| the serotyping of azospirillum spp. by cell-gold immunoblotting. | ten azospirillum strains were serotyped using the method of cell-gold immunoblotting (dot-blot immune-overlay assay). colloidal gold-protein a conjugate was used. antibodies raised against the whole cells showed strain specificity and interacted mainly with carbohydrate antigens on the cell surface. immunological identity for a. brasilense sp 245 and sp 107 strains was found. cell-gold immunoblotting can be recommended for serotyping of a wide variety of bacterial strains. | 1992 | 1398026 |
| heterotrophic sulfur reduction by thermotoga sp. strain fjss3.b1. | thermotoga sp. strain fjss3.b1 was able to reduce sulfur to sulfide when grown on a mineral medium with glucose as the sole carbon and energy source. there was no increase in specific growth yield coupled to sulfur reduction, but the specific growth rate, final growth yield, and tolerance of h2 were all increased in the presence of sulfur. at dissolved h2 concentrations, of 550 to 600 mumol/l (at 77 degrees c) growth was not possible unless sulfur was added. glucose was fermented via the embden- ... | 1992 | 1398039 |
| metabolism of 3-methyldiphenyl ether by sphingomonas sp. ss31. | the bacterium sphingomonas sp. ss31, which was obtained from the diphenyl ether-degrading strain sphingomonas sp. ss3 by an adaptation process, utilized 3-methyldiphenyl ether for growth in addition to diphenyl ether. the initial enzymatic attack onto this compound proceeded by a regioselective, but non-specific dioxygenation at the carbon carrying the ether bridge and the adjacent carbon of the unsubstituted as well as the methyl-substituted aromatic nucleus. upon spontaneous decomposition, the ... | 1992 | 1398042 |
| vectors for generating nested deletions and facilitating subcloning g+c-rich dna between escherichia coli and streptomyces sp. | new multiple cloning sites (mcs), which facilitate the subcloning of g+c-rich dna, were added to puc18, m13mp18, pve616 (a pbr322-derived insertion vector), and the low-copy-number streptomyces vector, pij922. the mcs in these vectors contain sites found infrequently in streptomyces dna, facilitating the exchange of subclones between the vectors. the mcs added to m13mp18 and puc18 was also designed to generate nested deletions within subcloned fragments. | 1992 | 1398085 |
| protein v, a novel type-ii igg receptor from streptococcus sp.: sequence, homologies and putative fc-binding site. | we have cloned and sequenced the fc-receptor-encoding gene, fcrv, from a group g streptococcus. considerable similarity was revealed between the fcrv, fcra76 and m proteins of group a streptococci in their signal sequences and 3' termini, and between the fc-binding regions of fcrv and fcra76. the promoter and terminator regions showed no homology with those of the fcra76 and m protein-encoding genes. the a1-a4 domains of fcrv (protein v) exhibit a heptapeptide repeat motif which is characteristi ... | 1992 | 1398120 |
| graft eosinophilia in lung transplantation. | graft eosinophilia was observed in lung biopsies from nine patients who received lung allografts. five cases were associated with moderate to severe acute cellular rejection and responded well to steroid therapy. in this group the eosinophilia occurred early after transplantation and was associated with an elevated white blood cell count and occasional peripheral blood (one of five cases) and bronchoalveolar lavage (one of five cases) eosinophilia. a second group of four patients had graft eosin ... | 1992 | 1398645 |
| prevention of c3 deposition by capsular polysaccharide is a virulence mechanism of type iii group b streptococci. | strains of type iii group b streptococci isolated from patients with neonatal sepsis are generally resistant to complement-mediated phagocytic killing in the absence of specific antibody. it has been suggested that the resistance of type iii group b streptococci to phagocytosis results from inhibition of alternative-complement-pathway activation by sialic acid residues of the type iii polysaccharide. to better define the relationship between structural features of the type iii capsule and resist ... | 1992 | 1398910 |
| microbiology of secondarily infected diaper dermatitis. | specimens obtained from 67 infants with secondarily infected diaper dermatitis were cultured for aerobic and anaerobic bacteria. bacteria growth was obtained in 58. aerobic facultative bacteria or candida sp. only were present in 28 patients (48%), anaerobic bacteria only in 11 (19%), and mixed anaerobic with aerobic, facultative, or yeast flora was present in 19 (33%). ninety-one bacterial or fungal isolates were recovered (1.6 per specimen), 54 (0.9 per specimen) aerobic or facultative bacteri ... | 1992 | 1399196 |
| salmonella infections in neonatal dairy calves. | to estimate herd prevalence of salmonella spp, fecal specimens were obtained for culture from neonatal calves of 47 ohio dairy herds. of the 452 calves tested, 10 calves from 7 farms were culture-positive. salmonella serotypes isolated were s dublin, s typhimurium, s enteritidis, s agona, s mbandaka, and s montevideo. bulk tank milk filters from these dairies were also submitted for culture. salmonella sp was isolated from 1 of the 50 filters, and 2 calves from this herd were found to be sheddin ... | 1992 | 1399794 |
| anaerobic bacterial infections causing osteomyelitis/arthritis in a dog. | a 3-year-old german shepherd dog was examined for lameness, signs of pain, swelling, a draining fistulous tract, and osteolysis after a dog bite on the left carpus. after failure of the lesion to respond to several antibiotics, peptostreptococcus sp and propionibacterium sp were isolated from swab specimens and then from surgically collected bone and soft tissue specimens. the bone fragments had mild purulent osteomyelitis associated with numerous gram-positive rods and cocci. the dog was succes ... | 1992 | 1399798 |
| glisoprenins, new inhibitors of acyl-coa: cholesterol acyltransferase produced by gliocladium sp. fo-1513. i. production, isolation and physico-chemical and biological properties. | gliocladium sp. fo-1513 was found to produce novel inhibitors of acyl-coa: cholesterol acyltransferase (acat). two active compounds, designated glisoprenins a and b, were isolated from the culture broth of the producing strain by a conventional method. the ic50 values of glisoprenins a and b for acat activity were 46 and 61 microm in an enzyme assay using rat liver microsomes, and 1.2 and 0.57 microm in a j774 macrophage assay, respectively. | 1992 | 1399839 |
| new cyclodepsipeptides, enniatins d, e and f produced by fusarium sp. fo-1305. | new cyclodepsipeptides named enniatins d, e and f were isolated from the culture broth of fusarium sp. fo-1305 as inhibitors of acyl-coa:cholesterol acyltransferase (acat). the respective structures of enniatins d, e and f were determined to be cyclo[d-alpha-hydroxyisovaleryl(d-hiv)-l-n-methylleucinyl(l- me-leu)-d-hiv-l-n-methylvalinyl(l-me-val)-d-hiv-l-me-val], a mixture of cyclo-[d-hiv-l-me-leu-d-hiv-l-n-methylisoleucinyl(l-me-ile)-d-hiv- l-me-val] and cyclo(d-hiv-l-me-ile-d-hiv-l-me-leu-d-hiv ... | 1992 | 1399840 |
| acaterin, a novel inhibitor of acyl-coa: cholesterol acyltransferase produced by pseudomonas sp. a92. | acaterin, a novel inhibitor of acyl-coa: cholesterol acyltransferase (acat), was isolated from a culture broth of pseudomonas sp. a92 by diaion hp-20 column chromatography, solvent extraction and reverse phase hplc. spectroscopic analyses of the compound yielded 3-(1-hydroxyoctyl)-5-methyl-2(5h)-furanone as the proposed structure. in the presence of oxidized low density lipoprotein, acaterin inhibited the synthesis of cholesteryl ester in macrophage j774 by 50% at a concentration of 45 microm. a ... | 1992 | 1399841 |
