| school milk and meals. | | 1978 | 76899 |
| synthetic concanavalin a receptors and erythrocyte agglutination. | | 1978 | 76989 |
| a cytofluorometric method of counting trypanosomes. | a rapid automated method of counting trypanosomes was developed using cytofluorometric methods. organisms were vitally stained with acridine orange, and deae cellulose separated t. congolese were accurately counted at all concentrations. t. congolense in heparinized whole bovine blood were also counted accurately at levels greater than 1,000 per microliter. at lower levels, small fluorescent particles in whole blood interfered with the counts. | 1978 | 77072 |
| [cross reactivity of mammalian c-type viruses by membrane antigens in the virion precipitation test]. | | 1978 | 77083 |
| ultrastructure of babesia major vermicules from the tick haemaphysalis punctata as demonstrated by negative staining. | the ultrastructure of babesia major vermicules was studied in samples derived from the haemolymph of haemaphysalis punctata adults and negatively stained with phosphotungstic acid. most of the organelles observed were typical of those found in apicomplexan parasites. these were the apical complex with the polar ring and the ribs, micronemes and subpellicular microtubules. the number of ribs was 27 or 28. the outer membrane of the pellicle was composed of a large number of fibrils running along t ... | 1978 | 77093 |
| [karyometric studies of cattle hypothalamus following application of sex steroids]. | | 1977 | 77094 |
| a receptor-immunoassay for the determination of the specificity of anti-hcg-beta sera. | in the receptor-immunoassay, the binding of anti-hcg-beta sera to 125i-hcg and 125i-hlh is measured in the presence of bovine corpus luteum "receptor". anti-sera specific for hcg show little binding with 125i-hlh. the receptor-immunoassay permits an evaluation of specificity, affinity and the ability of anti-hcg-beta sera to compete with the "receptor" for binding to 125i-hcg. | 1978 | 77119 |
| fluorescence studies of the interactions of serum albumin and rat alpha1-fetoprotein with aflatoxin b1. specificity and binding parameters. | the interaction of bovine serum albumin and rat alpha1-fetoprotein with aflatoxin b1 has been followed by the fluorescence quenching of the protein in presence of the ligand. the binding parameters (n, number of sites and kd, dissociation constant) have been determined for the bovine serum albumin-alflatoxin b1 system: n = 3.5 and kd = 3.1 +/- 0.5 . 10(-5) m and for the alpha-fetoprotein-aflatoxin system: n = 4 and kd = 3.7 +/-0.5 . 10(-5) m. the competition of anilino-naphthalene-sulfonate and ... | 1978 | 77164 |
| induced robertsonian fusions and tandem translocations in mammalian cell cultures. | cultures of a cattle cell line and a peromyscus eremicus cell line recovering from a pulse-treatment with mitomycin c, actinomycin d, 33258 hoechst, and nitrosoguanidine exhibited translocations between chromosomes at the centromeric regions (robertsonian fusions) as well as between centromere and telomere and between telomeres (tandem translocations). the frequency of robertsonian fusions was found to be dose-dependent and duration-dependent with the mitomycin treatment. biarmed chromosomes res ... | 1978 | 77213 |
| beta-lapachone, an inhibitor of oncornavirus reverse transcriptase and eukaryotic dna polymerase-alpha. inhibitory effect, thiol dependence and specificity. | beta-lapachone is a naturally occuring compound that can be isolated from a number of tropical trees. it is shown to be a potent inhibitor of reverse transcriptase activity from both avian myeloblastosis virus and rauscher murine leukaemia virus. in addition, it affects eukaryotic dna-dependent dna polymerase-alpha activity: 50% inhibition is reached in 60-min incubation time by about 8 micron beta-lapachone. enzyme activity is inhibited irrespective of the purity of the enzyme used or of the am ... | 1978 | 77223 |
| cycle synchronization and induction in zebu cattle with chlormadinone acetate in differing dosage forms: effects of season. | | 1978 | 77234 |
| immunochemical properties of alpha-fetoprotein (afp) and antibodies to autologous afp. | alpha-fetoprotein (afp) is a strong immunogen when injected into another species, but does not elicit an immune response in the species of origin of the afp. afps from different mammalian species are crossreactive. afp and albumin show sequence homology. they do not crossreact in their native state, but their unfolded (reduced and carboxamidomethylated) forms are immunologically crossreactive. antibodies to autologous afp can be induced in animals by immunization with heterologous or modified ho ... | 1978 | 77251 |
| measurement of antibody and antigen concentrations with a sodium sulphate glutaraldehyde technique. | gamma-globulins can be crosslinked at low concentrations by simultaneous treatment with sodium sulphate and glutaraldehyde (ssg-method). this precipitate can be used for the assay of antibody or antigens. examples are given for the measurement of (1) anti-dinitrophenyl antibody by reaction of the precipitate with 125i-dinitrophenylovalbumin, (2) iga by inhibition of an anti-iga precipitate with an 125i-labelled iga myeloma protein, (3) idiotypic antibody by inhibition of the binding of a 125i-la ... | 1978 | 77296 |
