| promotion of growth and cu accumulation of bio-energy crop (zea mays) by bacteria: implications for energy plant biomass production and phytoremediation. | three metal-resistant and plant growth-promoting bacteria (burkholderia sp. gl12, bacillus megaterium jl35 and sphingomonas sp. ym22) were evaluated for their potential to solubilize cu(2) (oh)(2)co(3) in solution culture and their plant growth promotion and cu uptake in maize (zea mays, an energy crop) grown in a natural highly cu-contaminated soil. the impacts of the bacteria on the cu availability and the bacterial community in rhizosphere soils of maize were also investigated. inductively co ... | 2012 | 22459071 |
| isolation and characterization of dimethyl sulfide (dms)-degrading bacteria from soil and biofilter treating waste gas containing dms from the laboratory and pulp and paper industry. | dimethyl sulfide (dms) is one of the sulfurous pollutants present in the waste gas generated from the pulp and paper industry. dms has environmental health implications; therefore, it is necessary to treat the waste gas containing dms prior to discharge into the environment. a bench-scale biofilter was operated in the laboratory as well as in a pulp and paper industry for the treatment of dms. both the biofilters were packed with pre-sterilized wood chips and cow dung/compost of the same origin ... | 2012 | 22447220 |
| biodegradation of bensulfuron-methyl and its effect on bacterial community in paddy soils. | bensulfuron-methyl (bsm) is a new kind of sulfonylurea herbicide widely used to control broad-leaf weeds in rice paddies. the aim of this work was to study bsm biodegradation in paddy soils with bsm-degrading bacteria bacillus megaterium l1 and brevibacterium sp. bh and its effect on the structures of soil bacterial community. more than 90 % of bsm could be degraded in paddy soils with 0.0355 mg kg⁻¹ bsm concentration. addition of bsm-degrading bacterial strains bacillus megaterium l1 into bsm c ... | 2012 | 22430059 |
| autodisplay of functional cyp106a2 in escherichia coli. | cytochrome p450 enzymes catalyse a wide variety of reactions, including the hydroxylation and epoxidation of cc bonds, and dealkylation reactions. there is high interest in these reactions for biotechnology and pharmaceutical processes. many p450s require membrane surroundings and have substrates that do not cross biological membranes. to circumvent these obstacles, cyp106a2 from bacillus megaterium was expressed on the outer membrane of escherichia coli cells by autodisplay. exposure on the sur ... | 2012 | 22426093 |
| extra ef hand unit (dx) mediated stabilization and calcium independency of α-amylase. | it is the common feature of α-amylases that calcium ion is required for their structural integrity and thermal stability. all amylases have at least one ca(2+) per molecule; therefore amino acids involved in calcium binding are specific and conserved. in this study, sequence analysis revealed the presence of ef-hand-like motif in calcium-binding loop of bacillus megaterium who (bmw)-amylase that was previously isolated from bmw. the ef-hand motif and its variants (ef-hand-like motif) are the mos ... | 2013 | 22407721 |
| macin family of antimicrobial proteins combines antimicrobial and nerve repair activities. | the tertiary structures of theromacin and neuromacin confirmed the macin protein family as a self-contained family of antimicrobial proteins within the superfamily of scorpion toxin-like proteins. the macins, which also comprise hydramacin-1, are antimicrobially active against gram-positive and gram-negative bacteria. despite high sequence identity, the three proteins showed distinct differences with respect to their biological activity. neuromacin exhibited a significantly stronger capacity to ... | 2012 | 22396551 |
| the crystal structure of the fad/nadph-binding domain of flavocytochrome p450 bm3. | we report the crystal structure of the fad/nadph-binding domain (fad domain) of the biotechnologically important bacillus megaterium flavocytochrome p450 bm3, the last domain of the enzyme to be structurally resolved. the structure was solved in both the absence and presence of the ligand nadp(+), identifying important protein interactions with the nadph 2'-phosphate that helps to dictate specificity for nadph over nadh, and involving residues tyr974, arg966, lys972 and ser965. the trp1046 side ... | 2012 | 22356131 |
