| responsiveness of genetically epilepsy-prone rats to intracerebroventricular morphine-induced convulsions. | the sensitivity to intracerebroventricular morphine-induced convulsions was determined in members of the severe seizure (gepr-9) and moderate seizure (gepr-3) colonies of genetically epilepsy-prone rats as well as in non-epileptic control rats. gepr-9s were more sensitive to morphine-induced wet-dog shakes, rearing with bilateral forelimb clonus and generalized clonus than controls of gepr-3s. gepr-3s were less sensitive to morphine-induced wet-dog shakes and rearing with bilateral forelimb clon ... | 1988 | 3362037 |
| effects of lesions of the dorsal noradrenergic bundle on successive discrimination in the rat. | male sprague-dawley rats were trained to bar-press for food reward on a successive discrimination involving periods of reward on a variable-interval (vi) 18-s schedule interspersed with periods of extinction. the two components of the schedule were signaled by a steady or a flashing light, counterbalanced between vi and extinction components. confirming previous findings, the discrimination was easier when the flashing light signaled vi and the steady light signaled extinction, than with the rev ... | 1988 | 3365184 |
| n-acetylcysteine inhibits human neutrophil and monocyte chemotaxis and oxidative metabolism. | the effect of n-acetylcysteine (nac) on human neutrophil and monocyte cell viability, chemotaxis, oxygen consumption and chemiluminescence was studied. it was found that nac at concentrations higher than 3 x 10(-2) m resulted in neutrophil and monocyte cytotoxicity. the studies on the effect of nac on neutrophil and monocyte chemotaxis showed that nac inhibited chemotaxis of both cell types in a concentration dependent manner. nac at 3 x 10(-2) m inhibited chemotaxis of both cell types by about ... | 1988 | 3366508 |
| a comparison of the effects of measured, predicted, estimated and constant residual volumes on the body density of female athletes. | the body density (bd), and hence the relative body fat (% bf) was measured for 182 female athletes. the residual volume (rv) was determined both before and after the underwater weighing by a multiple breath helium dilution technique with the subject immersed to neck level. the absolute mean difference (lxdl) and see between the two rv trials were 63 and 75 ml, respectively. these increased to values ranging 144-685 and 187-252 ml, respectively, when the mean of the two rv trials for each subject ... | 1988 | 3366515 |
| antimicrobial susceptibility testing of streptococcus pneumoniae: quality assessment results. | six strains of streptococcus pneumoniae were distributed to 405 united kingdom laboratories who were asked to test the susceptibility of the strains to penicillin, tetracycline, chloramphenicol and erythromycin and to provide details of methodology to test the standards of susceptibility testing. high error rates were seen only in failure to detect moderate resistance to penicillin (12%) and resistance to chloramphenicol (16%). increased error rates were associated with several methods or practi ... | 1988 | 3366921 |
| [effect of prolactin on the enzymatic activity of thyroid follicular cells during stress due to surgery and subsequent subcutaneous inflammation]. | in order to reveal prolactin (prl) effect on the enzymatic activity of the thyroid follicular cells at a subcutaneous inflammation, 2 series of experiments have been performed on 100 non-inbred male rats with body mass of 150-170 g. in order to produce the inflammation, under ether narcosis celloidin globules are introduced subcutaneously in the abdominal wall. the test animals are given 0.125 mg of "lactin" (the soviet preparation of bovine prl) in 0.5 ml of isotonic solution once every day, be ... | 1988 | 3369954 |
| [effect of caldesmon on the type of conformation changes in f-actin induced by the binding of myosin 1 subfragment]. | the effect of caldesmon on the conformational changes of f-actin caused by myosin subfragment 1 (s-1) binding was studied, using the polarized microfluorimetry method. it was demonstrated that the polarized fluorescence of rhodaminil-phalloin specifically bound to f-actin of pure actin filaments as well as of tropomyosin-containing actin filaments changes as a result of binding to s-1. the nature of these changes depends on the presence of caldesmon in the filaments. caldesmon was supposed to mo ... | 1988 | 3370245 |
| increased hepatic amino nitrogen conversion after elective cholecystectomy in man. | 1. the effect of elective, uncomplicated cholecystectomy on plasma clearances of amino acids and on amino acid-stimulated urea synthesis was investigated in 10 patients, pre-operatively and on the first post-operative day, and compared with six controls treated identically apart from the surgery. 2. a mixture of amino acids was given as a prime-continuous infusion. steady-state concentrations 75% higher than basal were attained and were maintained for 90 min. the clearances of amino acids were c ... | 1988 | 3370921 |
| characterization of size-dependent exchange of peg molecules between the blood and extravascular space in the pig. | different sized polyethylene glycols (pegs) have been used as probe molecules in studies of size-dependent permeation through the intestinal wall and the glomerular membranes. we have curve-fitted a three-compartment model to the urinary recovery data following intravenous injection of different sized peg molecules in the pig. the rate constants to and from the extravascular space demonstrate a strong size-dependent selectivity for peg molecules less than 502 da, but the rate constants to the ur ... | 1988 | 3370951 |
| trichosanthin, alpha-momorcharin and beta-momorcharin: identity of abortifacient and ribosome-inactivating proteins. | the abortifacient proteins trichosanthin, alpha-momorcharin and beta-momorcharin at nm concentrations inhibit cell-free protein synthesis. the momorcharins and the ribosome-inactivating proteins isolated from momordica charantia seeds cross-react with the respective antisera. the ribosome-inactivating proteins saporins, pokeweed antiviral protein (pap) and, to a lesser extent, gelonin have abortifacient activity on pregnant mice. | 1988 | 3372132 |
| ocular toxoplasmosis in immunosuppressed nonhuman primates. | to investigate the role of cellular immunodeficiency in recurrent toxoplasmic retinochoroiditis, six cynomolgus monkeys (macaca fascicularis) with healed toxoplasmic lesions of the retina were immunosuppressed by total lymphoid irradiation. three months prior to irradiation 30,000 toxoplasma gondii organisms of the beverley strain had been inoculated onto the macula of eye in each monkey via a pars plana approach. toxoplasmic retinochoroiditis developed in each animal, and lesions were allowed t ... | 1988 | 3372160 |
