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identification of new bacteria harboring qnrs and aac(6')-ib/cr and mutations possibly involved in fluoroquinolone resistance in raw sewage and activated sludge samples from a full-scale wwtp.wastewater treatment plants (wwtps) harbor bacteria and antimicrobial resistance genes, favoring gene exchange events and resistance dissemination. here, a culture-based and metagenomic survey of qnra, qnrb, qnrs, and aac(6')-ib genes from raw sewage (rs) and activated sludge (as) of a full-scale municipal wwtp was performed. a total of 96 bacterial isolates were recovered from nalidixic acid-enrichment cultures. bacteria harboring the aac(6')-ib gene predominated in rs, whereas qnrs-positive is ...201727984803
protein content analysis and antimicrobial activity of the crude venom of montivipera bornmuelleri; a viper from lebanon.viperidae snakes venoms represent a source of efficient bioactive components that have already led to the development of several new drugs. in this work, we analyzed the protein content of the montivipera bornmuelleri crude venom using lc-esi-ms, sephadex g-75 gel filtration and sds-page and demonstrated the presence of proteins with molecular masses corresponding to metalloprotease iii, serine-protease and pla2 in three fractions collected after gel filtration. equally, we examined the antimicr ...201424853875
antimicrobial activity and chemical composition of the essential oils of portuguese foeniculum vulgare fruits.the aim of this study was to investigate the chemical composition and antimicrobial activity of essential oils obtained by hydrodistillation from fruits of six fennel accessions collected from wild populations occurring in the centre and south of portugal. composition of essential oils was established by gas chromatography-flame ionization detector (gc-fid) and gas chromatography-mass spectrometry (gc-ms) analysis. the obtained yields of the essential oils were found to vary greatly in the range ...201525973507
antimicrobial activity of schinus lentiscifolius (anacardiaceae).schinus lentiscifolius marchand (syn. schinus weinmannifolius engl) is a plant native to rio grande do sul (southern brazil) and has been used in brazilian traditional medicine as antiseptic and antimicrobial for the treatment of many different health problems as well as to treat leucorrhea and to assist in ulcer and wound healing. although it is a plant widely used by the population, there are no studies proving this popular use.201323684720
purification, characterization and functional analysis of the immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus and their antibiofilm properties.the present study reveals purification and characterization of immune molecule lectin from the haemolymph of blue swimmer crab portunus pelagicus (pp-lec). the pp-lec was purified by affinity chromatography with mannose coupled sepharose cl-4b column and it exhibits single band with a molecular weight of 155 kda in sds-page. the surface morphology of purified pp-lec displays the homogeneous nature of protein. a distinct peak with a retention time of 3.3 min was appeared in high performance liqui ...201728110033
biofilm colonization in chronic treatment refractory infections presenting with discharging sinuses: a study in a tertiary care hospital of eastern india.treatment refractory chronic recurrent infections mean those chronic infections which recur by same causal agents with similar drug responsiveness after apparent relief following full course of recommended antimicrobial management.201728367029
can surgical need in patients with naja atra (taiwan or chinese cobra) envenomation be predicted in the emergency department?to investigate the clinical predictors and the aetiologies for surgery in patients with naja atra (taiwan or chinese cobra) envenomation.201627516567
proteolytic activity by multiple bacterial species isolated from chronic venous leg ulcers degrades matrix substrates.a major feature of chronic wounds is the loss of tissue, with the exposure of dermal components preventing primary closure and leading to bacterial colonization. bacterial colonization has been proposed as one of the common underlying pathologies present in chronic wounds. the objective of this exploratory study was to identify bacteria cultured from chronic venous leg ulcers and test for proteolytic activity that degrades matrix substrates.201323118301
infectious bacterial pathogens, parasites and pathological correlations of sewage pollution as an important threat to farmed fishes in egypt.this paper is a part of a multi-disciplinary research "application of decentralized on-site water treatment system in egypt for use in agriculture and producing safe fish and animal proteins". the project aimed to investigate the environmental impact of implementing sewage water before and after treatment using the effluent of the on-site decentralized japanese' johkasou system, in agriculture and producing fish protein. the aim is to establish such system in egypt to strengthen the sanitary con ...201627720545
[effect of colistin consumption and prevalence of colistin-resistant bacteria].recently, there has been a renaissance of the use of the antibiotic colistin resulting from increasing resistance of bacterial pathogens, particularly in intensive care patients. the study aimed at assessing the impact of colistin consumption on the prevalence of colistin-resistant bacteria in the university hospital olomouc (uho).201323965814
impact of revised broad-spectrum cephalosporin clinical and laboratory standards institute breakpoints on susceptibility in enterobacteriaceae producing ampc β-lactamase.we evaluated the impact of revised clinical and laboratory standards institute (clsi) breakpoints for broad-spectrum cephalosporins (bscs) on the susceptibilities of 1,742 isolates of enterobacter species, serratia marcescens, citrobacter freundii, and morganella morganii. the 2011 clsi criteria for cefotaxime and ceftazidime reduced the rates of susceptibility by 2.9% and 5.9%, respectively. the 2014 clsi criteria for cefepime reduced the rate of susceptibility by 13.9%, and categorized 11.8% i ...201728271652
[antimicrobial resistance monitoring of gram-negative bacilli isolated from 15 teaching hospitals in 2014 in china].to investigate the current situation of antimicrobial resistance of nosocomial gram-negative bacilli in 2014 in china.201526675021
intestinal enterobacteriaceae and escherichia coli populations in japanese adults demonstrated by the reverse transcription-quantitative pcr and the clone library analyses.a primer set specific for escherichia coli/shigella 16s rrna was developed and used for rt-qpcr analysis of fecal samples from 35 healthy adult volunteers in combination with the previously reported primer set specific for enterobacteriaceae. enterobacteriaceae and e. coli were present in the 29 out of 35 volunteers tested as intestinal commensals at the average population levels of 10(7.1±0.9) and 10(6.8±0.7)cellsg(-1) of stools (mean±standard deviation), respectively. among the 7 volunteers, t ...201323262033