| an immunosuppressive factor in serum of rabbits lethally infected with the herpesvirus of bovine malignant catarrhal fever. | virus-neutralizing antibody does not protect animals challenged with the herpesvirus of bovine malignant catarrh; therefore, other parameters of the immune response were investigated. in vitro transformation of peripheral blood lymphocytes with mitogens and specific antigens was monitored throughout the course of disease in lethally infected new zealand white rabbits. the whole-blood culture method was used. transformation with mitogens was normal throughout the incubation period but fell precip ... | 1978 | 77299 |
| oral cromoglycate in treatment of adverse reactions to foods. | 20 subjects with adverse reactions to foods were given oral cromoglycate and placebo separately for seven days each. while on each medication they were given, for four consecutive days, a food to which they were sensitive. adverse reactions were prevented in 14 subjects. these results suggest that oral cromoglycate is of value in the treatment of some patients with adverse reactions to foods. | 1978 | 77367 |
| experimental study on cerebral vasospasm: contractile proteins in smooth muscle of bovine carotid artery. | | 1978 | 77486 |
| the immune responses to antigenic variants of babesia bigemina in the bovine. | four babesia bigemina stabilates were used to determine the immune response of cattle to acute and chronic blood- and tick-borne infections. thirty-two intact calves were divided into 16 groups of two and each calf was inoculated with infective b bigemina erythrocytic stabilates. twenty-eight days later they were challanged with homologous and heterologous stabilates, and monitored for an additional 20 days. the hosts' apparently reduced response to homologous challenge but marked immune respon ... | 1978 | 77539 |
| application of bradford's protein assay to adrenal gland subcellular fractions. | | 1978 | 77646 |
| failure of fenoprofen to affect the corneal immunologic reaction. | in rabbits whose corneas had been subjected with bovine gamma globulin, fenoprofen administered subconjunctivally failed to alter the animals' inflammatory or immunologic responses. | 1978 | 77651 |
| effects of hexachlorocyclohexane isomers on the mitogenic response of bovine lymphocytes. | | 1978 | 77667 |
| suppressive factors in amniotic fluid and newborn serum: is alpha-fetoprotein involved? | | 1978 | 77739 |
| haptenic antibody induced by n-phthaloylated ox insulin in the hartley guinea-pig. | female hartley guinea-pigs were immunized with n-phthaloylated ox insulin (96% na1,nb1,nb29-triphthaloyl ox insulin) using h. pertussis as adjuvant. haptenic antibody, which was found in antisera taken 20 days after secondary immunization, was assessed for cross-reaction with a series of n-acylated ox insulins. cross-reaction readily occurred between haptenic antibody and the partial hapten-bearing antigens, na1-monophthaloyl and nb1-monophthaloyl ox insulins. the immunochemical data suggested t ... | 1978 | 77750 |
| [studies on d-penicillamine (1): inhibitory effect of d-penicillamine on the heat-cu++ induced denaturation of human gamma-globulin (author's transl)]. | the inhibitory effect of d-penicillamine on the denaturation of human gamma-globulin induced by heat and cu++ was compared with the action of other agents such as antirheumatic drugs, anti-inflammatory drugs, sh reagents, sh inhibitors and chelating reagents. the denaturation of human gamma globulin was induced by cu++ at 10 micrometer ("cu++ induced denaturation") and was further increased by heating at 63 degrees c for 3 hr in the presence of cu++ ("total denaturation"). thus the value obtaine ... | 1978 | 77827 |
| specificity of murine delayed-type hypersensitivity to conjugates of large or small haptens on protein carriers bearing lipid groups. | delayed-type hypersensitivity (dh) in the mouse was provoked with different hapten-carrier complexes mixed with the cationic, surface-active lipid, dimethyl dioctadecyl ammonium bromide (dda). dh was measured as footpad swelling. conjugates of bovine serum albumin (bsa) with the small haptens dinitrophenyl (dnp), 'arsonate' (ars) and 'sulphonate' (sulph) served to generate strong dh reactions towards the homologous antigen. insertion of a tripeptide spacer between the hapten and carrier resulted ... | 1978 | 77841 |
| structure and orientation of cytochrome c oxidase in crystalline membranes. studies by electron microscopy and by labeling with subunit-specific antibodies. | the structure and the orientation of cytochrome c oxidase molecules in crystalline cytochrome c oxidase membranes (vanderkooi, g., senior, a.e., capaldi, r.a., and hayashi, h. (1972) biochim. biophys. acta 274, 38-48) were studied by image analysis of electron micrographs and by reacting the crystalline preparations with immune gamma-globulins against individual cytochrome c oxidase subunits. binding of gamma-globulins to the membranes was detected by the following two methods: (a) electrophoret ... | 1978 | 77860 |
| electron microscope studies of ph effects on assembly of tubulin free of associated proteins. delineation of substructure by tannic acid staining. | bovine brain tubulin, purified by phosphocellulose chromatography (pc), was assembled in the presence of 10% dimethyl sulfoxide (dmso), and the reaction was monitored turbidimetrically. samples were fixed in glutaraldehyde-tannic acid after completion of polymerization, as indicated by no further change in absorbance, and then sectioned and studied electron microscopy, with special attention being given to the arrangement of protofilaments in the walls of formed elements. samples of pc-tubulin w ... | 1978 | 77862 |
