| failure of the microscan walkaway system to detect heteroresistance to carbapenems in a patient with enterobacter aerogenes bacteremia. | we report the failure of the automated microscan walkaway system to detect carbapenem heteroresistance in enterobacter aerogenes. carbapenem resistance has become an increasing concern in recent years, and robust surveillance is required to prevent dissemination of resistant strains. reliance on automated systems may delay the detection of emerging resistance. | 2009 | 19641071 |
| structural flexibility enhances the reactivity of the bioremediator glycerophosphodiesterase by fine-tuning its mechanism of hydrolysis. | the glycerophosphodiesterase from enterobacter aerogenes (gpdq) belongs to the family of binuclear metallohydrolases and has attracted recent attention due to its potential in bioremediation. formation of a catalytically competent binuclear center is promoted by the substrate (hadler et al. j. am. chem. soc. 2008, 130, 14129). using the paramagnetic properties of mn(ii), we estimated the k(d) values for the metal ions in the alpha and beta sites to be 29 and 344 microm, respectively, in the abse ... | 2009 | 19653693 |
| huge symbiotic organs in giant scale insects of the genus drosicha (coccoidea: monophlebidae) harbor flavobacterial and enterobacterial endosymbionts. | giant scale insects (drosicha: coccoldea: monophlebidae) were investigated for their symbiotic organs and bacterial endosymbionts. two types of bacterial 16s rrna gene sequences, flavobacterial and enterobacterial, were consistently detected in d. corpulenta and d. pinicola. the former sequences formed a compact clade in the bacteroidetes, allied to the symbionts of cushion and armored scales. the latter sequences formed a robust clade in the gamma-proteobacteria, allied to enteric bacteria like ... | 2009 | 19663639 |
| antibacterial activity of ethanol extracts of seaweeds against fish bacterial pathogens. | crude extracts were prepared using the solvent ethanol from the seaweeds viz., gracilaria edulis, calorpha peltada and hydroclothres sp. and screening for their antibacterial activity against six bacterial pathogens were also carried out. the test bacterial strains were escherichia coli, enterobacter aerogenes, staphylococcus aureus, pseudomonas aeruginosa, streptococcus faecalis and bacillus cereus. ethanol extract of gracilaria edulis inhibited growth of all the test organisms except bacillus ... | 2009 | 19673167 |
| growth and gaseous emissions of pure and mixed small intestinal bacterial cultures: effects of bile and vancomycin. | simultaneous cultivations in anaerobiosis, aerobiosis and with microaerobic gas mixture were used to clarify the bile (oxgall) effects on the pure and mixed cultures of enterobacterial strains in simulations in portable microbe enrichment unit (pmeu) linked with chempro100i((r)) gas detector. the effects of vancomycin were evaluated in aerobic cultures. growth and metabolic activity of cultures were also followed by measuring sugar consumption, ph alterations, and colony counts on bd chromagar o ... | 2010 | 19682874 |
| magnetic circular dichroism study of a dicobalt(ii) complex with mixed 5- and 6-coordination: a spectroscopic model for dicobalt(ii) hydrolases. | the magnetic circular dichroism (mcd) study of [co(2)(mu-oh)(mu-ph(4)dba)(tmeda)(2)(otf)], in which ph(4)dba is the dinucleating bis(carboxylate) ligand dibenzofuran-4,6-bis(diphenylacetate) and tmeda is n,n,n',n'-tetramethylethylenediamine, is presented. this complex serves as an excellent spectroscopic model for a number of dicobalt(ii) enzymes and proteins that have both the mu-hydroxo, mu-carboxylato bridging and asymmetric 6- and 5-coordination. the low-temperature mcd spectrum of the model ... | 2009 | 19691327 |
| genetic structure and distribution of the colibactin genomic island among members of the family enterobacteriaceae. | a genomic island encoding the biosynthesis and secretion pathway of putative hybrid nonribosomal peptide-polyketide colibactin has been recently described in escherichia coli. colibactin acts as a cyclomodulin and blocks the eukaryotic cell cycle. the origin and prevalence of the colibactin island among enterobacteria are unknown. we therefore screened 1,565 isolates of different genera and species related to the enterobacteriaceae by pcr for the presence of this dna element. the island was dete ... | 2009 | 19720753 |
| use of boronic acid disk tests to detect extended- spectrum beta-lactamases in clinical isolates of kpc carbapenemase-possessing enterobacteriaceae. | we evaluated boronic acid (ba)-based methods for their ability to detect extended-spectrum beta-lactamases (esbls) among clinical isolates of kpc-producing members of the enterobacteriaceae family. a total of 155 isolates of klebsiella pneumoniae (n = 141), escherichia coli (n = 6), enterobacter aerogenes (n = 6), and klebsiella oxytoca (n = 2) genotypically confirmed to be kpc producers were analyzed. as many as 118 isolates harbored esbls (103 harbored shv-type esbls, 13 harbored ctx-m-type es ... | 2009 | 19726597 |
| heparin/heparan sulfate 6-o-sulfatase from flavobacterium heparinum: integrated structural and biochemical investigation of enzyme active site and substrate specificity. | heparin and heparan sulfate glycosaminoglycans (hsgags) comprise a chemically heterogeneous class of sulfated polysaccharides. the development of structure-activity relationships for this class of polysaccharides requires the identification and characterization of degrading enzymes with defined substrate specificity and enzymatic activity. toward this end, we report here the molecular cloning and extensive structure-function analysis of a 6-o-sulfatase from the gram-negative bacterium flavobacte ... | 2009 | 19726671 |
| heparin/heparan sulfate n-sulfamidase from flavobacterium heparinum: structural and biochemical investigation of catalytic nitrogen-sulfur bond cleavage. | sulfated polysaccharides such as heparin and heparan sulfate glycosaminoglycans (hsgags) are chemically and structurally heterogeneous biopolymers that that function as key regulators of numerous biological functions. the elucidation of hsgag fine structure is fundamental to understanding their functional diversity, and this is facilitated by the use of select degrading enzymes of defined substrate specificity. our previous studies have reported the cloning, characterization, recombinant express ... | 2009 | 19726673 |
