| [quantitative detection of anti-classical swine fever virus sirna expression in cells by stem-loop rt-qpcr]. | rna interference (rnai) is a promising technology in development of specific antiviral therapy, but the quantitative detection of small interfering rna (sirna) expressed in vivo is the main challenge to assess its antiviral effect. in order to detect the sirna molecules (sin1 and sin2) particularly expressed in cells to inhibit the replication of classical swine fever virus (csfv), serial specific stem-loop primers were designed and synthesized. two of them (slp-n1-6 and slp-n2-8) were selected ... | 2012 | 22667106 |
| real-time analysis of the interaction of a multiple-epitope peptide with antibodies against classical swine fever virus using surface plasmon resonance. | the e2 envelope glycoprotein is the major immunodominant protein of classical swine fever virus (csfv), and can induce neutralizing antibodies and protective immune responses in infected swine. we developed a tandem-repeat multiple-epitope recombinant protein that contains two copies of each of the regions of e2 spanned by residues 693-704, 770-780, and 826-843, coupled by two copies of the region spanned by residues 1446-1460 of the csfv nonstructural protein ns2-3. the chemically synthesized g ... | 2012 | 22634031 |
| withdrawn: development of an rt-pcr-rflp assay for the detection and differentiation of wild-type and vaccine strains of classical swine fever virus. | this article has been withdrawn at the request of the author(s). the publisher apologizes for any inconvenience this may cause. the full elsevier policy on article withdrawal can be found at http://www.elsevier.com/locate/withdrawalpolicy. | 2012 | 22609180 |
| early pathogenesis of classical swine fever virus (csfv) strains in danish pigs. | host-virus interactions play an important role for the clinical outcome of classical swine fever virus (csfv) infections in pigs. strain virulence, host characteristics and environment are all factors that markedly influence disease severity. we tested csfv strains of varying virulence in an experimental set-up, reducing the influence of host and environmental factors. thus, weaner pigs were inoculated with one of 4 csfv strains in order to compare the pathogenesis for a 3-week-period after infe ... | 2012 | 22608103 |
| positive selection pressure on the b/c domains of the e2-gene of classical swine fever virus in endemic areas under c-strain vaccination. | in cuba, classical swine fever (csf) has become an endemic disease with several outbreaks each year, despite the implemented vaccination program. interestingly, a trend towards a milder presentation of the disease has been observed among the animals during the last years. this study aimed to assess positive selection pressure acting on partial e2 gene of csf viruses to gain insights into the mechanisms governing virulence and the driving forces of classical swine fever virus (csfv) evolution in ... | 2012 | 22580241 |
| development of multiplex pcr for simultaneous detection of six swine dna and rna viruses. | uniplex and multiplex reverse transcription-polymerase chain reaction (rt-pcr) and pcr protocols were developed and evaluated subsequently for its effectiveness in detecting simultaneously single and mixed infections in swine. specific primers for three dna viruses and three rna viruses, including classical swine fever virus (csfv), porcine reproductive and respiratory syndrome virus (prrsv), japanese encephalitis virus (jev), porcine circovirus type 2 (pcv2), porcine pseudorabies virus (prv) an ... | 2012 | 22575688 |
| prevalence of and risk factors associated with viral and bacterial pathogens in farmed european wild boar. | the aim of this study was to estimate in farmed european wild boars the prevalence of and risk factors associated with a range of common porcine viral and bacterial infections, namely, porcine parvovirus (ppv), porcine circovirus type 2 (pcv2), swine influenza virus (siv), aujeszky's disease virus (adv), classical swine fever virus (csfv), swine vesicular disease virus (svdv), coronavirus causing transmissible gastroenteritis (tgev), porcine reproductive and respiratory syndrome virus (prrsv), m ... | 2012 | 22516920 |
| characterization of c-strain "riems" tav-epitope escape variants obtained through selective antibody pressure in cell culture. | classical swine fever virus (csfv) c-strain "riems" escape variants generated under selective antibody pressure with monoclonal antibodies and a peptide-specific antiserum in cell culture were investigated. candidates with up to three amino acid exchanges in the immunodominant and highly conserved linear tav-epitope of the e2-glycoprotein, and additional mutations in the envelope proteins erns and e1, were characterized both in vitro and in vivo.it was further demonstrated, that intramuscular im ... | 2012 | 22515281 |
| baculovirus expression and antigenic characterization of classical swine fever virus e2 proteins. | genes encoding a major structural glycoprotein, e2, of classical swine fever viruses (csfv) brescia (subgroup 1.2), paderborn (subgroup 2.1) and kanagawa (subgroup 3.4) were constructed by removing the transmembrane domain and adding a c-terminal 6 histidine (his) tag. all the e2 constructs were efficiently expressed in a baculovirus system as 53-kda glycosylated proteins that were identified in western blots by their reaction with anti-his and csfv-specific antibodies. these proteins were used ... | 2013 | 22510427 |
| classical swine fever virus p7 protein is a viroporin involved in virulence in swine. | the nonstructural protein p7 of classical swine fever virus (csfv) is a small hydrophobic polypeptide with an apparent molecular mass of 6 to 7 kda. the protein contains two hydrophobic stretches of amino acids interrupted by a short charged segment that are predicted to form transmembrane helices and a cytosolic loop, respectively. using reverse genetics, partial in-frame deletions of p7 were deleterious for virus growth, demonstrating that csfv p7 function is critical for virus production in c ... | 2012 | 22496228 |
| a novel pcr-based method for high throughput prokaryotic expression of antimicrobial peptide genes. | to facilitate the screening of large quantities of new antimicrobial peptides (amps), we describe a cost-effective method for high throughput prokaryotic expression of amps. eddie, an autoproteolytic mutant of the n-terminal autoprotease, npro, from classical swine fever virus, was selected as a fusion protein partner. the expression system was used for high-level expression of six antimicrobial peptides with different sizes: bombinin-like peptide 7, temporin g, hexapeptide, combi-1, human hista ... | 2012 | 22439858 |
| establishment and characterization of an infectious cdna clone of a classical swine fever virus lom strain. | classical swine fever virus (csfv) causes a highly contagious disease among swine that has an important economic impact worldwide. csfv strain lom is an attenuated virus of low virulent strain of miyagi isolated from japan in 1956. eight dna fragments representing the genome of the csfv strain lom were obtained by rt-pcr. these were used to determine the complete nucleotide sequence and construct a full-length cdna clone which was called flc-lom. sequence analysis of the recombinant clone (flc-l ... | 2012 | 22437540 |
