| molecular and biochemical analyses of cbcel9a/cel48a, a highly secreted multi-modular cellulase by caldicellulosiruptor bescii during growth on crystalline cellulose. | during growth on crystalline cellulose, the thermophilic bacterium caldicellulosiruptor bescii secretes several cellulose-degrading enzymes. among these enzymes is cela (cbcel9a/cel48a), which is reported as the most highly secreted cellulolytic enzyme in this bacterium. cbcel9a/cel48a is a large multi-modular polypeptide, composed of an n-terminal catalytic glycoside hydrolase family 9 (gh9) module and a c-terminal gh48 catalytic module that are separated by a family 3c carbohydrate-binding mod ... | 2013 | 24358340 |
| differential activity of autochthonous bacteria in controlling drought stress in native lavandula and salvia plants species under drought conditions in natural arid soil. | the effectiveness of autochthonous plant growth-promoting rhizobacteria was studied in lavandula dentata and salvia officinalis growing in a natural arid mediterranean soil under drought conditions. these bacteria identified as bacillus megaterium (bm), enterobacter sp. (e), bacillus thuringiensis (bt), and bacillus sp. (bsp). each bacteria has different potential to meliorate water limitation and alleviating drought stress in these two plant species. b. thuringiensis promoted growth and drought ... | 2014 | 24337805 |
| complete genome of bacillus megaterium siphophage slash. | the complete annotated genome sequence of bacillus megaterium bacteriophage slash is described here. several key features related to morphogenesis, replication/recombination, dna metabolism, and lysis are described. slash also encodes a homolog of sleb, a germination-specific cell wall amidase. | 2013 | 24336363 |
| improvement of thermal stability of a mutagenised α-amylase by manipulation of the calcium-binding site. | site-directed mutagenesis of an α-amylase isolated from bacillus megaterium who has been performed to evaluate the roles of the calcium binding site residues in enzyme thermostability. the strategy used was to replace residues in the hypothetical calcium binding loops of b. megaterium who α-amylase (bmw-amylase) by equivalent positions at halothermothrix orenii α-amylase (amya) as a thermophilic amylase by quikchange site directed mutagenesis. asn-75, ser-76, and his-77 were mutated in the secon ... | 2013 | 24315644 |
| significant improvement of thermal stability of glucose 1-dehydrogenase by introducing disulfide bonds at the tetramer interface. | rational design was applied to glucose 1-dehydrogenase (lsgdh) from lysinibacillus sphaericus g10 to improve its thermal stability by introduction of disulfide bridges between subunits. one out of the eleven mutants, designated as ds255, displayed significantly enhanced thermal stability with considerable soluble expression and high specific activity. it was extremely stable at ph ranging from 4.5 to 10.5, as it retained nearly 100% activity after incubating at different buffers for 1h. mutant d ... | 2013 | 24315638 |
| antimicrobial activities of some euphorbia species. | in this study, the antimicrobial activities of methanolic extracts and latex of some euphorbia species used for medical purposes in turkey were investigated. the extracts of euphorbia aleppica l., euphorbia szovitsii fisch.&mey. var. harputensis aznav. ex m. s. khan, euphorbia falcata l. sub. falcata var. falcata, euphorbia denticulata lam., euphorbia macroclada boiss., euphorbia cheiradenia boiss.&hohen, euphorbia virgata waldst.&kit., euphorbia petiolata banks&sol. were prepared with methanol. ... | 2013 | 24311840 |
| complete genome of bacillus megaterium siphophage staley. | siphophage staley was isolated because of its ability to grow on bacillus megaterium. here we report the complete genome and annotation of phage staley and describe core features. among its interesting genes is one encoding an sleb germination protein. | 2013 | 24309730 |
| complete genome of bacillus megaterium podophage pony. | bacillus megaterium podophage pony was isolated from a soil sample collected in college station, tx. here, we report the sequencing and annotation of the 39,844-bp genome of phage pony and describe the major features identified. | 2013 | 24309727 |
| isotope effects and temperature dependences in the action of the glucose dehydrogenase of the mesophilic bacterium bacillus megaterium. | the glucose dehydrogenase of the mesophilic bacterium bacillus megaterium (optimal growth around 35 °c) exhibits non-linear eyring temperature dependences from 25 to 55 °c in its catalysis of the oxidation by hydride-transfer to nad(+) of the β-anomers of 1-h-d-glucose and 1-d-d-glucose (rate constant kcat/kmβ). a break around 300k separates a high-t region from a low-t region. in the high-t region, isotopic enthalpies of activation within a considerable experimental error are equal to zero. in ... | 2013 | 24298200 |
| cyclization of squalene from both termini: identification of an onoceroid synthase and enzymatic synthesis of ambrein. | the onoceroids are triterpenoids biosynthesized from squalene or (3s)-2,3-oxidosqualene by cyclization from both termini. we recently revealed that tetraprenyl-β-curcumene cyclase from bacillus megaterium (bmetc) is a bifunctional triterpene/sesquarterpene cyclase that converts head-to-tail tetraprenyl-β-curcumene and tail-to-tail squalene into pentacyclic and bicyclic products, respectively, in vivo. here, we reveal that bmetc has an unprecedented catalytic function in cyclizing squalene from b ... | 2013 | 24274794 |
| bioprospection of bacteria and yeasts from atlantic rainforest soil capable of growing in crude-glycerol residues. | the increasing world production of biodiesel has resulted in an accumulation of crude glycerol as the major byproduct. this could be used as carbon source for industrial microbiology, with economic and environmental advantages for the biodiesel industry. we explored an atlantic rainforest soil sample to search for crude glycerol-degrading microorganisms. microcosms of this soil were established containing minimal medium + 8% crude glycerol (w/w); the biological activity was measured by respirome ... | 2013 | 24222222 |
| design and synthesis of cationic antibacterial peptide based on leucrocin i sequence, antibacterial peptide from crocodile (crocodylus siamensis) white blood cell extracts. | leucrocin i is an antibacterial peptide isolated from crocodile (crocodylus siamensis) white blood cell extracts. based on leucrocin i sequence, cationic peptide, ny15, was designed, synthesized and evaluated for antibacterial activity against bacillus sphaericus tistr 678, bacillus megaterium (clinical isolate), vibrio cholerae (clinical isolate), salmonella typhi (clinical isolate), salmonella typhi atcc 5784 and escherichia coli 0157:h7. the efficacy of the peptide made from all l-amino acids ... | 2014 | 24192554 |
| development of a minimal chemically defined medium for ketogulonicigenium vulgare wsh001 based on its genome-scale metabolic model. | commercial production of 2-keto-l-gulonic acid (2-klg), the immediate precursor of l-ascorbic acid, is by ketogulonicigenium vulgare in co-culture with bacillus megaterium. we used flux balance analysis (fba) to study a genome-scale metabolic model (gsmm) of k. vulgare, iwz663, and found that k. vulgare is deficient in nutrient biosynthetic pathways. individually omitting l-glycine, l-cysteine, l-methionine, l-tryptophan, adenine, thymine, thiamine and pantothenate from complete chemically defin ... | 2014 | 24172253 |
