| temperature dependence of protein motions in a thermophilic dihydrofolate reductase and its relationship to catalytic efficiency. | we report hydrogen deuterium exchange by mass spectrometry (hdx-ms) as a function of temperature in a thermophilic dihydrofolate reductase from bacillus stearothermophilus (bs-dhfr). protein stability, probed with circular dichroism, established an accessible temperature range of 10 degrees c to 55 degrees c for the interrogation of hdx-ms. although both the rate and extent of hdx are sensitive to temperature, the majority of peptides showed rapid kinetics of exchange, allowing us to focus on pl ... | 2010 | 20534574 |
| proanthocyanidins from the leaves of machilus philippinensis. | seven proanthocyanidins (2-8) together with epicatechin (1) were isolated from the etoh extract of the leaves of machilus philippinensis. of these, machiphilitannins a (7) and b (8) are new natural products, with respective ic(50) values of 31.3 (7) and 18.4 microm (8) against alpha-glucosidase type iv from bacillus stearothermophilus. their structures were elucidated mainly on the basis of cd and 2d nmr analyses. in addition, aesculitannin b (2) showed inhibitory activity against alpha-glucosid ... | 2010 | 20568785 |
| a novel thermostable carboxylesterase from geobacillus thermodenitrificans: evidence for a new carboxylesterase family. | a novel gene encoding an esterase from geobacillus thermodenitrificans strain cmb-a2 was cloned, sequenced and functionally expressed in escherichia coli m15. sequence analysis revealed an open reading frame of 747 bp corresponding to a polypeptide of 249 amino acid residues (named estgta2). after purification, a specific activity of 2.58 u mg(-1) was detected using p-np caprylate (c8) at 50 degrees c and ph 8.0 (optimal conditions). the enzyme catalyses the hydrolysis of triglycerides (tributyr ... | 2010 | 20587647 |
| alpha-glucosidase inhibitory activity of bromophenol purified from the red alga polyopes lancifolia. | a bromophenol, bis(2,3-dibromo-4,5-dihydroxybenzyl) ether, was purified from the red alga polyopes lancifolia. its ic(50) values were 0.098 and 0.120 microm against saccharomyces cerevisiae and bacillus stearothermophilus alpha-glucosidases, respectively, and 1.00 and 1.20 mm against rat-intestinal sucrase and maltase. this bromophenol competitively inhibited s. cerevisiae alpha-glucosidase with a k(i) value of 0.068 microm and was very stable at ph 2 for 60 min at 37 degrees c. therefore, this ... | 2010 | 20629879 |
| extracellular secretion in bacillus subtilis of a cytoplasmic thermostable beta-galactosidase from geobacillus stearothermophilus. | beta-galactosidase catalyzes the hydrolysis of beta-galactosides into monosaccharides and is widely used in dairy processing. this study reports the extracellular secretion of a cytoplasmic thermostable beta-galactosidase from geobacillus stearothermophilus iam11001 in bacillus subtilis. this enzyme has potential applications in the dairy industry. it was not secreted in b. subtilis by mediation of 3 general secretory signal peptides, but was secreted extracellularly when it was fused to a twin- ... | 2010 | 20630200 |
| use of a mixture of surrogates for infectious bioagents in a standard approach to assessing disinfection of environmental surfaces. | we used a mixture of surrogates (acinetobacter baumannii, mycobacterium terrae, hepatitis a virus, and spores of geobacillus stearothermophilus) for bioagents in a standardized approach to test environmental surface disinfectants. each carrier containing 10 microl of mixture received 50 microl of a test chemical or saline at 22 +/- 2 degrees c. disinfectant efficacy criteria were > or = 6 log(10) reduction for the bacteria and the spores and > or = 3 log(10) reduction for the virus. peracetic ac ... | 2010 | 20639366 |
| fluorescence energy transfer in the bi-fluorescent s-layer tandem fusion protein ecfp-sgse-yfp. | this work reports for the first time on the fabrication of a bi-functional s-layer tandem fusion protein which is able to self-assemble on solid supports without losing its functionality. two variants of the green fluorescent protein (gfp) were genetically combined with a self-assembly system having the remarkable opportunity to interact with each other and act as functional nanopatterning biocoating. the s-layer protein sgse of geobacillus stearothermophilus nrs 2004/3a was fused with the cyan ... | 2010 | 20650318 |
| cytotoxicity and membrane interaction of tamoxifen as affected by ca(2+) and mg(2+): use of a bacterial model system. | a strain of bacillus stearothermophilus was used as a model to study the interaction of tamoxifen (tam) with the membrane and the cytostatic antiproliferative effects not related to estrogen binding. tam inhibits the growth of b. stearothermophilus as a function of concentration. the supplementation of the growth medium with ca(2+) or mg(2+) partially relieves the growth inhibition by tam, allowing growth at tam concentrations that fully impair growth in the basal medium. fluorescence polarizati ... | 1999 | 20654520 |
| expression and assembly of recombinant surface layer proteins in saccharomyces cerevisiae. | most bacterial surface layers (sls) are formed by self-assembly of a single type of protein. native and recombinant surface layer monomers are capable to self-assemble on solid substrates and in solution to highly regular nanosized arrays which make them attractive for nanobiotechnological applications. in this study, we expressed the surface layer protein sbsc of bacillus stearothermophilus attc 12980, tagged with enhanced green fluorescent protein, in the yeast saccharomyces cerevisiae. we obs ... | 2011 | 20658344 |
| a group iic-type intron interrupts the rrna methylase gene of geobacillus stearothermophilus strain 10. | group iic introns insert next to the stem-loop structure of rho-independent transcription terminators, thus avoiding intact genes. the insertion sites of 17 copies of the g.st.i1 intron from geobacillus stearothermophilus were compared. one copy of the intron was found to interrupt an open reading frame (orf) encoding an rrna methylase. | 2010 | 20675491 |
| improving the thermostability of geobacillus stearothermophilus xylanase xt6 by directed evolution and site-directed mutagenesis. | protein engineering of the thermostable xylanase xt6 from geobacillus stearothermophilus was performed to obtain enzymes with improved thermal tolerance. mutants producing such enzymes were obtained after several rounds of directed evolution using error-prone pcr and sequence family shuffling, in combination with a consensus-based semi-rational approach. the most thermostable mutant enzyme contained 13 amino acid substitutions and its half-life of inactivation was 52-fold of that of the wild-typ ... | 2010 | 20691586 |
| use of model micro-organism to study cytostatics-membrane interactions. | a strain of bacillus stearothermophilus was used as a model system to elucidate the interaction of tamoxifen with the cell membrane. this drug inhibits bacterial growth in a concentration-dependent manner. the inhibition increases at suboptimal and supraoptimal temperatures and is attenuated by the addition of ca(2+) to the growth medium. opposed physical alterations on bacterial membranes were observed when tamoxifen was added to the growth medium (increase in structural order) or directly in l ... | 1994 | 20692967 |
| bleach-boosting effect of crude xylanase from bacillus stearothermophilus sdx on wheat straw pulp. | pretreatment of wheat straw pulp using cellulase-free xylanase produced from bacillus stearothermophilus sdx at 60°c for 120min resulted in 4.75% and 22.31% increase in brightness and whiteness, respectively. enzyme dose of 10u/g of oven dried pulp at ph 9 decreased the kappa number and permanganate number by 7.14% and 5.31%, respectively. further chlorine dioxide and alkaline bleaching sequences (cded(1)d(2)) resulted in 1.76% and 3.63% increase in brightness and whiteness, respectively. enzyma ... | 2011 | 20709630 |
