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reaction of anti-hla-b monoclonal antibodies with envelope proteins of shigella species. evidence for molecular mimicry in the spondyloarthropathies.immunologic cross-reactivity between enteric bacteria and the hla-b27 protein may play a role in the etiology of reiter's syndrome and reactive arthritis. the reactivity of two anti-b27 mab (b27m1 and b27m2) with envelope proteins of shigella flexneri isolated from reiter's syndrome patients was studied by western blot analysis. proteins with an apparent mr of approximately 36 and 23 kda reacted with both mab in ascites. mab against related hla class i ag b7 and b40 did not react with the 23 kda ...19883283236
serum antibodies to lipopolysaccharide and natural immunity to shigellosis in an israeli military population. 19883283258
conservation of an atp-binding domain among reca proteins from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli k-12 and b/r.the purified reca proteins encoded by the cloned genes from proteus vulgaris, erwinia carotovora, shigella flexneri, and escherichia coli b/r were compared with the reca protein from e. coli k-12. each of the proteins hydrolyzed atp in the presence of single-stranded dna, and each was covalently modified with the photoaffinity atp analog 8-azidoadenosine 5'-triphosphate (8n3atp). two-dimensional tryptic maps of the four heterologous reca proteins demonstrated considerable structural conservation ...19883286605
[clinico-immunological characteristics of mixed intestinal infections in children]. 19883287323
[changes in the degree of sensitivity to antibiotics of shigella isolated from patients with dysentery in a city infectious disease hospital over the past 8 years].changes in sensitivity of shigella and in particular shigella flexneri isolated from dysentery patients in a city infection hospital within 8 years i.e. from 1977 to 1985 were followed. it was shown that the changes were to the greater extent connected with preferable use of certain antibiotics in the hospital.19883288150
suggestion to supplement shigella flexneri classification scheme with the subserovar shigella flexneri 4c: phenotypic characteristics of strains.a new serovar of shigella flexneri has been isolated with increasing frequency in the ussr. it has the antigenic formula iv:7,8. we suggest that it be incorporated into the classification scheme as s. flexneri subserovar 4c. the 212 strains of this proposed subserovar examined to date display biochemical and serological properties typical of the species, are sensitive to the genus-specific bacteriophage, and cause keratoconjunctivitis in the sereny test.19883290245
salmonellal mycotic abdominal-aortic aneurysm.a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...19883292882
salmonellal mycotic abdominal-aortic aneurysm.a patient is described with a mycotic abdominal-aortic aneurysm that was caused by salmonella typhimurium, who presented to hospital four months after a urinary-tract infection that was caused by the same organism. the urinary-tract infection may have resulted from an episode of transient salmonellal bacteraemia, the significance of which was not appreciated. the pathogenesis, clinical features, treatment and prognosis of arteritis due to salmonella species are reviewed. the early diagnosis and ...19883292882
demetrio sodi-pallares: the man and his thought. 19883293862
effects of marine mammal parasites on human health. 19873294653
molecular cloning of invasion plasmid antigen (ipa) genes from shigella flexneri: analysis of ipa gene products and genetic mapping.tn5-tagged invasion plasmid dna (pwr110) from shigella flexneri serotype 5 (strain m90t) was cloned into the expression vector lambda gt11. recombinant phage (lambda gt11sfl) expressing pwr110-encoded polypeptide antigens were identified by using rabbit antisera directed against s. flexneri m90t invasion plasmid antigens. antigens encoded by lambda gt11sfl recombinant phage were characterized by reacting affinity-purified antibodies, eluted from nitrocellulose-bound plaques of lambda gt11sfl rec ...19873294797
an aminoacyl-trna synthetase complex in escherichia coli.aminoacyl-trna synthetases from several strains of escherichia coli are shown to elute as a high-molecular-weight complex on 6% agarose columns (bio-gel a-5m). in contrast, very little synthetase activity was observed in such complexes on sephadex g-200 columns, suggesting that these enzymes may interact with or are dissociated during chromatography on dextran. the size of the complex observed on bio-gel a-5m was influenced by the method of cell breakage and the salt concentrations present in bu ...19873294804
