| school milk and growth in schoolchildren. | | 1978 | 80596 |
| search by immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii in crohn's disease. | crohn's-disease tissue was tested by indirect immunofluorescence for antigens of rotavirus, pseudomonas maltophilia, and mycobacterium kansasii. no reactions were obtained with anti-serum to p. maltophilia and m. kansasii, and a granular fluorescence seen with rotavirus antibody was probably non-specific. | 1978 | 80631 |
| cow's milk as cause of colic in breast-fed infants. | | 1978 | 80655 |
| ige antibodies to cow's milk in breast-fed babies. | | 1978 | 80656 |
| rift valley fever in egypt, 1978. | | 1978 | 80678 |
| the human rumen. | the cow is a ruminant, and cow's milk has evolved to promote bacterial growth in the upper small bowel; whereas human milk has evolved to discourage bacterial growth. examination of the constituents of the two milks shows that their differences can be accounted for in terms of this difference in function. children who are fed a calf's diet tend to develop a rumen. this may lead to chronic diarrhoea and malnutrition and may be a factor in diarrhoea ascribed to cow's-milk-protein allergy and lacto ... | 1978 | 80689 |
| further studies on amplification of cell-associated immunological memory by secondary antigenic stimulus. | | 1978 | 80739 |
| immunoglobulin levels in large calf agglomerations. | the zinc sulphate turbidity test was used to establish the total immunoglobulin levels of calves from birth to 4 months of age. colostral immunity fell rapidly and the lowest levels were found in the third and fourth week of life. during the fifth week there was a significant increase. 25% of animals were hypogammaglobulinaemic in the first week of life. this ratio increased to 50% in the third week of life. at the age of two months the lowest immunoglobulin levels almost vanished. these finding ... | 1978 | 80804 |
| effect of periodate oxidation on specific activities and carbohydrate components of human blood group n- and m-specific glycoproteins and glycopeptides. | mild as well as strong periodate oxidation of isolated erythrocyte n and m glycoproteins and glycopeptides gave extensive to complete destruction of n-specificities as measured with vicia graminea extracts and of n- as well as m- activities determined with all but 1 of 8 animal anti-n and 13 anti-m sera. results with human antisera differed somewhat, while the specificity of mildly oxidized n-glycoprotein was completely destroyed as determined with all 8 human anti-n sera used, that of strongly ... | 1978 | 80880 |
| [detection of common antigenic determinants in the envelopes of mammalian type d (mason--pfizer) and type c virions by using the virion precipitation reaction]. | immune sera to mason-pfizer virus (m-pmv) are capable of precipitating type c viruses of mice (nih-mulv, g-mulv, and to a lower degree r-mulv), cats (felv, rd-114), and monkeys (ssv-1, galv, bev). the immune sera to rd-114, bev, ssv-1 and galv viruses can precipitate m-pmv virions. the adsorption analysis suggests that cross-reactions between m-pmv, rd-114, nih-mulv, and g-mulv viruses depend on common virus-specific antigenic determinants in the envelopes of the virions. the relationship betwe ... | 1978 | 80888 |
| [essay of proteins after aldehyde treatment in biological objects (author's transl)]. | following fixation with formalin or glutaraldehyde, the protein content of bovine serum albumin, lysozyme, non-disintegrated yeast cells and liver nuclei of mice is determined by means of a brom-phenol blue staining procedure and the widely used lowry-technique. the bromphenol blue technique permits determinations of soluble as well as particulate proteins or protein mixtures fixed with up to 6% formalin or 2% glutaraldehyde. recovery rates differ no more than 20% as related to unfixed controls. ... | 1978 | 80908 |
| conformation of myelin basic protein and its role in myelin formation. | high resolution 13c and 1h nmr spectra of myelin basic protein over a range of ph and concentrations indicate that intramolecular folding of the polypeptide chain occurs in aqueous solution in the region of residues 85 to 116. at ph 4 in d2o solution, the 13c resonances due to nonprotonated carbons of phenylalanine and tryptophan are broadened and chemically shifted compared to the same resonances when the protein is dissolved in 6m guanidinium hydrochloride. these residues occur in the region o ... | 1978 | 80938 |
| cross-linking of lipid bilayers by central nervous system myelin basic protein: aggregation of free and vesicle-bound protein. | central nervous system myelin basic protein binds to the zwitterionic lipid, egg diacylphosphatidylcholine, over a wide range of ph and ionic strength. lipid vesicles containing the protein have been observed to increase in size and to aggregate. the size increase is most marked at very low ionic strengths whereas aggregation is evident at ionic strengths from 0.001 to 0.35. the ph and ionic-strength dependence of this aggregation closely follows that of the self-association of the protein, sugg ... | 1978 | 80939 |
| molecular organisation in central nerve myelin. | pertinent data from the literature and in press is summarised and used to construct a model for the molecular arrangement of lipid and protein in the lamellae of compact central nerve myelin. for the lipid phase of myelin the available data is best interpreted in terms of a bilayer arrangement while physical studies suggest that the lipids are in an intermediate fluid state maintained by the presence of cholesterol and water in the system. lipids will interact to maintain this condition. the pro ... | 1978 | 80940 |