| cellular localization of epidermal transglutaminase: a histochemical and immunochemical study. | | 1978 | 77870 |
| the effect of human serum on 3h-thymidine incorporation in human prostate tumors in tissue culture. | human prostatic tumors, 4 adenomas and 4 adenocarcinomas, were established in tissue culture. we used the incorporation of 3h-thymidine into deoxyribonucleic acid as a measure of cell activity to compare the effects of supplementing the culture media with human or fetal calf serum. 3h-thymidine incorporation was greater with human serum, with up to 120 per cent increase observed. the addition of dihydrotestosterone (10(-8)m.) did not enhance or inhibit 3h-thymidine incorporation. | 1978 | 77919 |
| infant's utilisation of his milk intake. | | 1975 | 78177 |
| antibodies to carbohydrates: preparation of antigens by coupling carbohydrates to proteins by reductive amination with cyanoborohydride. | | 1978 | 78431 |
| [immunochemical comparison of tryptophanyl-trna-synthetases]. | a fraction of immunoglobulins was isolated from the sera of rabbit immunised by a homogeneous beef pancreas tryptophanyl-trna-synthetase (trsase). the igg fraction was shown to inhibit the enzymatic activity during aminoacylation of yeast trnatrp and tryptophan activation. by using the radioimmunoadsorption technique, the interaction of igg was tested with trsaes from beef pancreas and identical enzymes from other sources (contained in the total preparation). beef liver trsase efficiently inhibi ... | 1978 | 78439 |
| a new approach to the structural determination of glycoproteins and polysaccharides: anhydrous hf solvolysis. | from experiments with glycoproteins containing the glycopeptide linkages, arabinose-o-hydroxyproline and galactose-o-serine (plant cell wall glycopeptides), n-acetylgalactosamine-o-serine/threonine (pig submaxillary mucin), and n-acetyl-glucosamine-n-asparagine (fetuin), it is apparent that anhydrous liquid hf, a reagent commonly used by snythetic peptide chemists for the complete removal of protecting groups from synthetic peptides, cleaves the o-glycosidic linkages of neutral sugars in 1 hr at ... | 1978 | 78502 |
| [use of chlormadinone acetate (cap) in bovine reproduction (author's transl)]. | the results obtained with chlormadinone acetate (cap) in the treatment of dairy cattle with inactive ovaries (anoestrus) and during the puerperium are reported. cap was administered to eighteen animals with inactive ovaries for fourteen days. oestrus occurred in fourteen animals within from one to four days after treatment had been completed. insemination during this oestrus resulted in pregnancy in five animals. cap was also administered to sixty animals on three farms for fourteen days during ... | 1978 | 78531 |
| [therapeutic effect of myelin basic protein and synthetic encephalitogenic peptide in guinea pigs with experimental allergic encephalomyelitis]. | effect of antigenic factors of experimental allergic encephalomyelitis (alkaline protein of myelin and synthetic encephalitogenic peptide) was studied in guinea pigs with distinct manifestations of the experimental allergic encephalomyelitis. the animals recovered after prolonged administration of alkaline protein of myelin within 14 days, if they were sensibilized either by bovine alkaline protein of myelin or by synthetic encephalitogenic peptide. synthetic encephalitogenic peptide was only ef ... | 1978 | 78577 |
| [isolation and analysis of encephalitogenic fractions of spinal cord proteins]. | main encephalitogenic protein was isolated from bovine medulla spinalis and separated into homogenous polypeptide fractions by gel filtration; some of these fractions had the high encephalitogenic activity. the main protein and its fractions, possessing the encephalitogenic activity, caused a development of cross-reacting antibodies and a sensitization to intracutaneous administration of encephalitogenic preparations in animals. non-encephalitogenic main protein sensitized animals only to this p ... | 1978 | 78578 |
| the leukocyte migration test in nickel contact dermatitis. | ten patients with contact dermatitis to nickel, confirmed by patch testing, were investigated by the leukocyte migration test. specific inhibition of leukocyte migration was obtained with nickel protein complexes. bovine albumin, human albumin and human epidermis protein were used as carrier proteins in the complexes. specific inhibition of leukocytes from patients with positive patch test to nickel could not be demonstrated with the hapten alone. | 1976 | 78623 |
| synthetic disaccharide-protein antigen for production of specific o2 antiserum for immunofluorescence diagnosis of salmonella. | antisera from rabbits immunized with the synthetic disaccharide paratose 1 leads to alpha 3 mannose, representive of salmonella o-antigen 2, covalently linked to bovine serum albumin (bsa), were used in indirect immunofluorescence studies for the identification of salmonella serogroup a (o-antigen 1,2,12) bacteria. among 1311 enteric bacteria tested, 497 were salmonella. the anti-paratose 1 leads to alpha 3 mannose-bsa serum identified correctly all the 63 serogroup a strains tested. no positive ... | 1978 | 78659 |
