| e240v substitution increases catalytic efficiency toward ceftazidime in a new natural tem-type extended-spectrum beta-lactamase, tem-149, from enterobacter aerogenes and serratia marcescens clinical isolates. | the aim of this study was to characterize a novel extended-spectrum beta-lactamase that belongs to the tem family, the tem-149 enzyme, and that was isolated from the urine of two hospitalized patients from different hospitals in southern italy. the peculiarity of this enzyme was the finding of a valine residue at position 240. the array of amino acid substitutions found in tem-149 was as follows: e104k, r164s, m182t, and e240v. a reversion of a threonine residue at position 182 was also performe ... | 2008 | 18160520 |
| type 3 fimbriae, encoded by the conjugative plasmid pola52, enhance biofilm formation and transfer frequencies in enterobacteriaceae strains. | the conjugative plasmid pola52, which confers resistance to olaquindox and other antimicrobial agents through a multidrug efflux pump, was investigated for its ability to promote biofilm formation in escherichia coli. screening of a transposon-mutagenized pola52 clone library revealed several biofilm-deficient mutants, which all mapped within a putative operon with high homology to the mrkabcdf operon of klebsiella pneumoniae, where these genes are responsible for type 3 fimbriae expression, att ... | 2008 | 18174137 |
| succession of dominant and antagonistic lactic acid bacteria in fermented cucumber: insights from a pcr-based approach. | the goal of the investigation was to study the succession of major groups of lactic acid bacteria (lab) and their antagonism in salt-fermented cucumber using pcr. in a direct detection method as well as a short enrichment process, pcr enabled detection of leuconostoc and lactobacillus during early hours of fermentation. subsequently, lactobacillus and pediococcus emerged as the dominant genera. nucleic acid sequence of culture-independent clones confirmed the detection of pediococcus as a domina ... | 2008 | 18206770 |
| [antimicrobial effect of 4-(adamantyl-1)-1-(1-aminobutyl)benzol]. | antimicrobial activity in vitro of adamantine derivative in respect of aerobic and anaerobic microorganisms has been studied. in these conditions gram-positive bacteria and some representatives of gram-negative microorganisms (shigella flexneri, s. newvcastle, enterobacter aerogenes etc.) had maximal sensitivity (mic 0.6-5.0 and 0.1-5.0 mg/ml, respectively). pseudomonas aeruginosa were resistant to this substance (mic >1 mg/ml). | 2007 | 18217353 |
| decontamination effects of low-temperature plasma generated by corona discharge. part ii: new insights. | the second part of our paper presents the results of experiments with the decontamination of surfaces by low-temperature plasma generated by corona discharge in air at atmospheric pressure. a simple device is described and the effects of the corona discharge on model microorganisms, viz. the yeast candida albicans, gram-negative bacteria escherichia coli, enterobacter aerogenes, neisseria sicca, stenotrophomonas maltophilia, gram-positive bacteria deinococcus radiodurans, enterococcus faecium, s ... | 2007 | 18225640 |
| single daily high-dose tigecycline therapy of a multidrug-resistant (mdr) klebsiella pneumoniae and enterobacter aerogenes nosocomial urinary tract infection. | | 2007 | 18230562 |
| successive emergence of extended-spectrum beta-lactamase-producing and carbapenemase-producing enterobacter aerogenes isolates in a university hospital. | sixty-two clinical isolates of enterobacter aerogenes resistant to expanded-spectrum cephalosporins were collected between july 2003 and may 2005. among these isolates, 23 (37.1%) were imipenem (ipm) susceptible, and 39 (62.9%) were ipm insusceptible, of which 89.7% (35/39) were resistant and 10.3% (4/39) were intermediate. isolate genotypes were compared by pulsed-field gel electrophoresis. of 62 isolates, 48 belonged to epidemic pulsotype a (77.4%). this pulsotype included 37.5% and 58.4% of b ... | 2008 | 18234876 |
| molecular and descriptive epidemiologyof multidrug-resistant enterobacteriaceae in hospitalized infants. | to investigate the epidemiology of multidrug-resistant enterobacteriaceae (mdre) in hospitalized infants. | 2008 | 18251685 |
| clinical experience of serious infections caused by enterobacteriaceae producing vim-1 metallo-beta-lactamase in a greek university hospital. | the dissemination of acquired metallo-beta-lactamases (mbls) in members of the family enterobacteriaceae is regarded as an emerging clinical threat. the clinical characteristics and outcomes of 17 cases of infection due to mbl-producing isolates were analyzed. | 2008 | 18269335 |
| viper metalloproteinase (agkistrodon halys pallas) with antimicrobial activity against multi-drug resistant human pathogens. | metalloproteinases are abundant enzymes in crotalidae and viperidae snake venoms. snake venom metalloproteinases (svmps) comprise a family of zinc-dependent enzymes, which display many different biological activities. a 23.1 kda protein was isolated from agkistrodon halys (pallas, chinese viper) snake venom. the toxin is a single chain polypeptide with a molecular weight of 23146.61 and an n-terminal sequence (miqvllvticlavfpyqgssiiles) relatively similar to that of other metalloprotein-like pro ... | 2008 | 18297685 |
| occurrence of esbl & amp-c beta-lactamases & susceptibility to newer antimicrobial agents in complicated uti. | production of extended spectrum beta-lactamases (esbls) and ampc beta-lactamases are the most common mechanisms of antimicrobial resistance in gram negative bacilli. a prospective study was undertaken to know the occurrence of esbl and ampc producing strains and their antibiotic susceptibilities to newer agents to guide empirical therapy for complicated urinary tract infections. | 2008 | 18316858 |
| hypoxia-independent activation of hif-1 by enterobacteriaceae and their siderophores. | hypoxia inducible factor-1 (hif-1) is the key transcriptional regulator during adaptation to hypoxia. recent studies provide evidence for hif-1 activation during bacterial infections. however, molecular details of how bacteria activate hif-1 remain unclear. here, we pursued the role of bacterial siderophores in hif-1 activation during infection with enterobacteriaceae. | 2008 | 18325389 |
| [bacteriological study on adult chronic sinusitis operated after endoscopic sinus surgery]. | to explore the bacteria isolated from middle nasal meatus, maxillary sinus, ethmoid sinus and postoperative cavity of patients with chronic rhinosinusitis and their characteristics of antibiotic resistance. | 2007 | 18335747 |
| plasmid mediated antibiotic resistance in marine bacteria. | this research work was conducted in uppanar estuary to ascertain the role of plasmids in the antibiotic resistance of bacteria. water and sediment samples were collected for a period of three months. when tested against 20 antibiotics 22 mar strains were isolated from the samples, which were found resistant to 5-13 antibiotics. they belong to 7 genera and 10 species. gram-negative bacteria namely neisseria mucosa, n. sicca, branhamella catarrhalis, klebsiella ozaenae, citrobacterintermedius, pse ... | 2007 | 18380085 |
