| molecular internalization of a region of myelin basic protein. | the conformation of myelin encephalitogenic or basic protein (bp) was investigated with a double-antibody radioimmunoassay by studying the reaction of bp or its fragments with antibodies produced in two rabbits against peptide 43-88 linked to rabbit serum albumin. both antisera reacted well with peptide 43-88 but showed little or no reaction with bp. absorption of these antisera with a bp-immunoadsorbent did not remove the antibody activity against peptide 43-88. within the region of peptide 43- ... | 1977 | 68996 |
| biological properties of human leukocyte interferon components. | human leukocyte interferon, purified approximately 1000-fold by affinity chromatography on immobilized anti-interferon globulins and sds-sephadex filtration, was resolved into one major and one minor component by adsorption chromatography on hydroxylapatite and electrophoresis in polyacrylamide gels. these components were indistinguishable in their capacity to protect bovine, porcine and murine cells, and the antiviral activities of both were equally susceptible to reduction by beta-mercaptoetha ... | 1977 | 69014 |
| staining reticulin in beef dorsal lymph nodes for electron microscopy. | reticulin has been classically identified by virtue of its argyrophilia, as observed by light microscopy. the similiarity between reticulin and collagen has been a matter for considerable discussion. this paper describes the application of a silver-staining procedure which demonstrates the fine structure of these fibres when studied by electron microscopy, giving a direct indication of the morphological identity of the argyrophilic structures, i.e. reticulin, with collagen. beef lymph node tissu ... | 1977 | 69030 |
| detection of infantile gastroenteritis virus (rotavirus) by elisa. | | 1977 | 69078 |
| polybrominated biphenyls, polychlorinated biphenyls, pentachlorophenyl--and all that. | | 1977 | 69104 |
| hydatid disease in bangladesh. | | 1977 | 69114 |
| ox eyes and immunosurveillance. | | 1977 | 69201 |
| the effects of proteins upon the filtration coefficient of individually perfused frog mesenteric capillaries. | | 1977 | 69248 |
| comparison of two dry cow intramammary treatments. | | 1977 | 69358 |
| [modification of the immune reaction by antigen-immunosuppressive-agent conjugates. ii. immunogenicity of antigen-immunosuppressive-agent conjugates]. | the maintenance of specific immunogenicity of carrier proteins is a necessary condition for the successful use of antigen-immunosuppressive agent-conjugates (aic) for an antigen specific suppression of the immune response. the experimental results indicate that, in spite of the binding of 6-mercaptopurine (6-mp) and toluyl (t) to bovine gamma globulin (bgg) and human serum albumin (hsa), the carrier specific immunogenicity is not significantly altered. the intradermal application of 6-mp-bgg and ... | 1976 | 69373 |
| [modification of the immune reaction by antigen-immunosuppressive agent-conjugates. iii. physiocochemical, antigenic and functional properties of 6-mercaptopurine coupled carrier proteins]. | 6-mercaptopurine (mp) conjugates of bovine gamma globulin (bgg) and human serum albumin (hsa) with increasing numbers of mp-groups per protein molecule were prepared and some physicochemical and antigenic properties studied. the electrophoretic mobility increased proportionally to the degree of substitution. in the same manner the formation of high molecular weight aggregates increased. a loss of bgg and hsa specific determinants was observed too. the physicochemical and antigenic properties of ... | 1976 | 69374 |
| [modification of the immune reaction by antigen-immunosuppressive-agent conjugates. iv. studies on the specific suppression of humoral immune response in guinea pigs by antigen-immunosuppressive-agent conjugates]. | bovine gamma globulin (bgg) antigens were modified by the binding of 6-mercaptopurine and toluyl residues, and their influence on the humoral immune response in guinea pigs was investigated. the antigen-immunosuppressive agent-conjugates (aic) were different, depending on the method used for their preparation and the number of coupled residues per one molecule of bgg. conjugates denoted as mpi-n-bgg were prepared by special chemical binding of corresponding thioisocyanates. mpii-n-bgg were synth ... | 1976 | 69375 |
| [studies on the significance of antigen in the 6-mercapopurine-influenced humoral antibody response in guinea pigs]. | the mechanism of suppression of humoral immune response to dinitrophenylated bovine gamma globulin (dnp23-bgg), human serum albumin (hsa), and trinitrophenylated sheep red blood cells (tnp-srbc) by 6-mercaptopurine (6-mp) was studied in guinea pigs. following the intradermal application of the antigens emulsified in complete (cfa) or incomplete freund's adjuvant (ifa) each test animal was given 6-mp, 10 mg/kg/day for 7 days. this treatment resulted in a significant suppression of the anti bgg an ... | 1976 | 69376 |
