| absence of autoantibodies to peptides shared by hla-b27.5 and klebsiella pneumoniae nitrogenase in serum samples from hla-b27 positive patients with ankylosing spondylitis and reiter's syndrome. | some microorganisms which are pathogenic in humans share amino acid sequences with human proteins (molecular mimicry). it has been suggested that molecular mimicry might be a reason for autoimmunity as a result of immunological cross reactivity. a homologous sequence of six amino acids has been found in both klebsiella pneumoniae nitrogenase and the hla-b27.5 molecule. in addition, (auto)antibodies to a synthetic peptide that contained the hla-b27.5/klebsiella mimicking epitope have been detecte ... | 1992 | 1616364 |
| review of the in vitro antibacterial activity of cefprozil, a new oral cephalosporin. | cefprozil is a newer oral cephalosporin with a spectrum of activity against organisms that include gram-positive and gram-negative pathogens. a review of published data shows that cefprozil is active (susceptibility, less than or equal to 8 micrograms/ml; moderate susceptibility, 16 micrograms/ml; resistance, greater than or equal to 32 micrograms/ml) against gram-positive species such as streptococci, methicillin-susceptible staphylococci, and listeria monocytogenes; it may have marginal activi ... | 1992 | 1617037 |
| experimental autoimmune adrenalitis: a murine model for addison's disease. | experimental autoimmune adrenalitis was produced in mice by immunizing 8 times or more at intervals of 30 days with syngeneic adrenal extract mixed with klebsiella o3 lipopolysaccharide (ko3 lps) as a potent adjuvant. the cortex regions of the adrenal glands after the 8th injection were definitely infiltrated with polymorphonuclear leukocytes (pmn). the main infiltrates in the lesions after the 9th injection were replaced by mononuclear cells, such as small lymphocytes and macrophages, and furth ... | 1992 | 1617104 |
| in vitro activity of loracarbef and effects of susceptibility test methods. | loracarbef is a new oral antimicrobial of the carbacephem class with in vitro activity against the common pathogens associated with skin infections, otitis media, sinusitis, bronchopulmonary infections, and urinary tract infections. a review of the literature shows the following ranges for 90% minimum inhibitory concentration (mic90) values (microgram/ml) against the organisms that commonly cause these illnesses: streptococcus pneumoniae, 0.25-2.0; moraxella (branhamella) catarrhalis (beta-lacta ... | 1992 | 1621748 |
| hemicellulose bioconversion to polyanionic heteropolysaccharides. | anionic polysaccharides, traditionally obtained from plant or algal sources, have a variety of commercial uses. such gums from microorganisms have received increased recent interest. we have initiated a program to investigate the bioconversion of pentosans to rheologically useful anionic extracellular polysaccharides (aeps). a number of earlier-described species, including cryptococcus laurentii, klebsiella pneumoniae, arthrobacter viscosus, and pseudomonas atcc 31260, appear to have potential i ... | 1992 | 1622201 |
| enhancement of 1,3-propanediol production by cofermentation in escherichia coli expressing klebsiella pneumoniae dha regulon genes. | 1,3-propanediol (1,3-pd) is an intermediate in chemical and polymer synthesis. we have previously expressed the genes of a biochemical pathway responsible for 1,3-pd production, the dha regulon of klebsiella pneumoniae, in escherichia coli. an analysis of the maximum theoretical yield of 1,3-pd from glycerol indicates that the yield can be improved by the cofermentation of sugars, provided that kinetic constraints are overcome. the yield of 1,3-pd from glycerol was improved from 0.46 mol/mol wit ... | 1992 | 1622202 |
| excretion of ammonium by a nifl mutant of azotobacter vinelandii fixing nitrogen. | a mutation in the gene upstream of nifa in azotobacter vinelandii was introduced into the chromosome to replace the corresponding wild-type region. the resulting mutant, mv376, produced nitrogenase constitutively in the presence of 15 mm ammonium. when introduced into a nifh-lacz fusion strain, the mutation permitted beta-galactosidase production in the presence of ammonium. the gene upstream of nifa is therefore designated nifl because of its similarity to the klebsiella pneumoniae nifl gene in ... | 1992 | 1622243 |
| 1,3-propanediol:nad+ oxidoreductases of lactobacillus brevis and lactobacillus buchneri. | in the cofermentation of glycerol with a sugar by lactobacillus brevis and lactobacillus buchneri, a 1,3-propanediol:nad+ oxidoreductase provides an additional method of nadh disposal. the enzyme has been purified from both l. brevis b22 and l. buchneri b190 and found to have properties very similar to those reported for the enzyme from klebsiella pneumoniae. the enzymes required mn2+ and are probably octamers with a molecular mass of 350 kda. although not absolutely specific for 1,3-propanediol ... | 1992 | 1622279 |
| gram negative aerobic bacillus pneumonia in oral and maxillofacial surgery--a case for comment. | a case illustrating the potentially fatal complication of endogenous gram negative aerobic bacillus (gnab) septicaemia secondary to nosocomial pneumonia is presented along with current theories as to its aetiology. the technique of selective decontamination of the digestive tract is designed and advocated to prevent such occurrences; oral and maxillofacial surgeons should be aware of this approach. it may be, however, that by using much simpler manoeuvres such as changes in policy regarding gast ... | 1992 | 1622960 |
| does high level production of shv-type penicillinase confer resistance to ceftazidime in enterobacteriaceae? | we report the isolation of a clinical isolate of klebsiella pneumoniae that showed resistance to ceftazidime (mic: 8 micrograms/ml), susceptibility to aztreonam (mic: 2 micrograms/ml) and cefotaxime (mic: 0.015 micrograms/ml). a synergistic effect between clavulanic acid and ceftazidime or aztreonam against this strain was also observed. the strain hyperproduced shv-1 penicillinase (990 u/g) which is encoded by a self-transferrable plasmid of at least 150 kb. that the ceftazidime-resistance phen ... | 1992 | 1624115 |
| calculous pyelonephritis. | the authors report on 48 patients with calculous pyelonephritis. urinary obstruction was caused by renal calculi in 21 patients and ureteral calculi in 27. urine cultures were positive in 87.5% and bacteremia was seen in 70%. the common organisms in urine and blood culture were e. coli, proteus and klebsiella. septic shock occurred in 10 (20.8%) out of 48 patients. calculous pyelonephritis with urinary obstruction is a very serious condition. | 1992 | 1624252 |
| cholangiohepatitis in a dog. | cholangiohepatitis was diagnosed in a dog with a 4-day history of anorexia, vomiting, fever, and icterus. additional findings included signs of depression, dehydration, hepatosplenomegaly, and abdominal discomfort. exploratory laparotomy was performed, and specimens of liver, spleen, and bile were obtained. histologic evaluation of liver and spleen revealed acute, suppurative cholangio-hepatitis and splenitis, respectively. cultures of liver and bile yielded klebsiella sp. the dog responded to r ... | 1992 | 1624352 |
| a novel plasmid-mediated extended-spectrum beta-lactamase not derived from tem- or shv-type enzymes. | | 1992 | 1624397 |
