| first identification of cryptosporidium parvum subtype iiaa20g1r1 in water buffalos (bubalus bubalis). | cryptosporidium can infect a wide variety of vertebrate animals, including mammals, birds, amphibians, reptiles, and fish. there are few molecular characterizations of cryptosporidium isolated from water buffalo. thus, the present study investigated the occurrence and molecular characterization of cryptosporidium spp. in water buffalos by nested-pcr. non-diarrheic feces were obtained from 122 water buffalo calves. all samples were tested by nested-pcr based on the 18s rrna gene, after which posi ... | 2018 | 29514125 |
| selection of suitable reference genes for normalization of quantitative rt-pcr (rt-qpcr) expression data across twelve tissues of riverine buffaloes (bubalus bubalis). | selection of reference genes has become an integral step in any real time quantitative pcr (rt-qpcr) based expression studies. the importance of this study stems from the fact that riverine buffaloes are major dairy species of indian sub-continent and the information generated here will be of great interest to the investigators engaged in functional genomic studies of this important livestock species. in this study, an effort was made to evaluate a panel of 10 candidate reference genes (glyceral ... | 2018 | 29509770 |
| comparative sequence alignment reveals river buffalo genomic structural differences compared with cattle. | this study sought to characterize differences in gene content, regulation and structure between taurine cattle and river buffalo (one subspecies of domestic water buffalo) using the extensively annotated umd3.1 cattle reference genome as a basis for comparisons. we identified 127 deletion cnv regions in river buffalo representing 5 annotated cattle genes. we also characterized 583 merged mobile element insertion (mei) events within the upstream regions of annotated cattle genes. transcriptome an ... | 2019 | 29501677 |
| effect of consecutive re-synchronization protocols on pregnancy rate in buffalo (bubalus bubalis) heifers out of the breeding season. | the combined effect of six consecutive timed artificial inseminations (tais) on pregnancy rates, following two different synchronization protocols on buffalo heifers, over a period of seven months typically characterized by low breeding performances, were investigated in this study. a total of 2189 tais were performed on 1463 buffalo heifers within a large buffalo farm in the south of italy. individual animals were allowed to undergo synchronization protocol (either a slightly modified ovsynch o ... | 2018 | 29494837 |
| hepatobiliary diseases in buffalo (bubalus bubalis): clinical, laboratory, and ultrasonographic findings. | this study describes ultrasonographic observations of five hepatobiliary diseases in buffalo (bubalus bubalis). fifty buffalo, including 20 clinically normal and 30 hepatobiliary diseased buffalo were enrolled in the study. complete clinical, radiographic and ultrasonographic examinations and laboratory analyses were conducted. focal parenchymal lesions including liver abscess (n = 12) and hepatic cyst (n = 6), diffuse parenchymal lesion (hepatobiliary cirrhosis, n = 5) and obstruction of hepato ... | 2018 | 29486538 |
| genome-wide gene expression analysis of 45 days pregnant fetal cotyledons vis-a-vis non-pregnant caruncles in buffalo (bubalus bubalis). | the crosstalk between fetus and mother starts with the onset of placental attachment to the uterus. the cotyledons and caruncles are the two anatomically distinct structures that play a crucial role in this physiological communication. using agilent gene chip genome microarray, we measured the expression profile of pregnancy cotyledons in comparison to caruncular reminiscence of the uteri in non-pregnant buffalo (bubalus bubalis) for the detection of the early post-pregnancy rapid changes in cel ... | 2018 | 29454089 |
| establishment, growth, proliferation, and gene expression of buffalo (bubalus bubalis) transgenic fetal fibroblasts containing human insulin gene, and production of embryos by handmade cloning using these cells. | the aim of the present study was to compare transgenic cells, containing human insulin gene kept under the control of mammary gland-specific buffalo beta-lactoglobulin promoter, and their counterparts, that is, nontransgenic cells, for examining their potential for the production of embryos following somatic cell nuclear transfer (scnt). the gene construct was delivered into buffalo fetal fibroblasts (bff) by nucleofection following which, the transfected cells were selected by culture in the pr ... | 2018 | 29446977 |
| epigenetic alteration of donor cells with histone deacetylase inhibitor m-carboxycinnamic acid bishydroxymide improves the in vitro developmental competence of buffalo (bubalus bubalis) cloned embryos. | epigenetic reprogramming is an indispensable process during the course of mammalian development, but aberrant in cloned embryos. the aim of this study was to examine the effect of donor cell treatment with histone deacetylase (hdac) inhibitor m-carboxycinnamic acid bishydroxymide (cbha) on cloned embryo development and establish its optimal concentration. different concentrations of cbha (2.5, 5.0, 10.0, and 20.0 μm) were used to treat buffalo adult fibroblast cells for 24 hours and effect on ce ... | 2018 | 29412736 |
| hemotropic mycoplasmas infection in water buffaloes (bubalus bubalis) from northeastern brazil. | two species of hemotropic mycoplasmas (hm) are known to infect large domestic ruminants, mycoplasma wenyonii and 'candidatus mycoplasma haemobos'. although hm has been described in cattle worldwide, data in water buffaloes (bubalus bubalis) remain scarce. accordingly, the aim was to determine the occurrence of hm in water buffaloes from northeastern brazil. a total of 101/290 (34.83%) buffaloes were positive for hm (16 m. wenyonii alone, 6 'ca. m. haemobos' alone and 79 both). this was the first ... | 2018 | 29406279 |