| anticonvulsant effects of dextrorphan in rats: possible involvement in dextromethorphan-induced seizure protection. | the major metabolite of the non-opioid anticonvulsant/antitussive dextromethorphan is dextrorphan. in the present study, the effects of dextrorphan were determined in an experimental model of seizure activity (maximal electroshock convulsions) (mes). subcutaneous administration of dextrorphan produced dose-related blockade of tonic hindlimb extension (the) and a decrease in the duration of tonic forelimb extension (tfe). the anticonvulsant effect of dextrorphan was linear and maximally efficacio ... | 1988 | 3374269 |
| fiberoptic angioscopy of cardiac chambers, valves, and great vessels using a guiding balloon catheter in dogs. | the applicability of fiberoptic angioscopy with a guiding balloon catheter to observe the cardiac chambers, valves and the great vessels was examined in anesthetized dogs. a no. 11 french guiding balloon catheter (balloon diameter 50 french) was introduced through either the right jugular vein or the right femoral vein into the right heart, and then a fiberscope (4.3 french in diameter) was introduced through the catheter into the right heart in 10 dogs. the balloon was inflated with air and gen ... | 1988 | 3376848 |
| [acute hepatitis: prevention and treatment]. | | 1988 | 3377335 |
| electrophysiological effects of cocaine and procaine on dorsal raphe serotonin neurons. | the effects of cocaine and procaine on the spontaneous activity of serotonin (5-hydroxytryptamine, 5-ht) neurons in the dorsal raphe nucleus of the chloral hydrate anesthetized male rat were characterized using extracellular single-unit and microiontophoretic recording techniques. intravenous cocaine elicited a potent, dose-dependent inhibition of 5-ht cell firing with a cumulative dose to 50% inhibition of cell firing of 0.66 +/- 0.11 mg/kg (means +/- s.e.m.; n = 17). in contrast, systemic admi ... | 1988 | 3384007 |
| antibody response of an elderly population to a supplemental dose of influenza b vaccine. | recent reports have suggested that the antibody response of elderly persons to standard doses of influenza vaccine is depressed. we examined the effect of an additional threefold dose of influenza b vaccine on the antibody response in elderly, ambulatory veterans. one hundred thirty-one male subjects aged 70 years and older were randomized to receive one of three influenza vaccine regimens: group i received standard trivalent influenza vaccine containing 15 micrograms of b/ussr/100/83 in one arm ... | 1988 | 3385111 |
| [study of the effect of antitumor agents on cell cycle traverse by flow cytometry]. | the effect of antitumor agents on the cell cycle traverse of cultured fl cells, a monolayer culture line derived from human amnion, was investigated by flow cytometry (fcm) in combination with simultaneous determination of total cell number, viability, mitotic index and labeling index by [3h]tdr. the results obtained can be summarized as follows: antimetabolites prolonged the duration of the s phase at a low dose, while at a high dose they decreased the rate of progression from the g1 to s phase ... | 1988 | 3389824 |
| taurine prevention of calcium paradox-related damage in cardiac muscle. its regulatory action on intracellular cation contents. | the present study was designed to investigate in chick heart whether oral pretreatment with taurine or taurine added directly to the perfusate has any effect upon calcium paradox-induced heart failure. in both protocols, taurine significantly reduced the mechanical dysfunction resulting from the calcium paradox. taurine pretreatment partially inhibited the excess accumulation of calcium in the myocardium that occurs upon calcium repletion, and microscopy revealed almost normal structure. this pr ... | 1988 | 3390225 |
| cadmium-substituted skeletal troponin c metal binding investigations and sequence assignment of the cadmium-113 resonances. | the binding of cadmium to the calcium binding subunit of skeletal troponin (stnc) has been reinvestigated using direct binding methods and fluorescent derivatives. these data provide straightforward explanations of the observed titration behavior in the 113cd nmr (ellis, p.d., strang, p., and potter, j.d. (1984) j. biol. chem. 259, 10348-10356). further, fluorescent derivatives of skeletal troponin c provide an excellent means of establishing a sequence assignment for the resonances observed in ... | 1988 | 3392014 |
| [the differentiation of bordetella bronchiseptica strains. 7. phenotypic changes in bordetella bronchiseptica through changes of phase]. | | 1988 | 3395180 |
| representation of forelimb mechanoreceptors within the superior colliculus of the cat. | forelimb mechanoreceptor representation within the superior colliculus was investigated using microelectrode recording in decerebrate or anaesthetized cats. selective activation of different mechanoreceptive fibre classes supplying the distal forelimb revealed a poor representation of all three fibre classes (sa, pc and ra) that innervate the glabrous skin. furthermore, there was no evidence for pacinian (pc) input from the interosseous or wrist region. the tap stimuli which are often needed to ... | 1988 | 3399131 |
| pharmacokinetics and pharmacodynamics of single oral doses of ibopamine, quinidine and their combination in normal man. | the pharmacokinetics and haemodynamics (phono- and impedance cardiography) of single oral doses of 200 mg ibopamine (sk&f 100168), 400 mg quinidine sulphate, and their combination, have been assessed in 6 healthy male volunteers. no significant differences in the mean pharmacokinetic parameters of either drug were seen between the single and combined doses. ibopamine caused an increase in mean estimated stroke volume (sv +29% for the maximum change from baseline and +15% cumulatively over the fi ... | 1988 | 3402527 |
| expression of fibronectin on clonally related transformed and control sublines from an epithelial cell strain and a tumor line of mouse alveolus. | the cellular expression of fibronectin and the importance of fibronectin for the morphology of cultured cells were studied in a lung epithelial cell system. the cells used were clonally related control nal1a, spontaneously transformed nal1as cells of the nal1a cell strain, and transformed clonal sublines of the cell line nal1am and the tumor cell line nul1. fibronectin was detected on the surface of nal1a cells by surface iodination; fibronectin synthesis, secretion, and pericellular accumulatio ... | 1988 | 3409226 |