| [histological studies on the hypothalamic adenohypophyseal-gonadal system after mestranol and chlormadinone acetate administration in fattening bulls]. | seventy-six young steers were examined to elucidate the action of the steroid oestrogen mestranol and the gestagen chloromadinone acetate upon the morphology of hypothalamus, adenohypophysis, testes, and accessory sexual glands. the doses used were those applicable also to real animal husbandry. the mechanisms of action of the above pharmaceutical products are discussed against the background of the experimental results. no pathological alteration was observed that might prohibit application of ... | 1978 | 78694 |
| separation and characterization of two bovine alpha1-fetoprotein molecular variants by concanavalin a-sepharose chromatography. | | 1978 | 78710 |
| immunologic approaches toward detection of type c viral expression in man. | type c rna viruses have been isolated from a large number of mammalian species. these agents may be horizontally transmitted as infectious cancer-inducing agents, or vertically transmitted from one generation to the next, often in an unexpressed form, within the host genome. to date, the translational products of three viral genes have been identified. with purified virus-coded proteins as probes, sensitive and highly specific radioimmunologic assays have been developed for the detection of anti ... | 1978 | 78714 |
| the temperature dependence of the surface tension of aqueous solutions of plasma proteins. | the surface behavior of aqueous solutions of fibrinogen, transferrin, gamma-globulin and albumin at the liquid-gas interface has been investigated by a modified wilhelmy technique. the temperature dependence of the surface tension was studied over a temperature range of 20--80 degrees c and a ph range of 2--12. most pronounced conformational changes of fibrinogen with this technique were found in physiological conditions: 35--45 degrees c and ph 7--8. a conformational change was found for gamma- ... | 1978 | 78726 |
| bovine fetus-specific serum proteins. purification and characterization of alpha1-fetoprotein and immunological identification of alpha2- and beta-fetoproteins. | bovine alpha1-fetoprotein was isolated from fetal calf serum by successive procedures of concanavalin a-sepharose chromatography, deae-sephadex chromatography, sp-sephadex chromatography and preparative disc polyacrylamide gel electrophoresis. the bovine alpha1-fetoprotein preparation was considered homogeneous by several physicochemical and immunochemical criteria. bovine alpha1-fetoprotein has a molecular weight of 68 000 with an amino acid composiotn similar to that of other mammalian alhpa1- ... | 1978 | 78727 |
| hydrodynamic orientation of sperm heads for flow cytometry. | two specially shaped sample injection tubes orient flat-shaped mature sperm heads in a flow cytometer. orientation allows control of a photometric difficulty experienced with some flow cytometers in the measurement of fluorescent and scattered light from sperm and other flat cells. both relative dna-stain content and a measure of cell flatness can now be determined simultaneously for each cell at the high rates possible with flow instruments. | 1978 | 78735 |
| induction of immunological tolerance to the penicilloyl antigenic determinant. i. evaluation of penicilloylated amino acid polymers and copolymers in mice. | antibody responses to the penicilloyl (bpo) group, the major antigenic determinant of penicillin allergy induced in c3h mice by penicilloylated bovine gamma globulin in complete freund's adjuvant, were reduced or abolished by various amino acid polymers and copolymers of different composition and size carrying bpo groups. tolerogenic treatment was effective before or after primary immunization and also during anamnestic responses. the unresponsive state was of long duration and persisted even af ... | 1978 | 78853 |
| induction of immunological tolerance to the penicilloyl antigenic determinant. ii. evaluation of stable and unstable penicilloyl dextrans. | a specific tolerant state to the major antigenic determinant of penicillin allergy, the penicilloyl group, was induced in c3h mice primarily immunized with penicilloylated bovine gamma globulin in complete freund's adjuvant. tolerance was obtained by intraperitoneal administration of either of two penicilloyl-bearing dextrans of molecular weight 2 x 10(6). one conjugate contained penicilloyl groups stably bound via a 1,6-diaminohexane spacer, the other bore the penicilloyl groups directly bound ... | 1978 | 78854 |
| alpha-globulin follicular fluid proteins within small and large bovine follicles. | fluids from small (ff-s) and large (ff-l) bovine ovarian follicles were observed electrophoretically for differences in the alpha-globulin protein profile. ff-l possessed a fast migrating alpha-globulin 3 and a greater percentage of ff-l samples contained a higher number of alpha-globulin bands. | 1978 | 78859 |
| specificity of the blastogenic response of human mononuclear cells to herpesvirus antigens. | peripheral blood mononuclear (pbm) cells from donors with a history of prior infection with herpes simplex virus, varicella-zoster virus, and/or cytomegalovirus were cultured for 2 to 8 days with glycine-extracted antigens prepared from these viruses and from infectious bovine rhinotracheitis virus. the proliferative response of pbm cells from all donors was specific during the first 6 days in culture. during this period the cellular immune responses of the seronegative donors were clearly diffe ... | 1978 | 78901 |
