| murine pulmonary surfactant sp-a gene: cloning, sequence, and transcriptional activity. | sp-a is an abundant pulmonary surfactant-associated protein whose expression is controlled in a cell- and developmental-specific manner. to analyze regulation of sp-a gene expression, the murine sp-a gene was cloned and sequenced. the murine dba/2j gene was approximately 4.6 kb in length comprised of six exons and five introns. three mrnas of 3.0, 1.7, and 0.9 kb were detected by northern blot analysis of murine lung mrna. expression of the sp-a mrnas was first detected at day 15 of gestation an ... | 1992 | 1443158 |
| surfactant protein c is recycled from the alveoli to the lamellar bodies. | to clarify the life cycle of surfactant protein c (sp-c), we obtained alveolar surfactant from 3-day-old rabbits killed 15 h after the tracheal administration of 32p and l-[35s]methionine. small amounts of this surfactant were administered into the tracheas of 3-day-old rabbits, which were killed after 1 min and 2 and 4 h. we then analyzed the radioactivity associated with sp-c and with saturated phosphatidylcholine (dppc) in lung lavage fluid and in a lamellar body-rich fraction isolated from t ... | 1992 | 1443164 |
| synthesis of [2-3h-ethyl]s-(2-chloro-1,1,2-trifluoroethyl)-l-cysteine and its use in covalent-binding studies. | metabolism of s-(2-chloro-1,1,2-trifluoroethyl)-l-cysteine (ctfc) yields chlorofluorothioacetyl fluoride, which reacts with cellular proteins to form stable lysine adducts. little is known about the subcellular localization of these protein adducts or about their role in ctfc-induced nephrotoxicity. a method for the synthesis of ctfc and other cysteine s-conjugates labeled with 3h at the s-alkyl or s-alkenyl position would be useful in studies of s-conjugate metabolism and toxicity. reaction of ... | 1992 | 1443527 |
| expression and transfer of engineered catabolic pathways harbored by pseudomonas spp. introduced into activated sludge microcosms. | two genetically engineered microorganisms (gems), pseudomonas sp. strain b13 fr1(pfrc20p) (fr120) and pseudomonas putida kt2440(pwwo-eb62) (eb62), were introduced into activated sludge microcosms that had the level of aeration, nutrient makeup, and microbial community structure of activated sludge reactors. fr120 contains an experimentally assembled ortho cleavage route for simultaneous degradation of 3-chlorobenzoate (3cb) and 4-methyl benzoate (4mb); eb62 contains a derivative tol plasmid-enco ... | 1992 | 1444370 |
| biodegradation of diphenyl ether and its monohalogenated derivatives by sphingomonas sp. strain ss3. | the bacterium sphingomonas sp. strain ss3, which utilizes diphenyl ether and its 4-fluoro, 4-chloro, and (to a considerably lesser extent) 4-bromo derivatives as sole sources of carbon and energy, was enriched from soil samples of an industrial waste deposit. the bacterium showed cometabolic activities toward all other isomeric monohalogenated diphenyl ethers. during diphenyl ether degradation in batch culture experiments, phenol and catechol were produced as intermediates which were then channe ... | 1992 | 1444384 |
| cloning and nucleotide sequence of the gene coding for aspartokinase ii from a thermophilic methylotrophic bacillus sp. | the structural gene coding for the lysine-sensitive aspartokinase ii of the methylotrophic thermotolerant bacillus sp. strain mga3 was cloned from a genomic library by complementation of an escherichia coli auxotrophic mutant lacking all three aspartokinase isozymes. the nucleotide sequence of the entire 2.2-kb psti fragment was determined, and a single open reading frame coding for the aspartokinase ii enzyme was found. aspartokinase ii was shown to be an alpha 2 beta 2 tetramer (m(r) 122,000) ... | 1992 | 1444390 |
| characterization of a novel pseudomonas sp. that mineralizes high concentrations of pentachlorophenol. | a pentachlorophenol (pcp)-mineralizing bacterium was isolated from polluted soil and identified as pseudomonas sp. strain ra2. in batch cultures, pseudomonas sp. strain ra2 used pcp as its sole source of carbon and energy and was capable of completely degrading this compound as indicated by radiotracer studies, stoichiometric release of chloride, and biomass formation. pseudomonas sp. strain ra2 was able to mineralize a higher concentration of pcp (160 mg liter-1) than any previously reported pc ... | 1992 | 1444401 |
| isolation and characterization of a fluorene-degrading bacterium: identification of ring oxidation and ring fission products. | an arthrobacter sp. strain, f101, able to use fluorene as the sole source of carbon and energy, was isolated from sludge from an oil refinery wastewater treatment plant. during growth in the presence of fluorene, four major metabolites were detected and isolated by thin-layer chromatography and high-performance liquid chromatography. 9-fluorenol, 9h-fluoren-9-one, and 3,4-dihydrocoumarin were identified by uv spectra, mass spectrometry, and 300-mhz proton nuclear magnetic resonance. the fourth m ... | 1992 | 1444405 |
| role of heterotrophic bacteria in complete mineralization of trichloroethylene by methylocystis sp. strain m. | biodegradation experiments with radioactively labeled trichloroethylene showed that 32% of the radioactive carbon was converted to glyoxylic acid, dichloroacetic acid and trichloroacetic acid and that the same percentage was converted to co2 and co after 140 h of incubation by a pure culture of a type ii methane-utilizing bacterium, methylocystis sp. strain m, isolated from a mixed culture, mu-81, in our laboratory. in contrast, these water-soluble (14c)trichloroethylene degradation products wer ... | 1992 | 1444420 |
| metabolism of hexadecyltrimethylammonium chloride in pseudomonas strain b1. | a bacterium (strain b1) utilizing hexadecyltrimethylammonium chloride as a carbon and energy source was isolated from activated sludge and tentatively identified as a pseudomonas sp. this bacterium only grew on alkyltrimethylammonium salts (c12 to c22) and possible intermediates of hexadecyltrimethylammonium chloride breakdown such as hexadecanoate and acetate. pseudomonas strain b1 did not grow on amines. simultaneous adaptation studies suggested that the bacterium oxidized only the alkyl chain ... | 1992 | 1444422 |
| binding of pulmonary surfactant protein a to galactosylceramide and asialo-gm2. | the binding of pulmonary surfactant protein a (sp-a) to glycolipids was examined in the present study. the direct binding of sp-a on a thin-layer chromatogram was visualized using 125i-sp-a as a probe. 125i-sp-a bound to galactosylceramide and asialo-gm2, but failed to exhibit significant binding to gm1, gm2, asialo-gm1, sulfatide, and forssman antigen. the study of 125i-sp-a binding to glycolipids coated onto microtiter wells also revealed that sp-a bound to galactosylceramide and asialo-gm2. s ... | 1992 | 1444464 |
| identification of arginyl residues involved in the binding of ferredoxin-nadp+ reductase from anabaena sp. pcc 7119 to its substrates. | ferredoxin-nadp+ reductase from the cyanobacterium anabaena sp. pcc 7119 was chemically modified by the alpha-dicarbonyl reagent phenylglyoxal. the studies of the inactivation by this compound, which is specific for arginyl residues, of both the diaphorase and nadph-cytochrome c reductase activities, characteristic of the enzyme, are indicative of the involvement of at least one group of this kind in the binding site of nadp+ and a second one implicated in the interaction with ferredoxin. after ... | 1992 | 1444467 |