| a new constrictor device for external induction of a long-term stable and irreversible renal artery stenosis in the dog. | a new externally adjustable constrictor device for renal artery stenosis in the dog is described. the constrictor combines hydraulic and mechanical characteristics, and is connected to the exterior of the animal by a thin catheter. applying hydraulic pressure via the catheter causes the plunger in the device to compress the renal artery to any desired degree of stenosis. a mechanical catch prevents backward movement of the plunger, thus ensuring a stable, irreversible renal artery constriction. ... | 1988 | 3412870 |
| production of a positive direct antiglobulin test due to suramin. | drugs and chemicals containing multiple reactive carbonyl groups have been postulated to produce nonimmunologic positive direct antiglobulin tests (dats) both in vitro and in vivo. suramin sodium, a reverse transcriptase inhibitor that was under investigation as a potential treatment for human immunodeficiency virus infection, has six reactive carbonyl groups. because of suramin's chemical structure, it was hypothesized that the drug might cause a positive dat in vitro and possibly in vivo. sura ... | 1988 | 3415441 |
| [dynamics of folliculogenesis in the mouse ovary after the intrauterine elimination of germ cells by administering cyclophosphamide]. | injection of cytostatic--cyclophosphamide--to pregnant mice produces elimination of germ cells in their offspring ovaries. independently on the elimination degree of the germ cells, the amount of growing follicles, as well as dynamics of their growth do not differ from those in the majority of control (intact) animals. in some cases the delay in the rate of the follicular growth is connected with regional outgrowth of the interstitial tissue. this, in its turn, depends on uneven distribution of ... | 1988 | 3415486 |
| metabolism of acetylene and acetaldehyde by rhodococcus rhodochrous. | we studied the ability of a soil bacterium, identified as rhodococcus rhodochrous, to grow on acetylene and to accumulate acetaldehyde. its maximum growth rate on acetylene was obtained at about 30 degrees c (mu = 0.11 h-1) and was independent of the concentration of this gas in air from 0.14 to 16% (v/v). during growth, acetylene was quantitatively transformed to acetaldehyde, ethanol, acetate, co2, and biomass in proportions which varied with culture age and temperature. growth was completely ... | 1988 | 3416237 |
| immunosuppressive substance in the sera of head and neck cancer patients. | serum levels of immunosuppressive substance (is) and immunosuppressive acidic protein (iap) were studied by single radial immunodiffusion in 108 patients with head and neck cancer. mean values of serum is and iap in patients with head and neck cancers were as follows: laryngeal carcinoma, 955 +/- 447 micrograms/ml (is), 604 +/- 324 micrograms/ml (iap); maxillary carcinoma, 896 +/- 384 micrograms/ml (is), 568 +/- 246 micrograms/ml (iap); nasopharyngeal carcinoma, 871 +/- 313 micrograms/ml (is), 6 ... | 1988 | 3416271 |
| the regional variability of enzymes in the brain: relevance to csf enzyme determinations. | the assay of cerebrospinal fluid (csf) enzymes has been suggested for assessing the extent of damage and patient prognosis in cases of brain injury. a potential difficulty associated with using csf enzyme levels as predictors of outcome is the possibility that enzyme concentrations may vary substantially from one brain region to another. we have determined the concentrations of seven enzymes in seven brain regions in the rat and cat. acid phosphatase (acp), aspartate aminotransferase (ast), isoc ... | 1988 | 3416484 |
| experimental contact sensitization with 3,4,5-trichloropyridazine. | the potential of 3,4,5-trichloropyridazine to induce contact sensitization was assessed in the guinea pig maximization test of magnusson and kligman and also in the closed patch test described by buehler. the test material was a 1% solution of 3,4,5-trichloropyridazine in a highly refined mineral oil. the test material elicited moderate to severe irritation when diluted in mineral oil to concentrations of 15-25% and minimal irritation at concentrations of 1-3%. both tests clearly indicated that ... | 1988 | 3416588 |
| effect of retinoids on the proliferation, morphology and expression of glial fibrillary acidic protein of an anaplastic astrocytoma cell line. | we studied the effect of retinoids on the growth and differentiation of a cell line (u 343 mg-a) derived from a human malignant astrocytoma. cultures treated with all-trans or 13-cis retinoic acid showed a dose-dependent inhibition of proliferation and a marked reduction in the mean cell number at the plateau phase of growth (3.5 x 10(6) vs. 1 x 10(7) cells/25 cm2) compared with untreated cultures. at confluence, cells treated with all-trans or 13-cis retinoic acid were contact-inhibited, wherea ... | 1988 | 3417370 |
| effect of gamma irradiation on the protoscoleces of echinococcus granulosus of sheep origin. | | 1988 | 3417384 |
| internal pars plana sclerotomy with the contact nd:yag laser: an experimental study. | transvitreal pars plana sclerotomy using the contact nd:yag laser with a sapphire probe was performed on a group of rabbit eyes and compared with a similar procedure performed with a ziegler knife. although the intraocular pressure (iop) was lowered in both groups of animals, the reduction was greater and more lasting in the group on which the nd:yag laser with sapphire probe was used. this technique may have application in cases with extensive peripheral anterior synechiae or where a vitrectomy ... | 1988 | 3417390 |
| optic disc, cup and neuroretinal rim size, configuration and correlations in normal eyes. | four hundred and fifty-seven unselected normal human optic nerve heads of 319 subjects (163 men, 156 women, mean age 42.7 +/- 19.6 years) were evaluated by magnification-corrected morphometry of optic disc photographs. mean optic disc surface measured 2.69 +/- 0.70 mm2 (0.80-5.54 mm2), mean diameter horizontally 1.76 +/- 0.31 mm (0.91-2.61 mm), and vertically 1.92 +/- 0.29 mm (0.96-2.91 mm). the form was slightly vertically oval. optic cup area averaged 0.72 +/- 0.70 (0.00-3.41 mm2), mean horizo ... | 1988 | 3417404 |
| hypotheses on exercise-induced pulmonary hemorrhage in horses. | | 1988 | 3417533 |
| crystallization of trimeric recombinant human tumor necrosis factor (cachectin). | crystals of tumor necrosis factor (tnf) have been obtained in two forms. rhombohedral crystals grow in 1.8 to 2.0 m ammonium sulfite, ph 7.8 at 21 degrees c, and tetragonal crystals grow in 2.6 m magnesium sulfate, ph 5.5 at 25 degrees c. analysis of tnf by isoelectric focusing under native and denaturing conditions indicates that tnf molecules exist as trimers in solution. the rhombohedral cachectin crystals belong to space group r3 and have unit cell constants a = b = c = 47.65 a and alpha = b ... | 1988 | 3417634 |
