial complex of sphagnum peat bogs and constitute a substantial portion of the aerobic chemoorganotrophic isolates which are routinely obtained from these environments on acidic nutrient media. the ecophysiological characteristics of the 27 strains of such organisms, which were isolated from the peat of acidic sphagnum bogs of the boreal and tundra zones of russia, canada, and estonia, were investigated in the present paper. ... | 2006 | 16579452 |
| a new green fluorescent protein-based bacterial biosensor for analysing phenanthrene fluxes. | the polycyclic aromatic hydrocarbon (pah)-degrading strain burkholderia sp. rp007 served as host strain for the design of a bacterial biosensor for the detection of phenanthrene. rp007 was transformed with a reporter plasmid containing a transcriptional fusion between the phns putative promoter/operator region and the gene encoding the enhanced green fluorescent protein (gfp). the resulting bacterial biosensor--burkholderia sp. strain rp037--produced significant amounts of gfp after batch incuba ... | 2006 | 16584481 |
| using real-time pcr to specifically detect burkholderia mallei. | burkholderia mallei is the causative agent of human and animal glanders and is a category b biothreat agent. rapid diagnosis of b. mallei and immediate prophylactic treatment are essential for patient survival. the majority of current bacteriological and immunological techniques for identifying b. mallei from clinical samples are time-consuming, and cross-reactivity with closely related organisms (i.e. burkholderia pseudomallei) is a problem. in this investigation, two b. mallei-specific real-ti ... | 2006 | 16585642 |
| isolation and identification of natural endophytic rhizobia from rice (oryza sativa l.) through rdna pcr-rflp and sequence analysis. | three novel endophytic rhizobial strains (rre3, rre5, and rre6) were isolated from naturally growing surface-sterilized rice roots. these isolates had the ability to nodulate common bean (phaseolus vulgaris). polymerase chain reaction-restriction fragment length polymorphism (pcr-rflp) and sequencing of 16s rdna of these isolates revealed that rre3 and rre5 are phylogenetically very close to burkholderia cepacia complex, whereas rre6 has affinity with rhizobium leguminosarum bv. phaseoli. plant ... | 2006 | 16586025 |
| a comparison of alternative 60-mer probe designs in an in-situ synthesized oligonucleotide microarray. | dna microarrays have proven powerful for functional genomics studies. several technologies exist for the generation of whole-genome arrays. it is well documented that 25mer probes directed against different regions of the same gene produce variable signal intensity values. however, the extent to which this is true for probes of greater length (60mers) is not well characterized. moreover, this information has not previously been reported for whole-genome arrays designed against bacteria, whose ge ... | 2006 | 16595014 |
| use of a variable amplicon typing scheme reveals considerable variation in the accessory genomes of isolates of burkholderia pseudomallei. | melioidosis, a disease caused by the bacterium burkholderia pseudomallei, is endemic in southeast asia and northern australia. we used suppression subtractive hybridization (ssh) to identify sequences that varied between two b. pseudomallei isolates from australia and determined the distribution of 45 ssh-derived sequences among a panel of b. pseudomallei and b. thailandensis isolates. sequences exhibiting variable prevalence were included in a variable amplicon typing (vat) scheme designed to s ... | 2006 | 16597858 |
| compliance of clinical microbiology laboratories in the united states with current recommendations for processing respiratory tract specimens from patients with cystic fibrosis. | respiratory tract specimens from patients with cystic fibrosis (cf) require unique processing by clinical microbiology laboratories to ensure detection of all potential pathogens. the present study sought to determine the compliance of microbiology laboratories in the united states with recently published recommendations for cf respiratory specimens. microbiology laboratory protocols from 150 of 190 (79%) cf care sites were reviewed. most described the use of selective media for burkholderia cep ... | 2006 | 16597890 |
| polychlorinated biphenyl (pcb)-degrading bacteria associated with trees in a pcb-contaminated site. | the abundance, identities, and degradation abilities of indigenous polychlorinated biphenyl (pcb)-degrading bacteria associated with five species of mature trees growing naturally in a contaminated site were investigated to identify plants that enhance the microbial pcb degradation potential in soil. culturable pcb degraders were associated with every plant species examined in both the rhizosphere and root zone, which was defined as the bulk soil in which the plant was rooted. significantly high ... | 2006 | 16597927 |
| degradation of aroclor 1242 dechlorination products in sediments by burkholderia xenovorans lb400(ohb) and rhodococcus sp. strain rha1(fcb). | burkholderia xenovorans strain lb400, which possesses the biphenyl pathway, was engineered to contain the oxygenolytic ortho dehalogenation (ohb) operon, allowing it to grow on 2-chlorobenzoate and to completely mineralize 2-chlorobiphenyl. a two-stage anaerobic/aerobic biotreatment process for aroclor 1242-contaminated sediment was simulated, and the degradation activities and genetic stabilities of lb400(ohb) and the previously constructed strain rha1(fcb), capable of growth on 4-chlorobenzoat ... | 2006 | 16597946 |
| identification of essential operons with a rhamnose-inducible promoter in burkholderia cenocepacia. | scanning of bacterial genomes to identify essential genes is of biological interest, for understanding the basic functions required for life, and of practical interest, for the identification of novel targets for new antimicrobial therapies. in particular, the lack of efficacious antimicrobial treatments for infections caused by the burkholderia cepacia complex is causing high morbidity and mortality of cystic fibrosis patients and of patients with nosocomial infections. here, we present a metho ... | 2006 | 16597956 |
| chlorophenol hydroxylases encoded by plasmid pjp4 differentially contribute to chlorophenoxyacetic acid degradation. | phenoxyalkanoic compounds are used worldwide as herbicides. cupriavidus necator jmp134(pjp4) catabolizes 2,4-dichlorophenoxyacetate (2,4-d) and 4-chloro-2-methylphenoxyacetate (mcpa), using tfd functions carried on plasmid pjp4. tfda cleaves the ether bonds of these herbicides to produce 2,4-dichlorophenol (2,4-dcp) and 4-chloro-2-methylphenol (mcp), respectively. these intermediates can be degraded by two chlorophenol hydroxylases encoded by the tfdb(i) and tfdb(ii) genes to produce the respect ... | 2006 | 16597983 |
