| involvement of quinolinate phosphoribosyl transferase in promotion of potato growth by a burkholderia strain. | burkholderia sp. strain psjn stimulates root growth of potato explants compared to uninoculated controls under gnotobiotic conditions. in order to determine the mechanism by which this growth stimulation occurs, we used tn5 mutagenesis to produce a mutant, h41, which exhibited no growth-promoting activity but was able to colonize potato plants as well as the wild-type strain. the gene associated with the loss of growth promotion in h41 was shown to exhibit 65% identity at the amino acid level to ... | 2006 | 16391116 |
| melioidotic osteomyelitis treated with antibiotic-calcium hydroxyapatite composite: case report with four-year follow-up. | melioidosis is caused by an infection by burkholderia pseudomallei. osteomyelitis is a recognised manifestation of melioidosis but burkholderia pseudomallei is a relatively rare aetiological agent in musculoskeletal infections. we report a 32-year-old diabetic man with septicaemia due to melioidotic infection of the spleen, liver and distal femur. the osteomyelitis relapsed despite being treated with the standard radical debridement and insertion of gentamycinimpregnated polymethylmetacrylate (p ... | 2006 | 16397726 |
| effect of ph on the toxicity of nickel and other divalent metals to burkholderia cepacia pr1(301). | nickel (ni) is a common cocontaminant at many waste sites where the soils and sediments often are acidic, thereby influencing metal availability. growth of burkholderia cepacia pr1(301) was not affected at 3.41 mm ni at ph 5, but was inhibited by 73.2% at ph 6 and inhibited completely at ph 7 compared to growth without ni. this ph effect was not observed in the ni-resistant strains, ralstonia metallidurans ch34 and 31a. predicted ni speciation did not explain the observed toxicity trends. sorpti ... | 2005 | 16398108 |
| rflp analysis of the rrna operon of a brazilian collection of bradyrhizobial strains from 33 legume species. | genetic diversity in tropical rhizobial species is still poorly known. with the aim of increasing this knowledge, three ribosomal regions of 119 strains belonging to the official brazilian culture collection of rhizobia and classified as bradyrhizobium based on morphological and physiological characteristics in vitro were analysed by rflp-pcr. the strains were isolated from 33 legume species, representing nine tribes and all three subfamilies; they all form very effective n2-fixing nodules and 4 ... | 2006 | 16403890 |
| microbiology of airway disease in a cohort of patients with cystic fibrosis. | recent reports document an increasing incidence of new gram-negative pathogens such as stenotrophomonas maltophilia and alcaligenes xylosoxidans isolated from patients with cystic fibrosis, along with an increase in common gram-negative pathogens such as pseudomonas aeruginosa and burkholderia cepacia complex. furthermore, the increase in multidrug-resistance of such organisms makes the therapeutic management of these patients more problematic. therefore, careful isolation and identification, an ... | 2006 | 16405721 |
| simultaneous coinfection by multiple strains during burkholderia cepacia complex infection in cystic fibrosis. | much remains unknown about the natural history of respiratory tract infection by burkholderia cepacia complex (bcc) in persons with cystic fibrosis (cf). specifically, it is not clear whether infected cf patients typically harbor a single bcc strain or multiple strains that may be phenotypically indistinguishable. we genotyped 912 bcc isolates recovered from cf sputum culture from in excess of 100 patients to demonstrate that chronic infection generally involves a single strain. transient coinfe ... | 2006 | 16406186 |
| molecular detection of catabolic genes for polycyclic aromatic hydrocarbons in the reed rhizosphere of sunchon bay. | this study focused on detecting catabolic genes for polycyclic aromatic hydrocarbons (pahs) distributed in the reed rhizosphere of sunchon bay, korea. these marsh and mud environments were severely affected by human activities, including agriculture and fisheries. our previous study on microbial roles in natural decontamination displayed the possibility that pah-degrading bacteria, such as achromobacter sp., alcaligenes sp., burkholderia sp. and pseudomonas sp. play an important decontamination ... | 2005 | 16410776 |
| treatment of cork boiling wastewater using chemical oxidation and biodegradation. | three cultures were enriched from cork boiling wastewater using tannic acid as the selective carbon substrate, at 25 degrees c and ph 7.2, 25 degrees c and ph 4.7 and 50 degrees c and ph 4.7. the enrichment culture obtained at neutral ph was composed of five culturable isolates, whereas from each acidic enrichment two bacterial strains were isolated. mesophilic isolates were gram negative bacteria belonging to the genera klebsiella, pseudomonas, stenotrophomonas and burkholderia. thermophilic is ... | 2006 | 16414098 |
| daytime orthokeratology associated with infectious keratitis by multiple gram-negative bacilli: burkholderia cepacia, pseudomonas putida, and pseudomonas aeruginosa. | to report a case of a corneal ulcer in a patient who wore orthokeratology contact lenses during the day. | 2006 | 16415688 |
| actin-dependent movement of bacterial pathogens. | listeria, rickettsia, burkholderia, shigella and mycobacterium species subvert cellular actin dynamics to facilitate their movement within the host cytosol and to infect neighbouring cells while evading host immune surveillance and promoting their intracellular survival. 'attaching and effacing' escherichia coli do not enter host cells but attach intimately to the cell surface, inducing motile actin-rich pedestals, the function of which is currently unclear. the molecular basis of actin-based mo ... | 2006 | 16415925 |
| production of (r)-3-amino-3-phenylpropionic acid and (s)-3-amino-3-phenylpropionic acid from (r,s)-n-acetyl-3-amino-3-phenylpropionic acid using microorganisms having enantiomer-specific amidohydrolyzing activity. | (r)-3-amino-3-phenylpropionic acid ((r)-beta-phe) and (s)-3-amino-3-phenylpropionic acid ((s)-beta-phe) are key compounds on account of their use as intermediates in synthesizing pharmaceuticals. enantiomerically pure non-natural amino acids are generally prepared by enzymatic resolution of the racemic n-acetyl form, but despite the intense efforts this method could not be used for preparing enantiomerically pure beta-phe, because the effective enzyme had not been found. therefore, screening for ... | 2006 | 16428826 |
| identical burkholderia cepacia complex strain types isolated from multiple patients attending a hospital in brazil. | | 2006 | 16434721 |
| attempted passive prophylaxis with a monoclonal anti-burkholderia pseudomallei exopolysaccharide antibody in a murine model of melioidosis. | melioidosis is a severe gram-negative infection caused by the facultative intracellular bacterium burkholderia pseudomallei, which is responsible for a broad spectrum of symptoms in both humans and animals. no licensed vaccine currently exists. this study evaluated the protective effect of a monoclonal antibody (mab ps6f6) specific to b. pseudomallei exopolysaccharide in an outbred murine model of sub-acute melioidosis. when administered before the infectious challenge, ps6f6 significantly incre ... | 2005 | 16435577 |