| the fate of venous repair after shock and trauma. | to assess the role of shock and trauma in the subsequent thrombosis of a venous repair, the following experiments were performed. two groups of unsplenectomized dogs were studied: control dogs (gr.c) underwent replacement of a segment of superficial femoral vein by an autogenous vein graft. trauma group (gr.t) dogs were subjected to hypovolemic shock using a modified wiggers' technique and received standardized hindleg trauma. after resuscitation, venous repairs identical to gr.c dogs were perfo ... | 1988 | 3418758 |
| pharmacokinetics of bupivacaine after short and prolonged infusions in conscious dogs. | a conscious dog model was used to study pharmacokinetics of bupivacaine after a short infusion (si) (15 min) and a prolonged infusion (pi) (24 hours). bupivacaine was infused in six mongrel dogs at least 10 days after implantation of femoral arterial and venous catheters. each dog received both the si and the pi in a random crossover design at a one week interval. bupivacaine concentration was measured in serum sampled during the si, during the last hour of the pi, and at frequent intervals duri ... | 1988 | 3421499 |
| experimental evaluation of the mitroflow pericardial heart valve prosthesis. part ii. pathologic examination. | the results of the morphologic and histopathologic examination of 37 bovine pericardial heart valve prostheses that were retrieved after experimental implantation in chacma baboons for periods of one to twelve months are presented in this study. the implanted prostheses consisted of 33 mitroflow valves, size 21 mm, that belonged to four different groups according to the method of preparation of the pericardium (process i to iv) and 4 commercially available ionescu-shiley valves, size 19 mm. all ... | 1988 | 3421507 |
| native genomic blotting: high-resolution mapping of dnase i-hypersensitive sites and protein-dna interactions. | dnase i-hypersensitive sites are observed in the promoter regions of actively expressed genes, potentially active genes, and genes that were once active. we have developed an approach that greatly increases the resolution for mapping these sites by electrophoresing genomic dna on native polyacrylamide gels prior to electroblotting and hybridization. this improved method has been used to scan the promoter and coding region of a cell-cycle-dependent human histone h4 gene with an accuracy of +/-5-1 ... | 1988 | 3422413 |
| ketone-body metabolism in hyperthyroid rats: reduced activity of d-3-hydroxybutyrate dehydrogenase in both liver and heart and of succinyl-coenzyme a: 3-oxoacid coenzyme a-transferase in heart. | the specific activity of d-3-hydroxybutyrate dehydrogenase is reduced by about a third in liver and heart mitochondria of hyperthyroid rats. state 3 respiration is also reduced in isolated mitochondria from the same animals when dl-3-hydroxybutyrate is the substrate. determination of the kinetic parameters of the membrane-bound d-3-hydroxybutyrate dehydrogenase in liver of hyperthyroid rats reveals a decreased in maximal velocity (vmax). the michaelis and dissociation constants of nad+ and d-3-h ... | 1988 | 3422549 |
| [comparison of different campylobacter-selective media in the study of feces samples of domestic animals]. | | 1987 | 3426537 |
| neutron scattering determination of the binding of prothrombin to lipid vesicles. | low-angle neutron scattering is used to study the binding of human prothrombin to small single-bilayer vesicles consisting of phosphatidylcholine and phosphatidylserine (1/1 w/w). the radius of gyration of prothrombin indicates that it is an elongated molecule. the vesicles alone were not observed to coalesce, and their molecular weight, outer radius, and average surface area per lipid were respectively (1.6 +/- 0.32) x 10(6), 114 +/- 4 a, and 110 +/- 18 a2. these values were independent of the ... | 1987 | 3427105 |
| modulation of the defective natural killer activity seen in thalassaemia major with desferrioxamine and alpha-interferon. | we previously observed that natural killer (nk) activity toward k562 cells is markedly depressed in patients with beta-thalassaemia major. here we report that these patients also exhibit significantly decreased (p less than 0.005) nk cytotoxicity against human fibroblasts infected with herpes simplex virus-type 1 (hsv-1) and that the amount of alpha-interferon (alpha-ifn) generated during the latter assays is significantly less than normal (p less than 0.005). this decreased production of alpha- ... | 1987 | 3427826 |
| delivery of theophylline into excised human skin from alkanoic acid solutions: a "push-pull" mechanism. | human skin samples are permeable to theophylline delivered from 1.5% solutions in various alkanecarboxylic acids and their mixtures. the respective permeability coefficients of theophylline, calculated from steady-state flux, correlate negatively with the permeability coefficients of the donor carboxylic acids and positively with the excess free energy of theophylline in the donor phase, or "push" effect. an exception is propionic acid which enhances the penetration of theophylline by promoting ... | 1987 | 3430340 |
| localization of source of immunosuppressive factor in the human male reproductive tract. | the source of the immunosuppressive factor (isf) in the human male reproductive tract which suppresses various in vitro immune responses has been identified. we describe here the prostatic localization of this factor, based on investigating the fractions of split ejaculates, ejaculates of individuals with blocked epididymis and the secretions of the prostate obtained by prostatic massage. | 1987 | 3430481 |
| [a new measurement of pulmonary venous compliance by r-synchronized volume loading]. | | 1987 | 3432757 |
| study of ileostomy in dogs, functional aspects, optic and scanning electronic microscopy. | containing ileostomy has been a permanent aim of researchers. different technics for containing in the ileal derivations has been proposed, very little filled the requisites, but no one reached the desired aim, and the problem continues unsolved. the authors, based in experimental study in two groups of dogs submitted to ileostomy, with and without preservation of ileal pylorus, observed the following parameters: weight dog clinical evaluation of animals (diarrhea, infection of surgical wound an ... | 1986 | 3435265 |
| heterologous protection in murine cutaneous leishmaniasis. | mice immunized with a glycolipid antigen (gl) of leishmania major plus adjuvant are relatively resistant to subsequent infection with this protozoan parasite. the gl is affinity purified on the monoclonal antibody wic-79.3 which is l. major-specific and does not react with l. donovani. when another monoclonal, wic-108.3, which cross-reacts with several leishmania species, is used to affinity purify gl from l. donovani, the eluted material can partially protect genetically resistant mice against ... | 1987 | 3436638 |