characterization of aminoglycoside-modifying enzymes in enterobacteriaceae clinical strains and characterization of the plasmids implicated in their diffusion.a total of 788 clinical enterobacteriaceae were collected to describe the aminoglycoside-modifying genes (ame genes) and to characterize the plasmids that carry these genes. among the 788 strains collected, 330 (41.8%) were aminoglycoside-resistant: 264 escherichia coli (80%), 33 proteus mirabilis (10%), 10 klebsiella pneumoniae (3%), six k. oxytoca (1.8%), five enterobacter cloacae (1.5%), three morganella morganii (0.9%), three providencia stuartii (0.9%), two salmonella enterica (0.6%), and o ...201323206280
antibiotic therapy for inducible ampc β-lactamase-producing gram-negative bacilli: what are the alternatives to carbapenems, quinolones and aminoglycosides?some bacteria that possess chromosomally determined ampc β-lactamases may express these enzymes at a high level following exposure to β-lactams, either by induction or selection for derepressed mutants. this may lead to clinical failure even if an isolate initially tests susceptible in vitro, a phenomenon best characterised by third-generation cephalosporin therapy for enterobacter bacteraemia or meningitis. several other enterobacteriaceae, such as serratia marcescens, citrobacter freundii, pro ...201222824371
in vitro activity of tigemonam against urinary tract pathogens.tigemonam's in vitro activity was compared with that of aztreonam, cefotaxime, gentamicin, and norfloxacin. thirty-two strains of pseudomonas and 960 strains of enterobacteriaceae were studied using the agar dilution method (including escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, enterobacter sp., serrada marcescens, proteus mirabilis, proteus vulgaris, morganella morganii, and salmonella enterica). tigemonam displayed good activity against enterobacteriaceae. minimum inhibitory c ...198927416146
low-level antimicrobial resistance of enterobacteriaceae isolated from the nares of pig-exposed persons.extended-spectrum β-lactamase (esbl)-producing enterobacteriaceae (esbl-e) have recently emerged in livestock and humans. therefore, this study assessed the carriage of enterobacteriaceae in the anterior nares and associated antimicrobial resistance in pig-exposed persons. nasal swabs were enriched in non-selective broth and then plated on macconkey and esbl-selective agars. species was confirmed by matrix-assisted laser-desorption ionization-time-of-flight mass spectrometry (maldi-tof ms). anti ...201626219671
increased iga and igm responses against gut commensals in chronic depression: further evidence for increased bacterial translocation or leaky gut.recently, we discovered that depression is accompanied by increased igm and iga responses directed against gram negative gut commensals. the aim of this study was to replicate these findings in a larger study group of depressed patients and to examine the associations between the iga and igm responses to gut commensals and staging of depression as well as the fatigue and somatic (f&s) symptoms of depression.201222410503
very high prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bacteriemic patients hospitalized in teaching hospitals in bamako, mali.the worldwide dissemination of extended-spectrum beta-lactamase producing enterobacteriaceae, (esbl-e) and their subset producing carbapenemases (cpe), is alarming. limited data on the prevalence of such strains in infections from patients from sub-saharan africa are currently available. we determined, here, the prevalence of esbl-e/cpe in bacteriemic patients in two teaching hospitals from bamako (mali), which are at the top of the health care pyramid in the country. during one year, all entero ...201728245252
sonication of abdominal drains: clinical implications of quantitative cultures for the diagnosis of surgical site infection.the use of drains in patients undergoing abdominal surgery has been a subject of debate for several decades. in this paper, the usefulness of quantitative cultures of sonicated abdominal drains for diagnosing surgical site of infection (ssi) and the association between culture results with patient outcome is evaluated.201627046739
emergence of new sequence type oxa-48 carbapenemase-producing enterobacteriaceae in kuwait.the aim of this study was to investigate the infections due to oxa-48 carbapenemase-producing bacteria in tertiary hospitals in kuwait (september 2011 to april 2013) and to determine the sequence types (sts) of the corresponding isolates. eleven oxa-48 carbapenemase-producing enterobacteriaceae isolates were recovered from patients treated in nine different hospitals in kuwait. susceptibility testing to eighteen antibiotics was done using the e-test. pcr assays were performed for the detection o ...201525551428
countrywide spread of oxa-48 carbapenemase in lebanon: surveillance and genetic characterization of carbapenem-non-susceptible enterobacteriaceae in 10 hospitals over a one-year period.to detect, characterize, and assess the genetic clonality of carbapenem-non-susceptible enterobacteriaceae in 10 lebanese hospitals in 2012.201425449248
emergence of ndm-1-producing enterobacteriaceae in porto alegre, brazil.to evaluate the emergence of new delhi metallo-β-lactamase 1 (ndm-1)-producing enterobacteriaceae isolates in brazil.201424857802
activity of fosfomycin and comparison of several susceptibility testing methods against contemporary urine isolates.fosfomycin is recommended as first-line treatment for acute uncomplicated cystitis in women. it has demonstrated in vitro activity against a variety of pathogens; however, a paucity of data are available from the usa. we determined the susceptibility of a collection of urine isolates to fosfomycin and compared multiple methods of susceptibility testing. consecutive non-duplicate enterobacteriaceae, enterococci and pseudomonas aeruginosa isolates were collected from the clinical microbiology labo ...201526498988
susceptibility patterns of uropathogens identified in hospitalised children with community-acquired urinary tract infections in thrace, greece.urinary tract infections (utis) are common infections associated with considerable morbidity and mortality, particularly in paediatric patients. the alarmingly increasing antimicrobial resistance of contemporary uropathogens in children necessitates the re-evaluation of antibiotic treatment. we evaluated uropathogens isolated from children hospitalised due to a community-acquired uti over a 5.5-year period in a university hospital of northern greece and their antibiotic susceptibility patterns. ...201527873675