| molecular bases for the difference in the potency of myelin basic protein from different species in lewis rats. | the results of this study define the chemical bases for the difference in the encephalitogenic potency reported for the bovine and guinea pig myelin basic proteins. studies with synthetic peptides showed that the sequence of peptide s53, h-ser-gln-arg-ser-gln-asp-glu-asn-oh, which is native to the guinea pig basic protein, is the minimum amino acid sequence necessary for inducing eae in lewis rats. the results of this study further showed that specific sequence modifications rendered the native ... | 1978 | 80942 |
| comparison of the rat and mouse encephalitogenic determinants. | | 1978 | 80943 |
| neutral proteinases secreted by macrophages degrade basic protein: a possible mechanism of inflammatory demyelination. | in the inflammatory demyelinating diseases, such as multiple sclerosis, landry-guillain-barré syndrome and experimental allergic encephalomyelitis, demyelination occurs in the vicinity of infiltrating mononuclear cells. although the histopathology is characteristic of each disease, the general observation that myelin destruction in inflammatory lesions begins prior to phagocytosis suggests a common mechanism for myelinolysis in these diseases. recent studies show that stimulated macrophages secr ... | 1978 | 80946 |
| studies on myelin proteins in human peripheral nerve. | the myelin fraction from human peripheral nerve was prepared. two basic protein fractions (bf-p2 and pb) were isolated from acid extracts of the myelin fraction and three glycoproteins (br-po, pasii and y protein) were purified from its acid-insoluble residue. in biochemical analysis the human bf-p2 protein (m.w.13,000) showed similar but not identical properties to bovine bf-p2 protein. the pb fraction was suggested to include the encephalitogenic cns-bp (m.w.18,000) and another, new protein of ... | 1978 | 80952 |
| cytochemical study of estrogen receptor in human mammary cancer. | a 17beta-estradiol-6-carboxymethyl-oxime-bovine serum albumin--fluorescein isothiocyanate conjugate is prepared by attaching on the average 11 moles of the fluorescein dye and 24 moles of the steroid hormone to each mole of the protein carrier. this fluorescent estradiol conjugate is used as a tracer to detect estrogen receptor of human mammary cancer cells in frozen sections. the cytochemical findings indicate that mammary carcinomas are composed of heterogeneous populations of receptor-positiv ... | 1978 | 80958 |
| polyacrylamide gel staining with fe2+-bathophenanthroline sulfonate. | | 1978 | 80962 |
| metachromasy of peripheral nerve collagen on polyacrylamide gels stained with coomassie brilliant blue r-250. | | 1978 | 80963 |
| gene clusters in the blood group system b of cattle. | in this report the linear order of genetic determinants for the blood group system b of cattle has been worked out in more detail. two groups of antigenic specificities--inclusion groups--showing serologic relationship are described. recombination frequencies do suggest that these specificities are controlled by two clusters of genetic determinants. the structures of these inclusion groups and clusters are compared with those of antigenic complexes in other species, especially in mice. several s ... | 1977 | 80965 |
| molecular localization of two cattle allotypic specificities. | | 1978 | 81049 |
| inhibition of cyclic amp phosphodiesterase by 2,6-dimethyl-4-(3-nitrophenyl)-1,4-dihydropyridine-3,5-dicarboxylic acid 3-[2-(n-benzyl-n-methylamino)] ethyl ester 5-methyl ester hydrochloride (yc-93), a potent vasodilator. | | 1978 | 81059 |
| mechanisms of tachyarrhythmias, past and present. | several hypotheses, developed in the early years of arrhythmology, and frequently used for the explanation of many types of clinical tachyarrhythmias, are often based on experiments on unusual models, unphysiologic interventions, or, in some instances, on erroneously interpreted histologic findings. however, it can be demonstrated that several of these hypotheses are valid. focal activity, unidirectional block, extremely slow conduction velocity, differences in conduction velocity in closely adj ... | 1978 | 81132 |
| binding bovine immunoglobulins to anterior lobe cells of the hypophysis. | cells of the anterior lobe of the bovine hypophysis, show an affinity for immunoglobulins igg, iga and igm. the phenomenon either provides a message to stimulate cellular secretion or to excite mechanisms associated with the synthesis and release of ig's or their determinants. | 1978 | 81138 |
| antibodies to dna modified by the carcinogen n-acetoxy-n-2-acetylaminofluorene. | | 1978 | 81140 |
| glial tissue in the periventricular nucleus and mamillary body in postnatal development in the cow. | | 1978 | 81162 |
| reactions of rabbit antisera to human beta2-microglobulin (beta2-m) with platelets. | reactions of rabbit antisera to human beta2-m against human platelets and man-mouse hybrid cells were studied by ma tests and cytolysis tests. evidence is presented that the beta2-m act as species-specific cell surface antigens, some of which are most likely shared by cell surface proteins determined by genes on chromosomes other than the chromosome 15. | 1978 | 81176 |
