| impact of heavy smoking on the clinical, microbiological and immunological parameters of patients with dental implants: a prospective cross-sectional study. | the aim of the present study was to investigate how heavy smoking influences the clinical, microbiological, and host-response characteristics in peri-implant sulcus fluid of patients with healthy dental implants. | 2016 | 26171870 |
| treponema denticola, porphyromonas gingivalis, and tannerella forsythia induce cell death and release of endogenous danger signals. | the aim of this study was to analyze whether periodontopathogens induced inflammatory cell death and the release of diverse endogenous danger molecules in thp-1-derived macrophages. | 2017 | 27697692 |
| rapid identification and quantitation for oral bacteria based on short-end capillary electrophoresis. | high-speed capillary electrophoresis (hsce) is a promising technology applied in ultra-rapid and high-performance analysis of biomolecules (such as nucleic acids, protein). in present study, the short-end capillary electrophoresis coupled with one novel space domain internal standard method (sdis) was employed for the rapid and simultaneous analysis of specific genes from three oral bacteria (porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f)). the reliabilit ... | 2016 | 27591633 |
| periodontitis contributes to adipose tissue inflammation through the nf-<kappa>b, jnk and erk pathways to promote insulin resistance in a rat model. | this study aimed to investigate the mechanism by which periodontitis affects the inflammatory response and systemic insulin resistance in the white adipose and liver tissues in an obese rat model. the obese model was generated by feeding rats a high fat diet. the periodontitis model was induced by ligatures and injection of "red complex", which consisted of porphyromonas gingivalis, treponema denticola, and tannerella forsythia, for two weeks. when compared with rats without periodontitis, fasti ... | 2016 | 27565999 |
| persistence of porphyromonas gingivalis is a negative predictor in patients with moderate to severe periodontitis after nonsurgical periodontal therapy. | the aim of this study was to evaluate the quality of prediction for stable results after nonsurgical periodontal therapy by several microbiological variables of the subgingival biofilm and biomarkers of gingival crevicular fluid or oral lavage. | 2017 | 27558382 |
| [gender-related marker pathogens of periodontal disease in chronic periodontitis]. | by using qpcr system, women as well as men were found to have an equal periodontal pathogen colonization. however, the women are subjected to have a higher risk of chronic periodontitis onsets. women with the chronic periodontitis usually expose an evident hypercolonization with a single pathogen. p. gingivalis is the most prevalent causative agent of the chronic periodontitis in women but not in men. in health as well as in the chronic periodontitis a complex of periodontal pathogens forms such ... | 2016 | 27367192 |
| the influences of periodontal status and periodontal pathogen quantity on salivary 8-hydroxydeoxyguanosine and interleukin-17 levels. | periodontitis is a biofilm-initiated disease that is characterized by elevated inflammatory status. 8-hydroxydeoxyguanosine (8-ohdg) and interleukin (il)-17 are highly associated with inflammation and bone resorption and therefore are regarded as potential biomarkers for periodontitis. in this study, the associations between salivary 8-ohdg and il-17 levels and clinical and microbial parameters before and after non-surgical treatment are investigated. | 2016 | 26654345 |
| bacterial interactions in pathogenic subgingival plaque. | chronic periodontitis has a polymicrobial biofilm aetiology. polymicrobial biofilms are complex, dynamic microbial communities formed by two or more bacterial species that are important for the persistence and proliferation of participating microbes in the environment. interspecies adherence, which often involves bacterial surface-associated molecules, and communications are essential in the spatial and temporal development of a polymicrobial biofilm, which in turn is necessary for the overall f ... | 2016 | 26541672 |
| polymicrobial infection alter inflammatory microrna in rat salivary glands during periodontal disease. | periodontal disease initiated by subgingival pathogens is linked with diminished secretion of saliva, and implies pathogenic bacteria dissemination to or affects secondary sites such as the salivary glands. micrornas activated in response to bacteria may modulate immune responses against pathogens. therefore, sprague-dawley rats were infected by oral lavage consisting of polymicrobial inocula, namely porphyromonas gingivalis, tannerella forsythia, and treponema denticola, or sham-infected for 12 ... | 2016 | 26481834 |
| oral microbiome in chinese patients with aggressive periodontitis and their family members. | to investigate the microbiome composition in chinese patients with aggressive periodontitis (agp), and to compare the similarity of bacterial profiles between agp patients and their family members. | 2015 | 26412568 |
| periodontal status and pathogenic bacteria after gastric bypass: a cohort study. | the aim this study was to evaluate the influence of gastric bypass surgery (gbs) on periodontal disease and quantify the periodontopathogenic bacteria in patients undergoing this surgery. | 2015 | 25924695 |
| β2-glycoprotein i-dependent anti-cardiolipin antibodies associated with periodontitis in patients with systemic lupus erythematosus. | it was reported that patients with systemic lupus erythematosus (sle) exhibited increased levels of anticardiolipin (anti-cl) antibodies, a class of antiphospholipid antibodies associated with thrombosis. β2-glycoprotein i (β2gpi) has been considered as the actual target antigen for anti-cl antibodies. this study investigates the association of periodontal infection with anti-cl antibodies in patients with sle. | 2015 | 25817824 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25507669 |