| chemical composition, antifungal and antibacterial activity of the essential oil of chamaecyparis nootkatensis from spain. | the chemical composition of the oil of chamaecyparis nootkatensis (d. don) spach. has been analysed by gas chromatography (gc) and gas chromatography coupled to mass spectrometry (gc-ms). a total of 34 compounds were identified representing more than 90% of the total oil. the oil was richer in monoterpenes than in sesquiterpenes, the major constituents being limonene (53.2%), 6-3-carene (21.0%) and a-pinene (12.2%). the antibacterial and antifungal activities of the oil were also tested against ... | 2009 | 19731613 |
| extended-spectrum beta-lactamases among enterobacteriaceae isolated in a public hospital in brazil. | extended-spectrum beta-lactamases (esbl) in enterobacteria are recognized worldwide as a great hospital problem. in this study, 127 esbl-producing enterobacteriaceae isolated in one year from inpatients and outpatients at a public teaching hospital at são paulo, brazil, were submitted to analysis by pcr with specific primers for bla shv, bla tem and bla ctx-m genes. from the 127 isolates, 96 (75.6%) klebsiella pneumoniae, 12 (9.3%) escherichia coli, 8 (6.2%) morganella morganii, 3 (2.3%) proteus ... | 2009 | 19739000 |
| biological properties of the chilean native moss sphagnum magellanicum. | an ethanol extract prepared from the gametophyte chilean native moss sphagnum magellanicum was dried out, weighed and dissolved in distilled water. this extract was then assayed for its antibacterial activity against the g(-) bacteria azotobacter vinelandii, erwinia carotovora subsp. carotovora, enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, salmonella typhi, vibrio cholerae, and the g(+) bacteria staphylococcus aureus subsp. aureus, and streptococcus type beta. the growth of ... | 2009 | 19746269 |
| a genome-scale metabolic model of arabidopsis and some of its properties. | we describe the construction and analysis of a genome-scale metabolic model of arabidopsis (arabidopsis thaliana) primarily derived from the annotations in the aracyc database. we used techniques based on linear programming to demonstrate the following: (1) that the model is capable of producing biomass components (amino acids, nucleotides, lipid, starch, and cellulose) in the proportions observed experimentally in a heterotrophic suspension culture; (2) that approximately only 15% of the availa ... | 2009 | 19755544 |
| isolation of salmonella and shigella from fish harvested from the winam gulf of lake victoria, kenya. | human infections caused by pathogens transmitted from fish are quite common. the aim of this study was to isolate enteric pathogenic bacteria from fish that might be transmitted to humans after the handling or consumption of such fish. | 2009 | 19755738 |
| [isolation and identification of degradation bacteria enterobacter aerogenes for pyrethriods pesticide residues and its degradation characteristics]. | by incubation experiment, the bacterial strain labeled as m6r9 was isolated from the tame sludge in water course of pesticide factory of hangzhou, and was identified as enterobacter aerogenes, which had highly efficient degradation for bifenthrin, fenpropathrin and cypermethrin. by investigating the physiological characteristics of the strain, the results show that the bacterium is a gram-negative aerobe bacilli, size is (0.8-1.9) microm x (0.5-1.0) microm, and is capable of utilizing bifenthrin ... | 2009 | 19799315 |
| role of intracellular polysaccharide in persistence of streptococcus mutans. | intracellular polysaccharide (ips) is accumulated by streptococcus mutans when the bacteria are grown in excess sugar and can contribute toward the cariogenicity of s. mutans. here we show that inactivation of the glga gene (smu1536), encoding a putative glycogen synthase, prevented accumulation of ips. ips is important for the persistence of s. mutans grown in batch culture with excess glucose and then starved of glucose. the ips was largely used up within 1 day of glucose starvation, and yet s ... | 2009 | 19801415 |
| antibacterial activity of ceftazidime (gr 20263) against routine hospital isolates and highly resistant strains of enterobacteriaceae and pseudomonas aeruginosa. | ceftazidime was found to be highly active against routine hospital isolates of strains of enterobacteriaceae such as escherichia coli, klebsiella aerogenes, enterobacter cloacae and proteus mirabilis, with the mean mics varying between 0.11 and 0.75 mg/l. it had marked activity against pseudomonas aeruginosa with a mean mic of 1.8 mg/l which was comparable to tobramycin (11 mg/l) and superior to other aminoglycosides, penicillins and cefotaxime. although ceftazidime was considerably more active ... | 1981 | 19802975 |
| ceftazidime, a pseudomonas-active cephalosporin: in-vitro antimicrobial activity evaluation including recommendations for disc diffusion susceptibility tests. | the in-vitro qualities of ceftazidime, as compared to other beta-lactams and three other antimicrobials (amikacin, gentamicin and chloramphenicol), were evaluated in a multilaboratory, multiphasic study in the united states. a total of 12,986 recent clinical isolates were tested by reference dilution methods in six medical centres over 45-60 days. ceftazidime was superior to the comparison cephalosporin (cefamandole) and comparable in spectrum and activity to gentamicin against enterobacteriacea ... | 1981 | 19802985 |
| synthesis of n-tetra-o-acetyl-beta-d-glucopyranosyl-n'-(4',6'-diarylpyrimidin-2'-yl)thioureas. | some 2-amino-4,6-diarylpyrimidines 2 have been prepared from substituted benzylideneacetophenones and guanidine hydrochloride in the presence of alkali by conventional heating in alcoholic medium and microwave heating in solvent-free conditions. n-(2,3,4,6-tetra-o-acetyl-beta-d-glucopyranosyl)-n'-(4',6'-diarylpyrimidin-2'-yl)thioureas 4 have been synthesized by reaction of per-o-acetylated glucopyranosyl isothiocyanate 1 and substituted 2-amino-4,6-diarylpyrimidines 2. two different methods have ... | 2009 | 19804880 |
| chemical composition and antimicrobial activity of the extracts of kefe cumin (laser trilobum l.) fruits from different regions. | structural properties (using gas chromatographic analyses: gas chromatography and gas chromatography-mass spectrometry) of some compounds (pure chemicals, essential oils, extracts, etc.) and systematic investigation of antimicrobial activities (with agar dilution and agar diffusion disc methods) were determined in the fruits of kefe cumin (laser trilobum l.) obtained from different regions (germany, india, and turkey). the main components were established as limonene (41.03-72.24%) and perillald ... | 2009 | 19817640 |