role of the bacterial vaccine solco-urovac® in treatment and prevention of recurrent urinary tract infections of bacterial origin.urinary tract infections (uti) represent a serious medical problem with considerably high rate of morbidity. recurrent episodes of urinary tract infection (ruti) may commonly develop in the way of relapse or reinfection. for patients, ruti is always associated with costs, discomfort and decreased quality of life. standard treatment of ruti is through antibiotics and usually such treatment is required repeatedly. repeat course of antibiotics leads to increase of resistance of uropathogenic strain ...201425020163
bacteria isolated from dugongs (dugong dugon) submitted for postmortem examination in queensland, australia, 2000-2011.microbial infection may contribute to disease in a significant proportion of marine mammal mortalities, but little is known about infectious bacterial species and their prevalence in dugongs (dugong dugon). this study represents a survey of the species of bacteria and fungi isolated from dugongs submitted to the university of queensland's school of veterinary science for postmortem examination. thirty-six dugongs were included in the survey, with 23 species of bacteria and four species of fungus ...201323505701
characterization of complex, heterogeneous lipid a samples using hplc-ms/ms technique ii. structural elucidation of non-phosphorylated lipid a by negative-ion mode tandem mass spectrometry.non-phosphorylated lipid a species confer reduced inflammatory potential for the bacteria. knowledge on their chemical structure and presence in bacterial pathogens may contribute to the understanding of bacterial resistance and activation of the host innate immune system. in this study, we report the fragmentation pathways of negatively charged, non-phosphorylated lipid a species under low-energy collision-induced dissociation conditions of an electrospray ionization quadrupole time-of-flight i ...201628239963
high carbapenem resistance in clinical gram-negative pathogens isolated in egypt.the emergence and spread of carbapenem-resistant gram-negative bacteria poses a serious threat to human health worldwide. currently, little is known about the molecular mechanisms underlying carbapenem resistance and their prevalence among gram-negative bacteria in egypt. in this study, we analyzed carbapenemase production in gram-negative bacteria isolated from hospitalized patients in egypt in 2014. all isolates were subjected to phenotypic and genotypic susceptibility testing for carbapenem r ...201728191865
extended-spectrum beta-lactamase- and carbapenemase-producing enterobacteriaceae among ethiopian children.infections by extended-spectrum beta-lactamase- (esbl) and carbapenem-resistant enterobacteriaceae (cre) are an emerging problem in children nowadays. hence, the aim of this study was to determine the prevalence of esbl- and carbapenemase-producing enterobacteriaceae among children suspected of septicemia and urinary tract infections (utis).201728182124
antioxidant, anticholinesterase and antibacterial activities of stachys guyoniana and mentha aquatica.stachys guyoniana noë ex. batt. and mentha aquatica l. are two algerian lamiaceae used in folk medicine.201727927090
draft genomic analysis of an avian multidrug resistant morganella morganii isolate carrying qnrd1.morganella morganii is a commensal bacterium and opportunistic pathogen often present in the gut of humans and animals. we report the 4.3 mbp draft genome sequence of a m. morganii isolated in association with an escherichia coli from broilers in portugal that showed macroscopic lesions consistent with colisepticemia. the analysis of the genome matched the multidrug resistance phenotype and enabled the identification of several clinically important and potentially mobile acquired antibiotic resi ...201627826290
characterization of complex, heterogeneous lipid a samples using hplc-ms/ms technique i. overall analysis with respect to acylation, phosphorylation and isobaric distribution.we established a new reversed phase-high performance liquid chromatography method combined with electrospray ionization quadrupole time-of-flight tandem mass spectrometry for the simultaneous determination and structural characterization of different lipid a types in bacteria (escherichia coli o111, salmonella adelaide o35 and proteus morganii o34) showing serological cross-reactivity. the complex lipid a mixtures (obtained by simple extraction and acid hydrolysis of the outer membrane lipopolys ...201627506631
multi-typing of enterobacteria harboring lt and st enterotoxin genes isolated from mexican children.enterotoxigenic escherichia coli is the most common cause of diarrhea in children younger than 5 years in the developing world. we used 16s rrna gene sequencing, the biolog(®) system, and an amplified ribosomal dna restriction analysis (ardra) to identify 69 enterobacteria isolated from the feces of healthy children up to 12 years old and 54 enterobacteria isolated from stool samples obtained from children up to 5 years old with diarrhea from morelia, michoacán, mexico. in the diarrheic group, 1 ...201727169941
infected cephalohematomas and underlying osteomyelitis: a case-based review.cephalohematomas are relatively common sub-periosteal collections of blood that tend to resolve spontaneously without treatment. rarely, they become infected and can be associated with underlying osteomyelitis, meningitis, or sepsis. common pathogens include escherichia coli and staphylococcus species.201627066799
[morpho-functional characteristic of enterobacteria adhesive activity].study adhesive activity of enterobacteria in the model of hemagglutination reaction with animal and avian erythrocytes and clarify structures responsible for adhesion in enterobacteria.201626829846
follow-up after infants younger than 2 months of age with urinary tract infection in southern israel: epidemiologic, microbiologic and disease recurrence characteristics.the timing of most recurrences after neonatal urinary tract infection is during the first year of life, with peak incidence 2-6 months after the initial infection. information on the microbiologic characteristics of recurrent urinary tract infection episodes in relation to the microbiology of the initial episodes is limited.201626607682
enterobacter strains might promote colon cancer.many studies have been performed to determine the interaction between bacterial species and cancer. however, there has been no attempts to demonstrate a possible relationship between enterobacter spp. and colon cancer so far. therefore, in the present study, it is aimed to investigate the effects of enterobacter strains on colon cancer. bacterial proteins were isolated from 11 enterobacter spp., one morganella morganii, and one escherichia coli strains, and applied onto ncm460 (incell) and crl17 ...201526143056