| cell-mediated immune response to guinea pig and bovine basic proteins of myelin in lewis and pvg rats and their hybrids. | the in vitro cell-mediated immune response of rat lymph node cells (lnc) to guinea pig and bovine encephalitogenic protein (ep) has been studied with a lnc transformation test. lnc were obtained from either lewis, pvg, or f1 (lewis x pvg) rats 28 days after challenge with guinea pig or bovine ep in freund's complete adjuvant (fca) of different mycobacterium content. no differences between the strains or their hybrids in lnc response could be shown if stimulated with sensitizing ep, despite the g ... | 1977 | 69388 |
| [the influence of 6-mercaptopurine on the humoral immune response in guinea pig. 2. the influence on formation of complement binding antibodies (author's transl)]. | the effectivity of treatment with 10 mg 6-mercaptopurine/kg/day--beginning on the day of antigen injection and continuing for seven days--on the synthesis of complement binding antibodies is very different. the formation of gamma-2-antibodies against bgg was nearly complete suppressed, an influence on the anti-dnp-gamma-2-antibody formation was not detectable and the antibody formation against hsa was enhanced. the results suggest that the kind of antigen is very important for the immunosuppress ... | 1977 | 69392 |
| [conjugation of bovine gammaglobulin to l-asparaginase (e. coli) (author's transl)]. | bovine gammaglobulin-l-asparaginase conjugates were prepared with an enzymic activity of about 30 iu/mg. glutardialdehyde was used as bifunctional reagent for the cross-linking in an optimal concentration of 0.16 mg/10 mg protein. the both components were found in the conjugates in a ratio of 1:1.5. the recovery was about 25% of the initial protein amounts. the antigenic specificity of the globulin component decreased up to 60%, the enzymic activity also up to 60% by the conjugation. | 1977 | 69393 |
| [effect of l-asparaginase on humoral immune response against protein antigens in mice (author's transl)]. | the immune response of cba-mice against alum adsorbed bgg is depressed by daily injections of 20, 40 and 80 i.u. of asparaginase (crasnitin) from the first day before to the second day after immunisation. after a period of 14 or 21 days the antibody levels slowly rises again. the early igm-response is not altered. pretreatment of the animals with bgg-asparaginase conjugate has not any influence on the immune reaction. the conjugate is because its high molecular weight more immunogenic than the b ... | 1977 | 69394 |
| [the influence of 6-mercaptopurine on the humoral immune response in guinea pig. 1. the importance of antigen and adjuvans (author's transl)]. | following an intradermal immunization of guinea pigs with dinitrophenylated bovine gamma globulin (dnp23-bgg), human serum albumin (hsa) and trinitrophenylated sheep red blood cells (tnp-srbc) emulsified in complete or in incomplete freund's adjuvans the effectivity of 6-mercaptopurine (6-mp) on the formation of specific antibodies was investigated. by application of 10 mg 6-mp/kf/day--in guinea pigs a significant depression of antibody formation against bgg could be demonstrated if treatment is ... | 1977 | 69395 |
| [the macrophage disappearance reaction in guinea pigs sensitized with bovine gamma globulin or human scrum albumin (author's transl)]. | the macrophage disappearance reaction (mdr) is a suitable test for detection of cell mediated immunity against bovine gamma globulin (bgg) and human serum albumin (hsa) in guinea pigs. the mdr is a technical simple, good manipulable, and quantifiable test. the optimal test conditions for the antigens bgc and hsa are the following: peritoneal exudat cells (pec) were stimulated with paraffin oil. on the 5th day after receiving oil the animals were injected with 80 microgram bgg or 30 microgram hsa ... | 1977 | 69396 |
| evidence suggesting the presence of common antigenic determinant between dynein and tubulin. | the present experiments showed that the guinea pig antiserum prepared against the main polypeptides of 14 s dynein from tetrahymena cilia reacted with sea urchin sperm flagellar dynein and with bovine brain high molecular weight protein to give rise to a precipitin line confluent with that formed between the antiserum and tetrahymena dynein. furthermore, it was found that this antiserum also reacted with tubulins from tetrahymena cilia, sea urchin sperm flagella and bovine brain to give rise to ... | 1977 | 69447 |
| a methodology for the production of carbohydrate-specific antibody. | 8-methoxycarbonyloctyl glycosides are recommended as precursors for the preparation of artificial antigens that possess strongly immunodominant carbohydrate antigenic determinants. the preparation and immunochemical properties of a selected number of these antigens are reported to demonstrate the high levels of hapten-specific antibodies that are normally achieved using standard immunization schedules. the method, based on glycoside synthesis, promises to make available a wide spectrum of well d ... | 1977 | 69478 |
| kinetics of the inactivation of human and bovine trypsins and chymotrypsins by alpha1-proteinase inhibitor and of their reactivation by alpha2-macroglobulin. | the time dependency of inactivation of human cationic trypsin and chymotrypsin ii and of bovine trypsin and alpha-chymotrypsin by human serum has been investigated. since the molar concentration of serum alpha1-proteinase inhibitor is much higher than that of other inhibitors, this time dependence could be used to calculate the rate constants kass for the association of alpha1-proteinase inhibitor with the four proteases. the association process was found to be second order, with kass ranging fr ... | 1977 | 69511 |