| characterisation of vaccine-induced, broadly cross-reactive ifn-γ secreting t cell responses that correlate with rapid protection against classical swine fever virus. | live attenuated c-strain classical swine fever viruses (csfv) provide a rapid onset of protection, but the lack of a serological test that can differentiate vaccinated from infected animals limits their application in csf outbreaks. since immunity may precede antibody responses, we examined the kinetics and specificity of peripheral blood t cell responses from pigs vaccinated with a c-strain vaccine and challenged after five days with a genotypically divergent csfv isolate. vaccinated animals di ... | 2012 | 22366027 |
| n(pro) of classical swine fever virus prevents type i interferon-mediated priming of conventional dendritic cells for enhanced interferon-α response. | a hallmark of acute classical swine fever is the high interferon (ifn)-α levels found in the serum early after infection, followed by an inflammatory cytokine storm. plasmacytoid dendritic cells (pdcs) represent the only known cell type that produces ifn-α upon classical swine fever virus (csfv) infection in vitro. in primary target cells of the virus the viral protein n(pro) inhibits the induction of type i ifn via the degradation of irf3. we hypothesized that the early systemic pdc-derived ifn ... | 2012 | 22313263 |
| yeast expressed classical swine fever e2 subunit vaccine candidate provides complete protection against lethal challenge infection and prevents horizontal virus transmission. | classical swine fever (csf) caused by the classical swine fever virus (csfv) is a highly contagious swine disease resulting in large economical losses worldwide. the viral envelope glycoprotein e(rns) and e2 are major targets for eliciting antibodies against csfv in infected animals. a pichia pastoris yeast expressed e2 protein (ye2) has been shown to induce a protective immune response against csfv challenge. the purpose of this study is to determine the optimal dose of ye2 and its efficacy on ... | 2012 | 22300723 |
| classical swine fever virus ns5a protein interacts with 3'-untranslated region and regulates viral rna synthesis. | to investigate the function of classical swine fever virus (csfv) ns5a protein, the experiments for viral rna synthesis and viral replication were performed in the co-presence of ns5a and ns5b. results showed that small concentrations of ns5a stimulated, large concentrations of ns5a inhibited, viral rna synthesis and viral replication. affinity chromatography experiments and uv-crosslinking assays revealed that csfv ns5a and ns5b bound its cognate 3'utr and that ns5a had higher affinity than ns5 ... | 2012 | 22261205 |
| effects of astragalus polysaccharide on immune responses of porcine pbmc stimulated with prrsv or csfv. | astragalus polysaccharide (aps) has been used as an immunomodulator that can enhance immune responses, whereas the immunomodulatory effects of aps on porcine peripheral blood mononuclear cells (pbmcs) exposed to porcine reproductive and respiratory syndrome virus (prrsv) and classical swine fever virus (csfv) have not been investigated. | 2012 | 22253710 |
| molecular characterization of classical swine fever virus involved in the outbreak in mizoram. | classical swine fever is the most insidious and devastating disease of pigs and wild boars. the virus is closely related to the other members of the genus pestivirus. the outbreak recorded in mizoram, india was strategically important as the state shares porous international boundary with east asian countries. both immunodiagnostic and molecular techniques were used to confirm the involvement of classical swine fever virus (csfv) in this outbreak. sandwich elisa and direct fat could detect csfv ... | 2010 | 23637482 |
| characterization and expression of e2 glycoprotein of classical swine fever virus in a eukaryotic expression system. | classical swine fever (csf) is an economically important office international des epizooties (oie) list a disease of swine characterized by high fever and multiple haemmorhages. the e2 glycoprotein of csfv is immunogenic and induces neutralizing antibodies against csfv. in the present study, complete coding region of the e2 gene from indian virulent field isolate (mathura) was amplified by reverse transcription-polymerase chain reaction (rt-pcr) and subsequently cloned into a mammalian expressio ... | 2010 | 23637481 |
| classical swine fever virus diagnostics and vaccine production in insect cells. | | 1996 | 22358490 |
| neurological disorders, virus persistence and hypomyelination in calves due to intra-uterine infections with bovine virus diarrhoea virus. | summary the clinical and pathological findings after a natural intra-uterine infection with bvd-virus in a friesian dairy herd are described. the virological and serological aspects will be discussed in a separate paper (30). in a period of 4 years, 11 calves were hum with the following nervous symptoms: more or less serious incoördination, tremor, oscillating nystagmus, and a negative blinking reflex. the pupillary and sucking reflexe's were normal. no ocular defects, such as lenticular opacity ... | 1983 | 22040145 |
| pacmanvirus, a new giant icosahedral virus at the crossroads between asfarviridae and faustoviruses. | african swine fever virus, a double-stranded dna virus that infects pigs, is the only known member of the asfarviridae family. nevertheless, during our isolation and sequencing of the complete genome of faustovirus, followed by the description of kaumoebavirus, carried out over the past 2 years, we observed the emergence of previously unknown related viruses within this group of viruses. here we describe the isolation of pacmanvirus, a fourth member in this group, which is capable of infecting a ... | 2017 | 28446673 |
| novel pestivirus species in pigs, austria, 2015. | a novel pestivirus species was discovered in a piglet-producing farm in austria during virologic examinations of congenital tremor cases. the emergence of this novel pestivirus species, provisionally termed linda virus, in domestic pigs may have implications for classical swine fever virus surveillance and porcine health management. | 2017 | 28628456 |
| seroprevalence of economically important viral pathogens in swine populations of trinidad and tobago, west indies. | the objective of this study was to evaluate the seroprevalence and identify the strains of swine influenza virus (swiv), as well as the seroprevalence of porcine parvovirus (ppv), transmissible gastroenteritis virus (tgev), porcine reproductive and respiratory syndrome virus (prrsv), porcine respiratory coronavirus (prcv), porcine circovirus type 2 (pcv-2), and classical swine fever virus (csfv) in pigs in trinidad and tobago (t&t). blood samples (309) were randomly collected from pigs at farms ... | 2017 | 28523387 |
| investigations of pro- and anti-apoptotic factors affecting african swine fever virus replication and pathogenesis. | african swine fever virus (asfv) is a large dna virus that replicates predominantly in the cell cytoplasm and is the only member of the asfarviridae family. the virus causes an acute haemorrhagic fever, african swine fever (asf), in domestic pigs and wild boar resulting in the death of most infected animals. apoptosis is induced at an early stage during virus entry or uncoating. however, asfv encodes anti-apoptotic proteins which facilitate production of progeny virions. these anti-apoptotic pro ... | 2017 | 28841179 |
| ba71δcd2: a new recombinant live attenuated african swine fever virus with cross-protective capabilities. | african swine fever is a highly contagious viral disease of mandatory declaration to the world organization for animal health (oie). lack of available vaccines make its control difficult and thus asfv represents a major threat to the swine industry. inactivated vaccines do not confer solid protection against african swine fever virus (asfv). conversely, live attenuated viruses (lav), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable protection against homo ... | 2017 | 28814514 |
