| reduced glutathione mediates resistance to h2s toxicity in oral streptococci. | periodontal disease is associated with changes in the composition of the oral microflora, where health-associated oral streptococci decrease while gram-negative anaerobes predominate in disease. a key feature of periodontal disease-associated anaerobes is their ability to produce hydrogen sulfide (h2s) abundantly as a by-product of anaerobic metabolism. so far, h2s has been reported to be either cytoprotective or cytotoxic by modulating bacterial antioxidant defense systems. although oral anaero ... | 2016 | 26801579 |
| systematic engineering of the central metabolism in escherichia coli for effective production of n-butanol. | microbes have been extensively explored for production of environment-friendly fuels and chemicals. the microbial fermentation pathways leading to these commodities usually involve many redox reactions. this makes the fermentative production of highly reduced products challenging, because there is a limited nadh output from glucose catabolism. microbial production of n-butanol apparently represents one typical example. | 2016 | 26997975 |
| oral microbiota species in acute apical endodontic abscesses. | acute apical abscesses are serious endodontic diseases resulting from pulpal infection with opportunistic oral microorganisms. the objective of this study was to identify and compare the oral microbiota in patients (n=18) exhibiting acute apical abscesses, originating from the demographic region in portland, oregon. the study hypothesis is that abscesses obtained from this demographic region may contain unique microorganisms not identified in specimens from other regions. | 2016 | 26983837 |
| microrna expression profile of human periodontal ligament cells under the influence of porphyromonas gingivalis lps. | periodontitis is a chronic inflammatory disease which is caused by bacterial infection and leads to the destruction of periodontal tissues and resorption of alveolar bone. thus, special attention should be paid to the mechanism under lipopolysaccharide (lps)-induced periodontitis because lps is the major cause of periodontitis. however, to date, mirna expression in the lps-induced periodontitis has not been well characterized. in this study, we investigated mirna expression patterns in lps-treat ... | 2016 | 26987780 |
| genome-wide association study of biologically informed periodontal complex traits offers novel insights into the genetic basis of periodontal disease. | genome-wide association studies (gwas) of chronic periodontitis (cp) defined by clinical criteria alone have had modest success to-date. here, we refine the cp phenotype by supplementing clinical data with biological intermediates of microbial burden (levels of eight periodontal pathogens) and local inflammatory response (gingival crevicular fluid il-1β) and derive periodontal complex traits (pcts) via principal component analysis. pcts were carried forward to gwas (∼2.5 million markers) to iden ... | 2016 | 26962152 |
| microbial diversity in the early in vivo-formed dental biofilm. | although the mature dental biofilm composition is well studied, there is very little information on the earliest phase of in vivo tooth colonization. progress in dental biofilm collection methodologies and techniques of large-scale microbial identification have made new studies in this field of oral biology feasible. the aim of this study was to characterize the temporal changes and diversity of the cultivable and noncultivable microbes in the early dental biofilm. samples of early dental biofil ... | 2016 | 26746720 |
| antibacterial activity and mechanism of action of auranofin against multi-drug resistant bacterial pathogens. | traditional methods employed to discover new antibiotics are both a time-consuming and financially-taxing venture. this has led researchers to mine existing libraries of clinical molecules in order to repurpose old drugs for new applications (as antimicrobials). such an effort led to the discovery of auranofin, a drug initially approved as an anti-rheumatic agent, which also possesses potent antibacterial activity in a clinically achievable range. the present study demonstrates auranofin's antib ... | 2016 | 26936660 |
| hand to mouth: a systematic review and meta-analysis of the association between rheumatoid arthritis and periodontitis. | rheumatoid arthritis (ra) and periodontitis are both chronic inflammatory diseases, which demonstrate similarities in terms of mechanism, histopathology, and demography. an association between these conditions has been demonstrated previously but has been called into question more recently. | 2016 | 26973655 |
| detection of cfxa2, cfxa3, and cfxa6 genes in beta-lactamase producing oral anaerobes. | purpose the aim of this study was to identify β-lactamase-producing oral anaerobic bacteria and screen them for the presence of cfxa and blatem genes that are responsible for β-lactamase production and resistance to β-lactam antibiotics. material and methods periodontal pocket debris samples were collected from 48 patients with chronic periodontitis and anaerobically cultured on blood agar plates with and without β-lactam antibiotics. presumptive β-lactamase-producing isolates were evaluated for ... | 2016 | 27119762 |
| comparison of the efficacy of subgingival irrigation with 2% povidone-iodine and tetracycline hcl in subjects with chronic moderate periodontitis: a clinico microbiological study. | this study was performed to evaluate and compare the clinical and antimicrobial efficacy of subgingival irrigation with tetracycline and povidone-iodine as an adjunct to nonsurgical periodontal therapy. | 2016 | 27076823 |
| microbiological and clinical effects of sitafloxacin and azithromycin in periodontitis patients receiving supportive periodontal therapy. | sitafloxacin (stfx) is a newly developed quinolone that has robust antimicrobial activity against periodontopathic bacteria. we previously reported that oral administration of stfx during supportive periodontal therapy was as effective as conventional mechanical debridement under local anesthesia microbiologically and clinically for 3 months. the aim of the present study was to examine the short-term and long-term microbiological and clinical effects of systemic stfx and azithromycin (azm) on ac ... | 2016 | 26729495 |
| neutrophil functions in periodontal homeostasis. | oral tissues are constantly exposed to damage from the mechanical effort of eating and to microorganisms, mostly bacteria. in healthy gingiva tissue remodeling and a balance between bacteria and innate immune cells are maintained. however, excess of bacteria biofilm (plaque) creates an inflammation state that recruits more immune cells, mainly neutrophils to the gingiva. neutrophils create a barrier for bacteria to reach inside tissues. when neutrophils are insufficient, bacteria thrive causing ... | 2016 | 27019855 |