| antigen receptors of t helper cells. | | 1977 | 69518 |
| hyperacute allergic encephalomyelitis: a single determinant. | heae cannot be induced in either guinea pigs using 10(10) or 20x10(10) organisms of b. pertussis or in rabbits using 4x10(10) organisms of b. pertussis and basic protein from the following species: guinea pig, lewis rat, human, bovine, porcine, monkey and rabbit. the only encephalitogenic region for lewis rats which also produces heae in lewis rats is not encephalitogenic in either rabbits or guinea pigs. therefore it seems highly probably that there is only one heae determinant for all species ... | 1977 | 69608 |
| specificity of the glial fibrillary acidic protein for astroglia. | glial fibrillary acidic protein (gfa) is the main constituent of glial filaments and the close similarity of gfa and neurofilament protein has been recently reported. however, the immunofluorescence staining of peripheral nerve which may be observed with gfa antisera is not due to cross-reaction between gfa and neurofilament protein. staining of peripheral axons was also observed with control sera obtained by injecting the rabbits with nonimmunogenic gfa preparations isolated with the same proce ... | 1977 | 69656 |
| distribution and optical activity of the basic protein in bovine peripheral nerve myelin. | | 1977 | 69680 |
| improvement of fertility in post-partum beef cows by treatment with pmsg and progestagen. | | 1977 | 69709 |
| effect of concanavalin a on ocular immune responses. | | 1977 | 69710 |
| squirrel monkey retrovirus: an endogenous virus of a new world primate. | squirrel monkey retrovirus (smrv) was isolated by cocultivation of squirrel monkey lung cells with canine cells. 3h-labeled 60-70s smrv rna, isolated from virus grown in canine cells, hybridized to the same extent and to the same cot1/2 value to the dna of all tissues of all squirrel monkeys tested; cot1/2 values show that smrv proviral sequences are present in the low repetitive range. no smrv proviral sequences were detected in tissues from a variety of other new world monkeys, old world monke ... | 1977 | 69724 |
| characterization of a retravirus isolated from squirrel monkeys. | a new retravirus (smrv) isolated from a squirrel monkey, saimiri sciureus, has an mg2+-dependen reverse transcriptase and a buoyant density of 1.17 g/cm3 in sucrose and 1.21 g/cm3 in cesium chloride, similar to the mouse mammary tumor virus and the mason-pfizer monkey virus. the polypeptide patter of smrv as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis was distinct from the reported polypeptide patterns of known retraviruses. four major polypeptides of molecular weight ... | 1977 | 69728 |
| biochemical and immunological characterization of the major envelope glycoprotein of bovine leukemia virus. | the major envelope glycoprotein of bovine leukemia virus was isolated by lectin-bound sepharose and deae-cellulose column chromatography. this protein was shown to have a molecular weight of about 41,000 and to lack detectable immunological cross-reactivity with glycoproteins of other oncornaviruses. sera obtained from 100% of cattle examined with clinically diagnosed lymphosarcoma contained high-titered antibody to 125i-labeled bovine leukemia virus glycoprotein, whereas sera from animals in a ... | 1977 | 69730 |
| enzyme-linked immunosorbent assay (elisa) for detection of human reovirus-like agent of infantile gastroenteritis. | an enzyme-linked immunosorbent assay (elisa) was developed for the detection of the human reovirus-like agent of infantile gastroenteritis in human stools. the results of the assay can be read either with a simple colorimeter or the naked eye. investigations with 143 samples from children with gastroenteritis and 75 samples from children with other illnesses showed that the elisa was as sensitive as electron microscopy or radioimmunoassay for detection of this agent. in addition, the elisa was s ... | 1977 | 69877 |
| a new factor that may control collagen resorption. | specific collagenases responsible for the initial enzymic step leading to degradation of the collagen fibrils of connective tissues have been found in both latent and active forms. the most important factor controlling the local activity of collagenase extracellularly may be an inhibitor that is synthesised by connective tissues, and it is proposed that latent enzymes are all enzyme-inhibitor complexes. | 1977 | 69939 |
| ox eyes and immunosurveillance. | | 1977 | 69963 |
| residues of antibiotics in milk after intramammary application on high grade cows in kenya (zebu x frisian and zebu x ayrshire). | | 1977 | 70047 |
| inhibition of plasminogen binding to fibrin by alpha2-plasmin inhibitor. | | 1977 | 70081 |
| [identification of the chromosomes in the karyotype of cows (bos taurus) by differential gimsa staining with subsequent densitometry]. | | 1977 | 70092 |
| [antigenic structure of the plasma membranes of mouse liver cells. iii. lipid haptens]. | the total glycolipids, gangliosides, neutral lipids and some individual lipids of these fractions were isolated from the whole lipid extract of the mouse liver cell suspension. the antigenic activity of the isolated lipids was studied with the complement fixation test using the rabbit antisera against the liver cell ghosts. active lipids were: glycosphingolipids, gangliosides, cardiolipin and fraction containing polar glyco- and phospholipid mixture. the cardiolipins isolated from animal cells o ... | 1977 | 70096 |