| electrophoresis of periodontal pathogens in poly(ethyleneoxide) solutions with uncoated capillary. | periodontitis is a prevalent inflammatory disease caused by different species of anaerobic bacteria such as porphyromonas gingivalis (p.g), treponema denticola (t.d), and tannerella forsythia (t.f). we compared the separation result of dna ladders in hydroxyethyl cellulose, poly(ethyleneoxide) (peo), and polyethylene glycol and analyzed the effect of polymer concentration, electric field, and temperature of the background electrolyte on the separation performance. results demonstrated that there ... | 2015 | 25447490 |
| subgingival microbial profile and production of proinflammatory cytokines in chronic periodontitis. | this review examines literature data concerning the bacterial findings in chronic periodontitis depending on pocket depth, and presents the latest published information on the presence of proinflammatory factors in periodontal environment. it has been found that chronic periodontitis affects as much as 80% of the middle-aged population; by comparison, the prevalence of aggressive periodontitis reaches up to 1-1.5%. it is accepted that this social disease is multifactorial in etiology, but the ev ... | 2014 | 25434071 |
| association between maternal periodontitis and preterm and/or low birth weight infants in normal pregnancies. | to relate the periodontal condition with the presence of periodontal bacteria in pregnant that had babies with preterm delivery or/and low weight at birth (ptlbw). | 2016 | 25394610 |
| occurrence of socransky red complex in pregnant women with and without periodontal disease. | to verify the presence of socransky red complex (porphyromonas gingivalis, treponema denticola and tannerella forsythia) and p. intermedia using polymerase chain reaction (pcr) in periodontally healthy pregnant women and pregnant women with periodontal disease, as well as its relation to arterial blood pressure and capillary glycaemia. | 2015 | 25386627 |
| the effect of conventional mechanical periodontal treatment on red complex microorganisms and clinical parameters in down syndrome periodontitis patients: a pilot study. | periodontal disease (pd) is induced by a complex microbiota, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola (together called the red complex), which triggers intense inflammatory reaction. down syndrome (ds) individuals demonstrate a high prevalence of pd compared with those who are otherwise chromosomally normal (euploids). this pilot study aimed to evaluate the effect of non-surgical periodontal treatment in ds chronic periodontitis patients on clinical and mic ... | 2015 | 25367612 |
| screening and development of dna aptamers specific to several oral pathogens. | aptamers are composed of single-stranded oilgonucleotides that can selectively bind desired molecules. it has been reported that rna or dna could act as not only a genetic messenger but also a catalyst in metabolic pathways. rna aptamers (average sizes 40-50 bp) are smaller than antibodies and have strong binding capacities to target molecules, similar to antigen-antibody interactions. once an aptamer was selected, it can be readily produced in large quantities at low cost. the objectives of thi ... | 2015 | 25269812 |
| [comparison of the effects of ginkgo biloba extract and minocycline hydrochlovide on periodontitis]. | to compare the clinical effect of ginkgo biloba extract gel (ginkgo biloba extract,egb) and minocycline hydrochloride (periocline) on periodontitis and their inhibition on putative periodontal pathogens. | 2014 | 25199931 |
| periodontal bacteria in human carotid atherothrombosis as a potential trigger for neutrophil activation. | epidemiological, biological and clinical links between periodontal and cardiovascular diseases are now well established. several human studies have detected bacterial dna corresponding to periodontal pathogens in cardiovascular samples. intraplaque hemorrhage has been associated with a higher risk of atherosclerotic plaque rupture, potentially mediated by neutrophil activation. in this study, we hypothesized that plaque composition may be related to periodontal pathogens. | 2014 | 25173070 |
| pilot study on the clinical and microbiological effect of subgingival glycine powder air polishing using a cannula-like jet. | to assess the efficacy of subgingival glycine powder air polishing (gpap) during supportive periodontal therapy (spt). | 2015 | 25156125 |
| dentilisin involvement in coaggregation between treponema denticola and tannerella forsythia. | periodontitis arises from a biofilm consisting of gram-negative anaerobic rods and spirochetes. porphyromonas gingivalis, treponema denticola, and tannerella forsythia, termed the red complex, have been co-isolated with high frequency from chronic periodontitis lesions, and these microorganisms are thought to be major pathogens of the disease. coaggregation is an important strategy in the colonization of dental plaque biofilm by these bacteria. in the present study, we investigated the coaggrega ... | 2014 | 25152229 |
| quantification of periodontal pathogens cell counts by capillary electrophoresis. | gingivitis is a highly prevalent periodontal disease around the worldwide. porphyromonas gingivalis (p.g), treponema denticola (t.d) and tannerela forsythia (t.f) were considered to be three important periodontal pathogens related to gingivitis, and research shows that the counts of periodontal pathogen cells in the patients before, during, and after fixed orthodontic appliance therapy were quite different. we proposed a simple method to extract the periodontal pathogens from the periodontal poc ... | 2014 | 25145561 |
| effects of ginkgo biloba extract on periodontal pathogens and its clinical efficacy as adjuvant treatment. | to determine the effects of ginkgo biloba extract (egb) on major periodontal pathogens in subgingival plaque. | 2014 | 25098260 |