| direct and mediated electrochemical response of the cytochrome p450 106a2 from bacillus megaterium atcc 13368. | cyp106a2 is one of only a few known steroid hydroxylases of bacterial origin, which might be interesting for biotechnological applications. despite the enzyme having been studied for more than 30 years, its physiological function remains elusive. to date, there have been no reports of the redox potential of cyp106a2, which was supposed to be unusually low for a cytochrome p450. in this work we show that cyclic voltammetry is not only suitable to determine the redox potential of challenging prote ... | 2012 | 22342191 |
| identification of mutant asp251gly/gln307his of cytochrome p450 bm3 for the generation of metabolites of diclofenac, ibuprofen and tolbutamide. | the soluble, catalytically self-sufficient cytochrome p450 bm3 from bacillus megaterium is a good candidate as biocatalyst for the synthesis of drug metabolites. to this end, error-prone polymerase chain reaction (pcr) was used to generate a library of p450 bm3 mutants with novel activities toward drugs. the double mutant asp251gly/gln307his (a2) with activities towards diclofenac, ibuprofen and tolbutamide was identified by screening with the alkali method. this is based on the detection of nad ... | 2012 | 22337118 |
| hydroxylation of the triterpenoid dipterocarpol with cyp106a2 from bacillus megaterium. | the bacterial steroid-hydroxylase cyp106a2 from bacillus megaterium atcc 13368 hydroxylates a variety of 3-oxo-δ-4-steroids and has recently been shown to catalyse regioselective hydroxylation of the diterpene abietic acid, as well as the pentacyclic triterpene 11-keto-β-boswellic acid. the broad substrate spectrum of this enzyme makes it an excellent candidate for biotechnological application. because the natural substrate of this enzyme is not known, we assumed that the whole substrate spectru ... | 2012 | 22269065 |
| debottlenecking recombinant protein production in bacillus megaterium under large-scale conditions--targeted precursor feeding designed from metabolomics. | in the present work the impact of large production scale was investigated for bacillus megaterium expressing green fluorescent protein (gfp). specifically designed scale-down studies, mimicking the intermittent and continuous nutrient supply of large- and small-scale processes, were carried out for this purpose. the recombinant strain revealed a 40% reduced gfp yield for the large-scale conditions. in line with extended carbon loss via formation of acetate and carbon dioxide, this indicated obvi ... | 2012 | 22252649 |
| impact of rock materials and biofertilizations on p and k availability for maize (zea maize) under calcareous soil conditions. | the present work evaluated the synergistic effects of soil fertilization with rock p and k materials and co-inoculation with p and k-dissolving bacteria [pdb (bacillus megaterium var. phosphaticum) and kdb (bacillus mucilaginosus and b. subtilis)] on the improvement of p and k uptake, p and k availability and growth of maize plant grown under limited p and k soil conditions (calcareous soil). the experiment was establishment with eight treatments: without rock p and k materials or bacteria inocu ... | 2012 | 23961162 |
| the nematicidal effect of some bacterial biofertilizers on meloidogyne incognita in sandy soil. | in a greenhouse experiment, the nematicidal effect of some bacterial biofertilizers including the nitrogen fixing bacteria (nfb) paenibacillus polymyxa (four strains), the phosphate solubilizing bacteria (psb) bacillus megaterium (three strains) and the potassium solubilizing bacteria (ksb) b. circulans (three strains) were evaluated individually on tomato plants infested with the root-knot nematode meloidogyne incognita in potted sandy soil. comparing with the uninoculated nematode-infested con ... | 2011 | 24031611 |
| cyclodextrin glucosyltransferase production by bacillus megaterium ncr: evaluation and optimization of culture conditions using factorial design. | statistically-based experimental designs were used to optimize the production of cyclodextrin glucosyltransferase (cgtase) from a local isolate of bacillus megaterium using shack culture fermentation. seven cultural conditions were examined for enzyme production and specific activity using plackett-burman factorial design. fermentation time and k(2)hpo(4) level were the crucial for factors improving enzyme production process. the steepest ascent design was adopted-based on the results recorded w ... | 2010 | 23100845 |
| sporulation and regeneration efficiency of phosphobacteria (bacillus megaterium var phosphaticum). | sporulation in bacillus megaterium var phosphaticum (pb - 1) was induced using modified nutrient media. this modified medium induced sporulation within 36 h. after spore induction the spores were kept under refrigerated (5°c) and room temperature (32°c) for five months and survival of spores was studied at 15 days intervals by plating them in nutrient agar medium. it was observed that there was not much variation in the storage temperature (5°c & 32°c). the spore cells of bacillus megaterium var ... | 2007 | 23100674 |