| in vitro interaction between bacillus megaterium strains and caco-2 cells. | to further our understanding of the virulence potential of bacillus megaterium strains, cell association and invasion assays were conducted in vitro by infecting human enterocytes (caco-2 cells) with 53 strains of this bacterium isolated from honey. two series of experiments were performed: (i) necrosis and cell detachment assays with the supernatants of bacterial culture filtrates from 16-h cultures and (ii) adhesion/invasion assays in which cultured enterocytes incubated with bacteria from 3-h ... | 2013 | 24151779 |
| isolation, purification and characterisation of an organic solvent-tolerant ca2+-dependent protease from bacillus megaterium au02. | a new organic solvent-tolerant strain bacillus megaterium au02 which secretes an organic solvent-tolerant protease was isolated from milk industry waste. statistical methods were employed to achieve optimum protease production of 43.6 u/ml in shake flask cultures. the productivity of the protease was increased to 53 u/ml when cultivated under controlled conditions in a 7-l fermentor. the protease was purified to homogeneity by a three-step process with 24 % yield and specific activity of 5,375 u ... | 2014 | 24122712 |
| the identification of the nitrate assimilation related genes in the novel bacillus megaterium nct-2 accounts for its ability to use nitrate as its only source of nitrogen. | bacillus megaterium nct-2 is a novel bacterium that can utilize nitrate as its only nitrogen source for growth.the nitrate assimilation related genes that are involved in this process would be expected to be crucial. however, little is known about the genomic background of this bacterium,let alone the sequences of the nitrate assimilation related genes. in order to further investigate the nitrate assimilation function of the nct-2, genome sequencing was performed.after obtaining the fine map of ... | 2014 | 24122235 |
| a single mutation at the ferredoxin binding site of p450 vdh enables efficient biocatalytic production of 25-hydroxyvitamin d(3). | vitamin d3 hydroxylase (vdh) from pseudonocardia autotrophica is a cytochrome p450 monooxygenase that catalyzes the two-step hydroxylation of vitamin d3 (vd3 ) to produce 25-hydroxyvitamin d3 (25(oh)vd3 ) and 1α,25-dihydroxyvitamin d3 (1α,25(oh)2 vd3 ). these hydroxylated forms of vd3 are useful as pharmaceuticals for the treatment of conditions associated with vd3 deficiency and vd3 metabolic disorder. herein, we describe the creation of a highly active t107a mutant of vdh by engineering the pu ... | 2013 | 24115473 |
| siderophore production by bacillus megaterium: effect of growth phase and cultural conditions. | siderophore production by bacillus megaterium was detected, in an iron-deficient culture medium, during the exponential growth phase, prior to the sporulation, in the presence of glucose; these results suggested that the onset of siderophore production did not require glucose depletion and was not related with the sporulation. the siderophore production by b. megaterium was affected by the carbon source used. the growth on glycerol promoted the very high siderophore production (1,182 μmol g(-1) ... | 2014 | 24101562 |
| comparison of (bio-)transformation methods for the generation of metabolite-like compound libraries of p38α map kinase inhibitors using high-resolution screening. | four hydrophobic p38α mitogen-activated protein kinase inhibitors were refluxed with 7.5% hydrogen peroxide at 80°c and irradiated with visible light in order to generate more hydrophilic conversion products. the resulting mixtures were analyzed in a high-resolution screening (hrs) platform, featuring liquid chromatographic separation coupled in parallel with a fluorescence enhancement based continuous-flow affinity bioassay towards the p38α mitogen-activated protein kinase and with high-resolut ... | 2014 | 24090642 |
| basic studies on the role of components of bacillus megaterium as flotation biocollectors in sulphide mineral separation. | studies were carried out to assess the utility of the cellular and extracellular constituents of bacillus megaterium for the flotation of sphalerite and galena minerals. based on the flotation results on the individual minerals, it was observed that sphalerite was preferentially floated compared to galena. a maximum selectivity index (si) value of 11.7 was achieved in the presence of the soluble fraction of the thermolysed cells, which was higher than that obtained with the intact cells (si of 6 ... | 2014 | 24085394 |
| the mechanism of copper uptake by tyrosinase from bacillus megaterium. | tyrosinase belongs to the type 3 copper enzyme family, containing a dinuclear copper center, cua and cub. it is mainly responsible for melanin production in a wide range of organisms. although copper ions are essential for the activity of tyrosinase, the mechanism of copper uptake is still unclear. we have recently determined the crystal structure of tyrosinase from bacillus megaterium (tyrbm) and revealed that this enzyme has tighter binding of cua in comparison with cub. investigating copper a ... | 2013 | 24061559 |
| electrochemistry of mammalian cytochrome p450 2b4 indicates tunable thermodynamic parameters in surfactant films. | electrochemical methods continue to present an attractive means for achieving in vitro biocatalysis with cytochromes p450; however understanding fully the nature of electrode-bound p450 remains elusive. herein we report thermodynamic parameters using electrochemical analysis of full-length mammalian microsomal cytochrome p450 2b4 (cyp 2b4) in didodecyldimethylammonium bromide (ddab) surfactant films. electronic absorption spectra of cyp 2b4-ddab films on silica slides reveal an absorption maximu ... | 2013 | 24013063 |
| the flavin-containing reductase domain of cytochrome p450 bm3 acts as a surrogate for mammalian nadph-p450 reductase. | cytochrome p450 bm3 (cyp102a1) from bacillus megaterium is a self-sufficient monooxygenase that consists of a heme domain and fad/fmn-containing reductase domain (bmr). in this report, the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (mtt) and 5-cyano-2,3-ditolyl tetrazolium chloride (ctc) by bmr was evaluated as a method for monitoring bmr activity. the electron transfer proceeds from nadph to bmr and then to bmr substrates, mtt and ctc. mtt and ctc are monotetrazol ... | 2012 | 24009851 |
| polyhydroxybutyrate production from oil palm empty fruit bunch using bacillus megaterium r11. | oil palm empty fruit bunch (opefb), contains abundant cellulose and hemicelluloses and can be used as a renewable resource for fuel and chemical production. this study, as the first attempt, aims to convert opefb derived sugars to polyhydroxybutyrate (phb). opefb collected from a malaysia palm oil refinery plant was chemically pretreated and enzymatically hydrolyzed by an in-house prepared cellulase cocktail. the phb producer, bacillus megaterium r11, was isolated in singapore and could accumula ... | 2013 | 24001560 |
| microbially induced selective flotation of sphalerite from galena using mineral-adapted strains of bacillus megaterium. | the selective flotation of sphalerite from a sphalerite-galena mineral mixture has been achieved using cells and extracellular secretions of bacillus megaterium after adaptation to the chosen minerals. the extracellular secretions obtained after thermolysis of bacterial cells adapted to sphalerite yield the highest flotation recovery of sphalerite with a selectivity index value of 24.5, in comparison to the other cellular and extra-cellular bio-reagents studied. the protein profile for the unada ... | 2013 | 24001447 |