| enhanced production of platelet-activating factor in stimulated rat leukocytes pretreated with triacsin c, a novel acyl-coa synthetase inhibitor. | triacsin c, a product of streptmyces sp. sk-1894, was previously reported as an inhibitor of long chain acyl-coa synthetase. pretreatment with triacsin c (500 nm) for 1h enhanced production of platelet-activating factor in rat neutrophils, followed by stimulation with a23187 or fmlp. amount of lyso-paf was also augumented. triacsin c alone did not increase paf content and did not modulate enzymatic activities of acytransferase, cholinephosphotransferase, acetylhydrolase, acetyltransferase or pho ... | 1992 | 1445360 |
| heat-resistant fungi in the soil. | the occurrence of heat-resistant fungi has been demonstrated in samples of soil from the slovak republic. the heat-resistant species isolated were byssochlamys nivea, dichotomomyces cejpii, eupenicillium baarnense, neosartorya fischeri, talaromyces avellaneus, tal. bacillisporus, tal. emersonii, tal. flavus, tal. trachyspermus, tal. wortmanii, botryotrichum piluliferum, gilmaniella humicola and nodulisporium sp. some of them were isolated for the first time from czechoslovakian soil. for the var ... | 1992 | 1445767 |
| photosystem ii function and integrity in spite of drastic protein changes in a conserved region of the d2 protein. | d1 and d2 are structurally related proteins forming the core of the photosystem ii reaction center. the two proteins have several loop regions including an extended stroma-exposed loop between transmembrane helix d and parallel helix de. this loop (the d-de loop) is phylogenetically conserved in both proteins. the role of the d-de loop in photosystem ii was studied in synechocystis sp. pcc 6803 by constructing a chimeric d2 protein in which the stroma-exposed loop of d1 replaced that of d2. in o ... | 1992 | 1445845 |
| site-directed mutagenesis of the cpa-1 protein of photosystem ii: alteration of the basic residue pair 384,385r to 384,385g leads to a defect associated with the oxygen-evolving complex. | the psbb gene encodes the intrinsic chlorophyll-a binding protein cpa-1 (cp-47), a component of photosystem ii in higher plants, algae, and cyanobacteria. oligonucleotide-directed mutagenesis was used to introduce mutations into a segment of the psbb gene encoding the large extrinsic loop region of cpa-1 in the cyanobacterium synechocystis sp. pcc 6803. altered psbb genes were introduced into a mutant recipient strain (del-1) of synechocystis in which the genomic psbb gene had been partially del ... | 1992 | 1445882 |
| structures of three new homotyrosine-containing microcystins and a new homophenylalanine variant from anabaena sp. strain 66. | a hepatotoxic strain of cyanobacterium anabaena sp. 66 was isolated from a hepatotoxic water bloom sample in lake kiikkara, finland. four cyclic heptapeptide hepatotoxins were isolated and purified by hplc from cultured cells of this organism. the structures of three new homotyrosine (hty) containing toxins, [dha7]microcystin-htyr (dha = dehydroalanine) (1), [d-asp3,dha7]microcystin-htyr (2), and [l-ser7]microcystin-htyr (3), were assigned, based upon amino acid analyses using both a waters pico ... | 1992 | 1446006 |
| suppression of nociceptive responses in parafascicular neurons by stimulation of substantia nigra: an analysis of related inhibitory pathways. | a total of 166 neurons in parafascicular nucleus (pf) were studied, 85 from intact animals, 72 following dorsal spinal cord transection (d.sp.c.x.), and 9 following complete transection of the spinal cord. two patterns of nociceptive responses were identified following noxious stimulation and these responses were classified as 'nociceptive-on' and 'nociceptive-off' neurons, respectively. the effects of stimulating the substantia nigra (sns) on the spontaneous and on the nociceptive evoked discha ... | 1992 | 1446222 |
| stillbirths, mummies, abortions, and early embryonic death. | stillbirths, mummies, abortions, and early embryonic death have a substantial impact on the profitability of a farm in both endemic and epidemic conditions. fetal death is highly dependent on stage of gestation. implantation occurs around day 14 postmating in sows, and fetal death of an entire litter at this time usually results in a regular return to service. if more than four embryos remain alive, the sow may go on to farrow normally. if fetal death occurs after implantation but before calcifi ... | 1992 | 1446274 |
| a laser flash absorption spectroscopy study of anabaena sp. pcc 7119 flavodoxin photoreduction by photosystem i particles from spinach. | electron transfer from p700 in photosystem i (psi) particles from spinach to anabaena sp. pcc 7119 flavodoxin has been studied using laser flash absorption spectroscopy. a non-linear protein concentration dependence of the rate constants was obtained, suggesting a two-step mechanism involving complex formation (k = 3.6 x 10(7) m-1.s-1) followed by intracomplex electron transfer (k = 270 s-1). the observed rate constants had a biphasic dependence on the concentrations of nacl or mgcl2, with maxim ... | 1992 | 1446742 |
| fasting hypochlorhydria with gram positive gastric flora is highly prevalent in healthy old people. | fifteen healthy old people mean age 84 years (range 80-91 years), were examined to assess the effect of advanced age on the microecology of the upper gastrointestinal tract. twelve of 15 (80%) were hypochlorhydric with ph 6.6 (0.3) (mean (sem) and a mean bacterial count of 10(8) colony forming units (cfu) per ml (range 10(5)-10(10)) in fasting gastric aspirate. normochlorhydric subjects had low counts (< or = 10(1) cfu/ml). the microbial flora was dominated by viridans streptococci, coagulase ne ... | 1992 | 1446855 |
| analysis of polar lipids from some representative enterobacteria, plesiomonas and acinetobacter by fast atom bombardment-mass spectrometry. | fast atom bombardment-mass spectrometry (fab-ms) was used to analyse lipid extracts of bacteria to assess its usefulness for analysing anionic phospholipids of potential chemotaxonomic value. the following micro-organisms were tested: acinetobacter calcoaceticus, acinetobacter sp., citrobacter freundii, enterobacter cloacae (2 strains), escherichia coli (3 strains), hafnia alvei, klebsiella oxytoca, klebsiella pneumoniae, morganella morganii, plesiomonas shigelloides, proteus mirabilis (3 strain ... | 1992 | 1447059 |
| resolution and some properties of enzymes involved in enantioselective transformation of 1,3-dichloro-2-propanol to (r)-3-chloro-1,2-propanediol by corynebacterium sp. strain n-1074. | during the course of the transformation of 1,3-dichloro-2-propanol (dcp) into (r)-3-chloro-1,2-propanediol [(r)-mcp] with the cell extract of corynebacterium sp. strain n-1074, epichlorohydrin (ech) was transiently formed. the cell extract was fractionated into two dcp-dechlorinating activities (fractions ia and ib) and two ech-hydrolyzing activities (fractions iia and iib) by tskgel deae-5pw column chromatography. fractions ia and ib catalyzed the interconversion of dcp to ech, and fractions ii ... | 1992 | 1447132 |