| identification and behavior of the precipitating blv antibodies in sera of leukotic cattle. | precipitating bovine leukemia virus antibodies isolated from bovine leukemic sera by ion-exchange chromatography, gel filtration and affinity chromatography were identified as immunoglobulins of the igg1 subclass and iga class. they proved to be different with regard to molecular size and electrical charge. immunoglobulins igg2 and igm as well as low-molecular components ranging in the microglobulin regions (less than 4s) failed to precipitate blv antigens. individual bovine leukemic sera showed ... | 1978 | 81182 |
| classification of rotaviruses: report from the world health organization/food and agriculture organization comparative virology program. | the reoviridae working team was established under the world health organization/food and agriculture organization comparative virology program in 1975. the generic name rotavirus has been adopted for the reovirus-like agents associated with diarrhea in man and animals, and the nebraska calf diarrheal virus strain of bovine rotavirus has been selected as a candidate reference virus. stocks of this virus and of gnotobiotic calf antiserum have been prepared. antigenic differences among rotaviruses ... | 1978 | 81198 |
| parvoviruses associated with diarrhea in calves. | | 1978 | 81199 |
| detection of goitrin and its heptafluorobutyryl derivative by gas-liquid chromatography with electron capture, electrolytic conductivity and sulfur detectors. | the separation of goitrin by two gas-liquid chromatographic columns, the response of different detectors and the use of the heptafluorobutyric (hfb) anhydride derivative of goitrin to improve sensitivity was investigated. the non-polar 3% ov-1 and the intermediate 4% se-30/6% sp-2401 on 80-100 mesh chromosorb w hp gave comparable results and were considered interchangeable. the sensitivities of the electron capture, sulfur 394 nm emission, chlorine and nitrogen coulson electrolytic conductivity ... | 1978 | 81212 |
| coagglutination test for identification of pasteurella multocida associated with hemorrhagic septicemia. | two serogroups (b and e) of pasteurella multocida associated with hemorrhagic septicemia in cattle were differentiated by a coagglutination test with antibody-coated staphylococci. the group antigen was soluble and heat stable. it was not detectable in unencapsulated cells or their heated extracts. adsorbed onto human type o erythrocytes, it could be demonstrated by an indirect hemagglutination test with specific antiserum or by coagglutination with specific antibody-coated staphylococci. antibo ... | 1978 | 81214 |
| cellular basis for a hapten-specific state of tolerance induced in vitro. | a hapten-specific unresponsive state was induced in vitro by the incubation of normal murine spleen cells with highly conjugated dinitrophenylated bovine gamma-globulin (dnp-bgg) or a dinitrophenylated copolymer of d-glutamic acid and d-lysine (dnp-d-gl) for 24 hr. after this incubation period spleen cells were washed and cultured for 4 days with the thymic-independent antigen dinitrophenylated polyacrylamide beads (dnp-paa) or the thymic-dependent antigen trinitrophenylated burro the erythrocyt ... | 1978 | 81228 |
| induction and mechanism of tolerance to bovine serum albumin in mice given total lymphoid irradiation (tli). | balb/c mice given total lymphoid irradiations (tli) were injected i.p. with bovine serum albumin (bsa) in saline, and challenged with dnp-bsa in complete freund's adjuvant 6 weeks later. the latter animals made no anti-dnp antibody response as measured by a modified farr assay, but made a normal anti-dnp response after challenge with dnp-bgg in adjuvant. normal mice or mice given whole body irradiation were not tolerized by the i.p. injection of bsa in saline. spleen cells from unresponsive mice ... | 1978 | 81232 |
| purification of functional, determinant-specific, idiotype-bearing murine t cells. | strain a/j mice immunized with azobenzenearsonate (aba)-mouse igg conjugates develop suppression for anti-trinitrophenyl(tnp) responses to doubly conjugated (aba,tnp) proteins. this suppression is specific for the aba epitope and is mediated by t cells in cell transfer experiments. aba-binding t cells from suppressed animals were purified by a two-stage procedure in which b cells were removed from spleen cell populations by adherence to plastic surfaces coated with anti-mouse ig antibody, follow ... | 1978 | 81261 |
| pre-lytic release of foot-and-mouth disease virus in cytoplasmic blebs. | the pre-lytic release mechanism of foot-and-mouth disease virus was investigated by immunofluorescence, acridine orange staining, and electron microscopy in infected bovine and porcine kidney coverslip cultures. cells with cytoplasmic fluorescence and which were positive for single stranded rna with acridine orange staining were observed at 2 h after infection. scanning electron microscopy showed cytoplasmic blebs in all cultures examined 2 h after infection. rounded cells with virus inclusions ... | 1978 | 81266 |
| bovine leukemia virus involvement in enzootic bovine leukosis. | | 1978 | 81603 |
| a simple method for quantitative, semiquantitative, and qualitative assay of protein. | | 1978 | 81625 |