| [synthesis, physico-chemical and antigenic properties of the conjugated antigens of xanthine- and theophylline proteins]. | conjugated antigens of xanthine- and theophylline-protein were synthesized by the reaction of diazosalts of 8-aminoxanthine and 8-aminotheophylline with bovine serum albumin. uv-spectra of the synthesized preparations were studied and content of xanthine and theophylline, covalently bound with the substances, was estimated. immunization of animals with the conjugates was found to cause the occurrence of antibodies in blood serum, which bound specifically various derivatives of purine. | 1977 | 70110 |
| purification and physicochemical properties of antithrombin. (heparin cofacter) (author's transl)]. | | 1977 | 70145 |
| cell culture studies with a cytopathic bovine rotavirus. | the growth characteristics in mdbk cells of a calf rotavirus isolated in northern ireland are described. of a range of cell cultures tested, the virus grew in secondary bovine kidney and mdbk cells, but consistently produced a cpe only in mdbk cells. the cpe consisted of cytoplasmic vacuolation, development of eosinophilic intracytoplasmic inclusions, and degeneration and detachment of cells from the monolayer. the onset of cpe was more rapid and its effects more severe in rolled cultures than s ... | 1977 | 70198 |
| bovine homologue of beta2-microglobulin isolated from milk. | | 1977 | 70205 |
| [conduct of bacillus subtilis transformation in mice under conditions of immunologic suppression of exogenous dnaase 1 activity]. | bacillus subtilis transformation was conducted in the abdominal cavity of mice. the frequency of transformation was considerable decreased when bovine dna-ase 1 (3-- 5microgram) was injected intraperitoneally to these animals. immune rabbit gamma-globulins containing antibodies to bovine dna-ase 1 inhibited in vivo the activity of dna-ase 1, protected transforming dna from the hydrolyzing effect of this enzyme. this model can be utilized in search for ways to preserve dna injected into the anima ... | 1977 | 70246 |
| role of gangliosides in the uptake and retrograde axonal transport of cholera and tetanus toxin as compared to nerve growth factor and wheat germ agglutinin. | previous investigations have shown that tetanus toxin is transported retrogradely in all peripheral neurons whereas the transport of ngf is confined to adrenergic and sensory neurons. other macromolecules with molecular weights and general physiochemical properties similar to ngf and tetanus toxin (e.g., cytochrome c, insulin, horseradish peroxidase and bovine serum albumin) are not transported to a detectable extent if injected in comparable molar concentrations. for tetanus toxin, which is tra ... | 1977 | 70259 |
| effects of albumin and other proteins during assay of amylase activity. | addition of albumin, gamma-globulin, alpha-casein or submaxillary mucin to the assay system for chromogenic measurement of human or rat amylase with blue starch increased the amylase activity, albumin having the most effect. these proteins seemed to increase the activity by protecting amylase from inactivation. amylase activity was higher in urine samples showing proteinuria than in urine samples without detectable protein. it is concluded that amylase assay is more reliable when a final concent ... | 1977 | 70283 |
| metabolic instability of myelin proteins. breakdown of basic protein induced by brain cathepsin d. | | 1975 | 70349 |
| adjuvanticity (immunity-inducing property) of cord factor in mice and rats. | cord factor, a glycolipid in mycobacteria, was found to make a tolerogenic protein antigen immunogenic when injected 0 to 2 days before tolerogen injection in mice and rats. cord factor was also found to increase phagocytic function of the reticuloendothelial system of mice, and the relationship between the immunity-inducing capacity and the phagocytic function is discussed. | 1977 | 70404 |
| measurement of mammalian sperm deoxyribonucleic acid by flow cytometry. problems and approaches. | measurement of mammalian sperm deoxyribonucleic acid content is of importance in several areas of biomedical research. when measured in flow systems with orthogonal axes of illumination, flow and detection, an unexpected, distorted distribution consisting of a narrow peak with a lateral extension to the right is observed. several lines of evidence lead to the conclusion that this effect is an optical-geometric artifact attributable to the flat shape and high index of refraction of mammalian sper ... | 1977 | 70455 |
| immunosuppressive properties of a peptic fragment of bsa. | the immunogenic properties of a peptic fragment of bsa were investigated. bsa was subjected to limited proteolysis by pepsin and the resulting fragments were separated on deae cellulose. the fragment under consideration, fraction ia (m.w. 8000 to 10,000), did not precipitate with anti-bsa serum but did inhibi, the binding of specific antibody to labeled bsa, indicating the presence of determinants found on the native antigen. bdf1 mice immunized with fraction ia in a1 (oh)3 gel or in complete fr ... | 1977 | 70485 |