| purification and properties of nad(+)-dependent sorbitol dehydrogenase from bacillus fructosus. | sorbitol dehydrogenase (ec 1.1.1.14), which catalyzes the nad(+)-linked interconversion of d-sorbitol and d-fructose, was purified and crystallized from cell-free extracts of bacillus fructosus grown on d-sorbitol as a sole carbon source. the crystalline enzyme was homogeneous on disc electrophoresis and ultracentrifugation. the molecular weight was 102,000 by the sedimentation equilibrium method. the enzyme acted specifically on d-sorbitol, and showed an optimum ph at 9.0. the k m values for d- ... | 1999 | 27393263 |
| isolation and characterization of a strain of bacillus megaterium that degrades poly(vinyl alcohol). | a poly(vinyl alcohol) (pva)-degrading mixed culture that consisted of sereval microorganisms was obtained from activated sludge from a textile factory. from the culture, a component bacterial strain, bx1, that degraded pva was isolated, and was identified as bacillus megaterium. this strain could degrade pva only in the cocultivation with a bacterial strain, pn19, that was isolated from the same mixed culture. | 1996 | 27299406 |
| saccharification of indigenous starches by β-amylase of bacillus megaterium. | extracellular β-amylase from bacillus megaterium converted indigenous starches from low-grade, raw materials to maltose. the extent of saccharification was higher with gelatinized starches than the raw material. for the gelatinized starches the optima for saccharification were ph 6.9 and 60°c. | 1994 | 24421197 |
| conjugal transfer of enterococcal transposons in bacillus megaterium. | the conjugative enterococcal transposons tn916 and tn919 were introduced into bacillus megaterium by a filtermating technique. the transfer frequencies obtained ranged from 1.3×10(-6) to 6.6×10(-7). the transposons integrated stably into the b. megaterium chromosome. tn916 could generate auxotrophs and was transferred from b. megaterium tn916 transconjugants to other species. | 1994 | 24421193 |
| detection and estimation of aflatoxins using both chemical and biological techniques. | production of aflatoxins on both natural (rice and corn) and semisynthetic (yes) media was conducted using an identified toxin-producing strain ofaspergillus flavus. thea flavus strain was able to produce 4 types of aflatoxins, namely b1, b2, g1, and g2 on rice, corn, and yes media. quantitative data showed that the concentrations of aflatoxins b1 and g1 produced were 52, 40.3, and 39.6; and 64.7, 45.0, and 58.0jug for 50g of rice, corn, and yes media, respectively. in comparison, the yielded am ... | 1993 | 23606067 |
| analysis of the effects of high-let radiations on various biological test objects. ucrl-11387. | | 1964 | 24547293 |
| infectious macromolecules. | | 1962 | 24546764 |
| heterologous expression and biochemical characterization of assimilatory nitrate and nitrite reductase reveals adaption and potential of bacillus megaterium nct-2 in secondary salinization soil. | large accumulation of nitrate in soil has resulted in "salt stress" and soil secondary salinization. bacillus megaterium nct-2 which was isolated from secondary salinization soil showed high capability of nitrate reduction. the genes encoding assimilatory nitrate and nitrite reductase from nct-2 were cloned and over-expressed in escherichia coli. the optimum co-expression condition was obtained with e. coli bl21 (de3) and 0.1mm iptg for 10h when expression was carried out at 20°c and 120rpm in l ... | 2017 | 28389402 |
| a new phthalide from the endophytic fungus xylaria sp. gdg-102. | a new phthalide derivative, xylarphthalide a (1), along with two known compounds (-)-5-carboxylmellein (2) and (-)-5-methylmellein (3), were isolated from the endophytic fungus xylaria sp. gdg-102 cultured from the chinese medicinal plant sophora tonkinensis. their structures were identified by ms and nmr experiments, and the absolute configuration of 1 was further confirmed by single-crystal x-ray diffraction analysis. compound 1-3 showed antibacterial activities against bacillus megaterium, ba ... | 2017 | 28385040 |