| management of diabolical diabetes mellitus and periodontitis nexus: are we doing enough? | periodontitis is the commonest oral disease affecting population worldwide. this disease is notorious for the devastation of tooth supporting structures, ensuing in the loss of dentition. the etiology for this disease is bacterial biofilm, which accumulates on the teeth as dental plaque. in addition to the biofilm microorganisms, other factors such as environmental, systemic and genetic are also responsible in progression of periodontitis. diabetes mellitus (dm) is metabolic disorder which has a ... | 2016 | 26962409 |
| n-butanol production in saccharomyces cerevisiae is limited by the availability of coenzyme a and cytosolic acetyl-coa. | butanol isomers are regarded as more suitable fuel substitutes than bioethanol. n-butanol is naturally produced by some clostridia species, but due to inherent problems with clostridial fermentations, industrially more relevant organisms have been genetically engineered for n-butanol production. although the yeast saccharomyces cerevisiae holds significant advantages in terms of scalable industrial fermentation, n-butanol yields and titers obtained so far are only low. | 2016 | 26913077 |
| bacterial diversity in saliva and oral health-related conditions: the hisayama study. | this population-based study determined the salivary microbiota composition of 2,343 adult residents of hisayama town, japan, using 16s rrna gene next-generation high-throughput sequencing. of 550 identified species-level operational taxonomic units (otus), 72 were common, in ≥75% of all individuals, as well as in ≥75% of the individuals in the lowest quintile of phylogenetic diversity (pd). these "core" otus constituted 90.9 ± 6.1% of each microbiome. the relative abundance profiles of 22 of the ... | 2016 | 26907866 |
| identification of known and novel recurrent viral sequences in data from multiple patients and multiple cancers. | virus discovery from high throughput sequencing data often follows a bottom-up approach where taxonomic annotation takes place prior to association to disease. albeit effective in some cases, the approach fails to detect novel pathogens and remote variants not present in reference databases. we have developed a species independent pipeline that utilises sequence clustering for the identification of nucleotide sequences that co-occur across multiple sequencing data instances. we applied the workf ... | 2016 | 26907326 |
| influence of type 2 diabetes on prevalence of key periodontal pathogens, salivary matrix metalloproteinases, and bone remodeling markers in sudanese adults with and without chronic periodontitis. | this study compared the influence of type 2 diabetes on the occurrence of six periodontal pathogens in plaque samples of patients with and without chronic periodontitis. levels of salivary mmp-8, mmp-9, rankl, and opg were also investigated. the study enrolled 31 patients with type 2 diabetes and chronic periodontitis (dm + cp), 29 with chronic periodontitis (cp), and 20 with type 2 diabetes (dm). questionnaire-guided interviews were conducted and plaque index, bleeding on probing, and pocket de ... | 2016 | 26989414 |
| prevalence of porphyromonas gingivalis fima genotypes in the peri-implant sulcus of koreans assessed using a new primer. | porphyromonas gingivalis fima is a virulence factor associated with periodontal diseases, but its role in the pathogenesis of peri-implantitis remains unclear. we aimed to evaluate the relationship between the condition of peri-implant tissue and the distribution of p. gingivalis fima genotypes in koreans using a new primer. | 2016 | 26937292 |
| surface interactions between two of the main periodontal pathogens: porphyromonas gingivalis and tannerella forsythia. | porphyromonas gingivalis and tannerella forsythia have been implicated as the major etiologic agents of periodontal disease. these two bacteria are frequently isolated together from the periodontal lesion, and it has been suggested that their interaction may increase each one's virulence potential. the purpose of this study was to identify proteins on the surface of these organisms that are involved in interbacterial binding. | 2016 | 26937289 |
| role of nitric oxide in the pathogenesis of barrett's-associated carcinogenesis. | barrett's esophagus (be), a premalignant condition to barrett's adenocarcinoma (bac), is closely associated with chronic inflammation due to gastro-esophageal reflux. caudal type homeobox 2 (cdx2), a representative marker of be, is increased during the metaplastic and neoplastic transformation of be. nitric oxide (no) has been proposed to be a crucial mediator of barrett's carcinogenesis. we previously demonstrated that cdx2 might be induced directly under stimulation of large amounts of no gene ... | 2016 | 26909236 |
| porphyromonas gingivalis: an overview of periodontopathic pathogen below the gum line. | periodontal disease represents a group of oral inflammatory infections initiated by oral pathogens which exist as a complex biofilms on the tooth surface and cause destruction to tooth supporting tissues. the severity of this disease ranges from mild and reversible inflammation of the gingiva (gingivitis) to chronic destruction of connective tissues, the formation of periodontal pocket and ultimately result in loss of teeth. while human subgingival plaque harbors more than 500 bacterial species, ... | 2016 | 26903954 |
| pyrf as a counterselectable marker for unmarked genetic manipulations in treponema denticola. | the pathophysiology of treponema denticola, an oral pathogen associated with both periodontal and endodontic infections, is poorly understood due to its fastidious growth and recalcitrance to genetic manipulations. counterselectable markers are instrumental in constructing clean and unmarked mutations in bacteria. here, we demonstrate that pyrf, a gene encoding orotidine-5'-monophosphate decarboxylase, can be used as a counterselectable marker in t. denticola to construct marker-free mutants. t. ... | 2016 | 26682856 |
| dysbiosis may trigger autoimmune diseases via inappropriate post-translational modification of host proteins. | the gut ecosystem with myriads of microorganisms and the high concentration of immune system cells can be considered as a separate organ on its own. the balanced interaction between the host and microbial cells has been shaped during the long co-evolutionary process. in dysbiotic conditions, however, this balance is compromised and results in abnormal interaction between the host and microbiota. it is hypothesize here that the changed spectrum of microbial enzymes involved in post-translational ... | 2016 | 26903965 |