| preferential induction of jones-mote hypersensitivity in guinea pigs treated with tolerogenic antigen. | | 1977 | 70486 |
| genetic control of immune response to protein antigens. i. two-way selective breeding of mice for quantitative antibody responsiveness to bovine serum albumin and rabbit gamma-globulin. | | 1977 | 70487 |
| purification and characterization of precipitating antigens from theileria parva. | precipitating antigens from theileria parva have been partially purified. two antigens from each of the schizont and piroplasm stages of the parasite were identified; the major antigens from the two stages shared the same specificity. the antigens showed considerable molecular heterogeneity, almost certainly a result of the preparative method, and they always contained large amounts of dna. the piroplasm antigens were of parasite nuclear origin and the schizont antigens were probably of the same ... | 1977 | 70504 |
| [comparative study of the activity of cerebral thromboplastins of various vertebrates]. | | 1977 | 70565 |
| dna complementary to parathyroid mrna directs synthesis of pre-proparathyroid hormone in a linked transcription-translation system. | dna complementary in sequence to the messenger rna for pre-proparathyroid hormone was synthesised using reverse transcriptase. in a linked transcription-translation system using rna polymerase and cell-free extract from wheat germ, the dna directed the synthesis of a protein identified as pre-proparathyroid hormone by n-terminal sequencing and by electrophoretic and immunologic criteria. | 1977 | 70756 |
| ultrastructural immunoperoxidase cytochemistry. | | 1977 | 70829 |
| [neurospecific proteins and their possible physiologic functions]. | | 1977 | 70894 |
| [on the similarity of antigen between torulopsis and salmonella (author's transl)]. | the immune sera for torulopsis apicola, t. bovine, t. colliculosa, t. fructus, t. glabrata, t. haemulonii and t. kestoni agglutinated salmonella cholerae-suis (6,7). the immune serum for s. cholerae-suis agglutinated torulopsis apicola, t. bovina, t. colliculosa, t. fructus, t. glabrata, t. haemulonii and t. kestoni. absorption and agglutination cross test demonstrated common antigen factors in the tested torulopsis species and in s. cholerae-suis (6,7). | 1977 | 70908 |
| characterization of extract of dog hair and dandruff from six different dog breeds by quantitative immunoelectrophoresis. identification of allergens by crossed radioimmunoelectrophoresis (crie). | an extract of mixed dog hair and dandruff from six different dog breeds (alsatian, boxer, collie, poodle, and long-haired and short-haired dachshund) was obtained by mild extraction, centrifugation, dialysis and freeze-drying. extract of hair and dandruff from the individual dog breeds was obtained in the same way, but the material was not freeze-dried. examination and characterization of the mixed extract by means of crossed immunoelectrophoresis revealed a precipitation pattern composed of 25 ... | 1977 | 70943 |
| combined staining of corneal endothelium by alizarine red and trypane blue. | combined staining by alizarine red and trypane blue is an easy and reliable method for in vitro visualization of corneal endothelial nuclei, cellular borders and uncovered parts of the membrane of descemet. a stained full thickness wet mount can be prepared in less than five min. the staining method and preliminary findings concerning endothelial morphology in the centre and in the periphery of normal corneas are presented. | 1977 | 70953 |
| the transfer of bacterial biotin genes to escherichia coli k-12 cells by transfection with lambda bio phage dna. | the transfection of biotin genes to biotin negative escherichia coli k-12 cells with donor dna isolated from transducing lambda bio phages was examined. the transfection of the cells was performed in the presence of bovine serum albumin and sucrose (osowiecki and skalińska, 1974). | 1977 | 70968 |
| fimbriae and haemagglutinating properties in edwardsiella and levinea. | using the haemagglutinating procedure, the presence of fimbriae was revealed in all 34 edwardsiella strains tested and in 3 levinea strains out of 17 examined. the great majority of fimbriate edwardsiella strains caused haemagglutination of mr type; only two strains of edwardsiella and all fimbriate strains of levinea brought about haemagglutination of ms type. all the strains exhibited a similar spectrum of haemagglutinating activity when they were tested against red cells of various animal spe ... | 1977 | 70969 |
| residue content of beef feedlot manure after feeding diethylstilbestrol, chlortetracycline and ronnel and the use of stirofos to reduce population of fly larvae in feedlot manure. | two beef cattle feedlot experiments were conducted to determine the amount of certain agricultural chemicals that are likely to be found in both fresh and stored feedlot manure and to investigate fly control in this manure. in experiment 1, diethylstilbestrol (des), chlortetracycline (ctc), and ronnel were used as feed additives. fresh manure, stored manure, runoff water, manure weathered on pasture, and soil from pasture fertilized with manure were analyzed for these additives. stirofos was add ... | 1977 | 71021 |