| bioinformatic investigation of the cost management strategies of five oral microbes. | some amino acids are more energetically costly to synthesize de novo, therefore many microbes have evolved to regulate the metabolic expenditure of the cell and reduce the energy burden of extracellular unrecyclable proteins. several oral bacterial species take up amino acids and peptides obtained from proteolysis of host proteins and hence do not rely only on de novo synthesis. the aim of this study was to investigate if five oral bacterial species implement cost management strategies to reduce ... | 2015 | 25052707 |
| oral inflammation, tooth loss, risk factors, and association with progression of alzheimer's disease. | periodontitis is a polymicrobial chronic inflammatory disease of tooth-supporting tissues with bacterial etiology affecting all age groups, becoming chronic in a subgroup of older individuals. periodontal pathogens porphyromonas gingivalis, tannerella forsythia, and treponema denticola are implicated in the development of a number of inflammatory pathologies at remote organ sites, including alzheimer's disease (ad). the initial inflammatory hypothesis proposed that ad hallmark proteins were the ... | 2014 | 24946875 |
| molecular analysis of oral bacteria in dental biofilm and atherosclerotic plaques of patients with vascular disease. | oral bacteria have been detected in atherosclerotic plaques at a variable frequency; however, the connection between oral health and vascular and oral bacterial profiles of patients with vascular disease is not clearly established. the aim of this study was to evaluate the presence of oral bacterial dna in the mouth and atherosclerotic plaques, in addition to assessing the patients' caries and periodontal disease history. | 2014 | 24820755 |
| periodontal disease associated with red complex bacteria in dogs. | red complex bacteria (treponema denticola, tannerella forsythia and porphyromonas gingivalis) play a major role in the aetiology of periodontal disease in humans. this study was designed to evaluate the association of such bacteria with periodontal disease in dogs. | 2014 | 24450418 |
| [relationships of five periodontal pathogens causing subgingival plaque in patients with chronic periodontitis under different periodontal conditions]. | this study aimed to assess the relationship between the distributions of haemophilus aggregatibacter (h. aggregatibacter ), porphyromonas gingivalis (p. gingivalis ), prevotella intermedia (p. intermedia), tannerella forsythensis (t. forsythensis), and treponema denticola (t. denticola) in subgingival plaque and different periodontal conditions of chronic periodontitis. | 2013 | 24298807 |
| oral hygiene reinforcement in the simplified periodontal treatment of 1 hour. | to compare the clinical and microbiological outcome of the 1-h ultrasonic debridement of chronic periodontitis patients (cpp) with and without frequent sessions of oral hygiene reinforcement. | 2014 | 24255993 |
| pathogen levels and clinical response to periodontal treatment in patients with interleukin 8 haplotypes. | the aim of this study was to investigate the effect of non-surgical treatment of periodontitis on the levels of periodontopathogens and clinical parameters in patients with different genetic backgrounds produced by polymorphisms in the interleukin ( il8) gene. thirty patients grouped according to il8 atc/ttc or agt/ttc haplotypes were submitted to non-surgical periodontal treatment. levels of porphyromonas gingivalis, tannerella forsythia, and treponema denticola were determined in 240 subgingiv ... | 2013 | 23821559 |
| [detection of periodontal pathogens from saliva of type 2 diabetic patients in urban area of beijing]. | to investigate the prevalence of periodontal pathogens from saliva of patients with type 2 diabetes mellitus (t2dm), and to characterize the association between the glucose status and periodontal pathogens in oral cavity. | 2013 | 23751528 |
| periodontopathogens levels and clinical response to periodontal therapy in individuals with the interleukin-4 haplotype associated with susceptibility to chronic periodontitis. | periodontitis is an inflammatory disease that results from an interaction between dental biofilm agents and the host immune-inflammatory response. periodontopathogenic organisms, such as porphyromonas gingivalis, tannerella forsythia, and treponema denticola, as well as the host's susceptibility, represented by the host's genetic makeup, are the key factors that influence this complex disease. recently, we identified haplotypes in the il4 gene that were associated with chronic periodontitis (cp) ... | 2013 | 23748736 |
| clinical, microbiologic, and host response characteristics in patients with peri-implant mucositis. | to analyze the clinical, microbiologic, and host response characteristics (interleukins 1β and 6) in the peri-implant sulcus fluid (pisf) of patients with healthy dental implants and with peri-implant mucositis. | 2013 | 23748323 |
| isolation of treponema and tannerella spp. from equine odontoclastic tooth resorption and hypercementosis related periodontal disease. | red complex bacteria, i.e. porphyromonas gingivalis, treponema denticola and tannerella forsythia, are involved in the onset and progression of periodontal disease in man, yet seldom inhabit the oral cavity of healthy individuals. periodontal disease is also encountered in horses, with equine odontoclastic tooth resorption and hypercementosis (eotrh) constituting a particular form of disease. however, only little is known about the oral microbiome of healthy and periodontitis-affected equids. | 2014 | 23742079 |
| determining the presence of periodontopathic virulence factors in short-term postmortem alzheimer's disease brain tissue. | the aim of this study was to establish a link between periodontal disease and alzheimer's disease (ad) with a view to identifying the major periodontal disease bacteria (treponema denticola, tannerella forsythia, and porphyromonas gingivalis) and/or bacterial components in brain tissue from 12 h postmortem delay. our request matched 10 ad cases for tissue from brains for dementia research alongside 10 non-ad age-related controls with similar or greater postmortem interval. we exposed svgp12, an ... | 2013 | 23666172 |