| klebsiella aerogenes urease gene cluster: sequence of ured and demonstration that four accessory genes (ured, uree, uref, and ureg) are involved in nickel metallocenter biosynthesis. | the region located immediately upstream from the klebsiella aerogenes urease structural genes was sequenced and shown to possess an open reading frame capable of encoding a 29.8-kda peptide. deletions were generated in this gene, denoted ured, and in each of the genes (uree, uref, and ureg) located immediately downstream of the three structural genes. transformation of the mutated plasmids into escherichia coli resulted in high levels of urease expression, but the enzyme was inactive (deletions ... | 1992 | 1624427 |
| [experimental study on development and lung injury in pneumonia under neutropenic condition]. | this study was performed to demonstrate the characteristic findings in pneumonia under neutropenic condition. the results were as follows. 1) after nebulizing klebsiella pneumonia dt-s strains, the survival rate in the neutropenic mouse group rapidly decreased compared with that of the control group. 2) in the neutropenic state, a rapid decline of survival rate was demonstrated during the initial stage of infection. bacteremia and endotoxemia developed earlier than in the healthy control group. ... | 1992 | 1625394 |
| [acute suppurative thyroiditis and klebsiella pneumoniae sepsis. a case report and review of the literature]. | the acute suppurative thyroiditis is a rare infection. it affects specially patients with preexisting thyroid gland pathology and its frequency is higher in women. in childhood it's linked to local anatomic defects. the infection used to be located in left thyroid lobe and it's much less usual in right lobe, in both or in isthmus. the most important causal microorganisms are staphylococci (staphylococcus aureus overcoat) and streptococci (usually streptococcus pyogenes and streptococcus pneumoni ... | 1992 | 1626091 |
| evaluation of sulbactam plus ampicillin for treatment of experimentally induced klebsiella pneumoniae lung infection in foals. | efficacy of sulbactam, a beta-lactamase inhibitor, in combination with ampicillin, was evaluated for treatment of experimentally induced pneumonia caused by beta-lactam-resistant klebsiella pneumoniae. infection was experimentally induced in 18 healthy weanling foals that were randomly allocated to 3 treatment groups: sulbactam plus ampicillin (s/a, 3.3 and 6.6 mg/kg of body weight, respectively), ampicillin (6.6 mg/kg), or vehicle only. foals were treated daily for 7 days; the observer was unaw ... | 1992 | 1626775 |
| effect of pine seed shell extract on microbial and viral infection. | pretreatment of mice with ammonia extract of seed shell of pinus koraicenis, via the intraperitoneal or intravenous route, effectively protected them from lethal infection of escherichia coli, klebsiella pneumoniae and staphylococcus aureus. the pine seed shell extract also moderately inhibited syncytium formation and cytopathogenic effect induced by human immunodeficiency virus (hiv) infection in cultured human lymphotropic virus type i (htlv-1) positive mt-4 cells. these data suggest a medicin ... | 1992 | 1627738 |
| monocyte cytokine secretion induced by chemically-defined derivatives of klebsiella pneumoniae. | the capacity of a k. pneumoniae membrane proteoglycan (kp-mpg) and four of its chemically defined derivatives to activate human monocytes was studied by measuring immunoreactive il-1 beta, il-6 and tumour necrosis factor-alpha (tnf-alpha) in culture supernatants. monocyte culture supernatants were also tested for their comitogenic activity on concanavalin a-stimulated thymocytes and for their cytotoxic activity on the mouse fibroblastic l929 cell line. the four kp-mpg derivatives were: (i) an ac ... | 1992 | 1628418 |
| mechanism of na(+)-dependent citrate transport in klebsiella pneumoniae. | citrate transport via cits of klebsiella pneumoniae has been shown to depend on the presence of na+. this transport system has been expressed in escherichia coli, and uptake of citrate in e. coli membrane vesicles via this uptake system was found to be an electrogenic process, although the ph gradient is the main driving force for citrate uptake (m. e. van der rest, r. m. siewe, t. abee, e. schwartz, d. oesterhelt, and w. n. konings, j. biol. chem. 267:8971-8976, 1992). analysis of the affinity ... | 1992 | 1629151 |
| an unusual presentation of ischiorectal abscess. | | 1992 | 1630005 |
| nosocomial urinary tract infections at a pediatric hospital. | although the epidemiology of nosocomial urinary tract infections (nutis) is well-described in the adult population, there is little information on children. we therefore reviewed all our patients with nutis, to determine their age, underlying diagnoses, microorganisms involved, use of catheters and secondary bacteremias. during a 2-year period at our institution, nutis accounted for 10% of all nosocomial infections (0.8 infections/100 admissions). of 44,948 patients admitted during this period 0 ... | 1992 | 1630854 |
| [emphysematous pyelonephritis. a serious complication of diabetes mellitus]. | pyelonephritis emphysematous (pe) is a life threatening renal infection which is observed practically exclusively as a serious complication of diabetes mellitus. 95% of the 73 cases which have been reviewed were found in diabetic patients. the symptomatology resembles that of severe acute pyelonephritis but the disease differs from this in that, in pe, emphysema develops in the actual renal parenchyma and/or in the perirenal tissues. the most important single factor in the etiology appears to be ... | 1992 | 1631968 |
| the predominant microflora of the palatal mucosa in an elderly island population. | the prevalence and microbiology of macrophotographically documented denture stomatitis were studied in denture wearers participating in an interdisciplinary health-monitoring project (koster health project) on the koster islands, sweden. upper dentures were used by 26.6% of the adult population, and 59.2% of the denture wearers had stomatitis. denture stomatitis type i was identified as sialadenitis. the more severe forms of denture stomatitis (types ii and iii) demonstrated increased recovery o ... | 1992 | 1632207 |
| close amino acid sequence relationship between the new plasmid-mediated extended-spectrum beta-lactamase men-1 and chromosomally encoded enzymes of klebsiella oxytoca. | isolated from an escherichia coli strain men-1 is a plasmid-mediated beta-lactamase that confers resistance to methoxy imino third-generation cephalosporins. the protein purified to homogeneity was digested by trypsin, chymotrypsin and endoproteinase asp-n. amino acid sequence determinations of the resulting peptides gave rise to the alignment of the 263 residues of the beta-lactamase. from amino acid sequence comparison men-1 was found to share more than 72% identity with the chromosomally medi ... | 1992 | 1633193 |
| infections in acute myeloid leukemia. study of 184 febrile episodes. | we analysed the case records of 75 patients with acute myeloid leukaemia treated at our institute from january 1984 to december 1988 to see the pattern and severity of infections and their relationship with granulocytopenia. a total of 184 febrile episodes (mean 2.45) were recorded; 153 (83.15%) were associated with granulocytopenia while 31 (16.84%) were without granulocytopenia. among granulocytopenic patients, infections could be documented microbiologically in 58.2% and clinically in 30.0% o ... | 1992 | 1634456 |