| development of a tunable wide-range gene induction system useful for the study of streptococcal toxin-antitoxin systems. | despite the plethora of genetic tools that have been developed for use in streptococcus mutans, the s. mutans genetic system still lacks an effective gene induction system exhibiting low basal expression and strong inducibility. consequently, we created two hybrid gene induction cassettes referred to as xyl-s1 and xyl-s2. both xyl-s cassettes are xylose inducible and controlled by the bacillus megaterium xylose repressor. the xyl-s cassettes each demonstrated >600-fold-increased reporter activit ... | 2013 | 23934493 |
| elucidation of the anaerobic pathway for the corrin component of cobalamin (vitamin b12). | it has been known for the past 20 years that two pathways exist in nature for the de novo biosynthesis of the coenzyme form of vitamin b12, adenosylcobalamin, representing aerobic and anaerobic routes. in contrast to the aerobic pathway, the anaerobic route has remained enigmatic because many of its intermediates have proven technically challenging to isolate, because of their inherent instability. however, by studying the anaerobic cobalamin biosynthetic pathway in bacillus megaterium and using ... | 2013 | 23922391 |
| a versatile mini-mazf-cassette for marker-free targeted genetic modification in bacillus subtilis. | there are some drawbacks for mazf-cassette constructed in previous reports for marker-free genetic manipulation in bacillus subtilis, including cloning-dependent methodology and non-strictly controlled expression system. in our study, the modifications on mazf-cassette are carried out, such as using mini zeocin resistance gene as positive-selectable marker and strictly controlled xyl promoter from the b. subtilis to replace non-strictly controlled iptg-inducible pspac or xyl promoter from bacill ... | 2013 | 23911571 |
| crystallization and preliminary x-ray characterization of the tetrapyrrole-biosynthetic enzyme porphobilinogen deaminase from bacillus megaterium. | the enzyme porphobilinogen deaminase (pbgd; hydroxymethylbilane synthase; ec 2.5.1.61) catalyses an early step of the tetrapyrrole-biosynthesis pathway in which four molecules of the monopyrrole porphobilinogen are condensed to form a linear tetrapyrrole. the enzyme possesses a dipyrromethane cofactor which is covalently linked by a thioether bridge to an invariant cysteine residue. expression in escherichia coli of a his-tagged form of bacillus megaterium pbgd permitted the crystallization and ... | 2013 | 23908040 |
| improvement of ni phytostabilization by inoculation of ni resistant bacillus megaterium sr28c. | the use of metal tolerant plants for the phytostabilization of metal contaminated soil is an area of extensive research and development. in this study the effects of inoculation of ni-resistant bacterial strains on phytostabilization potential of various plants, including brassica juncea, luffa cylindrica and sorghum halepense, were studied. a ni-resistant bacterial strain sr28c was isolated from a nickel rich serpentine soil and identified as bacillus megaterium based on the morphological featu ... | 2013 | 23895909 |
| two-helper-strain co-culture system: a novel method for enhancement of 2-keto-l-gulonic acid production. | a novel two-helper-strain co-culture system (tscs) was developed to enhance 2-keto-l-gulonic acid (2-klg) productivity for vitamin c production. bacillus megaterium and b. cereus (with a seeding culture ratio of 1:3, v/v), used as helper strains, increased the 2-klg yield using ketogulonigenium vulgare compared to the conventional one-helper-strain (either b. cereus or b. megaterium) co-culture system (oscs). after 45 h cultivation, 2-klg concentration in the tscs (69 g l(-1)) increased by 8.9 a ... | 2013 | 23881329 |
| a new cytochrome p450 system from bacillus megaterium dsm319 for the hydroxylation of 11-keto-β-boswellic acid (kba). | in the genome of bacillus megaterium dsm319, a strain who has recently been sequenced to fully exploit its potential for biotechnological purposes, we identified a gene encoding the cytochrome p450 cyp106a1 as well as genes encoding potential redox partners of cyp106a1. we cloned, expressed, and purified cyp106a1 and five potential autologous redox partners, one flavodoxin and four ferredoxins. the flavodoxin and three ferredoxins were able to support the activity of cyp106a1 displaying the firs ... | 2014 | 23797329 |
| bacillus megaterium mediated mineralization of calcium carbonate as biogenic surface treatment of green building materials. | microbially induced calcium carbonate precipitation is a biomineralization process that has various applications in remediation and restoration of range of building materials. in the present study, calcifying bacteria, bacillus megaterium ss3 isolated from calcareous soil was applied as biosealant to enhance the durability of low energy, green building materials (soil-cement blocks). this bacterial isolate produced high amounts of urease, carbonic anhydrase, extra polymeric substances and biofil ... | 2013 | 23793943 |
| peptide-based communication system enables escherichia coli to bacillus megaterium interspecies signaling. | the use of mixtures of microorganisms, or microbial consortia, has the potential to improve the productivity and efficiency of increasingly complex bioprocesses. however, the use of microbial consortia has been limited by our ability to control and coordinate the behaviors of microorganisms in synthetic communities. synthetic biologists have previously engineered cell-cell communication systems that employ machinery from bacterial quorum-sensing (qs) networks to enable population-level control o ... | 2013 | 23775238 |
| optimization of polyhydroxybutyrate production by marine bacillus megaterium msbn04 under solid state culture. | a marine sponge-associated bacterium bacillus megaterium msbn04 was used for the production of polyhydroxybutyrate (phb) under solid state culture (ssc). a central composite design (ccd) was employed to optimize the production medium and to find out the interactive effects of four independent variables, viz. tapioca industry waste, palm jaggery, horse gram flour and trace element solution on phb production. the maximum yield of phb 8.637 mg g(-1) of substrate (tapioca industry waste) was achieve ... | 2013 | 23748002 |
| a novel melt stable and high tensile strength biopolymer (polyhydroxyalkanoates) from bacillus megaterium (mtcc10086) and its characterization. | in the present investigation, we have defined a novel biopolymer from bacillus megaterium strain with novel melt stability, high tensile strength, and elongation to break properties higher to polypropylene and similar to polyethylene the polymers available commercially. the polymer was characterized with ftir and xrd. the percent crystalinity was found to 44.09% with tensile strength 42 (mpa) and elongation to break (%) 142 higher than polypropylene. the polymeric properties were confirmed by di ... | 2014 | 23744753 |
| ynea mrna instability is involved in temporary inhibition of cell division during the sos response of bacillus megaterium. | the sos response, a mechanism enabling bacteria to cope with dna damage, is strictly regulated by the two major players, reca and lexa (bacillus homologue dinr). genetic stress provokes formation of ssdna-reca nucleoprotein filaments, the coprotease activity of which mediates the autocatalytic cleavage of the transcriptional repressor dinr and ensures the expression of a set of din (damage-inducible) genes, which encode proteins that enhance repair capacity, accelerate mutagenesis rate and cause ... | 2013 | 23728628 |