an accessory protein identical to mouse egasyn is complexed with rat microsomal beta-glucuronidase and is identical to rat esterase-3.we report biochemical, immunological, and genetic studies which demonstrate that an accessory protein with the essential features of mouse egasyn is complexed with and stabilizes a portion of beta-glucuronidase in microsomes of rat liver. the accessory protein exists as a complex with beta-glucuronidase since it coprecipitates with beta-glucuronidase after treatment of extracts with a specific beta-glucuronidase antibody. the two proteins are associated by noncovalent bonds since they are easily ...19873294829
bacterial invasion of fibroblasts induces interferon production.although bacteria and/or bacterial products have been shown to induce interferon (ifn) production in cells of the immune system, the ability of non-lymphoid cells to produce ifn in response to bacterial products and/or bacterial invasion is currently unknown. in this study we examined murine and human fibroblasts for their ability to produce ifn in response to challenge with the invasive bacteria shigella flexneri and enteroinvasive escherichia coli. both human and murine primary cell cultures p ...19873295042
toxic megacolon complicating shigellosis. 19873295219
[dynamics of bacterial polysensitization in acute dysentery in children depending on the species of the causative agent].studies on polysensitization, evaluated by the neutrophil damage index, have been made with the use of dysenterin and the allergens of hemolytic staphylococci, enterococci and escherichia coli. the studies have revealed that both specific and nonspecific sensitization to hemolytic staphylococci and enterococci is higher in dysentery caused by shigella flexneri than in dysentery caused by s. sonnei and has the tendency to increase in the course of the disease.19873296580
[gardnerella vaginalis in nonspecific vaginitis]. 19873296818
humoral response in mice immunized with outer membrane proteins of shigella flexneri.intraperitoneal immunization of mice with outer membrane proteins (omp) of sh. flexneri induced in the animals a synthesis of specific antibodies. their level determined by elisa test was found to be relatively low in the sera of animals immunized with a single dose (10 micrograms) of omp; it was markedly higher in mice immunized with two doses of omp, and very high after three fold immunization. the specific antibodies maintained in the animals for 8-16 weeks after immunization. anti-omp sera g ...19863296993
epinephrine, glucose, and lactate infusion in exercising adrenodemedullated rats.the purpose of this study was to determine the metabolic function of the marked increase in plasma epinephrine which occurs in fasted rats during treadmill exercise. fasted adrenodemedullated (adm) and sham-operated (sham) rats were run on a rodent treadmill (21 m/min, 15% grade) for 30 min or until exhaustion. adm rats were infused with saline, epinephrine, glucose, or lactate during the exercise bouts. adm saline-infused rats showed markedly reduced endurance, hypoglycemia, elevated plasma ins ...19873298192
the protein subunits of the double-stranded rna dependent rna polymerase and methyltransferase of the cytoplasmic polyhedrosis virus of silkworm, bombyx mori.the particles of cpv of silkworm contain double-stranded rna polymerase and methyltransferase. it was reported in a previous paper that the genome-enzyme complex could be isolated. the genome-enzyme complex shows high enzyme activity of rna polymerase and methyltransferase in spite of the fact that it consists of only 5 percent of the protein. in order to clarify the protein subunits of the rna polymerase and methyltransferase, two methods were adopted. the sds-polyacrylamide gel electrophoretog ...19863299695
erg, a human ets-related gene on chromosome 21: alternative splicing, polyadenylation, and translation.the avian acute leukemia virus e26 induces a mixed erythroid-myeloid leukemia in chickens and carries two distinct oncogenes, v-myb and v-ets. recently, a novel gene named erg, closely related to the v-ets oncogene, was identified in human colo 320 cells and the nucleotide sequence of its approximately 5.0-kilobase transcript, erg 1 was determined. in the present study, the nucleotide sequence of the alternatively spliced transcript, erg 2, was found to differ from erg 1 by a splicing event that ...19873299708
[growth and development of a population of the causative agent during an infection]. 19873299739
multiple enteric pathogens in a patient with diarrhoea.a woman who recently arrived in canada from india developed a febrile, diarrhoeal illness. stool culture revealed the presence of salmonella typhi, campylobacter fetus subspecies jejuni and shigella flexneri. concurrent infection with these three pathogens does not appear to have been previously reported. the possibility of multiple pathogens should be considered in travellers who fail to respond to treatment of a single organism.19873299934
corneal ulcer caused by shigella flexneri in an infant. 19873300355
live dysentery vaccine "vadizen" (t32-istrati) immunogenicity against shigella-like challenge in animals. 19863300598