| pcra helicase dismantles reca filaments by reeling in dna in uniform steps. | translocation of helicase-like proteins on nucleic acids underlies key cellular functions. however, it is still unclear how translocation can drive removal of dna-bound proteins, and basic properties like the elementary step size remain controversial. using single-molecule fluorescence analysis on a prototypical superfamily 1 helicase, bacillus stearothermophilus pcra, we discovered that pcra preferentially translocates on the dna lagging strand instead of unwinding the template duplex. pcra anc ... | 2010 | 20723756 |
| evaluation of substrate promiscuity of an l-carbamoyl amino acid amidohydrolase from geobacillus stearothermophilus cect43. | n-carbamoyl-amino-acid amidohydrolase (also known as n-carbamoylase) is the stereospecific enzyme responsible for the chirality of the d- or l-amino acid obtained in the "hydantoinase process." this process is based on the dynamic kinetic resolution of d,l-5-monosubstituted hydantoins. in this work, we have demonstrated the capability of a recombinant l-n-carbamoylase from the thermophilic bacterium geobacillus stearothermophilus cect43 (bslcar) to hydrolyze n-acetyl and n-formyl-l-amino acids a ... | 2010 | 20730754 |
| human pathogens, nosocomial infections, heat-sensitive textile implants, and an innovative approach to deal with them. | implantable polymers, as used for biomedical applications, inherently have to be sterile. nonetheless, most implants, particularly those comprised of biomaterials developed in recent years for tissue engineering, are heat sensitive. therefore, use of hazardous (radio)chemicals--due to lack of alternative methods--is still state of the art for sterilization processes. the drawbacks of these techniques, both drastic and well known, lead to the demand for an alternative sterilization method, which ... | 2011 | 20824488 |
| hiv-1 gag p17 presented as virus-like particles on the e2 scaffold from geobacillus stearothermophilus induces sustained humoral and cellular immune responses in the absence of ifnγ production by cd4+ t cells. | we have constructed stable virus-like particles displaying the hiv-1 gag(p17) protein as an n-terminal fusion with an engineered protein domain from the geobacillus stearothermophilus pyruvate dehydrogenase subunit e2. mice immunized with the gag(p17)-e2 60-mer scaffold particles mounted a strong and sustained antibody response. antibodies directed to gag(p17) were boosted significantly with additional immunizations, while anti-e2 responses reached a plateau. the isotype of the induced antibodie ... | 2010 | 20850858 |
| [recombinant dna-methyltransferase m1.bst19i from bacillus stearothermophilus 19: purification, propeties and amino acids sequence analysis]. | the m1.bst19i dna-methyltransferase gene from restriction-modification system bst19i (recognition sequence 5'-gcatc-3') in bacillus stearothermophilus 19 has been cloned in the expressing vector pjw, that carries a tandem of thermo inducible promoters p(r)/p(l) from phage lambda. highly purified enzyme has been isolated by chromatography on various resins from e. coli cells where it accumulates in a soluble form. study of m1.bst19i properties has revealed that enzyme has a temperature optimum 50 ... | 2010 | 20873229 |
| structural basis for proficient incorporation of dttp opposite o6-methylguanine by human dna polymerase iota. | o(6)-methylguanine (o(6)-methylg) is highly mutagenic and is commonly found in dna exposed to methylating agents, even physiological ones (e.g. s-adenosylmethionine). the efficiency of a truncated, catalytic dna polymerase ι core enzyme was determined for nucleoside triphosphate incorporation opposite o(6)-methylg, using steady-state kinetic analyses. the results presented here corroborate previous work from this laboratory using full-length pol ι, which showed that dttp incorporation occurs wit ... | 2010 | 20961860 |
| mechanism of xylobiose hydrolysis by gh43 β-xylosidase. | glycoside hydrolases cleave the glycosidic linkage between two carbohydrate moieties. they are among the most efficient enzymes currently known. β-xylosidases from glycoside hydrolase family 43 hydrolyze the nonreducing ends of xylooligomers using an inverting mechanism. although the general mechanism and catalytic amino acid residues of β-xylosidases are known, the nature of the reaction's transition state and the conformations adopted by the glycon xylopyranosyl ring along the reaction pathway ... | 2010 | 20973564 |
| revisiting the hand wipe versus gel rub debate: is a higher-ethanol content hand wipe more effective than an ethanol gel rub? | the centers for disease control and prevention's guidelines for hand hygiene state that the use of alcohol-based hand wipes is not an effective substitute for the use of an alcohol-based hand rub or handwashing with an antimicrobial soap and water. the objective of this study was to determine whether a hand wipe with higher ethanol content (65.9%) is as effective as an ethanol hand rub or antimicrobial soap in removing bacteria and spores from hands. | 2010 | 21034977 |
| characterization of a dockerin-based affinity tag: application for purification of a broad variety of target proteins. | cellulose, a major component of plant matter, is degraded by a cell surface multiprotein complex called the cellulosome produced by several anaerobic bacteria. this complex coordinates the assembly of different glycoside hydrolases, via a high-affinity ca(2+)-dependent interaction between the enzyme-borne dockerin and the scaffoldin-borne cohesin modules. in this study, we characterized a new protein affinity tag, δdoc, a truncated version (48 residues) of the clostridium thermocellum cel48s doc ... | 2010 | 21038354 |
| characterization of statistically produced xylanase for enrichment of fruit juice clarification process. | critical factors for xylanase production of bacillus stearothermophilus under batch fermentation and for clarification of citrus fruit juice using this xylanase were optimized through central composite design of response surface methodology. statistical approach resulted in an increase of 1.19-fold in xylanase yield over conventional method. model equation for juice clarification included independent variables viz. temperature, incubation time and enzyme dose to study the dependent variables suc ... | 2010 | 21093618 |
| production of d-tagatose, a low caloric sweetener during milk fermentation using l-arabinose isomerase. | lactobacillusdelbrueckii subsp. bulgaricus and streptococcus thermophilus are used for the biotransformation of milk in yoghurt. during milk fermentation, these lactic acid bacteria (lab) hydrolyze lactose producing a glucose moiety that is further metabolized and a galactose moiety that they are enable to metabolize. we investigated the ability of l. bulgaricus and s. thermophilus strains expressing a heterologous l-arabinose isomerase to convert residual d-galactose to d-tagatose. the bacillus ... | 2010 | 21111612 |
| crystal structure of unliganded trap: implications for dynamic allostery. | allostery is vital to the function of many proteins. in some cases, rather than a direct steric effect, mutual modulation of ligand binding at spatially separated sites may be achieved through a change in protein dynamics. thus changes in vibrational modes of the protein, rather than conformational changes, allow different ligand sites to communicate. evidence for such an effect has been found in trap (trp rna-binding attenuation protein), a regulatory protein found in species of bacillus. trap ... | 2011 | 21175426 |