| tandem mass spectrometry study of p38α kinase inhibitors and related substances. | the p38 mitogen-activated protein kinase α (p38α) is an important drug target widely investigated for therapy of chronic inflammatory diseases. its inhibitors are rather lipophilic and as such not very favourable lead compounds in drug discovery. therefore, we explored various approaches to access new chemical space, create diversity, and generate lead libraries with improved solubility and reduced lipophilicity, based on known p38α inhibitors, e.g., birb796 and tak-715. compound modification st ... | 2013 | 23722963 |
| production of l-lactic acid by the yeast candida sonorensis expressing heterologous bacterial and fungal lactate dehydrogenases. | polylactic acid is a renewable raw material that is increasingly used in the manufacture of bioplastics, which offers a more sustainable alternative to materials derived from fossil resources. both lactic acid bacteria and genetically engineered yeast have been implemented in commercial scale in biotechnological production of lactic acid. in the present work, genes encoding l-lactate dehydrogenase (ldh) of lactobacillus helveticus, bacillus megaterium and rhizopus oryzae were expressed in a new ... | 2013 | 23706009 |
| indigenous plasmids of bacillus megaterium wsh-002 involved in mutualism with ketogulonicigenium vulgare wsh-001. | in the two-step vitamin c fermentation process, the precursor 2-keto-l-gulonic acid (2-klg) was synthesized using a mixed culture of ketogulonicigenium vulgare wsh-001 and bacillus megaterium wsh-002, which contained three plasmids, pbme1, pbme2 and pbme3. the cell growth of b. megaterium was not affected by the elimination of these plasmids. however, elimination of pbme2 and pbme3 significantly affected l-sorbose uptake and 2-klg production. sequence analysis of the plasmids showed that many of ... | 2013 | 23688502 |
| studies on production of fructo-oligosaccharides (fos) by gamma radiation processing of microbial levan. | microbial levan, a natural polymer of fructose, was produced and purified by alcohol precipitation from culture supernatants of bacillus megaterium type 1 grown in an optimized liquid sucrose medium. gpc analysis showed that the yield of the major fraction of levan having molecular weight ~5000 d increased with increase in sucrose concentration in the broth. levan subjected to (60)co-gamma radiation as well as acid hydrolysis was investigated by rheometry, uv-visible spectrophotometry and gel pe ... | 2013 | 23688493 |
| antibacterial, antidiarrhoeal, and cytotoxic activities of methanol extract and its fractions of caesalpinia bonducella (l.) roxb leaves. | caesalpinia bonducella is an important medicinal plant for its traditional uses against different types of diseases. therefore, the present study investigated the antimicrobial, antidiarrhoeal, and cytotoxic activities of the methanol extract and ethyl acetate, chloroform, and petroleum ether (pet. ether) fractions of c. bonducella leaves. | 2013 | 23663985 |
| biomineralization of calcium carbonate polymorphs by the bacterial strains isolated from calcareous sites. | microbially induced calcium carbonate precipitation (miccp) is a naturally occurring biological process that has various applications in remediation and restoration of a range of building materials. in the present investigation, five ureolytic bacterial isolates capable of inducing calcium carbonate precipitation were isolated from calcareous soils on the basis of production of urease, carbonic anhydrase, extrapolymeric substances, and biofilm. bacterial isolates were identified as bacillus mega ... | 2013 | 23648862 |
| cryptosporioptide: a bioactive polyketide produced by an endophytic fungus cryptosporiopsis sp. | an antibiotic polyketide, cryptosporioptide (1) was isolated from the culture extract of the endophytic fungus cryptosporiopsis sp. the structure of cryptosporioptide has been established with the help of 1d ((1)h, (13)c), 2d nmr (hsqc, hmbc, cosy, noesy) techniques and mass spectrometry (fabms, hrfabms). the absolute configuration was established by means of electronic circular dichroism (ecd). cryptosporioptide exhibited both lipoxygenase inhibitory and anti-bacillus megaterium activities. | 2013 | 23642454 |
| investigating the functional hierarchy of bacillus megaterium pv361 spore germinant receptors. | spores of bacillus megaterium qm b1551 germinate rapidly when exposed to a number of single-trigger germinant compounds, including glucose, proline, leucine, and certain inorganic salts. however, spores of strain pv361, a plasmidless qm b1551 derivative that lacks the geru germinant receptor (gr) responsible for mediating germination in response to single-trigger compounds, can germinate efficiently when incubated in nutritionally rich media, presumably via activation of additional germinant rec ... | 2013 | 23625848 |
| identification of new proteins that modulate the germination of spores of bacillus species. | a number of operons encoding the nutrient germinant receptors (grs) in dormant spores of bacillus megaterium and bacillus subtilis species have small open reading frames (orfs) of unknown function within or immediately adjacent to the operons. inactivation of the genes in these orfs, encoding proteins now termed d proteins, either significantly increased or decreased spore germination via the associated gr but had no effects on germination via non-gr-dependent germinants. these effects on gr-dep ... | 2013 | 23625846 |
| improvement of biocontrol of damping-off and root rot/wilt of faba bean by salicylic acid and hydrogen peroxide. | rhizoctonia solani, fusarium solani, f. oxysporum, and macrophomina phaseolina were found to be associated with root rott and wilt symptoms of faba bean plants collected from different fieldes in new valley governorate, egypt. all the obtained isolates were able to attack faba bean plants (cv. giza 40) causing damping-off and root rot/wilt diseases. r. solani isolates 2 and 5, f. solani isolate 8, f. oxysporum isolate 12 and m. phaseolina isolate 14 were the more virulent ones in the pathogenici ... | 2013 | 23610539 |
| degumming of ramie fiber and the production of reducing sugars from waste peels using nanoparticle supplemented pectate lyase. | banana, citrus and potato peels were subjected to treatment with hydroxyapatite nanoparticle (np) supplemented purified pectate lyase (np-pl), isolated from bacillus megaterium ak2 to produce reducing sugar (rs). at both 50 and 90°c production of rs by np-pl was almost twofold greater than that by untreated pectate lyase (pl) from each of the three peels. the optimal production of rs from banana and citrus peels were after 24 and 6h of incubation while it was 24 and 4h for potato peels at 50 and ... | 2013 | 23587821 |
| development of a biologically based fertilizer, incorporating bacillus megaterium a6, for improved phosphorus nutrition of oilseed rape. | sustainable methods with diminished impact on the environment need to be developed for the production of oilseed rape in china and other regions of the world. a biological fertilizer consisting of bacillus megaterium a6 cultured on oilseed rape meal improved oilseed rape seed yield (p < 0.0001) relative to the nontreated control in 2 greenhouse pot experiments using natural soil. this treatment resulted in slightly greater yield than oilseed rape meal without strain a6 in 1 of 2 experiments, sug ... | 2013 | 23586746 |