| anthracycline metabolites from baumycin-producing streptomyces sp. d788. i. isolation of antibiotic-blocked mutants and their characterization. | biosynthetically blocked mutants were obtained from a baumycin-producing streptomyces sp. d788 newly isolated from soil. the first mutant isolated was a baumycin-negative but daunorubicin-accumulating mutant with a loss of 4'-substitution activity, from which all other blocked mutants were successively derived. these included a known 11-deoxydaunorubicin-producing mutant and several new types of mutants which produced mainly 10-carboxy-13-deoxocarminomycin, 10-methoxycarbonyl-13-deoxocarminomyci ... | 1992 | 1399846 |
| isolation and analysis of a novel inducible pectate lyase gene from the phytopathogenic fungus fusarium solani f. sp. pisi (nectria haematococca, mating population vi). | a pectate lyase produced by fusarium solani f. sp. pisi (nectria haematococca, mating population vi) was previously shown to be essential for host infection (m. s. crawford and p. e. kolattukudy, arch. biochem. biophys. 258:196-205, 1987). pectate lyase genes have not been cloned from any phytopathogenic fungi. a gene, designated pela, encoding an inducible pectate lyase was isolated from f. solani f. sp. pisi. a probe was synthesized by polymerase chain reaction with oligonucleotide primers bas ... | 1992 | 1400187 |
| development of thermus-escherichia shuttle vectors and their use for expression of the clostridium thermocellum cela gene in thermus thermophilus. | we describe the self-selection of replication origins of undescribed cryptic plasmids from thermus aquaticus y-vii-51b (atcc 25105) and a thermus sp. strain (atcc 27737) by random insertion of a thermostable kanamycin adenyltransferase cartridge. once selected, these autonomous replication origins were cloned into the escherichia coli vector puc9 or puc19. the bifunctional plasmids were analyzed for their sizes, relationships, and properties as shuttle vectors for thermus-escherichia cloning. se ... | 1992 | 1400194 |
| pulmonary surfactant protein d specifically binds to phosphatidylinositol. | alveolar type ii cells produce and secrete a complex mixture of lipids and proteins called pulmonary surfactant of which phospholipids are the major components. surfactant proteins (sp) a, b, and c interact with phospholipids and are believed to play important roles in alveolar spaces. however, whether surfactant protein d (sp-d) interacts with phospholipids is unknown. in the present study, we examined whether sp-d binds to phospholipids and investigated phospholipid specificities of sp-d bindi ... | 1992 | 1400434 |
| legionella lansingensis sp. nov. isolated from a patient with pneumonia and underlying chronic lymphocytic leukemia. | a legionella-like organism, strain 1677-mi-h, was isolated from the bronchoscopy washings of a patient with pneumonia who had a 2-year history of progressive, chronic lymphocytic leukemia. the growth characteristics, cellular fatty acids, and ubiquinone content of the isolate were consistent with those for legionella spp. the isolate was serologically distinct in the slide agglutination test with absorbed antisera. dna hybridization studies showed that strain 1677-mi-h (atcc 49751) represents a ... | 1992 | 1401005 |
| degradation of endogenous bacterial cell wall polymers by the muralytic enzyme mutanolysin prevents hepatobiliary injury in genetically susceptible rats with experimental intestinal bacterial overgrowth. | jejunal self-filling blind loops with subsequent small bowel bacterial overgrowth (sbbo) induce hepatobiliary injury in genetically susceptible lewis rats. lesions consist of portal tract inflammation, bile duct proliferation, and destruction. to determine the pathogenesis of sbbo-induced hepatobiliary injury, we treated lewis rats with sbbo by using several agents with different mechanisms of activity. buffer treatment, ursodeoxycholic acid, prednisone, methotrexate, and cyclosporin a failed to ... | 1992 | 1401067 |
| new selective medium for isolating clostridium difficile from faeces. | to compare ccfa (cycloserine, cefoxitin fructose agar) with a new selective medium cdmn (containing cysteine hydrochloride, norfloxacin, and moxalactam) for the isolation of clostridium difficile after direct faecal culture. | 1992 | 1401214 |
| community-acquired bacteremia in the elderly: a prospective study of 121 cases. | to describe community-acquired bacteremia in the elderly and correlate clinical and laboratory findings with outcome. | 1992 | 1401689 |
| microbial degradation of propoxur in turfgrass soil. | this study was conducted to determine the degradation rates in turfgrass soil over a 12-month period after a single field application of propoxur and to isolate microorganisms from the soil capable of degrading the insecticide. soil samples were collected from a turfgrass experimental site near fort lauderdale, fl one week before the field application of propoxur, and over a 12-month period after the field application. mineralization rates in surface (0-15 cm depth) and subsurface (15-30 cm dept ... | 1992 | 1401729 |
| expression and characterization of a cdna clone encoding an immunodominant surface glycoprotein of pneumocystis carinii. | most studies of antigens of pneumocystis carinii have focused on an abundant, immunogenic 95- to 140-kda surface glycoprotein referred to as gpa. expression cloning of gpa from p. carinii obtained from ferrets resulted in isolation of colinear fragments of gpa cdna encoding approximately 87 kda of the core protein. northern hybridization detected an abundant, single species of gpa-specific mrna of 3600 nucleotides. southern hybridization revealed gpa-specific bands only in p. carinii-infected lu ... | 1992 | 1402023 |
| [quantitative analyses of the normal throat flora of children with upper respiratory tract infections]. | relationship between the normal throat flora and pathogenic bacteria recovered from the throat in 139 children with upper respiratory tract infections in winter were studied using quantitative analyses. pathogenic bacteria examined include s. pyogenes, h. influenzae, s. aureus, and s. pneumoniae, and the normal floras include alpha-streptococci, gamma-streptococci, neisseria species, and micrococci. children with s. pyogenes in their throats (s. pyogenes group) were examined with anti-streptococ ... | 1992 | 1402117 |
| immunological detection of the oestradiol receptor protein in cell lines derived from the lymphatic system and the haematopoietic system: variability of specific hormone binding in vitro. | extracts of human mcf 7 mammary carcinoma cells, the human lymphoblastoid cell lines aeh 1 and im 9, t-cell derived ccrf cells, hl 60 myeloic leukaemia cells and murine myeloma cells sp 0 and ns i were analysed for immunoreactivity with polyclonal goat antibodies raised against homogeneous preparations of c-terminal fragments (32 kda) of porcine uterine oestradiol receptor (er). whole cells and low speed cytosols were analysed for specific oestradiol-binding activity. ers were enriched from cell ... | 1992 | 1402547 |