| periodontal disease's contribution to alzheimer's disease progression in down syndrome. | people with down syndrome (ds) are at an increased risk for alzheimer's disease (ad). after 60 years of age, >50% of ds subjects acquire dementia. nevertheless, the age of onset is highly variable possibly because of both genetic and environmental factors. genetics cannot be modified, but environmental risk factors present a potentially relevant intervention for ds persons at risk for ad. among them, inflammation, important in ad of ds type, is potential target. consistent with this hypothesis, ... | 2016 | 27239536 |
| long-term oil contamination alters the molecular ecological networks of soil microbial functional genes. | with knowledge on microbial composition and diversity, investigation of within-community interactions is a further step to elucidate microbial ecological functions, such as the biodegradation of hazardous contaminants. in this work, microbial functional molecular ecological networks were studied in both contaminated and uncontaminated soils to determine the possible influences of oil contamination on microbial interactions and potential functions. soil samples were obtained from an oil-exploring ... | 2016 | 26870020 |
| management of peri-implantitis: a systematic review, 2010-2015. | peri-implantitis or periimplantitis is characterized as an inflammatory reaction that affects the hard and soft tissue, which results in loss of supporting bone and pocket formation surrounding the functioning osseointegrated implant. this review aimed to evaluate the effectiveness of surgical and non-surgical treatment of peri-implantitis. the data sources used was pubmed. searches of this database were restricted to english language publications from january 2010 to june 2015. all randomized c ... | 2016 | 26877903 |
| nutrients, microglia aging, and brain aging. | as the life expectancy continues to increase, the cognitive decline associated with alzheimer's disease (ad) becomes a big major issue in the world. after cellular activation upon systemic inflammation, microglia, the resident immune cells in the brain, start to release proinflammatory mediators to trigger neuroinflammation. we have found that chronic systemic inflammatory challenges induce differential age-dependent microglial responses, which are in line with the impairment of learning and mem ... | 2016 | 26941889 |
| interleukin-17a gene variability in patients with type 1 diabetes mellitus and chronic periodontitis: its correlation with il-17 levels and the occurrence of periodontopathic bacteria. | interleukin-17 contributes to the pathogenesis of type 1 diabetes mellitus (t1dm) and chronic periodontitis (cp). we analyzed il-17a -197a/g and il-17f +7488c/t polymorphisms in t1dm and cp and determined their associations with il-17 production and occurrence of periopathogens. totally 154 controls, 125 t1dm, and 244 cp patients were genotyped using 5' nuclease taqman(®) assays. bacterial colonization was investigated by a dna-microarray kit. production of il-17 after in vitro stimulation of mo ... | 2016 | 26924897 |
| comparative genomics reveals new evolutionary and ecological patterns of selenium utilization in bacteria. | selenium (se) is an important micronutrient for many organisms, which is required for the biosynthesis of selenocysteine, selenouridine and se-containing cofactor. several key genes involved in different se utilization traits have been characterized; however, systematic studies on the evolution and ecological niches of se utilization are very limited. here, we analyzed more than 5200 sequenced organisms to examine the occurrence patterns of all se traits in bacteria. a global species map of all ... | 2016 | 26800233 |
| helicobacter pylori colonization of the oral cavity: a milestone discovery. | over the past several years, the severity of helicobacter pylori (h. pylori) infections has not significantly diminished. after successful eradication, the annual h. pylori recurrence rate is approximately 13% due to oral h. pylori infection. established clinical diagnostic techniques do not identify an oral etiologic basis of h. pylori prior to gastric infection. there has been disagreement as to whether oral infection of h. pylori exists or not, with no definite conclusion. in medical practice ... | 2016 | 26811613 |
| a comprehensive benchmarking study of protocols and sequencing platforms for 16s rrna community profiling. | in the last 5 years, the rapid pace of innovations and improvements in sequencing technologies has completely changed the landscape of metagenomic and metagenetic experiments. therefore, it is critical to benchmark the various methodologies for interrogating the composition of microbial communities, so that we can assess their strengths and limitations. the most common phylogenetic marker for microbial community diversity studies is the 16s ribosomal rna gene and in the last 10 years the field h ... | 2016 | 26763898 |
| the rise of crispr/cas for genome editing in stem cells. | genetic manipulation is a powerful tool to establish the causal relationship between a genetic lesion and a particular pathological phenotype. the rise of crispr/cas9 genome-engineering tools overcame the traditional technical bottleneck for routine site-specific genetic manipulation in cells. to create the perfect in vitro cell model, there is significant interest from the stem cell research community to adopt this fast evolving technology. this review addresses this need directly by providing ... | 2016 | 26880991 |
| impact of denture cleaning method and overnight storage condition on denture biofilm mass and composition: a cross-over randomized clinical trial. | appropriate oral hygiene is required to maintain oral health in denture wearers. this study aims to compare the role of denture cleaning methods in combination with overnight storage conditions on biofilm mass and composition on acrylic removable dentures. | 2016 | 26730967 |
| quantitative target analysis and kinetic profiling of acyl-coas reveal the rate-limiting step in cyanobacterial 1-butanol production. | cyanobacterial 1-butanol production is an important model system for direct conversion of co2 to fuels and chemicals. metabolically-engineered cyanobacteria introduced with a heterologous coenzyme a (coa)-dependent pathway modified from clostridium species can convert atmospheric co2 into 1-butanol. efforts to optimize the 1-butanol pathway in synechococcus elongatus pcc 7942 have focused on the improvement of the coa-dependent pathway thus, probing the in vivo metabolic state of the coa-depende ... | 2016 | 26766939 |