ultrastructural study on the bacterial symbiont in giardia lamblia. 19863300600
[reye-like syndrome due to shigella flexneri in a child]. 19873301584
treatment of bacillary dysentery: a comparison between enoxacin and nalidixic acid.a prospective randomized study has been conducted in rwanda to compare the efficacy of enoxacin versus nalidixic acid for the treatment of bacillary dysentery in adults. seventeen patients received nalidixic acid and 21 received enoxacin. all isolates (37 shigella, 1 campylobacter) were sensitive in vitro to the two drugs. all patients showed a favourable clinical and bacteriological response and the results obtained with both drugs were comparable. enoxacin seems to be a good alternative to nal ...19873301783
clinical presentation of shigellosis during the 1984 epidemic of bacillary dysentery in west bengal. 19873301795
shigellosis in bangladesh: a reply. 19873302055
retardation of cell cycle progression in yeast cells recovering from dna damage: a study at the single cell level.pedigree analyses of individual yeast cells recovering from dna damage were performed and time intervals between morphological landmark events during the cell cycle (bud emergence and cell separation), were recorded for three generations. the associated nuclear behavior was monitored with the aid of dapi staining. the following observations were made: all agents tested (x-rays, mms, ems, mnng, nitrous acid) delayed the first bud emergence after treatment, which indicates inhibition of the initia ...19873302599
production of monospecific antibodies to a low-abundance hepatic membrane protein using nitrocellulose immobilized protein as antigen.membrane proteins from primary cultures of rat hepatocytes were separated by two-dimensional polyacrylamide gel electrophoresis. the proteins were transferred to nitrocellulose paper which was then dissolved in dimethyl sulfoxide and this mixture was used as a primary immunogen in rabbits. subsequent immunizations were performed using nonsolubilized protein immobilized on nitrocellulose paper. a monospecific polyclonal antibody was generated against a specific mitochondrial membrane protein (mp- ...19873304007
molecular cloning and expression of a synthetic dna coding for the antimicrobial protein of bull seminal plasma.a dna carrying the coding sequence for the antimicrobial protein from bull seminal plasma (sap) was obtained by enzymic ligation of six synthetic oligonucleotides. the 162 bp synthetic dna fragment was cloned into the c-terminal part of the lacz-gene employing the vector pur289. expression in e. coli in the presence of the inducer isopropylthiogalactoside (iptg) led to the formation of a fusion protein, which was shown by immuno-blotting to contain immuno-reactive antimicrobial protein. approxim ...19873304335
[reaction of the hematopoietic system in mice to the administration of virulent and avirulent strains of shigella flexneri 2a].the influence of live bacteria and antigenic preparations of virulent and avirulent stains of shigella flexneri 2a on the endogenous splenic colony formation in murine hematopoietic cells has been studied. the different stimulating activity of live microbial cells of virulent and avirulent strains shigella flexneri 2a and their antigenic preparations on the endogenous colony formation has been shown. the effect observed depends on the preparation doses and time of their administration before irr ...19873304434
a new chromogenic test for the detection of urokinase in the genus shigella.a simple test for detection of urokinase in shigella is described. the test is performed by suspending a loopful of bacteria in 100 microliters of a buffered 1mm solution of benzoyl-beta-alanyl glycyl-arginyl-4-nitroanilide acetate (chromozym u). enzymatic activity is revealed by formation of a yellow colour after 24 h of incubation at 37 degrees c. the test is able to differentiate serotypes of shigella dysenteriae, shigella flexneri, shigella boydii. the results suggest the possibility of incl ...19873306294
chronobiology and epidemiology.epidemiology and chronobiology are two emerging scientific disciplines of the 20th century. in the united states and certain european and asian countries, epidemiology is a well-developed science; however, in many countries the field is not yet well practiced. too, in several european countries and certain centers in the united states, chronobiology is quite well known and respected as a science. yet, in many countries this field is just now being recognized. although epidemiologists are concern ...19873306581
[morphological changes in the lungs after single whole-body irradiation in rabbits].morphological changes in rabbit lungs were studied after whole-body nonuniform irradiation (24 gy) the head and the abdomen being shielded. acute pneumonitis was shown to develop between the 17th and the 24th day after irradiation. no pulmonary fibrosis was registered 60 days following irradiation. embolised megakaryocytes were found in the lung capillaries between the 8th and the 24th day after irradiation.19873306764