| nitrogen fixing potential of various heterotrophic bacillus strains from a tropical estuary and adjacent coastal regions. | in the present study, we report the nitrogen fixing potential of heterotrophic diazotrophs isolated from a tropical estuary and adjacent coastal sea. results of the study revealed that most of the species that are capable of fixing nitrogen in the study area belongs to the genus bacillus. the isolates from the estuary showed maximum homology with bacillus megaterium, b. cereus, b. safencis, b. licheniformis, b. aerophilus, b. oceanisediminis, b. flexus, b. aquimaris, b. vietnamensis, and b. subt ... | 2017 | 28787089 |
| crystal structure and functional characterization of a cytochrome p450 (bacyp106a2) from bacillus sp. pamc 23377. | bacterial cytochrome p450 (cyp) steroid hydroxylases are effectively useful in the pharmaceutical industry for introducing hydroxyl groups to a wide range of steroids. we found a putative cyp steroid hydroxylase (bacyp106a2) from the bacterium bacillus sp. pamc 23377 isolated from kara sea of the arctic ocean, showing 94% sequence similarity with bmcyp106a2 (bacillus megaterium atcc 13368). in this study, soluble bacyp106a2 was overexpressed to evaluate its substrate-binding activity. the substr ... | 2017 | 28633515 |
| in vitro anti-oxidant and anti-microbial potentiality investigation of different fractions of caryota urens leaves. | caryota urens is a member of the arecaceae family and a common plant in the southeast asian region. this plant has been reported as an anti-microbial agent in recent years. thus, we aimed to find out the mic (minimum inhibitory concentration) against different pathogenic microorganism. | 2016 | 28536384 |
| newly identified invertebrate-type lysozyme (splys-i) in mud crab (scylla paramamosain) exhibiting muramidase-deficient antimicrobial activity. | lysozymes are widely distributed immune effectors exerting muramidase activity against the peptidoglycan of the bacterial cell wall to trigger cell lysis. however, some invertebrate-type (i-type) lysozymes deficient of muramidase activity still exhibit antimicrobial activity. to date, the mechanism underlying the antimicrobial effect of muramidase-deficient i-type lysozymes remains unclear. accordingly, this study characterized a novel i-type lysozyme, splys-i, in the mud crab scylla paramamosai ... | 2017 | 28438599 |
| tomato ethylene sensitivity determines interaction with plant growth-promoting bacteria. | plant growth-promoting bacteria (pgpb) are soil micro-organisms able to interact with plants and stimulate their growth, positively affecting plant physiology and development. although ethylene plays a key role in plant growth, little is known about the involvement of ethylene sensitivity in bacterial inoculation effects on plant physiology. thus, the present study was pursued to establish whether ethylene perception is critical for plant-bacteria interaction and growth induction by two differen ... | 2017 | 28586422 |
| antimicrobial properties of silver cations substituted to faujasite mineral. | a goal of our study was to find an alternative to nano-silver-based antimicrobial materials which would contain active silver immobilized in a solid matrix that prevents its migration into the surrounding environment. in this study, we investigated whether silver cations dispersed in an atomic form and trapped in an ion-exchanged zeolite show comparable antimicrobial activity to silver nanoparticles (nps). the biocidal active material was prepared from the sodium form of faujasite type zeolite i ... | 2017 | 28846614 |
| identification of diacetonamine from soybean curd residue as a sporulation-inducing factor toward bacillus spp. | under bioassay-guided investigation, a sporulation-inducing factor (sif) toward bacillus spp. was searched for in methanol (meoh) extracts of soybean curd residues, and diacetonamine (1) was identified as the active compound. sif was first isolated as a monoacetylated derivative (2, 4.1 mg from 655 g soybean curd residues), and its chemical structure was elucidated by field desorption mass spectrometry, electron ionization mass spectrometry, and nuclear magnetic resonance (nmr) analyses. after 4 ... | 2017 | 28545259 |
| bacillus zanthoxyli sp. nov., a novel nematicidal bacterium isolated from chinese red pepper (zanthoxylum bungeanum maxim) leaves in china. | a novel strain, 1433(t), was isolated from leaves of chinese red pepper (huajiao, zanthoxylum bungeanum maxim) collected from gansu province in northwestern china, and was characterised by a polyphasic approach. cells of strain 1433(t) were observed to be gram-stain positive, aerobic, asporogenous, rod shaped, motile and to have peritrichous flagella. the strain was observed to grow at a range of temperatures and ph, 4-45 °c (optimum 28-32 °c) and 6.0-10.0 (optimum ph 6.0-7.0), respectively. gro ... | 2017 | 28550345 |