| antibacterial activity of some medicinal plant extracts. | antibacterial activity of hot aqueous and methanolic extracts prepared from six plants (terminallia chebula, terminallia bellerica, phyllanthus emblica, punica granatum, lawsonia alba and mikania micrantha) used in traditional folk medicines of india were screened against five pathogenic bacteria (staphylococcus aureus mtcc 2940, bacillus subtilis mtcc 441, escherichia coli mtcc 739, proteus vulgaris mtcc 426 and enterobacter aerogenes mtcc 111). the highest antibacterial potentiality was exhibi ... | 2008 | 18404337 |
| a versatile plasmid biosensor useful to identify quorum sensing luxr-family orphans in bacterial strains. | eight luxi-family gene promoters (luxi, cvii, ahli, rhli, cepi, phzi, trai and ppui) were cloned in tandem, upstream a promoterless lacz gene in a promoter probe vector yielding pmultiahlprom. this unique construct is useful in determining whether a bacterial strain not producing n-acyl homoserine lactone signal molecules (ahls) possesses orphan luxr type proteins able to respond to ahls and activate transcription from quorum sensing target genes. using pmultiahlprom, it was demonstrated that en ... | 2008 | 18420295 |
| construction of two lux-tagged hg2+-specific biosensors and their luminescence performance. | two hg2+-specific biosensors were constructed using bacterial luciferase as reporter gene and plasmid-free pseudomonas putida x4 and enterobacter aerogenes ntg-01 as host strains. the performance of x4 biosensor was compared with that of ntg-01 biosensor in the same assay conditions. the maximum bioluminescence for x4 (pmerrluxcdabe-kan) biosensor was found during the midexponential phase and that for ntg-01 (pmerrluxcdabe-kan) was at the late exponential phase. the shortest induction time of tw ... | 2008 | 18437376 |
| effect of linear alkylbenzene sulfonates on the growth of aerobic heterotrophic cultivable bacteria isolated from an agricultural soil. | an enrichment culture technique was used to isolate soil bacteria capable of growing in the presence of two different concentrations of linear alkylbenzene sulfonates (las) (10 and 500 microg ml(-1)). nine bacterial strains, representatives of the major colony types of aerobic heterotrophic cultivable bacteria in the enriched samples, were isolated and subsequently identified by pcr-amplification and partial sequencing of the 16s rrna gene. amongst the isolates, strains las05 (pseudomonas syring ... | 2008 | 18443905 |
| antimicrobial activity and synergism of some substituted flavonoids. | natural and synthetic substituted chalcones, flavones and flavanones were tested for antibacterial activity. in order to determine synergism, new combinations of substituted flavonoids against staphylococcus aureus, escherichia coli and enterobacter aerogenes were assayed. the results allow us to establish relationships between antimicrobial effect of the compounds and membrane structures of these microorganisms. when flavonoid combinations were employed a stronger effect was found against e. co ... | 2008 | 18481214 |
| fluoroquinolone resistance during 2000-2005: an observational study. | moxifloxacin is a respiratory fluoroquinolone with a community acquired pneumonia indication. unlike other fluoroquinolones used in our healthcare system, moxifloxacin's urinary excretion is low and thus we hypothesized that increased use of moxifloxacin is associated with an increase in fluoroquinolone resistance amongst gram negative uropathogens. | 2008 | 18501015 |
| cadmium(ii) complexes of the glycerophosphodiester-degrading enzyme gpdq and a biomimetic n,o ligand. | the glycerophosphodiester-degrading enzyme gpdq from enterobacter aerogenes is a promising bioremediator owing to its ability to degrade some organophosphate pesticides and diester products originating from the hydrolysis of nerve agents such as vx. here, the cadmium derivative of gpdq was prepared by reconstituting the apoenzyme. catalytic measurements with (cd(2+))(2)-gpdq and the phosphodiester substrate bis(4-nitrophenyl)phosphate yield k(cat) = 15 s(-1). the pk(a) of 9.4, determined from th ... | 2008 | 18535849 |
| evaluation of reuterin production in urogenital probiotic lactobacillus reuteri rc-14. | classified as a distinct species in 1980, lactobacillus reuteri strains have been used in probiotic formulations for intestinal and urogenital applications. in the former, the primary mechanism of action of l. reuteri sd2112 (atcc 55730) has been purported to be its ability to produce the antibiotic 3-hydroxypropionaldehyde (3-hpa), also known as reuterin. in the vagina, it has been postulated that probiotic lactobacillus reuteri rc-14 does not require reuterin production but mediates a restorat ... | 2008 | 18539802 |
| influence of extended-spectrum beta-lactams on gram-negative bacterial resistance. | the influence of extended- spectrum beta-lactams on gram-negative bacterial resistance was studied. | 2008 | 18541686 |
| reduction of acetoin to 2,3-butanediol in klebsiella pneumoniae: a new model. | fermentation of xylose by klebsiella pneumoniae (atcc 8724, formerly known as aerobacter aerogenes) carried out in our laboratory yields 2,3-butanediol as the major product. experimental data obtained in this work cannot be explained by the model presently in the literature for the formation of 2,3-butanediol isomers from acetoin isomers. a new model is proposed with the existence of two acetoin reductases and an acetoin racemase. the two reductases were separated and their stereospecificity det ... | 1983 | 18548546 |
| butanediol production by aerobacter aerogenes nrrl b199: effects of initial substrate concentration and aeration agitation. | butanediol production by aerobacter aerogenes nrrl b199 grown on glucose requires an optimal rate of aeration for the obtention of butanediol 2, 3. in the absence of air, aerobacter aerogenes nrrl b199 growth and production are weak. agitation-aeration is necessary for producing the biomass, but an excess of oxygen proves to be toxic with regard to metabolite production. oxygen is a limiting substrate with regard to growth and an inhibitor with regard to the specific metabolite productivity. thi ... | 1984 | 18551701 |
| enzymatic production of l-serine with a feedback control system for formaldehyde addition. | serine hydroxymethyltransferase (shmt) in the form of crude extract from a recombinant strain of klebsiella aerogenes was used for the production of l-serine from glycine and formaldehyde (hcho). a stirred tank bio-reactor with a continuous feed of hcho (37%) was employed. since the performance of the serine bioreactor was heavily dependent on how hcho was fed, an automatic feedback control system was developed for hcho delivery utilizing the phenomenon of formol titration. this control procedur ... | 1986 | 18553868 |
