| sequence and expression of a wheat gene that encodes a novel protein associated with pathogen defense. | wheat (triticum aestivum) exhibits local acquired resistance to the powdery mildew pathogen erysiphe graminis f. sp. tritici. the resistant state can be induced by a preinoculation with the nonhost pathogen e. g.f. sp. hordei, the barley powdery mildew, and is accompanied by the activation of putative defense genes. here, we report the sequence of a pathogen-induced gene, wir1a, and a corresponding cdna, wir1, that encode novel defense-related proteins of 88 and 85 amino acids, respectively. ana ... | 1992 | 1477405 |
| heterogeneity of immunohistochemical staining with pulmonary surfactant protein a among fractionated alveolar macrophages which involves metabolism of pulmonary surfactant. | alveolar macrophages (am) which are separated into four fractionated subpopulations (i, ii, iii and iv), represented differential immunohistochemical staining with antibody against pulmonary surfactant protein a (sp-a). in light microscopy, the least dense am (fraction i) were intensely stained with antibody to sp-a in numerous granules of the cytoplasm, whereas the most dense cells (fraction iv) showed immuno-reactivity with the antibody in several granules distributed in the spreading and elon ... | 1992 | 1477601 |
| expression of the 4-chlorobenzoate dehalogenase genes from pseudomonas sp.-cbs3 in escherichia coli and identification of the gene translation products. | the genes encoding the 4-chlorobenzoate dehalogenase of pseudomonas sp. strain cbs3 were, in an earlier study, cloned in escherichia coli dh1 with the cosmid vector ppsa843 and then mobilized to the 4-chlorobenzoate dehalogenase minus strain pseudomonas putida kt2440. in this paper we report on the expression of 4-chlorobenzoate dehalogenase in these clones and on the polypeptide composition of the active enzyme. the dehalogenase activity in whole cells suspended in 3.2 mm 4-chlorobenzoate (30 d ... | 1992 | 1477786 |
| new hydroxybenanomicins produced by actinomadura. | a soil microorganism, actinomadura sp. mh193-16f4, produces an antifungal antibiotic benanomicin a and several related compounds. among them, benanomicin a is the best candidate as a chemotherapeutic agent in terms of antifungal activity, toxicity and water-solubility. three novel hydroxyl congeners, 3'-hydroxybenanomicin a, 7-hydroxybenanomicin a and 7-hydroxybenanomicinone have been isolated from the culture broth of the mh193-16f4 strain or its mutant. interestingly, 3'-hydroxy-benanomicin a ... | 1992 | 1478160 |
| conserved regulatory elements in the promoter region of the n-cam gene. | genomic clones containing 5'-flanking sequences, the first exon, and the entire first intron from the chicken n-cam gene were characterized by restriction mapping and dna sequencing. a > 600-bp segment that includes the first exon is very g + c-rich and contains a large proportion of cpg dinucleotides, suggesting that it represents a cpg island. sp-1 and ap-1 consensus elements are present, but no tata- or ccaat-like elements were found within 300 bp upstream of the first exon. comparison of the ... | 1992 | 1478668 |
| treatment of low density lipophorin with lipoprotein lipase: diacylglycerol content has no effect on dissociation of apolipophorin iii from low-density lipophorin. | the mechanism of the conversion of low-density lipophorin (ldlp) to high-density lipophorin (hdlp) in long-distance flight insects was investigated using a lipoprotein lipase from a bacterium, alcaligenes sp. diacylglycerol of ldlp was steadily hydrolyzed in vitro by the lipase, resulting in a 90% loss of diacylglycerol from ldlp during incubation. the "lipase-treated ldlp" thus obtained still contained associated apolipophorin-iii (apolp-iii). these data suggest that the dissociation of apolp-i ... | 1992 | 1478928 |
| analysis of the flagellin (hag) gene of alkalophilic bacillus sp. c-125. | motility of the alkalophilic bacillus sp. c-125, a flagellate bacterium, was demonstrated to be na(+)- and ph-dependent. flagellin protein from this strain was purified to homogeneity and the n-terminal sequence determined. using the hag gene of bacillus subtilis as a probe, the hag gene of bacillus sp. c-125 was identified and cloned into escherichia coli. sequencing of this hag gene revealed that it encodes a protein of 272 amino acids (m(r) 29,995). the predicted n terminal sequence of this p ... | 1992 | 1479346 |
| characterization of an anaerobic fungus from llama faeces. | an anaerobic fungus was isolated from llama faeces. based on its morphological characteristics, polyflagellated zoospores, extensive rhizoid system and the formation of monocentric colonies, the fungus is assigned to the genus neocallimastix. neocallimastix sp. l2 is able to grow on several poly-, oligo- and monosaccharides. it differs from other neocallimastix isolates in its inability to ferment inulin. neocallimastix sp. l2 requires co2 for growth. in the presence of 100% co2 in the gas phase ... | 1992 | 1479350 |
| campylobacter helveticus sp. nov., a new thermophilic species from domestic animals: characterization, and cloning of a species-specific dna probe. | an atypical group of thermophilic catalase-negative campylobacter strains, the 'ch' (swiss) group, can be recovered from faeces of domestic cats and dogs after selection by filtration, or with the antibiotic cefoperazone. this group of strains shows no relative dna homology with any species in rrna superfamily vi (vandamme et al., 1991, international journal of systematic bacteriology 41, 88-103) except with four thermophilic campylobacter species, notably c. upsaliensis. the group is homogeneou ... | 1992 | 1479353 |
| antifungal activity of meridine, a natural product from the marine sponge corticium sp. | meridine [1], a polycyclic alkaloid derived from the marine sponge corticium sp., was found to inhibit the growth of candida albicans and cryptococcus neoformans. activity was also observed against trichophyton mentagrophytes and epidermophyton floccosum. studies of the mechanism of action of this agent have shown an inhibition of nucleic acid biosynthesis. | 1992 | 1479383 |
| differential molecular mechanism of the estrogen action that regulates lactoferrin gene in human and mouse. | the 5'-flanking region of the human lactoferrin gene was isolated from a human placental genomic library. this genomic clone contains a 16-kilobase pair (kbp) insert and produces seven fragments when digested with the saci restriction enzyme. we sequenced one of the fragments that comprises 1294 bp of the 5'-flanking sequence, 79 bp of the first exon, and 690 bp of the first intron. a major transcription start site was mapped by primer extension. the region immediately upstream from the transcri ... | 1992 | 1480183 |
| mycotoxic flora and mycotoxins in smoke-dried fish from sierra leone. | examination of 20 samples of smoke-dried fish of the ethmolosa sp. commonly called "bonga", from homes and markets in njala (sierra leone) revealed the presence of 4 aspergilli species: a. flavus links ex fries, a. ochraceus wilhelm, a. tamarii kita and a. niger van tieghem. fresh or properly preserved smoke-dried fish showed no signs of fungal contamination. mouldy fish extracts contained varying amounts of aflatoxins b1, g1, g2 and ochratoxin a. isolates of a. flavus grown on yeast extract suc ... | 1992 | 1480216 |