| cell surface carbohydrates of microaerobic, nitrogenase-active, continuous cultures of bradyrhizobium sp. strain 32h1. | a continuous culture system was developed to examine the cell surface carbohydrates of bradyrhizobium sp. strain 32h1. when cultures were shifted from aerobic to microaerobic growth conditions, nitrogenase activity was induced and extracellular polysaccharide levels were greatly reduced; however, the levels of cell-associated cyclic beta-1,6 -1,3 glucans were found to be essentially unchanged. | 1992 | 1447152 |
| synthesis and antimicrobial properties of 2h-pyran-3(6h)-one derivatives and related compounds. | the synthesis of several derivatives of 2h-pyran-3(6h)-ones and their michael adducts is described. phenylthio, benzenesulfonyl, p-acetylaminobenzenesulfonyl, and p-bromophenyl substituents are beneficial for activity against gram-positive bacteria. 2-[4-(phenylthio)phenyl]-2-methyl-6-methoxy-2h-pyran-3(6h)-one (8a) showed a minimum inhibitory concentration of 1.56 micrograms/ml against staphylococcus aureus atcc 2593, and 2-[4-(phenylthio)phenyl]-2-methyl-6-[(p-nitrobenzoyl)oxy]-2h-pyran-3 (6h) ... | 1992 | 1447718 |
| postoperative wound infections following myocutaneous flap surgery in spinal injury patients. | severe pressure ulcers in patients with spinal cord injury are frequently treated by using musculocutaneous (m-c) flap surgery. there have been few studies of the use of perioperative antibiotics to prevent postoperative infection in this setting. we reviewed 74 m-c flap surgeries in 53 patients (41 male and 12 female) from october 1989 for one year. the sites involved were ischial (31), sacral (24), trochanteric (18), deltoid (2), olecranon (1) and posterior thigh (1). an antibiotic was usually ... | 1992 | 1448302 |
| degradation of non-phenolic beta-o-4 lignin substructure model compounds by acinetobacter sp. | acinetobacter sp. utilized non-phenolic beta-o-4-model compounds, 2-methoxy-4-formylphenoxyacetic acid and veratrylglycerol-beta-guaiacyl ether (vge) as sole carbon source. vanillin, vanillic acid, protocatechuic acid and catechol were detected in the 2-methoxy-4-formylphenoxyacetic acid amended culture. veratryl alcohol, veratraldehyde, veratric acid, vanillic acid, protocatechuic acid, catechol and guaiacol were identified from veratrylglycerol-beta-guaiacyl ether culture. acinetobacter sp. pr ... | 1992 | 1448618 |
| two strains of sivmac show differential transactivation mediated by sequences in the promoter. | two infectious molecular clones of simian immunodeficiency virus, sivmac251 and sivmac239, have very different in vivo properties, sivmac239 being much more pathogenic than sivmac251. to assess whether the in vivo differences between the two viruses would be reflected in transcriptional rates in vitro, transcriptional activity in the presence of the transactivation protein tat was analyzed by transient transfection assays in hut-78 and u937 cells. whereas the two promoters had similar basal acti ... | 1992 | 1448914 |
| resistance to synthetic pyrethroid pour-on insecticides in strains of the sheep body louse bovicola (damalinia) ovis. | groups of sheep infested with strains of bovicola (damalinia) ovis were obtained from flocks either with a history of failure to control lice with synthetic pyrethroid (sp) pour-on insecticides, or from farms where sp compounds were not used. the sheep were treated according to the manufacturer's recommendations with registered "off-shears" sp formulations. all treatments were applied under ideal conditions with doses calculated on an individual body weight basis and applied to the dorsal mid-li ... | 1992 | 1449456 |
| protective effect of trifluoperazine on the mitochondrial damage induced by ca2+ plus prooxidants. | isolated rat liver mitochondria undergo extensive swelling and disruption of membrane potential when they accumulate ca2+ in the presence of a prooxidant such as diamide or t-butylhydroperoxide. the phenothiazinic drug trifluoperazine, at concentrations (15-35 microm) which do not inhibit respiration or the influx of ca2+ into mitochondria, significantly protected mitochondria against the deleterious effects of ca2+ plus a prooxidant. in contrast, at concentrations higher than 100 microm the dru ... | 1992 | 1449534 |
| [nosocomial infection in the neonatal period at a third level care center]. | results are presented on epidemiologic surveillance of nosocomial infection of all neonates attended at the instituto nacional de perinatologia throughout 1988-1990, reporting rates of 2.1, 2.9 and 5.5 per 100 discharge for each of the years studied. data are presented in full by service, showing rates of 4.7, 9.5 and 23.0 per 100 discharge in neonatal intensive care unit (nicu) and 1.5, 3.2 and 6.7 per 100 discharge for neonatal intermediate care (nic) for the same three years. with respect to ... | 1992 | 1449625 |
| persistence of increased cholesteryl ester in human skin fibroblasts is caused by residual exogenous sphingomyelinase and is reversed by phospholipid liposomes. | human skin fibroblasts (hsf) were exposed to sphingomyelinase 50 or 5 mu/ml for 60 min, washed with 5 mm edta or 20% serum and with phosphate-buffered saline, and postincubated for 24 h in the presence of [14c]16:0 sphingomyelin (sp) liposomes. the recovery of up to 48% of label in the medium in ceramide provided evidence of persistence of sphingomyelinase activity. the rate of hydrolysis of [14c]16:0 sp remained the same irrespective of whether the liposomes were added immediately after the was ... | 1992 | 1450209 |
| molecular characterization of a pea beta-1,3-glucanase induced by fusarium solani and chitosan challenge. | beta-glucanases are prominent proteins in pea endocarp tissue responding to fungal infection. we have cloned and sequenced a partial pea cdna clone, ppig312, corresponding to a beta-1,3-glucanase in pea pods challenged with the incompatible pathogen fusarium solani f. sp. phaseoli. the insert from the partial pea cdna was used to probe a genomic library derived from pea leaves of the same cultivar. one of the genomic clones, ppig4-3, contained the complete coding sequence for a mature beta-1,3-g ... | 1992 | 1450378 |
| a multivariate model to predict caries increment in montreal children aged 5 years. | a study was carried out in montreal (canada) to predict caries development over the period of one year in primary teeth of kindergarten children (mean age 5 years 8 months +/- 4 months) living in a non-fluoridated area. the 302 children were examined at school on two occasions, one year apart. at the first examination selected predictors were collected: caries experience, salivary s. mutans and lactobacilli, buffer capacity, debris index, parents' education, fluoride consumption and family struc ... | 1992 | 1451000 |
| a method for identifying causative chemicals of allergic contact dermatitis using a combination of chemical analysis and patch testing in patients and animal groups: application to a case of rubber boot dermatitis. | a 63-year-old woman developed allergic contact dermatitis from rubber boots. initial investigation, by patch testing in the patient and chemical analysis of the causative rubber boots, revealed that mercaptobenzothiazole (mbt) and dibenzothiazyl disulfide (mbts) were the causative chemicals. subsequent investigations were performed by patch testing in animal groups. an extract of the causative rubber boots, mbt and mbts were used for sensitization of guinea pigs by the guinea pig maximization te ... | 1992 | 1451462 |