| in vitro activity of doripenem against pseudomonas aeruginosa and burkholderia cepacia isolates from both cystic fibrosis and non-cystic fibrosis patients. | the in vitro activities of doripenem, imipenem, levofloxacin, piperacillin, ceftazidime, aztreonam, tobramycin, and cefepime were determined for 160 isolates of pseudomonas aeruginosa (82 from cystic fibrosis [cf] patients) and 34 isolates of burkholderia cepacia. doripenem mic90s were lower than those of all other comparative agents against all isolates combined and against all p. aeruginosa isolates. doripenem was as active as levofloxacin and 2- to 32-fold more active than the other comparati ... | 2006 | 16436756 |
| [virulent properties of hospital strains of bacteria of the burkholderia cepacia complex, isolated in hospitals of moscow]. | the results of the study of hospital strains of the b. cepacia complex, isolated in hospitals of moscow, with the use of phenotypical and molecular-genetic methods are presented. the phenotypical methods made it possible to differentiate russian strains and classify them with a group of genomovars (i, iii, iv). as the result the epidemic importance of the strains with epidemic markers, having specific characteristics for every clinic, was determined. the detection of the collection of genes cepi ... | 2005 | 16438375 |
| molecular phylogeny based on the 16s rrna gene of elite rhizobial strains used in brazilian commercial inoculants. | nitrogen is often a limiting nutrient, therefore the sustainability of food crops, forages and green manure legumes is mainly associated with their ability to establish symbiotic associations with stem and root-nodulating n2-fixing rhizobia. the selection, identification and maintenance of elite strains for each host are critical. decades of research in brazil resulted in a list of strains officially recommended for several legumes, but their genetic diversity is poorly known. this study aimed a ... | 2006 | 16442259 |
| development of a 5'-nuclease real-time pcr assay targeting flip for the rapid identification of burkholderia mallei in clinical samples. | burkholderia mallei is a potential biological agent that causes glanders or farcy in solipeds, a disease notifiable to the office international des epizooties (oie). the number of reported outbreaks has increased steadily during the last decade, but diagnosis is hampered by the low bacterial load in infected tissues and excretions. | 2006 | 16449212 |
| burkholderia terrae sp. nov., isolated from a forest soil. | a gram-negative, slightly curved rod-shaped bacterium, designated strain kmy02t, was isolated from a forest soil in daejeon, south korea. on the basis of 16s rrna gene sequence similarity, strain kmy02t was shown to belong to the family burkholderiaceae of the betaproteobacteria, and to be related most closely to burkholderia hospita lmg 20598t (98.7%), burkholderia caribensis lmg 18531t (98.0%) and burkholderia phymatum lmg 21445t (97.4%). its phylogenetic distance from all recognized species w ... | 2006 | 16449457 |
| isolation and identification of natural endophytic rhizobia from rice (oryza sativa l.) through rdna pcr-rflp and sequence analysis. | three novel endophytic rhizobial strains (rre3, rre5, and rre6) were isolated from naturally growing surface sterilized rice roots. these isolates had the ability to nodulate common bean (phaseolus vulgaris). polymerase chain reaction-restriction fragment length polymorphism and sequencing of 16s rdna of these isolates revealed that rre3 and rre5 are phylogenetically very close to burkholderia cepacia complex, whereas rre6 has affinity with rhizobium leguminosarum bv. phaseoli. plant infection t ... | 2006 | 16450073 |
| parabs systems of the four replicons of burkholderia cenocepacia: new chromosome centromeres confer partition specificity. | most bacterial chromosomes carry an analogue of the parabs systems that govern plasmid partition, but their role in chromosome partition is ambiguous. parabs systems might be particularly important for orderly segregation of multipartite genomes, where their role may thus be easier to evaluate. we have characterized parabs systems in burkholderia cenocepacia, whose genome comprises three chromosomes and one low-copy-number plasmid. a single parab locus and a set of parb-binding (pars) centromere ... | 2006 | 16452432 |
| genetic and transcriptional analysis of the siderophore malleobactin biosynthesis and transport genes in the human pathogen burkholderia pseudomallei k96243. | burkholderia pseudomallei is a gram-negative facultative intracellular pathogen that causes melioidosis, an invasive disease of humans and animals. to address the response of this bacterium to iron-limiting conditions, we first performed a global transcriptional analysis of rna extracted from bacteria grown under iron-limiting and iron-rich conditions by microarrays. we focused our study on those open reading frames (orfs) induced under iron limitation, which encoded predicted proteins that coul ... | 2006 | 16452439 |
| phenotypic characterization of raw milk-associated psychrotrophic bacteria. | among the 68 isolates, selected from 13 raw-milk samples in finland (that originate from farm, truck or silo tanks), 60 (88%) were psychrotrophs. all the isolates were characterized by the determination of their spoilage and phenotypic features: proteolytic and lipolytic activities, the production of lecithinases and hemolytic factors were considered. phenotypic characterization of the isolates was mainly performed with api 20ne and biolog gn2 identification systems; the results were system-depe ... | 2006 | 16459065 |
| biodegradation of 3-nitrotyrosine by burkholderia sp. strain js165 and variovorax paradoxus js171. | the cascade of reactive nitrogen species generated from nitric oxide causes modification of proteins, lipids, and nucleic acids in a wide range of organisms. 3-nitrotyrosine is one of the most common products of the action of reactive nitrogen species on proteins. although a great deal is known about the formation of 3-nitrotyrosine, the subsequent metabolism of this compound is a mystery. variovorax paradoxus js171 and burkholderia sp. strain js165 were isolated from soil slurries when 3-nitrot ... | 2006 | 16461647 |
| coexistence of burkholderia, cupriavidus, and rhizobium sp. nodule bacteria on two mimosa spp. in costa rica. | rrna gene sequencing and pcr assays indicated that 215 isolates of root nodule bacteria from two mimosa species at three sites in costa rica belonged to the genera burkholderia, cupriavidus, and rhizobium. this is the first report of cupriavidus sp. nodule symbionts for mimosa populations within their native geographic range in the neotropics. burkholderia spp. predominated among samples from mimosa pigra (86% of isolates), while there was a more even distribution of cupriavidus, burkholderia, a ... | 2006 | 16461667 |
| root-associated bacteria contribute to mineral weathering and to mineral nutrition in trees: a budgeting analysis. | the principal nutrient source for forest trees derives from the weathering of soil minerals which results from water circulation and from plant and microbial activity. the main objectives of this work were to quantify the respective effects of plant- and root-associated bacteria on mineral weathering and their consequences on tree seedling growth and nutrition. that is why we carried out two column experiments with a quartz-biotite substrate. the columns were planted with or without pine seedlin ... | 2006 | 16461674 |