molecular docking studies of the antitumoral activity and characterization of new chalcone.phytochemical investigation of azorella madreporica led to the isolation of four known compounds and an unknown chalcone. the structure of the new compound was identified by spectroscopy, including two-dimensional nmr techniques and comparison with published spectral data. the antioxidant activity of chalcone (compound 1) was measured using the 1,2-diphenyl-2-picryl-hydrazyl (dpph) free radical scavenging assay, and the bioactivity was evaluated against five bacteria (mycobacterium smegmatis atc ...201525915607
structural background for serological cross-reactivity between bacteria of different enterobacterial serotypes.the structure of the oligosaccharide repeating units of endotoxins from gram-negative bacteria is characteristic for the different serogroups and serotypes of bacteria. detailed examination of the cross-reactions of three enterobacterial serotypes, proteus morganii o34, escherichia coli o111, and salmonella enterica sv. adelaide o35, was performed using sensitive tests (elisa, immunoblotting). fine differences between the endotoxins of the bacteria were detected using silver staining of sds-page ...201525630395
sequencing, characterization, and gene expression analysis of the histidine decarboxylase gene cluster of morganella morganii.the histidine decarboxylase gene cluster of morganella morganii dsm30146(t) was sequenced, and four open reading frames, named hdct1, hdc, hdct2, and hisrs were identified. two putative histidine/histamine antiporters (hdct1 and hdct2) were located upstream and downstream the hdc gene, codifying a pyridoxal-p dependent histidine decarboxylase, and followed by hisrs gene encoding a histidyl-trna synthetase. this organization was comparable with the gene cluster of other known gram negative bacter ...201424241330
adverse effects of members of the enterobacteriaceae family on boar sperm quality.semen samples collected in 2012 from 1785 boars belonging to five different breeds were recruited from the quality control laboratory of magapor sl, spain. these samples came from 43 boar studs and resulted from diluting the ejaculates in commercial semen extenders. evaluation of the semen sample characteristics (color, smell, ph, osmolality, concentration, motility of sperm cells, agglutination, acrosome integrity, short hypoosmotic swelling test, and abnormal forms) revealed that they met the ...201323827823
commensal enterobacteriaceae as reservoirs of extended-spectrum beta-lactamases, integrons, and sul genes in portugal.bacteria colonizing the human intestine have a relevant role in the spread of antimicrobial resistance. we investigated the faecal carriage of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in healthy humans from portugal and analyzed the distribution of sul genes and class 1 and 2 integrons. faecal samples (n = 113) were recovered from healthy persons (north/centre of portugal, 2001-2004) and plated on macconkey agar with and without ceftazidime (1 mg/l) or cefotaxime (1 m ...201323579192
antibiotic selection for the treatment of infectious complications of implant-based breast reconstruction.infection requiring explantation remains the most devastating complication associated with implant-based breast reconstruction. there are many treatment algorithms to prevent reconstructive failure in face of infection using both oral and intravenous antibiotics. in the absence of patient-specific culture data, antibiotic selection is generally directed toward broad-spectrum coverage based on historical data. we hypothesize that reviewing our institution's microbiology data obtained from explant ...201323486147
[purple urine bag syndrome in two institutionalised patients].purple urine bag syndrome (pubs) is an uncommon but particularly striking phenomenon characterised by a chemical reaction involving the urine, plastic and certain enzymes from some sulphatase- and phosphatase-producing bacteria, including proteus mirabilis, escherichia coli and morganella morganii, amongst others. following this reaction, the catheter and the bag may be stained red, blue or purple. this phenomenon tends to occur in patients with multiple pathology and with urinary catheters, as ...201323199817
porin alterations present in non-carbapenemase-producing enterobacteriaceae with high and intermediate levels of carbapenem resistance in chile.the main goal of this work was to identify the mechanisms responsible for carbapenem resistance in 61 chilean clinical isolates of enterobacteriaceae (enterobacter spp., serratia marcescens, morganella morganii, escherichia coli and klebsiella pneumoniae) with reduced susceptibility to at least one carbapenem (ertapenem, imipenem or meropenem). all of the isolates were analysed for the presence of carbapenemases, extended spectrum β-lactamases (esbls), ampc enzymes and outer-membrane proteins. n ...201222700549
detection of kpc-2 and qnrs1 in clinical isolates of morganella morganii from china.seven carbapenem-nonsusceptible morganella morganii isolates, which have similar antibiotic susceptibility profiles, were isolated over a 5-month period. mics of imipenem, meropenem, and ertapenem were 8, 1, and 0.25 to 0.5 μg/ml, respectively. pulsed-field gel electrophoresis indicated that 6 isolates were indistinguishable or closely related. carbapenem resistance can be transferred from m. morganii to escherichia coli by conjugation. all m. morganii isolates and e. coli transconjugants produc ...201222542894
heat resistance of histamine-producing bacteria in irradiated tuna loins.consumption of foods high in biogenic amines leads to an illness known as histamine, or scombrotoxin, poisoning. the illness is commonly associated with consumption of fish with high levels of histamine ( $ 500 ppm). the objective of this study was to determine and compare the heat resistance of five histamine-producing bacteria in irradiated albacore tuna loins. heat-resistance parameters (d- and z-values) were determined for morganella morganii, raoultella planticola, hafnia alvei, and enterob ...201323992506
bacterial microbiota of the ocular surface of captive and free-ranging microbats: desmodus rotundus, diameus youngi and artibeus lituratus.to investigate normal aerobic bacterial microbiota of the eye surface in vvcaptive and free-ranging bats belonging to the suborder microchiroptera.201423710696
in vivo acquisition of carbapenemase gene blakpc-2 in multiple species of enterobacteriaceae through horizontal transfer of insertion sequence or plasmid.objectives: current worldwide spread of carbapenem resistance in enterobacteriaceae constitutes a critical public health threat. this study aims to investigate how carbapenem resistance is acquired in enterobacteriaceae in patients during antimicrobial therapy. methods: clinical strains from the same anatomical site of the same patients that converted from carbapenem-susceptible to resistant during antimicrobial therapy and showed identical or similar pfge patterns were identified. the similarly ...201627818649