| therapeutic targets for management of periodontitis and diabetes. | the increasing incidence of diabetes mellitus (dm) and chronic periodontitis (cp) worldwide imposes a rethinking of individualized therapy for patients with both conditions. central to bidirectional links between dm and cp is deregulated systemic inflammation and dysfunctional immune responses to altered-self and non-self. control of blood glucose levels and metabolic imbalances associated with hyperglycemia in dm, and disruption of pathogenic subgingival biofilms in cp are currently the main th ... | 2016 | 26881443 |
| biofilms as "connectors" for oral and systems medicine: a new opportunity for biomarkers, molecular targets, and bacterial eradication. | oral health and systems medicine are intimately related but have remained, sadly, as isolated knowledge communities for decades. are there veritable connector knowledge domains that can usefully link them together on the critical path to biomarker research and "one health"? in this context, it is noteworthy that bacteria form surface-attached communities on most biological surfaces, including the oral cavity. biofilm-forming bacteria contribute to periodontal diseases and recent evidences point ... | 2016 | 26583256 |
| toll-like receptors in the vascular system: sensing the dangers within. | toll-like receptors (tlrs) are components of the innate immune system that respond to exogenous infectious ligands (pathogen-associated molecular patterns, pamps) and endogenous molecules that are released during host tissue injury/death (damage-associated molecular patterns, damps). interaction of tlrs with their ligands leads to activation of downstream signaling pathways that induce an immune response by producing inflammatory cytokines, type i interferons (ifn), and other inflammatory mediat ... | 2016 | 26721702 |
| in vitro effect of porphyromonas gingivalis methionine gamma lyase on biofilm composition and oral inflammatory response. | methanethiol (methyl mercaptan) is an important contributor to oral malodour and periodontal tissue destruction. porphyromonas gingivalis, prevotella intermedia and fusobacterium nucleatum are key oral microbial species that produce methanethiol via methionine gamma lyase (mgl) activity. the aim of this study was to compare an mgl knockout strain of p. gingivalis with its wild type using a 10-species biofilm co-culture model with oral keratinocytes and its effect on biofilm composition and infla ... | 2016 | 28033374 |
| assessing the progression of chronic periodontitis using subgingival pathogen levels: a 24-month prospective multicenter cohort study. | the diagnosis of the progression of periodontitis presently depends on the use of clinical symptoms (such as attachment loss) and radiographic imaging. the aim of the multicenter study described here was to evaluate the diagnostic use of the bacterial content of subgingival plaque recovered from the deepest pockets in assessing disease progression in chronic periodontitis patients. | 2017 | 28093069 |
| 16s rdna-based metagenomic analysis of dental plaque and lung bacteria in patients with severe acute exacerbations of chronic obstructive pulmonary disease. | acute exacerbations of chronic obstructive pulmonary disease (ae-copd) are leading causes of mortality in hospital intensive care units. we sought to determine whether dental plaque biofilms might harbor pathogenic bacteria that can eventually cause lung infections in patients with severe ae-copd. | 2014 | 24484531 |
| the association between detectable plasmatic human immunodeficiency virus (hiv) viral load and different subgingival microorganisms in brazilian adults with hiv: a multilevel analysis. | this study investigates the association between detectable plasmatic human immunodeficiency virus (hiv) viral load (hvl) and high levels of periodontal- and non-periodontal-related microorganisms in the subgingival microbiota of individuals with hiv. | 2014 | 23952074 |
| adjunctive azithromycin in the treatment of aggressive periodontitis: microbiological findings of a 12-month randomized clinical trial. | to compare the subgingival microbiological outcomes of azithromycin or placebo as adjuncts to scaling and root planing (srp) in the treatment of aggressive periodontitis (agp), and to secondarily evaluate the microbiological effect of supragingival scaling in agp patients. | 2012 | 22445846 |
| analysis of the expression of nlrp3 and aim2 in periapical lesions with apical periodontitis and microbial analysis outside the apical segment of teeth. | to detect the distribution and expression levels of the nucleotide-binding oligomerization domain-like receptor protein 3 (nlrp3) and the absent in melanoma 2 (aim2) inflammasomes in periapical lesions and to analyse the possible microbial stimuli outside of teeth. | 2017 | 28193569 |
| oral bacteria in placental tissues: increased molecular detection in pregnant periodontitis patients. | the objective of this study was to identify the dna of oral bacteria in placental samples from women with and without periodontitis who had or had not had preterm births and/or low birthweight (pb/lbw) neonates. | 2015 | 26259070 |
| periodontal therapy effects on nitrite related to oral bacteria: a 6-month randomized clinical trial. | nitrite is a biologic factor relevant to oral and systemic homeostasis. through an oral bacteria reduction process, it was suggested that periodontal therapy and chlorhexidine (chx) rinse could affect nitrite levels, leading to negative effects, such as an increase in blood pressure. this 6-month randomized clinical trial evaluated the effects of periodontal therapeutic protocols on salivary nitrite and its relation to subgingival bacteria. | 2015 | 25811847 |
| microbial dynamics during conversion from supragingival to subgingival biofilms in an in vitro model. | the development of dental caries and periodontal diseases result from distinct shifts in the microbiota of the tooth-associated biofilm. this in vitro study aimed to investigate changes in biofilm composition and structure, during the shift from a 'supragingival' aerobic profile to a 'subgingival' anaerobic profile. biofilms consisting of actinomyces oris, candida albicans, fusobacterium nucleatum, streptococcus oralis, streptococcus mutans and veillonella dispar were aerobically grown in saliva ... | 2016 | 26033167 |
| colonisation of gingival epithelia by subgingival biofilms in vitro: role of "red complex" bacteria. | biofilm formation on tooth surface results in colonisation and invasion of the juxtaposed gingival tissue, eliciting strong inflammatory responses that lead to periodontal disease. this in vitro study investigated the colonisation of human gingival multi-layered epithelium by multi-species subgingival biofilms, and evaluated the relative effects of the "red complex" species (porphyromonas gingivalis, tannerella forsythia and treponema denticola). | 2014 | 24949828 |