| a quorum-quenching approach to investigate the conservation of quorum-sensing-regulated functions within the burkholderia cepacia complex. | taxonomic studies of the past few years have shown that the burkholderia cepacia complex, a heterogeneous group of b. cepacia-like organisms, consists of at least nine species. b. cepacia complex strains are ubiquitously distributed in nature and have been used for biocontrol, bioremediation, and plant growth promotion purposes. at the same time, b. cepacia complex strains have emerged as important opportunistic pathogens of humans, particularly those with cystic fibrosis. all b. cepacia complex ... | 2006 | 16461713 |
| purification, characterization, and molecular cloning of organic-solvent-tolerant cholesterol esterase from cyclohexane-tolerant burkholderia cepacia strain st-200. | extracellular cholesterol esterase of burkholderia cepacia strain st-200 was purified from the culture supernatant. its molecular mass was 37 kda. the enzyme was stable at ph 5.5-12 and active at ph 5.5-6, showing optimal activity at ph 7.0 at 45 degrees c. relative to the commercially available cholesterol esterases, the purified enzyme was highly stable in the presence of various water-miscible organic solvents. the enzyme preferentially hydrolyzed long-chain fatty acid esters of cholesterol, ... | 2006 | 16463077 |
| new temperate dna phage bcp15 acts as a drug resistance vector. | this study was designed to determine the role of a new temperate dna phage bcp15 in relation to drug resistance. the multidrug resistant shigella flexneri nk1925 was isolated from a patient of infectious diseases hospital, kolkata, india. this strain contained five plasmids ranging in size from 3 to 212 kb. after curing of five plasmids, this strain became sensitive to antibiotics. a plasmidless multidrug-resistant strain burkholderia cepacia dr11 was isolated during the survey of microorganisms ... | 2006 | 16463125 |
| detection of the reemerging agent burkholderia mallei in a recent outbreak of glanders in the united arab emirates by a newly developed flip-based polymerase chain reaction assay. | a polymerase chain reaction (pcr) assay targeting the flagellin p (flip)-i s407a genomic region of burkholderia mallei was developed for the specific detection of this organism in pure cultures and clinical samples from a recent outbreak of equine glanders. primers deduced from the known flip-is407a sequence of b. mallei american type culture collection (atcc) 23344(t) allowed the specific amplification of a 989-bp fragment from each of the 20 b. mallei strains investigated, whereas other closel ... | 2006 | 16466896 |
| indirect hemagglutination assay in patients with melioidosis in northern australia. | melioidosis is caused by the saprophytic organism burkholderia pseudomallei. the use of the indirect hemagglutination assay (iha) has found widespread use in areas endemic for this disease. using this assay, we explored the serologic profile of 275 patients with culture-confirmed melioidosis in the northern territory of australia. based on a threshold titer of 1:40, the sensitivity of the iha on admission was 56%. female patients, those with positive blood cultures, and those with pneumonia inde ... | 2006 | 16474092 |
| influence of a nonaqueous phase liquid (napl) on biodegradation of phenanthrene. | a series of batch reactor experiments was carried out to examine the effect of a nonaqueous phase liquid (napl) on the biodegradation of a hydrophobic solute. a mathematical program model that describes physical processes of solute solubilization and partitioning between the napl and aqueous phases as well as microbial degradation and oxygen utilization was used to analyze the test data. the model calculates the cumulative changes in concentration of substrate, cell mass, carbon dioxide, and dis ... | 2006 | 16477359 |
| the clc element of pseudomonas sp. strain b13, a genomic island with various catabolic properties. | pseudomonas sp. strain b13 is a bacterium known to degrade chloroaromatic compounds. the properties to use 3- and 4-chlorocatechol are determined by a self-transferable dna element, the clc element, which normally resides at two locations in the cell's chromosome. here we report the complete nucleotide sequence of the clc element, demonstrating the unique catabolic properties while showing its relatedness to genomic islands and integrative and conjugative elements rather than to other known cata ... | 2006 | 16484212 |
| phenylacetylene reversibly inhibits the phenol hydroxylase of pseudomonas sp. cf600 at high concentrations but is oxidized at lower concentrations. | alkynes are mechanism-based inhibitors of several bacterial monooxygenases, including the soluble methane monooxygenase (smmo) of methylococcus capsulatus and the toluene o-monooxygenase (tom) of burkholderia cepacia g4. in this paper, we investigated the inhibition of the phenol hydroxylase of pseudomonas sp. cf600 by the alkyne phenylacetylene. growth of cf600 on phenol and phenol hydroxylase activity were inhibited by phenylacetylene concentrations greater than 1.0 mm. unlike other alkynes, w ... | 2006 | 16485115 |
| a resazurin-based biosensor for organic pollutants. | a new rapid biosensor method employing the dye resazurin as an indicator of bacterial respiration has been developed to provide a rapid, facile and specific biosensor for environmental contaminants that does not rely on genetic modification techniques, is suitable for a high-throughput multiwell format, and is ideally suited to resource-constrained environmental monitoring situations. this whole-cell biosensor has been applied to the test analyte toluene using natural toluene-degrading bacteria ... | 2006 | 16487702 |
| quorum sensing in the genus burkholderia. | the genus burkholderia contains over 30 species, many of which are important human pathogens. in addition to the primary pathogens burkholderia pseudomallei and burkholderia mallei, several species have emerged as opportunistic pathogens in persons suffering from cystic fibrosis (cf) and immunocompromised individuals. all burkholderia species investigated so far employ quorum-sensing (qs) systems that rely on n-acyl-homoserine lactone (ahl) signal molecules to express certain phenotypic traits i ... | 2006 | 16490397 |
| degradation of phenanthrene by burkholderia sp. c3: initial 1,2- and 3,4-dioxygenation and meta- and ortho-cleavage of naphthalene-1,2-diol. | burkholderia sp. c3 was isolated from a polycyclic aromatic hydrocarbon (pah)-contaminated site in hilo, hawaii, usa, and studied for its degradation of phenanthrene as a sole carbon source. the initial 3,4-c dioxygenation was faster than 1,2-c dioxygenation in the first 3-day culture. however, 1-hydroxy-2-naphthoic acid derived from 3,4-c dioxygenation degraded much slower than 2-hydroxy-1-naphthoic acid derived from 1,2-c dioxygenation. slow degradation of 1-hydroxy-2-naphthoic acid relative t ... | 2007 | 16491303 |
| remote interactions explain the unusual regioselectivity of lipase from pseudomonas cepacia toward the secondary hydroxyl of 2'-deoxynucleosides. | lipase from pseudomonas cepacia (pcl) surprisingly favors acylation of the secondary hydroxyl at the 3'-position over the primary hydroxyl at the 5'-position in 2'-deoxynucleosides by up to >98:1. catalytically productive tetrahedral intermediate analogues for both orientations were found by molecular modeling. however, acylation of the 3'-hydroxyl places the thymine base in the alternate hydrophobic pocket of pcl's substrate-binding site where it can hydrogen bond to the side-chain hydroxyls of ... | 2006 | 16491501 |