| physiological and non-physiological forms of iron affect differently proliferation and ferritin synthesis in human mononuclear cells in vitro. | the effect of iron in different forms on the proliferative response to mitogen and ferritin content of human mononuclear leukocytes (mnl) was studied in vitro. iron-loaded transferrin (fetf) but not apotransferrin (apotf) significantly enhanced the mitogenic response of mnl in serum-free culture. removal of tf from serum markedly reduced its effectiveness as a serum supplement. this effect could be almost totally reversed when the serum was reconstituted with both iron and apotf. four forms of i ... | 1987 | 3441921 |
| lipid a and related compounds. xiv. a new synthesis of lipid y, the reducing sugar moiety of salmonella- and proteus-type lipid a. | | 1987 | 3442881 |
| [pharmacological studies on y-8894. (vii). effects on transient cerebral ischemia-induced amnesia in rats]. | the effects of y-8894 on experimental amnesia in rats induced by transient cerebral ischemia (600 sec) according to the method of pulsinelli and brierley were studied using the one trial passive avoidance response and the pole climbing discrete avoidance response. all drugs were administered to the rats immediately after recirculation. the following results were obtained: 1) in the one trial passive avoidance response test, y-8894 (2.5, 5 and 10 mg/kg, i.p.) improved significantly the decreased ... | 1987 | 3443414 |
| genetic parameter estimates for preweaning growth traits in santa gertrudis cattle. | genetic parameters were estimated for birth weight and weaning weight from records collected on 1,894 santa gertrudis calves (939 bulls, 955 heifers) during the 8-yr period, 1978 through 1985. variance and covariance components were estimated separately by sex and combined across sexes utilizing mixed-model, least-squares procedures (henderson's method 3). the mathematical model assumed for estimating variance and covariance components by sex included effects of year, sire-within-year and age of ... | 1987 | 3443570 |
| serological investigations on the cause of diarrhoea on the cape verde islands. | in the serological tests 45.9% of all samples reacted positive against rota virus. 20.8% of samples revealed entamoeba histolytica positive sera, 25% shigella flexneri positive sera, 12.3% shigella sonnei and 13% shigella dysenteriae positive sera. campylobacter jejuni antibodies were found in 19% of samples, whereas 6.3% of sera reacted positive against yersinia enterocolitica type 3 and 4.8% against type 9. | 1987 | 3443746 |
| serological investigations for the cause of diarrhoea among patients and personnel in the minna and abeokuta hospitals/nigeria. | 188 blood samples were investigated for antibodies against bacteria causing diarrhoea in two hospitals in minna and abeokuta in nigeria. antibodies were found against entamoeba histolytica, yersinia enterocolitica, campylobacter jejuni, salmonella typhi, salmonella paratyphi c, shigella dysenteriae, shigella sonnei, shigella boydii, shigella flexneri and rotavirus. | 1987 | 3443752 |
| moniliella suaveolens var. nigra. | | 1987 | 3444445 |
| osteogenic sarcoma and soft tissue myxoma in a patient with fibrous dysplasia and hemoglobins jbaltimore and s. | a 41-year-old man with recognized polyostotic fibrous dysplasia since late childhood developed fibroblastic osteogenic sarcoma in the left tibia. four months after the initial diagnosis, an intramuscular myxoma was discovered in the left thigh. twenty years previously he had been found to be heterozygous for hemoglobins jbaltimore and s. malignant transformation in fibrous dysplasia is unusual and may be associated in some individuals with prior irradiation. soft tissue myxomas associated with f ... | 1986 | 3456858 |
| alpha-transforming growth factor secreted by untransformed bovine anterior pituitary cells in culture. ii. identification using a sequence-specific monoclonal antibody. | untransformed bovine anterior pituitary cells cultured in serum-free defined medium secrete an epidermal growth factor (egf)-like peptide with an amino acid composition similar to rat or human alpha-transforming growth factor (alpha tgf). to further characterize the bovine pituitary alpha tgf, it was compared to a human alpha tgf partially purified from the conditioned medium of a human melanoma cell line. an anti-alpha tgf monoclonal antibody, mf9, was produced from hybridomas derived from mice ... | 1986 | 3464598 |
| [possibility of overcoming of acnu resistance in an acnu-resistant subline of c6 rat glioma]. | membrane-modifying agents such as reserpine, calcium antagonists (nicardipine, verapamil) and calmodulin inhibitor (trifluoperazine) were found to enhance the cytotoxicity of acnu in vitro and in vivo in acnu-resistant c 6 (c 6/acnu) glioma. in in vitro experiments, uptake and retention studies with [14c] acnu revealed that intracellular uptake and retention of acnu in c 6 cells were larger than those in c 6/acnu cells, and that these membrane-modifying agents increased the cellular uptake and r ... | 1986 | 3468959 |
| neutral endopeptidase-24.11 (enkephalinase). biosynthesis and localization in human fibroblasts. | the biosynthesis, glycosylation and subcellular localization of the neutral endopeptidase-24.11 were studied in cultured human fibroblasts. the enzyme was synthesized as a precursor (mr 88,000) containing four or five n-linked oligosaccharides. within 1 h the synthesis-mature (mr 94,000) endopeptidase-24.11 was formed and contained sialylated oligosaccharides. the half-life of endopeptidase-24.11 was 3.7 days and in the presence of 10 mm-nh4cl it increased to 6 days. mature endopeptidase-24.11 w ... | 1987 | 3481263 |
| actions of prostaglandins e2 and f2 alpha on release of 14c-labelled mucins from rat submandibular salivary acini in vitro. | mucin secretion was studied in vitro using well-characterized preparations of isolated acini. pge2 significantly (p less than 0.05) increased release of [14c]-glucosamine labelled mucins at the highest concentration tested (10(-5) m), but was ineffective at lower doses (10(-9)-10(-6) m). pgf2 alpha had no effect on mucin secretion over this concentration range. pge2 (10(-9)-10(-5) m) did not modify isoproterenol stimulated mucin secretion. the cyclooxygenase inhibitor indomethacin (10(-7)-10(-5) ... | 1987 | 3482153 |
| effects of bacterial lipopolysaccharide on the binding of lymphocytes to endothelial cell monolayers. | preincubation of human umbilical vein endothelial cell (ec) monolayers with 1 ng to 10 micrograms/ml lipopolysaccharide (lps) increased the binding of t lymphocytes to ec. the effect was maximal at lps concentrations of 0.1 to 10 micrograms/ml, and occurred with lps derived from escherichia coli (serotypes 0111:b4 and 0127:b8), shigella flexneri (serotype 2a), serratia marcescens (serotype 0:3), and yersinia entercolitica (serotype 0:3). the increased binding appeared to be mediated primarily th ... | 1986 | 3484495 |
