| [a fatal case of chronic q fever with cardiovascular involvement]. | a lethal case is reported of chronic q-fever in a patient aged 34 who regarded himself as completely healthy six months before his death. the diagnosis was made on the basis of vital investigation of blood sera in a complement-fixation test and indirect fluorescent-antibody test (antibody titers 1 : 1280 against phase 1 and 1 : 320 against phase 2 coxiella burnetii), differentiation of antibodies as distinct classes of immunoglobulins, results of pathoanatomical and microbiological investigation ... | 1995 | 8983769 |
| the role of the national institute of public health in the field of infections with natural focality. | in the post-war period the national institute of public health, later institutes of epidemiology and microbiology, headed by k. raska, ranked among famous laboratories in the world due to its priority findings and original results. research results of the institute stimulated further research not only in czechoslovakia but also abroad, in laboratories of europe and america. the authors emphasize the significance of certain results in the epidemiology and ecology of infections characterized by na ... | 1996 | 8996726 |
| cd4+ t-cell lymphopenia in q fever endocarditis. | valvular endocarditis is the most serious complication of chronic q fever, an infectious disease due to coxiella burnetii. although its pathogenesis is poorly understood, the role of the immune system has been evoked. the aim of this study was to investigate lymphocyte subsets in the peripheral blood of infected patients by analyzing the distribution of t- and b-lymphocyte subsets. since various infectious diseases have been found to be associated with modified antigen expression, we also measur ... | 1997 | 9008288 |
| q fever outbreak--germany, 1996. | in may 1996, the health department of marburg-biedenkopf in marburg, hessen, germany, was notified of a cluster of persons with high and persistent fever who resided in a rural town (rollshausen [1996 population:300]) and in five surrounding towns approximately 0.5-2.0 miles from rollshausen, in the district of lohra. serologic testing of some patients by local health authorities suggested acute q fever. in germany, q fever is a reportable disease and 27-100 cases are reported annually; during 1 ... | 1997 | 9011780 |
| autoimmune component in individuals during immune response to inactivated combined vaccine against q fever. | serum samples from 20 individuals immunized with inactivated combined vaccine (icv) against q fever and 10 individuals that received placebo were investigated on days 14, 21, 28 and 60 after immunization by isotope specific enzyme-linked immunosorbent assay (elisa) for the presence of antibodies directed to human iga, igm and igg, and their fragments (f(ab')2, fab, fc). none of the subjects that received placebo exhibited significant increase of reactivity with any of the used antigens. by contr ... | 1996 | 9014006 |
| [infectious endocarditis caused by q fever. apropos of a clinical case]. | endocarditis is a rare, but some times fatal, complication of q fever. its diagnosis is difficult and it is based on non-specific cardiac findings and a high title of phase i antibodies. the treatment is based on tetracyclines alone or in combination with cotrimoxazole, for long periods of time. the therapeutic efficacy is evaluated by the measurement of phase i antibodies, every three months. the relapses are frequent despite the long period of antibiotic therapy. we report what is probably the ... | 1996 | 9019144 |
| [serologic diagnosis of severe non-bacterial pneumonia and acquired in the community]. | the aim of this study was to evaluate the profitability of the serology, especially against streptococcus pneumoniae and chlamydia sp., in the community pneumonia with hospital admission. | 1996 | 9035712 |
| serological survey for antibodies to infectious agents in beef cattle in northern south australia. | | 1997 | 9066974 |
| serological cross-reactions between coxiella burnetii and legionella micdadei. | coxiella burnetii and legionella micdadei are both gram-negative bacteria potentially responsible for identical clinical syndromes resembling upper respiratory infections. these infections, quite common in immunocompromised patients, are usually diagnosed by serology with a microimmunofluorescence assay. we found that 34.5% of q fever patients had a significant titer of antibodies against l. micdadei. cross-reactions involved immunoglobulin g antibodies and were demonstrated by a cross-adsorptio ... | 1997 | 9067657 |