| potential for local and systemic bacterial infection in some occupational groups in benin city, nigeria. | many third world countries may not have adequate facilities for rapid sensitivity testing of antibiotics as an aid to diagnosis and chemotherapy. it may therefore be valuable to relate bacterial carriage on the skin to type of work a person does. | 1992 | 1636931 |
| ethanol production from cellobiose, amorphous cellulose, and crystalline cellulose by recombinant klebsiella oxytoca containing chromosomally integrated zymomonas mobilis genes for ethanol production and plasmids expressing thermostable cellulase genes from clostridium thermocellum. | the zymomonas mobilis genes for ethanol production have been integrated into the chromosome of klebsiella oxytoca m5a1. the best of these constructs, strain p2, produced ethanol efficiently from cellobiose in addition to monomeric sugars. utilization of cellobiose and cellotriose by this strain eliminated the requirement for external beta-glucosidase and reduced the amount of commercial cellulase needed to ferment solka floc sw40 (primarily crystalline cellulose). the addition of plasmids encodi ... | 1992 | 1637151 |
| ofloxacin versus trimethoprim/sulfamethoxazole in the treatment of uncomplicated urinary tract infection. | a multicenter randomized study was conducted to compare the efficacy and safety of ofloxacin with that of trimethoprim/sulfamethoxazole (tmp/smx) in the treatment of uncomplicated urinary tract infection in adults. patients were randomized to receive either oral ofloxacin 200 mg daily for three days (102 patients), or oral tmp/smx 160 mg/800 mg twice daily for seven days (100 patients). the pathogen was eradicated in 73 (97.3%) of the 75 evaluable patients receiving ofloxacin and in 66 (97.1%) o ... | 1992 | 1638586 |
| identification of enap-1, an antimicrobial peptide from equine neutrophils. | endogenous, cysteine-rich antimicrobial peptides known as defensins are prominent components of human, rabbit, and rat neutrophils, yet little is known about their occurrence in other mammalian species. although we did not detect mature (i.e., processed) defensins in equine neutrophil granules, we found that these granules contained small amounts of other cysteine-rich peptides with antimicrobial activity. one of these, enap-1, was purified by a combination of gel permeation and reversed-phase h ... | 1992 | 1639474 |
| stripping interfering sugars from samples using adapted bacteria. | bacteria adapted to individual sugars quickly remove targeted sugars--stripping them--from samples in which unwanted sugars interfere. adapted bacteria are equivalent to specific reagents for removal of sugars down to bacterial km values, micromolar to submicromolar concentrations. bacterial stripping is a simple method, useful when background sugars in micro-to millimolar concentrations (or larger) interfere with analysis of sought-for sugars. bacteria such as escherichia coli and klebsiella ar ... | 1992 | 1640061 |
| the aeromonas hydrophila exee gene, required both for protein secretion and normal outer membrane biogenesis, is a member of a general secretion pathway. | the aeromonas hydrophila tn5-751 insertion mutant l1.97 is unable to secrete extracellular proteins, and is fragile because of defective assembly of its outer membrane. a kpni 4.1 kb fragment, which complements this mutant when supplied with an exogenous promoter, was isolated and sequenced. it contains two complete genes, exee and exef, plus fragments of two others and may form part of an operon. the exee and exef open reading frames encode 501-residue m(r) 55,882 and 388-residue m(r) 43,431 pr ... | 1992 | 1640836 |
| specific binding of the transcription factor sigma-54 to promoter dna. | a central event in transcription is the assembly on dna of specific complexes near the initiation sites for rna synthesis. activation of transcription by one class of enhancer-binding proteins requires an rna polymerase holoenzyme containing the specialized transcription factor, sigma-54 (sigma 54). we report here that sigma 54 alone specifically binds to promoter dna and is responsible for many of the close contacts between rna polymerase holoenzyme and promoter dna, a property proposed for the ... | 1992 | 1641025 |
| identification of a lacz gene in vibrio cholerae. | plasmids that express an enzymatically active beta-galactosidase in an escherichia coli delta lac strain were isolated from libraries of recombinant plasmids containing vibrio cholerae chromosomal dna. deletion analysis localized the gene responsible for beta-galactosidase activity on a 3.1-kb dna fragment, and the gene product was identified as a protein of approximately 110 kda. primary sequence comparisons indicated that this v. cholerae gene is homologous to the e. coli lacz gene. in contras ... | 1992 | 1641510 |
| ofloxacin, a new broad-spectrum fluoroquinolone. results from a multicenter, national comparative activity surveillance study. the ofloxacin surveillance group. | ofloxacin, a newer broad-spectrum fluoroquinolone, was evaluated against 6967 clinical isolates in a multicenter surveillance trial using a standardized disk diffusion method. thirty-five geographically diverse laboratories contributed zone diameter results for two (ofloxacin and ciprofloxacin) to five (ofloxacin, ciprofloxacin, ampicillin, cefaclor, and cefixime) antimicrobial agents, depending on the site of infection. ofloxacin was determined to have the widest spectrum of activity and potent ... | 1992 | 1643819 |
| in vitro and in vivo antibacterial activities of meropenem, a new carbapenem antibiotic. | the in vitro and in vivo antibacterial activities of meropenem were compared with those of imipenem, ceftazidime, flomoxef, cefuzonam and cefotiam. meropenem showed a broad antibacterial spectrum against clinical isolates of gram-positive and gram-negative bacteria. against gram-negative bacteria, with the exception of acinetobacter calcoaceticus, meropenem exhibited the most potent activity among the drugs tested. it inhibited all 330 strains of enterobacteriaceae at 0.78 mg/l. meropenem was se ... | 1992 | 1644010 |
| cloning, sequencing and heterologous expression of a klebsiella pneumoniae gene encoding an fad-independent acetolactate synthase. | the gene encoding the valine-resistant and fad-independent acetolactate synthase of klebsiella pneumoniae was isolated and expressed in escherichia coli. the nucleotide sequence of this gene was determined and it exhibited an open reading frame of 1680 bp in length. in vivo expression of the acetolactate synthase-encoding gene in e. coli revealed a single 60-kda protein which is consistent with the molecular weight calculated from the deduced amino acid sequence of the gene product. the gene pro ... | 1992 | 1644303 |
| peptides related to the carboxyl terminus of human platelet factor iv with antibacterial activity. | a peptide (c13) corresponding to the last 13 amino acids of the carboxyl terminus of human platelet factor iv was found to be antibacterial. amino acid substitutions predicted to disrupt either the amphipathic or alpha-helical nature of c13 rendered the peptide inactive. antibacterial activity was demonstrated in normal human serum on bacteria which had been previously exposed to low levels of cefepime, a beta-lactam antibiotic. peptide analogues were examined for more potent antibacterial activ ... | 1992 | 1644916 |