prevalence and characterization of high histamine-producing bacteria in gulf of mexico fish species.recent developments in detection and enumeration of histamine-producing bacteria (hpb) have created powerful molecular-based tools to better understand the presence of spoilage bacteria and conditions, resulting in increased risk of scombrotoxin fish poisoning. we examined 235 scombrotoxin-forming fish from the gulf of mexico for the presence of high hpb. photobacterium damselae subsp. damselae was the most prevalent hpb (49%), followed by morganella morganii (14%), enterobacter aerogenes (4%), ...201526197285
determination and frying loss of histamine in striped marlin fillets implicated in a foodborne poisoning.an incident of foodborne poisoning causing illness in 67 victims due to ingestion of fried fish fillets occurred in june 2011, in southern taiwan. of the five suspected fish fillets, one fried sample contained 62.0 mg/100 g and one raw sample contained 89.6 mg/100 g histamine, levels which are greater than the potential hazard action level (50 mg/100 g) in most illness cases. given the allergy-like symptoms of the victims and the high histamine content in the suspected fish samples, this foodbor ...201323643129
serratia marcescens, morganella morganii, klebsiella oxytoca related peritonitis attacks in a patient on automated peritoneal dialysis: a case report. 201728256286
evaluation of drug-resistant enterobacteriaceae in retail poultry and beef.there has been increasing concern on the emergence of multidrug-resistant foodborne pathogens from foods of animal origin, including poultry. the current study aimed to evaluate antibiotic-resistant enterobacteriaceae from raw retail chicken/turkey parts (thigh, wings, breast, and ground) and beef meat (ground and chunks) in middle tennessee. resistance of the collected enterobacteriaceae to a panel of antibiotics was determined by the kirby-bauer disk diffusion test. retail meats were also assa ...201323472034
dha-1 plasmid-mediated ampc β-lactamase expression and regulation of klebsiella pnuemoniae isolates.the present study aimed to investigate the regulatory mechanism of the ampc enzyme by analyzing the construction and function of ampcr, ampe and ampg genes in the dhahran (dha)‑1 plasmid of klebsiella pneumoniae (k. pneumoniae). the production of ampc and extended‑spectrum β‑lactamase (esbl) were determined following the cefoxitin (fox) inducing test for ampc, preliminary screening and confirmation tests for esbl in 10 dha‑1 plasmid ampc enzymes of k. pneumoniae strains. ampcr, ampd, ampe and am ...201525483576
comparison of in vitro efficacy of ertapenem, imipenem and meropenem in the infections caused by the enterobacteriaceae strains family.the members of the bacterial enterobacteriaceae family play an important role in the aetiology of many hospital infections. some of them are able to produce β-lactamase, an enzyme which induces the resistance of those bacteria to penicillins, cephalosporins and, in severe infections, to penicillins with β-lactamase inhibitors. in this situation, the carbapenems become the drugs of choice. the objective of this study was to analyse the in vitro efficacy of three carbapenems: ertapenem, imipenem a ...201423877897
characterization and genomic analyses of two newly isolated morganella phages define distant members among tevenvirinae and autographivirinae subfamilies.morganella morganii is a common but frequent neglected environmental opportunistic pathogen which can cause deadly nosocomial infections. the increased number of multidrug-resistant m. morganii isolates motivates the search for alternative and effective antibacterials. we have isolated two novel obligatorily lytic m. morganii bacteriophages (vb_mmom_mp1, vb_mmop_mp2) and characterized them with respect to specificity, morphology, genome organization and phylogenetic relationships. mp1's dsdna ge ...201728387353
successful treatment of pd peritonitis due to morganella morganii resistant to third-generation cephalosporins - a case report.morganella morganii is a rare cause of peritonitis in patients on peritoneal dialysis (pd). most of the reported cases have resorted to a switch to hemodialysis. we herein report a case of peritonitis due to m. morganii resistant to third-generation cephalosporins, which was treated successfully with intraperitoneal (ip) tobramycin followed by oral ciprofloxacin. early microbiologic diagnosis is essential in the treatment of peritonitis from rare microorganisms such as morganella morganii, and a ...201728360373
a case of sulfhemoglobinemia in a child with chronic constipation.sulfhemoglobinemia is a rare condition in which a sulfur atom oxidizes the heme moiety in hemoglobin, making the hemoglobin incapable of carrying oxygen and leading to hypoxia and cyanosis. this condition has been described in patients taking sulfur medications or who have cultured hydrogen sulfide producing intestinal bacteria such as morganella morganii. this case describes a pediatric patient who was found to have cyanosis on two occasions of urinary tract infection in the setting of chronic ...201728348950
fatal rhabdomyolysis caused by morganella morganii in a patient with multiple myeloma.a 64-year-old japanese man with multiple myeloma was admitted to our institute due to fever and hypotension. he had received multiple courses of chemotherapy just before his febrile episode. blood culturing detected morganella morganii. at the time of the diagnosis, his laboratory findings revealed massive rhabdomyolysis with a significantly increased creatinine kinase level (ck; 3,582 u/l); 98.8% of which corresponded to the ck-mb isotype. we diagnosed the patient with sepsis caused by m. morga ...201728154285
biofilm in osteomyelitis caused by a rare pathogen, morganella morganii : a case report.morganella morganii is a member of enterobacteriaceae family, whose natural habitat is the human gastrointestinal tract. it rarely causes infection alone and is generally encountered in immunosuppressed patients. osteoarticular pathologies are not commonly observed with morganella morganii and infections by it have high mortality rate. biofilm colonization is a causative factor behind the chronicity and/or refractoriness of certain infections. biofilms colonize on inert medical devices, prosthes ...201627504288