| enzymatic production of l-serine. | serine hydroxymethyltransferase (shmt) in the form of crude extract form a recombinant strain of klebsiella aerogenes was used to study the production of l-serine from glycine and formaldehyde (hcho). shmt activity linearly increased with temperature (30-50 degrees c). addition of exogenous cofactors, tetrahydrofolic acid and pyridoxal-phosphate, significantly increased shmt activity. the ph optimum of the shmt catalyzed l-serine synthesis step was between 8.0 and 8.5. the k(m) for glycine was 1 ... | 1986 | 18555404 |
| in vivo development of carbapenem resistance in clinical isolates of enterobacter aerogenes producing multiple beta-lactamases. | four clinical strains of extended-spectrum beta-lactamase- and ampc-producing enterobacter aerogenes were isolated successively from a liver transplantation patient. isolates c(1) and c(2) were isolated prior to carbapenem therapy, whilst isolates c(3) and c(4) were recovered after 40 days of carbapenem therapy. the homology of these strains was analysed by pulsed-field gel electrophoresis (pfge). beta-lactamases were analysed by isoelectric focusing, polymerase chain reaction (pcr) and sequenci ... | 2008 | 18556176 |
| maintenance metabolism and carbon fluxes in bacillus species. | abstract: | 2008 | 18564406 |
| the culture site of the gallbladder affects recovery of bacteria in symptomatic cholelithiasis. | traditional methods for bile culturing may miss a large number of underlying bacterial infections that could lead to acute or chronic cholecystitis. | 2008 | 18568139 |
| antibacterial and preliminary phytochemical and physico-chemical analysis of eucalyptus citriodora hk leaf. | the present communication deals with some studies on the antibacterial, physico-chemical and phytochemical parameters of different extracts of eucalyptus citriodora leaf. the antibacterial study was performed using the agar ditch method on some clinically important bacteria, namely pseudomonas pseudoalcaligenes, proteus vulgaris, citrobacter freundii, staphylococcus subflava, bacillus megaterium, and enterobacter aerogenes. physico-chemical parameters namely water, methanol, 1,4-dioxane, dmf, ac ... | 2008 | 18569717 |
| single-cell protein from methanol with enterobacter aerogenes. | an identifiedenterobacter aerogenesutilizing methanol as a sole carbon source was studied for the optimization of biomass production and the reduction of its nucleic acid content. results indicated that the highest yield and conversion were obtained at 0.5% methanol. the addition of seawater as a source of trace elements has an adverse effect. however, the addition of urea as source of nitrogen enhanced the growth of e. aerogenes. heat shock at 60 degrees c for 1 min followed by incubation at 50 ... | 1987 | 18576425 |
| combined physico-chemical treatments based on enterocin as-48 for inactivation of gram-negative bacteria in soybean sprouts. | enterocin as-48 was tested for decontamination of soybean sprouts against gram-negative bacteria. although treatment with bacteriocin alone had no effect on salmonella enterica, a synergistic antimicrobial effect was detected at ph 9.0 and in combination with moderate heat treatment. greatest inactivation was achieved for sprouts heated for 5 min at 65 degrees c in an alkaline (ph 9.0) enterocin as-48 solution of 25 microg/ml. bactericidal activity against s. enterica increased greatly when ente ... | 2008 | 18577412 |
| efficacy of contact lens systems against recent clinical and tap water acanthamoeba isolates. | a recent increase in acanthamoeba keratitis (ak) cases has been associated with complete moistureplus, although many cases used other solutions. complete moistureplus contains taurine and hydroxypropyl methylcellulose, unlike other multipurpose solutions (mpss). the purpose of this study is to (1) determine contact lens solution efficacy against recent clinical and tap water acanthamoeba isolates and (2) determine whether taurine inclusion increases acanthamoeba survival against contact lens sol ... | 2008 | 18580265 |
| dictyostelium transcriptional responses to pseudomonas aeruginosa: common and specific effects from pao1 and pa14 strains. | pseudomonas aeruginosa is one of the most relevant human opportunistic bacterial pathogens. two strains (pao1 and pa14) have been mainly used as models for studying virulence of p. aeruginosa. the strain pa14 is more virulent than pao1 in a wide range of hosts including insects, nematodes and plants. whereas some of the differences might be attributable to concerted action of determinants encoded in pathogenicity islands present in the genome of pa14, a global analysis of the differential host r ... | 2008 | 18590548 |
| mechanism of enhanced oxygen transfer in fermentation using emulsified oxygen-vectors. | limitations of oxygen transfer in fermentation can be solved using auxiliary liquids immiscible in the aqueous phase. the liquids (called oxygen-vectors) used in this study were hydrocarbon (n-dodecane) and perfluorocarbon (forane f66e) in which oxygen is highly soluble (54.9 mg/l in n-dodecane and 118 mg/l in forane f66e at 35 degrees c in contact with air at atmospheric pressure). it has been demonstrated that the use of n-dodecane emulsion in a culture of aerobacter aerogenes enabled a 3. 5-f ... | 1990 | 18592535 |
| induction of nucleoside phosphorylase in enterobacter aerogenes and enzymatic synthesis of adenine arabinoside. | nucleoside phosphorylases (npases) were found to be induced in enterobacter aerogenes dgo-04, and cytidine and cytidine 5'-monophosphate (cmp) were the best inducers. five mmol/l to fifteen mmol/l cytidine or cmp could distinctly increase the activities of purine nucleoside phosphorylase (pnpase), uridine phosphorylase (upase) and thymidine phosphorylase (tpase) when they were added into medium from 0 to 8 h. in the process of enzymatic synthesis of adenine arabinoside from adenine and uracil ar ... | 2008 | 18600781 |
| utilization of the tricarboxylic acid cycle, a reactor design criterion for the microaerobic production of 2,3-butanediol. | a new parameter, the relative utilization of tricarboxylic acid (tca) cycle beta, is introduced to quantitatively account for the involvement of fermentation pathways and tca cycle in the utilization of oxygen under oxygen-limiting (microaerobic) conditions. with the facultative anaerobe enterobacter aerogenes, which produces 2,3-butanediol, a method is proposed to calculate beta from measurement of metabolites and exhaust gas. in continuous culture beta was found to be small under oxygen limita ... | 1992 | 18601216 |
| bacteremia due to enterobacter spp. in cancer patients--analysis of 51 episodes. | fifty one episodes of bacteremia due to enterobacter spp. appearing within 7 years among 12 301 admissions in a single cancer institution were studied for risk factors, clinical presentation and outcome. fifteen episodes were due to enterobacter aerogenes, 23 due to e. cloacae and 13 due to e. agglomerans. the proportion of bacteremia due to enterobacter spp. among gram-negative bacteremias was 10.1% and infection associated mortality was 13.8%. the incidence in 1989-1995 varied from 3.7 to 8.7% ... | 1997 | 18611814 |