| levels of l-malate and other low molecular weight metabolites in spores of bacillus species and clostridium difficile. | dormant spores of bacillus species lack atp and nadh and contain notable levels of only a few other common low mol wt energy reserves, including 3-phosphoglyceric acid (3pga), and glutamic acid. recently, bacillus subtilis spores were reported to contain ~ 30 μmol of l-malate/g dry wt, which also could serve as an energy reserve. in present work, l-malate levels were determined in the core of dormant spores of b. subtilis, bacillus cereus, bacillus megaterium and clostridium difficile, using bot ... | 2017 | 28850573 |
| antibacterial cysteine protease from cissus quadrangularis l. | an antibacterial cp was extracted from the stem of cissus quadrangularis and purified with a 5.39 fold increase in specific activity and 8.67% recovery. the molecular weight of the purified enzyme was estimated to be 39kda by sds-page. the purified enzyme appeared as a single band on native-page. the optimum ph and temperature for protease activity were around 6.0 and 50°c respectively. the cp showed ph stability from 3 to 10 and retained more than 90% of its relative protease activity. the addi ... | 2017 | 28539266 |
| bacillus cereus-type polyhydroxyalkanoate biosynthetic gene cluster contains r-specific enoyl-coa hydratase gene. | bacillus cereus and bacillus megaterium both accumulate polyhydroxyalkanoate (pha) but their pha biosynthetic gene (pha) clusters that code for proteins involved in pha biosynthesis are different. namely, a gene encoding maoc-like protein exists in the b. cereus-type pha cluster but not in the b. megaterium-type pha cluster. maoc-like protein has an r-specific enoyl-coa hydratase (r-hydratase) activity and is referred to as phaj when involved in pha metabolism. in this study, the pha cluster of ... | 2017 | 28532241 |
| effects of culture conditions on the size, morphology and wet density of spores of bacillus cereus 569 and bacillus megaterium qm b1551. | the influence of variable culture conditions on the size and wet density of spores of bacillus cereus and bacillus megaterium were examined in this work. culture temperature and initial ph was shown to have a significant impact on the size of both species, with increasingly alkaline culture media and elevated culture temperatures resulting in spores that were, on average, up to 25% reduced in volume. increasing concentrations of inorganic salts in sporulation media exerted differing effects on e ... | 2017 | 28419596 |
| biosurfactant-biopolymer driven microbial enhanced oil recovery (meor) and its optimization by an ann-ga hybrid technique. | a lipopeptide biosurfactant produced by marine bacillus megaterium and a biopolymer produced by thermophilic bacillus licheniformis were tested for their application potential in the enhanced oil recovery. the crude biosurfactant obtained after acid precipitation effectively reduced the surface tension of deionized water from 70.5 to 28.25mn/m and the interfacial tension between lube oil and water from 18.6 to 1.5mn/m at a concentration of 250mgl(-1). the biosurfactant exhibited a maximum emulsi ... | 2017 | 28499818 |
| synthesis of umbelliferone derivatives in escherichia coli and their biological activities. | umbelliferone, also known as 7-hydroxycoumarin, is a phenolic metabolite found in many familiar plants. its derivatives have been shown to have various pharmacological and chemo-preventive effects on human health. a uridine diphosphate glycosyltransferase yjic from bacillus licheniformis dsm 13, a cytochrome p450bm3 (cyp450 bm3) variant namely mutant 13 (m13) from bacillus megaterium, and an o-methyltransferase from streptomyces avermitilis (saomt2) were used for modifications of umbelliferone. | 2017 | 28396694 |
| engineering of versatile redox partner fusions that support monooxygenase activity of functionally diverse cytochrome p450s. | most bacterial cytochrome p450 monooxygenases (p450s or cyps) require two redox partner proteins for activity. to reduce complexity of the redox chain, the bacillus subtilis flavodoxin ykun (y) was fused to the escherichia coli flavodoxin reductase fpr (r), and activity was tuned by placing flexible (ggggs)n or rigid ([e/l]pppp)n linkers (n = 1-5) in between. p-linker constructs typically outperformed their g-linker counterparts, with superior performance of yr-p5, which carries linker ([e/l]ppp ... | 2017 | 28852040 |
| interspecies nutrient extraction and toxin delivery between bacteria. | bacteria have developed various mechanisms by which they sense, interact, and kill other bacteria, in an attempt to outcompete one another and survive. here we show that bacillus subtilis can kill and prey on bacillus megaterium. we find that bacillus subtilis rapidly inhibits bacillus megaterium growth by delivering the trnase toxin wapa. furthermore, utilizing the methionine analogue l-azidohomoalanine as a nutrient reporter, we provide evidence of nutrient extraction from bacillus megaterium ... | 2017 | 28827522 |