| bacillus subtilis edms (formerly pgse) participates in the maintenance of episomes. | extrachromosomal dna maintenance (edm) is an important process in molecular breeding and for various applications in the construction of genetically engineered microbes. here we describe a novel bacillus subtilis gene involved in edm function called edms (formerly pgse). functional gene regions were identified using molecular genetics techniques. we found that edms is a membrane-associated protein that is crucial for edm. we also determined that edms can change a plasmid vector with an unstable ... | 2013 | 23583563 |
| compensation effect of bacterium containing biofertilizer on the growth of cucumis sativus l. under al-stress conditions. | biofertilizers are used to improve soil fertility and plant production in sustainable agriculture. however, their applicability depends on several environmental parameters. the aim of our study was to evaluate the effect of free-living bacteria containing fertilizer on the growth of cucumber (cucumis sativus l. cvs. delicates) under aluminium (al) stress. different responses to al stress of cucumber growth parameters were examined in terms of root elongation and physiological traits, such as spa ... | 2013 | 23567831 |
| impact of carbon source and variable nitrogen conditions on bacterial biosynthesis of polyhydroxyalkanoates: evidence of an atypical metabolism in bacillus megaterium dsm 509. | twenty bacterial strains were examined on their ability to produce polyhydroxyalkanoates (pha) from different carbon sources under rich and depleted nitrogen conditions. preliminary experiments with glucose as sole carbon source allowed to select pha producing bacteria using ftir spectroscopy. they were further tested with eight additional carbon substrates including organic, fatty acids or sugars. pha content and monomer composition of four chosen strains (pseudomonas putida mt-2, bacillus mega ... | 2013 | 23548274 |
| [qualitative composition of dominating forms of microorganisms isolated from radionuclide contaminated soil and their ability to accumulate 137cs]. | qualitative composition of the dominating forms of microorganisms isolated from radionuclide contaminated soils has been studied. the ability to accumulate 137cs by freshly isolated species and collection cultures that were not adapted to the presence of the radionuclide has been analyzed. it is shown that among the analyzed microorganisms the greatest ability to accumulate the radionuclide is inherent in the collection culture bacillus megaterium ukmv-5724. | 2013 | 23516838 |
| preparation and evaluation of bacillus megaterium-alginate microcapsules for control of rice sheath blight disease. | bacillus megaterium encapsulated in calcium alginate microcapsules was prepared and tested for its efficacy against sheath blight disease of rice. in laboratory conditions, the aqueous suspension (1:100, v/v in potato dextrose agar) of the bacterial microcapsules (10(10) spores/ml) inhibited mycelial growth of rhizoctonia solani (>99 %) after the microcapsules were produced and stored for 12 months at room temperature (28 ± 2 °c). the survival of the bacterium in the microcapsules in response to ... | 2013 | 23508397 |
| bioactive polyoxygenated steroids from the south china sea soft coral, sarcophyton sp. | seven new polyoxygenated steroids (1-7) were isolated together with seven known analogues (8-14) from the south china sea soft coral, sarcophyton sp. the structures of the new compounds were identified on the basis of extensive spectroscopic analysis and comparison with reported data. all the steroids are characterized with 3β,5α,6β-hydroxy moiety, displaying carbon skeletons of cholestane, ergostane, gorgostane and 23,24-dimethyl cholestane. in the in vitro bioassay, metabolites exhibited diffe ... | 2013 | 23478486 |
| reconstruction and analysis of the industrial strain bacillus megaterium wsh002 genome-scale in silico metabolic model. | a genome-scale metabolic model of bacillus megaterium wsh002, an industrial bacterium widely used in the vitamin c industry, was reconstructed on the basis of the genome annotation and data from the literature and biochemical databases. it comprises 1112 reactions, 993 metabolites, and 1055 genes, including 43 new annotated genes. this model was able to predict qualitatively and quantitatively the growth of b. megaterium on a range of carbon and nitrogen sources, and the results agreed well with ... | 2013 | 23454894 |
| valorization of glycerol through the production of biopolymers: the phb case using bacillus megaterium. | in this work technical and economic analyses were performed to evaluate the glycerol transformation into polyhydroxybutyrate using bacillus megaterium. the production of phb was compared using glycerol or glucose as substrates and similar yields were obtained. the total production costs for phb generation with both substrates were estimated at an industrial scale. compared to glucose, glycerol showed a 10% and 20% decrease in the phb production costs using two different separation schemes respec ... | 2013 | 23428814 |
| peroxidase activity stabilization of cytochrome p450(bm3) by rational analysis of intramolecular electron transfer. | combined quantum mechanical and molecular mechanical (qm/mm) calculations were used to explore the electron pathway involved in the suicide inactivation of cytochrome p450bm3 from bacillus megaterium. the suicide inactivation is a common phenomenon observed for heme peroxidases, in which the enzyme is inactivated as a result of self-oxidation mediated by highly oxidizing enzyme intermediates formed during the catalytic cycle. the selected model was a mutant comprising only the heme domain (cypbm ... | 2013 | 23425936 |
| glutathione enhances 2-keto-l-gulonic acid production based on ketogulonicigenium vulgare model iwz663. | ketogulonicigenium vulgare is used widely during the industrial production of 2-keto-l-gulonic acid (2-klg), the precursor of vitamin c, in a coculture with bacillus megaterium. we analyzed the sulfate and coenzyme a metabolic module in the genome-scale metabolic model (gsmm) iwz663 and found that the poor growth of k. vulgare was due to a deficiency in key reductases in the sulfate metabolic pathway. to carefully investigate the metabolism of sulfate, we developed a chemically defined medium (c ... | 2013 | 23376843 |
| high production of poly(3-hydroxybutyrate) from a wild bacillus megaterium bolivian strain. | taking into account that a novel strain of bacillus megaterium was isolated from uyuni salt lake (bolivia) in a previous work, the objectives of this new study were to determine the maximal poly-3-hydroxybutyrate production potential of b. megaterium strain uyuni s29 in an industrial conventional media, the possibility that the strain accumulates different types of polyhydroxyalkanoates, the cellular morphology during the biosynthesis process and the characterization of the produced biopolymers. | 2013 | 23360297 |
| enhancing polyhydroxybutyrate production from high cell density fed-batch fermentation of bacillus megaterium ba-019. | this study demonstrated the improved polyhydroxybutyrate (phb) production via high cell density cultivation of bacillus megaterium ba-019 with balanced initial total sugar concentration and carbon to nitrogen (c/n) weight ratio. in the 10 l stirred fermentor operated at 30 °c, ph 7.0, 600 rpm, and 1.0 vvm air, with the initial total sugar concentration of 60 g/l and urea at the c/n weight ratio of 10:1, 32.48 g/l cell biomass with the corresponding phb weight content of 26.94 % and volumetric pr ... | 2013 | 23340799 |