| [isolation and characterization of mutactimycin-producing mutant]. | natural non-antibiotic producing streptomyces sp. 1254 was mutagenized by uv irradiation and two active mutants were isolated. mutant 113 produced novel anthracycline compounds designated mutactimycins. mutactimycin a was active against the bacteriophage of bac. subtilis and some viruses in tissue culture. the mutant 2-6 synthesized a basic water-soluble antimicrobial antibiotic. chemical analysis of the whole cell hydrolysate and the morphological characterization showed that the strain 1254 an ... | 1992 | 1481530 |
| [the use of a method for the electrophoretic typing by protein profiles of bacteria in the genus acinetobacter for epidemiological diagnosis]. | on the basis of data obtained by the biological characterization and electrophoretic typing of acinetobacter sp. cultures by the spectra of membrane proteins epidemiological relationships, routes of transmission and specific features of the circulation of strains causing purulent inflammatory diseases have been determined. | 1992 | 1481602 |
| prostaglandins do not mediate impaired autoregulation or increased renin secretion in remnant rat kidneys. | the rat remnant kidney model, produced by approximately five-sixths reduction in functional renal mass, is characterized by renal vasodilation, impaired autoregulation, and increased activity of the renin-angiotensin system. the present studies were designed to investigate the role of vasodilatory prostaglandins (pgs) in the altered hemodynamics in the remnant kidney. four weeks post-ablation, renal blood flow (rbf), was significantly higher in rats fed a standard protein (sp) diet (n = 16) comp ... | 1992 | 1481882 |
| av3v lesion impairs responses induced by cholinergic activation of sfo in rats. | this study was performed to investigate the effect of lesion of the anteroventral third ventricle (av3v) region on the pressor, bradycardic, dipsogenic, natriuretic, kaliuretic, and antidiuretic responses induced by cholinergic activation of the subfornical organ (sfo) in rats. male holtzman rats with sham or electrolytic av3v lesion were implanted with a stainless steel cannula directly into the sfo. microinjection of the cholinergic agonist carbachol (2 nmol) into the sfo of sham rats induced ... | 1992 | 1481939 |
| identification of sulphate-reducing ectosymbiotic bacteria from anaerobic ciliates using 16s rrna binding oligonucleotide probes. | the identity of ectosymbiotic bacteria of some marine, free-living anaerobic ciliates (metopus contortus, caenomorpha levanderi and parablepharisma sp.) was studied using fluorescent-dye-conjugated oligonucleotides complementary to short sequence elements of 16s ribosomal rna. the ectosymbiotic bacteria of all species hybridized with a eubacterial probe and those of the two former mentioned species hybridized with a general probe for sulphate-reducing bacteria, but not to a probe specific for de ... | 1992 | 1482269 |
| maleylacetate reductase of pseudomonas sp. strain b13: dechlorination of chloromaleylacetates, metabolites in the degradation of chloroaromatic compounds. | the maleylacetate reductase of 3-chlorobenzoate-grown cells of pseudomonas sp. strain b13 has been purified 50-fold. the enzyme converted 2-chloromaleylacetate to 3-oxoadipate with temporary occurrence of maleylacetate; 1 mol of chloride was eliminated during the conversion of 1 mol of 2-chloro- and 2,3-dichloromaleylacetate; 2 mol of nadh were consumed per mol of 2-chloro- and 2,3-dichloromaleylacetate while only 1 mol was necessary to catalyze the conversion of maleylacetate or 2-methylmaleyla ... | 1992 | 1482270 |
| characterisation of genes encoding two novel members of the aldo-keto reductase superfamily. | the predicted amino acid sequence of the protein encoded by a cdna clone isolated from the protozoan haemoparasite babesia bovis has approximately 22% amino acid identity with the pichia stipitis xylose reductase. there are similar levels of amino acid identity with other members of the aldo-keto reductase superfamily. the identities include many residues highly conserved in the superfamily. however, the amino acid sequence of the b. bovis protein (akr1) clearly lies outside the cluster of the p ... | 1992 | 1482401 |
| synthesis and biological activity of new heterocyclic structures: [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c] [1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo [4', 5': 4,5] pyrimido[6,1-b][1,3]thiazine. | some derivatives of thiazolo[3,2-c]pyrimidine, pyrimido[6,1-b][1,3]thiazine, thiazolo[2,3-i]purine, [1,3]thiazino[2,3-i]purine, thiazolo[3,2-c][1,2,3]triazolo[4,5-e]pyrimidine and [1,2,3]triazolo[4',5':4,5]pyrimido[6,1-b][1,3]thiazine were prepared. the compounds were tested for antimicrobial and antimycotic activity on a number of strains, namely, escherichia coli, proteus vulgaris, p. mirabilis, pseudomonas aeruginosa, salmonella sp., staphylococcus aureus, streptococcus faecalis, bacillus sub ... | 1992 | 1482518 |
| effects of nimodipine on brain blood flow following acute brain ischemia in the newborn piglet. | we examined the effect of the calcium channel blocker nimodipine on postischemic hypoperfusion in the newborn piglet brain. a severe pneumothorax (sp) was induced by injecting air into the right thorax until the mean arterial blood pressure fell to 25% of baseline and was maintained for 4 min. blood flow was immediately reduced 70-90% from baseline in each brain region during sp. in untreated animals postischemic hypoperfusion existed at 60 min, following recovery from sp with regional brain blo ... | 1992 | 1483364 |
| differential expression of nods accounts for the varied abilities of rhizobium fredii usda257 and rhizobium sp. strain ngr234 to nodulate leucaena spp. | transfer of a cosmid containing nodsu from rhizobium sp. ngr234 to rhizobium fredii usda257 expands the host range for nodulation to include the perennial tropical legumes, leucaena leucocephala and leucaena diversifolia. complementation experiments with a series of subclones established that nods and its associated nod-box promoter from ngr234 are sufficient to confer this extended host-range phenotype to l. leucocephala. strain usda257 contains its own copy of nodsu, including upstream nod-box ... | 1992 | 1484488 |
| two new l-serine variants of microcystins-lr and -rr from anabaena sp. strains 202 a1 and 202 a2. | two new microcystins, [l-ser7]microcystin-lr (1) and [l-ser7]microcystin-rr (2), were isolated from a filamentous fresh water cyanobacterium (blue-green alga), anabaena sp. strain 202 a1, along with the two major toxins, [dha7]microcystin-lr (3) and [dha7]microcystin-rr (4) and their minor components the d-asp variants [d-asp3,dha7]microcystin-lr (5) and [d-asp3,dha7]microcystin-rr (6). anabaena sp. strain 202 a1 also produced another new toxin, whose structure is tentatively proposed as [d-asp3 ... | 1992 | 1485340 |
| infectivity of moniezia benedeni and moniezia expansa to oribatid mites from ohio and georgia. | six species of oribatid mites belonging to four families and five genera were successfully infected by m. benedeni. they were scheloribates laevigatus (scheloribatidae), exoribatula sp. cf. biundatus (scheloribatidae), xylobates capucinus (haplozetidae) and zygoribatula undulata (oribatulidae) from ohio, and galumna ithacensis (galumnidae) and scheloribates lanceoliger (scheloribatidae) from georgia. cysticercoids were also recovered from s. laevigatus infected with m. expansa. among the six spe ... | 1992 | 1485410 |
| epidemiology of trichostrongylidosis in cattle: development of infective larvae in the second part of the grazing period and their contribution to infections in the same year. | a grazing experiment was performed in the swiss midland region with 22 first-season calves which were grazed together from may to mid july when they were allocated to two equal groups, one receiving a morantel sustained-release trilaminate bolus (paratect flex) bolus, pfizer, zurich, switzerland) and the other one remaining as an untreated control. each group was moved to a clean pasture on 21 july. the larval contamination on the pasture with the control animals reached 4652 third stage larvae ... | 1992 | 1485412 |