| long-chain alcohol and aldehyde dehydrogenase activities in acinetobacter calcoaceticus strain ho1-n. | three alcohol dehydrogenases have been identified in acinetobacter calcoaceticus sp. strain ho1-n: an nad(+)-dependent enzyme and two nadp(+)-dependent enzymes. one of the nadp(+)-dependent alcohol dehydrogenases was partially purified and was specific for long-chain substrates. with tetradecanol as substrate an apparent km value of 5.2 microm was calculated. this enzyme has a pi of 4.5 and a molecular mass of 144 kda. all three alcohol dehydrogenases were constitutively expressed. three aldehyd ... | 1992 | 1402794 |
| tikusnema javaense n. gen., n. sp. (nematoda: acuarioidea) and other nematodes from rattus argentiventer collected in west java, indonesia. | nematodes collected from the ricefield rat, rattus argentiventer (rodentia: muridae), in pusakanagara and sukamandi, west java, indonesia, are reported. tikusnema javaense n. gen., n. sp. (nematoda:acuariidae:seuratiinae) is described from the small intestine. this new genus is distinguished readily from other genera of the subfamily seuratiinae in having 4 strongly protruded cuticular leaves in the posterior cephalic portion and in having a pair of prominent cuticular ornamentations posterior t ... | 1992 | 1403420 |
| host-parasite relationships of longnose dace, rhinichthys cataractae, from the ford river, michigan. | a total of 1,115 longnose dace, rhinichthys cataractae (family cyprinidae), were examined for parasites from may 1983 through october 1986 from 3 localities in the ford river in michigan's upper peninsula. thirteen parasite species (1 monogenea, 2 digenea, 2 cestoda, 4 nematoda, 1 acanthocephala, 3 protozoa) infected dace. the parasite faunas of dace, taxonomically and in species number, were similar between localities. posthodiplostomum minimum minimum, neascus sp., and rhabdochona canadensis w ... | 1992 | 1403425 |
| reduced splash dispersal of bovine parasitic nematodes from cow pats by the dung beetle diastellopalpus quinquedens. | from a thoroughly mixed portion of cattle feces with cooperia sp. eggs, 1-kg artificial pats were placed in 6 buckets containing 6 kg of soil each. ten dung beetles, diastellopalpus quinquedens, were added to each of 3 buckets. the remaining 3 buckets served as controls without beetles. when infective parasite larvae (l3) had developed in the cow pats indoors, the following procedure was followed. during occasions of rainfall each bucket was placed outdoors in the center of a wider and higher co ... | 1992 | 1403426 |
| longitudinal monitoring of humoral antibody in subjects with destructive periodontal diseases. | fifty-one subjects (16-61 years old) with evidence of prior destructive periodontal disease were monitored clinically and immunologically at bi-monthly intervals for up to 5 yr. periodontal disease activity, determined as new attachment loss, was detected in 33 of these subjects. only 4 of 51 subjects failed to show an elevated serum antibody level to any of the 18 subgingival species tested. the antibody level threshold established for periodontally healthy subjects was exceeded most often in d ... | 1992 | 1403580 |
| spontaneous cardiomyopathy and nephropathy in the owl monkey (aotus sp.) in captivity. | clinical and pathologic data were reviewed for 72 owl monkeys that died between january 1987 and may 1990 at the center for reproduction and conservation of nonhuman primates in iquitos, peru. tissue samples from 39 animals were examined. hypertrophic cardiac disease (51% of animals examined), dilative cardiomyopathy (26%), and nephropathy (87%) were the most common diagnoses. the incidence of all three diseases appeared to increase with time in captivity. nephropathy was less severe in colony-b ... | 1992 | 1404337 |
| large atp synthase operon of the red alga antithamnion sp. resembles the corresponding operon in cyanobacteria. | the large plastid atp synthase operon of the multicellular red alga antithamnion sp. was cloned and the sequence of six atpase genes determined. the operon resembles more the one from cyanobacteria than the atp synthase operon of the chloroplast genome. the gene order is atpi, h, g, f, d and a, coding for the atpase subunits a, c, b', b, delta and alpha, respectively. in green plants, the genes atpg and atpd are located in the nucleus. unlike the situation in three published cyanobacterial atp s ... | 1992 | 1404401 |
| human infection by phagicola sp. (trematoda, heterophyidae) in the municipality of registro, são paulo state, brazil. | in 1988, nine cases of human parasitism by phagicola sp. were diagnosed in the municipality of registro (são paulo state, brazil) by stool examinations, in patients who ate raw mullet (mugil sp.). six (66%) of the nine patients suffered from flatulence and four (44%) had diarrhoeal episodes; six (66%) showed slight eosinophilia. on the same occasion, 61 dogs and 11 cats from registro were also submitted to stool examination. only one dog (1.6%) showed phagicola eggs in the stool. all patients we ... | 1992 | 1404559 |
| electrocochleographic recordings in chronic and healed perilymphatic fistula. | this study examined electrocochleographic recordings in 15 guinea pigs with perilymphatic fistulas. a chronic round window fistula was induced in each animal by insertion of a sigmoid stainless steel needle with the lumen intact. in the contralateral control ear, a similar needle was inserted with the lumen crushed. in this way the model compared acute (control group) versus chronic (experimental group) perilymphatic fisulae. electrocochleographic recordings were obtained before, immediately aft ... | 1992 | 1404574 |
| development of a transformation system for the thermophilic fungus talaromyces sp. cl240 based on the use of phleomycin resistance as a dominant selectable marker. | a transformation system for the thermophilic cellulolytic fungus talaromyces sp. cl240 has been developed, using the phleomycin resistance gene from streptoalloteichus hindustanus (sh ble) as a dominant selectable marker. the plasmids (pan8-1 and put720) carrying the sh ble gene under the control of the aspergillus nidulans glyceraldehyde-3-phosphate dehydrogenase (gpd) promoter, allowed selection of phleomycin-resistant transformants. a new promoter sequence cloned from chromosomal dna of trich ... | 1992 | 1406595 |
| cutinase in cryphonectria parasitica, the chestnut blight fungus: suppression of cutinase gene expression in isogenic hypovirulent strains containing double-stranded rnas. | plant-pathogenic fungi produce cutinase, an enzyme required to degrade plant cuticles and facilitate penetration into the host. the absence of cutinase or a decrease in its production has been associated with a decrease in pathogenicity of the fungus. a set of isogenic strains of cryphonectria parasitica, the chestnut blight fungus, was tested for the presence and amounts of cutinase activity. the virulent strain of c. parasitica produced and secreted significantly higher amounts of cutinase tha ... | 1992 | 1406643 |
| protein kinase c activity correlates with the growth rate of malignant gliomas: part ii. effects of glioma mitogens and modulators of protein kinase c. | the proliferation rates of gliomas may be modulated by the protein kinase c (pkc) signal transduction system. the present study was undertaken to further examine the role of pkc system in growth regulation of gliomas in vitro by measurement of pkc activity over various phases of tumor growth and by assessing its potential role as a signal transduction system induced by serum mitogens and the known glioma mitogens epidermal growth factor and fibroblast growth factor. all human glioma lines examin ... | 1992 | 1407458 |