| q fever vaccination in queensland abattoirs. | outbreaks of q fever continue to be recorded in abattoirs despite a protective vaccine being available. all accredited abattoirs in queensland were surveyed to ascertain the number that conducted q fever vaccination programs. only ten of the 30 abattoirs had vaccination programs. vaccination programs were present at all abattoirs with more than 360 employees. thirty-seven per cent of abattoir employees worked at abattoirs that did not have vaccination programs. research is required into the atti ... | 1997 | 9079588 |
| analysis of immunoglobulin classes and subclasses in response to infection of balb/cj and c57bl/6j mice with coxiella burnetii. | in order to establish defined immunological parameters for q fever infection models, a microtitre enzyme-linked immunosorbent fluorescence assay (elisa) was used for the first time to analyse the humoral immune response of balb/cj and c57bl/6j mice after experimental infection with coxiella burnetii strain 'nine mile' in phase i. the experimental infection evoked a seroconversion in all mice within 10 days. typically, the immune response measured against the whole-cell antigen showed an early in ... | 1997 | 9084233 |
| seasonal variations in the presence of antibodies against coxiella burnetii in dairy cattle in hokkaido, japan. | the prevalence and seasonal variations of infection by coxiella burnetti in cattle were investigated seroepidemiologically on a farm in hokkaido, japan, by an immunofluorescent antibody test. a total of 364 serum samples from 28 cows were collected from august 1993 to october 1995 in two barns on the farm. it was found that the number of antibody-positive cows and their antibody titers were significantly elevated in winter and decreased in summer. in addition, antibodies were detectable in seroc ... | 1997 | 9087948 |
| [community acquired pneumonia. reliability of the criteria for deciding ambulatory treatment]. | to determine whether criteria for not admitting community-acquired pneumonia (cap) patients diagnosed in the emergency room are appropriate, and to characterize the symptoms, etiology and course of cap. this one-year prospective, protocol study of immunocompetent cap patients diagnosed in the emergency room of our hospital enrolled patients not considered to require hospital admission according to the recommendations of the spanish society of respiratory disease (separ). medical histories, chest ... | 1997 | 9091117 |
| [significance of causes of infectious abortion in sheep flocks in northern baden-württemberg with special reference to chlamydia psittaci]. | investigations on the reasons of infectious abortion cases in sheep flocks in northern parts of baden-wuerttemberg eludicate the wide-spreading of chlamydia psittaci (c. psittaci) and its significance as the most frequent cause of abortions in sheep. another important pathogen agent causing abortions is salmonella abortus ovis (s. abortus ovis) which could be demonstrated by using a serological elisa test. a less important role than c. psittaci and s. abortus ovis plays coxiella burnetii in abor ... | 1997 | 9092464 |
| q fever pneumonia. | pneumonia is one of the principal manifestations of q fever, a disease caused by coxiella burnetii. this bacterium can replicate only within cells, yet it is capable of surviving in the environment because it can withstand drying and substantial temperature variations. livestock, especially sheep, goats and cattle, are a major reservoir of c burnetii. the organism is transmitted to humans by direct contact with animal products, especially during parturition. aerosols have transmitted infection o ... | 1997 | 9097369 |
| [a review of q fever in the canary islands]. | | 1997 | 9102680 |
| relevance of nucleic acid amplification techniques for diagnosis of respiratory tract infections in the clinical laboratory. | clinical laboratories are increasingly receiving requests to perform nucleic acid amplification tests for the detection of a wide variety of infectious agents. in this paper, the efficiency of nucleic acid amplification techniques for the diagnosis of respiratory tract infections is reviewed. in general, these techniques should be applied only for the detection of microorganisms for which available diagnostic techniques are markedly insensitive or nonexistent or when turnaround times for existin ... | 1997 | 9105753 |
| a case of q fever endocarditis treated medically: 9 years of follow-up. | | 1997 | 9108195 |
| q fever endocarditis in israel and a worldwide review. | the worldwide epidemiology and population-based incidence of q fever endocarditis (qfe) have been less well studied than those for uncomplicated q fever. an exhaustive literature review revealed 408 patients with qfe reported between 1949 and 1994, mostly from 3 large geographic areas. underlying valvular heart disease was almost invariably present, and 38% had prosthetic valves. the most common clinical manifestations were fever and congestive heart failure. the mortality rate dropped over the ... | 1997 | 9112297 |