| sources of potential residue problems in dairy herds. | drugs and chemicals provide economic benefit to animal and crop production; to derive this benefit they must be used appropriately. data from the united states department of agriculture, food safety and quality service residue monitoring program indicates certain drugs and chemicals are not being used appropriately by the dairy industry, and residues are occurring in cow carcasses at slaughter. residues of antibiotics and certain pesticides which are administered intentionally are being found. d ... | 1978 | 78934 |
| characterization of the proteolytic fragments of bovine colostral igg1. | bovine colostral igg1 was subjected to both papain and pepsin hydrolysis. papain digestion appeared to be optimal at ph 7.4 in the presence of 0.01 m cysteine. the molecule was split at the cooh-terminal side of the interchain disulfide bond(s), and in addition to fab fragments, two fc fragments, designated fc(i) and fc(ii), were obtained. both fc fragments had an identical nh2-terminal sequence, but differed in m.w. by about 10,000, with fc(ii) being the smaller one. differences were also obser ... | 1978 | 78949 |
| technical improvements in the mixed antiglobulin rosetting reaction with consequent demonstration of high numbers of immunoglobulin-bearing lymphocytes in viable preparations of human peripheral blood. | a modification of the mixed antiglobulin rosetting reaction (marr) to improve sensitivity as a test for immunoglobulin (ig)-bearing human blood lymphocytes is described. a mean 5.7%, lymphocytes ig-positive by the marr when rosettes were formed in medium containing 0.2% bovine serum albumin (bsa), increased to 20% when rosettes were formed in 5% bsa or by incubating the lymphocytes or indicator erythocytes with vibrio cholerae neuraminidase before rosetting. under these various rosetting conditi ... | 1978 | 78951 |
| identification of a common antigen of herpes simplex virus bovine herpes mammillitis virus, and b virus. | in immunoelectrophoretic analyses one common antigen was demonstrated in antigen preparations from herpes simplex virus types 1- and 2- (hsv-1 and hsv-2), bovine herpes mammillitis (bhm) virus-, and b virus-infected cells solubilized by triton x-100. the antigen was also demonstrated in solubilized purified hsv-1 and bhm virus. the common antigen was identified as antigen 11 of hsv-1 or hsv-2. differences were found in the polypeptide composition of the related antigens when isolated from the fo ... | 1978 | 78988 |
| [d-xylose test with preliminary activation of protein, fat and carbohydrates]. | | 1978 | 79013 |
| [increasing the sensitivity of the radioimmunological determination of thyrotropin by means of blood plasma concentration]. | | 1978 | 79016 |
| dopamine and schizophrenia. a therapy revisited and revised. | evidence is presented for a modulatory role for dopamine in the c.n.s. and for an indirect rather than a direct involvement of dopaminergic systems in the production of schizophrenic symptoms. the fine structure of dopaminergic fibres indicates that a majority of presynaptic boutons are not in close juxtaposition with postsynaptic neuronal membranes. this points to a wider distribution of receptors than localisation to dense postsynaptic membrane. preliminary studies of receptor localisation sup ... | 1978 | 79086 |
| auer rods: another sacred cow? | | 1978 | 79120 |
| anaphylaxis in isolated rabbit lungs. | lungs from rabbits sensitized to ovalbumin or bovine gamma-globulin were isolated and perfused with autologous blood. the response to antigen challenge via the perfusate was immunologically specific and characterized by a marked increase in perfusion resistance, a moderate increase in airway resistance and a small decrease in lung compliance. the response could also be elicited by specific antigen challenge in lungs from sensitized rabbits perfused with blood from normal rabbits and in lungs fro ... | 1978 | 79201 |
| [complexity of antigen recognition]. | | 1978 | 79228 |
| xenoantibody directed against molecular components of the hla system. quantitation of antibody to serologically defined hla complexes on the cell surface. | a quantitative absorption-blocking assay was used to measure the levels of antibody in immune rabbit sera that react with the serologically defined hla molecular complex on the cell surface. quantitation was determined by comparing the number of xenoantibody-treated cells with the number of untreated control cells needed to absorb a defined amount of cytolytic alloantibody. complement-dependent cytolytic titers were used to standardize the total antibody content reactive with cell surface antige ... | 1978 | 79250 |
| dealing with data: the practical use of numerical information--(3) data collection and display. | | 1978 | 79263 |
| studies on the immune response in chickens ii. functions of carrier-reactive cells in anti-hapten responses. | | 1978 | 79270 |
| inhibitory effects of imidazolines on histamine liberation from human leukocytes and on tracheal smooth muscle tone. | antigen-induced ige-mediated release of histamine from human leukocytes, an in vitro model of allergic reactions, was blocked by imidazole and imidazole-compounds such as oxymetazoline and clonidine. the h-2-antihistamines antagonized this effect of imidazolines. alpha- and h-1-receptor blocking agents did not antagonize the effect. the contractile effects of the imidazolines were tested on tracheal preparations from the cow and guinea-pig. imidazole was found to be a rather potent contracting a ... | 1978 | 79298 |