| culturability of stream bacteria assessed at the assemblage and population levels. | lotic bacterial communities can be examined at multiple levels: from the assemblage level to populations of individual species. in stream environments, as in many other systems, the percentage of bacteria that are culturable is quite low. in this study, the culturability of the overall bacterial assemblage, as well as the culturability of three common species (acinetobacter calcoaceticus, burkholderia cepacia, and pseudomonas putida), was determined in samples collected from four streams on thre ... | 2006 | 16598638 |
| diverse bacteria associated with root nodules of spontaneous legumes in tunisia and first report for nifh-like gene within the genera microbacterium and starkeya. | we characterized 34 endophytic bacterial isolates associated to root nodules collected from spontaneous legumes in the arid zone of tunisia by 16s rdna polymerase chain reaction (pcr)-restriction fragment length polymorphism, whole cell protein sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page), 16s rdna and 16s-23s rdna internal transcribed spacer sequencing. phylogenetically, these isolates belong to the branches containing the genera inquilinus, bosea, rhodopseudomonas, para ... | 2006 | 16598639 |
| the burkholderia cepacia rpoe gene is not involved in exopolysaccharide production and onion pathogenicity. | burkholderia cepacia was originally described as the causative agent of bacterial rot of onions, and it has now emerged as an important opportunistic pathogen causing severe chronic lung infections in patients having cystic fibrosis. burkholderia cepacia is now classified into nine very closely related species (previously designated as genomovars), all of which have been isolated from both environmental and clinical sources and are collectively known as the b. cepacia complex. the alternative ex ... | 2006 | 16604122 |
| seroprevalence of burkholderia pseudomallei in east timorese refugees: implications for healthcare in east timor. | melioidosis is a disease with protean clinical manifestations caused by the bacterium burkholderia pseudomallei. it is endemic in countries surrounding the newly independent east timor, but has yet to be isolated or demonstrated serologically in that country. one illness that can be clinically indistinguishable from melioidosis is pulmonary tuberculosis, a condition with a very high prevalence in east timor. we used an indirect hemagglutination test (iha) to measure antibodies to b. pseudomallei ... | 2005 | 16610652 |
| roles for arg426 and trp111 in the modulation of nadh oxidase activity of the catalase-peroxidase katg from burkholderia pseudomallei inferred from ph-induced structural changes. | crystals of burkholderia pseudomallei katg retain their ability to diffract x-rays at high resolution after adjustment of the ph from 5.6 to 4.5, 6.5, 7.5, and 8.5, providing a unique view of the effect of ph on protein structure. one significant ph-sensitive change lies in the appearance of a perhydroxy group attached to the indole nitrogen of the active site trp111 above ph 7, similar to a modification originally observed in the ser324thr variant of the enzyme at ph 5.6. the modification forms ... | 2006 | 16618106 |
| genomic fingerprinting of burkholderia pseudomallei and b. mallei pathogens with dna array based on interspecies sequence differences obtained by subtractive hybridization. | the ability to rapidly and efficiently identify causative agents of dangerous human and animal diseases is a prerequisite to diagnosis, prophylaxis and therapy. such identification systems can be developed based on dna markers enabling differentiation between various bacterial strains. one source of these markers is genetic polymorphism. an efficient method for detecting the most stable polymorphisms without knowledge of genomic sequences is subtractive hybridization. in this work we report an a ... | 2006 | 16621455 |
| sustained endemicity of burkholderia cepacia complex in a pediatric institution, associated with contaminated ultrasound gel. | to determine whether contaminated ultrasound gel is the source of intermittent outbreaks of nosocomial infection due to burkholderia cepacia complex in patients without cystic fibrosis since 1992. | 2006 | 16622813 |
| a simple and rapid protein array based method for the simultaneous detection of biowarfare agents. | a protein chip has been developed that allows the simultaneous detection of a multitude of different biowarfare agents. the chip was developed for the arraytube platform providing a cheap and easy to handle technology solution that combines a microtube-integrated protein chip with the classical procedure of a sandwich-enzyme-linked immunosorbent assay and signal amplification by streptavidin-poly-horseradish peroxidase. specific immunoassays for staphylococcus enterotoxin b, ricin, venezuelan eq ... | 2006 | 16622830 |
| an improved selective and differential medium for the isolation of burkholderia pseudomallei from clinical specimens. | isolation and culture of burkholderia pseudomallei remains the main stay in the diagnosis of melioidosis. thus, the search for selective and differential media for b. pseudomallei has been ongoing. a number of such media have been reported with varying efficacy. ashdown medium is the most established selective medium for the isolation of b. pseudomallei. there are no reports of differential media differentiating b. pseudomallei from burkholderia cepacia. this report documents such a selective an ... | 2006 | 16626918 |
| phylogenetic study and multiplex pcr-based detection of burkholderia plantarii, burkholderia glumae and burkholderia gladioli using gyrb and rpod sequences. | in order to develop a detection method for the rice pathogens burkholderia plantarii, burkholderia glumae and burkholderia gladioli, the phylogeny of six plant-pathogenic burkholderia species was analysed using the combined nucleotide sequences of gyrb and rpod. b. plantarii, b. glumae and b. gladioli formed tight monophyletic branches supported by high bootstrap probabilities. the high sequence similarity revealed a close phylogenetic relationship between b. glumae and b. plantarii. b. plantari ... | 2006 | 16627650 |
| unrecognised infection in a cystic fibrosis patient. | we report a 17-year-old malay boy with cystic fibrosis who over a 14-month period experienced worsening respiratory symptoms and deteriorating lung function. burkholderia pseudomallei was eventually isolated from his sputum. he improved clinically following treatment for meliodosis and his lung function returned to normal. | 2006 | 16630326 |
| solution structure of monomeric bsal, the type iii secretion needle protein of burkholderia pseudomallei. | many gram-negative bacteria that are important human pathogens possess type iii secretion systems as part of their required virulence factor repertoire. during the establishment of infection, these pathogens coordinately assemble greater than 20 different proteins into a macromolecular structure that spans the bacterial inner and outer membranes and, in many respects, resembles and functions like a syringe. this type iii secretion apparatus (ttsa) is used to inject proteins into a host cell's me ... | 2006 | 16631790 |