atrio-ventricular block associated with shigella flexneri infection. 19873311739
entry of shigella flexneri into hela cells: evidence for directed phagocytosis involving actin polymerization and myosin accumulation.the enteroinvasive bacterium shigella flexneri expresses a plasmid-mediated capacity to penetrate into nonphagocytic cells. by using 7-nitrobenz-2-oxa-1,3-diazole-phallacidin (nbd-phallacidin), a fluorescent dye which specifically stains microfilaments, we observed condensations of filamentous actin underneath the plasma membrane of hela cells which interacted with the invasive isolate m90t. as demonstrated by indirect immunofluorescence with the antimyosin monoclonal antibody cc-212, myosin acc ...19873312007
introduction of shigella flexneri 2a type and group antigen genes into oral typhoid vaccine strain salmonella typhi ty21a.for protection against dysentery caused by shigella flexneri 2a, an in vivo-constructed recombinant plasmid with genes specifying the s. flexneri type and group antigens located near the pro (min 6) and his (min 44) chromosomal markers, respectively, was made and transferred to the gale salmonella typhi strain ty21a. strain ty21a carrying this recombinant plasmid was shown by immunological and biochemical analyses to express the s. flexneri 2a type and group antigens. mice immunized with this va ...19873312013
expression of campylobacter jejuni invasiveness in cell cultures coinfected with other bacteria.enteroinvasive salmonella, shigella, and escherichia coli strains were found to exert an effect which rendered campylobacter jejuni capable of intracellular localization in epithelial cells in vitro. when monolayers of hep-2 or a-549 cells were challenged with pure cultures of c. jejuni or campylobacter coli, none of the eight strains tested invaded the cells. in contrast, four of these strains were able to localize intracellularly when the cells were challenged with a mixture of campylobacters ...19873312015
[bacillary dysentery 1985]. 19873313536
hemolytic uremic syndrome associated with shigellosis: report of two cases.two patients with hemolytic uremic syndrome admitted to udornthani hospital, thailand, were reported. the preceding illness was shigellosis. the stool culture was positive for shigella dysenteriae in one patient and shigella flexneri in another. the management was successful with peritoneal dialysis in one and symptomatic treatment in another. both patients had complete recovery.19873313740
[prospects for using thymalin in treating bacterial intestinal infections]. 19873314133
technological investigations with attenuated strains of shigella for production of live vaccines. 1. cultivation in a fermenter of an attenuated strain s. flexneri 2a with two markers.a method was developed for cultivation of the strain shigella flexneri 2a 77 with two attenuated markers. it ensures the preservation of the initial properties of the strain. the regimen for control of dissolved oxygen level was optimized. it was established that yeast extract is a necessary component of the nutritive medium used. the role of the phase and quantity of the inoculum for growth of the bacterial population has been clarified. fresh fermenter culture, applied in rats, shows a high im ...19873314220
shigella bacteremia in adults. a report of five cases and review of the literature.shigella bacteremia is rare, occurring mainly in children. we describe five adult patients with shigella bacteremia and review data on 22 cases reported in the english-language medical literature. eighteen (67%) of 27 patients had either an underlying condition or were aged older than 65 years. most patients had clinical signs of acute febrile gastroenteritis. however, in six patients, the organism was not isolated from stool. species isolated from blood included shigella flexneri in 11 patients ...19873314766
a 2d-1h-n.m.r. study of some shigella flexneri o-polysaccharides.a 1h-n.m.r. study of the o-polysaccharides from different types of shigella flexneri has been performed. with the aid of 2d-n.m.r. techniques, namely, j-resolved, spin-spin correlation, and noesy experiments, most of the structural features of these polysaccharides could be deduced. sequences could generally be obtained from the noesy experiments. when using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjac ...19873315202
[biological properties of opportunistic microorganisms isolated from patients with acute intestinal diseases].the comparative study of the incidence of the pathogenicity markers (dnaase, rnaase, phosphatase and hemolytic activity) in shigellae and salmonellae, acknowledged as the causative agents of intestinal infections, and in opportunistic bacteria isolated from the feces of patients with acute intestinal diseases and healthy persons has been made. the study has revealed that dnaase and rnaase activity occurs most frequently in shigella flexneri, in salmonellae and in opportunistic enterobacteria iso ...19873318225