| improved survival after allogeneic small intestinal transplantation in the rat using cyclosporine immunosuppression. | we investigated the effect of cyclosporine on survival after intestinal transplantation between histoincompatible brown norway (agb3/3) and lewis (agb1/1) rats. intestinal grafts were primarily vascularized (by microsurgical techniques) and interposed isoperistaltically in the recipient's jejunum. all animals were weighed and observed daily. survival of recipients given cyclosporine 20 mg/kg/d (112 +/- 92 days, n = 10) was significantly longer (p less than .02) than that of recipients given no d ... | 1986 | 3485711 |
| characterisation of haemophilus spp. isolated from infant conjunctivitis. | haemophilus strains isolated from children under the age of 11 months with conjunctivitis were characterised by biotype, sugar fermentation, plasmid pattern and outer-membrane-protein profiles. h. influenzae was the most common species identified and was separated into 14 groups based on sugar fermentation and biotype patterns and into more than 20 groups when plasmid and outer-membrane-protein profiles were included. small (mol. wt less than 10 x 10(6)) plasmids were identified in 11 of 34 (32% ... | 1986 | 3486294 |
| possible role of protein phosphorylation in the mitogenic effect of high density lipoproteins on cultured vascular endothelial cells. | the implication of protein phosphorylation in the mitogenic action of high density lipoproteins (hdl) on bovine vascular endothelial cells was investigated by incubating endothelial cell cultures in the presence of 32p-labeled phosphoric acid. the incorporation of 32p into proteins was measured after fractionation by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate and autoradiography of the gel. in endothelial cells seeded at low density and made quiescent by serum s ... | 1986 | 3486870 |
| acrocentric/18p translocation in two mentally retarded males. delineation of the adult phenotype. | in this report we describe two adult male patients with a chromosomal rearrangement involving the short arm of chromosome 18 and an acrocentric chromosome. in addition to moderate mental retardation and verbal disability they presented dysmorphic stigmata similar to those found in the noonan syndrome. | 1986 | 3490204 |
| nucleotide sequences and properties of the sites involved in lysogenic insertion of the bacteriophage hp1c1 genome into the haemophilus influenzae chromosome. | bacteriophage hp1c1 lysogenizes its host haemophilus influenzae rd by inserting its genome into the bacterial chromosome. the dna segments corresponding to the integration regions on the phage and host chromosomes and the two junctions formed between phage and host sequences on lysogenic insertion were isolated and propagated in escherichia coli hb101 as hybrid plasmids by using pbr322 as the vector. the nucleotide sequences in the vicinity of the point of recombinational insertion were determin ... | 1987 | 3491821 |
| disk diffusion susceptibility of branhamella catarrhalis and relationship of beta-lactam zone size to beta-lactamase production. | we tested 231 isolates of branhamella catarrhalis for beta-lactamase production and drug susceptibility by the national committee for clinical laboratory standards disk diffusion method. the nitrocephin disk (cefinase) identified beta-lactamase in 98% of the enzyme-producing strains, and a zone diameter of inhibition of less than or equal to 29 mm for penicillin correctly predicted the presence of beta-lactamase in 99% of the isolates. no resistance to erythromycin, tetracycline, trimethoprim-su ... | 1986 | 3492178 |
| estrogen stimulates growth of mammary tumor cells zr-75 without activation of s6 kinase and s6 phosphorylation. difference from epidermal growth factor and alpha-transforming growth-factor-induced proliferation. | growth of the human mammary tumor cell line zr-75-1 is stimulated by epidermal growth factor (egf) and alpha-type transforming growth factor (alpha tgf), as well as by estradiol (e2). the role of activation of s6 kinase and s6 phosphorylation in the egf(alpha tgf)-induced and e2-induced growth was investigated. maximal effects on growth are observed at 10 nm egf or alpha tgf. egf as well as alpha tgf treatment of serum-starved cells leads to rapid activation of s6 kinase; the activity is increas ... | 1987 | 3494604 |
| dexamethasone pre-treatment is antipyretic toward polyinosinic: polycytidylic acid, lipopolysaccharide and interleukin 1/endogenous pyrogen. | the effect of intravenously injected dexamethasone on the febrile response of rabbits to polyinosinic: polycytidylic acid (poly i:c), lipopolysaccharide (lps) and interleukin 1/endogenous pyrogen (il1/e.p.) was studied. dexamethasone (1 mg/kg) attenuated the febrile response to poly i:c (5 micrograms/kg) but only if administered between 0.5 to 2 h before poly i:c. if it was given after poly i:c this resulted in a potentiation of the fever. antagonism of the febrile response to poly i:c by dexame ... | 1987 | 3495738 |
| local treatment with epurox (superoxide dismutase and catalase of human origin) in the chronic inflammatory and degenerative rheumatism. | | 1986 | 3496868 |
| local cerebral metabolic effects of l-dopa therapy in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine-induced parkinsonism in monkeys. | the quantitative 2-deoxy[14c]glucose autoradiographic method was used to map the distribution of alterations in local cerebral glucose utilization that accompanies clinically effective chronic l-dopa therapy of rhesus monkeys made parkinsonian by the administration of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (mptp). this pattern of changes was compared to the effects of a similar treatment regimen in normal monkeys. l-dopa (100 mg with 10 mg carbidopa) was administered orally to normal and p ... | 1987 | 3497401 |
| [experimental testing of the left-ventricular and biventricular assistant systems in dogs]. | the left-ventricular (lvad) and bi-ventricular assistant system (bvad) were tested in dogs. our hitherto existing experiences give information specially for the indication of lvad and bvad. the risk of a belated application of the lvad exists in case of only a clinical control by pressure parameters for the degree of a cardiac insufficiency caused by coronary starvations. an aortic pressure of nearly 150/70 mm hg could be reached by means bvad on condition of heart fibrillation and a left-atrial ... | 1987 | 3497493 |