| structure and functions of a dimeric form of surfactant protein sp-c: a fourier transform infrared and surfactometry study. | surfactant proteins sp-b (m(r) = 8700, reduced) and sp-c (m(r) = 3000-6000, major form, non-reduced) interact with surfactant phospholipids to enhance their surface active properties. in the present study, we describe the structural and functional characteristics of a novel dimeric form of bovine sp-c (m(r) = 9000, non-reduced), which is identified as [sp-c]2. dimeric sp-c exhibits surface tension-lowering properties differing from those of monomeric sp-c and enhances the surface properties of b ... | 1992 | 1486663 |
| monitoring of serum markers for fibrosis during ccl4-induced liver damage. effects of anti-fibrotic agents. | liver fibrosis was induced in rats by repeated peritoneal injections of carbon tetrachloride (ccl4) over a period of 2-11 weeks. serum procollagen iii peptide (spiiinp), prolidase (sp) and alanine aminotransferase (salt) levels were monitored during the period of induction. the extent of fibrosis was semi-quantitatively estimated after collagen staining, and the anti-fibrotic effects of 16,16-dimethyl prostaglandin e2 (dmpge2), colchicine, and zinc sulphate were studied. spiiinp and sp were incr ... | 1992 | 1487604 |
| vancomycin kinetics in plasma and synovial fluid following intravenous administration in horses. | vancomycin hydrochloride was infused intravenously (i.v.) over a 30-min period in five horses at doses of 6.6, 11.0 and 15.4 mg/kg. vancomycin concentration in plasma and synovial fluid samples was measured using a polarization immunoassay. a pharmacokinetic model was developed to accommodate the special features of the present study. the data were described by a two compartment open model with synovial fluid as an additional compartment in exchange with plasma. minimum inhibitory concentration ... | 1992 | 1487834 |
| cell membranes and multilamellar vesicles: influence of ph on solvent induced damage. | pigment leakage from sheep and horse erythrocytes and from red beet tissue induced by non-polar solvents was determined as a function of ph. the results were compared to disruption of multilamellar vesicles (mlv) composed of phospholipids with equimolar cholesterol under identical conditions of solvent exposure and ph. solvent access to cholesterol was used to measure vesicle disruption. mlv were made from 1,2-dioleoyl phosphatidylethanolamine, sphingomyelin (sp) and various phosphatidylcholines ... | 1992 | 1487967 |
| [imipenem in the treatment of patients with severe surgical infection]. | the clinical efficacy and safety of intravenously administered imipenem/cilastatin in the treatment of 45 patients with severe bacterial septicemia due to intra-abdominal abscesses, respiratory and urinary tract as well as skin, soft tissue and bone infections was studied in the prospective and open trial. the in vitro antimicrobial activity of imipenem has been assessed on the basis of 909 bacterial strains isolated from patients treated and non-treated with imipenem/cilastatin. among them were ... | 1992 | 1488347 |
| treatment and developmental therapeutics in aspergillosis. 1. amphotericin b and its derivatives. | in recent years, the frequency of infections caused by aspergillus sp. has been on the rise. immunocompromised patients are especially vulnerable to such infections. the polyene antibiotic amphotericin b is currently considered to be therapeutically the most effective drug against aspergillus-induced infections. in the present review, the clinical efficacy of amphotericin b, its toxicities and various routes of applications, are discussed. different combinations of amphotericin b with other drug ... | 1992 | 1488564 |
| cholinergic microstimulation of the peribrachial nucleus in the cat. i. immediate and prolonged increases in ponto-geniculo-occipital waves. | the cholinergic agonist carbachol was injected into the pontine pb area where pgo bursting cells have been recorded. when microinjections were localized to the ventrolateral aspect of the caudal pb nucleus near aggregates of chat immunolabeled cholinergic neurons, carbachol produced an immediate onset of state-independent pgo waves in the ipsilateral lgb. these state-independent pgo waves persisted for 3-4 days. after the first 24 hrs pgo wave activity increasingly became associated with rem sle ... | 1992 | 1489248 |
| cholinergic microstimulation of the peribrachial nucleus in the cat. ii. delayed and prolonged increases in rem sleep. | the hypothesis that rem sleep is cholinergically mediated is supported by the identification of a cholinoceptive trigger zone in the ftg. since this trigger zone is devoid of cholinergic neurons, the aim of the present study was to test the hypothesis that a cholinergic drive for rem sleep may come from the cholinergic cells of the pbl region. chronically implanted freely moving cats with electrodes for sleep and pgo wave recordings were used. guide tubes were implanted for carbachol microinject ... | 1992 | 1489249 |
| dactylocyclines, novel tetracycline derivatives produced by a dactylosporangium sp. i. taxonomy, production, isolation and biological activity. | a screen for antibiotics with activity against tetracycline-resistant microorganisms has led to the isolation of dactylosporangium sp. (atcc 53693), a producer of several novel tetracycline derivatives. the major fermentation products, dactylocyclines a and b, were purified and mic values determined against tetracycline-resistant and tetracycline-sensitive gram-positive bacteria. the dactylocyclines represent the first naturally occurring tetracycline c2 amides which lack cross resistance with t ... | 1992 | 1490880 |
| dactylocyclines, novel tetracycline derivatives produced by a dactylosporangium sp. ii. structure elucidation. | fermentation of dactylosporangium sp. (atcc 53693) produces a mixture of tetracycline derivatives from which several related tetracycline glycosides, the dactylocyclines, were isolated and their structures determined. the most abundant glycoside in initial fermentations was found to be dactylocycline a. each glycoside proved to be acid sensitive and readily hydrolyzed to a common aglycone, dactylocyclinone. while the aglycone was cross resistant with tetracycline, the dactylocyclines proved acti ... | 1992 | 1490881 |
| dactylocyclines, novel tetracycline derivatives produced by a dactylosporangium sp. iii. absolute stereochemistry of the dactylocyclines. | the dactylocycline antibiotics were found through circular dichroism measurements, nmr spectroscopy and chemical transformations to possess the usual tetracycline family absolute configuration at carbons 4, 4a, 5a and 12a. the absolute stereochemistry about the c-6 carbon, however, was the reverse of that found with previously investigated tetracyclines. | 1992 | 1490882 |
| identification of the disulfide bonds in human plasma protein sp-40,40 (apolipoprotein-j). | sp-40,40, a human plasma protein, is a modulator of the membrane attack complex formation of the complement system as well as a subcomponent of high-density lipoproteins. in the present study, the positions of the disulfide bonds in sp-40,40 were determined. sp-40,40 was purified from human seminal plasma by affinity chromatography using an anti-sp-40,40 monoclonal antibody and reversed-phase, high-performance liquid chromatography (hplc). the protein was digested with trypsin and the fragments ... | 1992 | 1491011 |