| estimation of the transmission dynamics of african swine fever virus within a swine house. | the spread of african swine fever virus (asfv) threatens to reach further parts of europe. in countries with a large swine production, an outbreak of asf may result in devastating economic consequences for the swine industry. simulation models can assist decision makers setting up contingency plans. this creates a need for estimation of parameters. this study presents a new analysis of a previously published study. a full likelihood framework is presented including the impact of model assumption ... | 2017 | 28768556 |
| a proteomic insight into the midgut proteome of ornithodoros moubata females reveals novel information on blood digestion in argasid ticks. | the argasid tick ornithodoros moubata is the main african vector of the human relapsing fever agent borrelia duttoni and the african swine fever virus. together with saliva, the tick midgut forms part of the host-tick-pathogen interface, and numerous midgut proteins play key functions in the blood digestion-related process and the infection and transmission of pathogens. this work explores the composition of the midgut proteome of unfed and fed o. moubata females with the aim to complete the bio ... | 2017 | 28764815 |
| african swine fever virus infection in classical swine fever subclinically infected wild boars. | recently moderate-virulence classical swine fever virus (csfv) strains have been proven capable of generating postnatal persistent infection (pi), defined by the maintenance of viremia and the inability to generate csfv-specific immune responses in animals. these animals also showed a type i interferon blockade in the absence of clinical signs. in this study, we assessed the infection generated in 7-week-old csfv pi wild boars after infection with the african swine fever virus (asfv). the wild b ... | 2017 | 28764692 |
| evaluation of blowfly larvae (diptera: calliphoridae) as possible reservoirs and mechanical vectors of african swine fever virus. | in 2014, highly virulent african swine fever virus (asfv) was introduced into the baltic states and poland, with new cases being reported almost every week from wild boar and also from domestic pigs. contrary to initial predictions that the disease would either die out due to the high virulence of the virus strain or spread rapidly in westerly direction, the infection became endemic and spread slowly. the unexpected disease epidemiology led to the hypothesis that hitherto unconsidered factors mi ... | 2017 | 28762629 |
| vaccinia virus and cowpox virus are not susceptible to the interferon-induced antiviral protein mxa. | mxa protein is expressed in response to type i and type iii interferon and constitute an important antiviral factor with broad antiviral activity to diverse rna viruses. in addition, some studies expand the range of mxa antiviral activity to include particular dna viruses like monkeypox virus (mpxv) and african swine fever virus (asfv). however, a broad profile of activity of mxa to large dna viruses has not been established to date. here, we investigated if some well characterized dna viruses b ... | 2017 | 28727764 |
| detection of african swine fever virus in the tissues of asymptomatic pigs in smallholder farming systems along the kenya-uganda border: implications for transmission in endemic areas and asf surveillance in east africa. | the persistence of african swine fever virus (asfv) in endemic areas, with small-scale but regular outbreaks in domestic pigs, is not well understood. asfv has not been detected using conventional diagnosis in these pigs or adjacent populations of resistant african wild pigs, that could act as potential carriers during the outbreaks. however, such data are crucial for the design of evidence-based control strategies. we conducted cross-sectional (1107 pigs) and longitudinal (100 pigs) monitoring ... | 2017 | 28721858 |
| virulent strain of african swine fever virus eclipses its attenuated derivative after challenge. | african swine fever (asf) is one of the most devastating diseases affecting the swine industry worldwide. no effective vaccine is currently available for disease prevention and control. although live attenuated vaccines (lav) have demonstrated great potential for immunizing against homologous strains of african swine fever virus (asfv), adverse reactions from lav remain a concern. here, by using a homologous asfv congo strain system, we show passage-attenuated congo lav to induce an efficient pr ... | 2017 | 28691128 |
| redistribution of endosomal membranes to the african swine fever virus replication site. | african swine fever virus (asfv) infection causes endosomal reorganization. here, we show that the virus causes endosomal congregation close to the nucleus as the infection progresses, which is necessary to build a compact viral replication organelle. asfv enters the cell by the endosomal pathway and reaches multivesicular late endosomes. upon uncoating and fusion, the virus should exit to the cytosol to start replication. asfv remodels endosomal traffic and redistributes endosomal membranes to ... | 2017 | 28587154 |
| selection of differently temporally regulated african swine fever virus promoters with variable expression activities and their application for transient and recombinant virus mediated gene expression. | african swine fever virus threatens pig production worldwide due to the lack of vaccines, for which generation of both deletion and insertion mutants is considered. for development of the latter, operational asfv promoters of different temporal regulation and strengths are desirable. we therefore compared the capacities of putative promoter sequences from p72, cd2v, p30, viral dna polymerase and u104l genes to mediate expression of luciferase from transfected plasmids after activation in trans, ... | 2017 | 28502836 |
| comparative phenotypic and functional analyses of the effects of autologous plasma and recombinant human macrophage-colony stimulating factor (m-csf) on porcine monocyte to macrophage differentiation. | porcine monocyte-derived macrophages (momφ) have been employed as a model cell in numerous studies of the porcine immune system. however, the lack of a standardized method for momφ differentiation hampers the comparison of results coming from the use of different laboratory protocols. in this study we compared the use of varying concentrations of autologous plasma (10, 20 and 30% v/v) or recombinant human macrophage-colony stimulating factor (hm-csf; 50, 100, and 200ng/ml) to differentiate porci ... | 2017 | 28494933 |
| hemophagocytic lymphohistiocytosis in acute african swine fever clinic. | hemophagocytic lymphohistiocytosis (hlh) usually has been defined as the combination of a proliferation of cytologically benign, actively phagocytic macrophages in bone marrow, spleen, lymph nodes, etc. in association with fever, cytopenia, splenomegaly, and hypertriglyceridemia. hlh is often triggered by viral infection. the aim of this study was to ascertain the features of hlh involvement in african swine fever virus (asfv) (genotype ii) pathogenesis. | 2017 | 28494931 |
| african swine fever virus: a review. | african swine fever (asf) is a highly contagious viral disease of swine which causes high mortality, approaching 100%, in domestic pigs. asf is caused by a large, double stranded dna virus, asf virus (asfv), which replicates predominantly in the cytoplasm of macrophages and is the only member of the asfarviridae family, genus asfivirus. the natural hosts of this virus include wild suids and arthropod vectors of the ornithodoros genus. the infection of asfv in its reservoir hosts is usually asymp ... | 2017 | 28489063 |