| spectroscopic studies and antibacterial activities of some new 16-membered octaazamacrocyclic complexes derived from thiocarbohydrazide and pentane-2,4-dione. | a series of macrocyclic complexes of the type [m(c12h20n8s2)x2]; where m=co(ii), ni(ii), cu(ii), zn(ii); x=cl-, no3-, ch3coo- has been synthesized by template condensation of thiocarbohydrazide and pentane-2,4-dione in the presence of divalent metal salts in methanolic medium. the complexes have been characterized with the help of elemental analyses, conductance measurements, magnetic measurements, electronic, nmr, ir, epr and ms spectral studies. the low value of molar conductance indicates the ... | 2010 | 21208826 |
| calcium dependent formation of tubular assemblies by recombinant s-layer proteins in vivo and in vitro. | surface layer proteins have the appealing property to self-assemble in nanosized arrays in solution and on solid substrates. in this work, we characterize the formation of assembly structures of the recombinant surface layer protein sbsc of geobacillus stearothermophilus attc 12980, which was tagged with enhanced green fluorescent protein and expressed in the yeast saccharomyces cerevisiae. the tubular structures formed by the protein in vivo are retained upon bursting the cells by osmotic shock ... | 2011 | 21258149 |
| strong and consistently synergistic inactivation of spores of spoilage-associated bacillus and geobacillus spp. by high pressure and heat compared with inactivation by heat alone. | the inactivation of spores of four low-acid food spoilage organisms by high pressure thermal (hpt) and thermal-only processing was compared on the basis of equivalent thermal lethality calculated at a reference temperature of 121.1°c (f(z)(121.1)(°)(c, 0.1 mpa or 600 mpa)) and characterized as synergistic, not different or protective. in addition, the relative resistances of spores of the different spoilage microorganisms to hpt processing were compared. processing was performed and inactivation ... | 2011 | 21278265 |
| a mixed-species microarray for identification of food spoilage bacilli. | failure of food preservation is frequently caused by thermostable spores of members of the bacillaceae family, which show a wide spectrum of resistance to cleaning and preservation treatments. we constructed and validated a mixed-species genotyping array for 6 bacillus species, including bacillus subtilis, bacillus licheniformis, bacillus pumilus, bacillus sporothermodurans, bacillus cereus and bacillus coagulans, and 4 geobacillus species, including geobacillus stearothermophilus, geobacillus t ... | 2010 | 21315980 |
| correlation between thermostability and stability of glycosidases in ionic liquid. | the activity and stability of a ß-glycosidase (thermus thermophilus) and two a-galactosidases (thermotoga maritima and bacillus stearothermophilus) were studied in different hydrophilic ionic liquid (il)/water ratios. for the ils used, the glycosidases showed the best stability and activity in 1,3-dimethylimidazolium methyl sulfate [mmim][meso(4)] and 1,2,3-trimethylimidazolium methyl sulfate [tmim][meso(4)]. a close correlation was observed between the thermostability of the enzymes and their s ... | 2011 | 21331585 |
| use of soybean vinasses as a germinant medium for a geobacillus stearothermophilus atcc 7953 sterilization biological indicator. | a novel low-cost medium was developed from by-products and wastes from the ethanol agro-industry to replace commercial media in the production of a steam sterilization biological indicator (bi). various recovery media were developed using soybean or sugarcane molasses and vinasse to prepare a self-contained bi. media performance was evaluated by viability and heat resistance (d(121 °c) value) according to regulatory standards. a medium produced with a soybean vinasse ratio of 1:70 (1.4%) (w/v) p ... | 2011 | 21336685 |
| enhancement of the hydrolysis activity of ß-galactosidase from geobacillus stearothermophilus by saturation mutagenesis. | thermostable ß-galactosidase (bgab) from geobacillus stearothermophilus is characterized by its thermoactivity in the hydrolysis of lactose to produce lactose-free milk products. however, bgab has limited activity toward lactose. we established a method for screening evolved mutants with high hydrolysis activity based on prediction of substrate binding sites. seven amino acid residues were identified as candidates for substrate binding to galactose. to study the hydrolysis activity of these resi ... | 2011 | 21338783 |
| nmr structure of the c-terminal domain of a tyrosyl-trna synthetase that functions in group i intron splicing. | the mitochondrial tyrosyl-trna synthetases (mt tyrrss) of pezizomycotina fungi are bifunctional proteins that aminoacylate mitochondrial trna(tyr) and are structure-stabilizing splicing cofactors for group i introns. studies with the neurospora crassa synthetase (cyt-18 protein) showed that splicing activity is dependent upon pezizomycotina-specific structural adaptations that form a distinct group i intron-binding site in the n-terminal catalytic domain. although cyt-18's c-terminal domain also ... | 2011 | 21438536 |
| detection of maduramycin residues in the tissues of chickens and pheasants by the screening test for antibiotic residues (star). | maduramycin is a coccidiostat authorized as a feed additive in poultry. council directive 96/23/ec stipulates that monitoring of foods of animal origin for residues of coccidiostats is mandatory. the aim of the present study was to evaluate the star for the screening of maduramycin residues in the tissues of broiler chickens and pheasants. both animal species were supplied feed medicated with cygro l% premix according to recommendations for use (5 mg kg(-1) of complete feed). the residues were i ... | 2011 | 21442501 |
| characterization of a bacterial self-assembly surface layer protein and its application as an electrical nanobiosensor. | bacterial cell surface layers (s-layers) are one of the most common outermost cell envelope components of prokaryotic organisms (archaea and bacteria), which can self-assemble into two-dimensional (2d) crystalline arrays. production and characterization of the bacterial s-layer protein (slp) from geobacillus stearothermophilus, a thermophilic bacterium, are demonstrated in this study. based on this, purified slps were applied for wrapping around single-walled carbon nanotubes (cnts) and applying ... | 2011 | 21446464 |
| the l-arabinan utilization system of geobacillus stearothermophilus. | geobacillus stearothermophilus t-6 is a thermophilic soil bacterium that has a 38-kb gene cluster for the utilization of arabinan, a branched polysaccharide that is part of the plant cell wall. the bacterium encodes a unique three-component regulatory system (arapst) that includes a sugar-binding lipoprotein (arap), a histidine sensor kinase (aras), and a response regulator (arat) and lies adjacent to an atp-binding cassette (abc) arabinose transport system (araegh). the lipoprotein (arap) speci ... | 2011 | 21460081 |
| biosynthesis of ω-alicyclic fatty acids induced by cyclic precursors and change of membrane fluidity in thermophilic bacteria geobacillus stearothermophilus and meiothermus ruber. | two thermophilic strains belonging to geobacillus stearothermophilus and meiothermus ruber, which naturally do not synthesize ω-alicyclic fatty acids (ω-fas) were cultivated with cyclopropyl, cyclobutyl, cyclopentyl and cyclohexyl carboxylic acids. gas chromatography-mass spectrometry analysis of fatty acid methyl and picolinyl esters showed that both strains are able to synthesize ω-fas when cultivated with the appropriate precursor. the incorporation of cyclic acids influenced the whole fa com ... | 2011 | 21487936 |