| synthesis and in-vitro antibacterial activity of 5-substituted 1-methyl-4-nitro-1h-imidazoles. | a series of 5-substituted 1-methyl-4-nitro-1h-imidazole derivatives were synthesized and evaluated for in-vitro antibacterial activity against a panel of microorganisms including staphylococcus aureus, staphylococcus epidermidis, bacillus subtilis, escherichia coli, klebsiella pneumonia, enterobacter aerogenes, and helicobacter pylori using conventional agar dilution method. among the test compounds, 1-methyl-4-nitro-5-(phenylsulfonyl)-1h-imidazole was the most potent against gram-positive bacte ... | 2008 | 18618489 |
| bacteriocin-producing lactobacillus strains isolated from poto poto, a congolese fermented maize product, and genetic fingerprinting of their plantaricin operons. | thirty one bacteriocin-producing lactobacillus isolates were identified among 135 lactobacilli isolated from the congolese fermented maize product poto poto, during the preparation and from the finished product. using species-specific pcr and 16s rrna gene sequencing, 28 and 3 isolates were identified as l. plantarum and l. fermentum, respectively. cluster analysis of rapd-pcr fingerprints revealed two main groups (g1 and g2) plus the l. fermentum isolate c4-13. group g1 contained 23 isolates wi ... | 2008 | 18620772 |
| a tripartite efflux pump involved in gastrointestinal colonization by klebsiella pneumoniae confers a tolerance response to inorganic acid. | the colonization of the gastrointestinal tract of patients by the opportunistic gram-negative bacillus klebsiella pneumoniae generally occurs prior to the development of nosocomial infections. mutant strain c-81 was isolated owing to its reduced capacity to colonize the digestive tract in a murine model following transposon mutagenesis (n. maroncle, d. balestrino, c. rich, and c. forestier, infect. immun. 70:4729-4734, 2002). nucleotide sequence analysis showed that the transposon had inserted i ... | 2008 | 18644883 |
| malonate-bound structure of the glycerophosphodiesterase from enterobacter aerogenes (gpdq) and characterization of the native fe2+ metal-ion preference. | the structure of a malonate-bound form of the glycerophosphodiesterase from enterobacter aerogenes, gpdq, has been refined at a resolution of 2.2 a to a final r factor of 17.1%. the structure was originally solved to 2.9 a resolution using sad phases from zn2+ metal ions introduced into the active site of the apoenzyme [jackson et al. (2007), j. mol. biol. 367, 1047-1062]. however, the 2.9 a resolution was insufficient to discern significant details of the architecture of the binuclear metal cen ... | 2008 | 18678932 |
| nxl104 combinations versus enterobacteriaceae with ctx-m extended-spectrum beta-lactamases and carbapenemases. | the beta-lactamase landscape is changing radically, with ctx-m types now the most prevalent extended-spectrum beta-lactamases (esbls) worldwide, except maybe in the usa. in addition, there are growing numbers of enterobacteriaceae with kpc and metallo-carbapenemases. we examined whether combinations of oxyimino-cephalosporins with nxl104, a novel non-beta-lactam beta-lactamase inhibitor, overcame these resistances. | 2008 | 18689875 |
| kinetic flux profiling for quantitation of cellular metabolic fluxes. | this protocol enables quantitation of metabolic fluxes in cultured cells. measurements are based on the kinetics of cellular incorporation of stable isotope from nutrient into downstream metabolites. at multiple time points, after cells are rapidly switched from unlabeled to isotope-labeled nutrient, metabolism is quenched, metabolites are extracted and the extract is analyzed by chromatography-mass spectrometry. resulting plots of unlabeled compound versus time follow variants of exponential de ... | 2008 | 18714301 |
| ethanolamine activates a sensor histidine kinase regulating its utilization in enterococcus faecalis. | enterococcus faecalis is a gram-positive commensal bacterium of the human intestinal tract. its opportunistic pathogenicity has been enhanced by the acquisition of multiple antibiotic resistances, making the treatment of enterococcal infections an increasingly difficult problem. the extraordinary capacity of this organism to colonize and survive in a wide variety of ecological niches is attributable, at least in part, to signal transduction pathways mediated by two-component systems (tcs). here, ... | 2008 | 18776017 |
| membrane permeability and regulation of drug "influx and efflux" in enterobacterial pathogens. | in enterobacteriaceae, membrane permeability is a key in the level of susceptibility to antibiotics. modification of the bacterial envelope by decreasing the porin production or increasing the expression of efflux pump systems has been reported. these phenomena are frequently associated with other resistance mechanisms such as alteration of antibiotics or modification of the drug targets, in various clinical isolates showing a multi drug resistant phenotype (mdr). in escherichia coli, enterobact ... | 2008 | 18781921 |
| identification and evolution of drug efflux pump in clinical enterobacter aerogenes strains isolated in 1995 and 2003. | the high mortality impact of infectious diseases will increase due to accelerated evolution of antibiotic resistance in important human pathogens. development of antibiotic resistance is a evolutionary process inducing the erosion of the effectiveness of our arsenal of antibiotics. resistance is not necessarily limited to a single class of antibacterial agents but may affect many unrelated compounds; this is termed 'multidrug resistance' (mdr). the major mechanism of mdr is the active expulsion ... | 2008 | 18787654 |
| wound healing, anti-microbial and antioxidant potential of dendrophthoe falcata (l.f) ettingsh. | dendrophthoe falcata (l.f) ettingsh (loranthaceae) of the order santalales, is used ethnomedicinally for treating ulcers, asthma, impotence, paralysis, skin diseases, and wounds. in this context, validations of the ethnotherapeutic claims of the plant in wound healing activity was studied, besides anti-microbial activity and antioxidant activity were performed to understand the mechanism of wound healing potency. the ethanolic extract of aerial parts of dendrophthoe falcata ethanolic extract (df ... | 2008 | 18790035 |
| dualistic acidic and neutral glucose fermentation balance in small intestine: simulation in vitro. | intestinal microbes live in diminished or deprived oxygen conditions. facultative anaerobic bacteria of the family enterobacteriaceae ferment glucose in the gut using two main pathways: mixed acid and neutral fermentations. the aim of the present study was to clarify the roles of these fermentations in an in vitro model. acid-producing escherichia coli atcc 25922 and neutral end-products producing klebsiella mobilis atcc 13048 were cultured in a portable microbe enrichment unit (pmeu) which perm ... | 2008 | 18804970 |