| characterization of polyhydroxyalkanoates accumulated by a moderately halophilic salt pan isolate bacillus megaterium strain h16. | characterization of polyhydroxyalkanoates (pha) accumulated by halophilic bacteria isolated from solar salterns. | 2013 | 23311612 |
| influencing the monophenolase/diphenolase activity ratio in tyrosinase. | tyrosinase is a type 3 copper enzyme with great potential for production of commercially valuable diphenols from monophenols. however, the use of tyrosinase is limited by its further oxidation of diphenols to quinones. we recently determined the structure of the bacillus megaterium tyrosinase revealing a residue, v218, which we proposed to take part in positioning of substrates within the active site. in the structure of catechol oxidase from ipomoea batatas, the lack of monophenolase activity w ... | 2013 | 23305929 |
| structural and stereochemical studies of hydroxyanthraquinone derivatives from the endophytic fungus coniothyrium sp. | four known hydroxyanthraquinones (1-4) together with four new derivatives having a tetralone moiety, namely coniothyrinones a-d (5-8), were isolated from the culture of coniothyrium sp., an endophytic fungus isolated from salsola oppostifolia from gomera in the canary islands. the structures of the new compounds were elucidated by detailed spectroscopic analysis and comparison with reported data. the absolute configurations of coniothyrinones a (5), b (6), and d (8) were determined by tddft calc ... | 2013 | 23255384 |
| application of a new versatile electron transfer system for cytochrome p450-based escherichia coli whole-cell bioconversions. | cytochromes p450 monooxygenases are highly interesting biocatalysts for biotechnological applications, since they perform a diversity of reactions on a broad range of organic molecules. nevertheless, the application of cytochromes p450 is limited compared to other enzymes mainly because of the necessity of a functional redox chain to transfer electrons from nad(p)h to the monooxygenase. in this study, we established a novel robust redox chain based on adrenodoxin, which can deliver electrons to ... | 2013 | 23254762 |
| high yield production of extracellular recombinant levansucrase by bacillus megaterium. | in this study, a high yield production bioprocess with recombinant bacillus megaterium for the production of the extracellular enzyme levansucrase (sacb) was developed. for basic optimization of culture parameters and nutrients, a recombinant b. megaterium reporter strain that produced green fluorescent protein under control of a vector-based xylose-inducible promoter was used. it enabled efficient microtiter plate-based screening via fluorescence analysis. a ph value of ph 6, 20 % of dissolved ... | 2013 | 23179620 |
| [molecular mechanism of co-culturing bacillus megaterium and ketogulonigenium vulgare--a review]. | co-culturing bacillus megaterium and ketogulonigenium vulgare is widely applied to 2-keto-gulonic acid production. for optimizing the process, numerous researchers studied on the symbiotic molecular mechanism of the co-culture process. the research was promoted greatly owing to omics technologies, bioinformatics, high throughput technologies and physiology. recently, the proteomic, metabolomic, comparative genomics and transcriptomics were performed to the research. these omics data provided us ... | 2012 | 23173429 |
| characterization of the enzyme cbih60 involved in anaerobic ring contraction of the cobalamin (vitamin b12) biosynthetic pathway. | the anaerobic pathway for the biosynthesis of cobalamin (vitamin b(12)) has remained poorly characterized because of the sensitivity of the pathway intermediates to oxygen and the low activity of enzymes. one of the major bottlenecks in the anaerobic pathway is the ring contraction step, which has not been observed previously with a purified enzyme system. the gram-positive aerobic bacterium bacillus megaterium has a complete anaerobic pathway that contains an unusual ring contraction enzyme, cb ... | 2013 | 23155054 |
| efficacy of using harmless bacillus endospores to estimate the inactivation of cryptosporidium parvum oocysts in water. | the need to use complex in vitro cell culture, expensive equipment, and highly-trained technicians that are available only to specialist laboratories has significantly limited studies assessing the potential of pulsed uv light (puv) to inactivate the waterborne parasite cryptosporidium parvum in drinking water. this constitutes the first study to report on the use of different non-pathogenic bacillus endospores as potential surrogate organisms to indicate the puv inactivation performance of a c. ... | 2013 | 23145570 |
| essential oil variation in the populations of artemisia spicigera from northwest of iran: chemical composition and antibacterial activity. | artemisia spicigera c. koch (asteraceae) is a perennial shrubby herb and is generally distributed in armenia, iran, and middle anatolia. this species traditionally has been used in medicines. | 2013 | 23126238 |
| genomic sequence of heliothis virescens ascovirus 3g isolated from spodoptera exigua. | heliothis virescens ascovirus 3a (hvav-3a), a member of the family ascoviridae, has the highest diversity among ascovirus species that have been reported in australia, indonesia, china, and the united states. to understand the diversity and origin of this important ascovirus, the complete genome of the hvav indonesia strain (hvav-3g), isolated from spodoptera exigua, was determined to be 199,721 bp, with a g+c content of 45.9%. therefore, hvav-3g has the largest genome among the reported ascovir ... | 2012 | 23087124 |
| a novel integrated approach to quantitatively evaluate the efficiency of extracellular polymeric substances (eps) extraction process. | a novel integrated approach is developed to quantitatively evaluate the extracellular polymeric substances (eps) extraction efficiency after taking into account eps yield, eps damage, and cell lysis. this approach incorporates grey relational analysis and fuzzy logic analysis, in which the evaluation procedure is established on the basis of grey relational coefficients generation, membership functions construction, and fuzzy rules description. the flocculation activity and dna content of eps are ... | 2012 | 23064456 |
| overexpression of a paenibacillus campinasensis xylanase in bacillus megaterium and its applications to biobleaching of cotton stalk pulp and saccharification of recycled paper sludge. | a xylanase gene (xyng1-1) from paenibacillus campinasensis g1-1 was expressed in bacillus megaterium ms941 and a high level of extracellular xylansae activity (304.26 iu/ml) was achieved after induction with 0.5% xylose. the purified recombinant xylanase (xyng1-1r) revealed optimal activity at 60°c and ph 7.0 and retained 79% and 81% activity after incubation without substrate at 60°c, ph 5.0 and ph 8.0 for 3h, respectively. application of xyng1-1r (15 iu/g pulp) to cotton stalk pulp bleaching i ... | 2012 | 23026332 |
| production of α-cyclodextrin glycosyltransferase in bacillus megaterium ms941 by systematic codon usage optimization. | α-cyclodextrin glycosyltransferase is a key enzyme in the cyclodextrin industry. the gram-positive bacterium bacillus megaterium was chosen for production of recombinant α-cgtase for safety concerns. successful production of heterologous α-cgtase was achieved by adapting the original α-cgt gene to the codon usage of b. megaterium by systematic codon optimization. this balanced the trna pool and reduced ribosomal traffic jams. protein expression and secretion was ensured by using the strong induc ... | 2012 | 23013320 |