| [study of dna extracted from the organs of animals artificially contaminated by tritium in an organic form]. | | 1976 | 71050 |
| electrophoretic separation of thyroid rna. | | 1976 | 71056 |
| [studies on the virus demonstration in enzootic bovine leukosis using the revertase test]. | this exogenic test for the detection of enzyme activities (revertase) is helpful in differentiating between leucocytes of leukaemic cattle, on the one hand, and those of intact animals, on the other. the mechanism of such identification is based on differences in enzyme activity in the presence of poly ra/oligo dt and poly da/oligo dt. the best results were obtained from short-time cultured leucocytes which gave ten times higher margins of incorporation by comparison between intact and preleucot ... | 1977 | 71129 |
| [studies on the excretion of estrogen in cows following oral and percutaneous (pour-on) administration of chlormadinone acetate]. | the oestrogen levels were assessed in 180 urine samples taken from 20 cows. the animals had received over 15 days 40 mg one-per-cent oily solution of chloromadinone acetate. the solution had been applied orally or by pour-on methods. the curves of oestrogen excretion were identical for both methods of application, both during and after completed administration. in other words, the effect obtainable from pour-on application is just as good as that obtainable from oral administration. | 1977 | 71130 |
| [critical electrolytic concentration for sodium acridine chloride orange in the differential histochemical characterization of sialomucins: a new indirect method]. | | 1975 | 71132 |
| high doses of subconjunctival corticosteroid and antibody-forming cells in the eye and draining lymph nodes. | after the intracorneal injection of rabbit eyes with bovine gamma-globulin, the subconjunctival administration of a corticosteroid in either the ipsilateral or contralateral eye resulted in essentially the same changes: a reduction in the number of antibody-forming cells in the draining lymph nodes and a reduction in the ocular inflammatory response. | 1977 | 71139 |
| measurement of fast light-induced disc shrinkage within bovine rod outer segments by means of a light-scattering transient. | | 1977 | 71161 |
| [genetic control of the content and avidity of antigen-binding b-lymphocytes in the mouse spleen]. | the content (per 10 nucleated cells) and avidity curves of igm positive b-lymphocytes forming rosettes with trinitrophenyl-sheep erythrocytes (tnp-rfc) were compared in two mouse lines (balb/c c57bl/6), f1 hybrids (balb /c x c57bl/6) and backcross hybrids (f1 x balb/c, f1 x c57bl/6). trinitrophenyl-bovine serum albumin (tnp24bsa) and dinitrophenyl-bonvine serum albumin (dnp23bsa) and sulfanil-bsa (sulf17-bsa) in different dilutions were used as inhibitors. balb/c spleen contained by 60% more tnp ... | 1977 | 71174 |
| hypocalcemic effect of aminoglycoside antibiotics in the dairy cow. | the effect of the parenteral administration of aminoglycoside antibiotics on the blood calcium concentration in dairy cows was investigated. gentamicin was tested in vitro in blood drawn from cows, dihydrostreptomycin was tested in nonlactating cows and neomycin was tested in postpartum cows. the total and bound calcium fractions were significantly reduced by all three antibiotics. no change occurred in the unbound calcium fractions. caution is advised in the use of these drugs in postpartum cow ... | 1977 | 71189 |
| a quantitative study of encephalitogenic protein and peptides in guinea pigs. | the encephalitogenic activity of bovine encephalitogenic protein (ep) at at doses from 1 to 33.5 microgram was studied in guinea-pigs. anencephalitogenicity can be demonstrated at a dose of 1 microgram of ep when given with freund's complete adjuvant. an increase in the dose of ep results in an increase in encephalitogenicity. data from a previous paper (lindh and bergstrand 1975) supplemented with new data on the encephalitogenic capacity of peptide 89--169 were compared concerning the incidenc ... | 1977 | 71246 |
| [several criteria of evaluation of foot-and-mouth disease virus reproduced in cell cultures in suspension]. | the study of the plaques produced by viruses asia and o1 reveals different properties which may depend on the virus or on the cell or its method of culture. antigenic differences and subsequent immunological differences correspond to differences in plaques. on the other hand, the cell line in suspension evolves both in its morphology and its receptivity during the passages. all these variations imply the observations of quantitative and qualitative criteria of evaluation in order to prepare vacc ... | 1976 | 71255 |
| restriction of the anti-bovine serum albumin response in rabbits immunized with micrococcus lysodeikticus. | rabbits capable of producing antibodies of restricted heterogeneity in response to micrococcus lysodeikticus are equally capable of producing antibodies of restricted heterogeneity to bovine serum albumin. these antibodies are produced when animals are simultaneously injected with micrococcus and bsa and their specificity is restricted to a small number of epitopes. these results suggest that micrococcal vaccines can induce the restriction of heterogeneity in antibodies raised against totally un ... | 1977 | 71263 |