| arthritis-induced alveolar bone loss is associated with changes in the composition of oral microbiota. | rheumatoid arthritis (ra) and periodontitis (pd) are chronic inflammatory disorders that cause bone loss. pd tends to be more prevalent and severe in ra patients. previous experimental studies demonstrated that ra triggers alveolar bone loss similarly to pd. the aim of this study was to investigate if arthritis-induced alveolar bone loss is associated with modification in the oral microbiota. checkerboard dna-dna hybridization was employed to analyze forty oral bacterial species in 3 groups of c ... | 2016 | 26996070 |
| influence of highly-active antiretroviral therapy on the subgingival biofilm in hiv-infected patients. | highly-active antiretroviral therapy (haart) has been associated with alterations in subgingival biofilm and periodontal disease. the purpose of the present study was to investigate the association between different haart regimens and the prevalence of periodontal pathogens in hiv-infected patients with chronic periodontitis. | 2016 | 25283543 |
| microbial analysis of subgingival plaque samples compared to that of whole saliva in patients with periodontitis. | the detection of special bacterial species in patients with periodontitis is considered to be useful for clinical diagnosis and treatment. the collection of subgingival plaque samples is the common way for the determination of periodontopathic bacteria. however, recently, salivary analysis has been discussed as an advantageous future diagnostic method for periodontitis because it offers simple quantitative sampling and the possibility to assess various bacteria. the aim of this cross-sectional s ... | 2014 | 24144271 |
| butyrate production under aerobic growth conditions by engineered escherichia coli. | butyrate is an important industrial platform chemical. although several groups have reported butyrate production under oxygen-limited conditions by a native producer, clostridium tyrobutylicum, and by a metabolically engineered escherichia coli, efforts to produce butyrate under aerobic growth conditions have met limited success. here, we constructed a novel butyrate synthetic pathway that functions under aerobic growth conditions in e. coli, by modifying the 1-butanol synthetic pathway reported ... | 2017 | 28089378 |
| construction of coa-dependent 1-butanol synthetic pathway functions under aerobic conditions in escherichia coli. | 1-butanol is an important industrial platform chemical and an advanced biofuel. while various groups have attempted to construct synthetic pathways for 1-butanol production, efforts to construct a pathway that functions under aerobic conditions have met with limited success. here, we constructed a coa-dependent 1-butanol synthetic pathway that functions under aerobic conditions in escherichia coli, by expanding the previously reported (r)-1,3-butanediol synthetic pathway. the pathway consists of ... | 2015 | 25865277 |
| generalized aggressive periodontitis: microbiological composition and clinical parameters in non-surgical therapy. | the aim of this study was to determine the variations in periodontal parameters and microbiological composition in periodontal pockets at the baseline and 3 and 6 months post treatmentin patients with generalized aggressive periodontitis(gap) undergoing non surgical periodontal treatment combined with chlorhexidine and systemic antibiotics. medical and dental history was taken from 10 subjects, average age 30.6±2.7 years, diagnosed with gap. a non surgical periodontal treatment combined with 0.1 ... | 2016 | 28383606 |
| reproducibility of a commercially available subgingival plaque sampling strategy and analysis strategy with oligonucleotide probes. | the aim was to evaluate the intra-test agreement of pooled samples from the deepest periodontal pocket of each quadrant with a commercially available test kit based on hybridization of 16s rrna. | 2017 | 28325127 |
| effects of periodontal treatment on primary sjȫgren's syndrome symptoms. | the aim of this longitudinal prospective study was to evaluate the effects of periodontal treatment on the clinical, microbiological and immunological periodontal parameters, and on the systemic activity (essdai) and subjective (esspri) indexes in patients with primary sjögren's syndrome (pss). twenty-eight female patients were divided into four groups: pss patients with or without chronic periodontitis (scp, sc, respectively), and systemically healthy patients with or without chronic periodonti ... | 2017 | 28099577 |
| secretable small rnas via outer membrane vesicles in periodontal pathogens. | micrornas (mirnas) have been shown to be major regulators of eukaryotic gene expression. however, bacterial rnas comparable in size to eukaryotic mirnas (18-22 nucleotides) have received little attention. recently, a novel class of small rnas similar in size to mirnas (mirna-size, small rnas or msrnas) have also been found in several bacteria. like mirnas, msrnas are approximately 15 to 25 nucleotides in length, and their precursors are predicted to form a hairpin loop secondary structure. here, ... | 2017 | 28068479 |
| high-risk periodontal pathogens contribute to the pathogenesis of atherosclerosis. | periodontal disease (pd) is generated by microorganisms. these microbes can enter the general circulation causing a bacteraemia. the result can be adverse systemic effects, which could promote conditions such as cardiovascular disease. level a evidence supports that pd is independently associated with arterial disease. pd is a common chronic condition affecting the majority of americans 30 years of age and older. atherosclerosis remains the largest cause of death and disability. studies indicate ... | 2017 | 27899684 |
| prevalence of periodontal pathogens as predictor of the evolution of periodontal status. | the aim of this study was to determine the relationship between the prevalence of porphyromonas gingivalis, its fima genotypes, aggregatibacter actinomycetemcomitans, tannerella forsythia, and treponema denticola and the evolution of periodontal health. in a longitudinal prospective study, samples of subgingival plaque were taken from 114 patients (37 with chronic periodontitis, 17 with gingivitis, and 60 periodontally healthy) in the course of a full periodontal examination. pcr was employed to ... | 2016 | 27888369 |
| comparison of two different sampling methods for subgingival plaque: subgingival paper points or mouthrinse sample? | the collection of subgingival plaque samples with paper points is time-consuming and accident-sensitive. however, the collection of saliva is simple and contains pathogens of all intraoral surfaces. the aim of this study is to investigate whether a sampling strategy with mouthrinse (mouthrinse sample [msp]; test) leads to results comparable with standard sampling method (multiple site test from the deepest pocket of each quadrant [mt4]; control). | 2017 | 27786617 |