| cpg-modified plasmid dna encoding flagellin improves immunogenicity and provides protection against burkholderia pseudomallei infection in balb/c mice. | the plasmid dna encoding the flic gene of burkholderia pseudomallei combined with cpg oligodeoxynucleotide (odn) was injected intramuscularly into balb/c mice, resulting in the increased production of certain humoral antibodies and flagellin-specific spleen cell clonal expansion. cpg odn, as an immunoadjuvant, was added to the plasmid containing the flic gene in order to obtain ongoing expression in muscle for a long period. functional expression of flagellin from the constructed cpg-modified pl ... | 2006 | 16495541 |
| protection against heterologous burkholderia pseudomallei strains by dendritic cell immunization. | burkholderia pseudomallei, the causative agent of melioidosis, is a gram-negative bacterium which can cause either chronic infections or acute lethal sepsis in infected individuals. the disease is endemic in southeast asia and northern australia, but little is known about the mechanisms of protective immunity to the bacterium. in this study, we have developed a procedure to utilize dendritic cells in combination with cpg oligodeoxynucleotides as a vaccine delivery vector to induce protective imm ... | 2006 | 16495542 |
| a cpg oligonucleotide can protect mice from a low aerosol challenge dose of burkholderia mallei. | treatment with an oligodeoxynucleotide (odn) containing cpg motifs (cpg odn 7909) was found to protect balb/c mice from lung infection or death after aerosol challenge with burkholderia mallei. protection was associated with enhanced levels of gamma interferon (ifn-gamma)-inducible protein 10, interleukin-12 (il-12), ifn-gamma, and il-6. preexposure therapy with cpg odns may protect victims of a biological attack from glanders. | 2006 | 16495571 |
| monoclonal antibodies passively protect balb/c mice against burkholderia mallei aerosol challenge. | glanders is a debilitating disease with no vaccine available. murine monoclonal antibodies were produced against burkholderia mallei, the etiologic agent of glanders, and were shown to be effective in passively protecting mice against a lethal aerosol challenge. the antibodies appeared to target lipopolysaccharide. humoral antibodies may be important for immune protection against b. mallei infection. | 2006 | 16495574 |
| directed evolution to enhance secretion efficiency and thermostability of chitosanase from mitsuaria chitosanitabida 3001. | chitosanase (choa) from mitsuaria chitosanitabida 3001 was successfully evolved with secretion efficiency and thermal stability. the inactive choa mutant (g151d) gene was used to mutate by an error-prone pcr technique and mutant genes that restored chitosanase activity were isolated. two desirable mutants, designated m5s and m7t, were isolated. two amino acids, leu74 and val75, in the signal peptide of choa were changed to gln and ile respectively in the m7t mutant, in addition to the g151d muta ... | 2006 | 16495682 |
| binding properties of pyochelin and structurally related molecules to fpta of pseudomonas aeruginosa. | pyochelin (pch) is a siderophore that is produced in iron-limited conditions, by both pseudomonas aeruginosa and burkholderia cepacia. this iron uptake pathway could therefore be a target for the development of new antibiotics. pch is (4'r,2''r/s,4''r)-2'-(2-hydroxyphenyl)-3''-methyl-4',5',2'',3'',4'',5''-hexahydro-[4',2'']bithiazolyl-4''-carboxylic acid, and has three chiral centres located at positions c4', c2'' and c4''. in p.aeruginosa, this siderophore chelates iron in the extracellular med ... | 2006 | 16499928 |
| the slyb outer membrane lipoprotein of burkholderia multivorans contributes to membrane integrity. | slyb is a small lipoprotein of 158 amino acids which is conserved in different gram-negative bacteria. in contrast to other bacteria, where slyb is monocistronic, in burkholderia multivorans and in b. cenocepacia, slyb is the last gene of an operon comprising three open reading frames encoding a putative thiol peroxidase, a putative sugar kinase and slyb. b. multivorans slyb mutants produced elongated cells and filaments which were never observed in cultures of wild-type or slyb-complemented cel ... | 2006 | 16500084 |
| preparation of cross-linked enzyme aggregates by using bovine serum albumin as a proteic feeder. | addition of bovine serum albumin (bsa) as a proteic feeder facilitates obtaining cross-linked enzyme aggregates (cleas) in cases where the protein concentration in the enzyme preparation is low and/or the enzyme activity is vulnerable to the high concentration of glutaraldehyde required to obtain aggregates. cleas of pseudomonas cepacia lipase and penicillin acylase were prepared. clea of lipase prepared in the presence of bsa retained 100% activity whereas clea prepared without bsa retained onl ... | 2006 | 16500610 |
| molecular epidemiology and antibiotic susceptibility of burkholderia cepacia-complex isolates from an italian cystic fibrosis centre. | in order to further understanding of how different isolates of burkholderia cepacia complex persist, spread and cause disease, b. cepacia-complex isolates from 60 patients attending the cystic fibrosis centre of verona, italy, between 1997 and 2002 were analyzed. strains were examined for species, presence of putative epidemic and virulence markers (i.e., cbla and the b. cepacia epidemic-strain marker [bcesm]), genetic relatedness and antibiotic susceptibility. forty-five percent of patients wer ... | 2006 | 16501927 |
| purification and characterization of fenitrothion hydrolase from burkholderia sp. nf100. | the organophosphorus pesticide hydrolase was purified to homogeneity from burkholderia sp. nf100 by detergent extraction of the cell membrane fraction, anion-exchange, chromatofocusing, and gel filtration chromatographies. the purified enzyme had a molecular mass of 55 kda and a pi 5.8, and the hydrolase activity was strongly inhibited by edta, dithiothreitol (dtt), hg2+ and 1,10-phenanthroline. the optimum ph and temperature for the enzyme activity were 8.0 and 40 degrees c, respectively. the e ... | 2006 | 16503297 |
| multiple visceral abscess in a case of melioidosis. | | 2006 | 16505577 |
| microbial contamination of nebulization solution and its measures. | we evaluated the microbial contamination of nebulization solutions in medication cups from a total of 76 ultrasonic nebulizers in use in 10 hospitals. in addition, an interview survey was given to nurses to evaluate the disinfection methods of these ultrasonic nebulizers. of a total of 76 nebulization solution samples, 11 (14.5%) were contaminated with 10-10(2) colony-forming units (cfu)/ml and 9 (11.8%) with 10(3)-10(5) cfu/ml. the major contaminants were glucose non-fermentative bacilli such a ... | 2006 | 16508154 |
| cometabolic degradation of tce vapors in a foamed emulsion bioreactor. | effective cometabolic biodegradation of trichloroethylene (tce) vapors in a novel gas-phase bioreactor called the foamed emulsion bioreactor (febr) was demonstrated. toluene vapors were used as the primary growth substrate for burkholderia cepacia g4 which cometabolically biodegraded tce. batch operation of the reactor with respect to the liquid feed showed a drastic decrease of tce and toluene removal over time, consistent with a loss of metabolic activity caused by the exposure to tce metaboli ... | 2006 | 16509352 |