| 7-o-malonyl macrolactin a, a new macrolactin antibiotic from bacillus subtilis active against methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of burkholderia cepacia. | we report here the discovery, isolation, and chemical and preliminary biological characterization of a new antibiotic compound, 7-o-malonyl macrolactin a (mma), produced by a bacillus subtilis soil isolate. mma is a bacteriostatic antibiotic that inhibits a number of multidrug-resistant gram-positive bacterial pathogens, including methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, and a small-colony variant of burkholderia cepacia. mma-treated staphylococci and entero ... | 2006 | 16641438 |
| differential display profiling of the nicotiana response to lps reveals elements of plant basal resistance. | the identification of cdnas, representing up-regulated genes induced by lipopolysaccharides from burkholderia cepacia, was achieved by differential display of mrnas isolated from tobacco cells. in addition to up-regulation of superoxide dismutase, involved in the production of the signalling and defense molecule, hydrogen peroxide; differentially expressed cdnas, indicative of the operation of an innate immune recognition system and expression of basal resistance, were identified. these include ... | 2006 | 16643858 |
| temocillin in the treatment of burkholderia cepacia infection in cystic fibrosis. | infections due to burkholderia cepacia complex (bcc) strains increase morbidity and mortality in cystic fibrosis (cf). some transplant centres reject bcc infected patients. we reviewed the results in patients treated with i.v temocillin. | 2006 | 16650744 |
| diazotrophic burkholderia species associated with field-grown maize and sugarcane. | until recently, diazotrophy was known in only one of the 30 formally described species of burkholderia. novel n(2)-fixing plant-associated burkholderia species such as b. unamae, b. tropica, and b. xenovorans have been described, but their environmental distribution is scarcely known. in the present study, the occurrence of n(2)-fixing burkholderia species associated with different varieties of sugarcane and maize growing in regions of mexico and brazil was analyzed. only 111 out of more than 90 ... | 2006 | 16672447 |
| the general secretory pathway of burkholderia gladioli pv. agaricicola bg164r is necessary for cavity disease in white button mushrooms. | cavity disease in white button mushrooms is caused by burkholderia gladioli pv. agaricicola. we describe the isolation and characterization of six mutants of the strain bg164r that no longer cause this disease on mushrooms. the mutations were mapped to genes of the general secretory pathway (gsp). this is the first report of the association of the type ii secretion pathway with a disease in mushrooms. phenotypes of the six avirulent mutants were the following: an inability to degrade mushroom ti ... | 2006 | 16672503 |
| the ornibactin biosynthesis and transport genes of burkholderia cenocepacia are regulated by an extracytoplasmic function sigma factor which is a part of the fur regulon. | burkholderia cenocepacia mutants that fail to produce the siderophore ornibactin were obtained following mutagenesis with mini-tn5tp. these mutants were shown to be growth restricted under conditions of iron depletion. in eight of the mutants, the transposon had integrated into one of two genes, orbi and orbj, encoding nonribosomal peptide synthetases. in the other mutant, the transposon had inserted into an open reading frame, orbs, located upstream from orbi. the polypeptide product of orbs ex ... | 2006 | 16672617 |
| an electronmicroscopic study of the interaction of burkholderia pseudomallei and human macrophages. | b. pseudomallei has been shown to persist intracellularly in melioidosis patients until reactivated by decreasing immunocompetence. we have shown by transmission electron microscopy the internalization of b. pseudomallei by human macrophages via conventional phagocytosis enclosed within membrane-bound vacuoles or phagosomes. ferritin labeled lysosomes provided evidence of phagosome-lysosome fusion. ingested bacilli were designated as "intact" or "damaged" on the basis of their ultrastructural fe ... | 2005 | 16676686 |
| involvement of quorum sensing and rpos in rice seedling blight caused by burkholderia plantarii. | burkholderia plantarii is a plant pathogen responsible for causing rice seedling blight. the molecular mechanisms responsible for this pathogenicity are currently unknown. in this study, we report the identification and characterization of n-acyl homoserine lactone quorum sensing and the stationary phase rpos sigma factor of b. plantarii. both global regulatory systems are involved in causing rice seedling blight. this is the first report of gene regulators of b. plantarii implicated in the dise ... | 2006 | 16684109 |
| application of a reca gene-based identification approach to the maize rhizosphere reveals novel diversity in burkholderia species. | burkholderia species are widely distributed in the natural environment. we evaluated the use of the reca gene in a cultivation-independent approach to examine the burkholderia diversity associated with the maize rhizosphere. two types of reca gene library were constructed, one with broad-specificity reca primers (bur1 and bur2) and a second from the products of nested pcrs using burkholderia-specific primers (bur3 and bur4). the broad-specificity primer set provided near full-length reca sequenc ... | 2006 | 16684112 |
| burkholderia cepacia complex species: health hazards and biotechnological potential. | the burkholderia cepacia complex is a group of nine closely related bacterial species that have useful properties in the natural environment as plant pest antagonists, plant growth promoters and degradative agents of toxic substances. because these species are human opportunistic pathogens, especially in cystic fibrosis patients, biotechnological applications that involve environmental releases have been severely restricted. recent progress in understanding the taxonomy, epidemiology and ecology ... | 2006 | 16684604 |
| [cellular fatty acid composition of the burkholderia mallei and burkholderia pseudomallei strains as generic feature of burkholderia]. | the cellular fatty acid compositions of studied two strains of burkholderia mallei and a strain of burkholderia pseudomallei are represented by saturated and monounsaturated straight chain fatty acids with 14-18 carbon atoms, cyclopropane fatty acids c(17 inverted delta) and c(19 inverted delta), and hydroxy acids 3-oh-c14:0, 2-oh-c(16:0), and 3-oh-c(16:0). the strain variation of cyclopropane and unsaturated fatty acid levels was observed. the cellular fatty acid spectra of studied bacteria did ... | 2006 | 16686216 |
| burkholderia cenocepacia utilizes ferritin as an iron source. | burkholderia cenocepacia is a member of the burkholderia cepacia complex, a group of genetically similar species that inhabit a number of environmental niches, including the lungs of patients with cystic fibrosis (cf). to colonize the lung, this bacterium requires a source of iron to satisfy its nutritional requirements for this important metal. because of the high potential for damage in lung tissue resulting from oxygen-iron interactions, this metal is sequestered by a number of mechanisms tha ... | 2006 | 16687582 |