| in-vitro evaluation of ampicillin/brobactam and comparison with other beta-lactam antibiotics. | the ability of brobactam to inhibit beta-lactamases and the in vitro activity of ampicillin combined with brobactam was compared with another beta-lactamase inhibitor, clavulanic acid, and other beta-lactam antibiotics. both inhibitors showed good and similar activity against staphylococcal penicillinase and most broad-spectrum beta-lactamases found in the enterobacteriaceae, whether plasmid or chromosomally mediated. both inhibitors were less active against chromosomally mediated cephalosporina ... | 1991 | 1646779 |
| antitumor, antiviral and immunopotentiating activities of pine cone extracts: potential medicinal efficacy of natural and synthetic lignin-related materials (review). | several antitumor substances that effectively inhibited the growth of ascites and solid tumor cells transplanted in mice were isolated from pine cone naoh extract by acid- and ethanol-precipitation. these antitumor substances were also potent antiviral agents against human immunodeficiency virus, herpes simplex virus and influenza virus; they induced antimicrobial activity against staphylococcal aureus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae and candida albicans, and ind ... | 1991 | 1648335 |
| the combined use of lipoxygenase and cyclooxygenase inhibitors in klebsiella pneumoniae-induced bovine mastitis. | the effect of combined administration of flunixin meglumine (fm) and nordihydroguaiaretic acid (ndga) on milk prostaglandin f2 alpha (pgf2 alpha) and leukotriene b4 (ltb4) concentrations, and inflammatory indicators of bovine mastitis was examined. mastitis was induced in six holstein cows by the inoculation of klebsiella pneumoniae via the teat canal. four cows were intravenously treated with fm (1.1 mg/kg) and ndga (10 mg/kg) 1 hour prior to bacterial inoculation and again at post inoculation ... | 1991 | 1649514 |
| a sugar-specific porin, scry, is involved in sucrose uptake in enteric bacteria. | during the molecular analysis of a plasmid-coded sucrose metabolic pathway of enteric bacteria, a gene, scry, was found whose product, scry, had all the properties of a bacterial porin (schmid et al., 1988). loss of this protein (mr 58 kda), localized in the outer membrane, led, as shown here, to an increase in the apparent km for sucrose transport in whole cells from 10 microm in wild-type cells to 300 microm in mutant cells. this contrasts with the km for sucrose phosphorylation as measured in ... | 1991 | 1649946 |
| [pulmonary infections in renal transplant recipients]. | fifty-seven episodes of pulmonary infection occurred in 46 patients during a 11.5-year period, the incidence being 12% (57/475). after treatment, 22 patients recovered, and 24 died (52%). the etiology was determined in 47 infection episodes. 38(81%) of them were caused by bacteria, 6 by fungi, and 3 by tubercle bacilli. comparisons were made between infected and noninfected groups. the results showed that the incidence of rejection episodes, greatly elevated blood sugar level (greater than or eq ... | 1991 | 1650638 |
| [a two-year prospective survey on nosocomial infections]. | a two-year randomized prospective survey on nosocomial infections in hospitalized patients of hua shan hospital from 1985 to 1987 was carried out. altogether 1,826 patients were enrolled in the study, the incidence of nosocomial infection being 13.1%. the incidence in dermatology ward was the highest (19.8%), then in order of frequency; medical ward 16.5%, surgical ward 14.8%, ward of neurology 13.7%, ward of neurosurgery 12.7% etc. regarding the location of infection, lower respiratory tract in ... | 1991 | 1650640 |
| interactions of heavy metals with organisms and proteins. | voltammetric techniques [differential pulse polarography (dpp) and differential pulse anodic stripping voltammetry (dpasv)] have been used to determine, at various ph, conditional differential functions and average equilibrium constants of copper, zinc and lead with the bacteria klebsiella pneumoniae and methanosarcine, the alga selenastrum capricornutum printz and the proteins horse-heart cytochrome c and the soluble extract of sulphate-reducing bacteria. the buffer intensity in terms of metal ... | 1991 | 1652791 |
| site-specific integration of genes into hot spots for recombination flanking aada in tn21 transposons. | tn21-related transposons are widespread among bacteria and carry various resistance determinants at preferential sites, hs1 and hs2. in an in vivo integrative recombination assay it was demonstrated that these hot spots direct the integration of aminoglycoside resistance genes like aadb from klebsiella pneumoniae and aacai from serratia marcescens, in a reca- background. the maximum required recognition sequence which must be present in both the donor and recipient plasmids is 5' ctaaaacaaagtta ... | 1991 | 1654505 |
| construction of a novel suicide vector: selection for escherichia coli hb101 recombinants carrying the dna insert. | we constructed a new type of cloning vector, perish2, that transforms escherichia coli hb101 only when a foreign dna fragment is ligated into the cloning site of the plasmid vector. plasmid perish2 carries the rcsb gene which is derived from the chromosome of e. coli hb101 and is involved in the regulation of colanic acid production. when e. coli hb101 is transformed by this vector carrying the intact rcsb gene, the gene product rcsb blocks bacterial growth. however, if the rcsb gene is inactiva ... | 1991 | 1655577 |
| isolation of escherichia coli mutants defective in uptake of molybdate. | for the study of molybdenum uptake by escherichia coli, we generated tn5lac transposition mutants, which were screened for the pleiotropic loss of molybdoenzyme activities. three mutants a1, a4, and m22 were finally selected for further analysis. even in the presence of 100 microm molybdate in the growth medium, no active nitrate reductase, formate dehydrogenase, and trimethylamine-n-oxide reductase were detected in these mutants, indicating that the intracellular supply of molybdenum was not su ... | 1991 | 1655715 |
| klebsiella aerogenes catabolite gene activator protein and the gene encoding it (crp). | the catabolite gene activator protein from klebsiella aerogenes (capk) and the corresponding protein from escherichia coli (cape) were shown to be nearly identical. both capk and cape activated transcription from the cap-dependent promoters derived from e. coli and k. aerogenes. the crp gene from k. aerogenes (encoding cap) is tightly linked to rpsl. the nucleotide sequence of crp predicts an amino acid sequence for capk that differs in only one position from that of cape. | 1991 | 1655718 |
| multicenter evaluation of azithromycin and cefaclor in acute lower respiratory tract infections. | this was a randomized, third-party-blinded, multicenter study that compared once-daily azithromycin (500 mg on day 1, followed by 250 mg on days 2-5) to cefaclor (500 mg three times daily for 10 days) in the treatment of patients with acute bronchitis or pneumonia. a total of 546 patients were entered into the study and 272 patients were evaluable for efficacy analysis. of these, 249 (176 azithromycin, 73 cefaclor) had bronchitis and 23 (15 azithromycin, 8 cefaclor) had pneumonia. the combined c ... | 1991 | 1656740 |
| nosocomial spread of an amikacin resistance gene on both a mobilized, nonconjugative plasmid and a conjugative plasmid. | resistance to amikacin among members of the family enterobacteriaceae at a hospital in venezuela rose from 2% in 1979 to 5% in 1984 and 10% in 1985 as amikacin usage rose 20-fold to exceed gentamicin usage. resistance to gentamicin remained at 25 to 27%. we examined the plasmids from 21 isolates obtained in 1984 and 1985. nine of eleven in 1984 and three of ten in 1985 carried aaca and sul on a 3.8-kb bamhi fragment of pbwh300, a 10.4-kb nonconjugative plasmid that had been mobilized into strain ... | 1991 | 1656868 |