| efficacy of plant essential oils against foodborne pathogens and spoilage bacteria associated with ready-to-eat vegetables: antimicrobial and sensory screening. | the objectives of this study were to evaluate the antimicrobial activity of plant essential oils (eos) against foodborne pathogens and key spoilage bacteria pertinent to ready-to-eat vegetables and to screen the selected eos for sensory acceptability. the eos basil, caraway, fennel, lemon balm, marjoram, nutmeg, oregano, parsley, rosemary, sage, and thyme were evaluated. the bacteria evaluated were listeria spp., staphylococcus aureus, lactobacillus spp., bacillus cereus, salmonella, enterobacte ... | 2008 | 18810868 |
| digestion of rice straw and oil palm fronds by microflora from rumen and termite bacteria, in vitro. | the digestion and volatile fatty acid (vfa) production from rice straw and oil palm fronds by cellulolytic bacteria isolated from the termite coptotermes curvignathus were investigated. the bacteria were acinetobacter strain raminalimon, enterobacter aerogenes strain razmin c, enterobacter cloacae strain razmin b, bacillus cereus strain razmin a and chryseobacterium kwangyangense strain cb. acinetobacter strain raminalimon is an aerobic bacterium, while the other species are facultative anaerobe ... | 2008 | 18817130 |
| the structure of urease activation complexes examined by flexibility analysis, mutagenesis, and small-angle x-ray scattering. | conformational changes of klebsiella aerogenes urease apoprotein (ureabc)(3) induced upon binding of the ured and uref accessory proteins were examined by a combination of flexibility analysis, mutagenesis, and small-angle x-ray scattering (saxs). proflex analysis of urease provided evidence that the major domain of ureb can move in a hinge-like motion to account for prior chemical cross-linking results. rigidification of the ureb hinge region, accomplished through a g11p mutation, reduced the e ... | 2008 | 18823937 |
| substrate-promoted formation of a catalytically competent binuclear center and regulation of reactivity in a glycerophosphodiesterase from enterobacter aerogenes. | the glycerophosphodiesterase (gpdq) from enterobacter aerogenes is a promiscuous binuclear metallohydrolase that catalyzes the hydrolysis of mono-, di-, and triester substrates, including some organophosphate pesticides and products of the degradation of nerve agents. gpdq has attracted recent attention as a promising enzymatic bioremediator. here, we have investigated the catalytic mechanism of this versatile enzyme using a range of techniques. an improved crystal structure (1.9 a resolution) i ... | 2008 | 18831553 |
| risk of misidentification of enterobacter aerogenes inducible cephalosporinases. | | 2008 | 18835042 |
| experimental and qsar of acetophenones as antibacterial agents. | antibacterial activity of 20 acetophenone derivatives was evaluated against two gram-positive and three gram-negative organisms, namely, bacillus subtilis ncim 2718, staphylococcus aureus ncim5021, salmonella typhi ncim2501, enterobacter aerogenes ncim5139, and proteus vulgaris ncim2813 by two-fold dilution method. the most active amongst this group of compounds were 4-methyl, 2-hydroxy, 3-bromo, 4-ethoxy, 3-nitro and 4-nitro acetophenones. quantitative structure-activity relationships were deve ... | 2008 | 18844677 |
| [recent evolution and characterization of extended-spectrum beta-lactamase producing enterobacteria in the chu of nice (2005-2007)]. | esbl producing enterobacteria (e-esbl+) are always a public health concern, mainly due to increase of ctx-m beta-lactamase. so, 542 new strains were isolated in the chu de nice during the 2005-2007 period. the aim of this work was to characterize the esbl and to type isolates suspected to be implicated in outbreaks. | 2008 | 18848404 |
| [differential diagnosis of aerobacter aerogenes and escherichia coli by means of the feso4 reaction]. | | 1947 | 18918685 |
| bacterial contamination in drinking water: a case study in rural areas of northern rajasthan, india. | the objective of this study was to determine the bacterial contaminations in drinking water samples collected form some rural habitations of northern rajasthan, india. a total of ten bacterial species: escherichia coli, pseudomonas aeruginosa, enterobacter aerogenes, klebsiella sp, proteus vulgaris, alcaligenes faecalis, bacillus cereus, staphylococcus aureus, streptococcus lactis and micrococcus luteum were identified form drinking water samples. the bacteria belonging to the family enterobacte ... | 2009 | 19023670 |
| absorption, distribution, metabolism, and excretion of 2,2-bis(bromomethyl)-1,3-propanediol in male fischer-344 rats. | 2,2-bis(bromomethyl)-1,3-propanediol (bmp) is a brominated flame retardant, previously shown to be a multisite carcinogen in experimental animals. studies were performed to characterize the dispositional and metabolic fate of bmp after oral or intravenous administration to male fischer-344 rats. after a single oral administration of [(14)c]bmp (10 or 100 mg/kg) >80% of the low dose and 48% of the high dose were excreted by 12 h in the urine predominantly as a glucuronide metabolite. after repeat ... | 2009 | 19029203 |
| absorption, distribution, metabolism, and excretion of 2,2-bis(bromomethyl)-1,3-propanediol in male fischer-344 rats. | 2,2-bis(bromomethyl)-1,3-propanediol (bmp) is a brominated flame retardant, previously shown to be a multisite carcinogen in experimental animals. studies were performed to characterize the dispositional and metabolic fate of bmp after oral or intravenous administration to male fischer-344 rats. after a single oral administration of [(14)c]bmp (10 or 100 mg/kg) >80% of the low dose and 48% of the high dose were excreted by 12 h in the urine predominantly as a glucuronide metabolite. after repeat ... | 2009 | 19029203 |
| microbiological and toxicological effects of perla black bean (phaseolus vulgaris l.) extracts: in vitro and in vivo studies. | we investigated the microbiological and toxicological effects of three perla black bean extracts on the growth and culture of selected pathogenic microorganisms, the toxicity over vero cell lines and an in vivo rat model. three different solvents were used to obtain perla black bean extracts. all three perla black bean extracts were tested for antibacterial and antiparasitic activity and further analysed for intrinsic cytotoxicity (ic(50)). methanol perla black bean extract was used for acute to ... | 2009 | 19053992 |
| purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes. | the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ... | 2009 | 19060149 |
| purine utilization by klebsiella oxytoca m5al: genes for ring-oxidizing and -opening enzymes. | the enterobacterium klebsiella oxytoca uses a variety of inorganic and organic nitrogen sources, including purines, nitrogen-rich compounds that are widespread in the biosphere. we have identified a 23-gene cluster that encodes the enzymes for utilizing purines as the sole nitrogen source. growth and complementation tests with insertion mutants, combined with sequence comparisons, reveal functions for the products of these genes. here, we report our characterization of 12 genes, one encoding gua ... | 2009 | 19060149 |