| parasites of cottontail rabbits of southern illinois. | fifteen species of parasites including haemaphysalis leporispalustris, ixodes dentatus, amblyomma americanum, cediopsylla simplex, odontopsyllus multispinosus, cuterebra sp., obeliscoides cuniculi, trichostrongylus calcaratus, trichostrongylus affinis, longistriata noviberiae, dermatoxys veligera, trichuris sp., mosgovoyia sp., taenia pisiformis, and hasstilesia tricolor as well as coccidia oocysts were collected from 96 cottontail rabbits (sylvilagus floridanus) confined to a pen in southern il ... | 1992 | 1491303 |
| fungal contamination of kashar cheese in turkey. | fifty random samples of kashar cheese were collected from shops in different localities in erzurum, all contained moulds. mean count of total surface mould was 3.02 x 10(10)/g cheese and that of inner mould was 3.02 x 10(3)/g cheese. the genera penicillium, aspergillus, mucor, rhizopus and geotrichum sp. were isolated from cheese samples. aflatoxins were not detected in cheese samples. potassium sorbate inhibited mould growth and sporulation in yes broth. the public health importance and economi ... | 1992 | 1491710 |
| [nosocomial infection in newborn infants on mechanical ventilation]. | during a two-year period (1988-1989), were studied newborns treated with intermittent mandatory ventilation (imv). the goal was to know incidence of infections in these patients and characteristics of these process. of 187, 40 (21.3%) were infected. for these neonates the mean gestational age was 33 +/- 3 weeks and average for birth weight was 1755 +/- 644 g. in 32 the infecting agent was isolated, being the most common coagulase negative staphylococcus, it was found in 18 (45%), klebsiella sp i ... | 1992 | 1492914 |
| bacteriology, drug stability and exchange of percutaneous delivery systems and antibacterial filters in long-term intrathecal infusion of opioid drugs and bupivacaine in "refractory" pain. | to provide a basis for recommendations on the exchange of containers (syringes and cassettes) and antibacterial filters, and for choice of administration device in patients with "refractory" pain treated with long-term percutaneous intrathecal (it) infusions of opioid (morphine or buprenorphine) and bupivacaine mixtures. | 1992 | 1493343 |
| organization and nature of fortimicin a (astromicin) biosynthetic genes studied using a cosmid library of micromonospora olivasterospora dna. | the cloning of five dna segments carrying at least seven genes (fms1, fms3, fms4, fms5, fms7, fms11, and fms12) that participate in fortimicin a (astromicin) biosynthesis was described previously. these dna fragments were used to screen a cosmid library of genomic dna in order to examine if these biosynthetic genes are clustered in micromonospora olivasterospora. one cosmid clone (pglm990) was obtained, which hybridized to all the probes. complementation analysis, using mutants blocked at variou ... | 1992 | 1494349 |
| n-formimidoyl fortimicin a synthase, a unique oxidase involved in fortimicin a biosynthesis: purification, characterization and gene cloning. | micromonospora olivasterospora, a fortimicin a (ftm a, astromicin) producer, was found to carry an enzyme that converts ftm a to n-formimidoyl ftm a (fi-ftm a). this enzyme (fi-ftmase) was purified to homogeneity and shown to be a flavin adenine dinucleotide (fad) enzyme. tracer experiments proved that the formimidoyl group was derived from c-2 of glycine via oxidation of the amino acid in the presence of ftm a and oxygen. the gene encoding this enzyme, fms 14, was cloned using a 26-mer oligonuc ... | 1992 | 1494350 |
| habenula and thalamus cell transplants mediate different specific patterns of innervation in the interpeduncular nucleus. | innervation of specific peptidergic and cholinergic compartments of the interpeduncular nucleus (ipn) was investigated using embryonic cell suspension transplants immunoreactive for substance p (sp) and chat. in both neonatal and adult host rats, the ipn was first denervated of its normal sp and cholinergic input from the medial habenula by bilateral lesions of the fasciculi retroflexi (fr). in adult hosts, transplants of embryonic habenular cells placed near the denervated ipn mediated a return ... | 1992 | 1494956 |
| habenula and thalamus cell transplants restore normal sleep behaviors disrupted by denervation of the interpeduncular nucleus. | the preceding companion study (eckenrode et al., 1992) showed that cell suspension transplants of fetal habenula cells placed near the interpeduncular nucleus (ipn) following lesions of the fasciculus retroflexus (fr) restore the normal pattern of substance p (sp) staining in habenular target subnuclei of the ipn in both perinatal and adult hosts, and restore chat staining in the ipn of perinatal hosts. similarly placed transplants of fetal thalamus cells only restore chat staining in the ipn of ... | 1992 | 1494957 |
| regulatory sequences for expressing genes in oomycete fungi. | promoter and terminator sequences from a range of species were tested for activity in the oomycetes, a group of lower fungi that bear an uncertain taxonomic affinity to other organisms and in which little is known of the sequences required for transcription. transient assays, using the reporter gene beta-glucuronidase (gus), were used to examine the function of these promoters and terminators in the plant pathogens phytophthora infestans and p. megasperma f. sp. glycinea, and in the saprophytic ... | 1992 | 1495476 |
| ultrastructure of the developing protonephridial system of the cercaria of philophthalmus sp. (trematoda, digenea). | the fully developed flame bulb of philopthalmus exhibits the structure characteristic of trematoda and monogenea: external and internal ribs forming a weir, external and internal leptotriches, and two longitudinal cytoplasmic cords connected by a septate junction. the proximal canal has a septate junction and surface lamellae. in developing cercariae, perikarya of terminal and proximal canal cells are close together, and sheet-like outgrowths of the terminal cell are externally surrounded by cyt ... | 1992 | 1495914 |
| phycocyanin alpha-subunit phycocyanobilin lyase. | phycobiliproteins, unlike other light-harvesting proteins involved in photosynthesis, bear covalently attached chromophores. the bilin chromophores are attached through thioether bonds to cysteine residues. the cyanobacterium synechococcus sp. pcc 7002 has eight distinct bilin attachment sites on seven polypeptides, all of which carry the same chromophore, phycocyanobilin. when two genes in the phycocyanin operon of this organism, cpce and cpcf, are inactivated by insertion, together or separate ... | 1992 | 1495995 |
| brain neuropeptides: changes by treatment with the convulsants pentylenetetrazole and bicuculline. | 1. the effects of chemically induced convulsions, clinically similar to those elicited by electroconvulsive treatment (ect), on brain regional distribution of neuropeptide y-, neurokinin a-, substance p- and neurotensin-like immunoreactivities were studied in the rat. 2. pentylenetetrazole (ptz) and bicuculline (bic) were used to induce grand mal seizures. rats were divided into three groups receiving one of the following treatments: saline, ptz (45 mg/kg) or bic (1.5 mg/kg). 3. after sacrifice ... | 1992 | 1496130 |