| association between il8 haplotypes and pathogen levels in chronic periodontitis. | chronic periodontitis (cp) is considered to be a multifactorial disease influenced by microbial and genetic factors. the aim of the present study was to investigate whether the genetic susceptibility to cp in individuals with the il8 atc/ttc haplotype is associated with subgingival levels of periodontopathogens. sixty-five individuals, grouped according to the presence (n = 28) or absence (n = 37) of the il8 haplotype, were evaluated. after clinical periodontal evaluation, each group was subdivi ... | 2013 | 23660697 |
| interleukin-10 gene promoter polymorphisms are associated with cyclosporin a-induced gingival overgrowth in renal transplant patients. | interleukin-10 (il-10) is an anti-inflammatory cytokine whose genetic polymorphisms are associated with the production of il-10 and the susceptibility to periodontal diseases. the aim of this study was to investigate the possible association of il-10 single nucleotide polymorphisms (snps) and cyclosporin a (csa)-induced gingival overgrowth (go) in renal transplant patients in a chinese population, taking into account subgingival microbiota as additional variables. | 2013 | 23643609 |
| altered phenotype and function of dendritic cells in individuals with chronic periodontitis. | to investigate the effects of periodontal bacterial lysates on maturation and function of mature monocyte-derived dendritic cells (m-mddcs) derived from individuals with chronic periodontitis (cp) or healthy periodontal tissue (hp). | 2013 | 23623310 |
| [relationship between volatile fatty acids and porphyromonas gingivalis and treponema denticola in gingival crevicular fluids of patients with aggressive periodontitis]. | short chain fatty acids (scfas), such as succinic acid, acetic acid, propionic acid, butyric acid, etc. are metabolic product of putative periodontal pathogens, which play significant roles in periodontitis. the aim of this study was to analyze the relationship between porphyromonas gingivalis (p. gingivalis), treponema denticola (t. denticola), and the concentration of scfas in gingival crevicular fluid (gcf) of patients with aggressive periodontitis (agp). | 2013 | 23411512 |
| treponema denticola improves adhesive capacities of porphyromonas gingivalis. | porphyromonas gingivalis, an important etiological agent of periodontal disease, is frequently found associated with treponema denticola, an anaerobic spirochete, in pathogenic biofilms. however, interactions between these two bacteria are not well understood at the molecular level. in this study, we seek to link the influence of t. denticola on the expression of p. gingivalis proteases with its capacities to adhere and to form biofilms. the p. gingivalis genes encoding arg-gingipain a (rgpa), l ... | 2013 | 23194417 |
| boric acid irrigation as an adjunct to mechanical periodontal therapy in patients with chronic periodontitis: a randomized clinical trial. | the purpose of this single-masked, randomized, controlled clinical trial was to evaluate the effects of boric acid irrigation as an adjunct to scaling and root planing (srp) on clinical and microbiologic parameters and compare this method with chlorhexidine irrigation and srp alone in patients with chronic periodontitis (cp). | 2013 | 23121460 |
| prediction of periodontopathic bacteria in dental plaque of periodontal healthy subjects by measurement of volatile sulfur compounds in mouth air. | the aim of this study was to determine whether measurements of volatile sulfur compounds (vscs) are useful to predict colonization of periodontopathic bacteria. for this purpose, we assessed the relationships among distributions of 4 species of periodontopathic bacteria in tongue coating and dental plaque, oral conditions including vsc concentration in mouth air, and smoking habit of periodontal healthy young subjects. | 2013 | 23107050 |
| differential proteomic analysis of a polymicrobial biofilm. | porphyromonas gingivalis, treponema denticola, and tannerella forsythia exist in a polymicrobial biofilm associated with chronic periodontitis. the aim of this study was to culture these three species as a polymicrobial biofilm and to determine proteins important for bacterial interactions. in a flow cell all three species attached and grew as a biofilm; however, after 90 h of culture p. gingivalis and t. denticola were closely associated and dominated the polymicrobial biofilm. for comparison, ... | 2012 | 22808953 |
| effects of systemic sitafloxacin on periodontal infection control in elderly patients. | to evaluate the microbiological and clinical effects of the systemic administration of sitafloxacin (stfx) on periodontal pockets in elderly patients receiving supportive periodontal therapy (spt). | 2012 | 22616908 |
| the short-term treatment effects on the microbiota at the dorsum of the tongue in intra-oral halitosis patients--a randomized clinical trial. | this study aims to assess the effects of rinsing with zinc- and chlorhexidine-containing mouth rinse with or without adjunct tongue scraping on volatile sulfur compounds (vscs) in breath air, and the microbiota at the dorsum of the tongue. | 2013 | 22573244 |
| [relationship between the preterm low birth weight infant and the periodontal pathogen bacteria in maternal saliva]. | to investigate the possible relationship between the preterm and/or low birth weight (plbw) and three anaerobic microorganisms in saliva of their mothers, including porphyromonas gingivalis (pg), tannerella forsythia (tf), treponema denticola (td). | 2012 | 22353895 |
| the relationship between turbidity of mouth-rinsed water and oral health status. | the purpose of this study was to examine the relationship between turbidity of mouth rinsed water and oral health status such as dental and periodontal conditions, oral hygiene status, flow rate of saliva and oral bacteria. | 2013 | 22339260 |