| adenovirus-vectored novel african swine fever virus antigens elicit robust immune responses in swine. | african swine fever virus (asfv) is a high-consequence transboundary animal pathogen that often causes hemorrhagic disease in swine with a case fatality rate close to 100%. lack of treatment or vaccine for the disease makes it imperative that safe and efficacious vaccines are developed to safeguard the swine industry. in this study, we evaluated the immunogenicity of seven adenovirus-vectored novel asfv antigens, namely a151r, b119l, b602l, ep402rδprr, b438l, k205r and a104r. immunization of com ... | 2017 | 28481911 |
| pig traders' networks on the kenya-uganda border highlight potential for mitigation of african swine fever virus transmission and improved asf disease risk management. | we applied social network analysis to pig trader networks on the kenya-uganda border. social network analysis is a recently developed tool, which is useful for understanding value chains and improving disease control policies. we interviewed a sample of 33 traders about their experiences with trade and african swine fever (asf), analyzed the networks they generated in purchasing pigs and selling pork and their potential contribution to modulating dissemination of the asf virus (asfv). the majori ... | 2017 | 28460754 |
| development of a fluorescent asfv strain that retains the ability to cause disease in swine. | african swine fever is a contagious and often lethal disease for domestic pigs with a significant economic impact for the swine industry. the etiological agent, african swine fever virus (asfv), is a highly structurally complex double stranded dna virus. no effective vaccines or antiviral treatment are currently commercially available. we present here the development of a strain of asfv that has been shown to retain its ability to cause disease in swine, efficiently replicate in swine macrophage ... | 2017 | 28436458 |
| proposed revision to the taxonomy of the genus pestivirus, family flaviviridae. | we propose the creation of seven new species in the genus pestivirus (family flaviviridae) in addition to the four existing species, and naming species in a host-independent manner using the format pestivirus x. only the virus species names would change; virus isolates would still be referred to by their original names. the original species would be re-designated as pestivirus a (original designation bovine viral diarrhea virus 1), pestivirus b (bovine viral diarrhea virus 2), pestivirus c (clas ... | 2017 | 28786787 |
| detection of border disease virus in mexican cattle. | the genus pestivirus within flaviviridae is comprised of four recognized species, namely, bovine viral diarrhoea virus 1 (bvdv-1), bovine viral diarrhoea virus 2 (bvdv-2), border disease virus (bdv) and classical swine fever virus (csfv). bdv, while primarily infecting sheep and goats, has also been reported in cattle and wild animals. infections of sheep and goats result in economic loss due to abortions and the birth of persistently infected animals that have poor production and reduced life e ... | 2017 | 28474402 |
| a novel viewrna in situ hybridization method for the detection of the dynamic distribution of classical swine fever virus rna in pk15 cells. | classical swine fever (csf) is a highly contagious fatal infectious disease caused by classical swine fever virus (csfv). a better understanding of csfv replication is important for the study of pathogenic mechanism of csf. with the development of novel rna in situ hybridization method, quantitatively localization and visualization of the virus rna molecular in cultured cell or tissue section becomes very important tool to address these pivotal pathogenic questions. in this study, we established ... | 2017 | 28420390 |
| pre-screening of crude peptides in a serological bead-based suspension array. | most serological assays detect antibody responses in biological samples through affinity of serum antibodies for antigens provided in the assay. certain antigens, however, may be difficult to produce and/or may contain unwanted epitopes. in these cases, a practical alternative may be the use of peptides as representatives for specific epitopes. peptides can be obtained after purification in large quantities for a modest price, but screening of a large set of peptides during development may be re ... | 2017 | 28545817 |
| [expression and antiviral activity of a chimeric porcinized monoclonal antibody (chq06) against e2 protein of classical swine fever virus]. | classical swine fever (csf), one of oie-listed diseases, is a highly contagious and economically important disease of pigs. classical swine fever virus (csfv) is the causative agent of csf. the capsid (c) protein and the glycoproteins erns, e1 and e2, are structural components of the virus. e2 is the most immunogenic protein of the csfv glycoproteins, inducing neutralizing antibodies that provide protection against lethal csfv challenge. in a previous study, we developed a murine mab hq06 agains ... | 2017 | 28853251 |
| rab5 enhances classical swine fever virus proliferation and interacts with viral ns4b protein to facilitate formation of ns4b related complex. | classical swine fever virus (csfv) is a fatal pig pestivirus and causes serious financial losses to the pig industry. csfv ns4b protein is one of the most important viral replicase proteins. rab5, a member of the small rab gtpase family, is involved in infection and replication of numerous viruses including hepatitis c virus and dengue virus. until now, the effects of rab5 on the proliferation of csfv are poorly defined. in the present study, we showed that rab5 could enhance csfv proliferation ... | 2017 | 28848503 |
| the uncoupling of protease trans-cleavage and helicase activities in the pestivirus ns3. | the nonstructural protein ns3 from the flaviviridae family is a multi-functional protein that contains an n-terminal protease and a c-terminal helicase, playing essential roles in viral polyprotein processing and genome replication. here we report a full-length crystal structure of the classical swine fever virus (csfv) ns3 in complex with its ns4a protease cofactor segment (pcs) at 2.35 å resolution. the structure reveals a previously unidentified ∼2200-å(2) intra-molecular protease-helicase in ... | 2017 | 28835495 |
| epidemiology, diagnosis and control of classical swine fever: recent developments and future challenges. | classical swine fever (csf) represents a major health and trade problem for the pig industry. in endemic countries or those with a wild boar reservoir, csf remains a priority for veterinary services. surveillance as well as stamping out and/or vaccination are the principle tools of prevention and control, depending on the context. in the past decades, marker vaccines and accompanying diagnostic tests allowing the discrimination of infected from vaccinated animals have been developed. in the euro ... | 2017 | 28795533 |
| changing pattern of classical swine fever virus genogroup from classical 1.1 to emerging 2.2 in india. | classical swine fever (csf) is one of the most important viral diseases of pigs with high economic impact. the causative agent, classical swine fever virus (csfv) is a member of genus pestivirus in family flaviviredae and is structurally and antigenically related to other members of the genus. the identification of virus strains and genotypes can conveniently be used to trace the origin and patterns of virus spread, which contribut substantially in control strategies. in the present study, we ha ... | 2017 | 28770243 |