| separation of the moloney leukemia virus-determined cell surface antigen (mcsa) from known virion proteins associated with the cell membrane. | cell membranes of moloney lymphoma cells (yac, of strain a origin) were solubilized by np40. the antigenicity of the solubilized protein fraction was assayed by inhibition of the corresponding cytotoxic reaction against yac target cells. the moloney leukemia virus (mlv)-determined cell surface antigen (mcsa) was detected with mouse antisera, produced by the repeated inoculation of heavily irradiated yac cells into syngeneic mice. virion proteins gp71, p30, p15, p12 and p10 were identified with g ... | 1977 | 71277 |
| the utility of antisera to canine growth hormone and canine prolactin for immunocytochemical staining of the dog pituitary gland. | using the immunoperoxidase technique and specific homologous antisera to highly purified canine prolactin (prl) and canine growth hormone (gh), selective immunocytochemical staining was localized in two different cell types in the pars distalis of the dog pituitary gland. the specificity and validity of both antisera were demonstrated by elimination of their immunostaining capacify after prior absorption with their respective antigens, while absorption with other antigens failed to decrease stai ... | 1977 | 71286 |
| a simple spot technique for thin layer immunoassays (tia) on plastic surfaces. | a simple and sensitive technique for visualization of antigen--antibody reactions is described. the property of many antigens to become adsorbed firmly on to a hydrophobic polystyrene surface while retaining their serological reactivity is taken advantage of. on a surface with adsorbed antigen the corresponding immune serum is applied spot-wise. the antigen--antibody reaction areas on the surface are characterized by a distinct hydrophilic condensation pattern when exposed to water vapour. the r ... | 1977 | 71329 |
| interaction between soluble immune complexes and glass-fiber filters. | the two components of soluble antigen--antibody complexes, at the antigen excess, exhibit an increase in their binding ability to glass-fiber filters. this is demonstrated in two systems. in the 125i-bsa--anti-bsa system the proportion of 125i-bsa bound to the filter is markedly increased in the presence of anti-bsa antibodies. more than 80% of the antibody bound bsa can be removed by passage through the filter. in the other system, mouse gamma globulin (mgg)--125i-anti-mgg, the proportion of an ... | 1977 | 71331 |
| ultrastructural effects of perfomic acid oxidation of epoxy-embedded tissue with and without early uranyl staining. | epoxy-embedded blocks of glutaradehyde and oso4 fixed adenohypophyseal tissue were immersed in performic acid, rinsed and sectioned for electron microscopy. sections exhibited a general image intensity equivalent to control sections but their stainability with both uranyl acetate and lead citrate stains was increased. it was concluded that osmium was not removed by performic acid oxidation. membranes of endoplasmic reticulum and the golgi apparatus were not visible, and other components (acidoph ... | 1977 | 71350 |
| haemagglutinin from rabies viruses. | | 1977 | 71417 |
| corynebacterium bovis as a cause of human disease. | corynebacterium bovis is a gram-positive rod which can cause bovine mastitis and rancidity in cream. on only one occasion has this organism been reported to cause human disease. six further cases are described in which infections were caused by c. bovis. in two patients the nervous system was involved; two patients had bacterial endocarditis; one had chronic otitis media; and one had a persistent leg ulcer. five patients recovered completely from the infection; the other died from endocarditis. | 1977 | 71496 |
| elisa for rotavirus. | | 1977 | 71624 |
| crystallographic studies of bovine beta2-microglobulin. | crystals of the bovine milk protein lactollin yield x-ray diffraction data extending to a resolution of 2.8 a. lactollin is a bovine analogue of beta2-microglobulin, a protein that is homologous in amino acid sequence to the constant domains of immunoglobulins and is the light chain of the human and murine major histocompatability antigens. the protein crystallizes in the orthorhombic space group p2(1)2(1)2(1) with a = 77.4, b = 47.9, and c = 34.3 a. the unit cell parameters and physical chemica ... | 1977 | 71731 |
| [comparative electrophoretic study of the molecular forms of alkaline phosphatase in the leukocytes of agricultural animals]. | the alkaline phosphatase enzyme, isolated by morton's method from leukocytes of sheep, goats, and pigs gave after agarose elctrophoresis two isoenzyme fractions moving to the positive pole at the sites of the alpha 1- and alpha 2-globulins of the blood serum. in bovine leukocytes, besides these two fractions there was a third one that moved more slowly in the zone of the beta-globulins. in horses the alkaline phosphatase of leukocytes produced a wide band within the zones of the beta-globulins a ... | 1977 | 71781 |
| [demonstration of the infectious rhinotracheitis-pustular vuluovaginitis virus (ibr-ipv) in the placenta and aborted fetuses of cows]. | a well expressed correlation was found to exist between the immunofluorescence and the histologic methods in the demonstration of ibr-ipv infection of fetuses and placentae in 75 cases of spontaneous undiagnosed abortions. if those that were positive by the immunofluorescence method were 100 per cent as many as 87 per cent of them showed coincidence with the results obtained by the histologic method. the isolation of the virus of ibr-ipv coincided in 44 per cent of the cases only. clearest speci ... | 1977 | 71782 |