| identification of burkholderia cepacia complex pathogens by rapid-cycle pcr with fluorescent hybridization probes. | members of the burkholderia cepacia complex are important bacterial pathogens in cystic fibrosis (cf) patients. the b. cepacia complex currently consists of nine genetic subgroups (genomovars) of different epidemiological relevance and possibly of different pathogenic potential in humans. in this study, a new approach was developed for the rapid identification of b. cepacia genomovar i, burkholderia multivorans (genomovar ii), burkholderia cenocepacia (lineage iii-a and iii-b), burkholderia stab ... | 2006 | 16687590 |
| phylogeny of the 1-aminocyclopropane-1-carboxylic acid deaminase-encoding gene acds in phytobeneficial and pathogenic proteobacteria and relation with strain biogeography. | deamination of the ethylene precursor 1-aminocyclopropane-1-carboxylic acid (acc) is a key plant-beneficial trait found in plant growth-promoting rhizobacteria (pgpr) and phytosymbiotic bacteria, but the diversity of the corresponding gene (acds) is poorly documented. here, acds sequences were obtained by screening putative acc deaminase sequences listed in databases, based on phylogenetic properties and key residues. in addition, acds was sought in 71 proteobacterial strains by pcr amplificatio ... | 2006 | 16689877 |
| spatial organisation of microbiota in quiescent adenoiditis and tonsillitis. | the reasons for recurrent adenotonsillitis are poorly understood. | 2007 | 16698947 |
| culture conditions for the production of an acidic exopolysaccharide by the nitrogen-fixing bacterium burkholderia tropica. | the endophytic diazotrophic bacterium burkholderia tropica, strain ppe8, produced copious amounts of exopolysaccharide (eps) on batch growth in liquid synthetic media containing mannitol and glutamate as carbon and nitrogen sources. the effect of various aeration regimes and carbon source concentrations on eps production was determined, as well as the effects of temperature and time of growth. the degree of aeration had a great influence on the yield of eps, in contrast with the c:n ratio of the ... | 2006 | 16699575 |
| phenotypic characterization of three clinical isolates of burkholderia pseudomallei in ceará, brazil. | burkholderia pseudomallei, the causative agent of melioidosis was found in a small cluster of cases in tejuçuoca, ceará, brazil. tests were carried out to determine its phenotypic characteristics: colony morphology on ashdown agar and macconkey agar, biochemical profile in conventional biochemical tests and api 20ne, arabinose assimilation and susceptibility testing by disk diffusion, comparing with data in the literature. this study confirms the presence of b. pseudomallei in brazil and describ ... | 2006 | 16699716 |
| burkholderia pseudomallei infections in finnish tourists injured by the december 2004 tsunami in thailand. | | 2005 | 16702634 |
| bacillus subtilis is a potential degrader of pyrene and benzo[a]pyrene. | polycyclic aromatic hydrocarbons (pahs) are a group of compounds that pose many health threats to human and animal life. they occur in nature as a result of incomplete combustion of organic matter, as well as from many anthropogenic sources including cigarette smoke and automobile exhaust. pahs have been reported to cause liver damage, red blood cell damage and a variety of cancers. because of this, methods to reduce the amount of pahs in the environment are continuously being sought. the purpos ... | 2005 | 16705827 |
| effect of reduced ph on inorganic polyphosphate accumulation by burkholderia cepacia complex isolates. | burkholderia cepacia complex (bcc) isolates causing pulmonary infection in cystic fibrosis (cf) patients grow within an acidic environment in the lung. as exposure to acid ph has been shown to increase intracellular inorganic polyphosphate (polyp) formation in some bacteria, we investigated the inter-relationship between acidic ph and polyp accumulation in bcc isolates. | 2006 | 16706902 |
| isolation and characterization of a novel burkholderia cepacia with strong antifungal activity against rhizoctonia solani. | strain cf-66 with strong antifungal activity against rhizoctonia solani was isolated from compost samples. it is clearly demonstrated that strain cf-66 is belonging to burkholderia cepacia complex by the morphological and biochemical tests and 16s rdna sequence. the b. cepacia complex consists of a group of bacteria currently organized into nine genomovars, among them genomovar ii and genomovar iii, contain the highly epidemic strains. however, it was known that strain cf-66 is not a member of g ... | 2006 | 16708194 |
| confirmation of e. coli among other thermotolerant coliform bacteria in paper mill effluents, wood chips screening rejects and paper sludges. | paper sludges are solid wastes material generated from the paper production, which have been characterized for their chemical contents. some are rich in wood fiber and are a good carbon source, for example the primary and de-inking paper sludges. others are made rich in nitrogen and phosphorus by pressing the activated sludge, resulting from the biological water treatments, with the primary sludge, yielding the combined paper sludge. still, in the absence of sanitary effluents very few studies h ... | 2006 | 16714043 |
| identification of burkholderia pseudomallei genes required for the intracellular life cycle and in vivo virulence. | the bacterial pathogen burkholderia pseudomallei invades host cells, escapes from endocytic vesicles, multiplies intracellularly, and induces the formation of actin tails and membrane protrusions, leading to direct cell-to-cell spreading. this study was aimed at the identification of b. pseudomallei genes responsible for the different steps of this intracellular life cycle. b. pseudomallei transposon mutants were screened for a reduced ability to form plaques on ptk2 cell monolayers as a result ... | 2006 | 16714590 |
| different iron-chelating properties of pyochelin diastereoisomers revealed by lc/ms. | pyochelin is a siderophore and virulence factor common to burkholderia cepacia and several pseudomonas strains. it is isolated from bacterial media as a mixture of two epimers, which readily equilibrate in most solvents. experiments based on high-performance liquid chromatography/electrospray ionization mass spectrometry are reported here, allowing the investigation of the different fe(iii)-chelating properties of pyochelin diastereomers in solution without the need for labourious isolation. it ... | 2006 | 16715280 |