| cellulose-binding domains: potential for purification of complex proteins. | the endoglucanase cena and the exoglucanase cex from cellulomonas fimi each contain a discrete cellulose-binding domain (cbd), at the amino-terminus or carboxyl-terminus respectively. the gene fragment encoding the cbd can be fused to the gene of a protein of interest. using this approach hybrid proteins can be engineered which bind reversibly to cellulose and exhibit the biological activity of the protein partner. alkaline phosphatase (phoa) from escherichia coli, and a beta-glucosidase (abg) f ... | 1992 | 1409557 |
| dna sequence and analysis of a cryptic 4.2-kb plasmid from the filamentous cyanobacterium, plectonema sp. strain pcc 6402. | the 4194-bp plasmid, prf1, from plectonema sp. strain pcc 6402 was completely sequenced and analyzed. seven potential open reading frames were identified. the predicted amino acid sequence of open reading frame c (orf c) had identities of 34, 29, and 25% with rep b from the staphylococcus aureus plasmid, pub110; rep from the bacillus amyloliquefaciens plasmid, pftb14; and protein a from the s. aureus plasmid, pc194, respectively. a 75-amino-acid region conserved in these proteins (rep b, rep, an ... | 1992 | 1409974 |
| a cluster of tick-borne infections: association with military training and asymptomatic infections due to rickettsia rickettsii. | during the spring of 1989, 86 members of a military unit from the state of maryland, usa, participated in two-week-long training manoeuvres in the states of arkansas (location fc) and virginia (location faph). acute febrile illnesses due to infections with two tick-borne pathogens, rickettsia rickettsii and ehrlichia sp., were confirmed serologically in 2 initial cases who were admitted to the hospital. a seroepidemiological investigation among unit members found an additional 17 of 109 individu ... | 1992 | 1412666 |
| exfoliative colpocytology: a method for the diagnosis of capuchin monkey filariasis? | during vaginal fluid examinations (papanicolaou) to study the physiological sexual cycle of cebus sp., abundant dipetalonema gracile microfilariae (110-160 microns x 4-5 microns, without a sheath) were encountered in the genital fluid, but not in peripheral blood. considering the great difficulty in diagnosing this obscure parasitosis, exfoliative colpocytology was found to be an efficient diagnostic. | 1992 | 1413462 |
| [biotransformation of zearalenone]. | the conversion of zearalenone by some strains of microorganisms was investigated. when the selective strains of rhodotorula sp., arthrobacter sp., saccharomyces sp., and candida sp. were incubated by shaking or standing at 28 degrees c for 72 h with an alcoholic solution of zearalenone as the substrate at a concentration of 2-10 mg/ml ethanol (50-100 micrograms/ml medium), it was readily converted to give zearalenols, consisting either mainly of the alpha-isomer (e.g. 96% in case of rhodotorula ... | 1992 | 1413733 |
| surfactant protein-a inhibits lavage-induced surfactant secretion in newborn rabbits. | in vitro experiments with granular pneumocytes suggest that surfactant protein-a (sp-a) inhibits secretion of pulmonary surfactant. we examined whether sp-a inhibits surfactant secretion induced by lung distention during lung lavage. human sp-a was obtained by lung lavage in a patient with pulmonary alveolar proteinosis. after centrifugation of the lavagate, the pellet was repeatedly washed with saline and then extracted with chloroform:methanol. the methanol:saline phase was separated and lyoph ... | 1992 | 1415320 |
| infection and labor. viii. microbial invasion of the amniotic cavity in patients with suspected cervical incompetence: prevalence and clinical significance. | the purpose of this study was to determine the prevalence and clinical significance of microbial invasion of the amniotic cavity in patients presenting with cervical dilatation in the midtrimester of pregnancy. | 1992 | 1415396 |
| importance of basal glucagon in maintaining hepatic glucose production during a prolonged fast in conscious dogs. | we undertook studies in conscious dogs to assess the role of basal glucagon in stimulating glucose production after a 7-day fast. two protocols consisting of a 40-min basal period (-40 to 0 min), and a 180-min test period (0-180 min) were used. during the test period of the first protocol (hormone replacement; n = 4), somatostatin was infused (0.8 micrograms.kg-1.min-1) along with basal intraportal replacement amounts of insulin and glucagon, whereas in the second protocol (glucagon deficiency; ... | 1992 | 1415534 |
| binding and uptake of surfactant protein b by alveolar type ii cells. | surfactant protein b (sp-b, mol wt 9,000, reduced) is a low-molecular-weight hydrophobic protein found in organic extracts of lung surfactant. the interaction of iodinated bovine sp-b (125i-sp-b) and isolated rat alveolar type ii cells was examined. the association of sp-b with the lung cells was time and temperature dependent; type ii cells exhibited time-dependent binding (at 4 degrees c) and uptake (at 37 degrees c) of sp-b. binding of phospholipid-poor 125i-sp-b was linearly related to the e ... | 1992 | 1415558 |
| effects of differing insulin levels on response to equivalent hypoglycemia in conscious dogs. | the aim of this study was to determine if differing concentrations of insulin can modify the counterregulatory response to equivalent hypoglycemia. insulin was infused intraportally into normal 18-h-fasted conscious dogs at 2 (low, n = 6) or 8 mu.kg-1.min-1 (high, n = 7) on separate occasions. this resulted in steady-state arterial insulin levels of 80 +/- 8 and 610 +/- 55 microu/ml, respectively. glucose was infused during the high dose to maintain plasma glucose similar to low (50 +/- 1 mg/dl) ... | 1992 | 1415687 |
| intracellular processing of pulmonary surfactant protein b in an endosomal/lysosomal compartment. | the hydrophobic surfactant protein b (sp-b) is synthesized in alveolar type ii cells as a 40-kda precursor protein that is processed via 39- and 23-kda intermediates to the mature 8-kda size. to determine the site of sp-b processing, the subcellular distribution of the precursor and mature forms of sp-b was investigated on ultrathin cryosections of human lung using two polyclonal antibodies that discriminate between precursor forms and the mature form of sp-b. an antibody against the human lysos ... | 1992 | 1415726 |
| enzyme-linked immunosorbent assay of colostral igg transported into lymph and plasma in neonatal pigs. | amounts of colostral proteins in lymph and plasma were estimated by an enzyme-linked immunosorbent assay (elisa) after infusion of bovine colostrum into the duodenal lumen in nonsuckling neonatal pigs. the rate of immunoglobulin (ig) g transport in lymph of the thoracic duct reached the maximal level (4.7 +/- 1.3 mg.15 min-1.kg body wt-1) within 3 h after the duodenal infusion. the rate of small protein (sp) transport more slowly increased than that of igg. on the contrary, casein remained in th ... | 1992 | 1415811 |
| morphogenetic and functional activity of type ii cells in early fetal rhesus monkey lungs. a comparison between primates and rodents. | to evaluate further the role of type ii alveolar epithelial cells in primate lung development, lungs of fetal (46 to 155 days gestational age [dga]), postnatal, and adult rhesus monkeys were investigated with antibodies against surfactant protein a (sp-a), alcian blue (ab) staining, and periodic acid-schiff (pas) staining with/without alpha-amylase pre-treatment. in adult and postnatal lungs, type ii cells (cuboid shape; large, roundish nucleus) displayed a unique cytoplasmic staining for sp-a. ... | 1992 | 1416101 |