| tonb-dependent transporters and their occurrence in cyanobacteria. | different iron transport systems evolved in gram-negative bacteria during evolution. most of the transport systems depend on outer membrane localized tonb-dependent transporters (tbdts), a periplasma-facing tonb protein and a plasma membrane localized machinery (exbbd). so far, iron chelators (siderophores), oligosaccharides and polypeptides have been identified as substrates of tbdts. for iron transport, three uptake systems are defined: the lactoferrin/transferrin binding proteins, the porphyr ... | 2009 | 19821963 |
| alteration of hydrogen metabolism of ldh-deleted enterobacter aerogenes by overexpression of nad+-dependent formate dehydrogenase. | the nad+-dependent formate dehydrogenase fdh1 gene (fdh1), cloned from candida boidinii, was expressed in the ldh-deleted mutant of enterobacter aerogenes iam1183 strain. the plasmid of pcom10 driven by the palkb promoter was used to construct the fdh1 expression system and thus introduce a new dihydronicotinamide adenine dinucleotide (nadh) regeneration pathway from formate in the ldh-deleted mutant. the knockout of nadh-consuming lactate pathway affected the whole cellular metabolism, and the ... | 2010 | 19830418 |
| the in-vitro antimicrobial activity of some medicinal plants against beta-lactam-resistant bacteria. | in effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria. | 2009 | 19858568 |
| assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance. | to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health. | 2009 | 19880150 |
| the bioremediator glycerophosphodiesterase employs a non-processive mechanism for hydrolysis. | glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a binuclear metallohydrolase that catalyzes the breakdown of a broad range of phosphate ester substrates, and it is of interest for its potential application in the destruction of organophosphate nerve agents and pesticides. the reaction mechanism of gpdq has been proposed to involve a nucleophilic attack by a terminally bound hydroxide molecule. the hydroxide species bridging the two metal ions is suggested to activate the nucleophi ... | 2010 | 19923005 |
| relationship between structure and antibacterial activity of lipophilic n-acyldiamines. | we report in this work the preparation and antibacterial evaluation of a series of n-monoacylated diamines against six gram-positive and 11 gram-negative bacteria. the results obtained showed the existence of relationship between lipophilicity and antibacterial activity of the tested compounds. the best results were obtained against gram-positive bacteria for compounds having a 10-12 carbons alkyl chain. compound 4e was the most active against microccus lentus (mic=2 microg/ml), staphylococcus a ... | 2010 | 19942397 |
| clinical characteristics and predictors of mortality in patients with enterobacter aerogenes bacteremia. | enterobacter aerogenes is increasingly encountered in nosocomial infections. this study aimed to clarify the clinical characteristics and to identify the predictors of mortality in patients with e. aerogenes bacteremia. | 2009 | 19949757 |
| international spread and persistence of tem-24 is caused by the confluence of highly penetrating enterobacteriaceae clones and an inca/c2 plasmid containing tn1696::tn1 and is5075-tn21. | tem-24 remains one of the most widespread tem-type extended-spectrum beta-lactamases (esbls) among enterobacteriaceae. to analyze the reasons influencing its spread and persistence, a multilevel population genetics study was carried out on 28 representative tem-24 producers from belgium, france, portugal, and spain (13 enterobacter aerogenes isolates, 6 escherichia coli isolates, 6 klebsiella pneumoniae isolates, 2 proteus mirabilis isolates, and 1 klebsiella oxytoca isolate, from 1998 to 2004). ... | 2010 | 19995930 |
| significance of infection markers and microbiological findings during tissue processing of cryopreserved arterial homografts for the early postoperative course. | to evaluate homograft implantation for the urgent treatment of vascular infections on the basis of the course of infection using microbiological findings in perioperatively obtained specimens and during homograft processing. | 2009 | 19998257 |
| bacteriological evaluation of pre-cut fruits sold in kano metropolis, kano state, nigeria. | one hundred and fifty (150) pre-cut fruit samples comprising of pineapples (50), paw-paw (50) and watermelon (50) at the point of stand retail outlets were tested by standard bacteriological methods to determine bacterial contamination of the fruits. out of these 150 examined 136 (90.67%) were contaminated with bacteria. the bacterial distribution were; escherichia coli 69 (46.00%), staphylococcus aureus 29 (19.33%), salmonella species 13 (8.67%), proteus species 18 (12.00%), enterobacter aeroge ... | 2009 | 20000065 |
| reconstruction of the violacein biosynthetic pathway from duganella sp. b2 in different heterologous hosts. | violacein is a bacteria-originated indolocarbazole pigment with potential applications due to its various bioactivities such as anti-tumor, antiviral, and antifungal activities. however, stable mass production of this pigment is difficult due to its low productivities and the instability of wild-type violacein-producing strains. in order to establish a stable and efficient production system for violacein, the violacein synthesis pathway from a new species of duganella sp. b2 was reconstructed in ... | 2010 | 20012278 |
| gas chromatographic-mass spectrometric analysis of volatiles obtained by four different techniques from salvia rosifolia sm., and evaluation for biological activity. | four different isolation techniques, conventional hydrodistillation (hd), microwave-assisted hydrodistillation (mwhd), microdistillation (md) and micro-steam distillation-solid-phase microextraction (msd-spme), have been used to analyze the volatile constituents from the aerial parts of salvia rosifolia sm. by gas chromatography and gas chromatography coupled to mass spectrometry. hd and mwhd techniques produced quantitatively (yield, 0.39% and 0.40%) and qualitatively (aromatic profile) similar ... | 2010 | 20015509 |
| transfer of a phage t4 gene into enterobacteriaceae, determined at the single-cell level. | the transfer range of phage genes was investigated at the single-cell level by using an in situ dna amplification technique. after absorption of phages, a phage t4 gene was maintained in the genomes of non-plaque-forming bacteria at frequencies of 10(-2) gene copies per cell. the gene transfer decreased the mutation frequencies in nonhost recipients. | 2010 | 20023087 |