| influence of bio-fertilizer containing beneficial fungi and rhizospheric bacteria on health promoting compounds and antioxidant activity of spinacia oleracea l. | this study evaluates the influences of bio fertilizers containing mycorrhizal fungi (glomus fasciculatum, glomus mosseae) individually or in combination with n-fixer (azotobacter chroococcum), k solubilizer (bacillus mucilaginous) and p solubilizer (bacillus megaterium) on soil fertility and phytochemical levels of spinach. | 2017 | 28815474 |
| cyp106a2 - a versatile biocatalyst with high potential for biotechnological production of selectively hydroxylated steroid and terpenoid compounds. | cyp106a2 from bacillus megaterium atcc13368, was identified in the 1970s as one of the first bacterial steroid hydroxylases responsible for the conversion of progesterone to 15β-hydroxyprogesterone. later on it has been proven to be a potent hydroxylase of numerous 3-oxo-δ(4) as well as 3-hydroxy-δ(5)-steroids and has recently also been characterized as a regioselective allylic bacterial diterpene hydroxylase. the main hydroxylation position of cyp106a2 is thought to be influenced by the functio ... | 2017 | 28780179 |
| steroids hydroxylation catalyzed by the monooxygenase mutant 139-3 from bacillus megaterium bm3. | the search of new substrates with pharmaceutical and industrial potential for biocatalysts including cytochrome p450 enzymes is always challenging. cytochrome p450 bm3 mutant 139-3, a versatile biocatalyst, exhibited hydroxylation activities towards fatty acids and alkanes. however, there were limited reports about its hydroxylation activity towards steroids. herein, an escherichia coli-based whole-cell extract containing the recombinant 139-3 protein was used as the biocatalyst to screen 13 ste ... | 2017 | 28752038 |
| role of stereospecific nature of germinants in bacillus megaterium spores germination. | the present study was undertaken with the objective to assess the effect of distinct stereoisomeric forms of nutrient germinants (selected sugars and amino acids) on the process of germination onset in dormant spores of bacillus megaterium mtcc 2949. in this respect, epimers of glucose and enantiomers of alanine were employed in current work. when supplemented with these stereoisomers, spores were found germinated only with d-glucose and d-mannose among epimers of glucose and only with l-alanine ... | 2017 | 28744426 |
| molecular characterization and functional analysis of the hepcidin gene from roughskin sculpin (trachidermus fasciatus). | hepcidin is a kind of cysteine-rich antimicrobial peptide that plays a vital role in host innate immune activity and iron regulation. here, we report the molecular characterization and functional analysis of a novel hamp1 hepcidin isoforms tf-hep from roughskin sculpin, trachidermus fasciatus. a cdna fragment of 988 bp with an orf of 273 bp was obtained. the coding sequence encodes for a signal peptide of 24 amino acids coupled with a prodomain of 40 amino acids and a mature peptide of 26 amino ... | 2017 | 28743631 |
| a single wap domain-containing protein from litopenaeus vannamei possesses antiproteinase activity against subtilisin and antimicrobial activity against ahpnd-inducing vibrio parahaemolyticus. | the single wap domain-containing protein (swd) is a type iii crustin antimicrobial peptide whose function is to defense the host animal against the bacterial infection by means of antimicrobial and antiproteinase activities. a study of swd from litopenaeus vannamei (lvswd) is reported herein about its activities and function against bacteria, particularly the ahpnd-inducing vibrio parahaemolyticus (vpahpnd) that causes acute hepatopancreatic necrosis disease (ahpnd). the lvswd is mainly synthesi ... | 2017 | 28743625 |
| laboratory-scale hydroxylation of steroids by p450bm3 variants. | this chapter describes the asymmetric hydroxylation of steroids on laboratory preparative scale, using engineered variants of p450bm3 (cyp102a1) as enzyme catalyst. the following protocol covers the creation of an escherichia coli bl21-gold (de3) expression strain, including necessary control experiments like plasmid preparation, test expression, and creation of storage cultures, to verify successful experimental access to recombinant expressed p450bm3 variants. the recombinant expressed p450bm3 ... | 2017 | 28710633 |