| [detection of ampc beta-lactamases in gram-negative bacteria isolated from urine]. | ampc beta-lactamases, enzymes produced by a range of gram-negative bacteria and able to hydrolyse penicillins, monobactams, cephalosporins and cephamycins, are not inhibited by beta-lactamase inhibitors. they are spread via plasmids and pose a huge epidemiological and diagnostic problem. the production of ampc b-lactamases was tested in various gram-negative bacteria. of strains isolated from urine of patients diagnosed with urinary tract infection, we selected those that were resistant to third ... | 2008 | 19069027 |
| the action of barbitone on aerobacter aerogenes (klebsiella aerogenes). | | 1967 | 19108244 |
| update on antimicrobial susceptibility rates among gram-negative and gram-positive organisms in the united states: results from the tigecycline evaluation and surveillance trial (test) 2005 to 2007. | the tigecycline evaluation and surveillance trial (ttest) is a global surveillance study initiated in 2004.its goal is to assess the in vitro activity of the glycylcycline, tigecycline, and comparator antimicrobials. objective: the aim of this study was to measure the in vitro activity of a panel of antimicrobial agents against gram-negative and gram-positive organisms collected in the united states in 2005, 2006, and 2007. | 2008 | 19108792 |
| direct production of l-tagatose from l-psicose by enterobacter aerogenes 230s. | l-tagatose was produced directly from l-psicose by subjecting the same biomass suspension to microbial reduction followed by oxidation using a newly isolated bacteria enterobacter aerogenes 230s. after various optimizations, it was observed that cells grown on xylitol have the best conversion potential. moreover, e. aerogenes 230s converted l-psicose to l-tagatose at a faster rate in the presence of polyols such as glycerol, d-sorbitol, ribitol, l-arabitol, d-mannitol and xylitol. at 5% substrat ... | 2008 | 19111643 |
| antifungal and antibacterial activities of mexican tarragon (tagetes lucida). | mexican tarragon (tagetes lucida cv. asteraceae: campanulatae) is an important, nutritious plant and an effective herbal medicine. seven coumarins, 7,8-dihydroxycoumarin (4), umbelliferone (7-hydroxycoumarin) (5), scoparone (6,7-dimethoxycoumarin) (7), esculetin (6,7-dihydroxycoumarin) (11), 6-hydroxy-7-methoxycoumarin (12), herniarin (7-methoxycoumarin) (13), and scopoletin (6-methoxy-7-hydroxycoumarin) (14), and three flavonoids, patuletin (18), quercetin (19), and quercetagetin (20), were iso ... | 2006 | 19127719 |
| ampc beta-lactamases. | summary: ampc beta-lactamases are clinically important cephalosporinases encoded on the chromosomes of many of the enterobacteriaceae and a few other organisms, where they mediate resistance to cephalothin, cefazolin, cefoxitin, most penicillins, and beta-lactamase inhibitor-beta-lactam combinations. in many bacteria, ampc enzymes are inducible and can be expressed at high levels by mutation. overexpression confers resistance to broad-spectrum cephalosporins including cefotaxime, ceftazidime, an ... | 2009 | 19136439 |
| enzymatic transesterification of jatropha oil. | transesterification of jatropha oil was carried out in t-butanol solvent using immobilized lipase from enterobacter aerogenes. the presence of t-butanol significantly reduced the negative effects caused by both methanol and glycerol. the effects of various reaction parameters on transesterification of jatropha oil were studied. | 2009 | 19144158 |
| occurrence of efflux mechanism and cephalosporinase variant in a population of enterobacter aerogenes and klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases. | we investigated the occurrence of multidrug resistance in 44 enterobacter aerogenes and klebsiella pneumoniae clinical isolates. efflux was involved in resistance in e. aerogenes isolates more frequently than in k. pneumoniae isolates (100 versus 38% of isolates) and was associated with the expression of phenylalanine arginine beta-naphthylamide-susceptible active efflux. acra-tolc overproduction in e. aerogenes isolates was noted. an analysis of four e. aerogenes isolates for which cefepime mic ... | 2009 | 19164140 |
| blood culture confirmed bacterial sepsis in neonates in a north indian tertiary care center: changes over the last decade. | the spectrum of organisms causing sepsis is different in developing countries. data on the recent trends of organisms causing sepsis are limited. this study was conducted in a tertiary care neonatal unit in northern india. all inborn babies with blood-culture-positive sepsis from 1995 to 2006 were divided into two epochs, viz. 1995 to 1998 (epoch i) and 2001 to 2006 (epoch ii). organisms were grouped into early (<72 h) and late onset (> or =72 h) sepsis groups. the overall incidence of sepsis, t ... | 2009 | 19168958 |
| antimicrobial activity of some thiourea derivatives and their nickel and copper complexes. | five thiourea derivative ligands and their ni(2+) and cu(2+) complexes have been synthesized. the compounds were screened for their in vitro anti-bacterial activity using gram-positive bacteria (two different standard strains of staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, streptococcus pyogenes, bacillus cereus) and gram-negative bacteria (esherichia coli, pseudomonas aeruginosa, enterobacter cloacae, proteus vulgaris, enterobacter aerogenes) and in vitro anti-yeast ... | 2009 | 19169199 |
| isothiocyanate profile and selective antibacterial activity of root, stem, and leaf extracts derived from raphanus sativus l. | acetone and hexane extracts derived from the root, stem, and leaf of raphanus sativus were investigated for their antibacterial activity against foodborne and resistant pathogens, such as bacillus subtilis, staphylococcus aureus, staphylococcus epidermidis, enterococcus faecalis, salmonella typhimurium, enterobacter aerogenes, enterobacter cloacae, and escherichia coli. total and individual isothiocyanate (itc) components and their relationship with the antibacterial activity of r. sativus were ... | 2009 | 19182965 |
| identification of [14c]fluasterone metabolites in urine and feces collected from dogs after subcutaneous and oral administration of [14c]fluasterone. | the objective of this research was the identification of the metabolic profile of fluasterone, a synthetic derivative of dehydroepiandrosterone, in dogs treated orally or subcutaneously with [4-(14)c]fluasterone. separation and characterization techniques used to identify the principal metabolites of fluasterone in urine and feces included high-performance liquid chromatography (hplc), liquid scintillation spectrometry, hplc/tandem mass spectrometry, and nmr. in urine, the majority of the radioa ... | 2009 | 19196848 |