| morphological and molecular identification of explanatum explanatum in domestic water buffalo in pakistan. | more than 70 species of the family paramphistomatidae, have been identified in ruminants in different parts of the world. most are pathogenic, causing amphistomosis. adult flukes of this family have a predilection for the rumen, liver or bile duct of ruminants where they may cause damage to the epithelium. identification of adult paramphistomes to the species level based on morphology alone requires specialized knowledge, whereas, molecular genetic marker analysis is more precise and transferabl ... | 2017 | 31014638 |
| modulation of immunity and inflammatory gene expression in buffalo (bubalus bubalis) with some digestive disorders. | so far, there has been scarce information about the status of immunoglobulins (ig) and the gene expression of inflammatory cytokines in buffaloes showing digestive troubles. the purpose of the present study was to explore the modulation of gene expression of some immune-inflammatory markers in buffaloes suffered from various digestive disorders. for this reason, 50 native breed water buffaloes were studied. forty of these buffaloes showed various symptoms of digestive disorders and were allocate ... | 2017 | 32214977 |
| clinical, laboratory and ultrasonographic findings in egyptian buffalo (bubalus bubalis) with caecal and colonic dilatation. | the present study was conducted to describe the clinical, laboratory and ultrasonographic findings of caecal and colonic dilatation in egyptian buffalo (bubalus bubalis). a total number of forty buffaloes were included in the study and divided into two groups: control group (n = 20) and diseased group (n = 20). diseased buffalo were admitted to the veterinary teaching hospital at assiut university-egypt. each of the diseased animals was subjected to clinical, rectal, laboratory and ultrasonograp ... | 2016 | 30255032 |
| characterization of buffalo interleukin 8 (il-8) and its expression in endometritis. | river buffalo (bubalus bubalis bubalis) with a population over 135 million heads is an important livestock. interleukin 8 (il-8) is a member of the chemokine family and is an important chemoattractant for neutrophils associated with a wide variety of inflammatory diseases such as endometritis. tissue samples from the mammary gland, uterus and ovary were obtained from river buffalo (mediterranean type) with and without endometritis. bacteriological examination showed the presence of both gram pos ... | 2015 | 30647569 |
| structural analysis of covid-19 spike protein in recognizing the ace2 receptor of different mammalian species and its susceptibility to viral infection. | the pandemic covid-19 was caused by a novel coronavirus-2 (sars-cov-2) that infects humans through the binding of glycosylated sars-cov-2 spike 2 protein to the glycosylated ace2 receptor. the spike 2 protein recognizes the n-terminal helices of the glycosylated metalloprotease domain in the human ace2 receptor. to understand the susceptibility of animals for infection and transmission, we did sequence and structure-based molecular interaction analysis of 16 ace2 receptors from different mammali ... | 2021 | 33552834 |
| viral infections shared between water buffaloes and small ruminants in switzerland. | importation of exotic animals that may harbor infectious agents poses risks for native species with potentially severe impacts on animal health and animal production. although the asian water buffalo (bubalus bubalis) population in europe is steadily increasing, its susceptibility to viral infections and its role for interspecies transmission is largely unknown. to identify viral infections that are shared between exotic water buffaloes and native small ruminants, we collected blood samples from ... | 2021 | 34166139 |
| evaluation of safety and efficacy of an inactivated marker vaccine against bovine alphaherpesvirus 1 (bohv-1) in water buffalo (bubalus bubalis). | recent studies have explored the seropositivity of bovine alphaherpesvirus 1 (bohv-1) in water buffaloes, suggesting the urgency for developing strategies to eradicate the virus involving both cattle and water buffaloes. however, in europe, the glycoprotein e (ge) deleted marker vaccines against bohv-1 are commercially available only for the cattle industry. this study, for the first time, evaluated the safety and efficacy of a commercial inactivated ge-deleted marker vaccine in water buffalo. f ... | 2021 | 33917160 |
| characterization of foot and mouth disease virus serotype sat-2 in swamp water buffaloes (bubalus bubalis) under the egyptian smallholder production system. | spontaneous mutations are a common characteristic of the foot and mouth disease virus (fmdv), leading to wide antigenic variations resulting in the emergence of new topotypes and lineages of fmdv, which contributes to occasional vaccination failures. the objectives of the present study were to genetically characterize fmdv isolated from water buffaloes and study the biochemical and histopathological indicators of infected animals. fifty-four water buffaloes of both sexes and different ages suffe ... | 2021 | 34200281 |
| prevalence of foot and mouth disease virus in apparently healthy buffaloes brought to islamabad slaughterhouse in pakistan. | this study was conducted to determine the sero-prevalence of foot and mouth disease (fmd) in buffaloes (bubalus bubalis) slaughtered at islamabad slaughterhouse, pakistan. sera and oro-pharyngeal (op) fluid were collected from 630 healthy buffaloes. these animals originated from various livestock markets from 14 districts of punjab. the age of animals ranged from 1 to 15 years. sera were analyzed using fmd virus (fmdv) nonstructural proteins (nsp) and indirect elisa. the sero-prevalence of fmd w ... | 2018 | 33601788 |