| purification, crystallization and preliminary crystallographic analysis of dehiva, a dehalogenase from burkholderia cepacia mba4. | dehiva is one of two dehalogenases produced by the soil- and water-borne bacterium burkholderia cepacia mba4. it acts to break down short-chain halogenated aliphatic acids through a nucleophilic attack and subsequent hydrolysis of an enzyme-substrate intermediate to remove the halide ions from l-enantiomers substituted at the c2 position (e.g l-2-monochloropropionic acid). dehalogenases are an important group of enzymes that are responsible for breaking down a diverse range of halogenated enviro ... | 2005 | 16511015 |
| crystallization and crystal manipulation of a steric chaperone in complex with its lipase substrate. | bacterial lipases that are secreted via the type ii secretion pathway require a lipase-specific foldase in order to obtain their native and biologically active conformation in the periplasmic space. the lipase-foldase complex from burkholderia glumae (319 and 333 residues, respectively) was crystallized in two crystal forms. one crystal form belongs to space group p3(1)21 (p3(2)21), with unit-cell parameters a = b = 122.3, c = 98.2 a. a procedure is presented which improved the diffraction of th ... | 2005 | 16511160 |
| computed tomography characteristics of burkholderia pseudomallei liver abscess. | we report 3 cases of burkholderia pseudomallei liver abscesses that have a characteristic "honeycomb" appearance on computed tomography scans. such a finding should prompt physicians to include b. pseudomallei infection in the differential diagnoses of liver abscesses and consider initiation of empirical therapy for melioidosis in high-risk patients from areas of endemicity. | 2006 | 16511765 |
| melioidosis in imported non-human primates. | in 1969, five cases of melioidosis in three separate outbreaks were diagnosed in nonhuman primates in the united states. in the first outbreak, two stump-tailed macaque monkeys (macaca arctoides) developed signs of the disease approximately 6 months after purchase. a third animal, a chimpanzee (pan troglodytes), probably acquired its infection from one of these monkeys. two other unrelated cases involving a pig-tailed monkey (macaca nemestrina) and a rhesus monkey (macaca mulatta) were diagnosed ... | 1970 | 16512115 |
| [molecular typing of the burkholderia pseudomallei strains with different resistance to antibiotics]. | wild type strains of burkholderia pseudomallei, spontaneous mutants with high resistance to fluoroquinolones and ceftazidime, and tn5-induced mutants with reduced resistance level were studied using polymorphic and gene-specific dna fingerprinting. cluster analysis of genomic dna patterns obtained using pcr with arbitrary primer (5'-gtttcgctcc-3') and primer specific to the class i integrase intll gene (5'-cctcccgcacgatgatc-3') was performed. according to the dna pattern conformity, the distinct ... | 2006 | 16512603 |
| a complete lipopolysaccharide inner core oligosaccharide is required for resistance of burkholderia cenocepacia to antimicrobial peptides and bacterial survival in vivo. | burkholderia cenocepacia is an important opportunistic pathogen of patients with cystic fibrosis. this bacterium is inherently resistant to a wide range of antimicrobial agents, including high concentrations of antimicrobial peptides. we hypothesized that the lipopolysaccharide (lps) of b. cenocepacia is important for both virulence and resistance to antimicrobial peptides. we identified hlda and hldd genes in b. cenocepacia strain k56-2. these two genes encode enzymes involved in the modificati ... | 2006 | 16513737 |
| melioidosis: the johor bahru experience. | a 5 year retrospective review of cases of melioidosis was carried out in sultanah aminah hospital, johor bahru. there were 44 new cases of melioidosis which was proven by either blood or pus culture growing burkholderia pseudomallei from the period between january 1999 and december 2003. of these, 38 (86.4%) were males compared to only 6 (13.6%) females. thirty-one (70.5%) were malays, 7 (15.9%) were chinese, 5 (11.4%) were indians and 1 (2.2%) was a sarawakian. the peak age group was between 50 ... | 2005 | 16515111 |
| the structure of the o-specific polysaccharide of the lipopolysaccharide from burkholderia gladioli pv. agaricicola. | a neutral o-specific polysaccharide containing d-mannose, d-rhamnose and d-galactose was obtained by mild acid hydrolysis of the lipopolysaccharide of the plant pathogenic bacterium burkholderia gladioli pv. agaricicola. by means of compositional analyses and nmr spectroscopy, the chemical repeating unit of the polymer was identified as a linear trisaccharide of the structure shown below, in which the mannose residue was quantitatively acetylated at c-2. [carbohydrate structure: see text] | 2006 | 16516174 |
| rhizobitoxine modulates plant-microbe interactions by ethylene inhibition. | bradyrhizobium elkanii produces rhizobitoxine, an enol-ether amino acid, which has been regarded as a phytotoxin because it causes chlorosis in soybeans. however, recent studies have revealed that rhizobitoxine plays a positive role in establishing symbiosis between b. elkanii and host legumes: rhizobitoxine enhances the nodulation process by inhibiting acc (1-aminocyclopropane-1-carboxylate) synthase in the ethylene biosynthesis of host roots. b. elkanii rtxa and rtxc genes are required for rhi ... | 2006 | 16516430 |
| generation of novel-substrate-accepting biphenyl dioxygenases through segmental random mutagenesis and identification of residues involved in enzyme specificity. | aryl-hydroxylating dioxygenases are of interest for the degradation of persistant aromatic pollutants, such as polychlorobiphenyls (pcbs), or as catalysts for the functionalization of aromatic scaffolds. in order to achieve dioxygenation of technical mixtures of pcbs, enzymes with broadened or altered substrate ranges are essential. to alter the substrate specificity of the biphenyl dioxygenase (bpha) of burkholderia xenovorans lb400, we applied a directed evolution approach that used structure- ... | 2006 | 16517671 |
| use of various common isolation media to evaluate the new vitek 2 colorimetric gn card for identification of burkholderia pseudomallei. | the use of automated systems in the modern microbiology laboratory is becoming commonplace as the pressure of cost containment impacts on staff resources. with increased international travel and threats of bioterrorism, recognition and accurate identification of organisms such as burkholderia pseudomallei is important. in areas where this organism is endemic, identification is not usually problematic. this study evaluates the performance of the new vitek 2 colorimetric gn card for the identifica ... | 2006 | 16517866 |
| evaluation of the phoenix 100 id/ast system and nid panel for identification of enterobacteriaceae, vibrionaceae, and commonly isolated nonenteric gram-negative bacilli. | the phoenix 100 id/ast system (becton dickinson co., sparks, md.) is an automated system for the identification and antimicrobial susceptibility testing of bacterial isolates. this system with its negative identification (nid) panel was evaluated for its accuracy in the identification of 507 isolates of the family enterobacteriaceae, 57 other nonenteric gram-negative isolates that are commonly isolated in clinical microbiology laboratories, and 138 isolates of the family vibrionaceae. all of the ... | 2006 | 16517878 |