| accumulation of plasmid-mediated fluoroquinolone resistance genes, qepa and qnrs1, in enterobacter aerogenes co-producing rmtb and class a beta-lactamase lap-1. | a new plasmid-mediated fluoroquinolone efflux pump gene, qepa, is known to be associated with the rmtb gene, which confers high-level resistance to aminoglycosides. we investigated the qepa gene in 573 ampc-producing enterobacteriaceae including one citrobacter freundii known to harbor rmtb. of them, two clonally unrelated e. aerogenes harbored qepa. both isolates co-harbored rmtb, qnrs1, qepa, and bla(lap-1) on an incfi type plasmid. the qepa was flanked by two copies of is26 containing iscr3c, ... | 2009 | 19201742 |
| molecular mechanisms disrupting porin expression in ertapenem-resistant klebsiella and enterobacter spp. clinical isolates from the uk. | the aim of this study was to investigate relatedness and molecular mechanism(s) of ertapenem resistance in clinical isolates of klebsiella spp. (n = 28) and enterobacter spp. (n = 27) referred from multiple hospitals to the uk national reference laboratory. | 2009 | 19233898 |
| role of tolc in klebsiella oxytoca resistance to antibiotics. | the gram-negative human pathogen klebsiella oxytoca is often resistant to several antibiotics such as fluoroquinolones, erythromycin, tetracycline, chloramphenicol and others. the aim of this study was to look at the mechanisms leading to this resistance and particularly the role of tolc and efflux mechanisms in determining resistance. | 2009 | 19240073 |
| optimization of the enzymatic hydrolysis and analysis of plasma conjugated gamma-cehc and sulfated long-chain carboxychromanols, metabolites of vitamin e. | natural forms of vitamin e are metabolized by omega-hydroxylation and beta-oxidation of the hydrophobic side chain to generate urinary-excreted 2-(beta-carboxyethyl)-6-hydroxychroman (cehc) and cehc conjugates (sulfate, glucuronide, or glucoside). we recently showed that sulfated long-chain carboxychromanols, the conjugated intermediate beta-oxidation products, are formed from tocopherols and tocotrienols in human cells and in rats. cehc conjugates have been quantified after being converted to i ... | 2009 | 19250920 |
| geraniol restores antibiotic activities against multidrug-resistant isolates from gram-negative species. | the essential oil of helichrysum italicum significantly reduces the multidrug resistance of enterobacter aerogenes, escherichia coli, pseudomonas aeruginosa, and acinetobacter baumannii. combinations of the two most active fractions of the essential oil with each other or with phenylalanine arginine beta-naphthylamide yield synergistic activity. geraniol, a component of one fraction, significantly increased the efficacy of beta-lactams, quinolones, and chloramphenicol. | 2009 | 19258278 |
| synthesis, biological evaluation, mechanism of action and quantitative structure-activity relationship studies of chalcones as antibacterial agents. | forty-eight chalcone analogs were synthesized and their in vitro antibacterial activity against staphylococcus aureus ncim 5021, bacillus subtilis ncim 2718, phaseolus vulgaris ncim 2813, escherichia coli ncim 2931, salmonella typhi 2501 and enterobacter aerogenes ncim 5139 were evaluated by microdilution broth assay. quantitative structure-activity relationships were developed for all the cases (r(2) = 0.68-0.79; r(2)(adj) = 0.58-0.78; q(2) = 0.51-0.68; f = 13.02-61.51). size, polarizability, e ... | 2009 | 19291103 |
| novel ctx-m {beta}-lactamase genotype distribution and spread into multiple species of enterobacteriaceae in changsha, southern china. | the aim of this study was to undertake a survey of the occurrence of ctx-m and shv extended-spectrum beta-lactamase (esbl) genotypes in enterobacteriaceae from hunan province, china. | 2009 | 19297379 |
| effects of an alternative cefepime dosing strategy in pulmonary and bloodstream infections caused by enterobacter spp, citrobacter freundii, and pseudomonas aeruginosa: a single-center, open-label, prospective, observational study. | various dosing strategies for cefepime have been developed in an effort to maximize pharmacodynamic exposure of this agent against gram-negative infections. an assessment of cefepime dosing strategies is warranted given recent reports of poorer treatment outcomes associated with cefepime compared with other antibiotics, particularly in patients infected with gram-negative organisms with elevated mics. | 2009 | 19302902 |
| regulation of glycerol metabolism in enterobacter aerogenes nbrc12010 under electrochemical conditions. | enterobacter aerogenes nbrc12010 was able to ferment glycerol to ethanol and hydrogen gas. fermentation of glycerol ceased in the stationary phase of growth, and it was activated by electrochemical reactions using thionine as an electron transfer mediator from bacterial cells to an electrode. using resting cells of e. aerogenes nbrc12010 in only citrate buffer solution, the cells did not consume glycerol at all, but they could metabolize glucose. these results suggest that the regulation of glyc ... | 2009 | 19352646 |
| bacterial indicators of pollution of the douala lagoon, cameroon: public health implications. | indiscriminate disposal of untreated wastes which are often heavily laden with sewage microorganisms some of which are pathogenic to humans into aquatic environments near cities could serve as potential dangers to human health. | 2008 | 19357756 |
| strategies for acquiring the phospholipid metabolite inositol in pathogenic bacteria, fungi and protozoa: making it and taking it. | myo-inositol (inositol) is an essential nutrient that is used for building phosphatidylinositol and its derivatives in eukaryotes and even in some eubacteria such as the mycobacteria. as a consequence, fungal, protozoan and mycobacterial pathogens must be able to acquire inositol in order to proliferate and cause infection in their hosts. there are two primary mechanisms for acquiring inositol. one is to synthesize inositol from glucose 6-phosphate using two sequentially acting enzymes: inositol ... | 2009 | 19383710 |
| detection of plasmid-mediated quinolone resistance genes in clinical isolates of enterobacter spp. in spain. | we have studied by pcr and dna sequencing the presence of the qnra, qnrb, qnrs, aac(6')-ib-cr, qepa, inti1, and iscr1 genes in 200 clinical isolates of enterobacter cloacae (n = 153) and e. aerogenes (n = 47) consecutively collected between january 2004 and october 2005 in two hospitals located in santander (northern spain) and seville (southern spain). mutations in the quinolone resistance-determining region of gyra and parc also were investigated in organisms containing plasmid-mediated quinol ... | 2009 | 19386836 |
| biological activity of alkaloids from solanum dulcamara l. | alkaloids are well known for their antimicrobial activity. though all natural alkaloids come from plants, not all plants produce alkaloids. plants of the solanaceae family are known for their high alkaloid content. alkaloids are found in all plant parts like roots, stems, leaves, flowers, fruits and seeds. in the present study, those plant parts of solanum dulcamara were selected which have been reported to produce a high content of a specific alkaloid: solanine (from unripe fruits), solasodine ... | 2009 | 19418354 |