| application of bacillus megaterium mcr-8 improved phytoextraction and stress alleviation of nickel in vinca rosea. | the current research was performed to evaluate the effect of bacillus megaterium mcr-8 on mitigation of nickel (ni) stress in vinca rosea grown on ni-contaminated soil (50, 100, and 200 mg ni kg(-1) soil). the treated plants exhibited reduced growth, biomass, gas exchange capacity, and chlorophyll (chl) content under ni stress. the inoculated plants growing in ni-contaminated media exhibited relatively higher growth, total soluble protein, and proline contents. similarly, bacterial inoculation i ... | 2017 | 28699781 |
| pegylation of cytochrome p450 enhances its biocatalytic performance for pesticide transformation. | pesticide intoxication is a major public health concern, and unfortunately there is not an effective treatment for severe organophosphorus pesticide intoxication. in this work, a non-immunogenic enzymatic bioconjugate based on cytochrome p450 was assayed for organophosphorus pesticide transformation. enzyme therapy is an alternative approach to inactivate pesticides in the bloodstream, transforming them into less toxic metabolites. a variant of cytochrome p450 (cypbm3 f87a) from bacillus megater ... | 2017 | 28690168 |
| hole hopping through tryptophan in cytochrome p450. | electron-transfer kinetics have been measured in four conjugates of cytochrome p450 with surface-bound ru-photosensitizers. the conjugates are constructed with enzymes from bacillus megaterium (cyp102a1) and sulfolobus acidocaldarius (cyp119). a w96 residue lies in the path between ru and the heme in cyp102a1, whereas h76 is present at the analogous location in cyp119. two additional conjugates have been prepared with (cyp102a1)w96h and (cyp119)h76w mutant enzymes. heme oxidation by photochemica ... | 2017 | 28689401 |
| characterization of bacillus megaterium, bacillus pumilus, and paenibacillus polymyxa isolated from a pinot noir wine from western washington state. | this report provides the first confirmed evidence of bacillus-like bacteria present in a wine from washington state. these bacteria were isolated from a 2013 pinot noir wine whose aroma was sensorially described as being 'dirty' or 'pond scum.' based on physiological traits and genetic sequencing, three bacterial isolates were identified as bacillus megaterium (strain nho-1), bacillus pumilus (strain nho-2), and paenibacillus polymyxa (strain nho-3). these bacteria grew in synthetic media of low ... | 2017 | 28648288 |
| an efficient process for the transformation of betulin to betulinic acid by a strain of bacillus megaterium. | betulinic acid as a derivative of betulin is widely reported for its anti-hiv and antitumor activities. betulin has three most significant positions, i.e., primary hydroxyl group at position c-28, secondary hydroxyl group at position c-3, and alkene moiety at position c-20, where chemical modifications were performed to yield pharmacologically more active derivatives. bioconversion optimization was performed for the enhancement in the percentage of conversion using statistical approach by opting ... | 2017 | 28623491 |
| enhanced degradation of chlorpyrifos in rice (oryza sativa l.) by five strains of endophytic bacteria and their plant growth promotional ability. | endophytic bacteria reside in plant tissues, such as roots, stems, leaves and seeds. most of them can stimulate plant growth or alleviate phytotoxicity of pollutants. there are handful species with dual functions stimulating plant growth and degrading pollutants have been reported. five endophytic bacteria were isolated from chlorpyrifos (cp) treated rice plants and identified as pseudomonas aeruginosa strain rra, bacillus megaterium strain rrb, sphingobacterium siyangensis strain rsa, stenotrop ... | 2017 | 28622646 |
| the role of organic acids on microbial deterioration in the radix pseudostellariae rhizosphere under continuous monoculture regimes. | a three-year field monoculture trial of radix pseudostellariae and complementary laboratory studies were conducted to further elucidate the underlying mechanism responsible for significant decreases in the biomass yield and quality of r. pseudostellariae under continuous monoculture regimes. hplc analysis indicated that continuous monoculture soil was rich in organic acids, which had cumulative effects over time. further analysis suggested that the application of a mixture of organic acids signi ... | 2017 | 28615734 |