| association of porphyromonas gingivalis with high levels of stress-induced hormone cortisol in chronic periodontitis patients. | the aim of the present study was to evaluate the association between the occurrence of periodontopathogens with cortisol levels in chronic periodontitis patients. | 2016 | 26194628 |
| a double-blind randomized trial comparing implants with laser-microtextured and machined collar surfaces: microbiologic and clinical results. | the purpose of this study was to compare the clinical outcome of and determine the differences in periodonto-pathogenic microbiota around two types of implant collar surfaces: laser-microtextured (test) vs machined (control). | 2017 | 27632268 |
| the distribution of porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans in patients with alcoholic disease: a pilot study. | both drinking and periodontal disease are serious health and social problems. findings on the effect of alcohol consumption on periodontal disease are inconclusive. | 2016 | 27627556 |
| herpesviral-bacterial co-infection in mandibular third molar pericoronitis. | the aim of this study was to assess the presence of herpesviruses and periodontopathic bacteria and to establish their potential association with pericoronitis. | 2016 | 27620215 |
| effect of helicobacter pylori infection on chronic periodontitis by the change of microecology and inflammation. | helicobacter pylori (h. pylori), a pathogen inducing peptic disease, is recently found to be binding to the progress of periodontitis. most previous studies are case-controlled, and they investigate the risk of h. pylori infection in disease the development of while few studies evaluate the correlation between h. pylori and periodontal pathogens. therefore, we investigated the correlation between h. pylori infection with periodontal parameters, periodontal pathogens and inflammation. the results ... | 2016 | 27602578 |
| longitudinal study on clinical and microbial analysis of periodontal status in pregnancy. | this study was aimed to provide a longitudinal overview of the subgingival bacterial microbiome using fluorescence in situ hybridization (fish) technique, in women in the second trimester of pregnancy (between 14 and 24 weeks), and 48 h and 8 weeks postpartum. of 31 women evaluated during pregnancy, 24 returned for the 48-h and 18 for their 8-week exams postpartum. probing depth (pd), bleeding on probing, clinical attachment level, and presence of calculus were recorded. subgingival plaque sampl ... | 2016 | 27556678 |
| bacterial load of periodontal pathogens among italian patients with chronic periodontitis: a comparative study of three different areas. | the aim of the present study was to evaluate the mean bacterial load of some periodontal pathogenic bacteria in italian patients affected by chronic periodontitis. the sample consisted of 1,762 patients with a clinical diagnosis of chronic periodontitis based on the criteria of the american academy of periodontology sampled in the period 2013-2015; 1,323 patients were from northern italy, 317 from central italy and 122 from southern italy. samples for microbiological analysis were collected from ... | 2016 | 27469562 |
| evaluation of the efficacy of a new oral gel containing carvacrol and thymol for home oral care in the management of chronic periodontitis using pcr analysis: a microbiological pilot study. | the use of chemical devices for domestic oral hygiene in periodontal patients has led to new treatment strategies aiming primarily at a control of infection. over the last few years, carvacrol and thymol (ct) have been subjected to many scientific and medical studies. the purpose of the present study was to assess the effect of ct on the red complex bacteria using polymerase chain reaction (pcr) for microbiological analysis. five patients with a diagnosis of chronic periodontitis in the age grou ... | 2016 | 27469559 |
| [study of mutual dependence of periodontal and colonic microbiome in health and pathology using nsg-sequencing]. | by using ngs-sequencing libraries of dna from periodontal swabs with primers specific to v6 region of 16s rdna prevalence of bacterial genera and species in periodontal and colonic microbiota of patients with periodontitis of different severity and healthy donors was analyzed. hyper-colonization of the colon with akkermansia muciniphila was found to be the most important maker of negative predisposition to periodontitis (t=133,7 at р=10(-6)). this result is in a good agreement with communication ... | 2016 | 27239990 |
| [use of the real-time pcr for study of the periodontal microbiome in patients with combined pathology of gastroduodenal zone and chronic periodontitis]. | the total of 54 patients with chronic periodontitis of different severity was tested using real-time pcr (dentoflor kit). the group included 38 patients with chronic gastritis. for the first time, a higher prevalence of treponema denticola in periodontium of males in comparison with females was demonstrated. the patients with chronic gastritis had more human genome dna at their periodontium than healthy individuals. non-parametric statistical analysis demonstrated high association of periodontiu ... | 2016 | 27183718 |
| detection of five potentially periodontal pathogenic bacteria in peri-implant disease: a comparison of pcr and real-time pcr. | the aim of this study was to compare the microbial analysis methods of polymerase chain reaction (pcr) and real-time pcr (rt-pcr) in terms of detection of five selected potentially periodontal pathogenic bacteria in peri-implant disease. therefore 45 samples of healthy, mucositis and peri-implantitis (n = 15 each) were assessed according to presence of the following bacteria using pcr (dna-strip technology) and rt-pcr (fluorescent dye sybr green-system): aggregatibacter actinomycetemcomitans (aa ... | 2016 | 27142589 |
| quantitative pcr studies of aggregatibacter actinomycetemcomitans and treponema denticola/tanerella forsythensis complex as etiological factors of chronic periodontitis. | real-time quantitative pcr (dentofl or kit) was used to detect dna of periodontal pathogens in specimens from 92 patients with chronic periodontitis and from a control sample of 12 normal subjects. a bimodal distribution of patients by periodontium colonization with a. actinomycetemcomitans, p. gingivalis, p. intermedia, t. forsythensis, and t. denticola was demonstrated. a new approach to interpretation of the results of quantitative evaluation of periodontal pathogens, including the notion of ... | 2016 | 26899837 |
| microbiological distribution of six periodontal pathogens between untreated italian and dutch periodontal patients. | to compare prevalence and microbial load of six periodontal pathogens between italian and dutch patients affected by chronic periodontitis, using oligonucleotide probe technology. | 2016 | 26870849 |