| uranium bioaccumulation by a citrobacter sp. as a result of enzymically mediated growth of polycrystalline huo2po4. | a citrobacter sp. accumulates heavy deposits of metal phosphate, derived from an enzymically liberated phosphate ligand. the cells are not subject to saturation constraints and can accumulate several times their own weight of precipitated metal. this high capacity is attributable to biomineralization; uranyl phosphate accumulates as polycrystalline huo2po4 at the cell surface. the precipitated metal is indistinguishable from crystalline huo2po4.4h2o grown by chemical methods. | 1992 | 1496397 |
| immunisation of cattle against theileriosis in nakuru district of kenya by infection and treatment and the introduction of unconventional tick control. | one hundred and one cross european-boran cattle (50 cows and 51 calves), on a farm in nakuru district, kenya, were immunised against theileriosis using theileria parva lawrencei and theileria parva parva stocks from another district of kenya. the stabilates used were t.p.lawrencei (mara iii) used at 10(-1.7) dilution and t.p.parva (kilae) used at 10(-1.0) dilution. the stabilates were combined and inoculated simultaneously with a short-acting formulation of oxytetracycline hydrochloride given in ... | 1992 | 1496782 |
| serum and skin concentrations after multiple-dose oral administration of trimethoprim-sulfadiazine in dogs. | six healthy adult mixed breed dogs were each given 5 oral doses of trimethoprim (tmp)/sulfadiazine (sdz) at 2 dosage regimens: 5 mg of tmp/kg of body weight and 25 mg of sdz/kg every 24 hours (experiment 1) and every 12 hours (experiment 2). serum and skin concentrations of each drug were measured serially throughout each experiment and mean serum concentrations of tmp and sdz were determined for each drug for 24 hours (experiment 1) and 12 hours (experiment 2) after the last dose was given. in ... | 1992 | 1497202 |
| interfacial adsorption of simple lipid mixtures combined with hydrophobic surfactant protein from pig lung. | hydrophobic pulmonary surfactant protein enriched in sp-c has been mixed in amounts up to 10% by weight with various phospholipids. the lipids used were dipalmitoyl phosphatidylcholine (dppc), or dppc plus unsaturated phosphatidylglycerol (pg), or phosphatidylinositol (pi) in molar ratios of 9:1 and 7:3. the protein enhanced the rate and extent of adsorption of each lipid preparation into the air-water interface, and its respreading after compression on a surface balance. maximum surface pressur ... | 1992 | 1497860 |
| [bacteriology of mucoviscidosis during a 5-year period]. | we have studied the bacteriology of mucoviscidosis cases from 1985 to 1989. | 1992 | 1498172 |
| [surgical bacteremia. analysis of 210 episodes with special attention to factors influencing prognosis]. | from january 1984 to april 1987, we have prospectively studied 210 consecutive episodes of bacteremia recorded in patients who underwent major surgical procedures. the incidence rate was 6.4 episodes/1000 surgical procedures. men were responsible of 73.8% of episodes. the highest incidence was recorded in general surgery patients and the lowest in ob & gyn patients. bacteremia-related mortality was 15.2% (overall mortality 29.5%). the five most common microorganisms isolated were: staphylococcus ... | 1992 | 1498174 |
| [evaluation of a rapid technique for detecting the type a streptococcus antigen (test pack strep a)]. | to test a rapid technique of antigen detection (tad) of type a betahemolitic streptococci (bhsa) in acute tonsillitis (at), comparing it with and using as a point of reference traditional blood agar cultivation (bac). additionally, the use of clinical data as a method of supposed diagnosis was studied. | 1992 | 1498218 |
| mycetoma of the foot due to fusarium sp. treated with oral ketoconazole. | we report the case of a 30-year-old nigerian patient with mycetoma of the foot without bone involvement caused by fusarium sp..long-term administration of ketoconazole produced a significant improvement with good clinical and biological tolerance. | 1992 | 1498403 |
| purification and characterization of a thermostable proteinase isolated from thermus sp. strain rt41a. | thermus sp. strain rt41a produces an extracellular thermostable alkaline proteinase. the enzyme has a high isoelectric point (10.25-10.5) which can be exploited in purification by using cation-exchange chromatography. the proteinase was purified to homogeneity and has a molecular mass of 32.5 kda by sds/page. it is a glycoprotein, containing 0.7% carbohydrate as glucose equivalents, and has four half-cystine residues present as two disulphide bonds. maximum proteolytic activity was observed at p ... | 1992 | 1499549 |
| transferable antibiotic resistance among thermotolerant coliforms from rural drinking water in india. | a total of 231 thermotolerant coliforms was isolated from rural drinking water from four states of india. of these, 220 isolates were resistant to ampicillin, chloramphemicol, streptomycin and tetracycline. multiple (mar), double and single antibiotic resistances were observed in 31.4, 48.6 and 13.7% of the isolates, respectively. out of 177 antibiotic-resistant isolates examined for transmissibility, only 15.3% were able to transfer their resistances to escherichia coli k-12 recipient. the resi ... | 1992 | 1499665 |
| markedly reduced beta-cell function does not result in insulin resistance in islet autografted dogs. | autotransplantation of islets of langerhans has resulted in long-term normoglycemia in pancreatectomized dogs. this canine model is useful in evaluating both the progress of islet transplantation and the effect of a reduced islet mass upon the determinants of glucose tolerance: i.e., insulin secretion, insulin sensitivity, and glucose effectiveness. to determine the effect of a reduced islet mass on these factors, we measured the acute insulin response to arginine (aira) and glucose (airg), the ... | 1992 | 1499868 |
| selection of the chimpanzee over the baboon as a model for helicobacter pylori infection. | baboons (papio sp.) and chimpanzees (pan troglodytes) were screened for the presence of helicobacter pylori. the gastric mucosae of the baboons were colonized by large spiral bacteria. however, a group of adult chimpanzees were identified that were free of spiral gastric bacteria, with five animals being recruited into an h. pylori challenge study. these animals were inoculated orogastrically with one of four strains of h. pylori and followed for up to 26 weeks. h. pylori was established in one ... | 1992 | 1499934 |
| identification and purification of a second form of cu/zn superoxide dismutase from schistosoma mansoni. | our laboratories previously isolated a putative extracellular or membrane-associated cu/zn superoxide dismutase (cu/zn-sod) gene, designated a signal peptide-containing (sp) cu/zn-sod, from schistosoma mansoni. sod activity was thus investigated throughout the life cycle of s. mansoni and found in all stages: eggs, miracidia, cercariae, schistosomula, lung-stage worms, and adult worms. the adult worms had the highest sod activity (53 +/- 9 nitrite units), which was five times higher than that of ... | 1992 | 1500173 |
| isolation and characterization of new peptide antibiotics, plusbacins a1-a4 and b1-b4. | new antibiotics, plusbacins a1-a4 and b1-b4, were isolated from the culture broth of a strain of pseudomonas sp. these antibiotics were isolated as a complex by column chromatographies on diaion hp-20 and silica gel, and then separated by hplc. they are amphoteric in nature. the hydrochlorides are obtained as colorless powders, soluble in methanol and alkaline water. from their physico-chemical properties, these antibiotics are presumed to be acyloctapeptides containing a lactone linkage, and th ... | 1992 | 1500345 |