| solution structure of rnase p rna. | the ribonucleoprotein enzyme ribonuclease p (rnase p) processes trnas by cleavage of precursor-trnas. rnase p is a ribozyme: the rna component catalyzes trna maturation in vitro without proteins. remarkable features of rnase p include multiple turnovers in vivo and ability to process diverse substrates. structures of the bacterial rnase p, including full-length rnas and a ternary complex with substrate, have been determined by x-ray crystallography. however, crystal structures of free rna are si ... | 2011 | 21531920 |
| isolation and characterization of aerobic thermophilic bacteria from the savusavu hot springs in fiji. | the relative isolation and unique physical properties of the savusavu hot springs in fiji may yield unique thermophiles. this study was conducted to determine the presence of aerobic thermophilic bacteria in these hot springs. a total of 104 thermophilic bacterial isolates were characterized and using thermus and bacillus strains as controls, 58% of the isolates were identified as anoxybacillus flavithermus, 19% as geobacillus stearothermophilus/bacillus licheniformis, 10% as thermus sp. tg153 a ... | 2008 | 21558730 |
| receptor-transporter interactions of canonical atp-binding cassette import systems in prokaryotes. | atp-binding cassette (abc) transport systems mediate the translocation of solutes across biological membranes at the expense of atp. they share a common modular architecture comprising two pore-forming transmembrane domains and two nucleotide binding domains. in prokaryotes, abc transporters are involved in the uptake of a large variety of chemicals, including nutrients, osmoprotectants and signal molecules. in pathogenic bacteria, some abc importers are virulence factors. canonical abc import s ... | 2011 | 21561685 |
| rapd-based screening for spore-forming bacterial populations in uruguayan commercial powdered milk. | the occurrence of spore-forming bacteria in powdered milk is of concern to the dairy industry due to potential deleterious effects including those resulting from proteolytic and lipolytic activities. twenty-two powdered milk samples representative of spring and summer production obtained from uruguayan retail stores were analyzed for type and number of thermophilic and spore-forming bacterial species. bacillus licheniformis isolates were found to be the most prominent milk powder contaminant fol ... | 2011 | 21565415 |
| crystal structure of the novel paib transcriptional regulator from geobacillus stearothermophilus. | | 2011 | 21633969 |
| the role of calcium ions in the stability and instability of a thermolysin-like protease. | thermolysin and other secreted broad-specificity proteases such as subtilisin or alpha-lytic protease, are produced as pre-pro-proteins that stay at least partially unfolded while in the cytosol. after secretion, the pro-proteases fold to their active conformations in a process that includes the autolytic removal of the pro-peptide. we review the life cycle of the thermolysin-like protease from bacillus stearothermophilus in light of the calcium dependent stability and instability of the n-termi ... | 2011 | 21648000 |
| identification of the substrate binding site in the n-terminal tbp-like domain of rnase h3. | ribonuclease h3 from bacillus stearothermophilus (bst-rnase h3) has the n-terminal tbp-like substrate-binding domain. to identify the substrate binding site in this domain, the mutant proteins of the intact protein and isolated n-domain, in which six of the seventeen residues corresponding to those involved in dna binding of tbp are individually mutated to ala, were constructed. all of them exhibited decreased enzymatic activities and/or substrate-binding affinities when compared to those of the ... | 2011 | 21664908 |
| urine antibiotic activity in patients presenting to hospitals in laos: implications for worsening antibiotic resistance. | abstract. widespread use of antibiotics may be important in the spread of antimicrobial resistance. we estimated the proportion of lao in- and outpatients who had taken antibiotics before medical consultation by detecting antibiotic activity in their urine added to lawns of bacillus stearothermophilus, escherichia coli, and streptococcus pyogenes. in the retrospective (n = 2,058) and prospective studies (n = 1,153), 49.7% (95% confidence interval [ci] = 47.4-52.0) and 36.2% (95% ci = 33.4-38.9), ... | 2011 | 21813851 |
| splint sterilization-a potential registration hazard in computer-assisted surgery. | purpose: registration of preoperative targeting information for the intraoperative situation is a crucial step in computer-assisted surgical interventions. point-to-point registration using acrylic splints is among the most frequently used procedures. there are, however, no generally accepted recommendations for sterilization of the splint. an appropriate method for the thermolabile splint would be hydrogen peroxide-based plasma sterilization. this study evaluated the potential deformation of th ... | 2011 | 21813224 |
| trimer-based design of ph-responsive protein cage results in soluble disassembled structures. | limited studies have been done on the interactions between subunits of self-assembling protein cages. e2 protein cage from bacillus stearothermophilus was investigated in this work to impart ph-sensitive disassembly profile. key amino acids were identified at the intratrimer and intertrimer interfaces, and histidine residues were introduced to these key sites to probe for their influences on the e2 assembly. we found that both the intratrimer- and the intertrimer-modified mutant proteins have th ... | 2011 | 21797220 |
| stereoselective synthesis of 2,3-diamino-2,3-dideoxy-ß-d-mannopyranosyl uronates. | with the aim to find an efficient synthetic procedure for the construction of 2,3-diamino-2,3-dideoxy-ß-d-mannuronic acids, we evaluated three mannosyl donors: (s)-phenyl 4,6-di-o-acetyl-2,3-diazido mannopyranoside, (s)-phenyl 2,3-diazido-4,6-o-benzylidene mannopyranoside, and (s)-phenyl 2,3-diazido mannopyranosyl methyl uronate. the first two mannosylating agents are rather unselective or slightly a-selective in their condensation with three different acceptors. the mannuronic acid donor on the ... | 2011 | 21793528 |
| a head-to-head comparison of hydrogen peroxide vapor and aerosol room decontamination systems. | objective.ôçânew technologies have emerged in recent years for the disinfection of hospital rooms and equipment that may not be disinfected adequately using conventional methods. there are several hydrogen peroxide-based area decontamination technologies on the market, but no head-to-head studies have been performed. design.ôçâwe conducted a head-to-head in vitro comparison of a hydrogen peroxide vapor (hpv) system (bioquell) and an aerosolized hydrogen peroxide (ahp) system (sterinis). setting. ... | 2011 | 21828962 |
| the lmo0501 gene coding for a putative transcription activator protein in listeria monocytogenes promotes growth under cold, osmotic and acid stress conditions. | in listeria monocytogenes egde, the lmo0501 gene locus encodes a protein similar to the mannitol transcription regulator (mltr) protein in bacillus subtilis and bacillus stearothermophilus. in this study we investigated its functional role in l.-ámonocytogenes egde cells in view of growth under different stress conditions. increased lmo0501 gene expression at mrna level was detected in response to cold, osmotic and organic acid stress exposure. an egde +ölmo0501 mutant strain was diminished in g ... | 2011 | 21839374 |