| sequence of a rhodococcus gene encoding a protein with extensive homology to the mammalian propionyl-coa carboxylase beta chain. | sequence analysis of a 2-kilobase dna fragment from rhodococcus sp. ni86/21 revealed an open reading frame encoding a 476-amino-acid protein with striking homology to the rat and human propionyl-coa carboxylase beta subunits. the nucleotide sequence of a corresponding prokaryotic gene has not yet been reported. upstream, the c-terminal part of a putative beta-ketoacyl synthase was identified. | 1992 | 1452030 |
| the possibilities of changing the production of exocellular polysaccharides of a mutant mycobacterium strain. | by second-step mutagenesis and treatment with n-methyl-n'-nitro-n-nitrosoguanidine a mutant strain of mycobacterium sp. v-649 producing a glucan extracellular polymer and another new streptomycin-resistant mutant were prepared. this mutant strain formed more than 100% first-rate (1.0-1.2%) exocellular polysaccharide. treatment with 1% dimethyl sulfoxide during submerged cultivation of the mutant strain did not increase the production of the extracellular polysaccharide. | 1992 | 1452093 |
| [isolation and identification of a strain of pseudomonas sp. producing insoluble glucan hydrolase]. | a strain, cib871, isolated from the natural world can hydrolyze insoluble glucan(ig) produced by s. mutans. this organism has been identified to be a strain of pseudomonas sp. it grows well at 30 degrees c. the optimum medium for producing insoluble glucan hydrolase (igase) is composed of 0.3% peptone, 0.03% ig and m1 salt solution (nh4)2 so4 1mg/ml, mgso4 50 micrograms/ml, fecl2 50 micrograms/ml, k2hpo4 0.5mg/ml). the igase production reached maximum when cultured for 65-70 hours. | 1992 | 1452147 |
| evaluation of a new monoclonal antibody combination reagent for direct fluorescence detection of giardia cysts and cryptosporidium oocysts in human fecal specimens. | giardia lamblia and cryptosporidium parvum can cause severe symptoms in humans, particularly in the immunologically compromised. monoclonal antibody reagents offer increased sensitivity and an excellent alternative to conventional staining methods. these reagents are helpful when screening large numbers of patients or those with minimal symptoms. problems of false-positive and false-negative results with routine staining methods for stool parasites can be eliminated with monoclonal antibody reag ... | 1992 | 1452711 |
| a survey and review of hepatobiliary lesions in australian macropods. | this report presents information on the range of diseases and lesions that occurred in sections of livers of macropods held in the non-domestic animal registry at taronga zoo. of 142 affected livers, 52 were due to parasites, 24 to bacteria, nine to macropod herpesvirus, four to fungal agents and ten to tumours. in addition, 17 livers had acute degenerative or necrotic lesions, 22 were affected by fibrosis or other chronic lesions and four had miscellaneous degenerative lesions. common parasitic ... | 1992 | 1452809 |
| determination of t-pa activity and t-pa antigen in human milk. | although a considerable number of publications on tissue plasminogen activator (t-pa) in human milk have appeared, most investigators have determined t-pa activity using an assay on fibrin plates [1, 2, 3, 4]. we measured t-pa activity by a bioimmunoassay and t-pa antigen by elisa using a monoclonal antibody (sp-322) [6], and estimated t-pa index ((t-pa activity, ng/ml)/(t-pa antigen, ng/ml) x 100) in human colostrum and milk to evaluate the fluctuations of post-delivery t-pa. the concentrations ... | 1992 | 1453294 |
| maternity and medicinal plants in vanuatu. i. the cycle of reproduction. | ethnobotanical data collection to select pharmacologically active species was carried out within a clearly defined therapeutic context: those plants used during the course of a woman's reproductive life. extensive bibliographical and field data collection and cross-examination of the information thus gathered have provided us with a clearer picture of the effectiveness of these plant species. various concepts, behaviours and practices relating to menstruation, pregnancy, birth and birth control ... | 1992 | 1453707 |
| [plasmids for biodegradation of 2,6-dimethylpyridine, 2,4-dimethylpyridine, and pyridine in strains of arthrobacter]. | arthrobacter crysallopoietes strain km-4 degrading 2,6-dimethylpyridine and strain km-4a degrading both 2,6-dimethylpyridine and pyridine, arthrobacter sp. km-4b degrading 2,4-dimethylpyridine were isolated from soil. arthrobacter crystallopoietes km-4 and arthrobacter sp. km-4b contain 100 md plasmids pbs320 and pbs323. arthrobacter crystallopietes km-4a harbours a 100 md and 80 md plasmids. plasmid curing and conjugation transfer results confirm that these plasmids are involved in degradation ... | 1992 | 1454076 |
| the presence of neuropeptides in gabaergic and cholinergic rat cerebrocortical synaptosome sub-populations. | gabaergic and cholinergic synaptosomes from rat cerebral cortex were isolated by a magnetic immunoaffinity technique, i.e. immunomagnetophoresis. these subpopulations were extracted and subjected to radioimmunoassay for four neuropeptides: neuropeptide y (npy); vasoactive intestinal peptide (vip); substance p (sp); and somatostatin (srif). in each of the sub-populations three of the four peptides were enriched in the sorted fraction compared with the mother fraction with respect to the cytosolic ... | 1992 | 1454155 |
| [nematodirus nemorhaedi sp. n. (nematoda: trichostrongylidae) from the amur goral nemorhaedus caudatus raddeanus]. | nematodirus nemorhaedi sp. n. is described. the new species was found in the small intestine in three long-tailed gorals from the maritime territory. the species differs from other species of the genus, parasites of ungulates, by very large (over 1.5 mm) spicules and characteristic structure of their distal end in males; by long (about 1 mm) ovijector in females and by a specific combination of characters of synloph, genital bursa in males, sizes of body, structure of vulva, sizes and shape of e ... | 1992 | 1454413 |
| [the clinico-immunological subtypes of systemic lupus erythematosus]. | based on clinico-immunologic studies subtypes of systemic lupus erythematosus (sle) were distinguished. the anf-r++h-dna-ch50 variant determines acute onset of sle with renal injury in the form of diffuse glomerulonephritis. the anf-sp-rnp-rf mirrors the development of raynaud's syndrome, sjögren's syndrome, polymyositis, pneumosclerosis and myocarditis. the anf-sp-ro-rf variant is associated with skin derangement in the form of discoid foci, anular, papulosquamous eruption, vitiligo, hyperpigme ... | 1992 | 1455378 |
| human helminthosis in a rural community of plateau state, nigeria. | urine and faecal samples were obtained from 1,517 people in fier, a typical rural village in plateau state, nigeria, for a parasitological survey among the population. 643 (42.39%) persons were found to be infected with altogether 9 helminths, namely: ascaris lumbricoides, hookworm, taenia sp., trichuris trichiura, enterobius vermicularis, schistosoma mansoni, s. haematobium, hymenolepis nana and strongyloides stercoralis. age and religion as opposed to sex, type of sewage system, and type of ho ... | 1992 | 1456465 |