| method to detect only viable cells in microbial ecology. | propidium monoazide can limit the analysis of microbial communities derived from genetic fingerprints to viable cells with intact cell membranes. however, pma treatment cannot completely suppress polymerase chain reaction (pcr) amplification when the targeted gene is too short. pma treatment in combination with two-step nested pcr was designed to overcome this problem. four experiments were performed to determine the limitation of pma treatment and to evaluate the suitability of the method by ap ... | 2010 | 20024544 |
| biogenic silver for disinfection of water contaminated with viruses. | the presence of enteric viruses in drinking water is a potential health risk. growing interest has arisen in nanometals for water disinfection, in particular the use of silver-based nanotechnology. in this study, lactobacillus fermentum served as a reducing agent and bacterial carrier matrix for zerovalent silver nanoparticles, referred to as biogenic ag(0). the antiviral action of biogenic ag(0) was examined in water spiked with an enterobacter aerogenes-infecting bacteriophage (uz1). addition ... | 2010 | 20038697 |
| metabolism of (-)-epigallocatechin gallate by rat intestinal flora. | anaerobic metabolism of (-)-epigallocatechin gallate (egcg) by rat intestinal bacteria was investigated in vitro. first, intestinal bacteria which are capable of hydrolyzing egcg to (-)-epigallocatechin (egc) and gallic acid (2) were screened with 169 strains of enteric bacteria. as a result, enterobacter aerogenes, raoultella planticola, klebsiella pneumoniae susp. pneumoniae, and bifidobacterium longum subsp. infantis were found to hydrolyze egcg. subsequent steps of egcg metabolism are degrad ... | 2010 | 20043675 |
| trends in antimicrobial susceptibility of gram-negative bacteria isolated from blood in jakarta from 2002 to 2008. | this study examined the susceptibility of gram-negative bacteria in the bloodstream to antimicrobials with the aim of providing information relevant to the guidance of therapy. | 2009 | 20061679 |
| infection of brindley sacral anterior root stimulator by pseudomonas aeruginosa requiring removal of the implant: long-term deleterious effects on bowel and urinary bladder function in a spinal cord injury patient with tetraplegia: a case report. | we report infection of brindley sacral anterior root stimulator in a spinal cord injury patient, who ultimately required removal of the implant. the consequences of failed implantation were severe constipation, and loss of reflex penile erection and bladder emptying. | 2009 | 20062610 |
| characterization of esbl (shv-12) producing clinical isolate of enterobacter aerogenes from a tertiary care hospital in nigeria. | we studied the beta-lactamases of an e. aerogenes isolate recovered from the blood of a two-year-old patient. the isolate demonstrated a disk-diffusion phenotype typical for an ampc-esbl co-producer. | 2010 | 20067633 |
| comparison of the clinical and microbiologic characteristics of patients with enterobacter cloacae and enterobacter aerogenes bacteremia: a prospective observation study. | we compared the characteristics and outcomes of 172 enterobacter cloacae bacteremia and 67 enterobacter aerogenes bacteremia (eab) cases. antimicrobial resistance rates to e. cloacae were higher than those to e. aerogenes. however, eab more frequently presented as septic shock and was associated with poorer outcomes. | 2010 | 20071128 |
| [investigation of antibacterial activity of sertralin]. | sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ... | 2009 | 20084919 |
| plasmid profile of esbl producing gram-negative bacteria and correlation with susceptibility to beta-lactam drugs. | members of family enterobacteriaceae can acquire resistance to extended spectrum beta lactams by a number of mechanisms; most important being the plasmid encoded extended spectrum beta lactamase (esbl) and ampc beta lactamase. this study has been designed to look for the presence of plasmids and their correlation with drug resistance. | 2010 | 20090229 |
| antibacterial activity of artemisia nilagirica leaf extracts against clinical and phytopathogenic bacteria. | the six organic solvent extracts of artemisia nilagirica were screened for the potential antimicrobial activity against phytopathogens and clinically important standard reference bacterial strains. | 2010 | 20109237 |
| free-living amoebae isolated from water-hyacinth root (eichhornia crassipes). | free-living amoebae are widely distributed in aquatic environments and their hygienic, medical and ecological relationships to man are increasingly important. the purpose of this study was to isolate free-living amoebae from water-hyacinth root (eichhornia crassipes) and the water of an urban lake in mexico city. five grams of wet root were seeded on non-nutritive agar with enterobacter aerogenes (nne). water samples were concentrated by centrifugation at 1200g for 15min and the pellet was seede ... | 2010 | 20117108 |
| patterns of antimicrobial therapy in severe nosocomial infections: empiric choices, proportion of appropriate therapy, and adaptation rates--a multicentre, observational survey in critically ill patients. | this prospective, observational multicentre (n=24) study investigated relationships between antimicrobial choices and rates of empiric appropriate or adequate therapy, and subsequent adaptation of therapy in 171 icu patients with severe nosocomial infections. appropriate antibiotic therapy was defined as in vitro susceptibility of the causative pathogen and clinical response to the agent administered. in non-microbiologically documented infections, therapy was considered adequate in the case of ... | 2010 | 20122817 |
| efflux pumps are involved in the defense of gram-negative bacteria against the natural products isobavachalcone and diospyrone. | the activities of two naturally occurring compounds, isobavachalcone and diospyrone, against documented strains and multidrug-resistant (mdr) gram-negative bacterial isolates were evaluated. the results indicated that the two compounds exhibited intrinsic antibacterial activity against several gram-negative bacteria, and their activities were significantly improved in the presence of an efflux pump inhibitor (mic values decreased to below 10 microg/ml). in addition, the activities of isobavachal ... | 2010 | 20160051 |
| evaluation of phytochemical and antibacterial potential of helicteres isora l. fruits against enteric bacterial pathogens. | antibacterial activities of aqueous, acetone, ethanol and methanol extracts of fruits of helicteres isora (mororphali) were studied. the fruit aqueous extracts of h. isora showed prominent antibacterial activities against e. coli, staphylococcus epidermidis, salmonella typhimurium and proteus vulgaris; moderate activity against enterobacter aerogenes, staphylococcus aureus, salmonella typhi and least activity against pseudomonas aeruginosa. the aqueous extract showed maximal, the ethanol and met ... | 2008 | 20161950 |