| isolation of fenitrothion-degrading strain burkholderia sp. fds-1 and cloning of mpd gene. | a short rod shaped, gram-negative bacterium strain burkholderia sp. fds-1 was isolated from the sludge of the wastewater treating system of an organophosphorus pesticides manufacturer. the isolate was capable of using fenitrothion as the sole carbon source for its growth. fds-1 first hydrolyzed fenitrothion to 3-methyl-4-nitrophenol, which was further metabolized to nitrite and methylhydroquinone. the addition of other carbon source and omitting phosphorus source had little effect on the hydroly ... | 2006 | 16715406 |
| localized attraction correlates with bacterial adhesion to glass and metal oxide substrata. | bacterial adhesion to surfaces does not always proceed according to theoretical expectations. discrepancies are often attributed to surface heterogeneities that provide localized, favorable sites for bacterial attachment. the presence of these favorable deposition sites for bacteria, however, has never been directly measured. atomic force microscopy (afm) was used to quantify the distribution of attractive sites on clean substrata. surfaces of silica and three different metal oxides mapped by ad ... | 2006 | 16719101 |
| genomic patterns of pathogen evolution revealed by comparison of burkholderia pseudomallei, the causative agent of melioidosis, to avirulent burkholderia thailandensis. | the gram-negative bacterium burkholderia pseudomallei (bp) is the causative agent of the human disease melioidosis. to understand the evolutionary mechanisms contributing to bp virulence, we performed a comparative genomic analysis of bp k96243 and b. thailandensis (bt) e264, a closely related but avirulent relative. | 2006 | 16725056 |
| resistance in nonfermenting gram-negative bacteria: multidrug resistance to the maximum. | nonfermenting gram-negative bacteria pose a particular difficulty for the healthcare community because they represent the problem of multidrug resistance to the maximum. important members of the group in the united states include pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, and burkholderia cepacia. these organisms are niche pathogens that primarily cause opportunistic healthcare-associated infections in patients who are critically ill or immunocompromised. mult ... | 2006 | 16735148 |
| enzymatic modification of cassava starch by bacterial lipase. | enzymatic modification of starch using long chain fatty acid makes it thermoplastic suitable for a myriad of industrial applications. an industrial lipase preparation produced by burkholderia cepacia (lipase ps) was used for modification of cassava starch with two acyl donors, lauric acid and palmitic acid. reactions performed with palmitic acid by liquid-state and microwave esterification gave a degree of substitution (ds) of 62.08% (ds 1.45) and 42.06% (ds 0.98), respectively. thermogravimetri ... | 2006 | 16736230 |
| use of ribosomal promoters from burkholderia cenocepacia and burkholderia cepacia for improved expression of transporter protein in escherichia coli. | expression of heterologous protein in escherichia coli usually based on the iptg-inducible expression systems. the use of these systems for membrane protein production, however, usually caused cytotoxic problem that affected the yield and functional characterization of the protein. optimization of these systems for transporter protein production is time-consuming and is usually ineffective. here, we described the use of the ribosomal promoters p(s12) from burkholderia cenocepacia lmg16656 and fr ... | 2006 | 16737826 |
| cystic fibrosis as a risk factor for recurrent venous thrombosis at a pediatric tertiary care hospital. | to evaluate risk factors for recurrent thrombosis in pediatric patients. | 2006 | 16737881 |
| proteome changes after metabolic engineering to enhance aerobic mineralization of cis-1,2-dichloroethylene. | metabolically engineered escherichia coli has previously been used to degrade cis-1,2-dichloroethylene (cis-dce). the strains express the six genes of an evolved toluene ortho-monooxygenase from burkholderia cepacia g4 (tom-green, which formed a reactive epoxide) with either (1) gamma-glutamylcysteine synthetase (gshi, which forms glutathione) and the glutathione s-transferase isoilr1 from rhodococcus ad45 (which adds glutathione to the reactive cis-dce epoxide) or (2) with an evolved epoxide hy ... | 2006 | 16739990 |
| a glutathione s-transferase catalyzes the dehalogenation of inhibitory metabolites of polychlorinated biphenyls. | bphk is a glutathione s-transferase of unclear physiological function that occurs in some bacterial biphenyl catabolic (bph) pathways. we demonstrated that bphk of burkholderia xenovorans strain lb400 catalyzes the dehalogenation of 3-chloro 2-hydroxy-6-oxo-6-phenyl-2,4-dienoates (hopdas), compounds that are produced by the cometabolism of polychlorinated biphenyls (pcbs) by the bph pathway and that inhibit the pathway's hydrolase. a one-column protocol was developed to purify heterologously pro ... | 2006 | 16740949 |
| protein engineering of the 4-methyl-5-nitrocatechol monooxygenase from burkholderia sp. strain dnt for enhanced degradation of nitroaromatics. | 4-methyl-5-nitrocatechol (4m5nc) monooxygenase (dntb) from burkholderia sp. strain dnt catalyzes the second step of 2,4-dinitrotoluene degradation by converting 4m5nc to 2-hydroxy-5-methylquinone with the concomitant removal of the nitro group. dntb is a flavoprotein that has a very narrow substrate range. here, error-prone pcr was used to create variant dntb m22l/l380i, which accepts the two new substrates 4-nitrophenol (4np) and 3-methyl-4-nitrophenol (3m4np). at 300 microm of 4np, the initial ... | 2006 | 16751499 |
| [melioidosis-like disease: infection with burkholderia cocovenenans as rare differential diagnosis for lymphadenitis colli]. | neoplasms, autimmune disorders and infectious diseases as differential diagnoses of cervical lymphadenopathies also require the consideration of rare causes. | 2007 | 16755460 |
| role of selective and nonselective media for isolation of burkholderia pseudomallei from throat swabs of patients with melioidosis. | | 2006 | 16757651 |
| development of antibodies to burkholderia pseudomallei during childhood in melioidosis-endemic northeast thailand. | a cross-sectional serological survey of 2,214 children living in northeast thailand was conducted to define the antibody response to burkholderia pseudomallei from birth to 14 years. there was a sharp rise in detectable antibodies from birth to 4 years followed by reactivity in approximately 60-70% of children thereafter. | 2006 | 16760522 |
| polyhydroxyalkanoate copolymers from forest biomass. | the potential for the use of woody biomass in poly-beta-hydroxyalkanoate (pha) biosynthesis is reviewed. based on previously cited work indicating incorporation of xylose or levulinic acid (la) into phas by several bacterial strains, we have initiated a study for exploring bioconversion of forest resources to technically relevant copolymers. initially, pha was synthesized in shake-flask cultures of burkholderia cepacia grown on 2.2% (w/v) xylose, periodically amended with varying concentrations ... | 2006 | 16761168 |