| effects of 2,4-dichlorophenoxyacetic acid on rhizobium sp. membrane fluidity. | the effects of 1 mm 2,4-dichlorophenoxyacetic acid on rhizobium sp. in pure culture was studied. in a previous work it was demonstrated that this herbicide produces an alteration on the saturated to unsaturated phospholipid acyl chains ratio. in the present paper, it was found that this alteration produces a modification on the membrane fluidity in bacteria that had achieved the stationary phase. the same results were obtained when determinations were done in liposomes from sonicated lipids of t ... | 1992 | 1456775 |
| [antibacterial effects of gamma-interferon in experimental klebsiella infection]. | the results of the experimental study on the effect of the natural and recombinant gamma-interferons (gamma-ifs) of mice on the process of the infection caused by klebsiella sp. are presented. the infection was reproduced by intraperitoneal contamination of mice with a virulent culture of klebsiella pneumoniae 5055, line shk. the gamma-ifs were administered to the animals in a dose of 250 units per mouse on days 1 and 3 after the contamination. survival of the animals, clearance of the pathogen ... | 1992 | 1456806 |
| sulfated polyester interactions with the cd4 molecule and with the third variable loop domain (v3) of gp120 are chemically distinct. | sulfated polyesters (sp) that inhibit hiv infection interact with both the gp120 binding region of cd4 molecules and with the v3 domain loop of gp120 molecules (gp120/v3) but the contributions of these interactions to the inhibition of hiv env-mediated fusion are presently unclear. in order to characterize the molecular mechanisms by which sp inhibit hiv env-mediated fusion, we studied the effect of sp treatment on env-mediated fusion of cd4+ cells driven by recombinant vaccinia virus (vpe-16) a ... | 1992 | 1457206 |
| antibacterial activity of essential oil components. | antibacterial activity of fifteen essential oil components towards food borne staphylococcus sp., micrococcus sp., bacillus sp. and enterobacter sp. was studied by an agar plate technique. cinnamic aldehyde was the most active compound followed by citral, geraniol, eugenol and menthol. at 500 micrograms/ml, cinnamic aldehyde completely inhibited the bacterial growth for more than 30 days at 30 degrees c that was comparable to 200 micrograms/ml of butylated hydroxy anisole (bha). at lower tempera ... | 1992 | 1457292 |
| the major glycolipid recognized by sp-d in surfactant is phosphatidylinositol. | surfactant protein d (sp-d), a multimeric calcium-dependent lectin isolated from pulmonary alveolar lavage, has been previously shown to interact reversibly with crude surfactant [persson et al. (1990) j. biol. chem. 265, 5755-5760]. in this study, sp-d is shown to interact reversibly with a preparation of organelles enriched in lamellar bodies, in a manner inhibited by calcium-chelating agents and by competing saccharides. an interaction with an endogenous glycoprotein could not be identified b ... | 1992 | 1457414 |
| restriction mapping and localization of gl-7-aca acylase gene. | this paper presents the results about the restriction mapping of recombinant plasmids pmr5 and pmr6 containing gl-7-aca acylase gene from pseudomonas sp. 130, gene localization and its expression under the control of different promoters, tet, tac or lac/tac, in escherichia coli. the analysis of gel electrophoresis of pmr5 cleaved with several kinds of restriction enzymes indicated that there is no sites of ecori, hindiii and clai but the presence of following sites: one hpai, two xhoi, three eam ... | 1992 | 1457717 |
| alterations to the permeability of plant and animal mitochondria submitted to ca2+ releasing agents. | 1. mitochondria from different rat tissues and from plants were compared as regards their sensitivity towards ca2+ in the presence of different ca2+ releasing agents, and the phospholipase a2 activity was evaluated in the different mitochondrial preparations. 2. the mitochondria were exposed to ca2+ and an oxidant such as t-butylhydroperoxide or diamide or to ca2+ and inorganic phosphate, and plant mitochondria were seen to be much more resistant than liver, brain or kidney mitochondria of rats ... | 1992 | 1458833 |
| tetronothiodin, a novel cckb receptor ligand, antagonizes cholecystokinin-induced ca2+ mobilization in a pituitary cell line. | we found a novel nonpeptide cckb receptor antagonist, tetronothiodin (ro 09-1468), in the culture broth of streptomyces sp. nr0489. the structure of the compound (c31o8h38s), which has a 19-membered ring with an alpha-acyltetronic acid and tetrahydrothiophene moiety, is completely different from that of any known cck receptor antagonist. tetronothiodin inhibited [125i]cck-8 binding to rat brain cckb receptors with an ic50 of 3.6 nm, whereas it showed only weak affinity for rat ccka receptors (ic ... | 1992 | 1459194 |
| mineralization of 2-chloro- and 2,5-dichlorobiphenyl by pseudomonas sp. strain ucr2. | pseudomonas sp. strain ucr2 was isolated from a multi-chemostat mating experiment between a chlorobenzoate-degrader, pseudomonas aeruginosa strain jb2, and a chlorobiphenyl-degrader, arthrobacter sp. strain b1barc. strain ucr2 differed from either of the parental organisms in that it grew on both 2-chloro- and 2,5-dichlorobiphenyl with concomitant release of chloride. phenotypic typing by the biolog system indicated that strain ucr2 shared greater similarity with strain jb2 (88%) than strain b1b ... | 1992 | 1459405 |
| synergistic growth studies of entamoeba gingivalis using an ecologen. | a unique multiple diffusion growth chamber, an ecologen, designed for the study of interactions among microorganisms, was introduced as a means of growing xenic cultures of entamoeba gingivalis with crithidia sp. or yersinia enterocolitica. entamoeba gingivalis was grown in the central diffusion reservoir of the ecologen connected to separate growth chambers inoculated with the microorganisms to be evaluated. growth of the accompanying bacteria in the e. gingivalis compartment was almost complet ... | 1992 | 1459786 |
| structure and expression of a cyanobacterial ilvc gene encoding acetohydroxyacid isomeroreductase. | acetohydroxyacid isomeroreductase (ahair) is the shared second enzyme in the biosynthetic pathways leading to isoleucine and valine. ahair is encoded by the ilvc gene in bacteria. a 1,544-bp fragment of genomic dna containing the ilvc gene was cloned from the cyanobacterium synechocystis sp. strain pcc 6803, and the complete nucleotide sequence was determined. the identity of the gene was established by comparison of the nucleotide and derived peptide sequences with those of other ilvc genes. th ... | 1992 | 1459938 |
| a new nylon oligomer degradation gene (nylc) on plasmid poad2 from a flavobacterium sp. | flavobacterium sp. strain ki725 harbors plasmid poad21, a derivative of nylon oligomer-degradative plasmid poad2, in which all of nyla (the gene for 6-aminohexanoate cyclic dimer hydrolase [ei]) was deleted but nylb (the gene for 6-aminohexanoate dimer hydrolase [eii]) was retained. ki725 showed no growth on unfractionated nylon oligomers (nom1) obtained from a nylon factory as a sole carbon and nitrogen source (nom1 minimum plate). extracts of ki725 cells possessed hydrolytic activity for nom1 ... | 1992 | 1459943 |