| how beta-lactam antibiotics enter bacteria: a dialogue with the porins. | multi-drug resistant (mdr) infections have become a major concern in hospitals worldwide. this study investigates membrane translocation, which is the first step required for drug action on internal bacterial targets. beta-lactams, a major antibiotic class, use porins to pass through the outer membrane barrier of gram-negative bacteria. clinical reports have linked the mdr phenotype to altered membrane permeability including porin modification and efflux pump expression. | 2009 | 19434239 |
| incidence of multiple antibiotic resistant gram-negative bacteria isolated from surface and underground water sources in south western region of nigeria. | in most rural and urban settlements, particularly in nigeria, wells, spring, streams or rivers and lakes serves as major sources of water supply for drinking and other domestic purposes. unfortunately, many of the available water sources are not potable without some form of treatment which is seldom available in most settings. the use of untreated surface water sources for drinking and for domestic purposes remains a major threat to public health as these could serve as reservoirs the for transf ... | 2009 | 19474486 |
| copper uptake by pseudomonas aeruginosa isolated from infected burn patients. | pseudomonas aeruginosa was isolated from infected burn patients and characterized by standard biochemical tests. the in vitro copper uptake was compared between this isolated pathogenic strain and two non-pathogenic control strains of gram positive bacteria bacillus thuringiensis strain israelis as well as gram negative bacteria enterobacter aerogenes. maximum copper uptake of 470 ppm/g biomass was obtained by p. aeruginosa strain, while the control strains b. thuringiensis and enterobacter aero ... | 2009 | 19484296 |
| role of metal resistant plant growth promoting bacteria in ameliorating fly ash to the growth of brassica juncea. | in this study, we have shown that the plant growth promoting bacterial strain nbri k24 and strain nbri k3 from fly ash (fa) contaminated soil reduce the toxicity of ni and cr in brassica juncea (indian mustard) and promote plant growth under pot culture experiments. isolated strains nbri k24 and nbri k3 were characterized based on the 16s rdna sequencing and identified as enterobacter aerogenes and rahnella aquatilis respectively. both the strains were siderophore producing and found capable of ... | 2009 | 19487076 |
| influence of microbial growth on the redox potential of fermented cucumbers. | commonly, ph measurements are used during the production of fermented cucumbers to indirectly monitor growth of lactic acid bacteria (lab) and acid production. redox potential (e(h)) measurements, which are determined by the potential of an electron to reduce an acceptor, could serve as an alternative tool to monitor the progress of fermentation allowing the detection of the metabolic activity and/or growth of lab and other microorganisms. pasteurized and inoculated jars of cucumbers were observ ... | 2009 | 19490331 |
| sepsis resulting from enterobacter aerogenes resistant to carbapenems after liver transplantation. | sepsis due to enterobacter aerogenes (e. aerogenes) is rare after liver transplantation but is also a serious infection that may cause liver abscess. the purpose of this case report is to relate an unusual presentation of liver transplantation to show how successive treatment can be an appropriate option in septic patients after liver transplantation. | 2009 | 19502176 |
| expression of nad+-dependent formate dehydrogenase in enterobacter aerogenes and its involvement in anaerobic metabolism and h2 production. | an expression system for nad(+)-dependent formate dehydrogenase gene (fdh1), from candida boidinii, was constructed and cloned into enterobacter aerogenes iam1183. with the fdh1 expression, the total h(2) yield was attributed to a decrease in activity of the lactate pathway and an increase of the formate pathway flux due to the nadh regeneration. analysis of the redox state balance and ethanol-to-acetate ratio in the fdhl-expressed strain showed that increased reducing power arose from the recon ... | 2009 | 19533026 |
| [mechanisms of bioelectricity generation in enterobacter aerogenes-based microbial fuel cells]. | microbial fuel cells (mfcs) using hydrogen-producing bacteria (hpb) could utilize a large number of substrates to generate power. however, the coulombic efficiency is limited by the fact that only suspended cells are used as biocatalyst in anodic medium. mfcs using fe (iii)-reducing bacteria have high energy recovery efficiency, but can only utilize some simple organic matters. in this study, enterobacter aerogenes xm02, a hydrogen-producing strain with fe(iii)-reducing activity, was selected as ... | 2009 | 19545032 |
| investigation and control of an outbreak of enterobacter aerogenes bloodstream infection in a neonatal intensive care unit in fiji. | ten neonates developed blood stream infection with extended-spectrum beta-lactamase-producing enterobacter aerogenes in a neonatal intensive care unit in fiji. the source of the outbreak was traced to a bag of contaminated normal saline in the ward, which was used for multiple patients. all isolates recovered from patients were indistinguishable from the bacteria recovered from the normal saline by pulsed-field gel electrophoresis. the outbreak was controlled using simple infection control pract ... | 2009 | 19552517 |
| synthesis, nematicidal and antimicrobial properties of bis-[4-methoxy-3-[3-(4-fluorophenyl)-6-(4-methylphenyl)-2(aryl)-tetrahydro-2h-pyrazolo[3,4-d]thiazol-5-yl]phenyl]methanes. | a new series of bis-[4-methoxy-3-[3-(4-fluorophenyl)-6-(4-methylphenyl)-2-(aryl)-3,3a,5,6-tetrahydro-2h-pyrazolo[3,4-d][1,3]thiazol-5-yl]phenyl]methanes 6a-r was synthesized by the reaction of arylidine derivative of methylene-bis-thiazolidinones 5a-c with aryl/alkyl hydrazines. chemical structures of all the new compounds were established by ir, (1)h-nmr, (13)c-nmr, ms and elemental data. the compounds 6a-r were evaluated for their nematicidal activity against ditylenchus myceliophagus and caen ... | 2009 | 19571412 |
| combining capillary electrophoresis with laser-induced fluorescence detection for the analysis of escherichia coli lysates. | with the goal of improving water quality control, in this study we combined ce-lif for the analysis of escherichia coli lysates under discontinuous conditions. prior to injecting a large-volume protein sample, a plug of 0.2% w/v sds was injected into the capillary filled with 2.0 m tris-borate (tb) solution (ph 10.3). during the courses of the process stacking, proteins migrated against the electroosmotic flow (eof) and entered a 1.7% w/v poly(ethylene oxide) solution that has been prepared in 2 ... | 2009 | 19621366 |