| surface antigens of rat liver epithelial cells grown in medium containing foetal bovine serum. | cultured rat liver cells induced a strong antibody response in syngeneic rats, directed against foetal calf serum components which were incorporated into the liver cell surface from the cell-culture medium. this antibody could be removed by absorption with liver cells or glutaraldehyde-fixed foetal calf serum. it is possible that the antigenic cross-reactivity observed in earlier studies with cultured cells treated with carcinogens could be due to this foetal calf serum component. | 1978 | 81676 |
| the use of base pair specific dna binding agents as affinity labels for the study of mammalian chromosomes. | the fluorochromes hoechst 33258 and olivomycin are base pair specific dna binding agents. the fluorescence enhancement of hoechst 33258 and olivomycin in the presence of dna can be directly related to the a--t and g--c content of the interacting dna respectively. cytological observations of metaphase chromosomes treated with these two compounds suggest that the fluorescent banding patterns produced are the reverse of one another.--non-fluorescent base pair specific dna binding agents have been u ... | 1978 | 81737 |
| antibody as an immunological probe for studying the refolding of bovine serum albumin. an immunochemical approach to the identification of possible nucleation sites. | specifically purified antibody to either domain i, domain iii, or to subregions of domains i or iii of serum albumin was added to refolding mixtures containing reduced serum albumin but no other refolding catalyst. it was found that the refolding of reduced albumin was greatly enhanced by the presence of specific antibody in the refolding mixture, that this enhancement was restricted to that domain for which the added antibody was directed, and that antibody-mediated enhancement of refolding in ... | 1978 | 81833 |
| the antigenic structure of bovine serum albumin. evidence for multiple, different, domain-specific antigenic determinants. | using antiserum to native bovine albumin and antigenically active fragments of the protein, we have isolated antibodies directed to each of the three domains and to several subdomains of the albumin molecule. using albumin and these fragments as inhibitors of the reaction between 125i-albumin and any given antibody population, we have demonstrated that: (a) each domain of albumin is antigenically distinct from each of the other domains; (b) each domain possesses a minimum of two different antige ... | 1978 | 81834 |
| cross-linking studies on the conformation and dimerization of myelin basic protein in solution. | myelin basic protein was isolated from both cat and bovine central nervous system. cat and bovine myelin basic protein, which are shown to be similar by tryptic mapping, exhibit identical behavior when cross-linked with the bifunctional reagent difluorodinitrobenzene. myelin basic protein is cross-linked into only a dimer under certain conditions in the presence of sodium dodecyl sulfate. in contrast, many oligomers are formed when myelin basic protein is cross-linked in the absence of detergent ... | 1978 | 81835 |
| immunological studies on pancreatic phospholipase a2. antigenic characterization of the nh2-terminal region. | rabbit antisera elicited against pure pig, horse, ox, and sheep pancreatic phospholipase a2 revealed considerable immunological differences when tested by double immunodiffusion and microcomplement fixation assays. snake venom phospholipases did not show any detectable cross-reactions with the pancreatic enzymes. microcomplement fixation also clearly demonstrated conformational differences between porcine phospholipase a2 and its zymogen. nh2 terminally modified analogs of porcine phospholipase ... | 1978 | 81837 |
| effect of orally administered duodenal fluid on serum proteins in neonatal calves. | this study was to determine if orally administered duodenal fluid, as a source of intestinal microorganisms, would influence absorption of gamma-globulin of colostrum in newborn calves. duodenal fluid was obtained 2 h postfeeding from a cannulated milk-fed calf. twenty-seven male and female holstein calves obtained within 6 h of birth were assigned randomly to one of three treatments: 1) colostrum alone, 2) 200 ml duodenal fluid immediately followed by colostrum, and 3) 200 ml duodenal fluid fol ... | 1978 | 81840 |
| absorption of colostral proteins by newborn calves fed unfermented, fermented, or buffered colostrum. | unfermented, frozen colostrum from the first three postpartum milkings of 10 cows was thawed, pooled, and treated to produce three diets: 1) unfermented, 2) fermented (7 days at 25 to 27 c), and 3) fermented (as in 2) with ph adjusted to match that of unfermented colostrum. eighteen newborn, unsuckled holstein calves were assigned randomly to one of the three diets. colostrum diets were thawed and fed at 0, 8, 16, 24, and 36 h. blood was sampled at 0, 4, 8, 16, 24, and 48 h. minimal breakdown of ... | 1978 | 81841 |
| adrenal function and passive immunity in the dystocial calf. | dystocial calves were tested for their ability to absorb colostral immunoglobulins and establish a competent passive immunity. twenty dystocial and 20 eutocial calves were fed 1 liter of colostrum 4 h after birth and an additional liter 12 h later. their absorption of the immunoglobulins a, g, and m, as indicated by serum concentrations, was equivalent at 16 and 24 h postpartum. cortisol concentrations in serum at birth were high in both eutocial and dystocial calves and were correlated negative ... | 1978 | 81842 |