| differentiation of species combined into the burkholderia cepacia complex and related taxa on the basis of their fatty acid patterns. | using the established commercial system sherlock (midi, inc.), cellular fatty acid methyl ester analysis for differentiation among burkholderia cepacia complex species was proven. the identification key based on the diagnostic fatty acids is able to discern phenotypically related ralstonia pickettii and pandoraea spp. and further distinguish burkholderia pyrrocinia, burkholderia ambifaria, and burkholderia vietnamiensis. | 2006 | 16517920 |
| the effect of methanolic extract of tamarindus indica linn. on the growth of clinical isolates of burkholderia pseudomallei. | burkholderia pseudomallei (pseudomonas pseudomallei) causes melioidosis, a life-threatening infection common among paddy cultivators in southeast asian countries. no plant materials have been investigated for its activity against b. pseudomallei. therefore, a preliminary study was carried out using disc diffusion and minimum inhibitory concentration (mic) methods to evaluate the anti-b. pseudomallei activity of five indian medicinal plants documented to have been used for several ailments in the ... | 2005 | 16518004 |
| structure of a membrane-based steric chaperone in complex with its lipase substrate. | secretion via the type ii secretion pathway in gram-negative bacteria often relies crucially on steric chaperones in the periplasm. here, we report the crystal structure of the soluble form of a lipase-specific foldase (lif) from burkholderia glumae in complex with its cognate lipase. the structure reveals how lif uses a novel alpha-helical scaffold to embrace lipase, thereby creating an unusually extensive folding platform. | 2006 | 16518399 |
| global assessment of the antimicrobial activity of polymyxin b against 54 731 clinical isolates of gram-negative bacilli: report from the sentry antimicrobial surveillance programme (2001-2004). | in total, 54 731 gram-negative bacilli isolated worldwide between 2001 and 2004 from diverse sites of infection were tested for susceptibility to polymyxin b by the broth reference microdilution method, with interpretation of results according to clsi (formerly nccls) guidelines. polymyxin b showed excellent potency and spectrum against 8705 pseudomonas aeruginosa and 2621 acinetobacter spp. isolates (mic50, < or = 1 mg/l and mic90, 2 mg/l for both pathogens). polymyxin b resistance rates were s ... | 2006 | 16524407 |
| burkholderia pseudomallei rpos regulates multinucleated giant cell formation and inducible nitric oxide synthase expression in mouse macrophage cell line (raw 264.7). | burkholderia pseudomallei is the causative agent of melioidosis. this bacterium can invade and survive inside the phagocytic and nonphagocytic cells. after internalization, the bacteria can escape from the membrane-bound phagosome into the cytoplasm. internalised b. pseudomallei can also induce a cell-to-cell fusion, resulting in a multinucleated giant cell (mngc) formation. in the present study, we demonstrated that b. pseudomallei rpos null mutant was similar to its wild type parent in its abi ... | 2006 | 16524693 |
| pulsed-field gel electrophoresis as a discriminatory typing technique for the biothreat agent burkholderia mallei. | pulsed-field gel electrophoresis (pfge) and multilocus sequence typing (mlst) was used to type 21 laboratory strains of burkholderia mallei. we demonstrated good resolution by pfge together with clustering of some geographically related isolates, and confirmed previous observations that b. mallei is clonal as defined by mlst. | 2006 | 16525089 |
| lipopolysaccharide heterogeneity among burkholderia pseudomallei from different geographic and clinical origins. | heterogeneous patterns were obtained for lipopolysaccharide (lps) from 1,327 burkholderia pseudomallei isolates by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, silver staining, and immunoblot analysis. two lps serotypes (a and b) possessing different ladder profiles and a rough lps without ladder appearances were identified. all three lps types were antigenically distinct by immunoblotting. the predominant type a (97%) produced the lowest amount of biofilm. the two less common type ... | 2006 | 16525090 |
| substrate specificity of lipases in alkoxycarbonylation reaction: qsar model development and experimental validation. | although lipases are known to catalyze alkoxycarbonylation reactions in organic solvents, the existing knowledge base on their substrate specificity in alkoxycarbonylation reaction is sparse. moreover, models to predict substrate specificity have not been reported. here, we report the experimentally measured rate constants for 180 acyl donor-alcohol pairs and demonstrate the two-step synthesis of over 70 disubstituted carbonate products from simple precursors such as diphenyl carbonate and alcoh ... | 2006 | 16528758 |
| tracking the response of burkholderia cepacia g4 5223-pr1 in aquifer microcosms. | the introduction of bacteria into the environment for bioremediation purposes (bioaugmentation) requires analysis and monitoring of microbial population dynamics to define persistence and activity from both efficacy and risk assessment perspectives. burkholderia cepacia g4 5223-pr1 is a tn5 insertion mutant which constitutively expresses a toluene ortho-monooxygenase that degrades trichloroethylene (tce). this ability of g4 5223-pr1 to degrade tce without aromatic induction may be useful for bio ... | 1995 | 16534928 |
| nickel-resistant bacteria from anthropogenically nickel-polluted and naturally nickel-percolated ecosystems. | dna fragments harboring the nickel resistance determinants from bacteria isolated from anthropogenically polluted ecosystems in europe and zaire were compared with those harboring the nickel resistance determinants from bacteria isolated from naturally nickel-percolated soils from new caledonia by dna-dna hybridization. the biotinylated dna probes were derived from the previously described alcaligenes eutrophus ch34, alcaligenes xylosoxidans 31a, alcaligenes denitrificans 4a-2, and klebsiella ox ... | 1995 | 16535048 |
| burkholderia pseudomallei requires zn(sup2+) for optimal exoprotease production in chemically defined media. | the effects of various medium components on exoprotease production by burkholderia pseudomallei were studied in order to understand the production of virulence factors by this pathogenic organism. feso(inf4), nacl, and mgso(inf4) do not significantly affect exoprotease activity, and cacl(inf2) has only a slight influence. conversely, zncl(inf2) plays a fundamental role since it drastically increases exoprotease production. | 1995 | 16535112 |
| bacteriocin typing of burkholderia (pseudomonas) solanacearum race 1 of the french west indies and correlation with genomic variation of the pathogen. | burkholderia solanacearum race 1 isolates indigenous to the french west indies were characterized by bacteriocin typing and two genomic fingerprinting methods: pulsed-field gel electrophoresis of genomic dna digested by rare-cutting restriction endonucleases (rc-pfge) and pcr with primers corresponding to repetitive extragenic palindromic (rep), enterobacterial repetitive intergenic consensus (eric), and box elements (collectively known as rep-pcr). the survey comprised 24 reference strains and ... | 1996 | 16535234 |