| microbiological and ammp-8 findings depending on peri-implant disease in patients undergoing supportive implant therapy. | the aim of this study was to evaluate microbiological findings and ammp-8 level of peri-implant mucositis (m) and peri-implantitis (p) in patients undergoing supportive implant therapy (sit). eighty-nine patients with 171 implants were included. the case definitions were as follows: m: ppd ≥4mm, bop; p: ppd≥4mm, bop, radiographic bone loss ≥3.5mm. samples of peri-implant sulcular fluid (pisf) were taken from all peri-implant pockets at each implant to detect periodontal pathogens using pcr and a ... | 2017 | 28274501 |
| microbiologic findings in relation to risk assessment for periodontal disease: a cross-sectional study. | in this study, an association between a computerized risk calculator and microbiologic testing is examined in patients with periodontitis. | 2016 | 26291297 |
| the sociodemographic characteristics, periodontal health status, and subgingival microbiota of patients with chronic periodontitis and type 2 diabetes mellitus: a case-control study in a chinese population. | in china, chronic periodontitis (cp) is common in patients with type 2 diabetes mellitus (t2dm). the purpose of this study is to identify the sociodemographic characteristics associated with such patients and to assess the periodontal health status and subgingival microbiota of patients with cp and t2dm (t2dmcp) in the chinese population. | 2013 | 23030242 |
| structural insights into catalysis by βc-s lyase from streptococcus anginosus. | hydrogen sulfide (h(2)s) is a causative agent of oral malodor and may play an important role in the pathogenicity of oral bacteria such as streptococcus anginosus. in this microorganism, h(2)s production is associated with βc-s lyase (lcd) encoded by lcd gene, which is a pyridoxal 5'-phosphate (plp)-dependent enzyme that catalyzes the α,β-elimination of sulfur-containing amino acids. when lcd acts on l-cysteine, h(2)s is produced along with pyruvate and ammonia. to understand the h(2)s-producing ... | 2012 | 22674431 |
| the c- terminal region of the major outer sheath protein (msp) of treponema denticola inhibits neutrophil chemotaxis. | treponema denticola is an oral spirochete strongly associated with severe periodontal disease. a prominent virulence factor, the major outer sheath protein (msp), disorients neutrophil chemotaxis by altering the cellular phosphoinositide balance, leading to impairment of downstream chemotactic events including actin rearrangement, rac1 activation and akt activation in response to chemoattractant stimulation. the specific regions of msp responsible for interactions with neutrophils remain unknown ... | 2017 | 28296262 |
| comparative analysis of motility and other properties of treponema denticola strains. | the periodontitis-associated pathogen treponema denticola is a spirochetal bacterium that swims by rotating its cell body like a corkscrew using periplasmic flagella. we compared physiologic and pathogenic properties, including motility, in four strains of t. denticola. phase-contrast microscopy showed differential motility between the strains; atcc 35404 showed the highest motility, followed by atcc 33521, and the remaining two strains (atcc 35405 and atcc 33520) showed the lowest motility. tra ... | 2017 | 27914958 |
| multilocus sequence analysis of phylogroup 1 and 2 oral treponeme strains. | more than 75 "species-level" phylotypes of spirochete bacteria belonging to the genus treponema reside within the human oral cavity. the majority of these oral treponeme phylotypes correspond to as-yet-uncultivated taxa or strains of uncertain standing in taxonomy. here, we analyze phylogenetic and taxonomic relationships between oral treponeme strains using a multilocus sequence analysis (mlsa) scheme based on the highly conserved 16s rrna, pyrh, reca, and flaa genes. we utilized this mlsa sche ... | 2017 | 27864174 |
| essential role of the linker region in the higher catalytic efficiency of a bifunctional msra-msrb fusion protein. | many bacteria, particularly pathogens, possess methionine sulfoxide reductase a (msra) and b (msrb) as a fusion form (msrab). however, it is not clear why they possess a fusion msrab form rather than the separate enzymes that exist in most organisms. in this study, we performed biochemical and kinetic analyses of msrab from treponema denticola (tdmsrab), single-domain forms (tdmsra and tdmsrb), and catalytic cys mutants (tdmsrab(c11s) and tdmsrab(c285s)). we found that the catalytic efficiency o ... | 2016 | 27551953 |
| evaluation of the efficacy of a new oral gel as an adjunct to home oral hygiene in the management of chronic periodontitis. a microbiological study using pcr analysis. | the use of chemical devices for non-surgical periodontal therapy has led to new treatment strategies aiming primarily at infection control and oral bacterial load. over the last few decades adjunctive chemical devices has been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of a new oral gel named parodongel on the red complex organisms using polymerase chain reaction (pcr) for microbiological analysis. a total of 10 patients with a dia ... | 2016 | 27469558 |
| [the molecular genetic characteristic of species content of saliva and gingival recess under periodontitis]. | the examination was carried out of samplings of 110 patients with periodontitis (observation group) and 60 patients without pathology of periodont (comparison group). the polymerase chain reaction was used to analyze samples of saliva and contents of periodontal recesses for detecting species-specific dna fragments of porphymmonas gigngivalis, streptococcus macacae, s. mutans, s. oralis, s. salivarius, s. sangis, s. sobrinus, treponema denticola. in patients with periodontitis s. mutans, s. oral ... | 2015 | 27032255 |
| treponema denticola invasion into human gingival epithelial cells. | host cell invasion is important for periodontal pathogens in evading host defenses and spreading into deeper areas of the periodontal tissue. treponema denticola has been implicated in a number of potentially pathogenic processes, including periodontal tissue penetration. here we tested the ability of t. denticola strains to invade human gingival epithelial cells (hgec). after 2 h infection, intracellular location of t. denticola cells was confirmed by confocal laser scanning microscopy (clsm). ... | 2016 | 26806000 |