| the number and proportion of vgamma9 vdelta2 t cells rise significantly in the peripheral blood of patients after the onset of acute coxiella burnetii infection. | we conducted serial studies on peripheral blood lymphocytes from four patients with acute coxiella burnetii infection. these studies revealed that the proportion of gammadelta t cells in these patients significantly increased after the onset of disease (mean, 16%; range, 13%-30%) as compared with that in five healthy controls (mean, 4%; range, 0.5%-7%; p < .0055) and that in five controls with pneumonia (mean, 2%; range, 1%-3%; p < .0014). most of the gammadelta t cells from these patients expre ... | 1997 | 9114159 |
| q fever in pregnancy. | we describe a case of acute symptomatic infection with coxiella burnetii acquired between the 16th and 28th week of pregnancy. oral ciprofloxacin therapy was started on diagnosis, at the 28th week of pregnancy, but symptoms were unabated after 3 weeks treatment, suggesting persisting infection of the products of conception. caesarean section was therefore performed at 32 weeks gestation when a healthy infant was delivered, and subsequent investigations showed no evidence of transplacental spread ... | 1997 | 9120330 |
| acute q fever as a cause of acute febrile illness of unknown origin in taiwan: report of seven cases. | acute q fever, caused by coxiella burnetii has been well documented in western countries, but only recently identified in taiwan. seven cases diagnosed by indirect fluorescent antibodies to phase ii antigens of c. burnetii are reported. the patients were all men and a history of animal contact could be traced in most cases. clinical manifestations were high fever, chills, relative bradycardia and elevated serum aminotransferases. therapy with tetracycline or clarithromycin resulted in defervesce ... | 1997 | 9136520 |
| prevalence and clinical significance of igg isotype anti-beta 2-glycoprotein i antibodies in antiphospholipid syndrome: a comparative study with anticardiolipin antibodies. | to investigate the prevalence, significance, and specificity of igg isotype anti-beta 2-glycoprotein i antibodies (a-beta 2-gpi) in antiphospholipid syndrome (aps), we developed an enzyme-linked immunosorbent assay (elisa) for the detection of igg-a-beta 2-gpi and tested sera from 61 patients with autoimmune disorders (aid), 39 patients with aps and 22 patients with systemic lupus erythematosus without aps, 139 patients with various infectious diseases (hepatitis c virus infection, human immunod ... | 1997 | 9142046 |
| combined determination of coxiella burnetii-specific immunoglobulin m (igm) and iga improves specificity in the diagnosis of acute q fever. | immunoglobulin m (igm) and iga responses in patients with acute q fever were compared by enzyme-linked immunosorbent assay. an increase in both igm and iga was observed in paired sera from all 19 patients with acute q fever, and both igm and iga levels showed good correlation with complement fixation test titers. paired sera from 23 patients with infections other than q fever were also tested. igm levels were elevated in three of these patients, while iga levels were elevated in three different ... | 1997 | 9144382 |
| a study on q fever among children hospitalized for pneumonia. | the epidemiological features of q fever make it of less consequence in childhood. however, the disease is quite possible to be contracted at this age due to the great number of indirect modes of agent transmission. the disease often escapes the attention of pediatricians which results in scantiness of information about the incidence rate of q fever in childhood. the purpose of this study was to establish the q fever incidence among children in the plovdiv region and the disease's medical and soc ... | 1996 | 9145590 |
| plasmid-homologous sequences in the chromosome of plasmidless coxiella burnetii scurry q217. | chromosomal dna from coxiella burnetii scurry q217 was screened for the presence of plasmid-homologous sequences. total dna from scurry q217 was digested with noti, and the resulting dna fragments were separated by contour-clamped homogeneous electric field pulsed-field gel electrophoresis (chef-pfge). following hybridization with biotin-labeled qph1 plasmid as a probe, two dna fragments of 40 and 170 kb were identified as targets. these fragments were cloned, and subclones containing qph1-homol ... | 1997 | 9150226 |