| degradation of toluene and trichloroethylene by burkholderia cepacia g4 in growth-limited fed-batch culture. | burkholderia (pseudomonas) cepacia g4 was cultivated in a fed-batch bioreactor on either toluene or toluene plus trichloroethylene (tce). the culture was allowed to reach a constant cell density under conditions in which the amount of toluene supplied equals the maintenance energy demand of the culture. compared with toluene only, the presence of tce at a toluene/tce ratio of 2.3 caused a fourfold increase in the specific maintenance requirement for toluene from 22 to 94 nmol mg of cells (dry we ... | 1996 | 16535277 |
| temporal variation in genetic diversity and structure of a lotic population of burkholderia (pseudomonas) cepacia. | the genetic structure and temporal patterns of genetic diversity in a population of burkholderia (pseudomonas) cepacia, isolated from a southeastern blackwater stream, were investigated by multilocus enzyme electrophoresis. allelic variation in seven structural gene loci was monitored at a single stream location at 0, 6, 12, and 24 h and at 2, 4, 8, 16, and 32 days. over the length of the study, 217 isolates were collected, from which 65 unique electrophoretic types (ets) were identified. most o ... | 1996 | 16535308 |
| evidence for acquisition in nature of a chromosomal 2,4-dichlorophenoxyacetic acid/(alpha)-ketoglutarate dioxygenase gene by different burkholderia spp. | we characterized the gene required to initiate the degradation of 2,4-dichlorophenoxyacetic acid (2,4-d) by the soil bacterium burkholderia sp. strain tfd6, which hybridized to the tfda gene of the canonical 2,4-d catabolic plasmid pjp4 under low-stringency conditions. cleavage of the ether bond of 2,4-d by cell extracts of tfd6 proceeded by an (alpha)-ketoglutarate-dependent reaction, characteristic of tfda (f. fukumori and r. p. hausinger, j. bacteriol. 175:2083-2086, 1993). the tfd6 tfda gene ... | 1996 | 16535356 |
| bacteria are omnipresent on phanerochaete chrysosporium burdsall. | bacteria have been isolated from 10 different strains of phanerochaete chrysosporium, a white rot fungus which degrades lignocellulosic materials. the investigations showed that one or more bacterial species were always associated with the fungus. various attempts to eliminate the bacteria on the fungus were unsuccessful. three different bacterial species were isolated and identified. one of these was agrobacterium radiobacter, while another may represent a new taxon close to the genus burkholde ... | 1996 | 16535357 |
| genetic diversity through the looking glass: effect of enrichment bias. | the effect of enrichment bias on the diversity of 2,4-dichlorophenoxyacetate (2,4-d)-degrading (2,4-d(sup+)) bacteria recovered from soil was evaluated by comparing the diversity of isolates obtained by direct plating to the diversity of isolates obtained from 85 liquid batch cultures. by the two methods, a total of 159 isolates were purified from 1 g of soil and divided into populations based on repeated extragenic palindromic sequence pcr (rep-pcr) genomic fingerprints. approximately 42% of th ... | 1997 | 16535569 |
| microbial succession during a field evaluation of phenol and toluene as the primary substrates for trichloroethene cometabolism. | microbial community composition and succession were studied in an aquifer that was amended with phenol, toluene, and chlorinated aliphatic hydrocarbons to evaluate the effectiveness of these aromatic substrates for stimulating trichloroethene (tce) bioremediation. samples were taken after the previous year's field studies, which used phenol as the primary substrate, and after three successive monthly treatments of phenol plus 1,1-dichloroethene (1,1-dce) plus tce, phenol plus tce, and toluene pl ... | 1997 | 16535576 |
| phenol- and toluene-degrading microbial populations from an aquifer in which successful trichloroethene cometabolism occurred. | we characterized the bacterial populations that grew in a moffett field, calif., aquifer following three sequential field tests of phenol- or toluene-driven cometabolism of trichloroethene (tce). reducing the toluene and phenol concentrations in most-probable-number (mpn) tubes from 50 to 5 ppm increased the population density measured for these degraders by 1.5 and 1 log units, respectively, suggesting that natural populations might be quite sensitive to these substrates. phenol and toluene deg ... | 1997 | 16535577 |
| toluene 2-monooxygenase-dependent growth of burkholderia cepacia g4/pr1 on diethyl ether. | aerobic bacterial growth on aromatic hydrocarbons typically requires oxygenase enzymes, which are known to fortuitously oxidize nongrowth substrates. in this study, we found that oxidation of diethyl ether by toluene 2-monooxygenase supported more rapid growth of burkholderia cepacia g4/pr1 than did the aromatic substrates n-propylbenzene and o-xylene. the wild-type burkholderia cepacia g4 failed to grow on diethyl ether. purified toluene 2-monooxygenase protein components oxidized diethyl ether ... | 1997 | 16535583 |
| metabolism of chlorotoluenes by burkholderia sp. strain ps12 and toluene dioxygenase of pseudomonas putida f1: evidence for monooxygenation by toluene and chlorobenzene dioxygenases. | the degradation of toluene by pseudomonas putida f1 and of chlorobenzenes by burkholderia sp. strain ps12 is initiated by incorporation of dioxygen into the aromatic nucleus to form cis-dihydrodihydroxybenzenes. toluene-grown cells of p. putida f1 and 3-chlorobenzoate-grown cells of burkholderia sp. strain ps12 were found to monooxygenate the side chain of 2- and 3-chlorotoluene to the corresponding chlorobenzyl alcohols. further metabolism of these products was slow, and the corresponding chlor ... | 1997 | 16535607 |
| mineralization of 4-chlorodibenzofuran by a consortium consisting of sphingomonas sp. strain rw1 and burkholderia sp. strain jws. | the dibenzofuran-degrading bacterium sphingomonas sp. strain rw1 (r.-m. wittich, h. wilkes, v. sinnwell, w. francke, and p. fortnagel, appl. environ. microbiol. 58:1005-1010, 1992) attacks 4-chlorodibenzofuran on the unsubstituted aromatic ring via distal dioxygenation adjacent to the ether bridge to produce 3(prm1)-chloro-2,2(prm1),3-trihydroxybiphenyl, which was identified by nuclear magnetic resonance spectroscopy and mass spectrometry. the compound is subsequently meta cleaved, and the respe ... | 1997 | 16535686 |
| photo quiz 2. right lower lobe, lobar pneumonia: pseudomonas cepacia. | | 2005 | 16538911 |
| melioidosis: insights into the pathogenicity of burkholderia pseudomallei. | burkholderia pseudomallei is a potential bioterror agent and the causative agent of melioidosis, a severe disease that is endemic in areas of southeast asia and northern australia. infection is often associated with bacterial dissemination to distant sites, and there are many possible disease manifestations, with melioidosis septic shock being the most severe. eradication of the organism following infection is difficult, with a slow fever-clearance time, the need for prolonged antibiotic therapy ... | 2006 | 16541135 |
| taxonomy and pathogenesis of the burkholderia cepacia complex. | patients with cystic fibrosis (cf) are susceptible to chronic respiratory infection with a number of bacterial pathogens. the burkholderia cepacia complex bacteria are problematic cf pathogens because (i) they are very resistant to antibiotics, making respiratory infection difficult to treat and eradicate; (ii) infection with these bacteria is associated with high mortality in cf; (iii) they may spread from one cf patient to another, leading to considerable problems for both patients and carers; ... | 2005 | 16541604 |