| transformation of chenodeoxycholic acid by thermophilic geobacillus stearothermophilus. | we performed a series of experiments with geobacillus stearothermophilus, a thermophile isolated from oil-contaminated soil in the kuwaiti desert. the organism has a good potential for the transformation of a broad spectrum of organic molecules such as steroids, amino acids, and aromatic hydrocarbons. in the present study, we tested its potential for the transformation of a bile component, chenodeoxycholic acid (cdca). five transformed products, namely, cholic acid, methylcholate, methylchenodeo ... | 2011 | 21838799 |
| toward the smallest active subdomain of a tim-barrel fold: insights from a truncated +¦-amylase. | amytm is a truncated mutant of the +¦-amylase of bacillus stearothermophilus us100. it has been derived from the wild type amylase gene via a reading frame shift, following a tandem duplication of the mutant primer, associated to an adenine base deletion. amytm was composed of 720 nucleotides encoding 240 amino acid residues out of 549 of the wild type. the amytm protein was devoided of the three catalytic residues but still retains catalytic activity. it is ca-independent maltotetraose producin ... | 2011 | 21741359 |
| importance of the c2, n7, and c8 positions to the mutagenic potential of 8-oxo-2'-deoxyguanosine with two a family polymerases. | 8-oxo-2'-deoxyguanosine (odg) is a prominent dna lesion produced from the reaction of 2'-deoxyguanosine (dg) with reactive oxygen species. while dg directs the insertion of only dctp during replication, odg can direct the insertion of either dctp or datp, allowing for the production of dg → dt transversions. when replicated by klenow fragment-exo (kf-exo), odg preferentially directs the incorporation of dctp over datp, thus decreasing its mutagenic potential. however, when replicated by a highly ... | 2011 | 22081979 |
| reduction of bacillus subtilis, bacillus stearothermophilus and streptococcus faecalis in meat batters by temperature-high hydrostatic pressure pasteurization. | people have a growing preference for fresh, healthy, palatable and nutritious meals and drinks. however, as food deterioration is a constant threat along the entire food chain, food preservation remains as necessary now as in the past. high pressure processing is one of the emerging technologies being studied as an alternative to the classical pasteurization and sterilization treatments of food. samples of fried minced pork meat were inoculated with strains of streptococcus faecalis and with spo ... | 2001 | 22062669 |
| Taxonomic revision of the genus Geobacillus: emendation of Geobacillus, G. stearothermophilus, G. jurassicus, G. toebii, G. thermodenitrificans and G. thermoglucosidans (nom. corrig., formerly 'thermoglucosidasius'); transfer of Bacillus thermantarcticus to the genus as G. thermantarcticus; proposal of Caldibacillus debilis gen. nov., comb. nov.; transfer of G. tepidamans to Anoxybacillus as A. tepidamans and proposal of Anoxybacillus caldiproteolyticus sp. nov. | Sixty-two strains of thermophilic aerobic endospore-forming bacteria were subjected to polyphasic taxonomic study including 16S rRNA gene sequence analysis, polar lipid and fatty acid analysis, phenotypic characterization, and DNA-DNA hybridization experiments. Distinct clusters of the species Geobacillus stearothermophilus, Geobacillus thermodenitrificans, Geobacillus toebii and Geobacillus thermoglucosidasius were formed, allowing their descriptions to be emended, and the distinctnesses of the ... | 2011 | 21856988 |
| How to change the oligomeric state of a circular protein assembly: switch from 11-subunit to 12-subunit TRAP suggests a general mechanism. | Many critical cellular functions are performed by multisubunit circular protein oligomers whose internal geometry has evolved to meet functional requirements. The subunit number is arguably the most critical parameter of a circular protein assembly, affecting the internal and external diameters of the assembly and often impacting on the protein's function. Although accurate structural information has been obtained for several circular proteins, a lack of accurate information on alternative oligo ... | 2011 | 21984911 |
| Gas discharge plasmas are effective in inactivating Bacillus and Clostridium spores. | Bacterial spores are the most resistant form of life and have been a major threat to public health and food safety. Nonthermal atmospheric gas discharge plasma is a novel sterilization method that leaves no chemical residue. In our study, a helium radio-frequency cold plasma jet was used to examine its sporicidal effect on selected strains of Bacillus and Clostridium. The species tested included Bacillus subtilis, Bacillus stearothermophilus, Clostridium sporogenes, Clostridium perfringens, Clos ... | 2011 | 22075631 |
| novel thermostable and alkalitolerant amylase production by geobacillus stearothermophilus hp 3. | this study reports the production and characterisation of a novel thermostable alkaline amylase from the newly isolated geobacillus stearothermophilus hp 3. the optimum temperature and ph for enzyme production was 55°c and 9.0, respectively. the amylase powder obtained from the culture filtrate by pre-chilled acetone treatment was stable over a wide ph range and liquefied thick starch slurries at 75°c. ca(2+) and co(2+) were required for the thermostability of the enzyme preparation. the present ... | 2011 | 22122512 |
| Gating NO Release from Nitric Oxide Synthase. | We have investigated the kinetics of NO escape from Geobacillus stearothermophilus nitric oxide synthase (gsNOS). Previous work indicated that NO release was gated at position 223 in mammalian enzymes; our kinetics experiments include mutants at that position along with measurements on the wild type enzyme. Employing stopped-flow UV-vis methods, reactions were triggered by mixing a reduced enzyme/N-hydroxy-l-arginine complex with an aerated buffer solution. NO release kinetics were obtained for ... | 2011 | 22148177 |
| biochemical properties of recombinant leucine aminopeptidase ii from bacillus stearothermophilus and potential applications in the hydrolysis of chinese anchovy (engraulis japonicus) proteins. | the effects of various factors on the activity and conformation of recombinant leucine aminopeptidase ii (rlap ii) from bacillus stearothermophilus and its potential utilization in the hydrolysis of anchovy proteins were investigated. the optimal temperature and ph of rlap ii were 55 °c and 8.0 in phosphate buffer, and its activity was strongly stimulated by co(2+). conformational studies indicated that maintaining the α-helical structure had a critical effect on rlap ii activity. rlap ii was us ... | 2011 | 22148180 |
| creation of metal-independent hyperthermophilic l-arabinose isomerase by homologous recombination. | hyperthermophilic l-arabinose isomerases (ais) are useful in the commercial production of d-tagatose as a low-calorie bulk sweetener. their catalysis and thermostability are highly dependent on metals, which is a major drawback in food applications. to study the role of metal ions in the thermostability and catalysis of hyperthermophilic ai, four enzyme chimeras were generated by pcr-based hybridization to replace the variable n- and c-terminal regions of hyperthermophilic thermotoga maritima ... | 2011 | 22103589 |
| Development of a fluorescently labeled thermostable DHFR for studying conformational changes associated with inhibitor binding. | A fluorescently-labeled, conformationally-sensitive Bacillus stearothermophilus (Bs) dihydrofolate reductase (DHFR) (C73A/S131C(MDCC) DHFR) was developed and used to investigate kinetics and protein conformational motions associated with methotrexate (MTX) binding. This construct bears a covalently-attached fluorophore, N-[2-(1-maleimidyl)ethyl]-7-(diethylamino)coumarin-3-carboxamide (MDCC) attached at a distal cysteine, introduced by mutagenesis. The probe is sensitive to the local molecular en ... | 2011 | 21907182 |