| expression of the c-myc proto-oncogene in growth stimulated porcine thyroid follicle cells. | in the present investigation the expression of c-myc in porcine thyroid follicle cells following the incubation with tsh or egf was studied. egf did not only have a mitotic effect on thyroid follicles but also increased c-myc mrna. the increase in c-myc rna is an early event in egf stimulated thyroid cells. tsh had no effect on c-myc rna levels of porcine thyroid cells. egf-binding and sensitization to the mitotic activity of egf, however, is increased by tsh. while tsh stimulates camp productio ... | 1987 | 3497508 |
| difference in monoamine oxidase b activity between c57 black and albino nmri mouse strains may explain differential effects of the neurotoxin mptp. | monoamine oxidase b (mao-b) is the key enzyme in the conversion of 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine(mptp) to n-methyl-4-phenyl-pyridinium ion (mpp+) which causes degeneration of dopaminergic nigral neurons. using a histochemical tetrazolium method for mao-b with tyramine as substrate and chlorgyline for the inhibition of mao-a, black c57 mice were found to have a higher brain mao-b activity than similar aged albino nmri mice. the difference, which was in general density rather than d ... | 1987 | 3498133 |
| further evidence that there is more than one adrenal 21-hydroxylase system. | the 21-hydroxylase activity of microsomes isolated from bovine adrenal cortex have been assayed using [21-3h]17-hydroxypregnenolone and [1,2-3h]17-hydroxyprogesterone as substrates. when the assays are performed in the presence of an nadh regenerating system, to inhibit steroid 3 beta-hydroxy isomerase-dehydrogenase activity, the microsomes oxidize the 3 beta-hydroxy-5-ene steroid at a rate of 0.37 nmol/min.nmol cytochrome p-450 and the 3-keto-4-ene steroid at a rate of 6.4 nmol/min.nmol. when t ... | 1987 | 3501041 |
| the prevalence of chlamydia trachomatis in a young, sexually-active population. | a prospective study was carried out to determine the prevalence of chlamydia trachomatis among 1000 sexually-active women at the family planning association clinic in melbourne. this organism was isolated from the cervices of 5.1% of screened women. the women were surveyed about their sexual and gynaecological history, and symptoms of discharge or pain. it was found that women who gave positive results for the presence of chlamydia were younger, and had commenced intercourse at an earlier age. r ... | 1987 | 3501060 |
| detection of colonies of bacteroides gingivalis by a rapid fluorescence assay for trypsin-like activity. | | 1987 | 3507625 |
| natural cytotoxic effector cell activity against shigella flexneri-infected hela cells. | virus and facultative intracellular bacteria both replicate within a host cell. the recognition and killing of virus-infected cells by natural killer (nk) cells is thought to be an important host immune function. however, little is known about immune recognition of bacteria-infected cells. in this report, we show for the first time that human peripheral blood lymphocytes (pbl) and large granular lymphocytes (lgl) purified from pbl have significant levels of cytotoxic activity against shigella fl ... | 1986 | 3510252 |
| cutaneous shigellosis. | | 1986 | 3510587 |
| shigellaemia in adults: case reports and literature review. | | 1986 | 3510864 |
| rectal histology in acute bacillary dysentery. | a recent epidemic of acute shigella dysentery in west bengal (india) provided us with an opportunity to examine the rectal mucosal abnormalities seen in this condition. one hundred two patients were investigated using sigmoidoscopy, rectal biopsy, and rectal swab for culture. pure culture of shigella was obtained in 37 cases, and the rectal biopsy specimens from these patients were assessed in detail. the mean (+/- sd) duration of illness was 47.8 +/- 27.4 h (range 8-120 h), and most patients (3 ... | 1986 | 3510937 |
| multiplication of shigella flexneri within hela cells: lysis of the phagocytic vacuole and plasmid-mediated contact hemolysis. | virulent strains of shigella flexneri invade hela cells with high efficiency. this crucial step in the pathogenic process is encoded by a 140-megadalton plasmid which induces phagocytosis of the bacteria by host cells. in this report we used pwr100, the virulence plasmid of s. flexneri serotype 5, and phs4108, a 32-megadalton subclone of pwr100, to demonstrate that the plasmid is also responsible for rapid intracellular growth of the bacteria. the ability to replicate intracellularly was not cor ... | 1986 | 3510976 |
| molecular cloning of a genetic determinant for congo red binding ability which is essential for the virulence of shigella flexneri. | a dna sequence of about 1.0 kilobase (kb) derived from a 230-kb (140-megadalton) plasmid in a fully virulent shigella flexneri 2a strain, ysh6000, was cloned into escherichia coli k-12 by selecting for the ability to bind congo red (pcr+ phenotype). it was mapped and localized within the sali restriction fragment f on the plasmid. this clone converted an s. flexneri strain which is avirulent as a result of a small deletion in the plasmid to full virulence but did not convert those without the 23 ... | 1986 | 3510977 |
| expression of several adhesive macromolecules (n-cam, l1, j1, nile, uvomorulin, laminin, fibronectin, and a heparan sulfate proteoglycan) in embryonic, adult, and denervated adult skeletal muscle. | levels of the neural cell adhesion molecule n-cam in muscle are regulated in parallel with the susceptibility of muscle to innervation: n-cam is abundant on the surface of early embryonic myotubes, declines in level as development proceeds, reappears when adult muscles are denervated or paralyzed, and is lost after reinnervation (covault, j., and j. r. sanes, 1985, proc. natl. acad. sci. usa, 82:4544-4548). here we used immunocytochemical methods to compare this pattern of expression with those ... | 1986 | 3511069 |
| 1-nitropyrene: a mutagenic product induced by the action of near ultraviolet light on 1-aminopyrene. | a solution of 1-aminopyrene in dimethyl sulfoxide exposed to an artificial source of near ultraviolet light (600 kj/m2) induced significant direct-acting mutagenicity in the ames/salmonella plating assay utilizing strain ta98. high-performance liquid chromatography of this solution resulted in a fraction that was mutagenic on ta98 but inactive on a nitroreductase-deficient strain of salmonella (ta98nr). this observation suggested the presence of a nitro-containing compound. mass spectral analysi ... | 1986 | 3511370 |
| adherence of non-fimbriate entero-invasive escherichia coli o124 to guinea pig intestinal tract in vitro and in vivo. | the adherence properties of non-fimbriate entero-invasive escherichia coli o124 in the guinea pig intestinal tract were studied. quantitative in-vitro determinations were done by incubating radiolabelled bacteria with suspensions of viable intestinal cells released by treating loops of the guinea pig intestine with solutions containing edta, dithiothreitol and citrate. non-bound bacteria were separated from the intestinal cells on a percoll gradient. only cells released from the colon, especiall ... | 1986 | 3512837 |