| polymerase chain reaction to evaluate the efficacy of silica dioxide colloidal solutions in the treatment of chronic periodontitis: a case control study. | the objective of this study was to compare the efficacy of supportive periodontal therapy [i.e. scaling and rooth planing (srp)] alone versus a chemical silica dioxide (sio2) colloidal solution (sdcs) device used in association with srp in the treatment of chronic periodontitis in adult patients. a total of 20 patients with a diagnosis of chronic periodontitis (40 localized chronic periodontitis sites) in the age group of 35 to 55 were selected. none of these patients had previously received any ... | 2017 | 26511193 |
| comparative analysis of calcium-binding myeloid-related protein-8/14 in saliva and serum of patients with periodontitis and healthy individuals. | this study aims to investigate calcium-binding myeloid-related protein (mrp)-8/14 in the saliva and serum of individuals with periodontitis and periodontally healthy individuals for the assessment of its role in the pathogenesis and clinical diagnosis of periodontitis. | 2016 | 26447749 |
| periimplant bacteria associated with different transmucosal designs or smoking habits. | this study is to compare periimplant microbiota associated with implant transmucosal designs or smoking habits. | 2015 | 25706261 |
| [clinical and putative periodontal pathogens' features of different sites with probing depth reduction after non-surgical periodontal treatment of patients with aggressive periodontitis]. | to evaluate the differences of clinical parameters and putative periodontal pathogens in sites of different probing depth (pd) reduction after non-surgical periodontal treatment in patients with aggressive periodontitis (agp). | 2015 | 25686322 |
| association between the presence of treponema denticola and reduced levels of antiatherogenic high density lipoprotein in periodontitis. | dyslipidemia, a disorder in the levels of cholesterol or lipoproteins in the plasma, is a feature of atherosclerosis, leading to cardiovascular disease. the objective of this study was to evaluate whether tanerella forsythia and treponema denticola are related to reduced levels of antiatherogenic high density lipoprotein (hdl). | 2015 | 25328922 |
| presence of digital dermatitis treponemes on cattle and sheep hoof trimming equipment. | digital dermatitis (dd) is an infectious foot disease causing severe lameness in dairy cattle (worldwide) and sheep (uk). this study investigated whether dd treponema phylogroups can be present on equipment used to trim ruminant hooves and, therefore, consider this trimming equipment as a possible vector for the transmission of dd. equipment was tested after being used to trim dd symptomatic and asymptomatic cattle and sheep hooves, and subsequently after disinfection of equipment. after trimmin ... | 2014 | 24821857 |
| mechanisms of il-8 suppression by treponema denticola in gingival epithelial cells. | the purpose of this study was to investigate the mechanism(s) of interleukin (il)-8 suppression by treponema denticola, one of the major periodontal pathogens, in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with wild-type (wt), dentilisin-deficient (k1) or flagellin-deficient (flge) t. denticola in the presence or absence of 2% human serum for 24 h. the levels of il-8 expression were measured with real-time reverse transcription pcr and elisa. in ... | 2014 | 24296811 |
| the fate of treponema denticola within human gingival epithelial cells. | treponema denticola is one of the major pathogens associated with chronic periodontitis. bacterial invasion into gingival tissues is a critical process in the pathogenesis of periodontal disease. we recently reported that t. denticola can invade gingival epithelial cells. the aim of this study is to determine the fate of internalized t. denticola in gingival epithelial cells. immortalized human gingival epithelial hok-16b cells were infected with 5- (and 6-) carboxy-fluorescein diacetate succini ... | 2012 | 23134612 |
| structures of trans-2-enoyl-coa reductases from clostridium acetobutylicum and treponema denticola: insights into the substrate specificity and the catalytic mechanism. | ters (trans-2-enoyl-coa reductases; ec 1.3.1.44), which specifically catalyse the reduction of crotonyl-coa to butyryl-coa using nadh as cofactor, have recently been applied in the design of robust synthetic pathways to produce butan-1-ol as a biofuel. we report in the present paper the characterization of a cater (a ter homologue in clostridium acetobutylicum), the structures of cater in apo form and in complexes with nadh and nad+, and the structure of tdter (treponema denticola ter) in comple ... | 2013 | 23050861 |
| biochemical and structural characterization of the trans-enoyl-coa reductase from treponema denticola. | the production of fatty acids is an important cellular pathway for both cellular function and the development of engineered pathways for the synthesis of advanced biofuels. despite the conserved reaction chemistry of various fatty acid synthase systems, the individual isozymes that catalyze these steps are quite diverse in their structural and biochemical features and are important for controlling differences at the cellular level. one of the key steps in the fatty acid elongation cycle is the e ... | 2012 | 22906002 |
| growth curves and morphology of three treponema subtypes isolated from digital dermatitis in cattle. | digital dermatitis (dd) is an infectious claw disease of cattle that causes painful lesions, principally along the coronary band of the claws. in the us alone, the estimated economic impact of dd is estimated to be $190 million. the etiology of dd remains unclear and there is no reliable laboratory test, so dd is most often diagnosed clinically. spirochetal bacteria of the genera treponema have been implicated in dd infections following their isolation using culture techniques, serological detec ... | 2012 | 22901455 |