| electronic structure analysis of the dinuclear metal center in the bioremediator glycerophosphodiesterase (gpdq) from enterobacter aerogenes. | the glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a promiscuous, dinuclear metallohydrolase that has potential application in the remediation of organophosphate nerve agents and pesticides. gpdq employs an unusual reaction mechanism in which the enzyme is predominantly mononuclear in the resting state, and substrate binding induces the formation of the catalytically competent dinuclear center (hadler et al. j. am. chem. soc. 2008, 130, 14129). reactivity is further modulated by ... | 2010 | 20163105 |
| synthesis and antibacterial assessment of n-[4-(4-substituted phenyl)-1,3-thiazol-2-yl]-1,3,5-triazin-2-amine. | in a wide search program towards new and efficient antibacterial agents, we assessed the extent to which physicochemical properties can be exploited to promote antibacterial activity associated with a series of substituted s-triazine. the synthesized compounds (1a-12b) were subsequently screened for their in vitro antibacterial activity against three gram positive (bacillus subtilis, bacillus cereus, staphylococcus aureus) and three gram-negative microorganism (salmonella typhi, escherichia coli ... | 2009 | 20177467 |
| in vitro glucuronidation of 2,2-bis(bromomethyl)-1,3-propanediol by microsomes and hepatocytes from rats and humans. | 2,2-bis(bromomethyl)-1,3-propanediol (bmp) is a brominated flame retardant used in unsaturated polyester resins. in a 2-year bioassay bmp was shown to be a multisite carcinogen in rats and mice. because glucuronidation is the key metabolic transformation of bmp by rats, in this study the in vitro hepatic glucuronidation of bmp was compared across several species. in addition, the glucuronidation activities of human intestinal microsomes and specific human hepatic udp-glucuronosyltransferase (ugt ... | 2010 | 20200232 |
| characterization of the klebsiella aerogenes urease accessory protein ured in fusion with the maltose binding protein. | assembly of the klebsiella aerogenes urease metallocenter requires four accessory proteins, ured, uree, uref, and ureg, to effectively deliver and incorporate two ni2+ ions into the nascent active site of the urease apoprotein (ureabc). each accessory protein has been purified and characterized with the exception of ured due to its insolubility when it is overproduced in recombinant cells. in this study, a translational fusion was made between the maltose binding protein (mbp) and ured, with the ... | 2010 | 20207756 |
| analysis of multiple metabolites of tocopherols and tocotrienols in mice and humans. | tocopherols and tocotrienols, collectively known as vitamin e, are essential antioxidant nutrients. the biological fates and metabolite profiles of the different forms are not clearly understood. the objective of this study is to simultaneously analyze the metabolites of different tocopherols and tocotrienols in mouse and human samples. using hplc/electrochemical detection and mass spectrometry, 18 tocopherol-derived and 24 tocotrienol-derived side-chain degradation metabolites were identified i ... | 2010 | 20222730 |
| meningitis caused by friedländer's bacillus or aerobacter aerogenes; report of two cases with autopsies. | | 1947 | 20256390 |
| aerobacter aerogenes meningitis. | | 1947 | 20260141 |
| pathological fracture of the right distal radius caused by enterobacter aerogenes osteomyelitis in an adult. | a pathological fracture is a break in a diseased bone caused by weakening of the bone structure by a pathological process with no identifiable trauma. acute bacterial osteomyelitis that results in pathological fractures in the extremities is rare in adults. to our knowledge, we report the first case of enterobacter aerogenes osteomyelitis of the right distal radius, complicated with a pathological fracture, in a 79-year-old man with diabetes, which was diagnosed by radiological, microbiological, ... | 2010 | 20335785 |
| antibacterial activity of leaves of cadaba trifoliata (roxb.) wt. & arn. | antibacterial activity of aqueous and ethanol leaf extracts of cadaba trifoliata was evaluated by cup plate method against bacterial strains such as staphylococcus aureus, bacillus subtilis, acinetobacter, enterobacter aerogenes, erwinia and escherichia coli. the ethanol extract of the leaves demonstrated a high degree of activity against all the tested bacterial strains except erwinia and acinetobacter, whereas the aqueous extract of the leaves showed moderate activity against e. coli, b. subti ... | 2009 | 20336203 |
| metallo-β-lactamases among enterobacteriaceae from routine samples in an italian tertiary-care hospital and long-term care facilities during 2008. | the emergence of metallo-β-lactamase (mbl)-producing enterobacteriaceae is a serious public health concern. producers have been repeatedly isolated from patients and long-term care facility (ltcf) residents around bolzano, and we sought to assess their prevalence and clinical impact. all routine enterobacteriaceae isolates from a bolzano tertiary-care hospital and associated long-term care facilities in 2008 (n = 5500) were screened for mbls, with case details reviewed for the source patients. i ... | 2011 | 20345467 |
| evaluation of etest to determine tigecycline mics for enterobacter species. | | 2010 | 20350941 |
| development of molecular-based methods for determination of high histamine producing bacteria in fish. | histamine (or scombroid) fish poisoning is a significant cause of food borne disease in the united states. in this study, we describe the development of a molecular-based technique which uses digoxigenin (dig) labeled dna probes for the detection of gram negative bacteria producing high amounts of histamine (>1000 ppm). a cocktail of pcr amplification fragments corresponding to a 709 bp fragment of the histidine decarboxylase (hdc) gene of four high producing bacteria (morganella morganii, enter ... | 2010 | 20392504 |
| evaluation of efflux activity of bacteria by a semi-automated fluorometric system. | a semi-automated method that uses the common efflux pump (ep) substrate ethidium bromide (eb) is described for the assessment of ep systems of bacteria. the method employs the rotor-gene(tm) 3000 thermocycler (corbett research) for the real-time assessment of accumulation and efflux of eb in phosphate-buffered solution (pbs) under varying physiological conditions, such as temperature, ph, presence and absence of the energy source, and presence of efflux pumps inhibitors (epis). the method is suf ... | 2010 | 20401593 |