| superstructure of the centromeric complex of tubzrc plasmid partitioning systems. | bacterial plasmid partitioning systems segregate plasmids into each daughter cell. in the well-understood parmrc plasmid partitioning system, adapter protein parr binds to centromere parc, forming a helix around which the dna is externally wrapped. this complex stabilizes the growth of a filament of actin-like parm protein, which pushes the plasmids to the poles. the tubzrc plasmid partitioning system consists of two proteins, tubulin-like tubz and tubr, and a dna centromere, tubc, which perform ... | 2012 | 23010931 |
| structure, mechanism and regulation of an artificial microbial ecosystem for vitamin c production. | at present, the modern two-step fermentation process is one of the major approaches for the industrial production of vitamin c. the key step in this process is the conversion of l-sorbose to 2-keto-l-gulonic acid (2-klg), the vitamin c precursor, which is accomplished by an artificial microbial ecosystem consisting of ketogulonicigenium vulgare and bacillus megaterium. this review describes current progress in understanding this ecosystem, not only the individual physiological characteristics of ... | 2013 | 22994289 |
| discontinuous and continuous purification of single-chain antibody fragments using immobilized metal ion affinity chromatography. | this work describes the adsorption-desorption behavior of a histidine-tagged single-chain fragment variable antibody (d1.3 scfv) on a commercial immobilized metal ion affinity chromatography (imac) column. a clarified cell culture supernatant originating from bacillus megaterium was characterized using single column experiments in a ph-gradient elution mode. the cell culture supernatant containing the antibody fragment d1.3 scfv could be treated in the chromatographic separation process as a pse ... | 2013 | 22985797 |
| a rapid screening for cytochrome p450 catalysis on new chemical entities: cytochrome p450 bm3 and 1,2,5-oxadiazole derivatives. | this work presents the validation of a rapid screening procedure for the catalysis of cytochrome p450 on new chemical entities. the assay is tested on the prototypical, catalytically self-sufficient and soluble cytochrome p450 bm3 from bacillus megaterium that shares a high degree of homology with mammalian counterparts. the so-called alkali assay developed in our laboratory is validated here also by product formation and molecular modeling on a number of derivatives sharing the molecular scaffo ... | 2013 | 22983164 |
| alteration of the mode of antibacterial action of a defensin by the amino-terminal loop substitution. | ancient invertebrate-type and classical insect-type defensins (aitds and citds) are two groups of evolutionarily related antimicrobial peptides (amps) that adopt a conserved cysteine-stabilized α-helical and β-sheet (csαβ) fold with a different amino-terminal loop (n-loop) size and diverse modes of antibacterial action. although they both are identified as inhibitors of cell wall biosynthesis, only citds evolved membrane disruptive ability by peptide oligomerization to form pores. to understand ... | 2012 | 22975352 |
| microbiological stabilization of aloe vera (aloe barbadensis miller) gel by high hydrostatic pressure treatment. | the effect of high hydrostatic pressure (hhp) treatment (300, 400 and 500 mpa for 1 and 3 min at 20 °c) on the microbiological shelf-life and microbiota composition of aloe vera gel during 90 days of storage at 4 °c was investigated. aerobic mesophilic and psychrotrophic bacteria, as well as moulds and yeasts, were enumerated after hhp treatment and through cold storage. randomly selected isolates from the count plates were identified by standard methods and the api identification system. result ... | 2012 | 22884172 |
| engineering macaca fascicularis cytochrome p450 2c20 to reduce animal testing for new drugs. | in order to develop in vitro methods as an alternative to p450 animal testing in the drug discovery process, two main requisites are necessary: 1) gathering of data on animal homologues of the human p450 enzymes, currently very limited, and 2) bypassing the requirement for both the p450 reductase and the expensive cofactor nadph. in this work, p450 2c20 from macaca fascicularis, homologue of the human p450 2c8 has been taken as a model system to develop such an alternative in vitro method by two ... | 2012 | 22819650 |
| structure-guided mutagenesis for the improvement of substrate specificity of bacillus megaterium glucose 1-dehydrogenase iv. | bacillus megaterium iam 1030 (bacillus sp. jcm 20016) possesses four d-glucose 1-dehydrogenase isozymes (bmglcdh-i, -ii, -iii and -iv) that belong to the short-chain dehydrogenase/reductase superfamily. the bmglcdhs are currently used for a clinical assay to examine blood glucose levels. of these four isozymes, bmglcdh-iv has relatively high thermostability and catalytic activity, but the disadvantage of its broad substrate specificity remains to be overcome. here, we describe the crystal struct ... | 2012 | 22804868 |
| [bacterial biodiversity in dongcai, a traditional pickled mustard product in sichuan province, china]. | to investigate the bacteria community and biodiversity of four-years pickled yanshan dongcai. | 2012 | 22799218 |
| [reconstruction and analysis of bacillus megaterium metabolic model based on literature study]. | the aim of this study is to reconstruct the metabolic model of bacillus megaterium by literature study, which would be used to elucidate physiological properties in detail and refine physiological functions. | 2012 | 22799211 |
| capability of a selected bacterial consortium for degrading diesel/biodiesel blends (b20): enzyme and biosurfactant production. | the search for alternative sources of energy, such as biodiesel, has been stimulated, since this biofuel is highly susceptible for biodegradation and has low toxicity, thus, reducing the impact in ecosystems. the objective of this study was to select a bacterial consortium with potential for degrading diesel/biodiesel blends (b20) obtained from areas contaminated with hydrocarbons/esters. in order to evaluate the biodegrability of the blend, six enzyme assays were conducted: alkane hydroxylase, ... | 2012 | 22755524 |
| getting the big beast to work--systems biotechnology of bacillus megaterium for novel high-value proteins. | the high industrial relevance of the soil bacterium bacillus megaterium as host for recombinant proteins is driving systems-wide analyses of its metabolic and regulatory networks. the present review highlights novel systems biology tools available to unravel the various cellular components on the level of metabolic and regulatory networks. these provide a rational platform for systems metabolic engineering of b. megaterium. in line, a number of interesting studies have particularly focused on st ... | 2013 | 22750448 |
| optimizing of the formation of active bmw-amylase after in vitro refolding. | this study was carried out to determine the optimal folding condition of α-amylase from bacillus megaterium who using response surface methodology (rsm). a first-order model showed that three factors namely glycerol, ca(2+) and protein concentration had the most significant effect on refolding. analysis of the results showed that glycerol was better than the other polyols due to its effect on protein stability. since α-amylases are known to contain calcium ions in their structure, the presence o ... | 2012 | 22750395 |