| antibodies are generated during infection to coxiella burnetii macrophage infectivity potentiator protein (cb-mip). | antisera from rabbits immunized with formalin inactivated coxiella burnetii isolates associated with either acute (nine mile, phase i or phase ii) or chronic (priscilla) q fever showed reactivity to a c. burnetii macrophage infectivity potentiator protein (cb-mip) cloned in escherichia coli. further, antisera generated in balb/c mice after infection with live nine mile phase i or priscilla isolates also showed reactivity to cb-mip by immunoblot analysis. in addition, human serum from an individu ... | 1997 | 9159413 |
| coxiella burnetii: the 'query' fever bacterium. a model of immune subversion by a strictly intracellular microorganism. | although substantial progress occurred in the knowledge of coxiella burnetii during the past years, the pathophysiology of q fever is still obscure. emerging evidence from clinical investigations suggested that certain disorders of cell-mediated immunity play a pivotal role in q fever and especially in its chronic form. this review analyses the potential strategies that c. burnetii, a strictly intracellular pathogen, use to divert microbicidal mechanisms of macrophages and to depress protective ... | 1997 | 9167255 |
| pathologic changes during acute q fever: influence of the route of infection and inoculum size in infected guinea pigs. | as assessed by both standard histological staining and immunochemistry, intraperitoneal inoculation of c. burnetii in guinea pigs led to pathologic changes mainly in the liver, whereas intranasal inoculation led to pathologic changes mainly in the lungs. myocarditis and positive blood cultures were observed only in those animals which received the highest inoculum. we therefore conclude that both the route of infection and the size of the inoculum influence clinical expression in acute q fever. | 1997 | 9169787 |
| protein-tyrosine phosphatase activity of coxiella burnetii that inhibits human neutrophils. | supernatants prepared from disrupted coxiella burnetii possess acid phosphatase (acp) activity that apparently accounts for the inhibition of the metabolic burst of formyl-met-leu-phe(fmlp)-stimulated human neutrophils. results are presented regarding purification and biochemical-biological characterization of the acp. the highly purified enzyme, which exhibited an apparent m(r) of 91 k and optimal activity at ph 5.0, also inhibited neutrophils. the enzyme retained full activity at ph 4.5, 5.5, ... | 1996 | 9171454 |
| detection of coxiella burnetii in cow's milk by pcr-enzyme-linked immunosorbent assay combined with a novel sample preparation method. | the use of an adequate concentration of triton x-100 enhanced immunomagnetic separation of coxiella burnetii from milk. pcr-enzyme-linked immunosorbent assay (pcr-elisa) could detect coxiellas more sensitively than could conventional pcr. pcr-elisa is therefore thought to be suitable for the simultaneous assay of a large number of samples. however, the number of cows from which raw milk tested positive for coxiellas by pcr-elisa was inconsistent with that found with the antibody to coxiella by i ... | 1997 | 9172331 |
| infective endocarditis due to unusual or fastidious microorganisms. | infective endocarditis due to fastidious microorganisms is commonly encountered in clinical practice. some organisms such as fungi account for up to 15% of cases of prosthetic valve infective endocarditis, whereas organisms of the hacek group (haemophilus parainfluenzae, h. aphrophilus, and h. paraphrophilus, actinobacillus actinomycetemcomitans, cardiobacterium hominis, eikenella corrodens, and kingella kingae) cause 3% of community-acquired cases of infective endocarditis. special techniques a ... | 1997 | 9179137 |
| [community-acquired pneumonia--current status of pathogen diagnosis]. | procedures for the microbiological diagnosis of acute community-acquired pneumonia are based on the expected pathogens. although a great variety of microorganisms are able to cause community-acquired pneumonia only a few pathogens play an important role in daily practice. the most important investigations are blood cultures and sputum cultures to detect bacteria like pneumococci, haemophilus influenzae and staphylococcus aureus as well as antibody tests for mycoplasma pneumonia and chlamydia pne ... | 1997 | 9206930 |
| bacteriostatic and bactericidal activity of levofloxacin against rickettsia rickettsii, rickettsia conorii, 'israeli spotted fever group rickettsia' and coxiella burnetii. | levofloxacin, the l-isomer of ofloxacin, is approximately twice as active as ofloxacin against most gram-positive and gram-negative bacteria, and has improved intracellular pharmacokinetic and pharmacodynamic properties. the present work deals with the in-vitro activity of levofloxacin against the obligate intracellular bacteria rickettsia rickettsii, rickettsia conorii, 'israeli spotted fever group rickettsia' (israeli sfgr) and coxiella burnetii. fluoroquinolones, including ofloxacin, have pre ... | 1997 | 9222041 |