| activation of the complement system by cryptococcus neoformans leads to binding of ic3b to the yeast. | the complement system plays a key role in resistance to cryptococcosis. in the present study, we examined several factors that influence the binding of c3 cleavage fragments to cryptococcus neoformans. binding of c3 was determined by using normal human serum supplemented with 125i-labeled c3. incubation of encapsulated cryptococci in 20% serum led to the binding of approximately 3.2 x 10(6) molecules of c3 to each cell. the binding of c3 was markedly inhibited by heating the serum at 56 degrees ... | 1986 | 3514450 |
| degradation of insulin by insulin-degrading enzyme and biological characteristics of its fragments. | we have previously reported on the purification, characterization, and biological significance of insulin-degrading enzyme (ide) from pig and rat skeletal muscle. in the present study, we have investigated the detection and the hplc separation of degradation products of native insulin from the reaction of monocomponent porcine insulin with affinity-purified pig ide. insulin was degraded by ide in a time- and dose-dependent manner. eight peaks (peaks i through viii) appeared after 1 h of incubati ... | 1986 | 3516656 |
| coordinate roles of insulin and glucose on the growth of hepatoma cells in culture. | | 1986 | 3516664 |
| the role of shigella spp., enteroinvasive escherichia coli, and other enteropathogens as causes of childhood dysentery in thailand. | shigella spp. were isolated from 44%, pleisomonas shigelloides from 22%, campylobacter spp. from 16%, and salmonella spp. from 10% of 200 thai children with mucoid or bloody diarrhea. enteroinvasive escherichia coli (eiec) was identified by examining isolates of e. coli for plasmids larger than 120 megadaltons (mda), by identifying e. coli with a virulence marker antigen in an elisa, and by performing dna hybridization with a 17-kilobase (kb) ecori digestion fragment of plasmid pwr100 (a 140-mda ... | 1986 | 3517189 |
| shigella bacteremia in adults. | | 1986 | 3517402 |
| characterization of transferable plasmids from shigella flexneri 2a that confer resistance to trimethoprim, streptomycin, and sulfonamides. | a set of plasmids conferring resistance to several antibiotics, including the combination of trimethoprim and sulfamethoxazole, has been isolated from escherichia coli following conjugative cotransfer from a clinical isolate of shigella flexneri 2a. one of the plasmids, pcn1, was shown by subcloning and dna sequencing to carry a gene encoding a trimethoprim-insensitive dihydrofolate reductase identical to that found in e. coli transposon 7. this plasmid was also shown to confer resistance to bot ... | 1986 | 3517903 |
| [population migrations and the epidemic process of flexner and sonne dysentery]. | analysis of the materials from the khabarovsk territory for the representative period of 25 years (1956-1980) revealed the influence of the migration of the population on the level and dynamics of morbidity in different kinds of dysentery, flexner's dysentery shown to occur more frequently than sonne dysentery. intensive migrations increase the proportion of susceptible persons among the population, thus facilitating the formation and circulation of shigella strains with pronounced virulence. | 1986 | 3518299 |
| [development of the classification of bacteria in the genus shigella]. | | 1986 | 3518304 |
| [bactericidal and immunocorrective properties of plant extracts]. | extract from oak cork, st. john's wort leaves and flowers and pine buds possess more pronounced bactericidal properties with respect to staphylococci, shigellae, escherichia coli than decoctions from these medicinal plants. such extracts may be included into complexes for the treatment of otorhinolaryngological diseases, enterocolitis in children and bacterial eczema. as a medicinal form, extract prepared from medicinal plants are more convenient for storage and permit more exact dosage. | 1986 | 3518308 |
| comparative efficacies of single intravenous doses of ceftriaxone and ampicillin for shigellosis in a placebo-controlled trial. | to evaluate ceftriaxone for the treatment of shigellosis, 94 adult males with acute dysentery were randomly assigned to receive ceftriaxone (1 g), ampicillin (4 g), or saline placebo intravenously in single doses in a double-blind design. stool cultures were positive for shigella dysenteriae in 52 patients, s. flexneri in 38 patients, and other species in 4 patients. both ceftriaxone and ampicillin caused reductions in the mean duration of fever and the means of daily stool frequency 2 to 4 days ... | 1986 | 3518625 |
| metabolic rhythms in adolescents with diabetes during treatment with porcine or human insulin. | metabolic rhythms were studied over 24 hours in eight adolescent diabetic patients during treatment with porcine insulin and after transfer to human insulin. despite an increase in dose with human insulin no significant changes were found in fasting blood glucose, 24h mean blood glucose, or glycosylated haemoglobin concentrations. significantly higher 24h mean blood lactate concentrations and lower total ketone bodies and glycerol concentrations were observed during treatment with human insulin. ... | 1986 | 3518640 |
| identification and partial characterization of a nucleolar antigen with a molecular weight of 145,000 found in a broad range of human cancers. | previous studies in our laboratory have indicated the presence of nucleolar antigens in tumors which were not detected in normal tissues. some of the polyclonal antisera produced in these studies were shown to identify a mr, 145,000 nucleolar antigen on immunoblots of tumor nucleoli but not in normal human liver nucleoli. a monoclonal antibody to a mr 145,000 nucleolar protein (p145) was produced by immunization of mice with a nucleolar extract of hela cells which is enriched with this antigen. ... | 1986 | 3518918 |
| immunoglobulin g (igg) and iga subclass pattern of human antibodies to shigella flexneri and salmonella serogroup b and d lipopolysaccharide o antigens. | the subclass distribution of human serum antibodies to the o-antigenic lipopolysaccharides of salmonella serogroups b and d and to shigella flexneri serotypes 1b, 2a, and 4a lipopolysaccharide antigens were analyzed in an enzyme-linked immunosorbent assay with monoclonal antibodies to the immunoglobulin subclasses. the patients had culture-verified salmonella (17 swedes) or shigella flexneri (23 vietnamese; 11 children and 12 adults) infections. consecutive samples drawn during 1 year postinfect ... | 1986 | 3519461 |