| identification of four treponema species in subgingival samples by nested-pcr and their correlation with clinical diagnosis. | the relationship between different species of oral treponemas and inflammation in periodontal disease progression is complex. the purpose of this study was to analyze and compare the subgingival plaque samples collected from periodontally healthy subjects and from chronic gingivitis and periodontitis patients in order to detect the presence of t. denticola, t. pectinovorum, t. socranskii and t. vincentii using nested-pcr technology. after dna extraction from the samples using qiamp dna mini kit ... | 2012 | 22838219 |
| integrin α5β1 activates the nlrp3 inflammasome by direct interaction with a bacterial surface protein. | integrins are cell-surface heterodimeric glycoproteins composed of alpha and beta subunits that mediate cell-cell, cell-extracellular matrix, and cell-pathogen interactions. in this study, we report a specific role of integrin α5β1 in nlrp3 inflammasome activation in macrophages stimulated by td92, a surface protein of the periodontopathogen, treponema denticola. the direct interaction of td92 with the cell membrane integrin α5β1 resulted in atp release and k(+) efflux, which are the main events ... | 2012 | 22608495 |
| identification of treponema denticola in subgingival samples by pcr technology and its correlation with clinical diagnosis. | treponema denticola has been associated with gingivitis and chronic periodontitis. the aim of this study was to identify treponema denticola in subgingival samples using pcr technology and to correlate it with clinical diagnosis of subjects. the study was carried out on seventy patients (20-84 years of age; mean age, 45.06 +/- 12.58) of which 22 individuals with no detectable gingivitis or periodontitis, 4 subjects with chronic gingivitis and 44 subjects with chronic periodontitis. subgingival p ... | 2012 | 22568260 |
| prevalence of periodontal pathogens among italian patients with chronic periodontitis: a retrospective study on 2992 patients. | the aim of the present study was to evaluate the prevalence of some periodontal pathogens in italian adults with chronic periodontitis. | 2017 | 28757933 |
| the association of periodontopathic bacteria levels
in saliva and tongue coating with oral malodor in periodontitis patients. | to elucidate the association oral malodor with the levels of periodontopathic bacteria in saliva and tongue coating of periodontitis patients with oral malodor. | 2017 | 28674708 |
| chronic periodontal disease, periodontal pathogen colonization, and an increased risk of precancerous gastric lesions. | the present study assessed the association between periodontal pathogen colonization and the potential risk of developing precancerous lesions of gastric cancer (plgc) in a clinical setting. | 2017 | 28671506 |
| effects of photodynamic therapy in periodontal treatment: a randomized, controlled clinical trial. | to evaluate the effects of photodynamic therapy (pdt) in the nonsurgical treatment of chronic periodontitis. | 2017 | 28667678 |
| putative periodontal pathogens in the subgingival plaque of sudanese subjects with aggressive periodontitis. | there has been limited study of the bacterial species associated with aggressive periodontitis (agp) in high-risk populations in africa. the aim of this study was to investigate and quantify the presence of four putative periodontal pathogens in the subgingival plaque of sudanese subjects with agp. a secondary aim was to investigate the effect of varying the detection threshold on the reported prevalence of the bacterial species investigated. | 2017 | 28499236 |
| risk of superinfection in peri-implantitis after systemic broad spectrum antibiotics. | peri-implant disease has developed over the last few years as a complication that is often difficult to resolve. the disease process is mainly attributed to bacterial infection. proposed combined therapies use broad-spectrum antibiotics to halt its progression. a major associated risk is the undetected development of superinfections that are difficult to eradicate. a group of healthy individuals with advanced peri-implantitis (pi) were referred for evaluation due to severe, rapidly progressive b ... | 2017 | 28854287 |
| quantification by qpcr of pathobionts in chronic periodontitis: development of predictive models of disease severity at site-specific level. | currently, there is little evidence available on the development of predictive models for the diagnosis or prognosis of chronic periodontitis based on the qpcr quantification of subgingival pathobionts. our objectives were to: (1) analyze and internally validate pathobiont-based models that could be used to distinguish different periodontal conditions at site-specific level within the same patient with chronic periodontitis; (2) develop nomograms derived from predictive models. subgingival plaqu ... | 2017 | 28848499 |
| microbiological response to periodontal therapy and multivariable prediction of clinical outcome. | this study assessed the microbiological effects of a 2-phase antimicrobial periodontal therapy and tested microbiological, clinical, and biological markers as prognostic indicators for clinical success. | 2017 | 28844191 |
| periodontal microbioma and rheumatoid arthritis: the role of porhyromonas gingivalis. | rheumatoid arthritis is a disease, which can be described as an autoimmune response after molecular mimicry caused by infective agents. the current study aims at evaluating the correlation between rhematoid arthritis (ra) and periodontal disease (pd), with special attention to the microbioma detected in the gums. thirty-four patients with rd were recruited into the current study. among rheumatic parameters, rheumatoid factor (rf), anti-citrullinated protein antibody (ccp), hla-bdr1 and das28 wer ... | 2017 | 28691459 |