shigellosis at a caribbean resort. hamburger and north american origin as risk factors.during a three-week period in 1984, 339 tourists and expatriate employees with diarrhea visited the infirmary at a caribbean resort club. epidemiologic studies suggested that over 60% of the 1,893 tourists at the resort club during that time may have been ill. shigella flexneri 4a was isolated from seven of 18 stool specimens. a few cases of diarrheal illness occurred at the resort club before the onset of the outbreak, which was temporally related to illness in a butcher. north american residen ...19873318410
[medico-genetic studies of human provisional organs]. 19873318529
improved immunohistochemical staining of angiotensin ii in rat brain using affinity purified antibodies.recent immunohistochemical studies that have sought to detect angiotensin ii/iii (aii/aiii) immunoreactive material in the brain have been forced to rely on a small number of antisera because most aii/aiii antibodies have unexplainably proved unsuitable for immunohistochemistry. although extremely useful tools, these antisera have suffered from high background staining. the purpose of this study was to re-examine and characterize the staining using the most popular aii/aiii antiserum (denise) be ...19873319044
pathogenesis of shigella infections. 19873321018
comparative aspects of canine and human atopic dermatitis. 19873321301
novel oral vaccines: the effectiveness of cloned gene products on inducing secretory immune responses. 19873321939
inhibition of enteropathogenic escherichia coli (strain rdec-1) adherence to rabbit intestinal brush borders by milk immune secretory immunoglobulin a. 19873321942
enhancement of the secretory iga response to enteropathogens. 19873321943
plasmid-mediated contact haemolytic activity in shigella species: correlation with penetration into hela cells.the main feature of virulent strains of shigella is their ability to invade eucaryotic cells. this phenotype is both plasmid-mediated and temperature-regulated. in the present report, we demonstrate a plasmid-mediated and temperature-regulated haemolytic activity in s. flexneri, s. dysenteriae and s. sonnei. detection of this haemolytic activity requires centrifugation of suspensions containing bacteria and erythrocytes, followed by incubation of the pellets at 37 degrees c. no soluble intra- or ...19863322171
invasion of epithelial cells by shigellae. 19863322174
plasmid-mediated intracellular multiplication of shigella flexneri. 19863322175
the relevance of controlled fluoride release with bonded orthodontic appliances. 19873323277
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...19873324953
[dependence of the antimicrobial effect on the antibiotic concentration in modelling pharmacokinetic profiles in vitro: a comparison of 2 regimens for the single intravenous administration of sisomicin].kinetics of sisomicin antimicrobial effect on e. coli was studied in an in vitro dynamic model. pharmacokinetic profiles observed in blood of humans treated with different polar dosing schedules i. e. bolus administration and continuous infusion were simulated. the first set of the experiments included simulating the profiles observed at short-term maintenance (0.25-20 min) of relatively high antibiotic levels after bolus administration (cmax = 0.125-10 micrograms/ml). the second set included si ...19873324953
a new keratinolytic proteinase from clinical isolates of trichophyton mentagrophytes. 19873325542
[economic loss from dysentery combined with ascariasis and trichocephaliasis]. 19873325790
ascending urinary tract infections in rats induced by staphylococcus saprophyticus and proteus mirabilis.ascending pyelonephritis was induced by staphylococcus saprophyticus and proteus mirabilis both by inoculating the organisms separately and in combination into rat bladders. bacterial cultures of tissue homogenates showed that pyelonephritis by both bacteria occurred significantly more often in rats where the two organisms were instilled concomitantly, suggesting a synergistic virulence between the two species. the antibody response to the different organisms was the same either the bacteria wer ...19873326399
effect of outer membrane proteins of shigella on delayed hypersensitivity in mice to sheep red blood cells.small doses of outer membrane proteins (omp) of shigella flexneri injected intraperitoneally into mice 1 to 3 days before or 3 days after sensitization of animals with sheep erythrocytes were found to increase delayed hypersensitivity as measured by the footpad reaction. in contrast, administration of higher doses of omp resulted in suppression of hypersensitivity response. cell transfer experiments showed that the spleen cells from sensitized and omp treated mice transferred stimulating and sup ...19873326545