| community acquired urinary tract infections in singapore: a microbiological study. | we studied 214 patients with culture proven community acquired infections. a total of 219 organisms were isolated, of which 68.2% were escherichia coli, 10.1% klebsiella sp and 5.5% proteus mirabilis. gram positive cocci accounted for 10.5% of organisms isolated. the majority of organisms isolated were sensitive to nalidixic acid (81.1%), nitrofurantoin (80.7%), cephalexin (74.7%) and trimethoprim-sulphamethoxazole (65.0%). resistance to ampicillin was common (49.8%), as such the use of ampicill ... | 1992 | 1416785 |
| kluyveromyces piceae sp. nov., a new yeast species isolated from the rhizosphere of picea abies (l.) karst. | two strains of an undescribed species of the genus kluyveromyces were recovered from the rhizosphere of spruce. a description of the new species, kluyveromyces piceae, is given and its classification on basis of ascospore shape, substrate utilization, g+c-content, dna-dna-reassociation data and habitat specificity is discussed. | 1992 | 1416920 |
| the cestodes of foxhounds and foxes in powys, mid-wales. | cestodes were found in 129 of 875 foxhounds from 20 packs in powys, mid-wales, examined by purging in 1983-1988, in the following order of prevalence: taenia hydatigena in 57 hounds (6.51%), echinococcus granulosus in 27 (3.09%), dipylidium caninum in 25 (2.86%), t. ovis in 14 (1.60%), t. pisiformis in 10 (1.14%), t. serialis in five (0.57%) and t. multiceps in four (0.46%). of 197 foxes examined by autopsy, 22 harboured cestodes: two (1.02%) had e. granulosus, nine (4.57%) t. polyacantha, eight ... | 1992 | 1417205 |
| transformation of synthetic pyrethroid insecticides by a thermophilic bacillus sp. | employing a mineral salts medium containing tween 80 as the primary carbon source, a strain of bacillus stearothermophilus was isolated which was able to hydrolyse selected second and third-generation pyrethroids to non-insecticidal products. of a range of pyrethroid insecticides the trans-isomer of permethrin was the most readily transformed by this microbial isolate, whilst flumethrin was the least. 3-phenoxybenzoic acid and the respective halovinyl or haloacid moieties were detected as the ma ... | 1992 | 1417418 |
| necrotic enteritis in turkeys. | three flocks of turkey hens (16,000 each) between 7 and 12 weeks of age experienced outbreaks of necrotic enteritis. necropsy revealed a dilated duodenum and jejunum with mucosal surfaces covered with a diphtheritic membrane. intestinal scrapings showed very few oocysts of eimeria sp. histopathological findings were compatible with necrotic enteritis but with deeper, more severe lesions than in broiler chickens. clostridium perfringens was isolated by anaerobic culture from the intestinal conten ... | 1992 | 1417616 |
| a single-stranded dna binding protein which interacts with sequences within the bovine preprotachykinin promoter: regulation by nerve growth factor. | the production of substance p (sp) and the mrna encoding its precursor preprotachykinin (ppt) is regulated by nerve growth factor (ngf) in dorsal root ganglion neurons. we have shown previously that two regions of the bovine ppt promoter are capable of mediating the induction by ngf of the downstream structural gene in transfected pc12 cells. both regions contained a sequence element, similar to a known transcription factor binding site, which is present in several other ngf-regulated genes. we ... | 1992 | 1417815 |
| incidence and phenotype restriction of lymphoid blt-serine protease granules in spontaneously diabetes prone bb rats compared with a normal rat strain. | in diabetes prone bb rats a relative increase of serine protease (sp)-positive lymphocytes (39.8 +/- 10%) was observed in peripheral blood at the time of diabetes manifestation (dm) compared with non-diabetic healthy sprague dawley control rats (co: 10.3 +/- 4%), with bb rats at age of premanifestation (pm: 14.7 +/- 4%) and beyond age of expected manifestation (non-diabetic animals, nd: 25.2 +/- 4%). similar absolute numbers were found in diabetic bb rats in comparison with sprague dawley rats. ... | 1992 | 1418295 |
| purification and properties of 4-halobenzoate-coenzyme a ligase from pseudomonas sp. cbs3. | the bacterial strain pseudomonas sp. cbs3 possesses a multi component enzyme system which converts 4-chlorobenzoate to 4-hydroxybenzoate. in the first step 4-chlorobenzoate is activated in a coenzyme a, atp and mg(2+)-dependent reaction to 4-chlorobenzoyl-coenzyme a. atp is cleaved thereby into amp and pyrophosphate. the involved 4-chlorobenzoate-coenzyme a ligase was purified to apparent homogeneity by a 6-step purification procedure. the native enzyme had an apparent molecular mass of 115000 d ... | 1992 | 1418673 |
| [effects of furosemide on endocochlear potentials, auditory action potentials and summating potentials and the changes of inner ear pathology]. | guinea pigs were injected with furosemide 50 mg/kg (group a) and 25mg/kg (group b). two minutes after injection, ep of group a decreased to -13.9mv while that of group b decreased to +65 mv. also, ap of group a disappeared, and recovered at 8.5 mins. while ap amplitude of group b decreased to 78%. the sp value of group a changed from -14.5mv to +23.4mv 1 min after injection and returned to negative polarity in 12 min. edema of stria vascularis was observed under light microscope. transmission el ... | 1992 | 1419180 |
| role of the carboxy terminus of polypeptide d1 in the assembly of a functional water-oxidizing manganese cluster in photosystem ii of the cyanobacterium synechocystis sp. pcc 6803: assembly requires a free carboxyl group at c-terminal position 344. | the d1 polypeptide of the photosystem ii (psii) reaction center is synthesized as a precursor polypeptide which is posttranslationally processed at the carboxy terminus. it has been shown in spinach that such processing removes nine amino acids, leaving ala344 as the c-terminal residue [takahashi, m., shiraishi, t., & asada, k. (1988) febs lett. 240, 6-8; takahashi, y., nakane, h., kojima, h., & satoh, k. (1990) plant cell physiol. 31, 273-280]. we show here that processing on the carboxy side o ... | 1992 | 1420199 |
| lipid bilayer surface association of lung surfactant protein sp-b, amphipathic segment detected by flow immunofluorescence. | lung surfactant protein, sp-b, and synthetic amphipathic peptides derived from sp-b were studied in model lung surfactant lipid bilayers by immunofluorescent labeling. liposomes were formed by hydrating a lipid film on the glass viewing port of a temperature controlled flow chamber. membrane associated peptides were detected by epifluorescence optical microscopy of the binding of anti-peptide polyclonal monospecific antibodies and fitc-conjugated secondary antibodies added to buffer contained in ... | 1992 | 1420912 |
| cloning and sequencing of the petbd operon from the cyanobacterium synechococcus sp. pcc 7002. | the genes encoding the photosynthetic cytochrome b6 (petb) and subunit 4 (petd) have been cloned and sequenced from the unicellular, photoheterotrophic, transformable cyanobacterium synechococcus sp. pcc 7002, formerly designated agmenellum quadruplicatum. the gene arrangement was found to be similar to that reported in the cyanobacterium nostoc pcc 7906. the dna and derived protein sequences were compared to chloroplast and the other cyanobacterial sequences. by pulsed-field electrophoresis, th ... | 1992 | 1421151 |