| effect of ribose, xylose, aspartic acid, glutamine and nicotinic acid on ethyl (s)-4-chloro-3-hydroxybutanoate synthesis by recombinant escherichia coli. | most reductases which belong to the short chain dehydrogenase/reductase (sdr) superfamily require nad (p) h for activity. addition of this cofactor was still necessary for the production of ethyl (s)-4-chloro-3-hydroxybutanoate by escherichia coli even when a cofactor regeneration system was constructed by co-expressing carbonyl reductase from pichia stipitis (pscri) and glucose dehydrogenase from bacillus megaterium (bmgdh). in an attempt to reduce dependence on the expensive cofactor, compound ... | 2012 | 22698447 |
| [antimicrobial activity of bacillus megaterium strains]. | | 2012 | 22693828 |
| characterization of mycolytic enzymes of bacillus strains and their bio-protection role against rhizoctonia solani in tomato. | four antagonists bacteria namely, bacillus megaterium mb3, b. subtilis mb14, b. subtilis mb99 and b. amyloliquefaciens mb101 were able to produce chitinase, β-1,3-glucanase and protease in different range with the presence of rhizoctonia solani cell wall as a carbon source. amplification of chitinase (chia) gene of 270 bp and β-1, 3-glucanase gene of 415 bp was given supportive evidence at molecular level of antibiosis. after in vitro screening, all antagonists were tested against r. solani unde ... | 2012 | 22684745 |
| the anaerobic biosynthesis of vitamin b12. | vitamin b12 (cobalamin) is a cobalt-containing modified tetrapyrrole that is an essential nutrient for higher animals. its biosynthesis is restricted to certain bacteria and requires approximately 30 enzymatic steps for its complete de novo construction. remarkably, two distinct biosynthetic pathways exist, which are termed the aerobic and anaerobic routes. the anaerobic pathway has yet to be fully characterized due to the inherent instability of its oxygen-sensitive intermediates. bacillus mega ... | 2012 | 22616870 |
| a chimeric toxin vaccine protects against primary and recurrent clostridium difficile infection. | the global emergence of clostridium difficile infection (cdi) has contributed to the recent surge in severe antibiotic-associated diarrhea and colonic inflammation. c. difficile produces two homologous glucosylating exotoxins, tcda and tcdb, both of which are pathogenic and require neutralization to prevent disease occurrence. however, because of their large size and complex multifunctional domain structures, it has been a challenge to produce native recombinant toxins that may serve as vaccine ... | 2012 | 22615245 |
| isolation and characterization of feather degrading enzymes from bacillus megaterium sn1 isolated from ghazipur poultry waste site. | the sn1 strain of bacillus megaterium, isolated from soil of ghazipur poultry waste site (india) produced extracellular caseinolytic and keratinolytic enzymes in basal media at 30 degrees c, 160 rpm in the presence of 10% feather. feathers were completely degraded after 72 h of incubation. the caesinolytic enzyme was separated from the basal media following ammonium sulphate precipitation and ion exchange chromatography. we report 29.3-fold purification of protease after q sepharose chromatograp ... | 2013 | 22586913 |
| improvement of thermostability and activity of pectate lyase in the presence of hydroxyapatite nanoparticles. | the activity and half-life of pectate lyase (pl) from bacillus megaterium were nine- and 60-fold, respectively, higher at 90 °c in the presence of hydroxyapatite nanoparticles (np-pls) than in the presence of 1mm cacl(2). thermodynamic analysis of the nanoparticle-induced stability revealed an enhanced entropy-enthalpy compensation by the np-pls since a reciprocal linearity of the enthalpy-entropy change to 90 °c was observed. without nanoparticles, the linearity range was 70 °c. such compensati ... | 2012 | 22541951 |
| changes in tyrosinase specificity by ionic liquids and sodium dodecyl sulfate. | tyrosinase is a member of the type 3 copper enzyme family involved in the production of melanin in a wide range of organisms. the ability of tyrosinases to convert monophenols into diphenols has stimulated studies regarding the production of substituted catechols, important intermediates for the synthesis of pharmaceuticals, agrochemicals, polymerization inhibitors, and antioxidants. despite its enormous potential, the use of tyrosinases for catechol synthesis has been limited due to the low mon ... | 2013 | 22539021 |
| comparative structural modeling and docking studies of uricase: possible implication in enzyme supplementation therapy for hyperuricemic disorders. | uricase (ec 1.7.3.3, uc) catalyzes the oxidation of uric acid (ua) to more soluble allantoin thereby lowering plasma ua levels. in humans, when concentration of ua exceeds >7mg/dl, it leads to hyperuricemia, gout, nephrolithiasis and urolithiasis. a new remedy to cure such metabolic diseases is the enzyme supplementation therapy by uc but with high degree of antigenic independence. therefore screening of new uricase sources to expand its usefulness and reduced antigenecity is needed. present stu ... | 2012 | 22537975 |
| spatial configuration of extracellular polymeric substances of bacillus megaterium tf10 in aqueous solution. | configuration of extracellular polymeric substances (eps) excreted by microorganisms is related greatly to the inherent properties of eps, and has a significant effect on the physicochemical characteristics of microbial aggregates, such as activated sludge for wastewater treatment, as well as their interaction with other substances in aqueous systems. in this work, the spatial configuration of microbial eps is characterized using laser light scattering (lls) technique, with eps extracted from ba ... | 2012 | 22507252 |
| the biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles. | spherical 3.5 nm diameter silver nanoparticles (agnp) stabilized in type i collagen (agnp@collagen) were prepared in minutes (5-15 min) at room temperature by a photochemical method initiated by uva irradiation of a water-soluble non-toxic benzoin. this biocomposite was examined to evaluate its biocompatibility and its anti-bacterial properties and showed remarkable properties. thus, while keratinocytes and fibroblasts were not affected by agnp@collagen, it was bactericidal against bacillus mega ... | 2012 | 22494887 |
| novel probiotic candidates for humans isolated from raw fruits and vegetables. | this study was aimed at determining the probiotic potential of a large number of autochthonous lactic acid bacteria isolated from fruit and vegetables. survival under simulated gastric and intestinal conditions showed that 35% of the strains, mainly belonging to the species lactobacillus plantarum maintained high cell densities. selected strains did not affect the immune-mediation by caco-2 cells. all strains stimulated all 27 immune-mediators by peripheral blood mononuclear cells (pbmc). a sign ... | 2012 | 22475949 |
| application of phosphate-solubilizing bacteria for enhancing bioavailability and phytoextraction of cadmium (cd) from polluted soil. | in this study, phosphate-solubilizing bacteria (psb), bacillus megaterium, were used to enhance cd bioavailability and phytoextractability of cd from contaminated soils. this strain showed a potential for directly solubilizing phosphorous from soils more than 10 folds greater than the control without inoculation. the results of pot experiments revealed that inoculation with b. megaterium significantly increased the extent of cd accumulation in brassica juncea and abutilon theophrasti by two fold ... | 2012 | 22472099 |