| a highly sensitive detection platform based on surface-enhanced raman scattering for escherichia coli enumeration. | a very sensitive and highly specific heterogeneous immunoassay system, based on surface-enhanced raman scattering (sers) and gold nanoparticles, was developed for the detection of bacteria and other pathogens. two different types of gold nanoparticles (citrate-stabilized gold nanosphere and hexadecyltrimethylammonium bromide (ctab)-stabilized gold nanorod particles) were examined and this immunoassay was applied for the detection of escherichia coli. raman labels were constructed by using these ... | 2010 | 20401720 |
| [bacterial meningitis due to gram-negative bacilli in adults]. | gram-negative bacilli are causative agents of bacterial meningitis in the neonatal period but rarely cause meningitis in adults without risk factors. | 2010 | 20414871 |
| the role of bioactive substances in controlling foodborne pathogens derived from metasequoia glyptostroboides miki ex hu. | in an attempt to isolate bioactive substances, ethyl acetate cone extract of metasequoia glyptostroboides was subjected to a column chromatographic analysis that resulted in isolation of an abietane type diterpenoid, taxoquinone. its structure was elucidated by spectroscopic means. in further, taxoquinone showed potential antibacterial effect as diameters of zones of inhibition against foodborne pathogenic bacteria such as listeria monocytogenes atcc 19166, salmonella typhimurium kctc 2515, salm ... | 2010 | 20435080 |
| screening of antibacterial potentials of some medicinal plants from melghat forest in india. | cyperus rotundus, caesalpinia bonducella, tinospora cordifolia, gardenia gummifera, ailanthus excelsa, acacia arabica, embelia ribes and ventilago maderspatana from melghat forest were screened for their antibacterial potential against escherichia coli, staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris, salmonella typhi, shigella flexneri, salmonella paratyphi, salmonella typhimurium, pseudomonas aeruginosa, enterobacter aerogenes by disc diffusion method. out of these medicinal pla ... | 2009 | 20448847 |
| the filter-feeding ciliates colpidium striatum and tetrahymena pyriformis display selective feeding behaviours in the presence of mixed, equally-sized, bacterial prey. | this study examined whether two ciliates could discriminate between equally-sized bacterial prey in mixture and if so, how selectivity might benefit the ciliate population. live klebsiella aerogenes, k. ozaenae and escherichia coli, expressing different coloured fluorescent proteins, were cultured in such a way as to provide populations containing equally-sized cells (to prevent size-selective grazing taking place) and these prey were fed to each ciliate in 50:50 mixtures. colpidium striatum sel ... | 2010 | 20471910 |
| quinazoline derivatives are efficient chemosensitizers of antibiotic activity in enterobacter aerogenes, klebsiella pneumoniae and pseudomonas aeruginosa resistant strains. | amongst the three series of quinazoline derivatives synthesised and studied in this work, some molecules increase the antibiotic susceptibility of gram-negative bacteria presenting multidrug-resistant phenotypes. n-alkyl compounds induced an increase in the activity of chloramphenicol, nalidixic acid and sparfloxacin, which are substrates of the acrab-tolc and mexab-oprm efflux pumps in clinical isolates. these molecules are able to increase the intracellular concentration of chloramphenicol in ... | 2010 | 20494558 |
| determination of antibacterial and antioxidant potential of some medicinal plants from saurashtra region, india. | many plants used in saurashtra folk medicine have been reported to exhibit high antibacterial and antioxidant activities. in the present study, some parts of five plants, guazuma ulmifolia l., manilkara zapota l., melia azedarach l., syzygium cumini l. and wrightia tomentosa r.& s., were evaluated for their antibacterial activity, total phenol content, flavonoid content, 2,2-diphenyl-1-picrylhydrazyl free radical scavenging activity and phytochemical analysis, using successive extraction by cold ... | 2009 | 20502546 |
| isolation of bacteriocin-like substances producing bacteria from finished cattle-manure compost and activity evaluation against some food-borne pathogenic and spoilage bacteria. | a finished compost sample was examined for bacteriocin-like substance production against five pathogenic bacteria: salmonella typhimurium ef 85-9, escherichia coli o157:h7 atcc 43888, enterococcus faecalis jcm 8726, staphylococcus aureus jcm 2151, and yersinia enterocolitica jcm 7577. at the preliminary detection of bacterial strains exhibiting antimicrobial activity from the compost sample, thirteen strains could be isolated. screening of the inhibitory activity was done using agar-well diffusi ... | 2010 | 20513963 |
| mutagenesis of klebsiella aerogenes ureg to probe nickel binding and interactions with other urease-related proteins. | ureg is a gtpase required for assembly of the nickel-containing active site of urease. herein, a strep-tagged klebsiella aerogenes ureg (ureg(str)) and selected site-directed variants of ureg(str) were constructed for studying the in vivo effects on urease activation in recombinant escherichia coli cells, characterizing properties of the purified proteins, and analysis of in vivo and in vitro protein-protein interactions. whereas the strep tag had no effect on ureg's ability to activate urease, ... | 2010 | 20533838 |
| coliform accumulation in amphibalanus amphitrite (darwin, 1854) (cirripedia) and its use as an organic pollution bioindicator in the estuarine area of recife, pernambuco, brazil. | samples of water and barnacles amphibalanus amphitrite were collected from recife, brazil, to assess if it accumulates total (tc) and thermotolerant coliforms (ttc) related with sewage pollution. the most probable number (mpn) values and the standard procedures for examination of shellfish were used. comparatively with the water samples, the highest coliform values came from the barnacles, with tc values ranging from < 3.0 x 10(3) to > 2.4 x 10(6) mpn.g-1, and ttc ranging from > 2.4 x 10(3) to 2 ... | 2010 | 20549061 |
| bacterial coinfection and antimicrobial resistance in patients with paranasal sinus fungus balls. | the aim of this study was to investigate the incidence of bacterial coinfection and antimicrobial resistance in cases of fungus balls of the paranasal sinuses. | 2010 | 20583740 |