| identification of rickettsiae isolated in japan as coxiella burnetii by 16s rrna sequencing. | the 16s rrna genes of japanese coxiella isolates obtained from various sources and geographical areas were directly sequenced by dideoxynucleotide chain termination methods in which taq dna polymerase was used. the levels of sequence similarity among japanese, european, and american isolates were more than 99%, and the japanese isolates were identified as coxiella burnetii, c. burnetii strains isolated worldwide, including japan, were found to be very similar. | 1997 | 9226923 |
| evaluation of a commercial enzyme-linked immunosorbent assay for detection of immunoglobulin m antibody in diagnosis of human leptospiral infection. | the panbio leptospira immunoglobulin m (igm) enzyme-linked immunosorbent assay (elisa) is a commercially available screening test for the diagnosis of acute leptospiral infection. the ability of the test to diagnose early or recent leptospira interrogans infection was assessed by testing sera with known microagglutination test (mat) titers to serovars pomona, hardjo, copenhageni, and australis. the igm elisa detected all 41 cases of early or recent leptospiral infection (sensitivity, 100%), with ... | 1997 | 9230359 |
| [recurrent pericarditis caused by q fever]. | | 1997 | 9235062 |
| the epidemiology of q fever. | | 1997 | 9243158 |
| [a comparative study of coxiella burnetii strains differing in virulence in the intratracheal infection of guinea pigs]. | as found out in earlier investigations, after the intratracheal infection of guinea pigs with c. burnetii (the method of infection, most similar to aerogenic infection which is characteristic of coxiella under natural conditions) the maximum amount of the infective agent is accumulated in the lungs of the animals, damaging the organs of the reticuloendothelial system. the present investigation has revealed that this method of infection makes it possible differentiate c. burnetii virulent strains ... | 1997 | 9245132 |
| [zoonotic infections in the central and southern ulusy of the republic of sakha]. | the serological survey of humans, as well as agricultural animals and rodents, for the presence of zoonotic infections, was carried out. local inhabitants were shown to have contacts with the causative agents of pseudotuberculosis, enteric yersiniosis, tularemia, leptospirosis, q fever, tick-borne rickettsiosis, chlamydiosis, toxoplasmosis. the immune stratum with respect to enteric yersiniosis was found to have a greater index, while the immune strata with respect to chlamydiosis, q fever, pseu ... | 1997 | 9245139 |
| adenosine deaminase activity in the aetiological diagnosis of community-acquired pneumonia. | a prospective study was undertaken to assess the usefulness of serum adenosine deaminase (ada) activity in the aetiological diagnosis of 75 patients (mean age 58 years) with community-acquired pneumonia who required hospitalization. measurements of ada were also carried out in 35 healthy subjects (mean age 52 years). the serum ada activity in patients with typical bacterial pneumonia (tbp) was 21 +/- 7 iu/l and in controls 22 +/- 9 iu/l. in 43 patients with atypical pneumonia (ap), ada levels (4 ... | 1997 | 9255891 |
| specific detection of coxiella burnetii through partial amplification of 23s rdna. | a previously published sequence of the 23s rrna gene of coxiella burnetii has been reported to contain an intervening sequence of 444 base pairs (bp). the sequence information on the intervening sequence and the 23s rrna gene was exploited to develop a specific pcr-based assay for c. burnetii. a primer set was designed that amplified a 477-bp fragment encompassing part of the intervening sequence and part of the 23s rdna. from all of nine c. burnetii strains tested, a fragment of the expected si ... | 1997 | 9258533 |
| study of the 16s-23s ribosomal dna internal spacer of coxiella burnetii. | the complete 16s-23s ribosomal dna (rdna) internal transcribed spacer (its) of 22 isolates of the obligate intracellular bacterium coxiella burnetii, the agent of q fever, were amplified by the polymerase chain reaction (pcr) and sequenced using an automated laser fluorescent dna sequencer. the its measured 497 base pairs (bp) and encoded isoleucine-trna and alanine-trna. the comparison of the sequence alignments of the 22 c. burnetii strains revealed very high levels of sequence similitary (> 9 ... | 1997 | 9258555 |