| purification and characterization of the hydantoin racemase of pseudomonas sp. strain ns671 expressed in escherichia coli. | the hydantoin racemase gene of pseudomonas sp. strain ns671 had been cloned and expressed in escherichia coli. hydantoin racemase was purified from the cell extract of the e. coli strain by phenyl-sepharose, deae-sephacel, and sephadex g-200 chromatographies. the purified enzyme had an apparent molecular mass of 32 kda as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. by gel filtration, a molecular mass of about 190 kda was found, suggesting that the native enzyme is a ... | 1992 | 1459947 |
| purification and characterization of a tetrachloro-p-hydroquinone reductive dehalogenase from a flavobacterium sp. | tetrachloro-p-hydroquinone (tech) is the first intermediate in pentachlorophenol (pcp) degradation by flavobacterium sp. strain atcc 39723. we previously purified a pcp hydroxylase that oxidized pcp to tech. subsequently, we identified the reductive dehalogenation of tech to 2,3,6-trichloro-p-hydroquinone and then to 2,6-dichloro-p-hydroquinone in a cell extract with the reduced form of glutathione as the reducing agent under anaerobic conditions. here we report the purification of a tech reduct ... | 1992 | 1459949 |
| the influence of flanking sequence on the o-glycosylation of threonine in vitro. | to investigate the influence of flanking amino acid sequence on the o-glycosylation of a single threonine residue in vitro, we have examined a series of 52 related peptides. the substrates were based upon a sequence from human von willebrand factor which is known to be glycosylated in vivo (-6phmaqvtvgpgl+5). each residue of the parent peptide was substituted, in turn, with isoleucine, alanine, proline, glutamic acid, or arginine. peptides were glycosylated using a udp-galnac:polypeptide n-acety ... | 1992 | 1460004 |
| core mutations of synechococcus sp. pcc 7002 phycobilisomes: a spectroscopic study. | three cyanobacterial strains harboring mutations affecting phycobilisome (pbs) cores were studied using steady state absorption and fluorescence and time-resolved fluorescence. the apcf mutant, missing beta 18, and the apcdf mutant, missing both alpha apb and beta 18, showed only small spectroscopic differences from the wild-type strain; their pbs emission was blue shifted by 10 nm, whereas their absorption spectra and time-resolved fluorescence kinetics were virtually unchanged. the third mutan ... | 1992 | 1460543 |
| [systemic chronic candidiasis following typhlitis caused by candida albicans]. | typhlitis is an infrequent infectious complication which may appear during a period of intense granulocytopenia, generally in patients with acute leukemia. the most common causal germs are gram negative bacilli although the importance of candida sp. as an etiologic agent of this disease is ever more frequent. the case of a 14 years old patient with acute lymphoblastic leukemia who, after chemotherapy treatment, presented typhlitis by candida albicans followed by chronic systemic candidiasis (csc ... | 1992 | 1460914 |
| impairment of growth of leishmania donovani by trypanosoma brucei during co-culture. | cells of leishmania donovani in co-culture with trypanosoma brucei, were severely affected in their growth, resulting in swelling and subsequent lysis. similar effects were also observed when crithidia luciliae or phytomonas sp. were co-cultured with t. brucei. direct contact between the cells under investigation and t. brucei was necessary because t. brucei did not hamper the growth of the other trypanosomatids, when separated by a filter with 0.2 microns pore size. examination of this phenomen ... | 1992 | 1461680 |
| [peptidergic innervation in the sinus hair follicles of several mammalian species]. | in this study, the detailed distribution of peptidergic nerve fibers in the sinus hair follicle was immunohistochemically investigated by the avidin biotin-complex method using antibodies against calcitonin gene-related peptide (cgrp), substance p (sp), vasoactive intestinal polypeptide (vip) and neuropeptide y (npy) in the cat, dog, hamster, rat, mouse and guinea pig. the peptide-immunoreactive (ir) nerve fibers presented a fine varicose appearance. they entered the sinus hair follicle after pe ... | 1992 | 1462755 |
| a survey of the intestinal helminths of refugees in juba, sudan. | a parasitological survey of refugees based in juba, sudan, involving 241 faecal samples, revealed that 66% of the population harboured intestinal helminths. the most commonly found infection was hookworm (36%), followed by schistosoma mansoni (26%), strongyloides stercoralis (20%), hymenolepis nana (11%), ascaris lumbricoides (1.2%), trichuris trichiura (0.8%) and taenia sp. (0.4%). many of the specimens (42%) harboured a single infection, 21% had double, 2% triple and 1% quadruple infections. p ... | 1992 | 1463359 |
| identification of a second psac gene in the cyanobacterium synechocystis sp. pcc6803. | the psac gene encodes the 9 kda protein subunit of photosystem i that carries the iron-sulfur centers fa and fb. the paper describes the sequence and transcription analysis of a second psac gene (psac2) from synechocystis sp. pcc6803. thepsac2 gene is transcribed into an abundant monocistronic mrna. the deduced amino acid sequence of psi-c2 is very similar to the protein sequences from two other cyanobacteria (synechococcus sp. pcc7002 and nostoc sp. pcc8009). in contrast, the recently isolated ... | 1992 | 1463835 |
| cloning and transcription analysis of the ndh(a-i-g-e) gene cluster and the ndhd gene of the cyanobacterium synechocystis sp. pcc6803. | the plastid dna of higher plants contains eleven reading frames that are homologous to subunits of the mitochondrial nadh-ubiquinone oxidoreductase (complex i). the genes are expressed, but a plastid nad(p)h dehydrogenase has not yet been isolated and the function of the enzyme in plastid metabolism is unknown. cyanobacteria also contain a nadh dehydrogenase that is homologous to the mitochondrial complex i. the enzyme is sensitive to rotenone and is located on the cytoplasmic and the thylakoid ... | 1992 | 1463844 |
| purification, properties, and partial amino acid sequence of chitinase from a marine alteromonas sp. strain o-7. | chitinase (ec 3.2.1.14) was isolated from the culture supernatant of a marine bacterium, alteromonas sp. strain o-7. the enzyme (chi-a) was purified by anion-exchange chromatography (deae-toyopearl 650 m) and gel filtration (sephadex g-100). the purified enzyme showed a single band on sodium dodecyl sulfate polyacrylamide gel electrophoresis. the molecular size and pi of chi-a were 70 kda and 3.9, respectively. the optimum ph and temperature of chi-a were 8.0 and 50 degrees c, respectively. chi- ... | 1992 | 1464065 |
| glyphosate degradation by immobilized bacteria: laboratory studies showing feasibility for glyphosate removal from waste water. | to evaluate immobilized bacteria technology for the removal of low levels of glyphosate (n-phosphonomethylglycine) from aqueous industrial effluents, microorganisms with glyphosate-degrading activity obtained from a fill and draw enrichment reactor inoculated with activated sludge were first exposed to glyphosate production wastes containing 500-2000 mg glyphosate/l. the microorganisms were then immobilized by adsorption onto a diatomaceous earth biocarrier contained in upflow plexiglas columns. ... | 1992 | 1464067 |