| traf6 is a novel ns3-interacting protein that inhibits classical swine fever virus replication. | classical swine fever virus (csfv) non-structural protein 3 (ns3) is a multifunctional non-structural protein that plays a major role in viral replication. however, how exactly ns3 exerts these functions remains unknown. here, we identified tumour necrosis factor receptor-associated factor 6 (traf6) as a novel ns3-interacting protein via yeast two-hybrid analysis, co-immunoprecipitation, and glutathione s-transferase pull-down assays. furthermore, we observed that traf6 overexpression significan ... | 2017 | 28751780 |
| identification of the linear ligand epitope on classical swine fever virus that interacts with porcine kidney 15 cells. | binding of the viral ligand to a specific receptor is the first step of virus entry into target cells. the envelope proteins e(rns), e1, and e2 of classical swine fever virus (csfv) are involved in the interaction with host cell receptors to mediate csfv infection. the aim of this investigation was to identify epitopes that bind to porcine kidney (pk)-15 cells to prevent csfv infection. ten peptides representing e(rns), e1, and e2 were synthesized. immunohistochemical study showed that the se24 ... | 2017 | 28725108 |
| us10, a novel npro-interacting protein, inhibits classical swine fever virus replication. | classical swine fever (csf) is a severe, febrile and highly contagious disease caused by classical swine fever virus (csfv) that has resulted in huge economic losses in the pig industry worldwide. csfv npro has been actively studied but remains incompletely understood. few studies have investigated the cellular proteins that interact with npro and their participation in viral replication. here, the yeast two-hybrid (y2h) system was employed to screen npro-interacting proteins from a porcine alve ... | 2017 | 28721853 |
| classical swine fever virus in cattle. | | 2017 | 28710174 |
| continuous passaging of a recombinant c-strain virus in pk-15 cells selects culture-adapted variants that showed enhanced replication but failed to induce fever in rabbits. | classical swine fever virus (csfv) is the etiologic agent of classical swine fever (csf), a highly contagious disease that causes significant economic losses to the swine industry. the lapinized c-strain, a widely used vaccine strain against csfv, has low growth efficiency in cell culture, thus limits the productivity in vaccine industry. in this study, a recombinant virus derived from c-strain was constructed and subjected to continuous passaging in pk-15 cells with the goal of acquiring high p ... | 2017 | 28704902 |
| a single dose of the novel chimeric subunit vaccine e2-cd154 confers early full protection against classical swine fever virus. | classical swine fever is an economically important, highly contagious disease of swine worldwide. subunit vaccines are a suitable alternative for the control of classical swine fever. however, such vaccines have as the main drawback the relatively long period of time required to induce a protective response, which hampers their use under outbreak conditions. in this work, a lentivirus-based gene delivery system is used to obtain a stable recombinant hek 293 cell line for the expression of e2-csf ... | 2017 | 28688785 |
| an immuno-chromatographic lateral flow assay (lfa) for rapid on-the-farm detection of classical swine fever virus (csfv). | classical swine fever (csf) is a highly contagious and potentially fatal disease of domestic pigs. classical swine fever is routinely diagnosed by clinical signs, serology, detection of csf virus (csfv) nucleic acid by pcr and virus isolation. most of the current csf diagnostic methods are expensive and have an extended turnaround time. in the majority of the csf endemic countries, lack of easy access to diagnostic facilities is a major problem for swine producers trying to obtain early diagnosi ... | 2017 | 28685287 |
| complex virus-host interactions involved in the regulation of classical swine fever virus replication: a minireview. | classical swine fever (csf), caused by classical swine fever virus (csfv), is one of the most devastating epizootic diseases of pigs in many countries. viruses are small intracellular parasites and thus rely on the cellular factors for replication. fundamental aspects of csfv-host interactions have been well described, such as factors contributing to viral attachment, modulation of genomic replication and translation, antagonism of innate immunity, and inhibition of cell apoptosis. however, thos ... | 2017 | 28678154 |
| efficacy of e2 glycoprotein fused to porcine cd154 as a novel chimeric subunit vaccine to prevent classical swine fever virus vertical transmission in pregnant sows. | here we evaluated the effect of double vaccination with a novel subunit marker vaccine candidate based in the csfv e2 glycoprotein fused to the porcine cd154 to prevent csfv vertical transmission. a lentivirus-based gene delivery system was used to obtain a stable recombinant hek 293 cell line for the expression of e2 fused to porcine cd154 molecule. six pregnant sows were distributed in two groups and at 64days of gestation animals numbered 1-4 (group 1) were vaccinated via intramuscular inocul ... | 2017 | 28622852 |
| recombinant swinepox virus expressing glycoprotein e2 of classical swine fever virus confers complete protection in pigs upon viral challenge. | classical swine fever (csf) is a highly contagious and serious viral disease that affects the pig industry worldwide. the glycoprotein e2 of the classical swine fever virus (csfv) can induce neutralizing antibodies, and it is widely used for novel vaccine development. to explore the development of a vaccine against csfv infections, the gene of glycoprotein e2 was inserted into the swinepox virus (spv) genome by homologous recombination. the culture titers of rspv-e2 remained at about 4.3 × 10(6) ... | 2017 | 28612010 |
| novel thioglycosyl analogs of glycosyltransferase substrates as antiviral compounds against classical swine fever virus and hepatitis c virus. | hepatitis c virus (hcv) and classical swine fever virus (csfv) are important pathogens for which new therapeutic approaches are in high demand. herein, we report the synthesis of newly designed thioglycosyl analogs of glycosyltransferase substrates which were evaluated using cell-based assays for cytotoxicity and antiviral activity against both viruses. the antiviral activity of synthesized compounds against csfv and hcv was confirmed using pseudo-plaque reduction assays where a significant arre ... | 2017 | 28601004 |
| synergistic roles of the e2 glycoprotein and 3' untranslated region in the increased genomic stability of chimeric classical swine fever virus with attenuated phenotypes. | the e2 glycoprotein and 3' untranslated region (utr) of classical swine fever virus (csfv) are virulence determinants. to investigate the synergistic roles of e2 and 3'utr for pathogenicity and genomic stability, a series of chimeric csfvs were constructed by replacing the e2 gene and/or 3'utr of virulent csfv strain shimen with the corresponding sequence of the lapinized 'chinese' strain (c-strain) using a reverse genetic approach. the in vitro growth characterization and in vivo pathogenicity ... | 2017 | 28573473 |