| [experimental inoculation of calves with influenza virus a/csf/udmurtiia/116/73]. | | 1977 | 71786 |
| [modification of immune reactions by antigen-immunosuppressive agent conjugates. v. studies on antigen-specific activity of 6-mercaptopurine bovine gamma globulin conjugates on the humoral immune response in rabbits]. | rabbits treated daily and for seven consecutive days with 6-mercaptopurine bovine gamma globulin conjugates (mpi--n-bgg; 'i'--characterizes the king of chemical binding and 'n' the number of coupled mp-residues per one mole bgg) show an altered immunological reactivity. a following intradermal immunization with bgg alum adsorbate results in a suppressed anti bgg antibody production on the third day after antigen application (antibody titer 1:320, antibody titer of control animals--pretreated wit ... | 1977 | 71798 |
| agarose gel electrophoretic fractionation of serum proteins in adult cattle. ii. a study of cows with different diseases. | | 1977 | 71851 |
| anhydrous hydrogen fluoride deglycosylates glycoproteins. | | 1977 | 71863 |
| [inhibition of dnase i activity in vivo]. | immune gamma-globulins containing antibodies to bovine dnase i inhibit activities of bovine and mouse dnase i both in vitro and in vivo. bovine dnase i was used as exogenous dnase i in the in vivo studies and was injected to mice intraperitoneally in combination with gamma-globulins. the serous fluid of mice was used as a source of endogenous dnase i. both in in vitro and in vivo studies immune gamma-globulins caused a practically complete inhibition of bovine dnase i activity, whereas the activ ... | 1977 | 71924 |
| [induction of transplantable leukemia-like syndromes in mice by administration of antigens and immunostimulants]. | splenomegaly characterised by myeloid metaplasia was induced in balb/c mice by the injection of antigens and immunostimulants. in difference from leukemoid reaction, this syndrome was transmissible both by the plasma and by the splenic cells. small virus-like particles (30--50 nm) were revealed in the plasma, and rna-containing viruses of type c--in the splenic cells. | 1977 | 71928 |
| reverse transcription of thyroglobulin 33-s mrna. | bovine thyroglobulin 33-s mrna has been used as a template for the synthesis of a complementary dna, using rna-directed dna polymerase from the avian myeloblastosis virus. the yield of the reaction was relatively poor and the size of the cdna did not exceed 10 s. nevertheless, a copy of high specific radioactivity (approximately 10(7) counts. min-1 microgram-1) could be obtained which hybridized specifically back to its template with an rot1/2 value about 5 times higher than that observed in hyb ... | 1977 | 71991 |
| comparison between purified alpha2-macroglobulin preparations from normal controls and patients with cystic fibrosis. kinetic and structural properties of the complex with bovine trypsin. | | 1977 | 72070 |
| vitamin d binding factors in bovine blood. | both 25 [25,26-hydrogen-3] hydroxycholecalciferol and [1alpha,2alpha-hydrogen-3] cholecalciferol were added to bovine plasma in vitro. analysis by gel-filtration and ion exchange chromatography, electrophoresis, ultracentrifugation, competitive binding specificity studies, and plasma stripping showed that vitamin d circulated with a protein of alpha-globulin mobility. this globulin had a much higher affinity for 25-hydroxycholecalciferol while vitamin d3 appeared to be associated first with an a ... | 1977 | 72082 |
| determination of residues of chlorpyrifos, its oxygen analogue, and 3,5,6-trichloro-2-pyridinol in tissues of cattle fed chlorpyrifos. | | 1977 | 72084 |
| quantitation of cellular deoxyribonucleic acid by flow microfluorometry. | this report characterizes for the first time an easy, reproducible means of standardizing the relative fluorescent units normally reported for flow microfluorometry. absolute values for deoxyribonucleic acid/cell are obtained by using nucleated red blood cells as references. cell were selected and characterized for the quantitative analysis of deoxyribonucleic acid per cell over a range from 2 pg/cell to 93 pg/cell using literature values for species having nucleated erythrocytes. fluorescence s ... | 1977 | 72097 |
| histologic and biochemical identification and characterization of an elastin in cartilage. | histochemical and chemical techniques have been used to identify, isolate and characterize elastin from certain bovine cartilages. the results strongly suggest that in addition to fibroblasts and smooth muscle cells, chondroblasts also synthesize elastin. some of the possible functions of elastin in elastic cartilages are discussed and the possiblity of a new type of elastin perhaps unique to cartilage is suggested. | 1977 | 72098 |
| local transfer of delayed hypersensitivity and cutaneous basophil hypersensitivity. | | 1977 | 72112 |
| preparation of radioactive basic protein of bovine nervous tissue. | | 1977 | 72176 |
| serum-ige levels and cow's-milk and egg antigens. | | 1977 | 72315 |