multiple drug-resistance in shigella flexneri isolated from a patient with human immunodeficiency virus.persistent shigellosis, due to shigella flexneri resistant to multiple antibiotics, developed in a 40-yr-old homosexual man with human immunodeficiency virus infection. the shigella strain demonstrated resistance to ampicillin, tetracycline, and trimethoprim-sulfamethoxazole. although shigella flexneri isolates resistant to trimethoprim-sulfamethoxazole are uncommon in the united states, laboratories should monitor resistance patterns through routine in vitro susceptibility testing.19873326711
phylogeny of lymphocyte heterogeneity: identification and separation of functionally distinct subpopulations of channel catfish lymphocytes with monoclonal antibodies.the purpose of this study was to identify monoclonal antibodies reactive with channel catfish t cells. since a variety of commercially available anti-human and mouse t cell reagents failed to react with channel catfish leucocytes, the development of anti-fish t cell monoclonal antibodies was undertaken. one of these reagents, designated mab 13c10, reacted with channel catfish surface immunoglobulin negative, but not positive, lymphocytes. this reagent, when used in "panning" protocols, was able ...19873326758
differential clinical features and stool findings in shigellosis and amoebic dysentery.to obtain information that could assist the clinician to differentiate between shigellosis and amoebic dysentery, we compared clinical features and stool findings in 58 adult male patients in bangladesh. mean values indicated that patients with invasive amoebiasis were older and had a longer prehospital illness, a lower body weight, less frequent fever, a lower haematocrit and a higher white blood cell count than patients with shigellosis. the mean number of faecal leucocytes per mm3 was signifi ...19873328339
[heterogeneity of shigella flexneri during selective synchronization].the study of a number of main growth characteristics of s. flexneri (s) cells during the first and second cycles of their cultivation in a solid culture medium in peshkov's chamber from seed material, nonsynchronized and synchronized by c. e. helmstetter's method, has revealed that this method does not permit obtaining completely synchronized cultures. four physiological cell clusters, differing in the time of generation, growth rate, size at the beginning and the end of the cycle and the time o ...19873328453
[campylobacter jejuni in patients with diarrhea].in this study the stools 125 patients with diarrhea applied to research hospital of medical school of atatürk university and the stools of 69 healthy persons were investigated for gastroenteritis agents c. jejuni as well as salmonella and shigella. of the zooparasiter agents protozoon and helminths were also investigated. we determined the mentioned agents in 28 of 125 patients (% 22.40). c. jejuni was isolated the most frequent cause of diarrhea with 8.80 percent. c. jejuni was isolated in high ...19873328817
[construction of a typhoid-dysentery bivalent strain with typhoid ty21a and dysentery t32-istrati strains]. 19873329424
[biochemical aspects of an endotoxin model for endogenous uveitis]. 19873329451
a controlled trial of nebulized aminoglycoside and oral flucloxacillin versus placebo in the outpatient management of children with cystic fibrosis.six children with cystic fibrosis who had persistently had pseudomonas aeruginosa isolated from their respiratory tract, completed a double-blind cross-over comparison of oral flucloxacillin and nebulized aminoglycoside versus double placebo. the patients had higher fev1 results at the end of the month of active treatment than after the month of placebo.19873329531
[trial use of embryonal and early-fetal thymus extracts in the treatment of non-healing skin defects. ii. crural ulcers in humans]. 19873329747
xylose lysine desoxycholate agar for recovering shigella dysenteriae 1 and shigella flexneri from faeces. 19873330107
on a survey of plasmid dna in the clinically isolated shigella strains in shanghai area and comparison with those from south east asia. 19873331655
a retrospective analysis of the occurrence of shigella flexneri serotypes in some african countries. 19873333153
outbreak of dysentery due to shigella flexneri type 2a. 19873333777
canine lymphoma: clinical considerations. 19863333887
canine lymphoma: clinical considerations. 19863333887
some biological properties of outer membrane proteins of shigella.outer membrane proteins (omp) derived from two antigenically different representatives of shigella (sh. flexneri 3a and sh. sonnei phase i) were tested for the toxicity, pyrogenicity, ability to induce shwartzman reaction as well as for their influence on the leukocyte system. ld50 dose determined on mice was 28 mg/kg for omp of sh. flexneri and 23 mg/kg for omp of sh. sonnei. both preparations injected intravenously to rabbits caused moderate increase of body temperature, expressed by the value ...19873334007