| endotoxin-like properties of a rhamnolipid exotoxin from burkholderia (pseudomonas) plantarii: immune cell stimulation and biophysical characterization. | here we report on the purification, structural characterization, and biological activity of a glycolipid, 2-o-alpha-l-rhamnopyranosyl-alpha-l-rhamnopyranosyl-alpha(r)-3-hydroxytetradecanoyl-(r)-3-hydroxytetradecanoate (rl-2,2(14)) produced by burkholderia (pseudomonas) plantarii. rl-2,2(14) is structurally very similar to a rhamnolipid exotoxin from pseudomonas aeruginosa and identical to the rhamnolipid of burkholderia pseudomallei, the causative agent of melioidosis. interestingly, rl-2,2(14) ... | 2006 | 16542152 |
| nitrate-dependent anaerobic carbon monoxide oxidation by aerobic co-oxidizing bacteria. | two dissimilatory nitrate-reducing (burkholderia xenovorans lb400 and xanthobacter sp. str. cox) and two denitrifying isolates (stappia aggregata iam 12614 and bradyrhizobium sp. str. cpp), previously characterized as aerobic co oxidizers, consumed co at ecologically relevant levels (<100 ppm) under anaerobic conditions in the presence, but not absence, of nitrate. none of the isolates were able to grow anaerobically with co as a carbon or energy source, however, and nitrate-dependent anaerobic ... | 2006 | 16542399 |
| importance of mycorrhization helper bacteria cell density and metabolite localization for the pinus sylvestris-lactarius rufus symbiosis. | mycorrhization helper bacteria, paenibacillus sp. ejp73 and burkholderia sp. ejp67, were used to study the importance of bacterial inoculum dose and bacterial derived soluble and volatile metabolites localization for enhancing mycorrhiza formation in the pinus sylvestris-lactarius rufus symbiosis, using a laboratory based microcosm. ejp73 and ejp67 produced different responses in relation to the inoculum dose; ejp73 significantly enhanced mycorrhiza formation to the same degree at all doses test ... | 2006 | 16542402 |
| endobacteria in some ectomycorrhiza of scots pine (pinus sylvestris). | the diversity of cultivable endobacteria associated with four different ectomycorrhizal morphotypes (suillus flavidus, suillus variegatus, russula paludosa and russula sp.) of scots pine (pinus sylvestris) was analysed by restriction fragment length polymorphism profiling of pcr-amplified rdna intergenic spacer regions and by sequence analyses of 16s rrna genes. ectomycorrhizal root tip surface-sterilization methods were developed and assessed for their efficiencies. bacterial communities from s ... | 2006 | 16542403 |
| six cases of suppurative lymphadenitis caused by burkholderia pseudomallei infection. | melioidosis, an infection caused by burkholderia pseudomallei, is endemic in southeast asia and northern australia. it can have various presentations, ranging from acute septicaemia to chronic localized infection. uncomplicated, localized infection usually involves skin or subcutaneous tissues. suppurative lymphadenitis caused by melioidosis has been rarely encountered by clinicians practicing in endemic areas. we encountered six cases of suppurative lymphadenitis caused by burkholderia pseudoma ... | 2006 | 16542691 |
| cervical necrotizing fasciitis: a novel clinical presentation of burkholderia cepacia infection. | necrotizing fasciitis is a soft-tissue infection characterized by progressive destruction of fascia and adipose tissue which may not involve the skin. cervical necrotizing fascitis (cnf) is an uncommon clinical entity. the development of cnf is frequently related to synergistic infections of aerobic and anaerobic organisms of the upper aerodigestive tract. we describe the first case of cnf due to multi-drug resistant burkholderia cepacia and peptostreptococcus infection in an immuno-competent pa ... | 2006 | 16545459 |
| development of a polymerase chain reaction assay for the specific identification of burkholderia mallei and differentiation from burkholderia pseudomallei and other closely related burkholderiaceae. | burkholderia mallei and burkholderia pseudomallei, the etiologic agents responsible for glanders and melioidosis, respectively, are genetically and phenotypically similar and are category b biothreat agents. we used an in silico approach to compare the b. mallei atcc 23344 and b. pseudomallei k96243 genomes to identify nucleotide sequences unique to b. mallei. five distinct b. mallei dna sequences and/or genes were identified and evaluated for polymerase chain reaction (pcr) assay development. g ... | 2006 | 16546342 |
| clinical guideline for diagnosis and management of melioidosis. | melioidosis is an emerging infection in brazil and neighbouring south american countries. the wide range of clinical presentations include severe community-acquired pneumonia, septicaemia, central nervous system infection and less severe soft tissue infection. diagnosis depends heavily on the clinical microbiology laboratory for culture. burkholderia pseudomallei, the bacterial cause of melioidosis, is easily cultured from blood, sputum and other clinical samples. however, b. pseudomallei can be ... | 2006 | 16547571 |
| antimicrobial activity of a novel peptide deformylase inhibitor, lbm415, tested against respiratory tract and cutaneous infection pathogens: a global surveillance report (2003-2004). | to evaluate the spectrum of activity and potency of lbm415, the first of the peptide deformylase inhibitor (pdfi) class to be developed for treatment of community-acquired respiratory tract infections and uncomplicated skin and soft tissue infections (ussti), against a large, contemporary international collection of targeted pathogens collected during 2003-2004. | 2006 | 16549511 |
| stent-graft for recurrent melioidosis mycotic aortic aneurysm. | melioidosis is a tropical disease caused by burkholderia pseudomallei and is prevalent in south east asia and northern australia. it can infect any organ system and is potentially deadly. melioidosis causing a mycotic aneurysm of the aorta is rare. we present a patient with a melioidosis mycotic aneurysm of the descending aorta presenting with fever and right pleural effusion, managed successfully with initial dacron graft repair with staged omental reinforcement, and subsequent endovascular ste ... | 2006 | 16551809 |
| carbazole biodegradation in gas oil/water biphasic media by a new isolated bacterium burkholderia sp. strain imp5gc. | to select carbazole-degrading bacteria able to survive and metabolize carbazole in biphasic organic-water media and to study the factors affecting carbazole degradation in such conditions. | 2006 | 16553728 |
| characterization of psychrotolerant heterotrophic bacteria from finnish lapland. | a total of 331 aerobic heterotrophic bacterial strains were isolated from various ecosystems of finnish lapland (68-69 degrees n) including forest soil, arctic alpine-tundra soil, stream water, lake and mire sediments, lichen and snow algae. whole cell fatty acid and 16s rrna gene sequence analysis and microscopy indicated that the isolates were dominated by gram-negative bacteria, while only 20 gram-positive strains were isolated. based on 16s rrna gene sequences the isolates were members of al ... | 2006 | 16564959 |
| in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis. | investigation of the in vitro activity of tigecycline against burkholderia pseudomallei and burkholderia thailandensis revealed that the inhibition zone diameters of tigecycline against all isolates were >or=20 mm and that the mic50 values were 0.5 and 1 microg/ml and the mic90 values were 2 and 1.5 microg/ml for b. pseudomallei and b. thailandensis, respectively. | 2006 | 16569883 |