severe chronic osteomyelitis caused by morganella morganii with high population diversity.a case of chronic osteomyelitis probably caused by morganella morganii, occurring over a period of 30 years, is reported. the organism was identified through a combination of sample culture, direct sequencing, and 16s rna gene amplicon sequencing. further whole-genome sequencing and population structure analysis of the isolates from the patient showed the bacterial population to be highly diverse. this case provides a valuable example of a long-term infection caused by an opportunistic pathogen, ...201627495005
persistent infection with metallo-beta-lactamase and extended spectrum β-lactamase producer morganella morganii in a patient with urinary tract infection after kidney transplantation.organ transplant recipients under immunosuppressive therapy have a highly increased risk of acquiring unusual opportunistic infections. diagnosis of the etiology of infection may be difficult in clinical manifestations, which need further histological and biological investigations. here in we report, for the 1(st) time in the iran, a morganella morganii isolate harboring blavim, blactx-m, and blashv genes after kidney transplantation with persistent urinary infections.201727433071
morganella morganii, a non-negligent opportunistic pathogen.morganella morganii belongs to the tribe proteeae of the enterobacteriaceae family. this species is considered as an unusual opportunistic pathogen that mainly causes post-operative wound and urinary tract infections. however, certain clinical m. morganii isolates present resistance to multiple antibiotics by carrying various resistant genes (such as blandm-1, and qnrd1), thereby posing a serious challenge for clinical infection control. moreover, virulence evolution makes m. morganii an importa ...201627421818
morganella morganii bacteria produces phenol as the sex pheromone of the new zealand grass grub from tyrosine in the colleterial gland.costelytra zealandica (coleoptera: scarabeidae) is a univoltine endemic species that has colonised and become a major pest of introduced clover and ryegrass pastures that form about half of the land area of new zealand. female beetles were previously shown to use phenol as their sex pheromone produced by symbiotic bacteria in the accessory or colleterial gland. in this study, production of phenol was confirmed from the female beetles, while bacteria were isolated from the gland and tested for at ...201627352077
molecular investigation of extended-spectrum beta-lactamase genes and potential drug resistance in clinical isolates of morganella morganii.resistance to beta-lactam antibiotics has become more common in morganella morganii, which can cause of outbreaks of bacteremia and septicemia in postoperative patients.201727236395
characterization of integrons and qnr genes in proteeae from a teaching hospital in china.proteeae isolates displaying multidrug-resistance (mdr) are the second most common causes of hospital-associated infections. the aim of this study was to screen class 1-3 integrons and plasmid-mediated quinolone resistance (pmqr) genes in proteeae isolates from the first affiliated hospital of the wenzhou medical university.201727220322
a severe morganella morganii endophthalmitis; followed by bacteremia.morganella morganii is rarely isolated from nosocomial infections. however, postoperative infections due to morganella spp. were documented in literature and eye involvements of the infections usually result in severe sequels. we present a severe case infection, which was caused by m. morganii subsp. morganii, firstly appearing as conjunctivitis and complicated by bacteremia. the infectious agent isolated from both conjunctival and consecutive blood cultures. identification and antimicrobial sus ...201627092227
inhibition of morganella morganii histidine decarboxylase activity and histamine accumulation in mackerel muscle derived from filipendula ulumaria extracts.filipendula ulmaria, also known as meadowsweet, is an herb; its extract was examined for the prevention of histamine production, primarily that caused by contaminated fish. the efficacy of meadowsweet was assessed using two parameters: inhibition of morganella morganii histidine decarboxylase (hdc) and inhibition of histamine accumulation in mackerel. ellagitannins from f. ulmaria (rugosin d, rugosin a methyl ester, tellimagrandin ii, and rugosin a) were previously shown to be potent inhibitors ...201626939657
[the great imitator. case report].authors present two patients suffering from renal tuberculosis, which caused differential diagnostic problems. the first patient was examined because of fever and left flank pain. computed tomography revealed renal shrinkage on the left side. retrograde pyelography demonstrated ureteric stricture and dilated calices. urine culture showed mycobacterium tuberculosis. two months after initiation of the antituberculotic therapy nephrectomy was performed. the second patient was referred to the depart ...201626895803
septic shock induced by bacterial prostatitis with morganella morganii subsp. morganii in a posttransplantation patient.bacterial infection is a common complication after hematopoietic stem cell transplantation (hsct). morganella morganii is ubiquitous gram-negative facultative anaerobe, which may cause many kinds of opportunistic infection. herein we report a case of a 55-year-old man who presented with frequent urination, urgency, and mild pain that comes and goes low in the abdomen and around the anus. the patient had a medical history of chronic prostatitis for 4 years. he received hla-matched sibling allo-hs ...201526798544
morganella morganii peritonitis associated with continuous ambulatory peritoneal dialysis (capd) after colonoscopy.a 79-year-old man on continuous ambulatory peritoneal dialysis (capd) developed abdominal pain and cloudy peritoneal fluid two days after colonoscopy that revealed multiple diverticula. the white blood cell count was 9,000 cells/μl, c-reactive protein level was 6.86 mg/dl, and the white blood cell count of the peritoneal fluid was 7,800 cells/μl, suggesting acute peritonitis. empiric therapy consisting of cefazolin and ceftazidime slowly improved the patient's symptoms. the initial microbiologic ...201626781017
multiple liver abscesses due to morganella morganii. 201526574995
neonatal sulfhemoglobinemia and hemolytic anemia associated with intestinal morganella morganii.sulfhemoglobinemia is a rare disorder characterized by the presence of sulfhemoglobin in the blood. it is typically drug-induced and may cause hypoxia, end-organ damage, and death through oxygen deprivation. we present here a case of non-drug-induced sulfhemoglobinemia in a 7-day-old preterm infant complicated by hemolytic anemia. microbiota compositional analysis of fecal samples to investigate the origin of hydrogen sulphide revealed the presence of morganella morganii at a relative abundance ...201526553186