| klebsiella pneumoniae nitrogenase. the pre-steady-state kinetics of mofe-protein reduction and hydrogen evolution under conditions of limiting electron flux show that the rates of association with the fe-protein and electron transfer are independent of the oxidation level of the mofe-protein. | the pre-steady-state kinetics of h2 evolution from klebsiella pneumoniae nitrogenase functioning at 23 degrees c, ph 7.4, under conditions of extremely low electron flux through the mofe-protein exhibited a lag phase of several minutes duration. the approach to a steady-state rate of h2 evolution was accompanied by a 50% decrease in the amplitude of the mofe-protein e.p.r. signal. these kinetics have been simulated using our published kinetic model for nitrogenase [lowe & thorneley (1984) bioche ... | 1991 | 1656943 |
| presence of 5-methylcytosine in cc(a/t)gg sequences (dcm methylation) in dnas from different bacteria. | the presence of cc(a/t)gg sequences with methylated internal cytosine (dcm methylation) was determined in dna from different genera of eubacteria. this methylation was studied by using restriction enzymes ecorii and bstni, which cleave unmethylated or methylated cc(a/t)gg sequences. dcm methylation was only detected in genera of the family enterobacteriaceae closely related to escherichia: shigella, citrobacter, salmonella, and klebsiella. | 1991 | 1657894 |
| polyphosphoinositide metabolism in polymorphonuclear cells from healthy and thermally injured rats: effect of the immunomodulator ru 41740. | burn trauma is associated with alterations of various components of host defenses, including impaired neutrophil functions. in an animal model of experimental thermal injury, we studied if the modifications of cellular reactivity result from alterations in signalling systems by comparing polyphosphoinositide breakdown, particularly the production of inositol phosphates (ip, ip2 ip3), in healthy and burned rat polymorphonuclear neutrophil leukocytes (pmns). neutrophil activators such as n-formyl- ... | 1991 | 1658175 |
| subgingival microflora of advanced periodontitis in the dominican republic. | a study of the predominant subgingival microflora was carried out in 24 periodontitis patients, 18 to 60 years of age, in santo domingo, dominican republic. paper point sampling, transport in vmga iii, and conventional microbiological techniques were utilized. direct microscopic examination revealed that cocci and nonmotile organisms made up 85% of the total organisms and spirochetes as little as 3%. nonselective culturing showed gram-negative organisms to constitute 53% of total isolates. fusob ... | 1991 | 1658290 |
| substitutions at a single amino acid residue in the nitrogen-regulated activator protein ntrc differentially influence its activity in response to phosphorylation. | four substitutions at serine residue 160 which increase the activity of the sigma 54-dependent activator protein ntrc in the absence of ntrb have been analysed in detail. mutagenesis of the putative phosphoacceptor site of ntrc and analysis of double mutants indicate that the positive control function of the s160w and s160c mutants is phosphorylation-dependent, whereas the activity of the s160y and s160f mutants is phosphorylation-independent. this was confirmed with two purified mutant proteins ... | 1991 | 1658538 |
| aerobic 2-ketogluconate metabolism of klebsiella pneumoniae nctc 418 grown in chemostat culture. | klebsiella pneumoniae nctc 418 is able to convert 2-ketogluconate intracellularly to 6-phosphogluconate by the combined action of an nadph-dependent 2-ketogluconate reductase and gluconate kinase. synthesis of the former enzyme was maximal under 2-ketogluconate-limited growth conditions. an instantaneous transition to a 2-ketogluconate-excess condition resulted in an acceleration of catabolism of this carbon source, accompanied by complete inhibition of biosynthesis. it is suggested that the cau ... | 1991 | 1659609 |
| beta-lactamase inhibitors: relation between kinetic data and in-vitro synergism studies. | because of the need for control of beta-lactamase-mediated resistance and the recent development of tazobactam, the author has examined the inhibitors of various beta-lactamases for their effectiveness. on the basis of the kinetic data, tazobactam exhibited the highest affinity to various beta-lactamases. a combination with tazobactam was found to enhance the effectiveness of piperacillin against s. aureus producing penicillinase, e. coli producing tem-1 or tem 2 enzymes and class i beta-lactama ... | 1991 | 1661596 |
| effect of an immunomodulating agent, ru 414740, on polymorphonuclear responsiveness after burn injury. | an impairment of polymorphonuclear leukocyte (pmn) functions has been described following burn trauma. it was thus of interest to investigate the effect of ru 41740, an agent known to stimulate these cells, on rat pmn functions after burn injury. in the present study the responsiveness to classical stimuli of pmn from untreated burned rats was approximately 40% lower than healthy control values. in vitro treatment with ru 41740 increased oxidative metabolism of pmns from burned and healthy rats. ... | 1991 | 1661708 |
| [an electron microscopic study of the interaction of bacterial intestinal microflora and rotavirus virions]. | transmission electron microscopy has revealed the capacity of rotaviruses for adsorption on escherichia coli, klebsiella pneumoniae, proteus mirabilis cells and the absence of such capacity with respect to enterococcus faecalis, lactobacillus casei and edwardsiella tarda. different degrees and a varying character of the adsorption of rotaviruses by the representatives of the opportunistic group of bacteria have been established, which may aggravate the course of rotavirus diarrhea due to the agg ... | 1991 | 1661985 |
| evolved neomycin phosphotransferase from an isolate of klebsiella pneumoniae. | a new aminoglycoside resistance gene (apha1-iab) confers high-level resistance to neomycin. the sequence of apha1-iab is closely related to apha1 found in the transposons tn4352, tn903 and tn602. for example, apha1-iab differs from apha1-903 at five nucleotides that result in four amino acid replacements. the enzyme encoded by apha1-iab has a significantly higher turnover number with neomycin, kanamycin and g418 as substrates than does the apha1-903 enzyme. a parsimonious phylogenetic tree sugge ... | 1991 | 1662755 |
| the specificity of bacterial siderophore receptors probed by bioassays. | the ability to utilize siderophores of bacterial and fungal origin has been studied in wild-type and mutant strains of the enterobacterial genera salmonella, escherichia, shigella, moellerella, klebsiella, enterobacter, hafnia, pantoea, ewingella, tatumella, yersinia, and in the non-enterics aeromonas, pseudomonas and aureobacterium. although only a few representative strains were tested, the results show characteristic genus-specific differences in the utilization of hydroxamate and catecholate ... | 1991 | 1663779 |