| pi-200 and pi-201, new platelet aggregation inhibitors produced by streptomyces sp. a7498. taxonomy, fermentation, isolation, physico-chemical properties, structure determination and biological properties. | two new platelet aggregation inhibitors, pi-200 and pi-201 were isolated from the fermentation broth of streptomyces sp. a7498. pi-200 and pi-201 inhibited adp-induced aggregation of rabbit platelets with an ic50 of 3.8 x 10(-4) m and 7.1 x 10(-4) m, respectively. | 1992 | 1500356 |
| excretory-secretory antigenic components of paragonimus heterotremus recognized by infected human sera. | antigenic components of paragonimus heterotremus metabolic products were revealed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) and immunoblot analysis of sera from patients with p. heterotremus infection, from patients with other illnesses, and from healthy adults. by sds-page, it was found that the metabolic products comprised more than eight major polypeptides. immunoblot analysis revealed 11 components which were strongly recognized by paragonimiasis antisera. these ... | 1992 | 1500515 |
| a comparative analysis of streaming potentials in vivo and in vitro. | streaming potentials (sps) measured in vivo at a specific site on intact cortical bone (canine tibia) have been compared with measurements from the same site in vitro, tested as an excised bone strip soaked in hank's balanced salt solution. the amplitude of sps per periosteal strain in vitro was larger in 13 tibias than in vivo (by an average x6.5 at 1 hz), but values per transcortical strain difference were similar. in vitro, sp magnitudes rose more sharply to an asymptotic value as a function ... | 1992 | 1500983 |
| protein quality of developed home made weaning foods. | home made weaning foods developed from locally available foods like bajra, barley, green gram (vigna radiata l.), amaranth grain (amaranthus sp.) and jaggery using household technologies like roasting and malting had a per ranging from 2.04 to 2.13, bv 79.56 to 80.68, npu 66.75 to 67.86, npr 2.13 to 2.76 and pre 34.18 to 44.18. the values were comparable to that of cerelac--a commercial weaning food. | 1992 | 1502125 |
| effect of spermine on sperm capacitation of guinea pig in vitro. | spermine (sp) 10(-5) mm had vigorous activity of guinea pig spermatozoa, while it completely abolished sperm forward motility (sfm) at a concentration of 10(-3) mm. there appeared to be a dose relationship to inhibition to motility. 2-difluoromethylornithine 10 mm antagonized the sp-induced inhibition of sfm after 3 h of incubation. capacitation of a guinea pig sperm was inhibited by sp in a concentration-dependent manner. the majority of acrosome-reacted sperm did not display hyperactivated mot ... | 1992 | 1503522 |
| polyphasic taxonomic study of the emended genus arcobacter with arcobacter butzleri comb. nov. and arcobacter skirrowii sp. nov., an aerotolerant bacterium isolated from veterinary specimens. | the relationships of 77 aerotolerant arcobacter strains that were originally identified as campylobacter cryaerophila (now arcobacter cryaerophilus [p. vandamme, e. falsen, r. rossau, b. hoste, p. segers, r. tytgat, and j. de ley, int. j. syst. bacteriol. 41:88-103, 1991]) and 6 reference strains belonging to the taxa arcobacter nitrofigilis, arcobacter cryaerophilus, and "campylobacter butzleri" were studied by using a polyphasic approach, in which we performed dna-rrna hybridizations, dna-dna ... | 1992 | 1503968 |
| mycoplasma penetrans sp. nov., from the urogenital tract of patients with aids. | an unusual mycoplasma, which was isolated from the urine of a human immunodeficiency virus-positive male homosexual patient, has an elongated flask shape and two unique sharply divided internal compartments. the tiplike compartment is densely packed with fine granules, and the body compartment is loosely filled with coarse granules consistent with ribosomal structures. the organism has properties of adherence, hemadsorption, and cytadsorption and invades many different types of mammalian cells. ... | 1992 | 1503969 |
| enterococcus flavescens sp. nov., a new species of enterococci of clinical origin. | four yellow-pigmented group d enterococci of uncertain taxonomic position were isolated from several humans with severe infections. the results of dna composition, dna-dna hybridization, fatty acid content, and biochemical property studies demonstrated that these organisms were slightly related to other previously described yellow-pigmented enterococcal species and constitute a new species, for which we propose the name enterococcus flavescens. the type strain of e. flavescens is strain ccm 4239 ... | 1992 | 1503970 |
| legionella shakespearei sp. nov., isolated from cooling tower water. | a legionella-like organism (strain 214t [t = type strain]) was isolated from a cooling tower in stratford-upon-avon, england. this strain required l-cysteine and contained cellular branched-chain fatty acids that are typical of the genus legionella. strain 214t produced pink colonies on buffered charcoal-yeast extract agar. ubiquinone q-12 was the major quinone. strain 214t was serologically distinct from other legionellae as determined by a slide agglutination test. the results of dna hybridiza ... | 1992 | 1503972 |
| description of porphyromonas circumdentaria sp. nov. and reassignment of bacteroides salivosus (love, johnson, jones, and calverley 1987) as porphyromonas (shah and collins 1988) salivosa comb. nov. | a new species, porphyromonas circumdentaria, is proposed for pigmented, asaccharolytic strains that were isolated from the gingival margins or mouth-associated diseases of cats. this bacterium is an obligately anaerobic, gram-negative, brown- or black-pigmented, asaccharolytic, nonmotile, nonsporing, rod-shaped organism which does not grow in bile and has a guanine-plus-cytosine content of 40 to 42 mol%. it produces major amounts of acetic, butyric, and isovaleric acids and minor amounts of prop ... | 1992 | 1503973 |
| flexibacter ovolyticus sp. nov., a pathogen of eggs and larvae of atlantic halibut, hippoglossus hippoglossus l. | a psychrotrophic flexibacter sp., flexibacter ovolyticus sp. nov., was isolated from the adherent bacterial epiflora of atlantic halibut (hippoglossus hippoglossus l.) eggs and was shown to be an opportunistic pathogen for halibut eggs and larvae. the strains which we isolated had the enzymatic capacity to dissolve both the chorion and the zona radiata of the egg shells. a total of 35 isolates were characterized by using morphological and biochemical tests. these strains were rod shaped, gram ne ... | 1992 | 1503974 |
| taxonomic study of the lactobacillus acidophilus group, with recognition of lactobacillus gallinarum sp. nov. and lactobacillus johnsonii sp. nov. and synonymy of lactobacillus acidophilus group a3 (johnson et al. 1980) with the type strain of lactobacillus amylovorus (nakamura 1981). | biochemical properties and dna-dna reassociation studies of lactobacillus acidophilus strains isolated from humans and animals indicate that these include six genomospecies. two new species can be differentiated from the established species of the genus lactobacillus: l. gallinarum sp. nov. (type strain, atcc 33199) and l. johnsonii sp. nov. (type strain, atcc 33200). furthermore, it was clarified that l. acidophilus group a3 (johnson et al. 1980) is synonymous with l. amylovorus. | 1992 | 1503977 |