| tetranychus urticae mites do not mount an induced immune response against bacteria. | the genome of the spider mite tetranychus urticae, a herbivore, is missing important elements of the canonical drosophila immune pathways necessary to fight bacterial infections. however, it is not known whether spider mites can mount an immune response and survive bacterial infection. in other chelicerates, bacterial infection elicits a response mediated by immune effectors leading to the survival of infected organisms. in t. urticae, infection by either escherichia coli or bacillus megaterium ... | 2017 | 28592670 |
| antimicrobial activity of plant-median synthesized silver nanoparticles against food and agricultural pathogens. | the adjuvant applications of silver nanoparticles (agnps) in food and agro-ecosystems are not completely pursued, which most of the researchers confined to laboratory inventions. agnps is gaining a lot of interest among worldwide researcher due to exhibit antimicrobial properties and unique physiochemical properties. in addition, the facile and economically friendly method called biological method to synthesis of agnps using bryophyllum pinnatum leaf extract over other methods and including anti ... | 2017 | 28583887 |
| impaired coordination of nucleophile and increased hydrophobicity in the +1 subsite shift levansucrase activity towards transfructosylation. | bacterial levansucrases produce β(2,6)-linked levan-type polysaccharides using sucrose or sucrose analogues as donor/acceptor substrates. however, the dominant reaction of bacillus megaterium levansucrase (bm-ls) is hydrolysis. single domain levansucrases from gram-positive bacteria display a wide substrate-binding pocket with open access to water, challenging engineering for transfructosylation-efficient enzymes. we pursued a shift in reaction specificity by either modifying the water distribut ... | 2017 | 28575294 |
| obtaining of granular fertilizers based on ashes from combustion of waste residues and ground bones using phosphorous solubilization by bacteria bacillus megaterium. | the article presents research results on obtaining phosphorus granulated fertilizers on the basis of microbiologically activated sewage sludge ashes, ground bones and dried blood from meat industry. granulation tests were carried out using a laboratory pan granulator as well as on an experimental pilot plant. the aim of the studies was to select the proper composition of the mixture of raw materials and binding agents to obtain granulated fertilizers from waste materials such as mssa and mbm and ... | 2017 | 28501335 |
| mechanistic insights into tyrosinase-mediated crosslinking of soy glycinin derived peptides. | tyrosinase from bacillus megaterium (tyrbm) was previously used to modulate soy glycinin-based emulsions and gels. to study the crosslinking mechanism, tyrbm oxidation of three tyrosine-containing octapeptides derived from glycinin was analyzed by oxygen consumption measurements, absorbance and mass spectrometry. a significant lag period and lower activity were measured when tyrosine was located in the middle of the peptide chain. mass spectrometry analysis showed that these peptides are crossli ... | 2017 | 28490115 |
| ultraviolet/ultrasound-activated persulfate for degradation of drug by zinc selenide quantum dots: catalysis and microbiology study. | in this study, wet chemical method used for znse quantum dots (qds) and characterized by, uv-vis, photoluminescence spectroscopy, x-ray diffraction and transmission electron microscopy. the crystallites size of znse qds was 4.0nm. the average diameters of znse qds were 3.0-5.3nm. ritalin was degraded using the uv/znse qds/persulfate process. the several parameters investigated for the influence of rtialin degradation were the temperature, the persulfate concentration, and the initial ritalin con ... | 2017 | 28475974 |
| mg(2+)-induced stabilization of β-galactosidase from bacillus megaterium and its application in the galactosylation of natural products. | to improve the stability of β-galactosidase from bacillus megaterium yz08 (bmg) in aqueous hydrophilic solvents and promote its application in the galactosylation of natural products. | 2017 | 28470628 |
| new derivatives of ursolic acid through the biotransformation by bacillus megaterium cgmcc 1.1741 as inhibitors on nitric oxide production. | microbial transformation of ursolic acid (1) by bacillus megaterium cgmcc 1.1741 was investigated and yielded five metabolites identified as 3-oxo-urs-12-en-28-oic acid (2); 1β,11α-dihydroxy-3-oxo-urs-12-en-28-oic acid (3); 1β-hydroxy-3-oxo-urs-12-en-28, 13-lactoe (4); 1β,3β, 11α-trihydroxyurs-12-en-28-oic acid (5) and 1β,11α-dihydroxy-3-oxo-urs-12-en-28-o-β-d-glucopyranoside (6). metabolites 3, 4, 5 and 6 were new natural products. their nitric oxide (no) production inhibitory activity was asse ... | 2017 | 28427811 |