| development of enterobacter aerogenes fuel cells: from in situ biohydrogen oxidization to direct electroactive biofilm. | this study described an enterobacter aerogenes-catalyzed microbial fuel cell (mfc) with a carbon-based anode that exhibited a maximum power density of 2.51 w/m(3) in the absence of artificial electron mediators. the mfc was started up rapidly, within hours, and the current generation in the early stage was demonstrated to result from in situ oxidation of biohydrogen produced by e. aerogenes during glucose fermentation. over periodic replacement of substrate, both planktonic biomass in the cultur ... | 2011 | 20598528 |
| structure of rv1848 (urea), the mycobacterium tuberculosis urease gamma subunit. | the crystal structure of the urease gamma subunit (urea) from mycobacterium tuberculosis, rv1848, has been determined at 1.8 a resolution. the asymmetric unit contains three copies of rv1848 arranged into a homotrimer that is similar to the urea trimer in the structure of urease from klebsiella aerogenes. small-angle x-ray scattering experiments indicate that the rv1848 protein also forms trimers in solution. the observed homotrimer and the organization of urease genes within the m. tuberculosis ... | 2010 | 20606272 |
| in vitro antibacterial activity of ibuprofen and acetaminophen. | ibuprofen and acetaminophen are common chemical agents that have anti-inflammatory, antipyretic, and analgesic activity. | 2010 | 20606962 |
| isolation, structure determination and antibacterial activities of succinamide conjugate diacid from acinetobacter sp. bj-l. | strain bj-l was isolated from the a water sample taken from xiao yue river in beijing and identified as acinetobacter sp. bj-l based on the study of its morphology, physiology, biochemistry and 16s rrna gene sequence. a new antimicrobial substance was produced after the strain was incubated in potato extract medium at 15°c for 72 h. the antimicrobial substance was sequentially purified by reduced pressure condensation, etoac extract, and silica gel column chromatography. the structure of the ant ... | 2011 | 20630726 |
| a nac for regulating metabolism: the nitrogen assimilation control protein (nac) from klebsiella pneumoniae. | the nitrogen assimilation control protein (nac) is a lysr-type transcriptional regulator (lttr) that is made under conditions of nitrogen-limited growth. nac's synthesis is entirely dependent on phosphorylated ntrc from the two-component ntr system and requires the unusual sigma factor σ54 for transcription of the nac gene. nac activates the transcription of σ70-dependent genes whose products provide the cell with ammonia or glutamate. nac represses genes whose products use ammonia and also repr ... | 2010 | 20675498 |
| [cloning of enterobacter aerogenes fh1a gene and overexpression of hydrogen production]. | we amplified and overexpressed the fhl activator (fh1a) in e. aerogenes atcc13408 to enhance hydrogen production. | 2010 | 20687337 |
| physiological characterisation of the efflux pump system of antibiotic-susceptible and multidrug-resistant enterobacter aerogenes. | enterobacter aerogenes predominates amongst enterobacteriaceae species that are increasingly reported as producers of extended-spectrum beta-lactamases. although this mechanism of resistance to beta-lactams is important, other mechanisms bestowing a multidrug-resistant (mdr) phenotype in this species are now well documented. amongst these mechanisms is the overexpression of efflux pumps that extrude structurally unrelated antibiotics prior to their reaching their targets. interestingly, although ... | 2010 | 20688487 |
| identification and characterization of a phospholipase a2 from the venom of the saw-scaled viper: novel bactericidal and membrane damaging activities. | phospholipase a(2) (pla(2)), a common toxic component of snake venom, has been implicated in various pharmacological effects. in this study, a basic myotoxic pla(2), named ectx-i was isolated from echis carinatus snake venom by using gel filtration on superdex g-75, and reverse phase hplc on c18 and c8 sepharose columns. pla(2), ectx-i was 13,861.72 molecular weight as estimated by maldi-tof (15 kd by sds-page), and consisted of 121 amino acid residues cross-linked by seven disulfide bonds. the ... | 2010 | 20723574 |
| synergistic interaction of phenylpropanoids with antibiotics against bacteria. | phenylpropanoids constitute a large part of our daily diet and there is a possibility that they might interact with synthetic drugs. the present work was aimed at studying the interaction of seven phenylpropanoids (cinnamic, p-coumaric, caffeic, chlorogenic, ferulic, 3,4-dimethoxycinnamic and 2,4,5-trimethoxycinnamic acid) with five antibiotics (amikacin, ampicillin, ciprofloxacin, erythromycin and vancomycin) against gram-negative (escherichia coli, enterobacter aerogenes and pseudomonas aerugi ... | 2010 | 20724513 |
| in vitro ciprofloxacin resistance profiles among gram-negative bacteria isolated from clinical specimens in a teaching hospital. | to evaluate the trends of ciprofloxacin resistance pattern in commonly isolated gram-negative bacteria over time in a saudi arabian teaching hospital. | 2010 | 20726190 |
| microbial transformation of albendazole. | screening scale studies were performed to biotransform anthelmintic drug albendazole by using twelve bacterial strains representing six genera and five actinomycetes cultures. among the cultures studied, bacillus subtilis mtcc 619, escherichia coli mtcc 118 and klebsiella pneumoniae mtcc 109 could transform albendazole to one metabolite whereas, enterobacter aerogenes ncim 2695, klebsiella aerogenes ncim 2258, pseudomonas aeruginosa ncim 2074 and streptomyces griseus ncim 2622 could transform al ... | 2010 | 20726341 |
| genetics and regulation of the major enzymes of alanine synthesis in escherichia coli. | genetic analysis of alanine synthesis in the model genetic organism escherichia coli has implicated avta, the still uncharacterized alaa and alab genes, and probably other genes. we identified alaa as yfbq. we then transferred mutations in several transaminase genes into a yfbq mutant and isolated a mutant that required alanine for optimal growth. for cells grown with carbon sources other than pyruvate, the major alanine-synthesizing transaminases are avta, yfbq (alaa), and yfdz (which we design ... | 2010 | 20729367 |
| high-dose ciprofloxacin for serious gram-negative infection in an obese, critically ill patient receiving continuous venovenous hemodiafiltration. | to describe the pharmacokinetic profile and clinical outcome associated with high-dose ciprofloxacin therapy in a patient with the triad of extreme obesity, multiple organ failure, and deep-seated infection. | 2010 | 20736424 |