| factorial experiment to determine influence of fish protein and fish oil on serum and liver lipids in rabbits. | rabbits were fed purified diets consisting of casein (ca), fish protein (fp), and soy protein (sp) combined with maxepa oil (me) or corn oil (cn) to determine the effects of dietary protein and lipid sources on serum total, lipoprotein, and hepatic lipid levels. dietary proteins and lipids exerted significant (p < 0.05) separate effects on serum total cholesterol (tc) (p < 0.005), very-low-density lipoprotein cholesterol (vldl-c) (p < 0.001), and high-density lipoprotein cholesterol (hdl-c) (p < ... | 1992 | 1421781 |
| exogenous porcine surfactant (curosurf) is inactivated by monoclonal antibody to the surfactant-associated hydrophobic protein sp-b. | a monoclonal antibody to the surfactant-associated hydrophobic protein sp-b was added at various concentrations to a standard preparation of porcine surfactant (curosurf 10 mg/ml), and surface properties were evaluated with pulsating bubble. retarded adsorption of surfactant was observed at antibody concentrations > or = 0.5 mg/ml and significantly increased values for minimum surface tension were observed at antibody concentrations > or = 1 mg/ml. in vivo effects of the antibody were tested in ... | 1992 | 1421904 |
| modulation of chromium(vi) toxicity by organic and inorganic sulfur species in yeasts from industrial wastes. | two chromium(vi) resistant yeast strains (candida sp. and rhodosporidium sp.) were isolated from industrial wastes. four different yeasts, three from the industrial yeast collection and one of pharmaceutical origin, were also studied in relation to chromate toxicity and its alleviation by sulfur species. the growth of yeasts from industrial wastes was inhibited by 50% by high concentrations of cr(vi): candida sp. by 4 mm cr(vi) and rhodosporidium sp. by 10 mm cr(vi) in sabouraud broth medium. th ... | 1992 | 1421967 |
| [experimental study of preventing liver cirrhosis by using four kinds of chinese herbs]. | 55 rats were divided into six groups; (1) normal control, (2) cirrhosis control, (3-6) chinese herbs groups, 2-6 groups were liver cirrhosis model caused by combined factor dominated by ccl4, and 3-6 groups were given chinese herbs simultaneously until the end of sixth week. radix salviae miltlorrhizae (rsm) were used in group 3, flos carthami (fc) in group 4, radix angelicae sinensis (ras) in group 5, semen persicae (sp) in group 6. the results: in group 2, 7 out of 9 rats developed into cirrho ... | 1992 | 1421979 |
| isomers of a marine diterpene distinguish sublines of human melanoma cells on the basis of apoptosis, cell cycle arrest and differentiation markers. | the action of the marine furanoditerpenes, spongiatriol (sp) and episopongiatriol (esp), were compared in two sublines of human melanoma cells (mm96e and mm96l) derived from the same metastatic lesion. mm96e had higher tyrosinase activity and lower expression of alkaline phosphatase but was otherwise indistinguishable from mm96l. sp and esp treatment of both cell lines for 72 h at cytostatic doses inhibited b8g3 expression and tyrosinase activity but had little effect on the expression of tyrosi ... | 1992 | 1422191 |
| immunosurveillance by t-lymphocytes in pretumoral stages of chemically induced pancreatic carcinogenesis. | cellular immune-response during pancreatic carcinogenesis induced by n-nitroso-bis(2-hydroxy-propyl)amine in syrian golden hamsters was studied using a mouse antiserum to hamster t-lymphocytes in indirect immunofluorescence. the chronology of lesions in this model is, acinar cell atypia, cystadenoma, ductal hyperplasia and adenocarcinoma. lymphocyte infiltration began before microscopic lesions. starting as an interstitial and interlobular migration, this earliest population was composed of vari ... | 1992 | 1423248 |
| reduction of calcitonin gene-related peptide in jugular blood following electrical stimulation of rat greater occipital nerve. | although it is known that pain in the forehead may be induced by neck abnormalities, the actual neck-head connections responsible for development of pain in trigeminal areas are poorly understood. vasoactive neuropeptides released from sensory fibres, such as substance p (sp) and calcitonin gene-related peptide (cgrp), have been considered as important elements in headache pathophysiology. the levels of cgrp-like immunoreactivity (li) were measured bilaterally in the jugular blood (52 rats) and ... | 1992 | 1423557 |
| regional localization of the gene for clusterin (sp-40,40; gene symbol cli) to human chromosome 8p12-->p21. | the human clusterin (sp-40,40) gene, designated cli (complement lysis inhibitor) by the human gene nomenclature committee, has previously been assigned to chromosome 8. in situ hybridization allowed us to map the locus at 8p12-->p21. | 1992 | 1424805 |
| neuropeptides in pulmonary edema fluid of adult respiratory distress syndrome. | a role for peptidergic nerves in the adult respiratory distress syndrome (ards) was examined by radioimmunochemically quantifying neuropeptides in pulmonary edema (pe) fluids from seven patients with ards and six patients with pe from congestive heart failure (chf). the pe fluid mean concentrations of substance p (sp) and gastrin-releasing peptide (grp) were significantly higher in ards (0.59 +/- 0.29 sd and 0.10 +/- 0.03 nm, respectively, p < 0.001 for both) than in chf (0.19 +/- 0.08 and 0.04 ... | 1992 | 1428125 |
| bronchial challenge with purified protein derivative of mycobacterium tuberculosis in asthma. | response to bronchial challenge (bc) with purified protein derivative of mycobacterium tuberculosis (ppd), was studied in children with bronchial asthma and correlated with mantoux test and serum immunoglobulin e (ige) against ppd (ppd-sp ige). nearly 59% patients with bronchial asthma and 12.2% patients with pulmonary tuberculosis gave positive bc. children with extra-pulmonary tuberculosis and normal children did not show positive bc. in asthma, 25% gave early (ear), 50% gave late (lar) and 25 ... | 1992 | 1428135 |
| effect of air temperature and humidity on ingestive behaviour of sheep. | thirty-two polwarth ewes, of ages up to 1 year, were observed in a climatic chamber (24 to 45 degrees c) for eight periods of 5 h each. the observations were made through a window in the chamber wall. all animals were observed four times, then shorn and observed four times again. the animals were given weighed quantities of water and feed consisting of commercial concentrate plus rhodes grass (chloris gayana) hay. the water and feed remaining after 5 h of observation were weighed. the following ... | 1992 | 1428223 |
| gram-negative bacterial infection in neonatal new world camelids: six cases (1985-1991). | gram-negative bacterial infections were documented in 6 neonatal new world camelids (5 ilamas and 1 alpaca). the organisms isolated from blood before death or from multiple organs after death were escherichia coli (n = 3), actinobacillus sp (n = 1), and klebsiella pneumoniae (n = 1). only 2 crias survived, and 1 became blind secondary to retinal detachment and ocular inflammation, which developed after treatment for bacterial infection. abnormal events during the perinatal period (prematurity, d ... | 1992 | 1429194 |