| evaluation of two test procedures for diagnosis of giardiasis in dogs. | two commercially available tests, an antigen-capture elisa for use on fecal samples, and a peroral nylon string test for use in dogs, were compared with a zinc sulfate fecal concentration technique (zsct) for detection of giardiasis in dogs. of 77 dogs and 164 fecal samples (from these dogs), 33 and 52, respectively were found to be giardia-positive on the basis of results of the zsct. the elisa gave false-negative results for 10 and 14% of zsct-positive dogs and fecal samples, respectively, and ... | 1992 | 1466497 |
| [focal urinary salmonella infection]. | a case of focal salmonella sp. urinary infection in an elderly diabetic patient is described herein. the therapeutic approach in this uncommon type of infection is discussed. | 1992 | 1466587 |
| photoperiodic response of pineal-thyroid axis of the female indian palm squirrel, funambulus pennanti. | effect of short (sp: 10 l: 14 d: light: dark) and long (lp: 14 l: 10 d) photoperiod was tested on pineal-thyroid axis in a tropical seasonally breeding rodent f. pennanti during sexually regressive phase of reproduction. sp reduced thyroid weight and plasma thyroxine (t4) as well as increased melatonin (amt) while pinealectomy (px) prevented such an effect of sp. lp and px plus lp had no significant effect on thyroid activity when compared with their respective controls. these results suggest th ... | 1992 | 1466877 |
| [anomalies produced by cytochalasin b after 10.5 days of gestation: in vitro study]. | 124 rats embryos (rattus norvegicus sp.) have been cultured for 48 hours period (from 9.5 days to 11.5 days). 24 hours after the beginning of the culture, cytochalasin b (cb) dissolved in 1% dimethylsulphoxide (dmso) was added to the culture medium in the experimental group (61 embryos), whereas only dmso was added to the medium in the control group (63 embryos). survival rate in the control group was 100% whereas in cb-treated embryos the higher the dose, the lesser the survival rate. embryo gr ... | 1992 | 1467565 |
| noncariogenicity of erythritol as a substrate. | erythritol is a sugar alcohol produced by aureobasidium sp. from glucose. it is 75-80% as sweet as sucrose and is also nonhygroscopic. the aim of this study was to evaluate this sugar substitute from a cariological point of view. erythritol was neither utilized as a substrate for the lactic acid production nor for plaque formation of mutans streptococci (serotypes a-h) and certain oral microorganisms. it was not utilized for water-insoluble glucan synthesis or cellular adherence by glucosyltrans ... | 1992 | 1468100 |
| prevalence of corynebacteria in diabetic foot infections. | microbiological flora of diabetic foot infections are usually polymicrobial and frequently include bacteria of the corynebacterium sp. (diphtheroids). the purpose of this study was to determine the prevalence of these bacteria in both deep and superficial cultures in diabetic patients with foot infections. | 1992 | 1468282 |
| developmental regulation of surfactant-associated proteins in rabbit fetal lung in vivo. | the developmental regulation of the rabbit surfactant-associated proteins, sp-a, sp-b, and sp-c, was investigated using northern blot analysis. these proteins comprise approximately 10% by weight of pulmonary surfactant, a lipoprotein secreted by type ii cells that reduces surface tension at the air-alveolar interface. sp-a mrna and sp-b mrna were first detected in rabbit fetal lung at day 24 of gestation (term = 31 days), i.e., approximately two days prior to the appearance of lamellar bodies w ... | 1992 | 1468410 |
| cochinmicins, novel and potent cyclodepsipeptide endothelin antagonists from a microbispora sp. i. production, isolation, and characterization. | cochinmicins i, ii, iii are novel peptolides produced in submerged-fermentation cultures of microbispora sp. atcc 55140. these closely related compounds are separated by hplc and are novel competitive endothelin antagonists. cochinmicins ii and iii are stereoisomeric to each other. cochinmicin i is the deschloro analog of cochinmicin iii. | 1992 | 1468977 |
| cochinmicins, novel and potent cyclodepsipeptide endothelin antagonists from a microbispora sp. ii. structure determination. | cochinmicins i, ii, and iii are competitive endothelin antagonists produced by microbispora sp. atcc 55140. the cochinmicins are cyclic depsipeptides containing six alpha-amino acids and a pyrrolecarboxylic acid. based upon ms, 1d and 2d nmr, and lc data, the structures and absolute stereochemistries of the cochinmicins have been assigned. all three components have the same basic sequence and contain one equivalent each of d-allo-threonine, d-alanine, l-phenylalanine, d-phenylalanine, 5-chloropy ... | 1992 | 1468978 |
| biosynthesis of benastatin a. | the biosynthesis of benastatin a, produced by streptomyces sp. mi384-df12, has been studied by feeding experiments with 14c- and 13c-labeled compounds followed by measurement of radioactivity and 13c nmr analysis. the results indicate that benastatin a is derived from two methionine units and fourteen acetate units, condensed in the "head-to-tail" fashion of typical polyketide biosynthesis. | 1992 | 1468985 |
| additional cochinmicins from a microbispora sp. | | 1992 | 1468988 |
| amygdala neurons in vitro: neurite growth and effects of estradiol. | dissociated cell cultures from embryonic rat medial amygdala were studied using sequential photography and immunocytochemical staining for cytoskeletal proteins and substance p (sp). cultures were seeded with cells taken from fetuses grouped by sex; experimental cultures were raised in medium containing 17-beta-estradiol (e2). forty-eight hours after plating a few neurons begin to define their morphological polarity by the differentiation of an axon-like process; at 5 days in vitro (div) almost ... | 1992 | 1469745 |
| isolation of promoter sequences from brevibacterium sp. r312. | promoter sequences recognized by escherichia coli rna polymerase were isolated from brevibacterium sp. r312, a coryneform strain producing nitrile hydratase and amidase. ten escherichia coli clones containing promoter sequences were selected for their ability to grow with chloramphenicol concentrations of up to 1500 micrograms/ml. the strength of these promoter sequences was determined. we carried out a preliminary study of the strongest promoter having a chloramphenicol acetyl-transferase/beta- ... | 1992 | 1471439 |
| purification, characterization and function of dihydrolipoamide dehydrogenase from the cyanobacterium anabaena sp. strain p.c.c. 7119. | a dihydrolipoamide dehydrogenase (dihydrolipoamide: nad+ oxidoreductase, ec 1.8.1.4) (dld) has been found in the soluble fraction of cells of both unicellular (synechococcus sp. strain p.c.c. 6301) and filamentous (calothrix sp. strain p.c.c. 7601 and anabaena sp. strain p.c.c. 7119) cyanobacteria. dld from anabaena sp. was purified 3000-fold to electrophoretic homogeneity. the purified enzyme exhibited a specific activity of 190 units/mg and was characterized as a dimeric fad-containing protein ... | 1992 | 1471997 |
| the consequences of doxorubicin quinone reduction in vivo in tumour tissue. | a clear role for quinone reduction in the mechanism of action of doxorubicin has still to be established. there are three possible outcomes of this form of doxorubicin metabolism: (1) drug free radical formation, redox cycling and generation of reactive oxygen species (ros) resulting in lipid peroxidation and dna damage; (2) covalent binding of reactive drug intermediates to dna; and (3) formation of an inactive 7-deoxyaglycone metabolite. in this work, the occurrence of each of these pathways h ... | 1992 | 1472081 |