| polysaccharide microarray technology for the detection of burkholderia pseudomallei and burkholderia mallei antibodies. | a polysaccharide microarray platform was prepared by immobilizing burkholderia pseudomallei and burkholderia mallei polysaccharides. this polysaccharide array was tested with success for detecting b. pseudomallei and b. mallei serum (human and animal) antibodies. the advantages of this microarray technology over the current serodiagnosis of the above bacterial infections were discussed. | 2006 | 16765554 |
| a bioconjugate of pseudomonas cepacia lipase with alginate with enhanced catalytic efficiency. | a bioconjugate of pseudomonas cepacia lipase with alginate was prepared by simple adsorption. atomic force microscope (afm) images showed that this bioconjugate resulted from adsorption rather than entrapment of the enzyme as enzyme molecules were visible on the gel surface. the soluble bioconjugate exhibited increased enzyme activity in terms of high effectiveness factor (effectiveness factor was 3 for the immobilized preparation) and greater vmax/km value (vmax/km increased 25 times upon immob ... | 2006 | 16765657 |
| isolation of bacterial antagonists of aspergillus flavus from almonds. | bacteria were isolated from california almond orchard samples to evaluate their potential antifungal activity against aflatoxin-producing aspergillus flavus. fungal populations from the same samples were examined to determine the incidence of aflatoxigenic aspergillus species. antagonistic activities of the isolated bacterial strains were screened against a nonaflatoxigenic nor mutant of a. flavus, which accumulates the pigmented aflatoxin precursor norsolorinic acid (nor) under conditions condu ... | 2006 | 16767519 |
| management of melioidosis. | melioidosis is a serious human infection caused by the environmental gram-negative bacterium burkholderia pseudomallei. outcome following melioidosis remains poor despite 20 years of clinical research. overall mortality is 50% in north-east thailand (35% in children) and 19% in australia. relapse is common (13% over 10 years), and results from failure to eradicate the organism. treatment is required to complete 12-20 weeks, or longer if clinically indicated. this is divided into intravenous and ... | 2006 | 16771621 |
| caenorhabditis elegans killing assay as an infection model to study the role of type iii secretion in burkholderia cenocepacia. | | 2006 | 16772429 |
| suppurative mediastinitis secondary to burkholderia cepacia in a patient with cystic fibrosis. | burkholderia cepacia is an important opportunistic pathogen among patients with cystic fibrosis (cf); it is associated with deterioration of lung function, poor outcome following lung transplantation and increased mortality. fever, an elevated white blood cell count, weight loss and an often fatal deterioration in pulmonary function characterize a particular clinical course, termed "cepacia syndrome". the present case report describes a 40-year-old man with cf who developed cepacia syndrome comp ... | 2006 | 16779467 |
| is a honeycomb appearance on computer tomography characteristic for burkholderia pseudomallei liver abscess? | | 2006 | 16779761 |
| invasion and biofilm formation of burkholderia dolosa is comparable with burkholderia cenocepacia and burkholderia multivorans. | colonisation with burkholderia cepacia complex pathogens has been associated with accelerated decline in cystic fibrosis (cf) patients. the two most common species among the cf community are burkholderia cenocepacia and burkholderia multivorans. however, burkholderia dolosa has recently been causing concern due to its transmissibility and virulence in cf patients. | 2007 | 16781896 |
| in vitro antimicrobial activity of natural toxins and animal venoms tested against burkholderia pseudomallei. | burkholderia pseudomallei are the causative agent of melioidosis. increasing resistance of the disease to antibiotics is a severe problem in treatment regime and has led to intensification of the search for new drugs. antimicrobial peptides are the most ubiquitous in nature as part of the innate immune system and host defense mechanism. | 2006 | 16784542 |
| life-threatening sepsis caused by burkholderia cepacia from contaminated intravenous flush solutions prepared by a compounding pharmacy in another state. | we report 2 life-threatening cases of burkholderia cepacia sepsis caused by infusate contamination during compounding. bacterial isolates from the patients' blood cultures and the infusate were indistinguishable by pulsed-field gel electrophoresis. proper quality controls at a local and national level are important for ensuring safe delivery of compounded medications to patients in all settings, including those outside health care facilities. | 2006 | 16785290 |
| molecular cloning and expression of perhydrolase genes from pseudomonas aeruginosa and burkholderia cepacia in escherichia coli. | two bacterial perhydrolase genes, perpa and perbc, were cloned from pseudomonas aeruginosa and burkholderia cepacia, respectively, using pcr amplification with primers designed to be specific for conserved amino acid sequences of the already-known perhydrolases. the amino acid sequence of perpa was identical to a putative perhydrolase of p. aeruginosa pao1 genome sequences, whereas perbc of b. cepacia was a novel bacterial perhydrolase showing similarity of less than 80% with all other existing ... | 2006 | 16786268 |
| spontaneous septic arthritis caused by burkholderia cepacia. | we describe the first reported case of a spontaneous septic arthritis caused by burkholderia cepacia, the organism responsible for onion skin rot. the source of infection was most likely from hematogenous spread, as the patient's blood cultures were positive for b. cepacia. treatment involved arthroscopic irrigation and drainage of the affected shoulder. despite post-operative resolution of this immunocompromised patient's shoulder symptoms, he was unable to survive the b. cepacia bacteremia. ou ... | 2006 | 16789467 |
| burkholderia cenocepacia zmpb is a broad-specificity zinc metalloprotease involved in virulence. | in previous studies we characterized the burkholderia cenocepacia zmpa zinc metalloprotease. in this study, we determined that b. cenocepacia has an additional metalloprotease, which we designated zmpb. the zmpb gene is present in the same species as zmpa and was detected in b. cepacia, b. cenocepacia, b. stabilis, b. ambifaria, and b. pyrrocinia but was absent from b. multivorans, b. vietnamiensis, b. dolosa, and b. anthina. the zmpb gene was expressed, and zmpb was purified from escherichia co ... | 2006 | 16790782 |
| the animal pathogen-like type iii secretion system is required for the intracellular survival of burkholderia mallei within j774.2 macrophages. | burkholderia mallei is a highly infectious gram-negative pathogen and is the causative agent of human and animal glanders. by generating polar mutations (disruption of bsaq and bsaz) in the b. mallei atcc 23344 animal pathogen-like type iii secretion system (tts), we demonstrate that this bacterial protein delivery system is required for intracellular growth of b. mallei in j774.2 cells, formation of macrophage membrane protrusions, actin polymerization, and phagosomal escape. these findings sug ... | 2006 | 16790809 |