| exposure of cats in southern africa to coxiella burnetii, the agent of q fever. | recently, the domestic cat has been implicated in numerous outbreaks of q fever in humans. to determine if cats in southern africa are infected with the agent of q fever we tested sera from cats in south africa and zimbabwe by indirect fluorescence for antibodies reactive with phase ii coxiella burnetii antigen (nine mile strain). reactive antibodies were detected at titres of > or = 1/40 in sera from cats in south africa (1/52, 2%) and zimbabwe (15/119, 13%). our results indicate that cats in s ... | 1997 | 9258556 |
| a salmonella typhimurium genetic locus which confers copper tolerance on copper-sensitive mutants of escherichia coli. | three distinct clones from a salmonella typhimurium genomic library were identified which suppressed the copper-sensitive (cu(s)) phenotype of cutf mutants of escherichia coli. one of these clones, pcutfs2, also increased the copper tolerance of cuta, -c, and -e mutants, as well as that of a lipoprotein diacylglyceryl transferase (lgt) mutant of e. coli. characterization of pcutfs2 revealed that the genes responsible for suppression of copper sensitivity (scs) reside on a 4.36-kb dna fragment lo ... | 1997 | 9260936 |
| seroepidemiology of rickettsia typhi, spotted fever group rickettsiae, and coxiella burnetti infection in pregnant women from urban tanzania. | immunofluorescent antibody (ifa) testing was performed on sera drawn from 150 pregnant women in the port city of dar es salaam, tanzania. prevalence of antibodies to rickettsia typhi was 28%, higher than in any of the 12 other african countries in which serosurveys using ifa testing have been performed. seroprevalence of antibodies to spotted fever group rickettsiae antigens was 25.3%, comparable with that found in other sub-saharan countries endemic for amblyomma ticks. only 4.7% of women were ... | 1997 | 9288814 |
| [frequency and cost of health problems in swiss dairy cows and their calves (1993-1994)]. | between july 1993 and july 1994 morbidity and management information related to dairy cows and their calves up to the age of 8 weeks were recorded in 113 randomly selected dairy herds. also recorded were any costs incurred through disease and prevention. blood and faeces were analysed with respect to selected pathogens. the health problems most frequently diagnosed in cows were reproductive and udder diseases. calves suffered most often from diarrhea, omphalitis and pneumonia. the directly disea ... | 1997 | 9297231 |
| isolation of coxiella burnetii from bull semen. | previous observations on the dissemination of coxiella burnetii between laboratory animals strongly support the hypothesis of venereal transmission. serum and semen samples, from 57 bulls used for artificial insemination, were assayed for specific c. burnetii phase ii antibodies and the presence of the organism respectively. viable c. burnetii were detected in the semen of seropositive bulls. these findings indicate the possibility of sexual transmission of c. burnetii between cattle and further ... | 1997 | 9300554 |
| [frequency and etiology of calf losses and calf diseases in cow-calf farms. iii. seroprevalence of selected diseases and prevalence of endoparasites and weaning age]. | in a cross sectional study in 38 cow-calf farms blood and fecal samples were taken from 207 calves at an age of 8-10 months and their darms, in order to determine the seroprevalence of leptospira hardjo, coxiella burnetii, toxoplasma gondii and the presence of the bvd-antigen as well as the prevalence of endoparasites. the study was conducted in fall 1994 at the end of the grazing period. eggs or larvaes of gastrointestinal helminths were detected in 74% of calves that were dewormed before the g ... | 1997 | 9303850 |
| [serological study of rickettsia infections in niamey, niger]. | rickettsioses is a possible alternative to presumptive diagnosis of malaria. a serologic study was carried out in 1994 to determine the prevalence of rickettsioses in children under 5 years of age from three different areas of niamey, niger. indirect immunofluorescent assays using the micromethod were performed with antigens for rickettsia conori, rickettsia mooseri, and coxiella burneti. results were read from a positive threshold of 1/160 up to 1/640. out of a randomized population of 177 chil ... | 1997 | 9304008 |
| coxiella burnetii endocarditis in a child. | | 1997 | 9306492 |
| relationship between pathogenicity of coxiella burnetii isolates and gene sequences of the macrophage infectivity potentiator (cbmip) and sensor-like protein (qrsa). | coxiella burnetii, the q fever agent, is an obligate intracellular bacterium and survival in phagolysosomes is an important virulence factor. the present study was performed to determine the relationship between its pathogenicity and genes related to its survival in macrophages, i.e. macrophage infectivity potentiator and q fever agent regulatory sensor-like protein. the sequence similarity was more than 99% among japanese, european and american strains, and no relationship was found between pat ... | 1997 | 9311117 |