| complete versus selective dry cow therapy for mastitis control. | two hundred and thirty-two cows were assigned alternately to complete dry cow therapy (infusion in all quarters on the day of drying off) or selective therapy (infusion in all quarters if a history of mastitis, california mastitis test score of +2 or +3 in any quarter, or if cell counts from bucket milk samples as determined by the membrane filter-deoxyribonucleic acid procedure were above 500,000 cells/ml). a dry cow product containing 10(6) units of procaine penicillin g and 1 g of dihydrostre ... | 1978 | 81843 |
| studies of hypersensitivity reactions to foods in infants and children. | in order to extend previous investigations of adverse reactions to foods performed at this institution, 68 children, aged 5 mo to 15 yr, were studied. all subjects reported a history of adverse reaction to ingestion of one or more of the 14 foods under study. sixteen of 43 subjects, 3 yr of age or older, had 22 adverse reactions during 94 food challenges with one or more of the 14 foods. all reactions confirmed were to peanut or other nuts, milk, egg, and soy. skin testing with 1:20 weight/volum ... | 1978 | 81844 |
| immunochromatography on insolubilized antibodies of very low affinity: application to immunoadsorbence of bovine alpha-fetoprotein. | cross-reactive antibodies were utilized to prepare immunoadsorbents possessing a very low affinity to bovine alpha-fetoprotein (afp). a goat anti-human afp serum cross-reactive with bovine afp was first depleted of antibodies reactive with bovine afp in immunodiffusion. the remaining antibodies from this serum and gamma-globulin from a sheep antiserum against rabbit afp, without prior absorption, were coupled to sepharose. chromatography of fetal calf serum on these adsorbents resulted in retard ... | 1978 | 81850 |
| secretion rate independent evaluation of igm antibody avidity at the level of single immunocytes. | the hemolytic plaque inhibition assay has been performed on spleen cells from mice immunized with tnp-hrbc to evaluate avidity of anti-tnp igm antibodies. at different times after immunization direct plaques were inhibited by soluble tnp-eaca, tnp61-bgg, or anti-mu antiserum. analysis of the inhibition data provided independent estimates of antibody avidity and secretion rate. avidity was found to increase with time, to reach a maximum when the antibody response attained the peak value, and then ... | 1978 | 81861 |
| studies of immunosuppression by cobra venom factor. i. on early igg and igm responses to sheep erythrocytes and dnp-protein conjugates. | the immunosuppressive activity of cvf was evaluated in mice immunized with sheep erythrocytes and dinitrophenylated proteins. serum antibody levels to these immunogens were estimated in activity units and on a weight basis for igg. igm as well as igg antibody responses were diminished in mice pretreated with cvf. however, when soluble immunogens were incorporated in cfa the suppressive effect associated with cvf was inapparent. it is suggested that c depletion per se may not fully account for th ... | 1978 | 81862 |
| comparison of the developmental kinetics of antibody- and immunoglobulin-forming cells in normal and tolerant mice. | the developmental kinetics of antibody-forming cells (afc) and of cells synthesizing immunoglobulins without detectable antibody function against the antigen injected (ifc) were compared in lymph nodes of normal mice and tolerant mice receiving an immunogenic challenge. tolerant states to bovine serum albumin (bsa), induced in adult mice, and to human gamma-globulin (hgg), induced in newborn mice, were studied. whatever the tolerant state induced, the following main results were obtained: 1) bot ... | 1978 | 81865 |
| structure-activity relationships and specificity of inhibition of dna polymerases from normal and leukemia cells of man and from simian sarcoma virus by rifamycin derivatives. | | 1978 | 81880 |
| cot deaths and water-sodium. | over a thirty-year period in scunthorpe, during which there were two changes in the sodium content of the water supply, there were significantly more sudden deaths in infancy during the decade when sodium levels were highest. this is compatible with the hypothesis that hypernatraemia is a factor leading to cot death. changes in the incidence of breastfeeding over the same period may have contributed to, but are unlikely to account for, the result because the pattern was different in a neighbouri ... | 1978 | 82031 |
| the human rumen. | | 1978 | 82113 |
| trimethoprim-resistance plasmids and transposons in salmonella. | | 1978 | 82155 |
| interactions of phosphotungstate with biological polymers. | | 1975 | 82257 |
| [stimulation of dna synthesis in intact rat livers using serum beta globulins]. | | 1978 | 82274 |
| immunochemistry of bovine serum albumin. | two fragments were isolated from bsa one was derived from the first terminal third of the molecule and the second from the last third of the molecule. each fragment inhibited the reaction of bsa-anti bsa by 90% or better. an immunoabsorbent of each bound 90% of anti bsa. each fragment bound two antibody molecules per mole of fragment. these results are explained by the concept that bsa contains repeating identical or similar antigenic determinants. conformational non identity of various batches ... | 1978 | 82378 |