| phenotypic characterization of quinolone-resistant mutants of enterobacteriaceae selected from wild type, gyra type and multiply-resistant (mara) type strains. | the nctc type strains of escherichia coli, enterobacter cloacae, serratia marcescens and klebsiella pneumoniae were exposed to 3, 5 and 10 x mic of nalidixic acid, enoxacin, ciprofloxacin, pd 117596 and pd 127391. from each strain a mutant with a high mic of quinolones alone (gyra) and a mutant with intermediate resistance to quinolones, some beta-lactams, chloramphenicol and tetracycline (multiply resistant, m-r) were selected on agar containing antibiotics. the gyra mutants required a higher c ... | 1991 | 1663926 |
| the role of the nac protein in the nitrogen regulation of klebsiella aerogenes. | the nac (nitrogen assimilation control) protein from klebsiella aerogenes is a lysr-like regulator for transcription of several operons involved in nitrogen metabolism, and couples the transcription of these sigma 70-dependent operons to regulation by the sigma 54-dependent ntr system. nac activates expression of operons (e.g. histidine utilization, hut), allowing use of poor nitrogen sources, and represses expression of operons (e.g. glutamate dehydrogenase, gdh) allowing assimilation of the pr ... | 1991 | 1664020 |
| in vitro activity and stability against novel beta-lactamases of investigational beta-lactams (cefepime, cefpirome, flomoxef, sce2787 and piperacillin plus tazobactam) in comparison with established compounds (cefotaxime, latamoxef and piperacillin). | the therapeutic perspectives of flomoxef, sce 2787, cefpirome, cefepime, latamoxef, cefotaxime and of piperacillin plus tazobactam were comparatively evaluated by their in vitro activity against 1119 clinical isolates of 83 bacterial species. escherichia coli, klebsiella spp. enterobacter sakazakii, proteus spp. and shigella spp. were about equally susceptible to the cephalosporins (mic90: 0.06 to 0.5 mg/l), while the mic90 for piperacillin plus tazobactam was between 2 and 16 mg/l. enterobacter ... | 1991 | 1664418 |
| na(+)-coupled alternative to h(+)-coupled primary transport systems in bacteria. | protons are the most common coupling ions in bacterial energy conversions. however, while many organisms, such as the alkaliphilic bacilli, employ h(+)-bioenergetics for electron transport phosphorylation, they use na+ as the coupling ion for transport and flagellar movement. the na+ gradient required for these bioenergetic functions is established by the secondary na+/h+ antiporter. in contrast, vibrio alginolyticus and methanogenic bacteria have primary pumps for both h+ and na+. they use the ... | 1991 | 1665692 |
| sinus histiocytosis with massive lymphadenopathy (rosai-dorfman disease) in a patient with elevated epstein-barr virus titers. | sinus histiocytosis with massive lymphadenopathy (shml) is a newly recognized disorder. the etiology of this disease is unknown. an exaggerated response to an offending agent such as the epstein-barr virus or klebsiella bacteria has been postulated. its course is usually benign. cervical adenopathy is seen in 97% of the patients, while 30% of patients have nodal involvement in other sites, and 30% have extranodal involvement. there is a 7% mortality rate that occurs primarily in patients with im ... | 1991 | 1666127 |
| [is cytobacteriological examination of sputum necessary for the diagnosis of bronchopulmonary superinfections in abidjan?]. | about 276 sputums analysed from 1984 to 1989, 61% of them have been positive. beside the usual germs of the oropharynged flora, 115 bacteria susceptible of being pathogenic have been isolated. the kinds which are frequently found are klebsiella = 31 (27%), escherichia coli = 20 (17%), enterobacter = 19 (17%), staphylococcus aureus = 17 (15%) and pseudomonas aeruginosa = 12 (10%). | 1991 | 1666982 |
| in vivo evaluation of nm441, a new thiazeto-quinoline derivative. | nm441 is a lipophilic prodrug of a new thiazeto-quinoline carboxylic acid derivative nm394, and when it is administered orally it is readily absorbed and hydrolyzed to its parent compound. after oral administration of nm441 at a dose of 20 mg/kg to dogs, the peak concentration of nm394 in plasma was 2.39 micrograms/ml, whereas it was 0.63 micrograms/ml for nm394 administered alone. the in vivo activity of nm441 was compared with those of ciprofloxacin, ofloxacin, and enoxacin in mouse protection ... | 1991 | 1667252 |
| [the resistance of the bacterial flora in cancer patients to drug therapy]. | agents etiologically relevant to hospital infection have been studied in an oncologic clinic for the period of 20 years since 1969. pronounced changes in the profile of basic infections have been observed. in 1969, staphylococcus accounted for 46% of cases of infection and e. coli was isolated in 22% whereas in 1987 and 1988, the respective figures for staphylococcus were 18 and 16% only while for e. coli--8.4 and 8.6%, respectively. at the same time, the occurrence of all types of streptococcal ... | 1991 | 1669198 |
| valvular infections in patients with hydrocephalus: preventive aspects. | shunt infections in children have become a serious problem. in order to solve this, we have been using antibiotic therapy with rifampicin (rifampin) for the last 2 years; the dosage is 20 mg/kg per day 1 h before surgery and then for 48 h after the surgical procedure. we have had experience with 203 children operated on between january 1987 and december 1988. the result was a significant decrease in the number of children with shunt infections. in 1980 we reported an incidence of 10%, while by 1 ... | 1990 | 1669249 |
| [substance produced by klebsiella pneumoniae with protects it from the bactericidal effect of normal human serum]. | a klebsiella pneumoniae culture in fouad's chemically defined medium, which was grown from 18 to 24 hours at 37 degrees c was prepared. bacterial cells were eliminated by centrifugation and the supernatant was sterilized by millipore membrane filtration. a protective activity against normal human serum bactericidal effect on k. pneumoniae k-9 and k-13 was ascribed to sterile filtrate. the protective filtrate seems to be a high molecular weight substance. | 1991 | 1670252 |
| [epidemiologic and etiologic study of acute respiratory infections in children under 5 years of age]. | this study also included epidemiologic data to determine the risk factors for the enhancement of these infections. the coagglutination technique for the rapid diagnosis of streptococcus was tried. five hundred and thirty one samples of pharingeal swabs were obtained from children with ari. 74% were younger than one year old. brochopneumonias constituted 66.39% of the cases. in total 357 agents were isolated 35% corresponding to bacteria in pure culture, 23.3% to mixed infections and 11.6% only v ... | 1991 | 1670472 |
| nucleotide sequence and functions of mrk determinants necessary for expression of type 3 fimbriae in klebsiella pneumoniae. | the nucleotide sequence of six genes involved in the expression of type 3 fimbriae of klebsiella pneumoniae was determined. in addition to the genes that encode the fimbrial subunit (mrka) and adhesion (mrkd), the mrkb, mrkc, and mrke genes appear to be involved in assembly of the fimbrial filament and regulation of type 3 fimbrial expression. the mrkf gene product is required to maintain the stability of the fimbrial filament on the cell surface. | 1991 | 1670938 |
| prevalence of nosocomial infections in a saudi arabian teaching hospital. | the rate of nosocomial infections was determined during a 9-month study in a 6-year-old hospital in north saudi arabia. the overall rate of nosocomial infection in the hospital was 2.2%. the rates in the different services varied. the highest were in the special care baby unit (13.5%) and intensive care unit (6%). in the other services it ranged from 1 to 3.5%. the common causal agents of documented infections were escherichia coli (81), pseudomonas aeruginosa (55), staphylococcus aureus (43), k ... | 1991 | 1675653 |