| [detection of cell-mediated immunity against protein antigen by the macrophage adherence inhibition test (author's transl)]. | the macrophage adherence inhibition test is a suitable assay for detection of cell-mediated immunity against bovine gamma globulin (bgg) in guinea pigs. the test was performed with peritoneal cells (pc) in hemocytometer chambers. 10 mg/ml bgg were added as antigen. the washing step was performed with a burette. an average standard deviation of 5% was obtained with this washing method. day-to -day-investigations in individual animals show differences with respect to the degree of cell-mediated im ... | 1978 | 79307 |
| the induction of immunologic tolerance with type iii pneumococcal polysaccharide cross-linked or coupled to protein. | type iii pneumococcal polysaccharide molecules were linked to one another, i. e. cross-linked. the product was essentially insoluble in saline. large pieces weighing 1-2 mg were inserted into mice. they produced immunologic paralysis or immune tolerance. when placed intraperitoneally in millipore chambers the cross-linked polysaccharide did not have any apparent effect, for the mice were not tolerized. when pneumococcal polysaccharide was linked to a protein, the amount of polysaccharide require ... | 1978 | 79330 |
| subfractionation of a supernatant from bovine thyroid [proceedings]. | | 1977 | 79354 |
| human thyrotropin and its alpha and beta subunits. | a new procedure is described for the isolation of human thyrotropin using ion exchange chromatography and gel filtration only. thyroid stimulating activity of the final preparation of our human thyrotropin amounted to 0.5 iu/mg by bioassay. the alpha and beta subunit of the hormone were also obtained by a new procedure. in this method the native hormone was incubated in an acidified 8 m urea solution and the chains were then separated by ion exchange chromatography and gel filtration. the amino- ... | 1977 | 79370 |
| phospholipase a1 from bovine thyroid lysosomes [proceedings]. | | 1977 | 79387 |
| [peroxidase, a marker enzyme for granular endoplasmic reticulum in thyroid gland (proceedings)]. | | 1977 | 79388 |
| biosynthesis of bovine leukemia virus (blv) major internal protein (p24) in infected cells and x. laevis oocytes microinjected with blv 60-70s rna [proceedings]. | | 1977 | 79394 |
| distribution of bovine leukemia virus proviral dna sequences in tissues of animals with enzootic bovine leukosis [proceedings]. | | 1977 | 79401 |
| on the mechanism of action of adjuvants. | evidence is presented that one a way which adjuvants exert their effect is by initiating an enduring increase in the localization of labelled cells in draining lymphoid tissues. agents not generally considered to be adjuvants (carbon, latex, sheep erythrocytes) but capable of altering lymphocyte recirculation are shown to prevent the induction of tolerance by soluble bovine gamma globulin (bgg). these properties fulfil the criteria for adjuvanticity as defined by dresser (1968) and link the expr ... | 1978 | 79548 |
| subclass restriction of murine anti-carbohydrate antibodies. | examination of the subclass distribution of murine antibodies directed against groups a and c streptococcal carbohydrate, alpha-(1 leads to 3) dextran and phosphocholine yields the surprising observation that these carbohydrate antigens stimulate igg responses largely restricted to the rare igg3 subclass. this subclass restriction is particularly impressive in light of the low circulating levels of igg3 in nonimmune mouse serum and the failure of a variety of other antigens including proteins an ... | 1978 | 79606 |
| antigen-specific t cell-mediated suppression. ii. in vitro induction by i-j-coded l-glutamic acid50-l-tyrosine50 (gt)-specific t cell suppressor factor (gt-t8f) of suppressor t cells (t82) bearing distinct i-j determinants. | the induction of new suppressor t cells (ts2) by suppressive extracts (tsf) from l-glutamic acid50l-tyrosine50 (gt) nonresponder mice was examined. incubation of normal spleen cells with allogeneic gt-tsf for 2 days in vitro led to the generation of ts2 cells able to suppress subsequent responses to the immunogen gt-methylated bovine serum albumin (gt-mbsa) in vivo. this induction occurred efficiently when tsf donor and target cells differed at all of h-2, including the i-j subregion. b10.br (h- ... | 1978 | 79609 |
| definition and synthesis of the essential amino acid sequence for experimental allergic encephalomyelitis in lewis rats. | | 1978 | 79612 |
| demonstration of a cytoplasmic receptor protein for oestrogen in the canine prostate gland. | a receptor protein that selectively binds oestrogens has been demonstrated in the cytosol of the canine prostate gland. the steroid--receptor complex was found to have a sedimentation coefficient of 4--5 s with respect to bovine serum albumin after sucrose density-gradient centrifugation. the high affinity and low capacity of the protein for oestrogens was indicated by displacement studies, which gave a value of 3.8 +/- 1.53 (s.d.) x 10(-10) mol/l for the dissociation constant. a metastasizing p ... | 1978 | 79630 |