| evidence for complement activation following the oral administration of cow's urine concoction in rats. | oral administration of cow's urine concoction in rats causes the activation of the third complement component in the serum. the product of this activation has some histame-releasing effects and causes a characteristic acute fall in neutrophil and monocyte counts in the peripheral blood which is reversed within four hours. the importance of these observations to the areas of the world where cow's urine concoction is used for the treatment of childhood convulsion is discussed. | 1978 | 82393 |
| binding of antigen to surface ig on isolated rat mast cells. | this study concerns observations on isolated rat mast cells sensitized to specific antigen. specific binding of antigen to the surface immunoglobulins of these cells could be demonstrated by immunofluorescence technique as well as by radiolabelled antigen binding and the degree of binding parallelled the allergic reaction as judged by histamine release. exposure of mast cells to antigen at both low and high antigen concentrations did not change the distribution of the surface immunoglobulins. fu ... | 1978 | 82408 |
| separation of anti-histone antibodies from nonimmune histone-precipitating serum proteins, predominantly alpha2-macroglobulin. | | 1978 | 82424 |
| allergic encephalomyelitis: evidence for lack of significant encephalitogenic activity of purified peptide l in the monkey. | | 1978 | 82445 |
| bovine alpha-fetoprotein. isolation and characterization. | bovine afp was purified by ion exchange chromatograph on c.m. cellulose and deae sephadex a-50, gel filtration and immunosorbent technique. afp was homogeneous when studied by gel electrophoresis under non denaturing and denaturing conditions, by ultracentrifugation and by immunological methods. the following molecular data were obtained: 1. sedimentation equilibrium indicated a molecular weight of 66,500 and sedimentation velocity gave s degrees 20, w = 4.71 s. a partial specific volume v = 0.7 ... | 1978 | 82453 |
| covalent binding of cardiolipin to gamma-globulin and albumin depends on peroxidation. | | 1978 | 82491 |
| experimental allergic encephalitis: study of cellular immunity during disease suppression. | administration in complete freund's adjuvant of encephalitogenic protein (ep), derived from central nervous tissue to guinea pigs, regularly results in the development of experimental allergic encephalitis (eae) which leads to the death of the animals. administration of ep in incomplete freund's adjuvant at an appropriate time will completely suppress the clinical development of disease. results reported herein show that animals receiving suppressive injections of ep for 7 days show depression o ... | 1978 | 82509 |
| differential inhibition of dna polymerases of calf thymus by 9-beta-d-arabinofuranosyladenine-5'-triphosphate. | the effect of 9-beta-d-arabinofuranosyladenine-5'-triphosphate (araatp) on the reactions of dna polymerases alpha and beta [e.c. 2.7.7.7] purified from calf thymus was examined. the reaction of dna polymerase alpha was shown to be more sensitive to the inhibition than that of dna polymerase beta. the k1 value of dna polymerase beta for araatp was 45 micrometer; 15 times higher than that of dna polymerase alpha (3 micrometer). the mode of inhibition by araatp was essentially competitive to deoxya ... | 1978 | 82555 |
| the use of gel filtration to follow conformational changes in proteins. conformational flexibility of bovine myelin basic protein. | the hydrodynamic behavior of bovine myelin basic protein was studied by gel filtration through sephadex g-100 under conditions which included variations in ph from 2 to 12, variations in ionic strength from 0.01 to 1.5 m at ph 2 and from 0.1 to 2 m at ph 7, and variations in guanidinium chloride concentration from 0 to 6 m. a number of well characterized compact globular proteins were subjected to the same conditions for comparison. compact globular proteins showed major conformational transitio ... | 1978 | 82559 |
| prostaglandins and slow-reacting substance. | | 1979 | 82564 |
| endogenous thyrotropin-releasing hormone in the anterior pituitary: sites of activity as identified by immunocytochemical staining. | thyrotropin-releasing hormone (trh) immunoreactivity was localized in the rat anterior pituitary with rabbit anti-trh sera and the unlabeled antibody peroxidase-antiperoxidase complex (pap) technique. stain was present in secretory granules of cells possessing morphological characteristics of thyrotropes, gonadotropes and lactotropes. antibody absorption studies with anti-trh sera absorbed with trh, 3 diastereoisomeric analogues of trh, gonadotropin-releasing hormone (gnrh), bovine serum albumin ... | 1978 | 82570 |
| masking of protein antigen by modification of amino groups with carbobenzoxychloride (benzyl chloroformate) and demasking by treatment with nonspecific protease. | cryostat sections of various substrates were treated with carbobenzoxychloride in acetone to modify antigens. by applying specific fluorescent antibodies, it could be shown that the antigenic determinants of rabbit gamma-globulin and bovine insulin were totally masked. the antigenicity of acth was markedly reduced, whereas the polysaccharide antigens of salmonella typhimurium were only partially masked. after masking, antigenicity could be restored by treatment with nonspecific protease. the rev ... | 1978 | 82572 |