draft genome sequence of a diarrheagenic morganella morganii isolate.this is a report of the whole-genome draft sequence of a diarrheagenic morganella morganii isolate from a patient in michigan, usa. this genome represents an important addition to the limited number of pathogenic m. morganii genomes available.201526450735
a rapidly progressing, deadly disease of actias selene (indianmoonmoth) larvae associated with a mixed bacterial and baculoviral infection.the outbreak of an infectious disease in captive-bred lepidoptera can cause death of all the caterpillars within days. a mixed baculoviral-bacterial infection observed among actias selene (hubner 1807), the indian moon moth (insecta: lepidoptera: saturniidae), larvae was characterized and followed by a photographic documentation of the disease progression. the etiological agents were determined using mass spectrometry and polymerase chain reaction (pcr). it appeared that the disease was caused b ...201526333395
mmosti restriction endonuclease, isolated from morganella morganii infecting a tropical moth, actias selene, cleaving 5'-|ccngg-3' sequences.a type ii restriction endonuclease, mmosti, from the pathogenic bacterium morganella morganii infecting a tropical moth, actias selene, has been detected and biochemically characterized, as a potential etiological differentiation factor. the described rease recognizes interrupted palindromes, i.e., 5'-ccngg-3' sequences and cleaves dna leaving 5-nucleotide (nt) long, single-stranded (ss), 5'-cohesive ends, which was determined by three complementary methods: (i) cleavage of custom and standard d ...201626280518
draft whole-genome sequence of morganella morganii serotype o:1ab.morganella morganii is a facultative pathogen of humans, causing urinary tract and postsurgical infections. here, we report a high-quality draft assembly of the o:1ab serotype.201525999571
[new metalloendopeptidase of morganella morganii zm].proteolytic activity which is inhibited in the presence of o-phenanthroline was found in m. morganii zm. intracellular proteases of m. morganii zm unlimited split musculoskeletal actin in contrast to grimelysin. several proteolitic proteins of m. morganii zm cells were identified by zymography with gelatin. metalloproteinase of m. morganii zm cell lysate was purified by hydrophobic chromatography fractionation. the molecular weight of the protein was 35 kda.201525895364
development of a real-time pcr method coupled with a selective pre-enrichment step for quantification of morganella morganii and morganella psychrotolerans in fish products.histamine fish poisoning is common and due to toxic concentrations of histamine often produced by gram-negative bacteria in fin-fish products with a high content of the free amino acid histidine. the genus morganella includes two species previously reported to cause incidents of histamine fish poisoning. morganella morganii and morganella psychrotolerans are both strong producer of histamine. however, little is known about the occurrence and critical stages for fish contamination with these bact ...201525791250
hemorrhagic stomatitis in a natural hybrid of vipera ammodytes × vipera berus due to inappropriate substrate in terrarium.a natural hybrid of vipera ammodytes × vipera berus was presented having low body weight, seizures and generalized swelling of the cephalic region. based on the history of the case and clinical examination, hemorrhagic stomatitis of traumatic origin was diagnosed. the snake was kept in a terrarium with wood chips as a substrate, and the material had induced trauma in the oral mucosa which was further complicated with salmonella arizonae and morganella morganii co-infection, abscessation and oste ...201525715871
the structure of the morganella morganii lipopolysaccharide core region and identification of its genomic loci.the core region of the lipopolysaccharide of morganella morganii serotype o:1ab was obtained by hydrolysis of the lps and studied by 2d nmr, esi ms, and chemical methods. its structure was highly homologous to those from the two major members of the same proteeae tribe, proteus mirabilis and providencia alcalifaciens, and analysis of the m. morganii genome disclosed that the loci for its outer core, lipid a and ara4n moieties are similarly conserved.201525498024
sepsis caused by new delhi metallo-β-lactamase (blandm-1) and qnrd-producing morganella morganii, treated successfully with fosfomycin and meropenem: case report and literature review.the objective of this study was to describe the microbiological characteristics of an extensively drug-resistant (xdr) isolate of morganella morganii obtained from a patient with sepsis of urinary origin and to describe the patient's clinical characteristics. we further aimed to perform a literature review of the situation in latin america regarding gram-negative bacillus (gnb) carriers of new delhi metallo-β-lactamase (ndm-1) and qnr genes and current reports on the treatment of infections caus ...201525447717
analysis of plasmid-mediated quinolone resistance genes in clinical isolates of the tribe proteeae from argentina: first report of qnrd in the americas.to analyse the occurrence and prevalence of plasmid-mediated quinolone resistance (pmqr) genes in the tribe proteeae, 81 isolates (65 proteus spp., 12 morganella morganii and 4 providencia stuartii) consecutively collected in 66 hospitals belonging to the whonet-argentina resistance surveillance network were studied. of the 81 isolates, 50 (62%) were susceptible to quinolones [43/65 (66%) proteus spp. and 7/12 (58%) m. morganii). the remaining 31 isolates (22 proteus spp., 5 m. morganii and all ...201427873695
morganella morganii causing abscess over the anterior chest wall- a case report.a 17-year-old female college student presented with recurrent abscess over the anterior chest wall since one and half year. morganella morganii was isolated from the aspirated pus. patient was started on oral ciprofloxacin and the lesion resolved in two weeks.201425386434
effects of breakpoint changes on carbapenem susceptibility rates of enterobacteriaceae: results from the sentry antimicrobial surveillance program, united states, 2008 to 2012.in the absence of clinical resistance, breakpoints for many antimicrobial agents are often set high. clinical failures following use of the agents over time requires re-evaluation of breakpoints. this is based on patient response, pharmacokinetic/pharmacodynamic information and in vitro minimal inhibitory concentration data. data from the sentry antimicrobial surveillance program has shown that clinical and laboratory standards institute breakpoint changes for carbapenems that occurred between 2 ...201425371693