| antioxidant, antitumor and antimicrobial activities of the crude extract and compounds of the root bark of allanblackia floribunda. | context: allanblackia floribunda oliver (guttiferae) is an african medicinal plant used traditionally to treat a variety of ailments. objective: we investigated the antitumor, radical scavenging, antimycobacterial, antibacterial and antifungal activities of the root bark extract of a. floribunda and three isolated phenolics, namely 1,7-dihydroxyxanthone (1), morelloflavone (2) and 7'-o-glucoside of morelloflavone (3). materials and methods: the 2,2-diphenyl-1-picryl-hydrazyl-hydrate (dpph) radi ... | 2011 | 20738222 |
| prevalence of microorganisms of hygienic interest in an organized abattoir in mumbai, india. | the magnitude of food-borne illnesses in india is unknown because of lack of surveillance networks. monitoring the prevalence of food-borne pathogens and indicators of contamination in primary production at abattoirs is imperative for creating a data bank and for effective control of such pathogens before they enter the food chain. | 2010 | 20818094 |
| phytoremediation of mercury in pristine and crude oil contaminated soils: contributions of rhizobacteria and their host plants to mercury removal. | the rhizospheric soils of three tested legume crops: broad beans (vicia faba), beans (phaseolus vulgaris) and pea (pisum sativum), and two nonlegume crops: cucumber (cucumis sativus) and tomato, (lycopersicon esculentum) contained considerable numbers (the magnitude of 10(5)g(-1) soil) of bacteria with the combined potential for hydrocarbon-utilization and mercury-resistance. sequencing of the 16s rrna coding genes of rhizobacteria associated with broad beans revealed that they were affiliated t ... | 2010 | 20833430 |
| hexavalent chromium reduction by bacterial consortia and pure strains from an alkaline industrial effluent. | to characterize the bacterial consortia and isolates selected for their role in hexavalent chromium removal by adsorption and reduction. | 2010 | 20854455 |
| novel chalcones and 1,3,5-triphenyl-2-pyrazoline derivatives as antibacterial agents. | novel sixteen chalcones and thirteen 1,3,5-triphenyl-2-pyrazolines were synthesized and characterized using ft-ir, hr-mass, nmr (¹h-nmr, ¹³c-nmr, 135 dept, ¹h-¹h cosy and ¹h and ¹³c cosy) and xrd. these compounds were evaluated for their antibacterial activity against six micro-organisms, namely bacillus subtilis ncim 2718, staphylococcus aureus ncim 5021, salmonella typhi ncim 2501, enterobacter aerogenes ncim 5139, pseudomonas aeruginosa ncim 5029, and proteus vulgaris ncim 2813 by twofold dil ... | 2010 | 20925692 |
| aerobic growth at nanomolar oxygen concentrations. | molecular oxygen (o(2)) is the second most abundant gas in the earth's atmosphere, but in many natural environments, its concentration is reduced to low or even undetectable levels. although low-oxygen-adapted organisms define the ecology of low-oxygen environments, their capabilities are not fully known. these capabilities also provide a framework for reconstructing a critical period in the history of life, because low, but not negligible, atmospheric oxygen levels could have persisted before t ... | 2010 | 20974919 |
| an outbreak of klebsiella pneumoniae and enterobacter aerogenes bacteremia after interventional pain management procedures, new york city, 2008. | in october 2008, an investigation was conducted into a cluster of gram-negative bloodstream infections after invasive pain management procedures at an outpatient facility to identify additional cases and determine the source of illness. | 2010 | 20975462 |
| a note on ph tolerance of aerobacter aerogenes and aerobacillus macerans as related to natural ecology and decomposition of acid food products. | | 1946 | 21018699 |
| production and properties of 2,3-butanediol; the effects of surface-volume ratio and reduced pressure on the fermentation of carbohydrates by aerobacillus polymyxa and aerobacter aerogenes. | | 1946 | 21022926 |
| isolation of inducible amp c beta-lactamase producing enterobacter aerogenes from a diabetic foot ulcer. | | 2010 | 21033310 |
| trends in production of extended-spectrum beta-lactamases among enterobacteriaceae of clinical interest: results of a nationwide survey in belgian hospitals. | to assess the frequency and diversity of extended-spectrum β-lactamases (esbls) in enterobacteriaceae isolates in belgium. | 2010 | 21036771 |
| raman spectroscopy of xylitol uptake and metabolism in gram-positive and gram-negative bacteria. | visible-wavelength raman spectroscopy was used to investigate the uptake and metabolism of the five-carbon sugar alcohol xylitol by gram-positive viridans group streptococcus and the two extensively used strains of gram-negative escherichia coli, e. coli c and e. coli k-12. e. coli c, but not e. coli k-12, contains a complete xylitol operon, and the viridans group streptococcus contains an incomplete xylitol operon used to metabolize the xylitol. raman spectra from xylitol-exposed viridans group ... | 2010 | 21037297 |
| raman spectroscopy of xylitol uptake and metabolism in gram-positive and gram-negative bacteria. | visible-wavelength raman spectroscopy was used to investigate the uptake and metabolism of the five-carbon sugar alcohol xylitol by gram-positive viridans group streptococcus and the two extensively used strains of gram-negative escherichia coli, e. coli c and e. coli k-12. e. coli c, but not e. coli k-12, contains a complete xylitol operon, and the viridans group streptococcus contains an incomplete xylitol operon used to metabolize the xylitol. raman spectra from xylitol-exposed viridans group ... | 2010 | 21037297 |
| antibacterial activity of the latex of argemone ochroleuca sweet. | to investigate the antibacterial effect of the crude latex of argemone ochroleuca (a. ochroleuca) as antibacterial potential against a range of human pathogenic bacteria. | 2010 | 21063649 |
| virulence properties and integron-associated antibiotic resistance of klebsiella mobilis strains isolated from clinical specimens. | this study examined klebsiella mobilis isolates cultured from clinical specimens for virulence-associated properties and antibiotic resistance. the strains produced a number of siderophores, including enterobactin, aerobactin and yersiniabactin. all isolates were able to adhere to and invade epithelial cells. they had cytotoxic activity, which caused destruction of human laryngeal epithelial hep-2 cells and evoked lysis of murine macrophage j774 cells. analyses of hep-2 and j774 cellular morphol ... | 2010 | 21088128 |