| on-line low-volume transesterification-based assay for immobilized lipases. | a method has been developed for fast evaluation of transesterification activity of immobilized lipases using microlitre and submicrolitre volumes of substrate solutions. the model reaction (acylation of isopropanol with vinyl acetate) is catalyzed by microbial lipases immobilized on ceramic particles, diatoms or acrylic resin, packed into a teflon tube connected to fused-silica capillary tubing. the substrate solution is pumped through the microreactor and the product of transesterification, ace ... | 2006 | 16793159 |
| identification of a response regulator gene for catabolite control from a pcb-degrading beta-proteobacteria, acidovorax sp. kks102. | acidovorax sp. (formally pseudomonas sp.) strain kks102 carries a bph operon for the degradation of pcb/biphenyl. transcription from the pe promoter for the bph operon was found to be under catabolite control, i.e. the promoter activity was at a lower level when succinate, fumarate or acetate was added to the culture. some mutations in the immediate upstream region of the pe promoter resulted in catabolite-insensitive and constitutively low promoter activity, suggesting that a transcriptional ac ... | 2006 | 16796688 |
| clinical variation in melioidosis in pigs with clonal infection following possible environmental contamination from bore water. | an outbreak of melioidosis occurred in pigs on a rural property in the tropical north of the northern territory of australia. the pigs were mostly asymptomatic but lesions in the parotid glands suggested an oral route of infection. skin lesions were also common and one piglet had disseminated infection. pulsed-field gel electrophoresis showed an identical pattern amongst burkholderia pseudomallei isolates from the pigs, with similarity to an isolate from the unchlorinated bore water supplying th ... | 2007 | 16807011 |
| enzymatic approach to enantiomerically pure 5-alken-2,4-diols and 4-hydroxy-5-alken-2-ones: application to the synthesis of chiral synthons. | enantiomerically pure 1,3-diols 1-3 were obtained by a chemoenzymatic approach (lipase ps from burkholderia cepacia). these diols were converted into useful chiral synthons, which could be considered homologues of glyceraldehyde and glyceric acid acetonides. applications of these synthons to the de novo synthesis of sugars and preparation of conagenin carboxylic moiety were shown. hydroxy ketone 4 was chosen as a model system for another synthetic evolution: it was obtained in enantiomerically p ... | 2006 | 16808510 |
| resistance in nonfermenting gram-negative bacteria: multidrug resistance to the maximum. | nonfermenting gram-negative bacteria pose a particular difficulty for the healthcare community because they represent the problem of multidrug resistance to the maximum. important members of the group in the united states include pseudomonas aeruginosa, acinetobacter baumannii, stenotrophomonas maltophilia, and burkholderia cepacia. these organisms are niche pathogens that primarily cause opportunistic healthcare-associated infections in patients who are critically ill or immunocompromised. mult ... | 2006 | 16813979 |
| impacts of 2,4-d application on soil microbial community structure and on populations associated with 2,4-d degradation. | the effect of 2,4-dichlorophenoxyacetic acid (2,4-d) application rate on microbial community structure and on the diversity of dominant 2,4-d degrading bacteria in an agricultural soil was examined using cultivation-independent molecular techniques coupled with traditional isolation and enumeration methods. fingerprints of microbial communities established under increasing concentrations of 2,4-d (0-500 mg kg-1) in batch soil microcosms were obtained using denaturing gradient gel electrophoresis ... | 2007 | 16814534 |
| spatially-correlated mass spectrometric analysis of microbe--mineral interactions. | a new methodology for examining the interactions of microbes with heterogeneous minerals is presented. imaging laser desorption fourier transform mass spectrometry was used to examine the colonization patterns of burkholderia vietnamiensis g4 (previously burkholderia cepacia g4) on a heterogeneous basalt sample. depth-profile imaging found that the bacterium preferentially colonized the plagioclase mineral phase within the basalt. | 2006 | 16815575 |
| coexistence in the chemostat as a result of metabolic by-products. | classical chemostat models assume that competition is purely exploitative and mediated via a common, limiting and single resource. however, in laboratory experiments with pathogens related to the genetic disease cystic fibrosis, species specific properties of production, inhibition and consumption of a metabolic by-product, acetate, were found. these assumptions were implemented into a mathematical chemostat model which consists of four nonlinear ordinary differential equations describing two sp ... | 2006 | 16819650 |
| a bacterium belonging to the burkholderia cepacia complex associated with pleurotus ostreatus. | pleurotus ostreatus is a widely cultivated white-rot fungus. owing to its considerable enzymatic versatility p. ostreatus has become the focus of increasing attention for its possible utility in biobleaching and bioremediation applications. interactions between microorganisms can be an important factor in those processes. in this study, we describe the presence of a bacterial species associated with p. ostreatus strain g2. this bacterial species grew slowly (approximately 30 days) in the liquid ... | 2006 | 16820755 |
| evaluation of pyrrolidonyl arylamidase for the identification of nonfermenting gram-negative rods. | to evaluate the activity of pyrrolidonyl arylamidase (pyr) for the differentiation and identification of nonfermenting gram negative rods (nfgnr), 293 isolates were tested. a 24 h culture of each test organism was prepared. from this a 108-109 cfu/ml suspension was added to 0.25 ml of sterile physiologic solution. a pyr disk was then added and the test was incubated for 30 minutes at 35-37 degrees c, at environmental atmosphere. reading was done by adding 1 drop of cinnamaldehyde reagent. strain ... | 2007 | 16822636 |
| nonrandom distribution of burkholderia pseudomallei clones in relation to geographical location and virulence. | burkholderia pseudomallei is a soil-dwelling saprophyte and the causative agent of melioidosis, a life-threatening human infection. most cases are reported from northeast thailand and northern australia. using multilocus sequence typing (mlst), we have compared (i) soil and invasive isolates from northeast thailand and (ii) invasive isolates from thailand and australia. a total of 266 thai b. pseudomallei isolates were characterized (83 soil and 183 invasive). these corresponded to 123 sequence ... | 2006 | 16825379 |