| Effect of Bacillus subtilis BsuM restriction-modification on plasmid transfer by polyethylene glycol-induced protoplast fusion. | Polyethylene glycol (PEG)-induced cell fusion is a promising method to transfer larger DNA from one cell to another than conventional genetic DNA transfer systems. The laboratory strain Bacillus subtilis 168 contains a restriction (R) and modification (M) system, BsuM, which recognizes the sequence 5'-CTCGAG-3'. To study whether the BsuM system affects DNA transfer by the PEG-induced cell fusion between R(+)M(+) and R(-)M(-) strains, we examined transfer of plasmids pHV33 and pLS32neo carrying n ... | 2011 | 22092861 |
| Meticillin-resistant Staphylococcus aureus is more resistant to vaporized hydrogen peroxide than commercial Geobacillus stearothermophilus biological indicators. | Vaporized hydrogen peroxide (VHP) is increasingly used in the decontamination of hospital isolation rooms. Commercially available bioindicators, most frequently Geobacillus stearothermophilus spores, are used to assess the efficacy of the decontamination phase. Staphylococcus aureus, including meticillin-resistant S. aureus (MRSA), produce catalase, which breaks down VHP, therefore potentially making it resistant to the decontamination phase. | 2012 | 22137066 |
| relevant factors affecting microbial surface decontamination by pulsed light. | pulsed light (pl) uses intense flashes of white light rich in ultraviolet (uv) light for decontamination. a log-reduction higher than 5 was obtained in one flash and at fluences lower than 1.8j/cm(2) on spores of a range of spore-forming bacteria, of vegetative cells of non-spore-forming bacteria and on yeasts spread on agar media. vegetative cells were more sensitive than spores. the inactivation by pl of bacillus subtilis, b. atrophaeus, b. cereus, geobacillus stearothermophilus, and aspergill ... | 2012 | 21924512 |
| The effect of lipids on the enzymatic activity of 6-phosphofructo-1-kinase from B. stearothermophilus. | 6-Phosphofructo-1-kinase (PFK-1), a major regulatory enzyme in the glycolysis pathway, is a cytoplasmic enzyme with complicated allosteric kinetics. Here we investigate the effects of lipids on the activity of PFK from Bacillus stearothermophilus (BsPFK), to determine whether BsPFK shares any of the membrane binding or lipid binding properties reported for some mammalian PFKs. Our results show that large unilamellar vesicles (LUVs) composed of either the phospholipid 1,2-dioleoyl-sn-glycero-3-ph ... | 2011 | 21871874 |
| suitability of different β-galactosidases as reporter enzymes in bacillus subtilis. | the suitability of three β-galactosidases as reporter enzymes for promoter expression analyses was investigated in bacillus subtilis with respect to various temperature conditions during cultivation and assay procedures. starting from the hypothesis that proteins derived from diverse habitats have different advantages as reporters at different growth temperatures, the beta-galactosidases from the thermophilic organism bacillus stearothermophilus, from the mesophilic bacterium escherichia coli an ... | 2011 | 22052389 |
| Triterpenes as a-glucosidase inhibitors from Fagus hayatae. | Triterpenoids, 1-3, 8 and 9, along with 24 known compounds were isolated from leaves and twigs of Fagus hayatae. Of these, compound 1, 1,10-seco-3ß,10a,23-trihydroxyolean-12-ene-1,28-dioic acid 1,23-lactone, possesses a hitherto unknown 1,10-seco-oleanane skeleton. In addition, 2,3-seco-20(29)-lupene-2,3-dioic acid (16), previously described as a synthetic product, is now established as a plant natural product; the neolignan-9'-O-rhamnoside 19 is also characterized herein. Their structures were ... | 2011 | 22169017 |
| tryptophan oxidative metabolism catalyzed by geobacillus stearothermophilus: a thermophile isolated from kuwait soil contaminated with petroleum hydrocarbons. | tryptophan metabolism has been extensively studied in humans as well as in soil. its metabolism takes place mainly through kynurenine pathway yielding hydroxylated, deaminated and many other products of physiological significance. however, tryptophan metabolism has not been studied in an isolated thermophilic bacterium. geobacillus stearothermophilus is a local thermophile isolated from kuwait desert soil contaminated with petroleum hydrocarbons. the bacterium grows well at 65 °c in 0.05 m phosp ... | 2011 | 22084599 |
| a new family of carbohydrate esterases is represented by a gdsl hydrolase/acetylxylan esterase from geobacillus stearothermophilus. | acetylxylan esterases hydrolyze the ester linkages of acetyl groups at positions 2 and/or 3 of the xylose moieties in xylan and play an important role in enhancing the accessibility of xylanases to the xylan backbone. the hemicellulolytic system of the thermophilic bacterium geobacillus stearothermophilus t-6 comprises a putative acetylxylan esterase gene, axe2. the gene product belongs to the gdsl hydrolase family and does not share sequence homology with any of the carbohydrate esterases in th ... | 2011 | 21994937 |
| structure of the apo form of bacillus stearothermophilus phosphofructokinase. | the crystal structure of the unliganded form of bacillus stearothermophilus phosphofructokinase (bspfk) was determined using molecular replacement to 2.8 å resolution (protein data bank entry 3u39 ). the apo bspfk structure serves as the basis for the interpretation of any structural changes seen in the binary or ternary complexes. when the apo bspfk structure is compared with the previously published liganded structures of bspfk, the structural impact that the binding of the ligands produces i ... | 2012 | 22212099 |
| crystal structure of quinol-dependent nitric oxide reductase from geobacillus stearothermophilus. | the structure of quinol-dependent nitric oxide reductase (qnor) from g. stearothermophilus, which catalyzes the reduction of no to produce the major ozone-depleting gas n(2)o, has been characterized at 2.5 å resolution. the overall fold of qnor is similar to that of cytochrome c-dependent nor (cnor), and some structural features that are characteristic of cnor, such as the calcium binding site and hydrophilic cytochrome c domain, are observed in qnor, even though it harbors no heme c. in contras ... | 2012 | 22266822 |
| extraction of copper from malanjkhand low-grade ore by bacillus stearothermophilus. | thermophilic bacteria are actively prevalent in hot water springs. their potential to grow and sustain at higher temperatures makes them exceptional compare to other microorganism. the present study was initiated to isolate, identify and determine the feasibility of extraction of copper using thermophilic heterotrophic bacterial strain. bacillus stearothermophilus is a thermophilic heterotrophic bacterium isolated from hot water spring, atri, orissa, india. this bacterium was adapted to low-grad ... | 2011 | 23024410 |
| role for cis-acting rna sequences in the temperature-dependent expression of the multiadhesive lig proteins in leptospira interrogans. | the spirochete leptospira interrogans causes a systemic infection that provokes a febrile illness. the putative lipoproteins liga and ligb promote adhesion of leptospira to host proteins, interfere with coagulation, and capture complement regulators. in this study, we demonstrate that the expression level of the liga and ligb proteins was substantially higher when l. interrogans proliferated at 37°c instead of the standard culture temperature of 30°c. the rna comprising the 175-nucleotide 5' unt ... | 2013 | 24013626 |