| [aseptic meningitis caused by coxiella burnetti]. | | 1997 | 9312290 |
| copy number of the 16s rrna gene in coxiella burnetii. | coxiella burnetii is an obligate intracellular bacterium with a doubling time of 5-7 hours. chromosomal dna from c. burnetii was digested with various restriction enzymes previously determined to not cut within the genomic 16s rrna gene, or with a combination of these noncutting enzymes in conjunction with afliii, a restriction enzyme that cuts twice within the 16s rrna gene. restriction fragments were resolved electrophoretically and probed with a radiolabeled dna fragment containing the 3' afl ... | 1997 | 9324222 |
| endosymbionts of ticks and their relationship to wolbachia spp. and tick-borne pathogens of humans and animals. | the presence, internal distribution, and phylogenetic position of endosymbiotic bacteria from four species of specific-pathogen-free ticks were studied. these included the hard ticks ixodes scapularis (the black-legged tick), rhipicephalus sanguineus (the brown dog tick), and haemaphysalis longicornis and the african soft tick ornithodoros moubata. pcr assays for bacteria, using two sets of general primers for eubacterial 16s and 23s rrna genes (rdnas) and seven sets of specific primers for wolb ... | 1997 | 9327557 |
| reassessment of the taxonomic position of rickettsiella grylli. | we determined the 16s rrna gene sequence of rickettsiella grylli, an intracellular parasite of gryllus bimaculatus and related species of crickets. phylogenetic inferences made from alignment of this sequence with the sequences of other bacteria demonstrated that r. grylli is most closely related to coxiella burnetii and legionella species in the gamma subclass of the phylum proteobacteria. r. grylli was previously thought to be related to members of the order rickettsiales, but the representati ... | 1997 | 9336939 |
| prevalence of q fever in a rural practice. | q fever is a world-wide condition caused by the rickettsia coxiella burnetii. it appears more prevalent in agrarian communities and may have serious sequelae. | 1997 | 9347458 |
| comparative efficacy of a coxiella burnetii chloroform:methanol residue (cmr) vaccine and a licensed cellular vaccine (q-vax) in rodents challenged by aerosol. | q fever is an acute and self-limited febrile illness caused by the obligate intracellular bacterium coxiella burnetii. while phase i cellular q fever vaccines are efficacious in humans, vaccination of immune individuals may result in sterile abscesses and granulomas. the chloroform:methanol residue vaccine (cmr) was developed as a safer alternative. the efficacy of a licensed phase i cellular vaccine (q-vax) was compared with that of cmr vaccine in a/j mice and hartley guinea pigs challenged wit ... | 1997 | 9364683 |
| codon usage and nucleotide composition in coxiella burnetii. | coxiella burnetii, the causative agent of q fever, is an obligate intracellular bacterium. with the development of molecular biology techniques, there have been increasing efforts on gene cloning and other genetic analyses of this organism. in this report, we tabulate the codon usage (cu) and nucleotide (nt) co-occurrence in c. burnetii, based on available nt sequence data. the average g+c content of the c. burnetii genome is 42.4%, where the g+c content is 42.7% for the chromosome and 38.7% for ... | 1997 | 9370279 |
| occupational infection in an offal porter: a case of q fever. | this case report describes the job activities of an offal porter who developed q fever while processing livers from sheep. the diagnosis was confirmed by an increase in specific serial antibody titre. the main clinical features were anorexia, nausea, headache, pyrexia and elevated gamma-glutamyl transferase. twenty-four cases of occupationally-acquired q fever were noted by the communicable diseases surveillance centre (cdsc) between 1984 and 1994. this case report has an important feature in th ... | 1997 | 9374073 |
| [q fever in patients with hiv infection]. | | 1997 | 9379737 |
| evaluation of the high-density agglutination test for coxiella burnetii antibodies in animals. | the usefulness of the high-density particle agglutination (hdpa) test as a potential tool for the detection of anti-coxiella burnetii antibodies in animal sera was studied by using 619 cow, 589 dog, and 150 cat serum samples and antisera from rabbits, guinea pigs, and mice. the sensitivity and specificity of the test versus those of the reference microimmunofluorescence test were determined at two different threshold titer values. at the cutoff value of 1:16, the sensitivities of the hdpa test f ... | 1997 | 9384288 |