| carbohydrate cytochemistry of bonnet monkey (macaca radiaca) nasal epithelium. response to ambient levels of ozone. | the main purpose of this study was to determine whether exposures to ambient levels of ozone induced changes in the carbohydrate content in the anterior nasal cavity of bonnet monkey. monkeys were exposed to 0.00. 0.15, or 0.30 ppm of ozone for 6 or 90 days, 8 hours/day. monkeys treated for 90 days to 0.15 or 0.30 ppm of ozone displayed damaged ciliated cells and a new cell type in their respiratory nasal epithelium, the "intermediate" cells possessing morphological characteristics of both cilia ... | 1992 | 1504469 |
| effects of brl 38227 on neurally-mediated responses in the guinea-pig isolated bronchus. | 1. in guinea-pig isolated bronchus treated with indomethacin (2.8 microm), electrical field stimulation (efs; 10 hz, 0.5 ms, 60-70 v, for 10 s) evoked a tetrodotoxin (3 microm)-sensitive, biphasic contraction comprising a rapid, atropine (1 microm)-sensitive cholinergic response succeeded by a slowly developing, capsaicin (10 microm)-sensitive, non-adrenergic, non-cholinergic excitatory (nance) response. 2. brl 38227 (0.3-3 microm), salmeterol (0.003-3 microm) and ketotifen (1.0-300 microm) each ... | 1992 | 1504720 |
| the effects of a- and c-fiber stimulation on patterns of neuropeptide immunostaining in the rat superficial dorsal horn. | the present study determines the effects of sciatic nerve stimulation at intensities that activate a-fibers alone or both a- and c-fibers on immunostaining for substance p (sp), cholecystokinin-octapeptide (cck-8), galanin (gal), dynorphin (dyn) and vasoactive intestinal polypeptide (vip) in the superficial dorsal horn of the rat spinal cord. the goal of this study is to provide a more precise spatial localization of the sites of release or accumulation of these compounds in relation to specific ... | 1992 | 1504792 |
| growth and enzymological characteristics of a pink-pigmented facultative methylotroph methylobacterium sp. mb1. | growth characteristics of batch and continuous cultures of the pink facultative methylotroph methylobacterium sp. mb1 were determined. the response of a chemostat culture to a pulse increase of methanol concentration was studied. malate, succinate and oxaloacetate additions to the methanol-supplemented medium decreased batch culture growth inhibition by methanol. the carotenoid content in cells grown in a chemostat decreased with increasing growth rate. the key enzyme activities of c1-metabolism ... | 1992 | 1505877 |
| determination of maximal oxygen consumption in exercising pregnant sheep. | previous work with pregnant ewes has shown that acute bouts of exercise may cause changes in plasma hormone concentrations, blood flow distribution, and maternal and fetal temperatures. however, most of these studies do not quantify the chosen exercise intensity through measurement of oxygen consumption (vo2). therefore the purpose of this study was to statistically model the vo2 response of pregnant sheep to treadmill (tm) exercise to determine the exercise intensities (% maximal vo2) of previo ... | 1992 | 1506375 |
| identification of regulatory elements within the minimal promoter region of the human endogenous erv9 proviruses: accurate transcription initiation is controlled by an inr-like element. | erv9 is a low repeated family of human endogenous retroviral elements whose expression is mainly detectable in undifferentiated embryonal carcinoma nt2/d1 cells. in this report we have analyzed the minimal promoter region located within the erv9 ltr. using the transient cat expression assay we have identified the minimal promoter region, which includes sequences spanning from -70 to +6 relative to the major transcription start site. deletion analysis, primer extension mapping of the transcriptio ... | 1992 | 1508707 |
| effects of selected inflammatory mediators on blood flow and vascular permeability in the dental pulp. | one of the major events involving inflammatory processes is the alteration of microcirculatory hemodynamics by inflammatory mediators released from tissue components. using modern macrocirculatory techniques, 15 mu radioisotope labeled microspheres, 133xe washout, laser doppler flowmetry and double isotopes, 125 and 131i-albumin, and microcirculatory methods, intravital fluorescence microscopy with fitc labeled dextran, we have examined the effects of selected mediators, e.g. 5-hydroxytryptamine ... | 1992 | 1508895 |
| evidence for an ultraviolet sunscreen role of the extracellular pigment scytonemin in the terrestrial cyanobacterium chlorogloeopsis sp. | the proposed photoprotective role of the uv-a absorbing, extracellular pigment scytonemin was studied in the terrestrial cyanobacterium chlorogloeopsis sp. strain o-89-cgs(1). uv-a (315-400 nm) caused growth delay, cell growth restarting only when scytonemin had accumulated in the extracellular envelopes. cultures with scytonemin were more resistant to photoinhibition of photosynthesis than cultures without scytonemin, the differential resistance being much greater to uv-a-caused photoinhibition ... | 1992 | 1508978 |
| [comparative epidemiological characteristics of a homogeneous series of acute common pneumopathies seen in a general hospital center (ghc)]. | between 1979 and 1989, 108 cases of community acquired pneumonia in non-immunodepressed subjects were admitted to hospital to a specialist chest department. the patient's characteristics were as follows: mean age = 56 (range 14-91), 44 women and 64 men. the causal agent was isolated in 44 cases (40%) of which 4 cases were viral (9% of 44 cases) and 12 were legionella (27%), pneumococci (sp) 11 cases (25%), mycoplasma pneumoniae (mp) 8 cases (18%), and chlamydia 8 cases. the microbacteriological ... | 1992 | 1509189 |
| epidemiology of plasmid-mediated beta-lactamases in enterobacteria swedish neonatal wards and relation to antimicrobial therapy. | tem-1, oxa-1, shv-1, and related beta-lactamases in fecal isolates from 953 infants in 22 swedish neonatal intensive care units were studied by dna hybridization. tem-1- and oxa-1-positive isolates were always escherichia coli and represented 86 and 8%, respectively, of the ampicillin-resistant isolates of this species. shv-1 was found in 16% of the klebsiella sp. (mainly klebsiella pneumoniae) isolates. tem-1 and shv-1 occurred in 14 and 16 units and in up to 64 and 26% of the neonates, respect ... | 1992 | 1510425 |
| synthesis in vitro of very long chain fatty acids in vibrio sp. strain abe-1. | the activity of fatty acid synthetase (fas) from vibrio sp. strain abe-1 required the presence of acyl carrier protein and was completely inhibited by thiolactomycin, an inhibitor specific for a type ii fas. these observations indicate that this enzyme is a type ii fas. analysis by gas-liquid chromatography of the reaction products synthesized in vitro from [2-14c]malonyl-coa by the partially purified fas revealed, in addition to 16- and 18-carbon fatty acids which are normal constituents of thi ... | 1992 | 1510554 |
| clostridium neopropionicum sp. nov., a strict anaerobic bacterium fermenting ethanol to propionate through acrylate pathway. | strain x4 was isolated several years ago from an anaerobic mesophilic plant treating vegetable cannery waste waters. it was the first example of propionic fermentation from ethanol. morphologic and physiologic characterizations of the strain are presented here. this strain is described as type strain of a new species, clostridium neopropionicum sp. nov. whole cells of strain x4 ferment [1-13c] ethanol and co2 to [2-13c] propionate, [1-13c] acetate and [2-13c] propanol, suggesting the absence of ... | 1992 | 1510558 |