| the enzymology of doxorubicin quinone reduction in tumour tissue. | we have reported previously that enzymes present in the sp 107 rat mammary carcinoma catalyse doxorubicin quinone reduction (qr) to 7-deoxyaglycone metabolites in vivo [willmott and cummings, biochem pharmacol 36: 521-526, 1987]. in order to provide insights into the role of qr in the antitumour mechanism of action of doxorubicin, we have attempted in this work to identify the enzyme(s) responsible. nad(p)h: (quinone acceptor) oxidoreductase (dt-diaphorase) was the major quinone reductase in the ... | 1992 | 1472082 |
| the primary structure of providencia rettgeri penicillin g amidase gene and its relationship to other gram negative amidases. | the nucleotide sequence of penicillin g amidase (pa,e.c.3.5.1.11) of providencia rettgeri was determined. we aligned our p. rettgeri pa with other known gram negative periplasmically located beta-lactam amidases. the analysis revealed a high homology with other enterobacteric amidases (60%-65%), while with similar pseudomonas sp. amidases the homology exceeded 25%. these homologies indicate their common ancestry. | 1992 | 1472713 |
| [diagnosis of caprine contagious pleuropneumonia: recent improvements]. | due to the difficulty in isolating mycoplasma sp. type f38, two techniques for rapid detection are proposed in order to identify the presence of mycoplasmas of the mycoides group in samples of pleural fluid. a modification in the composition of isolation media promotes the growth of type f38 mycoplasmas and inhibits the growth of m. ovipneumoniae strains. | 1992 | 1472732 |
| [can the reactions of lysine oxidative deamination be used for the biochemical differentiation between salmonella sp. and citrobacter freundii?]. | | 1992 | 1472785 |
| the 5s ribosomal rna gene is linked to large and small subunit ribosomal rna genes in the oomycetes, phytophthora vignae, p. cinnamomi, p. megasperma f.sp. glycinea and saprolegnia ferax. | southern hybridization and polymerase chain reaction data indicate that the 5s ribosomal rna gene is linked to the ribosomal rna gene repeat unit in the oomycetes, phytophthora vignae, p. cinnamomi, p. megasperma f.sp. glycinea and saprolegnia ferax, and is apparently transcribed in the same direction as the large and small subunit ribosomal rna genes. the polymerase chain reaction has been used to amplify all components of the entire ribosomal rna gene repeat unit for each of these oomycetes. t ... | 1992 | 1473177 |
| syntheses and activities of n-substituted derivatives of siastatin b. | n-substituted derivatives of siastatin b have been obtained by a chemical modification. some derivatives showed potent inhibitory activities against streptococcus sp. and clostridium perfringens neuraminidases. | 1992 | 1473994 |
| glisoprenins, new inhibitors of acyl-coa: cholesterol acyltransferase produced by gliocladium sp. fo-1513. ii. structure elucidation of glisoprenins a and b. | the structure of glisoprenins a and b, novel acyl-coa: cholesterol acyltransferase (acat) inhibitors, was determined by spectroscopic analyses, mainly 1h and 13c nmr and ms. glisoprenin a was deduced to be a tetrahydroxynonaprenol and glisoprenin b to be an oxidative modification of glisoprenin a. | 1992 | 1473995 |
| exposure to psoroptes sp. mites is common among bighorn sheep (ovis canadensis) populations in california. | sera (n = 806) from 50 populations of bighorn sheep (ovis canadensis) in california (usa) were evaluated for antibodies to psoroptes sp. mites using a kinetic enzyme-linked immunosorbent assay (elisa). test values for each sample were determined to be either positive or negative at each of two elisa cutoff values that provided either 100% sensitivity (low cutoff) or 100% specificity (high cutoff), respectively. one hundred sixty-eight (20.8%) sera were seropositive at the low cutoff value, and 8 ... | 1992 | 1474651 |
| [directed autoselection of bacteria during turbidostat cultivation]. | a method has been proposed for directed bacteria autoselection, based on their sensitivity to growth inhibitors during continuous unlimited cultivation. a smooth increase of a growth inhibitor concentration in the medium has been used in response to the appearance and autoselection of more resistant strains. the aim can be reached by continuous measurements of the specific microbial growth rate during the process and its keeping at the required level by the inhibitor additions. the experimental ... | 1992 | 1474942 |
| a degu-containing sp beta prophage complements superactivator mutations affecting the bacillus subtilis degsu operon. | the bacillus subtilis degsu two-gene operon encodes a two-component regulatory system which positively controls expression of a variety of genes encoding degradative enzymes. point superactivator mutations in either degs or degu increase the production of these enzymes. a specialized transducing sp beta phage which partially complements several of these mutations was isolated from a phage library of the b. subtilis chromosome. this phage was shown to contain the degu (wild-type) gene. this unexp ... | 1992 | 1475517 |
| further monogeneans from iranian freshwater fishes. | thirteen dactylogyrus, 1 dogelius and 1 tetraonchus species are first recorded on iranian fishes. of them, twelve dactylogyrus species and tetraonchus monenteron are identified with known species. dogelius persicus sp. n. from barbus sharpeyi, barbus grypus and carassobarbus luteus, as well as dactylogyrus holciki from chalcalburnus mossulensis and c. chalcoides are described as new species. besides the first record of the above monogeneans, new host records are given to some known species. | 1992 | 1476091 |
| [isolation of 2 coccidia of the genus isospora from ducks in france]. | the authors isolated 2 isospora species from ducks: isospora mandari and isospora sp. this was the first report of the genus isospora from the duck in france. one of the 2 isolated species (isospora sp) has not been described in the duck before. | 1992 | 1476409 |
| exploitation of gene(s) involved in 2,4-diacetylphloroglucinol biosynthesis to confer a new biocontrol capability to a pseudomonas strain. | tn5 mutagenesis and complementation analysis were used to clone a 6-kb genomic fragment required for biosynthesis of 2,4-diacetylphloroglucinol (phl) from fluorescent pseudomonas sp. strain f113. a recombinant plasmid, pcu203, containing this region partially complemented a phl production-negative mutant (f113g22) derived from strain f113. when sugar beet seeds were sown into an unsterilized soil, in which sugar beet was subject to damping-off by pythium ultimum, the emergence of sugar beet seed ... | 1992 | 1476431 |
| cloning and sequence analysis of genes for dehalogenation of 4-chlorobenzoate from arthrobacter sp. strain su. | strains of arthrobacter catalyze a hydrolytic dehalogenation of 4-chlorobenzoate (4-cba) to p-hydroxybenzoate. the reaction requires atp and coenzyme a (coa), indicating activation of the substrate via a thioester, like that reported for pseudomonas sp. strain cbs3 (j. d. scholten, k.-h. chang, p. c. babbit, h. charest, m. sylvestre, and d. dunaway-mariano, science 253:182-185, 1991). the dehalogenase genes of arthrobacter sp. strain su were cloned and expressed in escherichia coli. analyses of ... | 1992 | 1476446 |