secretory activity and endocrine regulation of male accessory glands in the blood-sucking bug panstrongylus megistus (hemiptera: reduviidae).the epithelial cells of panstrongylus megistus male accessory glands (mag) present ultrastructural characteristics of a secretory cell. their secretory products are accumulated in the lumen of the four mag lobes. during the first 8 days of adult life a strong secretion activity occurs, accumulating enough material to produce the first spermatophore. cerebral neurosecretions as well as juvenile hormone are both involved in mag secretory activity regulation. juvenile hormone seems to be the respon ...19873334404
activities of some enzymes of phospholipid metabolism in cultured rat ventricular myocytes in normoxic and hypoxic conditions.phospholipid catabolism is thought to be one of the critical events in membrane injury during heart ischemia. in this work, the enzymes involved in phospholipid metabolism were studied in purified cultured ventricular myocytes in normoxic and hypoxic conditions. purified ventricular myocytes exhibited an alkaline phospholipase a activity which had sn-2 specificity and which was calcium dependent, and an acid phospholipase a activity with sn-1 specificity. these cells also exhibited lysophospholi ...19883334866
metaplastic breast carcinoma with epithelial phenotype of pseudosarcomatous components.a case of metaplastic breast carcinoma was subjected to an immunohistochemical study to characterize its apparent fibrosarcomatous and chondrosarcomatous elements. polyclonal and monoclonal antibodies against epithelial cell components such as keratin proteins, epithelial membrane antigen, membrane and cytoplasmic antigens of human mammary carcinoma cells, and carcinoembryonic antigen were used, as well as antibodies against the mesenchymal antigens, desmin, and vimentin. the cells with a mesenc ...19883337621
regulation of central cholecystokinin recognition sites by guanyl nucleotides.guanyl nucleotides affected the binding of radiolabeled cholecystokinin (cck) octapeptide to rodent cortical binding sites. micromolar quantities of a stable gtp analogue, guanylyl-imidodiphosphate (gppnp), resulted in a plateau where binding was decreased by 30%. in the presence of 25 microm gppnp, binding analysis revealed a decrease in affinity (increase in kd), without an apparent effect on the maximal number of binding sites. ki values for cck-related peptides shifted up to 1.6-fold. the ra ...19883339367
anterior perineal dislocation of the hip with fracture of the femoral head. a case report.traumatic anterior perineal hip dislocation with an associated fracture of the femoral head is a rare entity. a 26-year-old man injured in a motorcycle accident was treated by closed reduction of the dislocation within three hours after admission. however, several reports of patients with anterior hip dislocation with associated femoral head fractures were treated nonoperatively and had unfavorable results when treatment failed to achieve anatomical position of the fragments. consequently, this ...19883342566
lactic acidosis and recovery of neuronal function following cerebral hypoxia in vitro.the rat hippocampal slice preparation was used to study the combined effects of hypoxia and lactic acidosis on neuronal function. control slices were exposed to a standard hypoxic insult while being perfused with normal artificial cerebrospinal fluid (acsf). experimental slices were perfused with acsf containing 1.0, 2.0, 10.0 or 20.0 mm lactic acid, 30 min before and during the same standard hypoxic insult. following at 30-min recovery period the ability of these slices to respond to orthodromi ...19883345436
chemosensory anterior dorsal fin in rocklings (gaidropsarus and ciliata, teleostei, gadidae): somatotopic representation of the ramus recurrens facialis as revealed by transganglionic transport of hrp.the anterior dorsal fin in rocklings consists of a fringe of 50-80 delicate, vibratile rays, which are densely beset with epidermal chemosensory cells. the innervation of these cells is from the dorsal branch of the recurrent facial nerve, which also innervates all other fins and the skin of the trunk. this nerve carries at least three classes of fibres: small (0.5-1.5 micron in diameter), medium (1.5-4 micron), and large (greater than 4 micron). approximately 12,000 small and weakly myelinated ...19883346379
differences in synaptic inputs to preganglionic neurons in the dorsal and lateral band subdivisions of the cat sacral parasympathetic nucleus.in the cat, preganglionic neurons (pgns) found in the dorsal portion of the sacral parasympathetic nucleus (dorsal band or db cells) participate in bowel control, while those found along the lateral edge (lateral band or lb cells) influence bladder function. in order to determine whether differences in the synaptic inputs exist between these two populations, hrp was applied to the sacral ventral rootlets of cats, and the s2 cord segment was prepared for sequential light and electron microscopy. ...19883346386