| small-angle x-ray scattering for imaging of surface layers on intact bacteria in the native environment. | crystalline cell surface layers (s-layers) represent a natural two-dimensional (2d) protein self-assembly system with nanometer-scale periodicity that decorate many prokaryotic cells. here, we analyze the s-layer on intact bacterial cells of the gram-positive organism geobacillus stearothermophilus atcc 12980 and the gram-negative organism aquaspirillum serpens mw5 by small-angle x-ray scattering (saxs) and relate it to the structure obtained by transmission electron microscopy (tem) after plati ... | 2013 | 23504021 |
| multiple regulatory mechanisms control the expression of the geobacillus stearothermophilus gene for extracellular xylanase. | geobacillus stearothermophilus t-6 produces a single extracellular xylanase (xyn10a) capable of producing short, decorated xylo-oligosaccharides from the naturally branched polysaccharide, xylan. gel retardation assays indicated that the master negative regulator, xylr, binds specifically to xylr operators in the promoters of xylose and xylan-utilization genes. this binding is efficiently prevented in vitro by xylose, the most likely molecular inducer. expression of the extracellular xylanase is ... | 2014 | 25070894 |
| kinetics of co recombination to the heme in geobacillus stearothermophilus nitric oxide synthase. | we report the kinetics of co rebinding to the heme in his134ser, ile223val and his134ser/ile223ser mutants of geobacillus stearothermophilus nitric oxide synthase (gsnos). the amplitudes of the two observed kinetics phases, which are insensitive to co concentration, depend on enzyme concentration. we suggest that two forms of gsnos are in equilibrium under the conditions employed (6.1-27 µm gsnos with 20 or 100% co atmosphere). the kinetics of co rebinding to the heme do not depend on the identi ... | 2012 | 23976816 |
| regulation of the thermoalkaliphilic f1-atpase from caldalkalibacillus thermarum. | the crystal structure has been determined of the f1-catalytic domain of the f-atpase from caldalkalibacillus thermarum, which hydrolyzes adenosine triphosphate (atp) poorly. it is very similar to those of active mitochondrial and bacterial f1-atpases. in the f-atpase from geobacillus stearothermophilus, conformational changes in the ε-subunit are influenced by intracellular atp concentration and membrane potential. when atp is plentiful, the ε-subunit assumes a "down" state, with an atp molecule ... | 2016 | 27621435 |
| allosteric regulation in phosphofructokinase from the extreme thermophile thermus thermophilus. | an investigation into the kinetics and regulatory properties of the type-1 phosphofructokinase (pfk) from the extreme thermophile thermus thermophilus (ttpfk) reveals an enzyme that is inhibited by pep and activated by adp by modifying the affinity exhibited for the substrate fructose 6-phosphate (fru-6-p) in a manner analogous to other prokaryotic pfks. however, ttpfk binds both of these allosteric ligands significantly more tightly than other bacterial pfks while effecting a substantially more ... | 2013 | 24328040 |
| biophysical characterization of a recombinant aminopeptidase ii from the thermophilic bacterium bacillus stearothermophilus. | in the present study, the biophysical properties of his6-tagged bacillus stearothermophilus aminopeptidase ii (his6-tagged bsampii) are characterized in detail by gel-filtration, analytical ultracentrifugation, and various spectroscopic techniques. using size-exclusion chromatography and analytical ultracentrifugation, we demonstrate that his6-tagged bsampii exists predominantly as a dimer in solution. the enzyme is active and stable at phs ranging from 6.5 to 8.5. far-uv circular dichroism anal ... | 2013 | 24165863 |
| crystallization and preliminary crystallographic analysis of ganb, a gh42 intracellular β-galactosidase from geobacillus stearothermophilus. | geobacillus stearothermophilus t-6 is a gram-positive thermophilic soil bacterium that contains a multi-enzyme system for the utilization of plant cell-wall polysaccharides, including xylan, arabinan and galactan. the bacterium uses a number of endo-acting extracellular enzymes that break down the high-molecular-weight polysaccharides into decorated oligosaccharides. these oligosaccharides enter the cell and are further hydrolyzed into sugar monomers by a set of intracellular glycoside hydrolase ... | 2013 | 24100561 |
| enhancing allosteric inhibition in thermus thermophilus phosphofructokinase. | the coupling between the binding of the substrate fru-6-p and the inhibitor phospho(enol)pyruvate (pep) in phosphofructokinase (pfk) from the extreme thermophile thermus thermophilus is much weaker than that seen in a pfk from bacillus stearothermophilus. from the crystal structures of bacillus stearothermophilus pfk (bspfk) the residues at positions 59, 158, and 215 in bspfk are located on the path leading from the allosteric site to the nearest active site and are part of the intricate hydroge ... | 2014 | 25531642 |
| concentric-flow electrokinetic injector enables serial crystallography of ribosome and photosystem ii. | we describe a concentric-flow electrokinetic injector for efficiently delivering microcrystals for serial femtosecond x-ray crystallography analysis that enables studies of challenging biological systems in their unadulterated mother liquor. we used the injector to analyze microcrystals of geobacillus stearothermophilus thermolysin (2.2-å structure), thermosynechococcus elongatus photosystem ii (<3-å diffraction) and thermus thermophilus small ribosomal subunit bound to the antibiotic paromomyci ... | 2015 | 26619013 |
| specific internalisation of gold nanoparticles into engineered porous protein cages via affinity binding. | porous protein cages are supramolecular protein self-assemblies presenting pores that allow the access of surrounding molecules and ions into their core in order to store and transport them in biological environments. protein cages' pores are attractive channels for the internalisation of inorganic nanoparticles and an alternative for the preparation of hybrid bioinspired nanoparticles. however, strategies based on nanoparticle transport through the pores are largely unexplored, due to the diffi ... | 2016 | 27622533 |
| cold atmospheric air plasma sterilization against spores and other microorganisms of clinical interest. | physical cold atmospheric surface microdischarge (smd) plasma operating in ambient air has promising properties for the sterilization of sensitive medical devices where conventional methods are not applicable. furthermore, smd plasma could revolutionize the field of disinfection at health care facilities. the antimicrobial effects on gram-negative and gram-positive bacteria of clinical relevance, as well as the fungus candida albicans, were tested. thirty seconds of plasma treatment led to a 4 t ... | 2012 | 22582068 |
| a novel alkaliphilic bacillus esterase belongs to the 13(th) bacterial lipolytic enzyme family. | microbial derived lipolytic hydrolysts are an important class of biocatalysts because of their huge abundance and ability to display bioactivities under extreme conditions. in spite of recent advances, our understanding of these enzymes remains rudimentary. the aim of our research is to advance our understanding by seeking for more unusual lipid hydrolysts and revealing their molecular structure and bioactivities. | 2013 | 23577139 |
| the cyclic antibacterial peptide enterocin as-48: isolation, mode of action, and possible food applications. | enterocin as-48 is a circular bacteriocin produced by enterococcus. it contains a 70 amino acid-residue chain circularized by a head-to-tail peptide bond. the conformation of enterocin as-48 is arranged into five alpha-helices with a compact globular structure. enterocin as-48 has a wide inhibitory spectrum on gram-positive bacteria. sensitivity of gram-negative bacteria increases in combination with outer-membrane permeabilizing treatments. eukaryotic cells are bacteriocin-resistant. this catio ... | 2014 | 25493478 |