| benastatins a and b, new inhibitors of glutathione s-transferase, produced by streptomyces sp. mi384-df12. i. taxonomy, production, isolation, physico-chemical properties and biological activities. | benastatins have been isolated as part of a program designed to find microorganism-produced inhibitors of glutathione s-transferase from streptomyces sp. mi384-df12. they were purified by chromatography of reversed-phase silica gel, silica gel and capcell pak c18 (hplc) followed by solvent extraction and then isolated as yellow powders. benastatins a and b have the molecular formulae, c30h28o7 and c30h30o7, respectively. they were competitive with 3,4-dichloronitrobenzene as the substrate, and t ... | 1992 | 1429222 |
| benastatins a and b, new inhibitors of glutathione s-transferase, produced by streptomyces sp. mi384-df12. ii. structure determination of benastatins a and b. | benastatins a and b, new inhibitors of glutathione s-transferase, have been isolated from the culture broth of streptomyces sp. mi384-df12. by x-ray crystallography, benastatin a was determined to be 8,13-dihydro-1,7,9,11-tetrahydroxy-13-dimethyl-8-oxo-3-pentyl- benzo[a]naphthacene-2-carboxylic acid. the structure of benastatin b was elucidated by nmr studies. | 1992 | 1429223 |
| reveromycins, new inhibitors of eukaryotic cell growth. iii. structures of reveromycins a, b, c and d. | reveromycins a, b, c and d are new group inhibitors of the mitogenic activity of epidermal growth factor (egf), produced by streptomyces sp. reveromycins are novel polyketide type antibiotics which have two terminal carboxylic groups, a spiroketal, a succinate and a varied side chain in the molecule. determination of their structures by chemical and spectroscopic methods, in particular nmr studies, is described. | 1992 | 1429227 |
| metacytofilin, a novel immunomodulator produced by metarhizium sp. ta2759. | | 1992 | 1429244 |
| preprotachykinin a mrna detected by in situ hybridization in guinea pig vestibular ganglion. | in situ hybridization histochemistry was conducted to examine the expression of mrna encoding preprotachykinin a (ppta), the precursor of the neuropeptide substance p (sp) and neurokinin a (nka), in guinea pig vestibular ganglion. signals for ppta mrna moderately accumulated on most ganglion cells. sp and nka are thus confirmed to be present in vestibular ganglion cells of the guinea pig. | 1992 | 1429248 |
| multiple forms of bile salt hydrolase from lactobacillus sp. strain 100-100. | four isozymes of bile salt hydrolase (bsh) have been purified from the cytosol of cells of lactobacillus sp. strain 100-100. the four proteins were designated bsh a, b, c, and d. they eluted from anion-exchange high-pressure liquid chromatography columns at 0.15, 0.18, 0.21, and 0.25 m nacl, respectively. they are catalytically similar, except that the vmax of bsh d is about 10-fold lower than those of the other three isozymes. all four proteins consist of one or two polypeptides. the peptides h ... | 1992 | 1429446 |
| studies on the uronic acid-containing glycoproteins of fusarium sp. m7-1: iv. isolation and identification of four novel oligosaccharide units derived from the acidic polysaccharide chain. | four novel oligosaccharide units were isolated from the acetolysis products of the acidic polysaccharide chain derived from the glycoproteins of fusarium sp. m7-1. their chemical structures were resolved mainly by 1h-nmr spectrometry in combination with methylation analysis and mass spectrometry. the results indicate that these oligosaccharide units originated from the side chains, glcnac alpha 1-->4glca alpha 1-->2(glcnac alpha 1-->4)glca alpha 1-->2gal, glcnac alpha 1-->4glca alpha 1-->2(glcna ... | 1992 | 1429523 |
| excitation energy transfer from phycocyanin to chlorophyll in an apca-defective mutant of synechocystis sp. pcc 6803. | a greenish mutant of the normally blue-green cyanobacterium synechocystis sp. pcc 6803, designated uv6p, has been isolated and characterized. uv6p possesses functional photosystems i and ii (psi and psii) but lacks normal light harvesting phycobilisomes because allophycocyanin is absent and core-specific linker proteins are almost entirely absent. the mutation responsible for the uv6p phenotype has been identified; it is a base substitution which results in the creation of a termination codon wi ... | 1992 | 1429645 |
| mycoplasma ovipneumoniae infection in zimbabwean goats and sheep. | the prevalence of mycoplasma ovipneumoniae (mo) in three communal lands flocks of goats on the outskirts of harare is described. in a single sampling of nasal swabs from 172 randomly selected, apparently healthy goats, 112 animals were identified as carriers of mo. the three flocks had a history of occasional deaths, following chronic and subacute pneumonia associated with isolation of mo as the only identifiable pathogen. attempts to induce clinical pneumonia in groups of 6 to 8-week-old kids a ... | 1992 | 1430348 |
| nature and distribution of mineral-binding, keratan sulfate-containing glycoconjugates in rat and rabbit bone. | the presence of keratan sulfate (ks) and ks proteoglycans in bone has been demonstrated in birds and rabbits but comparison with other animal species has not been investigated. the nature and distribution of mineral-binding, ks-containing glycoconjugates in rat and rabbit bone were investigated with a monoclonal antibody (mab 5d4) specific for ks. mineral-binding proteins were extracted from the mineralized bone with 0.4 m edta without guanidine-hcl (e-extract). on western blot analysis of sds-p ... | 1992 | 1431063 |
| isospora solimanae sp. nov. from the egyptian hoopoe (upupa epops major). | isospora solimanae (sp. nov.) is recorded for the first time infecting the egyptian hoopoe (upupa epops major). unsporulated as well as sporulated oocysts were studied, photographed and compared with other isosporan infections of related birds. site of infection and invasion limits were determined among naturally infected hoopoes and represented diagramatically. the rate of infection was congruent to 37.5%, and its severity considered to be non-pathogenic, since no remarkable lesions were observ ... | 1992 | 1431275 |
| new bioactive metabolites from a freshwater isolate of the fungus kirschsteiniothelia sp. | the new cytotoxic naphthoquinone dimer kirschsteinin [5], two new chlorinated diphenyl ethers 8 and 9, three known naphthoquinone derivatives 1, 2, and 7, a monoacetyl derivative of 2, and the (-)-enantiomer of o-methylasparvenone [4], have been isolated from a previously undescribed species of kirschsteiniothelia. the structures of these compounds were assigned primarily by nmr studies and by spectral comparisons. | 1992 | 1431934 |
| impact of zinc supplementation on intestinal permeability in bangladeshi children with acute diarrhoea and persistent diarrhoea syndrome. | zinc has been shown to enhance intestinal mucosal repair in patients suffering from acrodermatitis enteropathica; but the impact on mucosal integrity during acute (ad) or persistent (pd) diarrhoea is unknown. one hundred eleven children with ad and 190 with pd aged between 3 and 24 months received, randomly and blind to the investigators, either an elemental zinc supplement of 5 mg/kg body wt/day or placebo in multivitamin syrup for 2 weeks while intestinal permeability and, biochemical and anth ... | 1992 | 1432467 |
| effects of dietary oils on pulmonary vascular reactivity and chronic hypoxic pulmonary hypertension in rats. | the effects of dietary oils on pulmonary vasoreactivity and chronic hypoxic pulmonary hypertension were assessed in rats fed on soybean oil (so), pig oil (po), soybean oil plus pig oil (sp) or a regular diet (hc) started two months before the onset of chronic normobaric hypoxia (10 +/- 0.5% o2 ventilation, 8 h per day, for 14 days). the mean pulmonary arterial pressure and pulmonary vasoreactivity to acute hypoxia were higher in the so group than in the po, sp and hc groups. hypertrophy of the r ... | 1992 | 1433419 |