solitary large intestinal diverticulitis in leatherback turtles (dermochelys coriacea).leatherback sea turtles are globally distributed and endangered throughout their range. there are limited data available on disease in this species. initial observations of solitary large intestinal diverticulitis in multiple leatherbacks led to a multi-institutional review of cases. of 31 subadult and adult turtles for which complete records were available, all had a single exudate-filled diverticulum, as large as 9.0 cm in diameter, arising from the large intestine immediately distal to the il ...201525239052
characterization of a novel morganella morganii bacteriophage fsp1 isolated from river water.morganella morganii has been identified as a causative agent of opportunistic infections and histamine poisoning. bacteriophage is a virus and has recently been considered an alternative agent to antibiotics for the control of bacteria that have developed antibiotic resistance. in this study, a novel m. morganii bacteriophage isolated from river water was characterized. the isolated phage, termed fsp1, was purified by polyethylene glycol precipitation followed by cesium chloride density-gradient ...201425168469
clinical manifestations and prognostic factors of morganella morganii bacteremia.although morganella morganii causes a variety of clinical infections, there are limited studies on m. morganii bacteremia after the year 2000. a total of 109 patients with m. morganii bacteremia at a medical center in taiwan from 2003 to 2012 were studied. among them, 30.3 % had polymicrobial bacteremia and 75.2 % had community-acquired infection. the most common underlying diseases were hypertension (62.4 %) and diabetes mellitus (38.5 %). the urinary tract (41.3 %) was the major portal of entr ...201525107625
genome analysis of ndm-1 producing morganella morganii clinical isolate.to analyze the resistome and virulence genes of morganella morganii f675, a multidrug-resistant clinical isolate using whole genome sequencing (wgs).201425081858
[purification and characterization of esterase from morganella morganii zjb-09203].enantioselective hydrolysis of 2-carboxyethyl-3-cyano-5-methylhexanoic acid (cnde) is the key step in chemoenzymatic synthesis of pregabalin. we purified an intracellular carboxyl esterase from morganella morganii zjb-09203, which exhibited high enantioselectivity and activity towards cnde. the carboxyl esterase was purified to electrophoretic homogeneity by ammonium sulfate fraction precipitation, phenyl sepharose 6 ff hydrophobic interaction chromatography, anion exchange with deae sephadex a- ...201424818487
trends of isolation of intrinsically resistant to colistin enterobacteriaceae and association with colistin use in a tertiary hospital.the objective of this investigation was to evaluate the association between colistin consumption and the isolation of intrinsically resistant to colistin enterobacteriaceae (irce) in a university hospital in crete, greece. the database of the microbiological laboratory was reviewed retrospectively during 2006-2010. all positive cultures for irce were retrieved. we assessed the total consumption of colistin in medical, surgical, and intensive care units (icus). a total of 1,304 single-patient irc ...201424798249
inhibitory effects of brown algae extracts on histamine production in mackerel muscle via inhibition of growth and histidine decarboxylase activity of morganella morganii.this study was performed to investigate the inhibitory effects of brown algae extracts on histamine production in mackerel muscle. first, antimicrobial activities of brown algae extracts against morganella morganii were investigated using a disk diffusion method. an ethanol extract of ecklonia cava (ecee) exhibited strong antimicrobial activity. the minimum inhibitory concentration (mic) of ecee was 2 mg/ml. furthermore, the brown algae extracts were examined for their ability to inhibit crude h ...201424394193
intratesticular abscess in a solitary testicle: the case for testicle sparing management.we present the case of a 24-year-old immunocompromised man with an intratesticular abscess. the patient presented with one week of left scrotal pain and swelling. workup included scrotal ultrasonography which revealed a large fluid collection within the tunica albuginea of the left testis. surgical exploration of the left testis evacuated a significant amount of purulent fluid. the residual viable testicular parenchyma was salvaged. intraoperative cultures grew morganella morganii. follow-up ult ...201324198833
morganella morganii pericarditis in a patient with multiple myeloma.purulent pericarditis caused by morganella morganii is extremely rare. we report herein a case of a 61-year-old man who presented with chest pain and dyspnea fourteen days after chemotherapy for multiple myeloma. echocardiogram and computed tomography revealed a massive pericardial effusion and associated cardiac tamponade. pericardiocentesis was performed. pericardial fluid was found to be purulent, and morganella morganii was isolated from the fluid. the patient was successfully treated with a ...201324106621
[a case of septic shock and multiple organ injury induced by urinary tract infection with morganella morganii subsp. morganii]. 201324059431
more on peritonitis by morganella morganii. 201523843599
prevalence of quinolone resistance determinant qnra6 among broad- and extended-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates with sul1-type class 1 integron association in a tunisian hospital.the aim of this study was to investigate the prevalence and the emergence of plasmid-mediated quinolone resistance among broad-spectrum beta-lactam-resistant proteus mirabilis and morganella morganii clinical isolates recovered in the military hospital in tunisia.201323746343
catfish spine envenomation and bacterial abscess with proteus and morganella: a case report.abscess formation and cellulitis in the setting of envenomation are rare complications of handling catfish. to the best of our knowledge, isolation of proteus vulgaris has not been previously recorded, and recovery of morganella morganii has been reported in only one prior case from wound cultures in patients injured by catfish stings. we report a case of catfish envenomation characterized by abscess formation and cellulitis, in which wound cultures grew these unusual organisms.201323631594
bioautography-guided isolation of antibacterial compounds of essential oils from thai spices against histamine-producing bacteria.the outbreak of histamine fish poisoning has been being an issue in food safety and international trade. the growth of contaminated bacterial species including morganella morganii which produce histidine decarboxylase causes histamine formation in fish during storage. histamine, the main toxin, causes mild to severe allergic reaction. at present, there is no well-established solution for histamine fish poisoning. this study was performed to determine the antibacterial activity of essential oils ...201323625419
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