corticotropin-induced changes in enzymatic activities of the post-pregnenolone steroidogenic pathway in rabbit adrenocortical cells.in an attempt to delineate the effect of corticotropin (acth) on post-pregnenolone steroidogenesis, the activity of enzymatic systems operative in conversion of pregnenolone into glucocorticoids and androgens was studied in adrenocortical cells from control rabbits and from animals treated with acth for 12 days (acth 1-24, 200 micrograms s.c. daily). the cells from acth-treated rabbits exhibited an increased overall steroidogenic capacity and produced much more cortisol (p less than 0.0005) as w ...19883347065
[meningoencephalitis and mononucleosis syndrome associated with acute infection by human immunodeficiency virus]. 19883347109
functionally illiterate worker also has 'right to understand'. 19883347451
the duck hepatitis b virus dna polymerase is tightly associated with the viral core structure and unable to switch to an exogenous template.the duck hepatitis b virus (dhbv) has a dna polymerase associated with it which uses the incomplete viral genome as endogenous template. a prerequisite for studying this polymerase is the availability of conditions to open viral cores without destroying their enzymatic activity. in this study, this was achieved by a brief treatment with low ph. dhbv dna in low-ph-treated cores was susceptible to digestion with deoxyribonuclease i and restriction enzymes, and large restriction fragments diffused ...19883347995
susceptibilities of hong kong isolates of multiply resistant shigella spp. to 25 antimicrobial agents, including ampicillin plus sulbactam and new 4-quinolones.seventy-two percent of 129 shigella isolates from two hong kong hospitals were shigella flexneri. twenty-six percent were s. sonnei, and there was only one isolate each of s. dysenteriae and s. boydii. ninety-six percent of the isolates were resistant to two or more antibiotics, and up to 11 resistances were seen in a single isolate. fifty-seven percent or more of these isolates were resistant to ampicillin, streptomycin, tetracycline, chloramphenicol, and sulfamethoxazole; and up to twenty-thre ...19883348608
[amino acid composition of the lymph and blood in fever reactions of different durations].the amino acid composition of the thoracic duct lymph and blood following fever reactions (fr) of various duration has been studied in the experiments on rabbits. the experiments have shown that some changes in protein metabolism in fr are primarily manifested in amino acid lymph composition. irrespective of fr duration the amino acid content in the lymph almost always increases, which confirms the opinion concerning catabolic process predominance in protein metabolism during fr. the lack of "cu ...19883349141
anti-inflammatory activity of oleoresin from brazilian copaifera.the oleoresin from brazilian copaifera species yielded copalic acid and sesquiterpenes and showed marked anti-inflammatory activity using various experimental models in rats. the oleoresin significantly inhibited carrageenin-induced pedal edema following oral doses from 0.70 to 2.69 ml/kg, but was somewhat less effective than 50 mg/kg calcium phenylbutazone. repeated administration of the oleoresin at a dose of 1.26 ml/kg for a 6-day period reduced granuloma formation with a response comparable ...19883352280
[tobacco or health: choose health]. 19883352398
chorioamnionitis: a study of organisms isolated in perinatal autopsies.the purpose of this study was to determine the validity of postmortem cultures in perinatal autopsies; in particular, those cases where there is gross or histologic evidence of chorioamnionitis. lung, liver, blood, and placental cultures were obtained from 159 neonatal autopsies with histologic evidence of chorioamnionitis at magee-women's hospital between january 1980 and july 1985. the criterion for chorioamnionitis was a polymorphonuclear leukocytic infiltrate in the placental membranes. the ...19883355095
septal thickness in human lungs assessed by computerized interactive morphometry.we have studied the feasibility of using interactive computerized morphometric methods for the quantitation of septal thickness in the lung with the goal of introducing objective criteria for the diagnosis and grading of pulmonary fibrosis and edema. after reviewing available methods, we have decided in favor of linear intercept measurements of septal profiles which are limited to the tissue and therefore not affected by the level of lung inflation. our method consisted in generating a graphic o ...19883357335
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