| autoantibodies to parathyroid hormone receptor. | autoantibodies which block the binding of parathyroid hormone to membrane receptors for the hormone were detected in the sera (especially in the igg fraction) of 49 out of 50 uraemic patients with secondary hyperparathyroidism (patients with high levels of c-regional parathyroid hormone). these antibodies are species-specific. their presence in the serum in unaffected by dialysis. inhibition of binding appears to be related to the rise in c-regional parathyroid-hormone levels and the duration of ... | 1978 | 82734 |
| inhibition of pancreas and gallbladder by pancreatic polypeptide. | stimulated pancreatic and biliary outputs were studied in seven healthy subjects during intravenous infusion of bovine pancreatic polypeptide (p.p.) (mean dose 65 pmol/kg/h). p.p. significantly inhibited outputs of trypsin and bilirubin at plasma concentrations similar to those observed after meals. in four cholecystectomised subjects, p.p. inhibited only trypsin output. | 1978 | 82783 |
| serum stimulates the na+,k+ pump in quiescent fibroblasts by increasing na+ entry. | two ionophores (monensin and gramicidin) that carry na+ into 3t3 cells markedly enhance the rate of 86rb+ uptake. ouabain prevents both ionophores from increasing 86rb+ uptake, indicating that the ionophores activate the na+,k+ pump. measurements of 86rb+ uptake and cell na+ and k+ over a range of monensin concentrations show that the activity of the na+,k+ pump in 3t3 cells is limited by the supply of internal na+ and is extremely sensitive to small changes in internal na+. serum rapidly enhanc ... | 1978 | 82969 |
| binding activity regulation of rabbit skeletal muscle adenosine 3'-5'-monophosphate-dependent protein kinases. | a camp-dependent protein kinase has been isolated from rabbit muscle and purified. the affinity constant of the enzyme for the nucleotide is ka = 9.3 x 10(-9) m, with a vmax = 0.013 x 10(12) moles bound camp/1 microgram protein. the influence exerted by different factors is studied: a) inhibitor (i) of kinase activity: increases the binding capacity for camp, by percentages which depend on the amount of i. in the presence of inhibitor (120 microgram/100 microliter) the affinity constant is ka = ... | 1978 | 82984 |
| staining of glycosaminoglycans of intervertebral disc tissue. | intervertebral discs of six species were stained with alcian blue and pas methods to identify glycosaminoglycans. the polyanions are most concentrated in 'ring-like' pericellular areas and in material lying between individual lamellae and bundles of collagen. a slight increase occurs early in life and towards the disc centre. the concentration of glycosaminoglycans cannot be correlated with species which are known to suffer from clinical disc disease, though there appears to be a higher concentr ... | 1978 | 82985 |
| transitivity of response in the mixed lymphocyte culture test. | we present a model of mixed lymphocyte culture (mlc) response which assumes one specificity per locus. it also assumes that an animal a will fail to stimulate animal b if, and only if, the set of specificities possessed by a is a subset of the set of specificities in b. the last assumption implies that non-stimulation is transitive; that is, if a does not stimulate b, and b does not stimulate c, then a will not stimulate c. the inclusion of antigenic sets can be used to partially order the anima ... | 1978 | 83014 |
| [histology of the oviduct ampulla epithelium in heifers following administration of melengestrol acetate]. | | 1978 | 83048 |
| antigenic properties and heterospecific antiviral activities of human leukocyte interferon species. | human interferon obtained in peripheral leukocytes was purified approximately 1000-fold by affinity chromatography on anti-leukocyte interferon globulins coupled to sepharose 4b, and by filtration on sds-sephadex g-100. the interferon was subsequently resolved into two molecular species by adsorption chromatography on sds-hydroxylapatite. the two species which were eluted at different phosphate molarities from hydroxylapatite, could also be distinguished on the basis of electric charge propertie ... | 1978 | 83098 |
| charge-dependent staining of proteins after electrophoretic separation in polyacrylamide gels. | | 1978 | 83114 |
| [electron paramagnetic resonance study of the interactions between steroid hormones and binding proteins]. | interaction of a spin labeled corticosteroid (desoxycorticosterone nitroxyde: doc -no) with three purified proteins (albumin, transcortin, progesterone binding protein: pbg) was studied by electron spin resonance (esr) spectroscopy. doc-no was competitive with natural corticosteroids and therefore bound at the same site to specific binding proteins. esr spectra in the presence of each of the proteins showed an immobilized (bound) form of the spin labeled steroid and allowed the calculation of th ... | 1978 | 83166 |
| the alternative complement pathway in bovine serum: the isolation of a serum protein with factor b activity. | | 1978 | 83291 |
| experimental allergic encephalomyelitis: sequestered encephalitogenic determinant in the bovine myelin basic protein. | | 1979 | 83353 |