| the immune response of allophenic mice to 2,4-dinitrophenyl (dnp)-bovine gamma globulin. i. allotype analysis of anti-dnp antibody. | the question of whether or not lymphoid cells can cooperate across a histocompatibility difference barrier has been studied in several laboratories. using an adoptive transfer system, katz et al. (1) first showed that t cells from (low responder x high responder) f(1) mice, primed to the terpolymer l-glutamic acid, l-lysine, l-tyrosine (glt), could collaborate with 2,4-dinitrophenyl (dnp)-primed b cells from a high responder, but not a low responder strain, in response to dnp-glt. the response t ... | 1978 | 79635 |
| partial purification and some properties of inhibitors to the hemagglutinating activity of leukocytosis-promoting factor (lpf) from bordetella pertussis [proceedings]. | | 1978 | 79665 |
| parathyroid secretory protein: immunocytochemical localisation within cells that contain parathyroid hormone. | | 1978 | 79733 |
| multifactorial cancer. | | 1978 | 79769 |
| cow's milk as a cause of infantile colic in breast-fed infants. | 18 mothers of 19 breast-fed infants with infantile colic were put on a diet free of cow's-milk protein. the colic disappeared promptly from 13; in 12, it reappeared on at least two further indirect challenges (in the form of a diet containing cow's milk to the mother). most infants became symptom-free at age 2 to 4 months; at 4 months, only 4 reacted with colic when the mother took cow's milk. 5 infants were directly challenged with cow's milk; 4 reacted promptly with colic. other signs of intol ... | 1978 | 79803 |
| [study of nucleic acid structure by immunochemical methods. i. antibodies specific for 6-sulfo-5,6-dihydro-4-methoxyaminopyrimidinone-2]. | immunization of animals with dna modified by a mixture of bisulphite and o-methylhydroxylamine and methylated bovine serum albumin results in production of antibodies mainly reacting with modified dna. antibodies that react with denatured dna were produced in minute quantity. it was shown that elicited antibodies possess a high specificity and have the ability to recognize only nucleotides with a double modification. the immune sera were fractionated by sephadex g-200 column chromatography and t ... | 1978 | 79979 |
| separation of anti-dinitrophenyl antibodies according to affinity by fractionated elution from immunoadsorbent using thiocyanate. | the possibility of fractionating anti-dnp-antibodies according to their affinity by successive elution from a dnp-immunoadsorbent with increasing concentrations of thiocyanate (kscn) was investigated. using kscn at molarities of 0.5 m, 2.0 m and 3.0 m in a stepwise elution procedure, the mean affinity of the eluted antibody fractions was found to increase with increasing molarity of the eluent. the method described is a simple, reliable means of separating antibodies on an affinity basis which i ... | 1978 | 80019 |
| active enhancement in the primate by bone marrow i. ia-like determinants on nucleated bone marrow cells. | this study investigates the possibility that the active enhancing properties of bone marrow (bm) cells may be related to ia-like determinants on their surface membranes. less chromium was released on a cell per cell basis from nucleated baboon bm cells used as targets in complement-dependent cytotoxicity than from labelled lymph node lymphocytes. on the other hand, bm cells stimulated allogeneic lymphocytes in culture more vigorously than lymph node lymphocytes. bm cells with lymphocyte-activati ... | 1978 | 80050 |
| [induction of immune tolerance to protein antigens in mice by 6-mercaptopurine (author's transl)]. | the tolerance induction in mice to human serum albumin (hsa) and bovine gamma globulin (bgg) by combined injections of antigen and 6-mercaptopurine is studied. after pretreatment with antigen and mercaptopurine specific unresponsiveness or hyporesponsiveness are observed both in inbred (cba) and outbred mice. under the experimental protocol hsa is more tolerogenic than bgg. in cba-mice tolerance to bgg was only found on the level of igg-antibodies. the factors which influence the development of ... | 1978 | 80124 |
| heparin-related immune thrombocytopenia. studies of antibody-heparin specificity. | | 1978 | 80131 |
| the chemical mediation of delayed hypersensitivity skin reaction. ii. characterization of a macrophage-chemotactic factor from bovine gamma-globulin-induced skin reaction in guinea pigs. | macrophage-chemotactic factors were extracted from delayed hypersensitivity skin lesions induced by bovine gamma-globulin in guinea pigs. the most active factor, mcfs--1, was highly purified and found to be a heat-labile protein with a molecular weight of 150,000 and to possess in vivo as well as in vitro activity. this factor was homogeneous during polyacrylamide gel electrophoresis, and the chemotactic activity was associated exclusively with this band. further characterization revealed that i ... | 1978 | 80137 |
| [effects of tsh on thyroid ribonucleic acids (author's transl)]. | bovine thyroid slices were incubated with tsh (20 mu/ml) in the presence of 0.04 microgram/ml actinomycin d. the incorporation of 3h adenosine or 3h uridine in the microsomal rna fraction was studied. tsh enhanced the labeling of this rna. after gel electrophoresis in 100% formamide, the distribution of the radioactivity showed a relatively larger incorporation of the precursors in the region corresponding to heavy molecular weight rna (3 x 10(6) daltons). these data suggest that tsh could exert ... | 1978 | 80151 |