| a bivalent dendrimeric peptide bearing a t-cell epitope from foot-and-mouth disease virus protein 3a improves humoral response against classical swine fever virus. | three dendrimeric peptides were synthesized in order to evaluate their immunogenicity and their potential protection against classical swine fever virus (csfv) in domestic pigs. construct 1, an optimized version of a previously used dendrimer, had four copies of a b-cell epitope derived from csfv e2 glycoprotein connected to an also csfv-derived t-cell epitope through maleimide instead of thioether linkages. construct 2 was similarly built but included only two copies of the b-cell epitope, and ... | 2017 | 28571760 |
| effects of vaccination with the c-strain vaccine on immune cells and cytokines of pigs against classical swine fever virus. | the attenuated c-strain vaccine against classical swine fever virus (csfv) is one of the safest and most effective attenuated vaccines. however, little is known of the host immune response after vaccination with the c-strain vaccine. blood samples from vaccinated pigs were collected to evaluate the number of immune cells, the level of specific csfv antibody, and related cytokines induced by the vaccination of c-strain vaccine. the c-strain nucleic acid was gradually removed and specific antibody ... | 2017 | 28514189 |
| surface displaying of swine igg1 fc enhances baculovirus-vectored vaccine efficacy by facilitating viral complement escape and mammalian cell transduction. | baculovirus-mediated gene transfer has been developed as a vaccine design strategy against a number of diseases without apparent viral replication. however, it has been hampered by complement-dependent inactivation, thus hindering the in vivo application of baculovirus. a variety of approaches have been exploited to bypass the complement system in the serum. in this study, we constructed and screened a series of baculovirus vectors displaying complement interfering factors, of which a baculoviru ... | 2017 | 28499403 |
| serum metabolomic profiling of piglets infected with virulent classical swine fever virus. | classical swine fever (csf) is a highly contagious swine infectious disease and causes significant economic losses for the pig industry worldwide. the objective of this study was to determine whether small molecule metabolites contribute to the pathogenesis of csf. birefly, serum metabolomics of csfv shimen strain-infected piglets were analyzed by ultraperformance liquid chromatography/electrospray ionization time-of-flight mass spectrometry (uplc/esi-q-tof/ms) in combination with multivariate s ... | 2017 | 28496435 |
| metabolic profiles in cell lines infected with classical swine fever virus. | viruses require energy and biosynthetic precursors from host cells for replication. an understanding of the metabolic interplay between classical swine fever virus (csfv) and host cells is important for exploring the complex pathological mechanisms of classical swine fever (csf). in the current study, and for the first time, we utilized an approach involving gas chromatography coupled with mass spectrometry (gc-ms) to examine the metabolic profiles within pk-15 and 3d4/2 cells infected with csfv ... | 2017 | 28473819 |
| phylogenetic analysis of classical swine fever virus from archival formalin fixed clinical tissues reveals vietnamese origin of the isolates. | detection of classical swine fever virus (csfv) nucleic acid in archival formalin fixed tissue samples and their use for phylogenetic analysis was investigated. ten samples were examined for the presence of csfv nucleic acid by reverse transcriptase polymerase chain reaction (rt-pcr) amplification of 5'utr and e2 gene. rt-pcr was found positive for 5'utr fragment in eight samples while only one tissue samples showed amplification for e2 gene target fragment. for molecular epidemiology of the dis ... | 2017 | 28466064 |
| csfv induced mitochondrial fission and mitophagy to inhibit apoptosis. | classical swine fever virus (csfv), which causes typical clinical characteristics in piglets, including hemorrhagic syndrome and immunosuppression, is linked to hepatitis c and dengue virus. oxidative stress and a reduced mitochondrial transmembrane potential are disturbed in csfv-infected cells. the balance of mitochondrial dynamics is essential for cellular homeostasis. in this study, we offer the first evidence that csfv induces mitochondrial fission and mitophagy to inhibit host cell apoptos ... | 2017 | 28455958 |
| development of isothermal recombinase polymerase amplification assay for rapid detection of porcine circovirus type 2. | porcine circovirus virus type ii (pcv2) is the etiology of postweaning multisystemic wasting syndrome (pmws), porcine dermatitis, nephropathy syndrome (pdns), and necrotizing pneumonia. rapid diagnosis tool for detection of pcv2 plays an important role in the disease control and eradication program. recombinase polymerase amplification (rpa) assays using a real-time fluorescent detection (pcv2 real-time rpa assay) and rpa combined with lateral flow dipstick (pcv2 rpa lfd assay) were developed ta ... | 2017 | 28424790 |
| comparative characterization analysis of synonymous codon usage bias in classical swine fever virus. | classical swine fever virus (csfv) is responsible for the highly contagious viral disease of swine, and causes great economic loss in the swine-raising industry. considering the significance of csfv, a systemic analysis was performed to study its codon usage patterns. in this study, using the complete genome sequences of 76 csfv representing three genotypes, we firstly analyzed the relative nucleotide composition, effective number of codon (enc) and synonymous codon usage in csfv genomes. the re ... | 2017 | 28416383 |
| genetic and virulence characterization of classical swine fever viruses isolated in mongolia from 2007 to 2015. | classical swine fever (csf), a highly contagious viral disease affecting domestic and wild pigs in many developing countries, is now considered endemic in mongolia, with 14 recent outbreaks in 2007, 2008, 2011, 2012, 2014, and 2015. for the first time, csf viruses isolated from these 14 outbreaks were analyzed to assess their molecular epidemiology and pathogenicity in pigs. based on the nucleotide sequences of their 5'-untranslated region, isolates were phylogenetically classified as either sub ... | 2017 | 28260187 |
| predominance of genotype 1.1 and emergence of genotype 2.2 classical swine fever viruses in north-eastern region of india. | classical swine fever (csf) is a highly contagious and the most important disease of pigs worldwide.csf is enzootic in pig herds in india and continues to cause huge economic losses to pig farmers. nearly 40% of the total pig population of india is present in the north-eastern (ne) states where pig husbandry plays an important role in the socio-economic development. pigs reared in the backyards are the only source of livelihood for a majority of poor tribal population in the region. hardly any c ... | 2014 | 25135466 |
| susceptibility of cultred mammalian cells to infection with virulent and modified hog cholera viruses. | | 1968 | 4967124 |
| new leaves in the growing tree of pestiviruses. | pestiviruses are a group of viruses of veterinary importance infecting livestock animals like pigs, cattle, and sheep, and also wildlife animals like wild boar and different deer species. while for decades only four classical species (classical swine fever virus, bovine viral diarrhea virus types 1 and 2, border disease virus), and a few so-called atypical pestiviruses were known (e.g., giraffe virus, pronghorn virus, hobi virus), a series of novel pestiviruses was identified in the last years ( ... | 2017 | 29029724 |