| [prevention of development of experimental allergic encephalomyelitis by encephalitogenic protein]. | | 1978 | 83596 |
| a permanent cell viability assay using alcian blue. | the alcian blue dye exclusion method for glutaraldehyde-fixed cells has been utilized with "centrifugal cytology" to prepare permanent records of the viability of individual cells present in suspensions. the viability of spleen cell suspensions separated by linear bovine serum albumin density gradient centrifugation has been measured with this method. combined light and scanning electron microscopy of nonviable and viable cells demonstrated membrane alterations in alcian blue-stained nonviable c ... | 1978 | 83690 |
| cattle lymphocyte antigens. | | 1978 | 83723 |
| [immuno-enzymatic estimation of steroids (author's transl)]. | the authors present a reliable and sensitive estimation of progesterone using a conjugate progesterone iialpha-hémimaleate-beta galactosidase to identify the immunological reaction, the antibodies being obtained from the rabbit by means of a progesterone 11alpha-hemisuccinate bovine serum albumin. the free and bound fractions are separated by addition of rabbit antigamma globulin antibodies bound on glass beads by glutaraldehyde. this method is applied to estimations of cortisol and oestrone. | 1978 | 83806 |
| dynamic dialysis utilizing a hollow fibre unit as a rapid method for studying protein binding. | a useful approach for determining the binding ability which occurres in a protein-small molecule system was studied by a method of dynamic dialysis utilizing a hollow fibre unit. a dependency on experimental variables in the absence of protein was examined in order to characterize the nature of the dialytic process. it was shown that the rate of small molecule escaped across the dialytic membrane was dependent on stirring or flowing rate, temperature and ph, but independent on buffer strength or ... | 1978 | 83820 |
| intracellular resistivity and protein content [proceedings]. | | 1978 | 83835 |
| antigenic specificity and cellular mechanisms in leukocyte adherence inhibition analysis of immunity to simple proteins and hapten-protein conjugates. | the hemacytometer leukocyte adherence inhibition (lai) assay was investigated with respect to immunological relevance, specificity, and cellular mechanisms. humans were immunized to keyhole limpet hemocyanin, and rats were immunized to dinitrophenyl-bovine gamma-globulin. lai analysis disclosed classic patterns of immune response kinetics. the lai response was dose dependent in vitro with no inhibition at relatively high antigen doses. in vitro specificity in rats was restricted to the immunizin ... | 1979 | 83911 |
| lymphocyte specificity to protein antigens. ii. fine specificity of t-cell activation with cytochrome c and derived peptides as antigenic probes. | murine t-lymphocyte specificity was determined in a system of antigen driven in vitro t-cell proliferation using cytochrome c molecules from different species, their derived peptides and reconstituted hybrid proteins. it was observed that primed t cells could discriminate between peptide fragments which differed from each other at a single amino acid residue. these conclusions were substantiated by the pattern of cross-reactivity noted in the response of closely related cytochrome c proteins as ... | 1979 | 84044 |
| antibodies to galactocerebroside bind to oligodendroglia in suspension culture. | antisera raised in rabbits against galactocerebroside bind to bovine oligodendroglia in suspension in significant titer as demonstrated by indirect immunofluorescence. absorption of antigalactocerebroside antiserum with galactocerebroside, oligodendroglia, or myelin markedly reduces the antigalactocerebroside antibody titer as measured by a radioimmunoprecipitation test as well as the binding to oligodendroglia. incubation with some other galactose-containing glycolipids results in a parallel d ... | 1979 | 84053 |
| lymphocyte activity and serum-ige in relation to feeding in infants. | | 1979 | 84102 |
| dietary carbohydrate and metabolism of ingested protein. | six normal people were fed a lean beef meal while on a normal diet and after seven days on a very low carbohydrate (greater than 25 g/day) 2000 kcal/day diet. after carbohydrate restriction, the protein-induced rise in branched chain aminoacids was 40-50% greater than the rise after the control diet. intravenous leucine also produced a 40% greater rise in plasma-leucine after carbohydrate restriction. three days of fasting exaggerated protein-induced increases in plasma branched-chain aminoacids ... | 1979 | 84127 |
| deficiency of dihydrobiopterin synthetase. | | 1979 | 84304 |
| basic study on cerebral vasospasm: ca2+ movement during contraction-relaxation of isolated basilar arteries. | | 1979 | 84355 |
| brain-derived fibroblast growth factor: identity with a fragment of the basic protein of myelin. | fibroblast growth factors (fgf) isolated from bovine brain have been identified chemically and immunologically as components of the myelin basic protein. the intact bovine basic protein molecule (170 residues), prepared by the standard acid extraction procedure, lacked mitogenic activity (tested at concentrations up to 10 microgram/ml). however, the polypeptide fgf-2, identified as residues 44-153 of the basic protein, was maximally mitogenic for fibroblasts at 10 ng/ml and polypeptide 44-166 (f ... | 1978 | 84383 |