| [isolation and characterization of beef thyroid giant rna]. | total hmw-rnas were prepared by three different methods (method with phenol, method with naclo4, method without phenol using ultrogel aca 22 filtration). giant rnas were obtained in the void volume by filtration on sepharose 2b. the giant rnas/total hmw-rna ratio is higher (6.77%) with the gel filtration method than with phenol or naclo4 methods (1.41% and 1.00% respectively). the nucleotide composition of these rnas is dna-like and the sedimentation constants are approximately 70-100 s. | 1978 | 80168 |
| subcellular structure of bovine thyroid gland. vii. a study on the distribution of bovine thyroid plasma membranes by density gradient centrifugation in zonal rotors. | in order to obtain plasma membrane-rich fractions two methods were tried. approach a was based on differential pelleting followed by discontinous gradient centrifugation in a b-xiv zonal rotor. in approach b homogeneization was performed in buffered water (nahco3, ph 7.4). the 73 300 x g pellet from this homogenate was subjected to buoyant density equilibrium in a hs zonal rotor (continuous sucrose gradient). using approach a, the highest relative specific activity for plasma membrane markers wa ... | 1978 | 80197 |
| [puerperal prevention in cattle using oral cap]. | | 1978 | 80216 |
| chromatographic behavior of cyclic 3',5'-nucleotide phosphodiesterases on columns of immobilized inhibitors. | | 1978 | 80229 |
| clostridiopeptidase b inhibition by plasma marcroglobulins and microbial antiproteases. | clostridiopeptidase b (ec 3.4.22.8) was not inhibited by stoichiometric amounts of lima bean trypsin inhibitor, ovomucoid trypsin inhibitor, kuntiz bovine trypsin inhibotor, kunitz soybean trypsin inhibitor or ovoinhibitor. activity was diminished at relatively high concentrations of the three latter inhibitors. human plasma alpha 2-macroglobulin inhibited both the amidase and protease activity of the enzyme. rat and dog plasmas contained high molecular weight inhibitors, presumably macroglobuli ... | 1978 | 80231 |
| structure of antigenic determinants in the amino-terminal region of bovine fibrinogen aalpha chain. | | 1978 | 80317 |
| immunogenetics of a macroglobulin allotype in cattle. | | 1978 | 80347 |
| new surface-associated heat-labile colonization factor antigen (cfa/ii) produced by enterotoxigenic escherichia coli of serogroups o6 and o8. | enterotoxigenic escherichia coli (etec) belonging to serogroups o6 and o8 do not possess the h-10407-type colonization factor antigen (cfa/i). however, these frequently isolated etec were found to possess a second and distinct heat-labile surface-associated colonization factor antigen, termed cfa/ii. whereas cfa/i mediates mannose-resistant hemagglutination of human group a erythrocytes, cfa/ii does not. cfa/ii mediates mannose-resistant hemagglutination of bovine erythrocytes, and mannose-resis ... | 1978 | 80383 |
| concanavalin a-peroxidase-diaminobenzidine-periodic acid-m-aminophenol-fast black salt k: a method for the dual staining of neutral complex carbohydrates. | a method has been developed for the dual staining of neutral complex carbohydrates in light microscopy. it combines a concanavalin a-peroxidase-diaminobenzidine (con a-po-dab) method with a period acid-m-aminophenol-fast black salt k (pa-ap-fbk) sequence. with the combined method it is possible to stain alpha-d-glycosyl and alpha-d-mannosyl residues brown and 1,2-glycol groups of neutral complex carbohydrates blackish purple. the validity of the method has been confirmed with appropriate histoch ... | 1978 | 80396 |
| quantitation of endotoxin in cell-free rumen fluid of cattle. | | 1978 | 80404 |
| immunohistochemical detection of a cross-reacting virus antigen in mouse mammary tumors and human breast carcinomas. | an indirect immunoperoxidase method was first used to localize mouse mammary tumor virus (mmtv) antigens in paraffin sections of mammary tumors of paris riii and cd8f1 mice. by using the same method, an antigen with cross-reactivity to a group-specific antigen (gp52, a 52,000 dalton glycoprotein) of mmtv was detected in paraffin sections of human breast carcinomas. the specificity of this reaction with antibody against mmtv was examined by absorption of the igg with: a) purified gp52; b) several ... | 1978 | 80418 |
| palladium chloride as a stain for elastin at the ultrastructural level. | palladium chloride in aqueous solution stains elastic fibers in thin sections of epon-embedded tissues. when palladium chloride is used with a lead citrate counterstain, high contrast sections with gray to black elastic fibers are obtained. the stain was tested on newborn and adult mammalian tissues and on adult tissues from lower animals. sections were mounted on stainless steel grids, stained with 1% palladium chloride solution for 5 to 15 min, rinsed thoroughly, and counterstained with lead c ... | 1978 | 80419 |
| ige antibodies to cow's milk in infants fed breast milk and milk formulae. | | 1978 | 80542 |
| breast-milk monitoring to measure michigan's contamination with polybrominated biphenyls. | in 1973 and 1974, several thousand michigan dairy farms were contaminated by polybrominated biphenyls (p.b.b.) as the result of an industrial accident. an unknown quantity of contaminated meat and dairy products entered the food chain before contaminated farms were quarantined. to determine the extent of human exposure, p.b.b. concentrations were measured in human breast milk, which was collected in a random-sample survey from nursing mothers throughout michigan. 96% of 53 samples from michigan' ... | 1978 | 80575 |