| 1h and 13c assignments of cyclo[n-(lys-phe)-orn-val], a semicyclic imide tetrapeptide from burkholderia cepacia. | the rhizobacteria burkholderia cepacia biosynthesized the new tetrapeptide cyclo[n-(lys-phe)-orn-val] (1), a 2,5-diketopiperazine, and the known siderophore azurechelin (salicylic acid). the structure of 1 was established by means of ir, uv, 1h and 13c nmr, double dimension experiments and ms. | 2006 | 16826554 |
| actin-based motility of intracellular bacteria, and polarized surface distribution of the bacterial effector molecules. | several intracellular bacterial pathogens, including species of listeria, rickettsia, shigella, mycobacteria, and burkholderia, have evolved mechanisms to exploit the actin polymerization machinery of their hosts to induce actin-based motility, enabling these pathogens to spread between host cells without exposing themselves to the extracellular milieu. efficient cell-to-cell spread requires directional motility, which the bacteria may achieve by concentrating the effector molecules at one pole ... | 2006 | 16826602 |
| isolation measures for prevention of infection with respiratory pathogens in cystic fibrosis: a systematic review. | respiratory infections are the most important cause of morbidity and mortality in patients with cystic fibrosis (cf). these infections are typically caused by a limited number of respiratory pathogens, particularly burkholderia cepacia complex (bcc) and pseudomonas aeruginosa (pa). since the 1980s, several outbreaks of unique strains of pa and bcc among cf patients attending the same cf care centres have been described, leading to a sharp decline in the patients' health. one of the measures adop ... | 2006 | 16835001 |
| a novel alpha-ketoglutaric semialdehyde dehydrogenase: evolutionary insight into an alternative pathway of bacterial l-arabinose metabolism. | azospirillum brasilense possesses an alternative pathway of l-arabinose metabolism, which is different from the known bacterial and fungal pathways. in a previous paper (watanabe, s., kodaki, t., and makino, k. (2006) j. biol. chem. 281, 2612-2623), we identified and characterized l-arabinose 1-dehydrogenase, which catalyzes the first reaction step in this pathway, and we cloned the corresponding gene. here we focused on the fifth enzyme, alpha-ketoglutaric semialdehyde (alphakgsa) dehydrogenase ... | 2006 | 16835232 |
| doripenem. | doripenem is a new member of the carbapenem class of beta-lactam antibiotics with broad-spectrum coverage of gram-positive, gram-negative and anaerobic pathogens similar to imipenem and, especially, meropenem. this parenteral antibiotic may offer slightly more activity than meropenem against selected pathogens, including coverage of some strains of pseudomonas aeruginosa strains not susceptible to the other antipseudomonal carbapenems. empiric therapy with doripenem, as with other antipseudomona ... | 2006 | 16845443 |
| efflux pump genes of the resistance-nodulation-division family in burkholderia cenocepacia genome. | burkholderia cenocepacia is recognized as opportunistic pathogen that can cause lung infections in cystic fibrosis patients. a hallmark of b. cenocepacia infections is the inability to eradicate the organism because of multiple intrinsic antibiotic resistance. as resistance-nodulation-division (rnd) efflux systems are responsible for much of the intrinsic multidrug resistance in gram-negative bacteria, this study aims to identify rnd genes in the b. cenocepacia genome and start to investigate th ... | 2006 | 16857052 |
| a napkin-associated outbreak of burkholderia cenocepacia bacteraemia in haemodialysis patients. | this article reports a catheter-related outbreak of bacteraemia involving 38 patients in two haemodialysis units in verona. burkholderia cepacia complex strains were isolated from human blood and from an individually wrapped disinfection napkin that was contained in a commercially available, sterile dressing kit used to handle central venous catheters. micro-organisms isolated from blood cultures and from the napkin were identified by standard procedures and confirmed as b. cenocepacia (genomova ... | 2006 | 16859809 |
| exopolysaccharides produced by clinical strains belonging to the burkholderia cepacia complex. | in the frame of a research line dedicated to better clarify the role of exopolysaccharides (eps) in bacterial virulence, eps produced by species of the burkholderia cepacia complex (bcc), namely burkholderia multivorans, burkholderia cenocepacia, and a bcc member of undetermined genomovar, all isolated at the cystic fibrosis regional centre of florence (italy), were investigated for they structural properties. | 2007 | 16860003 |
| comparison by electron microscopy of intracellular events and survival of burkholderia pseudomallei in monocytes from normal subjects and patients with melioidosis. | burkholderia pseudomallei (b. pseudomallei) has been shown to persist intracellularly in patients with melioidosis, until reactivated by decreasing immunocompetence. we have previously demonstrated by transmission electron microscopy, the internalisation of b. pseudomallei by human macrophages and the occurrence of phagosome-lysosome fusion. | 2006 | 16865211 |
| substitutions of the "bridging" aspartate 178 result in profound changes in the reactivity of the rieske center of phthalate dioxygenase. | phthalate dioxygenase (pdo) and its reductase (pdr) are parts of a two-component rieske oxygenase system that initiates the aerobic breakdown of phthalate by forming cis-4,5-dihydro-4,5-dihydroxyphthalate. aspartate d178 in pdo, which lies between the rieske [2fe-2s] center of one subunit and the mononuclear center of the adjacent subunit, is highly conserved among the rieske dioxygenases. the analogous aspartate has been implicated in electron transfer in naphthalene dioxygenase and in substrat ... | 2006 | 16866348 |
| intracellular survival of burkholderia cenocepacia in macrophages is associated with a delay in the maturation of bacteria-containing vacuoles. | strains of the burkholderia cepacia complex (bcc) are opportunistic bacteria that can cause life-threatening infections in patients with cystic fibrosis and chronic granulomatous disease. previous work has shown that bcc isolates can persist in membrane-bound vacuoles within amoeba and macrophages without bacterial replication, but the detailed mechanism of bacterial persistence is unknown. in this study, we have investigated the survival of the burkholderia cenocepacia strain j2315 within raw26 ... | 2007 | 16869828 |
| functional genetic analysis reveals a 2-alkyl-4-quinolone signaling system in the human pathogen burkholderia pseudomallei and related bacteria. | pseudomonas aeruginosa synthesizes diverse 2-alkyl-4(1h)-quinolones (ahqs), including the signaling molecule 2-heptyl-3-hydroxy-4(1h)-quinolone (pqs), via the pqsabcde locus. by examining the genome databases, homologs of the pqs genes were identified in other bacteria. however, apart from p. aeruginosa, only burkholderia pseudomallei and b. thailandensis contained a complete pqsa-e operon (termed hhqa-e). by introducing the b. pseudomallei hhqa and hhqe genes into p. aeruginosa pqsa and pqse mu ... | 2006 | 16873018 |