| antibacterial activity of curcumin against periodontopathic bacteria. | curcumin is a polyphenol extracted from root of turmeric and known to possess multifunctional properties, including antibacterial activity. although previous studies have investigated the effects of curcumin on microorganisms, available knowledge on the effects of curcumin on periodontopathic bacteria is still limited. in this study, the antibacterial effect of curcumin on periodontopathic bacteria is investigated, particularly porphyromonas gingivalis. | 2016 | 26447754 |
| protein biomarkers and microbial profiles in peri-implantitis. | the aim of the present investigation was to determine the profile of peri-implant crevicular fluid (picf) biomarkers combined with microbial profiles from implants with healthy peri-implant tissues and peri-implantitis to assess real-time disease activity. | 2016 | 26424287 |
| subgingivally applied minocycline microgranules in subjects with chronic periodontitis: a randomized clinical and microbiological trial. | the aim of this study was to evaluate clinical and microbiological effects of subgingival minocycline microgranules when used as an adjunct to scaling and root planing in subjects with chronic periodontitis. twenty-six non-smoker volunteers participated in the study. four opposite sites, clinically standardized, with bleeding on probing (bop) and pocket depth (pd) ≥ 6 mm were selected. baseline bop, pd and clinical attachment level (cal) were measured and microbiological samples were collected f ... | 2015 | 26355881 |
| the association between subgingival periodontal pathogens and systemic inflammation. | to investigate associations between periodontal disease pathogens and levels of systemic inflammation measured by c-reactive protein (crp). | 2015 | 26309048 |
| clinical and microbiologic evaluation of scaling and root planing per quadrant and one-stage full-mouth disinfection associated with azithromycin or chlorhexidine: a clinical randomized controlled trial. | conflicting data about the protocol of choice for non-surgical periodontal therapy with adjuvant use are still reported. this study aims to evaluate, through clinical and microbiologic parameters, the systemic use of azithromycin (az) and chlorhexidine (chx) as adjuvants to non-surgical periodontal treatment performed by one-stage full-mouth disinfection (fmd) within 24 hours or conventional quadrant scaling (qs) in four weekly sections. | 2015 | 26252751 |
| detection of subgingival periodontal pathogens--comparison of two sampling strategies. | the aim of the study is to compare detection frequency of periodontal pathogens in patients with aggressive/severe chronic periodontitis using pooled plaque samples from the deepest pockets per quadrant/per sextant. | 2016 | 26193958 |
| prevalence of eight putative periodontal pathogens in atherosclerotic plaque of coronary artery disease patients and comparing them with noncardiac subjects: a case-control study. | the study was aimed to assess the prevalence of periodontal pathogens namely tannerella forsythia (t.f), campylobacter rectus (c.r), eikenella corrodens (e.c), porphyromonas gingivalis (p.g), treponema denticola (t.d), prevotella nigrescens (p.n) ,aggregatibacter actinomycetemcomitans (a.a), p.g (fi ma gene) and prevotella intermedia (p.i), in the subgingival and the atheromatous plaque of patients with coronary artery disease (cad), and comparing them with the noncardiac subjects thereby indica ... | 2017 | 26096116 |
| evaluation of a hydrophobic gel adhering to the gingiva in comparison with a standard water-soluble 1% chlorhexidine gel after scaling and root planing in patients with moderate chronic periodontitis. a randomized clinical trial. | to evaluate the clinical, microbiological and enzymatic activity of a hydrophobic chlorhexidine-based gingiva-adhering gel containing herbal ingredients, compared with a commercially available 1% chlorhexidine water-soluble gel, during non-surgical therapy of moderate chronic periodontitis. | 2017 | 26053503 |
| occurrence of periodontal pathogens in ethnic groups from a native brazilian reservation. | the present study was designed to evaluate the occurrence of periodontal pathogens in the subgingival biofilm of 100 native brazilians living at the umutina indian reservation, mato grosso state, brazil. | 2015 | 25847068 |
| antimicrobial photodynamic effect to treat residual pockets in periodontal patients: a randomized controlled clinical trial. | a randomized controlled clinical trial was designed to evaluate the efficacy of the photodynamic therapy (pdt) in the treatment of residual pockets of chronic periodontitis patients. | 2015 | 25808980 |
| a. actinomycetemcomitans profile and red complex bacterial species of an afro-brazilian community: a comparative study. | the primary aim of this cross-sectional study was to compare the levels of red complex bacteria between afro-brazilian and non afro-brazilian cohort. the secondary aim was to compare the distribution of both aggregatibacter actinomycetemcomitans serotype b and its jp2 strains among participants who harboured this bacterial species. | 2015 | 25757149 |
| inter- and intra-test agreement of three commercially available molecular diagnostic tests for the identification of periodontal pathogens. | the aim of the study was to compare the agreement and reproducibility of three test kits (iai pado test 4⋅5®, micro-ident®, and carpegen® perio diagnostik) regarding the detection of porphyromonas gingivalis, tannerella forsythia, treponema denticola, and aggregatibacter actinomycetemcomitans. | 2015 | 25680706 |
| short-term effect of removal of fixed orthodontic appliances on gingival health and subgingival microbiota: a prospective cohort study. | the aim of this prospective longitudinal study was to assess and compare the microbiological and clinical parameters of patients wearing a fixed orthodontic appliance, as opposed to 10 days after the bracket had been removed following treatment. | 2015 | 25631494 |
| periodontopathogen levels following the use of an er:yag laser in the treatment of chronic periodontitis. | inflammatory periodontal diseases are initiated by microbial biofilms. the reduction of the biofilm is important in the management of the disease. this study compares periodontopathogen levels following the treatment of chronic periodontitis using er:yag laser debridement and mechanical scaling and root planing. | 2015 | 25630495 |
| microbial leakage at the implant-abutment connection due to implant insertion maneuvers: cross-sectional study 5 years postloading in healthy patients. | the aim of this study was to test if stress on the prosthetic connection during insertion maneuvers can induce micro-warping at the implant connection. from september 2011 to july 2013, patients with implants loaded for at least 5 years that were placed with 2 different insertion implant mounters-mp (conventional) and me (mountless)-were selected from all of those who had received dental implant therapy in the past and were attending routine check-up or spontaneous visits during the study period ... | 2015 | 25536224 |