| cyclic lipodepsipeptides produced by pseudomonas spp. naturally present in raw milk induce inhibitory effects on microbiological inhibitor assays for antibiotic residue screening. | two pseudomonas strains, identified as closely related to pseudomonas tolaasii, were isolated from milk of a farm with frequent false-positive delvotest results for screening putative antibiotic residues in raw milk executed as part of the regulatory quality programme. growth at 5 to 7°c of these isolates in milk resulted in high lipolysis and the production of bacterial inhibitors. the two main bacterial inhibitors have a molecular weight of 1168.7 and 1140.7 da respectively, are heat-tolerant ... | 2014 | 24853676 |
| novel antigen delivery systems. | vaccines represent the most relevant contribution of immunology to human health. however, despite the remarkable success achieved in the past years, many vaccines are still missing in order to fight important human pathologies and to prevent emerging and re-emerging diseases. for these pathogens the known strategies for making vaccines have been unsuccessful and thus, new avenues should be investigated to overcome the failure of clinical trials and other important issues including safety concern ... | 0 | 26279977 |
| selective inhibition of bacterial tryptophanyl-trna synthetases by indolmycin is mechanism-based. | indolmycin is a natural tryptophan analog that competes with tryptophan for binding to tryptophanyl-trna synthetase (trprs) enzymes. bacterial and eukaryotic cytosolic trprss have comparable affinities for tryptophan (km ∼ 2 μm), and yet only bacterial trprss are inhibited by indolmycin. despite the similarity between these ligands, bacillus stearothermophilus (bs)trprs preferentially binds indolmycin ∼1500-fold more tightly than its tryptophan substrate. kinetic characterization and crystallogr ... | 2015 | 26555258 |
| the n-terminal fragment of acanthamoeba polyphaga mimivirus tyrosyl-trna synthetase (tyrrs(apm)) is a monomer in solution. | acanthamoeba polyphaga mimivirus tyrosyl-trna synthetase (tyrrsapm) was the first reported aminoacyl-trna synthetase of viral origin. the previous crystal structure of tyrrsapm showed a non-canonical orientation of the dimer conformation and the cp1 domain, responsible for dimer formation, displays a major modification of a motif structurally conserved in other tyrrs structures. an earlier study reported that bacillus stearothermophilus n-terminal tyrrs exists as a dimer under native conditions. ... | 2013 | 23384724 |
| efficacy, efficiency and safety aspects of hydrogen peroxide vapour and aerosolized hydrogen peroxide room disinfection systems. | this was a head-to-head comparison of two hydrogen-peroxide-based room decontamination systems. | 2012 | 22306442 |
| isolation of an indigenous imidacloprid-degrading bacterium and imidacloprid bioremediation under simulated in situ and ex situ conditions. | the bacterial community structure and its complexity of the enrichment culture during the isolation and screening of imidacloprid-degrading strain were studied using denaturating gradient gel electrophoresis analysis. the dominant bacteria in the original tea rhizosphere soil were uncultured bacteria, rhizobium sp., sinorhizobium, ochrobactrum sp., alcaligenes, bacillus sp., bacterium, klebsiella sp., and ensifer adhaerens. the bacterial community structure was altered extensively and its comple ... | 2013 | 23985542 |
| expression, purification and characterization of a feruloyl esterase a from aspergillus flavus. | feruloyl esterases are key enzymes involved in the complete hydrolysis of hemicellulose. in the present study, the encoding sequence of putative feruloyl esterase a (affaea) was cloned from genomic dna from aspergillus flavus and expressed in pichia pastoris. the purified recombinant affaea had apparent relative molecular mass of about 40,000 and had an optimum ph of 6.0, although it was stable at ph values ranging from 4.5 to 8.0. the optimum temperature for affaea was 58°c. affaea had hydrolyt ... | 2013 | 23981381 |
| α-glucosylated 6-gingerol: chemoenzymatic synthesis using α-glucosidase from halomonas sp. h11, and its physical properties. | 6-gingerol [(s)-5-hydroxy-1-(4-hydroxy-3-methoxyphenyl)decan-3-one] is a biologically active compound and is abundant in the rhizomes of ginger (zingiber officinale). it has some beneficial functions in healthcare, but its use is limited because of its insolubility in water and its heat-instability. to improve these physical properties, the glucosylation of 6-gingerol was investigated using α-glucosidases (ec. 3.2.1.20) from aspergillus niger, aspergillus nidulans abpu1, acremonium strictum, hal ... | 2012 | 22537860 |
| decontamination of sugar syrup by pulsed light. | the pulsed light produced by xenon flash lamps was applied to 65 to 67 °brix sugar syrups artificially contaminated with suspensions of saccharomyces cerevisiae and with spores of bacillus subtilis, geobacillus stearothermophilus, alicyclobacillus acidoterrestris, and aspergillus niger. the emitted pulsed light contained 18.5 % uv radiation. at least 3-log reductions of s. cerevisiae, b. subtilis, g. stearothermophilus, and a. acidoterrestris suspended in 3-mm-deep volumes of sugar syrup were ob ... | 2012 | 22564941 |
| a novel organic solvent- and detergent-stable serine alkaline protease from trametes cingulata strain ctm10101. | a protease-producing fungus was isolated from an alkaline wastewater of chemical industries and identified as trametes cingulata strain ctm10101 on the basis of the its rdna gene-sequencing. it was observed that the fungus strongly produce extracellular protease grown at 30°c in potato-dextrose-broth (pdb) optimized media (13500u/ml). the pure serine protease isolated by trametes cingulata (designated sptc) was purified by ammonium sulfate precipitation-dialysis followed by heat-treatment and un ... | 2016 | 27296442 |
| phylogenetic and comparative sequence analysis of thermostable alpha amylases of kingdom archea, prokaryotes and eukaryotes. | alpha amylase family is generally defined as a group of enzymes that can hydrolyse and transglycosylase α-(1, 4) or α-(1, 6) glycosidic bonds along with the preservation of anomeric configuration. for the comparative analysis of alpha amylase family, nucleotide sequences of seven thermo stable organisms of kingdom archea i.e. pyrococcus furiosus (100-105°c), kingdom prokaryotes i.e. bacillus licheniformis (90-95°c), geobacillus stearothermophilus (75°c), bacillus amyloliquefaciens (72°c), bacill ... | 2014 | 25187685 |
| spore-forming bacteria responsible for food spoilage. | this review explores the main spore-forming bacteria involved in the spoilage of various processed foods. bakery products are specifically spoiled by bacillus species, the dominant one being bacillus amyloliquefaciens, while different clostridium species classically contaminate refrigerated vacuum-packed meats. these two genera have also been isolated from milk products, even when milk is pasteurized, sterilized, dehydrated or fermented, according to heat treatment and storage temperature. final ... | 2016 | 27989764 |
| high-pressure thermal sterilization: food safety and food quality of baby food puree. | the benefits that high-pressure thermal sterilization offers as an emerging technology could be used to produce a better overall food quality. due to shorter dwell times and lower thermal load applied to the product in comparison to the thermal retorting, lower numbers and quantities of unwanted food processing contaminants (fpcs), for example, furan, acrylamide, hmf, and mcpd-esters could be formed. two spore strains were used to test the technique; geobacillus stearothermophilus and bacillus a ... | 2014 | 24547697 |