| simultaneous detection of oral pathogens in subgingival plaque and prostatic fluid of men with periodontal and prostatic diseases. | chronic prostatitis (cpr) and benign prostatic hyperplasia (bph) are complex inflammatory conditions for which etiologic determinants are still poorly defined. periodontitis is caused by subgingival colonizing bacteria in the oral cavity. the causal effect of periodontal disease on prostatic inflammation has not been established. the purpose of this study is to isolate oral pathogens from expressed prostatic secretions of patients with periodontal disease and cpr or bph. | 2017 | 28548883 |
| cadmium sulphide quantum dots with tunable electronic properties by bacterial precipitation. | we present a new method to fabricate semiconducting, transition metal nanoparticles (nps) with tunable bandgap energies using engineered escherichia coli. these bacteria overexpress the treponema denticola cysteine desulfhydrase gene to facilitate precipitation of cadmium sulphide (cds) nps. analysis with transmission electron microscopy, x-ray diffraction, and x-ray photoelectron spectroscopy reveal that the bacterially precipitated nps are agglomerates of mostly quantum dots, with diameters th ... | 2016 | 28435671 |
| microbial profile comparisons of saliva, pooled and site-specific subgingival samples in periodontitis patients. | the purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. we tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens. | 2017 | 28800622 |
| functional biomarkers for chronic periodontitis and insights into the roles of prevotella nigrescens and fusobacterium nucleatum; a metatranscriptome analysis. | periodontitis is the most prevalent inflammatory disease worldwide and is caused by a dysbiotic subgingival biofilm. here we used metatranscriptomics to determine the functional shift from health to periodontitis, the response of individual species to dysbiosis and to discover biomarkers. | 2015 | 28721234 |
| effect of pranayama on ppar-γ, nf-κb expressions and red complex microorganisms in patients with chronic periodontitis - a clinical trial. | sudarshankriya pranayama is the control of breathing movements to regulate the energy flow throughout the body, which has shown to positively affect immune function, autonomic nervous system and psychologic-neuro pathways. the practice of pranayama has been proven to show several benefits such as reduction in stress levels, relieves anxiety and depression, increase in antioxidant levels, reduces insulin resistance and improves respiratory function. | 2017 | 28764300 |
| effect of azithromycin on a red complex polymicrobial biofilm. | azithromycin has recently gained popularity for the treatment of periodontal disease, despite sparse literature supporting efficiency in treating periodontal bacterial biofilms. the aim of this study was to evaluate the effect of azithromycin on biofilms comprised of porphyromonas gingivalis, treponema denticola, and tannerella forsythia in comparison to an amoxicillin and metronidazole combination. p. gingivalis w50, t. denticola atcc35405, and t. forsythia atcc43037 grown under anaerobic condi ... | 2017 | 28748041 |
| periodontal pathogens in the etiology of pancreatic cancer. | pancreatic cancer is the fourth leading cause of cancer-related deaths worldwide. chronic pancreatitis is frequently observed in patients with pancreatic cancer, and a significant relationship between orodigestive cancer-related deaths and chronic periodontitis has been detected. porphyromonas gingivalis, tannerella forsythia, and treponema denticola, collectively called the red complex, are the major pathogens responsible for chronic periodontitis and secrete peptidylarginine deiminase. anti-p. ... | 2017 | 28611978 |
| biomarkers and bacteria around implants and natural teeth in the same individuals. | this cross-sectional study assesses cytokine levels in peri-implant crevicular fluid (picf)/gingival crevicular fluid (gcf) and a selection of subgingival/submucosal plaque bacteria from clinically healthy or diseased sites in the same individuals. | 2017 | 28440740 |
| infection and apoptosis associated with inflammation in periodontitis: an immunohistologic study. | evidence of increased apoptosis is observed in periodontitis and may be associated with destruction of the periodontal tissue caused by the increased cell death, with the release of danger signals and subsequent stimulation of the proinflammatory processes. however, the exact mechanisms associated with these processes remain unclear. this study aimed to investigate the presence of the periodontal pathogen treponema denticola, apoptosis, high mobility group box 1 as a damage-associated molecular ... | 2017 | 28686335 |
| salivary, gingival crevicular fluid and serum levels of ghrelin and chemerin in patients with periodontitis and overweight. | nutrition and body weight are modifying factors for periodontitis. the purpose of this study was to quantify two molecules (ghrelin and chemerin), released in association with food intake and obesity, in periodontally healthy and diseased individuals with respect to different body mass categories. | 2017 | 28631313 |
| effect of surgical periodontal treatment associated to antimicrobial photodynamic therapy on chronic periodontitis: a randomized controlled clinical trial. | this randomized controlled clinical trial evaluated the effects of an adjunctive single application of antimicrobial photodynamic therapy (apdt) in surgical periodontal treatment (st) in patients with severe chronic periodontitis (scp). | 2017 | 28498507 |
| oral treponeme major surface protein: sequence diversity and distributions within periodontal niches. | treponema denticola and other species (phylotypes) of oral spirochetes are widely considered to play important etiological roles in periodontitis and other oral infections. the major surface protein (msp) of t. denticola is directly implicated in several pathological mechanisms. here, we have analyzed msp sequence diversity across 68 strains of oral phylogroup 1 and 2 treponemes; including reference strains of t. denticola, treponema putidum, treponema medium, 'treponema vincentii', and 'trepone ... | 2017 | 28453906 |