| ceftazidime-resistant klebsiella pneumoniae. | | 1991 | 1675738 |
| characterisation of a pseudomonas aeruginosa twitching motility gene and evidence for a specialised protein export system widespread in eubacteria. | type-4 fimbriae (pili) are associated with a phenomenon known as twitching motility, which appears to be involved with bacterial translocation across solid surfaces. pseudomonas aeruginosa mutants which produce fimbriae, but which have lost the twitching motility function, display altered colony morphology and resistance to fimbrial-specific bacteriophage. we have used phenotypic complementation of such mutants to isolate a region of dna involved in twitching motility. this region was physically ... | 1991 | 1676385 |
| selective decontamination of the gastrointestinal tract as an infection control measure. | an outbreak caused by a klebsiella aerogenes resistant to ceftazidime, cefuroxime, cefotaxime, ampicillin and piperacillin and sensitive to aminoglycosides, imipenem and temocillin occurred in a teaching hospital's busy multi-disciplinary intensive care unit over a 3-month period. four patients had bacteraemia and a further four were colonized. traditional infection control measures failed to eradicate the outbreak. the introduction of a selective gastrointestinal decontamination regimen consist ... | 1991 | 1677652 |
| re-use of enteral feeding tubes--a potential hazard to the patient? a study of the efficiency of a representative range of cleaning and disinfection procedures. | some hospitals and manufacturers are now recommending that patients (particularly those on home enteral feeding) remove and re-insert their tubes on a daily basis. this study was carried out to evaluate the effectiveness of a representative range of currently used cleaning procedures in removing bacteria from the lumina of these tubes. one thousand-ml portions of feed experimentally contaminated with 10(2)-10(3) klebsiella aerogenes ml(-1) were perfused through three types of commonly used polyu ... | 1991 | 1678758 |
| nosocomial infections: cockroaches as possible vectors of drug-resistant klebsiella. | the possibility that hospital cockroaches may act as vectors of drug-resistant klebsiella spp. was investigated during nov 1985 to april 1989, at the all india institute of medical sciences (aiims) hospital. klebsiella spp. (majority klebsiella pneumoniae) were isolated from 28.3% of hospital cockroaches and 28.1% of infected wounds of patients. most of klebsiella isolates from patients (96.3%), and hospital cockroaches (85.9%) showed multiple drug resistance to four or more antimicrobials. simi ... | 1991 | 1678762 |
| epidemiology of enteric bacteria in neonatal units--influence of procedures and patient variables. | among 953 infants in 22 neonatal care units studied, 23% (median value, range 0-78) were found to be faecally colonized with one of 21 distinct nosocomial strains of escherichia coli, klebsiella or enterobacter spp. lower birth weight was associated with such colonization, particularly with nosocomial phenotypes of e. coli. caesarean section followed by prolonged incubator care, and hospital stay, were additional factors associated with colonization by such strains of e. coli. antibiotic therapy ... | 1991 | 1679068 |
| a case-control study of an outbreak of infections caused by klebsiella pneumoniae strains producing ctx-1 (tem-3) beta-lactamase. | in july 1984 klebsiella pneumoniae producing beta-lactamase ctx-1(tem-3) (k. pneumoniae-ctx-1) spread from an intensive care unit (icu) throughout the hospitals of clermont-ferrand, france, and were isolated in four other hospitals of the region. a retrospective case control study was conducted in the icu to characterize the risk factors for nosocomial infection with this organism. the cases were the 74 patients who had had k. pneumoniae-ctx-1 isolated from one or more clinical samples between j ... | 1991 | 1679072 |
| biliary stent associated infection. | | 1991 | 1679079 |
| [the hemagglutinating activity of bacteria in the genus klebsiella and the morphofunctional characteristics of their fimbriae]. | the hemagglutinating activity of 77 klebsiella strains from the international collection, grown in a culture medium prepared on the basis of soy-bean flour enzymatic hydrolysate, was studied. these strains could be divided into four groups according to their capacity for synthesizing different types of hemagglutinins on their surface: 2 strains carried mannose-sensitive hemagglutinins, 18 strains had mannose-resistant k-type hemagglutinins, 48 strains exhibited the signs indicating the presence ... | 1991 | 1679591 |
| involvement of groel in nif gene regulation and nitrogenase assembly. | several approaches were used to study the role of groel, the prototype chaperonin, in the nitrogen fixation (nif) system. an escherichia coli groel mutant transformed with the klebsiella pneumoniae nif gene cluster accumulated very low to nondetectable levels of nitrogenase components compared with the isogenic wild-type strain or the mutant cotransformed with the wild-type groe operon. in k. pneumoniae, overexpression of the e. coli groe operon markedly accelerated the rate of appearance of the ... | 1991 | 1680848 |
| long-acting chloramphenicol versus intravenous ampicillin for treatment of bacterial meningitis. | in most developing countries, bacterial meningitis (bm) is associated with a high case-fatality rate. the search for a simple, convenient, and inexpensive antibiotic treatment remains a priority. in this study, a non-blinded, multicentre, randomised clinical trial of 528 cases of bm was done in two hospitals in mali and niger, between march, 1989, and may, 1990, to see whether a double injection of long-acting chloramphenicol (on admission to hospital and 48 h later) is as effective as a course ... | 1991 | 1681224 |
| selective decontamination of the digestive tract (sdd) in intensive care patients: a critical evaluation of the clinical, bacteriological and epidemiological benefits. | twenty trials (17 controlled and three observational cohort studies) on selective decontamination of the digestive tract (sdd) have been undertaken to date. sdd is defined as a technique which aims to eradicate carriage of disease-causing microorganisms by means of lethal oropharyngeal and faecal antimicrobial concentrations. the sdd concept and the criteria for the choice of the antimicrobials used in the sdd programme are explained. abolition of the carrier state is thought to provide clinical ... | 1991 | 1682365 |
| the product of the nitrogen fixation regulatory gene nfrx of azotobacter vinelandii is functionally and structurally homologous to the uridylyltransferase encoded by glnd in enteric bacteria. | we sequenced the nitrogen fixation regulatory gene nfrx from azotobacter vinelandii, mutations in which cause a nif- phenotype, and found that it encodes a 105-kda protein (nfrx), the n terminus of which is highly homologous to that of the uridylyltransferase-uridylyl-removing enzyme encoded by glnd in escherichia coli. in vivo complementation experiments demonstrate that the glnd and nfrx products are functionally interchangeable. a vinelandii nfrx thus appears to encode a uridylyltransferase-u ... | 1991 | 1683868 |