| magnetic resonance imaging of myocardial kinematics. technique to detect, localize, and quantify the strain rates of the active human myocardium. | a magnetic resonance imaging (mri) method is presented to detect, localize, and quantify myocardial kinematics by measuring the material rate-of-strain tensor at each pixel in gated nmr images of the heart. the immediate, local effect of muscular activity is self-deformation, and the strain tensor is the basic mathematical device by which such deformation may be quantified. the present method, called "strain-phase" mri (sp-mri), entails four steps: (1) the velocity of the myocardium is encoded b ... | 1992 | 1435210 |
| characterization of carbohydrate metabolism and demonstration of glycosomes in a phytomonas sp. isolated from euphorbia characias. | phytomonas sp. isolated from euphorbia characias was adapted to sdm-79 medium. cells isolated in the early stationary phase of growth were analyzed for their capacity to utilize plant carbohydrates for their energy requirements. the cellulose-degrading enzymes amylase, amylomaltase, invertase, carboxymethylcellulase, and the pectin-degrading enzymes polygalacturonase and oligo-d-galactosiduronate lyase were present in phytomonas sp. and were all, except for amylomaltase, excreted into the extern ... | 1992 | 1435859 |
| properties of a partially purified acid phosphatase from pathogenic nocardia brasiliensis. | like many other bacteria, nocardia sp. possess acid phosphatase activity. in n. brasiliensis, a human and animal pathogen, this activity was resolved into two enzyme forms by native gel electrophoresis. one (isozyme i) was partially purified and characterized. it exhibited an estimated molecular weight on sds-page of 50 kda, a ph optimum of 5.2, and a km value of 1.25 mm for p-nitrophenylphosphate. the n. brasiliensis enzyme was stable at 4 degrees c for at least 24 h, but readily inactivated at ... | 1992 | 1435947 |
| disseminated aspergillus terreus infection in a caged pigeon. | disseminated aspergillosis due to aspergillus terreus was diagnosed in a young pigeon kept by a bird fancier. the fungus was isolated in heavy growth from the infected tissues on sabouraud medium at 37 degrees c. microscopic examination of the squeeze preparation of the air sacs revealed the presence of typical conidiophores of an aspergillus sp. histologically, branched, septate hyphae morphologically indistinguishable from aspergillus were detected in the air sacs and lungs. the clinical, myco ... | 1992 | 1435955 |
| experimental infection of pigs with a taenia species from korea: parasitological and serological aspects. | belgian landrace piglets were experimentally infected with eggs of a taenia sp. of korean origin. at autopsy, metacestodes were present only in the livers. the proportion of degenerated metacestodes increased from 12%-39% at 5 weeks to 94%-100% at 10 weeks after infection. a sandwich enzyme-linked immunosorbent assay (elisa) using monoclonal antibodies raised against the excretory-secretory products of t. saginata metacestodes detected circulating antigen in the sera of the pigs at 1 week post-i ... | 1992 | 1438138 |
| sarcocystis jorrini sp. nov. from the fallow deer cervus dama. | sarcocystis jorrini sp. nov. isolated from the muscle of naturally infected fallow deer (cervus dama) from the doñana national park (spain) is described by light and electron microscopy. sarcocysts were macroscopic (2.7 x 1.4 mm) and were found in all striated muscle, including that of the esophagus and the heart. as visualized by light microscopy, the cyst walls were formed by mammilated protrusions surrounded by a coating of connective tissue measuring 10-35 mm in thickness. electron microscop ... | 1992 | 1438146 |
| immunocytochemical localization of a cysteine protease in adult worms of the liver fluke fasciola sp. | the intracellular localization of a cysteine protease from fasciola sp. that hydrolyzes host hemoglobin as a nutritional source was examined by light and electron microscopic immunocytochemistry using a monoclonal antibody specific to the enzyme. immunoperoxidase staining was predominantly restricted to large numbers of granules in the parasite intestinal epithelial cells and to host erythrocytes present in the intestinal lumens. in immunogold electron microscopy, the gold particles were consist ... | 1992 | 1438149 |
| selection of a thermostable variant of chloramphenicol acetyltransferase (cat-86). | the moderate thermophile bacillus stearothermophilus was used as a host in which to detect more thermostable variants of the b.pumilus chloramphenicol acetyltransferase (cat-86) protein. seventeen mutants were isolated and detected by their ability to grow in the presence of chloramphenicol at a previously restrictive temperature (58 degrees c). the genes encoding these proteins were sequenced; all 17 mutants carried the same c to t transition that conferred an amino acid substitution of alanine ... | 1992 | 1438164 |
| a structural role of histidine 15 in human glutathione transferase m1-1, an amino acid residue conserved in class mu enzymes. | his15 is a conserved amino acid residue in all known class mu glutathione transferases. this his residue in human glutathione transferase m1-1 has been mutated into 17 different amino acid residues by means of site-directed random mutagenesis to determine if any substitutions are compatible with catalytic activity. the majority of the mutant proteins appeared unstable and could not be isolated in reasonable quantities by heterologous expression in escherichia coli. five mutant enzymes, h15c, h15 ... | 1992 | 1438166 |
| microbial flora in gut of culex quinquefasciatus breeding in cess pits. | the number and types of microorganisms in the gut of culex quinquefasciatus larvae varied considerably from one site of collection to another. larval gut, in general, contained enormous number of bacteria, a few fungi and negligible number of actinomycetes which belonged to 15 bacterial, 6 fungal and 4 actinomycete genera, respectively. bacillus sp., staphylococcus sp. and pseudomonas sp. among bacteria, aspergillus among fungi and streptomyces sp. among actinomycetes were frequently encountered ... | 1992 | 1439987 |
| occurrence of the mycotoxin chlamydosporol in fusarium species. | the mycotoxin chlamydosporol (c11h14o5) has been independently isolated from three different fusarium species (f. chlamydosporum, f. acuminatum and f. culmorum), suggesting that its distribution may be widespread. we have developed a sensitive, specific fluorometric assay for chlamydosporol that allows quantitation in crude culture extracts. using this assay we have observed production of chlamydosporol on rice medium by 23 of 40 isolates (58%) of fusarium sp. examined in amounts as high as 0.8% ... | 1992 | 1440633 |
| igg antibodies to loxosceles sp. spider venom in human envenoming. | the presence and specificity of igg antibodies produced by patients with loxoscelism were studied. the loxoscelism diagnosis was supported mainly by clinical parameters. a search for igg antibodies anti-loxosceles gaucho venom in patients with loxoscelism submitted to serumtherapy showed antibodies in four out of 20 patients. the igg antibodies were detected as early as 9 days and as late as 120 days after bite. the highest igg antibody titer was 1:640 and the lowest was 1:80. immunoblotting tes ... | 1992 | 1440649 |