| environmental regulation of alcohol metabolism in thermotolerant methylotrophic bacillus strains. | the thermotolerant methylotroph bacillus sp. c1 possesses a novel nad-dependent methanol dehydrogenase (mdh), with distinct structural and mechanistic properties. during growth on methanol and ethanol, mdh was responsible for the oxidation of both these substrates. mdh activity in cells grown on methanol or glucose was inversely related to the growth rate. highest activity levels were observed in cells grown on the c1-substrates methanol and formaldehyde. the affinity of mdh for alcohol substrat ... | 1992 | 1510560 |
| creatinine and n-methylhydantoin degradation in two newly isolated clostridium species. | with n-methylhydantoin (nmh) as the main organic substrate, two strictly anaerobic spore forming gram-positive bacterial strains were isolated from sewage sludge. these strains, named clostridium sp. fs23 and clostridium sp. fs41, totally degraded nmh, via n-carbamoylsarcosine (cs) and sarcosine as intermediates. strain fs23 grew also with creatinine, which was converted to nmh by creatinine iminohydrolase (ec 3.5.4.21). this enzyme was formed at high rates with all substrates tested. cytosine a ... | 1992 | 1510564 |
| oxygen yield and thermoluminescence characteristics of a cyanobacterium lacking the manganese-stabilizing protein of photosystem ii. | previous experiments have shown that a synechocystis sp. pcc 6803 mutant (delta psbo) lacking the extrinsic manganese-stabilizing protein (msp) exhibits impaired, but significant levels of h2o-splitting activity [burnap, r., & sherman, l.a. (1991) biochemistry 30, 440-446]. [14c]dcmu-binding experiments now show that the number and affinity of dcmu-binding sites (normalized to chlorophyll) are equivalent in delta psbo and the wild type, suggesting equal concentrations of assembled reaction cente ... | 1992 | 1510930 |
| the complete nucleotide sequence of the gene (rpod1) encoding the principal sigma factor of the rna polymerase from the cyanobacterium synechococcus sp. strain pcc7942. | the complete nucleotide sequence of rpod1 gene from synechococcus pcc7942 has been determined. the nucleotide data have indicated the presence of an open reading frame of 1155 base pairs encoding a polypeptide which shares the framework structure for principal sigma factors of eubacterial strains. | 1992 | 1511015 |
| a role for acetylcholine in conditioning-related responses of rat frontal cortex neurons: microiontophoretic evidence. | in an associative conditioning paradigm, an auditory stimulus (cs+) was paired with rewarding medial forebrain bundle stimulation or a tone of different frequency (cs-) was presented without pairing. after training, slow potential (sp) and single neuron responses were recorded from rat frontal cortex. when cortical sp responses indicated the development of discrimination between cs+ and cs- tones, single neurons could be isolated that exhibited a discriminative response to cs+. seventy-three per ... | 1992 | 1511348 |
| stereoselectivity of lipases: esterification reactions of octadecylglycerol. | stereoselectivity of several triacylglycerol lipases (ec 3.1.1.3) has been investigated in the enzymatic esterification of rac-1-o-octadecylglycerol with oleic acid in the presence of organic solvents, such as hexane. x-1(3)-o-octadecylmonooleoylglycerols were the only products formed with most lipases; considerable proportions of x-1(3)-o-octadecyldioleoylglycerols were also formed with the lipase from candida cylindracea. the mixtures of unesterified enantiomeric substrates, i.e., x-1(3)-o-oct ... | 1992 | 1511492 |
| antinociception induced by intracerebroventricular or intrathecal administration of gentamicin in rats. | 1. the intrathecal (i.t.) administration of gentamicin (ad50 = 3.98 micrograms) produced marked and dose-dependent antinociception in rats, as assessed by the tail-flick test. a weaker effect was obtained following intracerebroventricular drug administration (ad50 = 50.12 micrograms). 2. a transitory but dose-dependent antagonism of the effect of i.t. gentamicin was obtained following i.t. administration of calcium chloride (4 and 8 micrograms). 3. the magnitude and duration of morphine antinoci ... | 1992 | 1511855 |
| immunohistochemical identification of penicillium marneffei by monoclonal antibody. | we present an experimental study on the immunohistochemical identification of penicillium marneffei in paraffin-embedded, formalin-fixed tissue. the monoclonal antibody eb-a1 detects a specific galactomannan that appears to have at least one epitope identical in p. marneffei and aspergillus sp. this immunohistochemical approach could be useful in the diagnosis of a rare diseae, penicilliosis marneffei, which proves to be difficult to identify by conventional microscopy. | 1992 | 1512093 |
| confirmation of oxidative dehalogenation of pentachlorophenol by a flavobacterium pentachlorophenol hydroxylase. | pentachlorophenol (pcp) hydroxylase purified from flavobacterium sp. strain atcc 39723 converted pcp or 2,3,5,6-tetrachlorophenol to tetrachloro-p-hydroquinone (tech) with the co-consumption of o2 and nadph. the purified enzyme incorporated 18o from 18o2 but not from h218o into the reaction end product tech. the results clearly demonstrate that pcp is oxidatively converted to tech by a monooxygenase-type enzyme from flavobacterium sp. strain atcc 39723. | 1992 | 1512208 |
| lack of homology between two haloacetate dehalogenase genes encoded on a plasmid from moraxella sp. strain b. | two genes encoding haloacetate dehalogenases, h-1 and h-2, are closely linked on a plasmid from moraxella sp. strain b. h-1 predominantly acts on fluoroacetate, but h-2 does not. to elucidate the molecular relationship between the two enzymes, we compared their structural genes. two restriction fragments of the plasmid dna were subcloned on m13 phages and their nucleotide sequences were determined. the sequence of each fragment contained an open reading frame that was identified as the structura ... | 1992 | 1512562 |
| molecular cloning and characterization of an alkalophilic bacillus sp. c125 gene homologous to bacillus subtilis sec y. | a 1.8 kb hindiii dna fragment containing the secy gene of alkalophilic bacillus sp. c125 has been cloned into plasmid puc119 using the b. subtilis secy gene as a probe. the complete nucleotide sequence of the cloned dna indicated that it contained one complete orf and parts of two other orfs. the similarity of these orfs to the sequences of the b. subtilis proteins indicated that they were the genes for ribosomal protein l15-secy-adenylate kinase, in that order. the gene product of the alkalophi ... | 1992 | 1512566 |
| borrelia sp. in ticks recovered from white-tailed deer in alabama. | six hundred sixty-five hunter-killed white-tailed deer (odocoileus virginianus) from 18 counties in alabama (usa) were examined for ticks. most of the collections were made at state-operated wildlife management areas. four species of ticks (n = 4,527) were recovered: the lone star tick amblyomma americanum (n = 482); the gulf coast tick a. maculatum (n = 11); the winter tick dermacentor albipictus (n = 1,242); and the black-legged tick ixodes scapularis (n = 2,792). fifty-six percent of the tick ... | 1992 | 1512879 |
| [pharmacokinetic, bacteriological, and clinical studies on panipenem/betamipron in children]. | pharmacokinetic, bacteriological and clinical studies were performed on panipenem/betamipron (papm/bp) in children. the results are summarized as follow: 1. twelve patients with various bacterial infectious diseases were treated with papm/bp. each dose was 20 mg/20 mg/kg, administered 3 times daily, in 30-minute intravenous drip infusion. treatments were continued for 5-22 days. clinical efficacies of papm/bp in 12 patients with bacterial infections (1 with suspected sepsis, 5 with pneumonia, 1 ... | 1992 | 1512926 |