| chemiluminescence response of human polymorphonuclear leukocytes induced by purified, latex attached klebsiella fimbriae. | type 1 fimbriae (t1f) and type 3 fimbriae (t3f) were isolated from klebsiella species, purified, attached to latex beads and tested for their ability to stimulate human polymorphonuclear leukocyte (pmnl) oxidative activity. the luminol dependent chemiluminescence assay was used to evaluate the response of phagocytes. latex particles coated with type 3 fimbriae (1-t3f) induced a significantly higher chemiluminescence response than those with type 1 fimbriae (1-t1f). opsonization of 1-t1f with poo ... | 1991 | 1684498 |
| fimbrial types among respiratory isolates belonging to the family enterobacteriaceae. | bacterial attachment is believed to be an early step in gram-negative nosocomial pneumonia. the frequency of fimbria-associated adhesins among respiratory pathogens has not been studied in detail. in this study isolates belonging to the family enterobacteriaceae, prospectively obtained from intensive care unit patients who were suspected of having nosocomial pneumonia, were examined for fimbria-associated adhesins. type 3, p, type 1, and other fimbrial phenotypes were identified by specific hema ... | 1991 | 1685495 |
| the surface hydrophobicity and avirulent character of an encapsulated strain of klebsiella pneumoniae. | in order to elucidate how virulence is controlled in encapsulated bacteria, some surface properties of an encapsulated but avirulent strain of klebsiella pneumoniae, strain 277, were examined. although strain 277 was heavily fimbriated, the fimbriae did not demonstrate an avirulent character and were not responsible for the surface hydrophobicity of this strain. the surface hydrophobicity was well correlated with the capacity of the bacteria to associate with polymorphonuclear cells. more bacter ... | 1991 | 1685761 |
| application of two-dimensional nmr spectroscopy in the structural determination of marine natural products. isolation and total structural assignment of 4-deoxyasbestinin diterpenes from the caribbean gorgonian briareum asbestinum. | the structures of four novel asbestinin diterpenes isolated in the toxic extracts of the caribbean gorgonian briareum asbestinum are discussed. the major metabolite 11-acetoxy-4-deoxyasbestinin b was subjected to a total structural assignment through the concerted application of several 2d nmr techniques that included 1h-1h cosy, rct-cosy, rct2 cosy, hc cosy (hmqc), noesy, proton-detected long range heteronuclear chemical shift correlation (hmbc) and 13c-13c chemical shift correlation spectrosco ... | 1991 | 1686898 |
| distribution of alginate gene sequences in the pseudomonas rrna homology group i-azomonas-azotobacter lineage of superfamily b procaryotes. | chromosomal dna from group i pseudomonas species, azotobacter vinelandii, azomonas macrocytogens, xanthomonas campestris, serpens flexibilis, and three enteric bacteria was screened for sequences homologous to four pseudomonas aeruginosa alginate (alg) genes (alga, pmm, algd, and algr1). all the group i pseudomonas species tested (including alginate producers and nonproducers) contained sequences homologous to all the p. aeruginosa alg genes used as probes, with the exception of p. stutzeri, whi ... | 1990 | 1689562 |
| morphological evidence for penetration of anti-o antibody through the capsule of klebsiella pneumoniae. | the role of the capsule in the reaction with anti-o or anti-k serum was examined morphologically by the techniques of freeze-substitution and immunoelectron microscopy in two strains of klebsiella pneumoniae differing in virulence for mice. strain chedid (o1:k2), an encapsulated virulent strain, has a thicker capsule (150 nm) than the encapsulated avirulent strain 277 (o1:k2) (60 nm). two morphologically recognizable domains in the capsule created by the arrangement of the capsular filaments wer ... | 1990 | 1691148 |
| [the etiological structure of acute intestinal infections]. | the share of various etiologic forms of acute intestinal infections, diagnosed by bacteriologic methods, is presented. the share of gastroenterocolitis induced by opportunistic microflora makes up 35.6%, that of dysentery 25.6%, salmonellosis 18.5%; mixed infection (dysentery + salmonellosis) is diagnosed in 7% of cases with acute intestinal infections. the principal representative of opportunistic microflora isolated from patients with acute intestinal infections is the klebsiella genus (40.6%) ... | 1990 | 1692367 |
| sandwich enzyme immunoassays for detection of salmonella typhi. | enzyme immunoassays were developed using monoclonal antibodies raised against somatic (o), flagellar (h) and capsular (vi) antigens of salmonella typhi. the assay based on anti-o monoclonal antibodies could specifically detect s. typhi and soluble lipopolysaccharide (lps) isolated from s. typhi. anti-h moabs detected motile s. typhi and soluble flagellar antigen. monoclonal antibodies against capsular polysaccharide could detect vi-containing s. typhi as well as soluble vi antigen. the three ass ... | 1990 | 1693382 |
| survey of infection in babies at the khoula hospital, oman. | a 12-month prospective study of infection was carried out in the special care baby unit (scbu), khoula hospital, muscat, sultanate of oman. during this period, 8720 babies were born in the hospital and 1265 were admitted to the scbu. altogether, 490 babies were of less than 36 weeks' gestation. a total of 190 babies (160 born in the hospital, 30 born before admission) satisfied the criteria for infection. the most common clinical presentation was pneumonia. there was one outbreak of iatrogenic i ... | 1990 | 1694643 |
| effects of reduced malto-oligosaccharides on the thermal stability of pullulanase from bacillus acidopullulyticus. | we investigated the effects of the reduced malto-oligosaccharides, d-glucitol (g1-oh), maltitol (g2-oh), maltotriitol (g3-oh), maltotetraitol (g4-oh), and maltopentaitol (g5-oh) on the thermal stability of bacillus acidopullulyticus pullulanase (ec 3.2.1.41). the thermal stability depended on the concentration of d-glucitol; after heat treatment for 90 min at 60 degrees in the presence of 0.56, 0.28, 0.14, or 0m g1-oh, the residual activity was 100, 80, 32, and 10% of the control, respectively. ... | 1990 | 1696171 |
| murine monoclonal antibodies to klebsiella pneumoniae protect against lethal endotoxemia and experimental infection with capsulated k. pneumoniae. | to prepare monoclonal antibodies (mabs) directed against the core-lipid a fractions of smooth lipopoly-saccharide (lps) from klebsiella pneumoniae o1:k2, we immunized balb/c mice with the lps-associated proteins plus lps. this preparation exposed the core-lipid a moiety, which is normally hidden in the micellar structure of classical lps preparations. among 10 mabs selected for their reactivity with lps-associated proteins plus lps from k. pneumoniae o1:k2, 6 (3a3, 3c2, 3c4, 7d2, 11c3, and 12b6) ... | 1990 | 1696932 |
| human monoclonal antibodies to pseudomonas aeruginosa type-specific lipopolysaccharides, toxin a and klebsiella capsular polysaccharides. | a number of human monoclonal antibodies (hmab) recognizing type-specific determinants expressed by the lipopolysaccharide (lps) of pseudomonas aeruginosa and by the capsular polysaccharide (cps) of klebsiella were generated for potential treatment of nosocomial infections. the goal is to administer these type-specific hmab prophylactically as a "cocktail" providing broad coverage. lymphoblastoid cell lines (lcl) secreting hmab recognizing p. aeruginosa lps, toxin a or klebsiella cps were obtaine ... | 1990 | 1698165 |