| genetic characterization of african swine fever virus isolates from soft ticks at the wildlife/domestic interface in mozambique and identification of a novel genotype. | african swine fever virus (asfv) is one of the most threatening infectious diseases of pigs. there are not sufficient data to indicate the importance of the sylvatic cycle in the spread and maintenance of the disease locally and potentially, globally. to assess the capacity to maintain asf in the environment, we investigated the presence of soft tickreservoirs of asfv in gorongosa national park (gnp) and its surrounding villages. a total of 1,658 soft ticks were recovered from warthog burrows an ... | 2017 | 28921895 |
| efficient inhibition of african swine fever virus replication by crispr/cas9 targeting of the viral p30 gene (cp204l). | african swine fever is a devastating viral disease of domestic and wild pigs against which no vaccine or therapy is available. therefore, we applied the crispr (clustered regularly interspaced short palindromic repeats) - cas9 nuclease system to target the double-stranded dna genome of african swine fever virus (asfv). to this end, a permissive wild boar lung (wsl) cell line was modified by stable transfection with a plasmid encoding cas9 and a guide rna targeting codons 71 to 78 of the phosphop ... | 2018 | 29362418 |
| ictv virus taxonomy profile: asfarviridae. | the family asfarviridae includes the single species african swine fever virus, isolates of which have linear dsdna genomes of 170-194 kbp. virions have an internal core, an internal lipid membrane, an icosahedral capsid and an outer lipid envelope. infection of domestic pigs and wild boar results in an acute haemorrhagic fever with transmission by contact or ingestion, or by ticks of the genus ornithodoros. indigenous pigs act as reservoirs in africa, where infection is endemic, and from where i ... | 2018 | 29565243 |
| african swine fever virus biology and vaccine approaches. | african swine fever (asf) is an acute and often fatal disease affecting domestic pigs and wild boar, with severe economic consequences for affected countries. asf is endemic in sub-saharan africa and the island of sardinia, italy. since 2007, the virus emerged in the republic of georgia, and since then spread throughout the caucasus region and russia. outbreaks have also been reported in belarus, ukraine, lithuania, latvia, estonia, romania, moldova, czech republic, and poland, threatening neigh ... | 2018 | 29551143 |
| frequent infection of wild boar with atypical porcine pestivirus (appv). | the recently identified atypical porcine pestivirus (appv) was demonstrated to be the causative agent of the neurological disorder "congenital tremor" in newborn piglets. despite its relevance and wide distribution in domestic pigs, so far nothing is known about the situation in wild boar, representing an important wild animal reservoir for the related classical swine fever virus. in this study, 456 wild boar serum samples obtained from northern germany were investigated for the presence of appv ... | 2018 | 29527814 |
| modulation of type i interferon signaling by african swine fever virus (asfv) of different virulence l60 and nhv in macrophage host cells. | asfv causes an important disease of domestic swine and wild boar. currently no vaccine is available, highlighting the necessity to understand asfv modulation of innate immune responses in natural host cells. with this aim, macrophage cultures enriched in swc9 and cd163 differentiation markers were infected in parallel with high virulent asfv/l60 and low virulent asfv/nhv, the latter lacking mgf 360 and 505/530 genes associated with type i interferon (ifn i) control. ifn i production and signalin ... | 2018 | 29519508 |
| a review of african swine fever and the potential for introduction into the united states and the possibility of subsequent establishment in feral swine and native ticks. | african swine fever (asf) is caused by african swine fever virus (asfv), which can cause substantial morbidity and mortality events in swine. the virus can be transmittedviadirect and indirect contacts with infected swine, their products, or competent vector species, especiallyornithodorosticks. africa and much of eastern europe are endemic for asf; a viral introduction to countries that are currently asf free could have severe economic consequences due to the loss of production from infected an ... | 2018 | 29468165 |
| short time window for transmissibility of african swine fever virus from a contaminated environment. | since the introduction of african swine fever virus (asfv) into the baltic states and poland in 2014, the disease has continued to spread within these regions. in 2017, the virus spread further west and the first cases of disease were reported in the czech republic and romania, in wild boar and domestic pigs, respectively. to control further spread, knowledge of different modes of transmission, including indirect transmission via a contaminated environment, is crucial. up until now, such an indi ... | 2018 | 29457702 |
| a wide-ranging antiviral response in wild boar cells is triggered by non-coding synthetic rnas from the foot-and-mouth disease virus genome. | foot-and-mouth disease virus (fmdv) is the causative agent of a highly contagious viral disease that affects multiple cloven-hooved hosts including important livestock (pigs, cattle, sheep and goats) as well as several wild animal species. crossover of fmdv between domestic and wildlife populations may prolong virus circulation during outbreaks. the wild boar (sus scrofa) is considered a reservoir of various pathogens that can infect other wildlife, domestic animals, and humans. as wild boar and ... | 2020 | 32851049 |
| health profile of free-range wild boar (sus scrofa) subpopulations hunted in santa catarina state, brazil. | wild boars represent a potential dissemination source of important pathogens to public and animal health, since they can host several pathogens. this study aimed to evaluate the health profile of wild boars hunted for population control in the state of santa catarina (sc), south of brazil. for this study, tissue and blood samples were collected from 61 wild boars hunted from october 2017 to november 2018. all 61 serum samples were screened for antibodies by different assays, presenting the follo ... | 2020 | 32725949 |
| african swine fever virus mgf360-12l inhibits type i interferon production by blocking the interaction of importin α and nf-κb signaling pathway. | african swine fever (asf) is an infectious transboundary disease of domestic pigs and wild boar and spreading throughout eurasia. there is no vaccine and treatment available. complex immune escape strategies of african swine fever virus (asfv) are crucial factors affecting immune prevention and vaccine development. mgf360 genes have been implicated in the modulation of the ifn-i response. the molecular mechanisms contributing to innate immunity are poorly understood. in this study, we demonstrat ... | 2020 | 33141406 |
| whole-genome sequence of an african swine fever virus isolate from the czech republic. | between june 2017 and april 2018, an outbreak of african swine fever (asf) affected wild boar in the southeast of the czech republic. here, we present the whole-genome sequence of the causative asf virus. it belongs to genotype ii and shows very high identity with other strains from eastern europe. | 2020 | 33122412 |
| identification and isolation of two different subpopulations within african swine fever virus arm/07 stock. | no efficient vaccines exist against african swine fever virus (asfv), which causes a serious disease in wild boars and domestic pigs that produces great industrial and ecological concerns worldwide. an extensive genetic characterization of the original asfv stocks used to produce live attenuated vaccine (lav) prototypes is needed for vaccine biosecurity and control. here, we sequenced for the first time the arm/07 stock which was obtained from an infected pig during the armenia outbreak in 2007, ... | 2020 | 33113838 |