| distribution of 8 periodontal microorganisms in family members of chinese patients with aggressive periodontitis. | to date, no information on the distribution of periodontal microorganisms among family members of chinese patients with aggressive peridontitis (agp) is available. the aim of the present study was to investigate the probability of transmission of eight periodontal microorganisms between patients with aggressive periodontitis and their family members. | 2015 | 25526624 |
| microbiological effects of periodontal therapy plus azithromycin in patients with diabetes: results from a randomized clinical trial. | current evidence suggests that periodontal infection may aggravate diabetes control. the aim of this study was to determine the changes in the frequency with which porphyromonas gingivalis, tannerella forsythia, treponema denticola and aggregatibacter actinomycetemcomitans were detected in patients with diabetes with the use of non-surgical therapy plus azithromycin in a randomized clinical trial. one hundred and five (105) patients with diabetes and chronic periodontitis were randomly assigned ... | 2014 | 25523961 |
| detection of periodontal pathogens in the patients with aortic aneurysm. | the occurrence and development of aortic aneurysm (aa) are associated with infection. some researchers have detected the dna of periodontal pathogens in aa samples in certain populations. however, it has not been done in chinese population. the objective of this study was to evaluate the prevalence of periodontal pathogens in oral tissue samples and aneurysm samples of aa patients. | 2014 | 25430459 |
| periodontal and peri-implant microbiota in patients with healthy and inflamed periodontal and peri-implant tissues. | to compare the prevalence and levels of six bacterial pathogens within the subgingival/submucosal microbiota at teeth versus implants with various clinical conditions. | 2016 | 25399962 |
| prevalence of six periodontal pathogens in subgingival samples of italian patients with chronic periodontitis. | the aim of this study was to investigate the subgingival prevalence of six periodontal pathogens in 352 italian patients with chronic periodontitis. possible correlations with clinical parameters, age, gender and smoking status were also investigated. at first visit a pooled subgingival plaque sample was obtained for each subject by using the paper-point method. the samples were processed and analysed according to a commercially available quantitative real time polymerase chain reaction assay (m ... | 2014 | 25387289 |
| effect of non-surgical periodontal treatment on short chain fatty acid levels in gingival crevicular fluid of patients with generalized aggressive periodontitis. | short chain fatty acids (scfas) play important roles in periodontal diseases. however, the concentrations of scfas in gingival crevicular fluid of patients with aggressive periodontitis are not known. the aim of this intervention study was to investigate the influences of non-surgical periodontal therapy on levels of scfas in the gingival crevicular fluid of patients with generalized aggressive periodontitis (g-agp), and analyze the concentrations of scfas in sites with or without the detected p ... | 2014 | 25340203 |
| polymerase chain reaction as a prospect for the early diagnosis and prediction of periodontal diseases in adolescents. | this study was to determine the markers representative of pathogenic periodontal microflora [prevotella intermedia (p.i), tannerella forsythia (t.f) [bacteroides forsythus], treponema denticola (t.d), actinobacillus actinomycetemcomitans (a.a), porphyromonas gingivalis (p.g)] in the dental plaque of adolescents with various degrees of severity of periodontium inflammation. | 2015 | 25266040 |
| periodontopathogens and human β-defensin-2 expression in gingival crevicular fluid from patients with periodontal disease in guangxi, china. | periodontal diseases are often induced by periodontopathogens, which are always exposed to certain innate immune factors in gingival crevicular fluid, including human β-defensin-2 (hbd-2). this study aims to investigate the relationship among periodontopathogens, clinical parameters and hbd-2 expression. | 2015 | 25065393 |
| effect of repeated adjunctive antimicrobial photodynamic therapy on subgingival periodontal pathogens in the treatment of chronic periodontitis. | the aim of this study was to compare the effect of subgingival ultrasonic scaling followed by repeated (three times) antimicrobial photodynamic therapy (pdt), ultrasonic scaling alone (us), and scaling and root planing with hand instruments (srp) for initial periodontal treatment. twenty-seven non-smoking systemically healthy chronic periodontitis patients were included. residual pockets ≥4 mm deep and bleeding on probing were debrided either with srp, us alone, or us followed by a single episod ... | 2015 | 25056413 |
| rare case of generalised aggressive periodontitis in the primary dentition. | generalised aggressive periodontitis (ap) in the prepubescent age is an exceptionally rare disease in the primary dentition of otherwise healthy children. characteristics of ap are gingival inflammation, deep periodontal pockets, bone loss, tooth mobility and even tooth loss. the most common way of treating this disease is the extraction of all the involved primary teeth. | 2014 | 25001360 |
| internal bacterial colonization of implants: association with peri-implant bone loss. | the aim of the present longitudinal study was to investigate bacterial colonization of the internal implant cavity and to evaluate a possible association with peri-implant bone loss. | 2015 | 24861845 |
| relationship between expression of human gingival beta-defensins and levels of periodontopathogens in subgingival plaque. | human beta-defensins (hbds) are a group of antimicrobial peptides important in epithelial innate immunity, and their differential expression is associated with periodontal diseases. the aim of this study was to explore relationships among hbds, total subgingival bacteria and periodontopathogens in healthy subjects and in patients with chronic periodontitis. | 2015 | 24814979 |
| detection and quantification of periodontal pathogens in smokers and never-smokers with chronic periodontitis by real-time polymerase chain reaction. | the purpose of the present investigation is to compare the presence and number of periodontal pathogens in the subgingival microbiota of smokers versus never-smokers with chronic periodontitis and matched probing depths (pds) using real-time polymerase chain reaction (rt-pcr). | 2014 | 24794687 |