| fecal microbiota transplantation from warthog to pig confirms the influence of the gut microbiota on african swine fever susceptibility. | african swine fever virus (asfv) is the causative agent of a devastating hemorrhagic disease (asf) that affects both domestic pigs and wild boars. conversely, asfv circulates in a subclinical manner in african wild pigs, including warthogs, the natural reservoir for asfv. together with genetic differences, other factors might be involved in the differential susceptibility to asf observed among eurasian suids (sus scrofa) and african warthogs (phacochoerus africanus). preliminary evidence obtaine ... | 2020 | 33077775 |
| african swine fever in the lithuanian wild boar population in 2018: a snapshot. | the first cases of african swine fever (asf) were detected in the lithuanian wild boar population in 2014. since then, the disease spread slowly through the whole country, affecting both, wild boar and domestic pigs. in the other baltic states, which both are also affected by asf since 2014, the recent course of asf prevalence suggests that the countries might be well under way of disease elimination. in contrast, in lithuania the epidemic seems to be still in full progress. in the present study ... | 2020 | 33028388 |
| stability of african swine fever virus in carcasses of domestic pigs and wild boar experimentally infected with the asfv "estonia 2014" isolate. | europe is currently experiencing a long-lasting african swine fever (asf) epidemic, both in domestic pigs and wild boar. there is great concern that carcasses of infected wild boar may act as long-term virus reservoirs in the environment. we evaluated the tenacity of asf virus (asfv) in tissues and body fluids from experimentally infected domestic pigs and wild boar, which were stored on different matrices and at different temperatures. samples were analysed at regular intervals for viral genome ... | 2020 | 33019736 |
| rapid emergence of african swine fever virus variants with different numbers of a tandem repeat sequence in south korea. | african swine fever virus variants with different numbers of a 10-bp tandem repeat were isolated in south korea soon after being identified in wild boar. the short emergence periods and sympatric distributions within a narrow geographical region suggest that the variants were sporadically generated in the pre-existing viral population. | 2020 | 33012101 |
| persistent domestic circulation of african swine fever virus in tanzania, 2015-2017. | african swine fever (asf) is a highly fatal viral hemorrhagic disease of domestic pigs that threatens livelihoods and food security. in africa, asf virus (asfv) circulates in sylvatic (transmission between warthogs and soft argasid ticks) and domestic (transmission between domestic pigs) cycles, with outbreaks resulting from asfv spill-over from sylvatic cycle. a number of outbreaks were reported in different parts of tanzania between 2015 and 2017. the present study investigated asfv transmissi ... | 2020 | 33004025 |
| the spillover of african swine fever in western poland revealed its estimated origin on the basis of o174l, k145r, mgf 505-5r and igr i73r/i329l genomic sequences. | the african swine fever epidemic occurred in poland at the beginning of 2014 and, up to date, the disease has been spreading mainly in the eastern part of the country. unexpectedly, in november 2019 an infected wild boar case was confirmed in lubuskie voivodship in western poland. during the following weeks, several dozen african swine fever virus (asfv)-positive animals were notified in the neighboring area, causing severe concern regarding further spread of the disease to the mostly pig-dense ... | 2020 | 32992547 |
| african swine fever circulation among free-ranging pigs in sardinia: data from the eradication program. | african swine fever virus (asfv), the cause of a devastating disease affecting domestic and wild pigs, has been present in sardinia since 1978. in the framework of the regional asf eradication plan, 4484 illegal pigs were culled between december 2017 and february 2020. the highest disease prevalence was observed in the municipality with the highest free-ranging pig density, and culling actions drastically reduced asfv circulation among these animals. asfv-antibody were detected in 36.7% of teste ... | 2020 | 32967098 |
| identification of novel testing matrices for african swine fever surveillance. | african swine fever (asf) is a devastating viral disease of pigs and wild boar, and it threatens global food security. we aimed to identify suitable sample matrices for use in asf surveillance programs. six pigs inoculated with asfv were sampled at postmortem. blood, bone marrow, ear biopsies, and oral, nasal, and rectal swabs were taken from all pigs. all samples were analyzed using 3 real-time pcr (rtpcr) assays and a lamp assay. asfv was detected at > 107 genome copies/ml in blood; bone marro ... | 2020 | 32964810 |
| multifaceted immune responses to african swine fever virus: implications for vaccine development. | african swine fever (asf) is a highly contagious, often fatal viral disease caused by african swine fever virus (asfv), leading to high fever, severe hemorrhages with high lethality in domestic pigs and wild boar. in 2007, asf was reintroduced into europe. since then, asf has spread to many european and asian countries and now becomes a major concern to the swine industry worldwide. there have been various vaccine attempts, but no commercial asf vaccines are available so far. a key hurdle in dev ... | 2020 | 32932135 |
| porcine immunoglobulin fc fused p30/p54 protein of african swine fever virus displaying on surface of s. cerevisiae elicit strong antibody production in swine. | african swine fever virus (asfv) infects domestic pigs and european wild boars with strong, hemorrhagic and high mortality. the primary cellular targets of asfv is the porcine macrophages. up to now, no commercial vaccine or effective treatment available to control the disease. in this study, three recombinant saccharomyces cerevisiae (s. cerevisiae) strains expressing fused asfv proteins-porcine ig heavy chains were constructed and the immunogenicity of the s. cerevisiae-vectored cocktail asfv ... | 2020 | 32915442 |
| isolation and propagation of classical swine fever virus in porcine wharton's jelly mesenchymal stem cells. | classical swine fever (csf) is an extremely infectious and deadly disease of pigs and wild boars caused by the csf virus (csfv) which is a member of the pestivirus genus and the family flaviviridae. this study was designed to detect the permissibility and replication of csfv in mesenchymal stem cells (mscs) monolayer derived from porcine wharton's jelly. porcine wharton's jelly mscs (pwj-mscs) were ex vivo expanded and propagated for more than 81 generations and third passage pwj-mscs were chara ... | 2020 | 32885744 |
| clinical course and gross pathological findings in wild boar infected with a highly virulent strain of african swine fever virus genotype ii. | african swine fever (asf) is a notifiable disease that in recent years has spread remarkably in europe and asia. eurasian wild boar (sus scrofa) plays a key role in the maintenance and spread of the pathogen. here we examined gross pathology of infection in wild boar with a highly virulent, hemadsorbing genotype ii asf virus (asfv) strain. to this end, six wild boars were intramuscularly inoculated with the 10 had50 arm07 asfv strain, and 11 wild boars were allowed to come into direct contact wi ... | 2020 | 32842614 |