| [q fever endocarditis and bicuspid aortic valve]. | q fever is caused by the rickettsia coxiella burnetti, an obligate intracellular bacterium acquired by inhalation of infected dust from subclinically infected animals. q fever may be acute or chronic; the chronic form mostly presents as endocarditis. immunocompromised states and underlying heart disease are the most important risk factors. usually the symptoms of q fever endocarditis are nonspecific and diagnosis is often established very late. new criteria for diagnosis include a single blood c ... | 1997 | 9418357 |
| choroidal neovascularization in the course of q fever. | | 1997 | 9428021 |
| clinical evaluation of a new pcr assay for detection of coxiella burnetii in human serum samples. | a nested pcr method was developed for the detection of coxiella burnetii in human serum samples. two pairs of oligonucleotide primers were designed to amplify a 438-bp fragment of the com1 gene encoding a 27-kda outer membrane protein of c. burnetii. the primers amplified the predicted fragments of 21 various strains of c. burnetii but did not react with dna samples from other microorganisms. the 438-bp amplification products could be digested with restriction enzymes sspi and sali. the utility ... | 1998 | 9431924 |
| [q-fever imported into norway]. | q fever is an important zoonosis that occurs throughout the world. in contrast to most other european countries, there has been no evidence of endemic q fever in norway up to now. the disease is caused by coxiella burnetii, a rickettsia-like bacterium. humans are infected mainly by inhalation of contaminated aerosols from cattle, sheep and goats. clinical manifestations are protean, ranging from asymptomatic infection to life-threatening endocarditis. in this article we present the first four ca ... | 1997 | 9441420 |
| differentiation of coxiella burnetii by sequence analysis of the gene (com1) encoding a 27-kda outer membrane protein. | the gene (com1) encoding a 27-kda outer membrane protein in 21 strains of coxiella burnetii from a variety of clinical and geographical sources was sequenced for strain differentiation. the com1 gene was highly conserved among all the strains tested but there were several differences in nucleotide and deduced amino acid sequences. based on the com1 gene-specific nucleotides and deduced amino acids, the 21 strains were divided into four groups. group 1 contained 14 strains originating from ticks, ... | 1997 | 9444329 |
| coxiella burnetii infection of aneurysms or vascular grafts: report of seven cases and review. | the most frequent clinical presentation of chronic q fever is endocarditis, although infections of aneurysms and vascular prostheses have also been described. we report seven new cases of coxiella burnetii infection of aneurysms or vascular grafts. we also review the literature and compare our cases with the six previously reported cases. this study demonstrated the lack of specific symptoms associated with this disease. moreover, prospectively, in an attempt to reevaluate the incidence of q fev ... | 1998 | 9455519 |
| an explosive outbreak of q-fever in jedl'ové kostol'any, slovakia. | an explosive epidemic of q-fever that occurred at jedl'ové kostol'any (nitra district) in april 1993, had an unusual mode of transmission, unprecedented in slovakia. the submitted case-reports can be very instructive for both health workers and the lay public. the bulk of infection was spread in the local pub through contaminated garments of animal attendants assisting abortions and births of goats in a large capacity breeding centre of gemersan co. by their repeated visits to the local pub the ... | 1997 | 9457416 |
| [the use of atomic-force microscopy for the analysis of microbiological objects]. | atomic force microscopy (afm) was used for the analysis of rickettsiae and viruses. the specificity of interaction was evaluated on the basis of the adsorption of the analyzed antigen on the polymer-antibody film. the film was formed and transferred onto highly oriented pyrolytic graphite (hopg) by the method of langmuir-schaefer with the use of amphiphilic polymers, alkylated polyethyleneimines. according to the data of afm, polymer-antibody film was 10-30 nm thick and had ruptures, uneven surf ... | 1997 | 9460856 |
| [the characteristics of the spread of coxiella burnetii in the carpathian region]. | the paper presents data of the long-term (1975-1994) study of q fever in carpathian region [correction of forecarpathia] (ivano-frankivs'k and chernivtsi provinces) and dynamics of epidemiologic manifestations of this disease during the recent years. it is shown that after q fever outbreaks in 1975-1977, people sick for it have been recorded sporadically, and a considerable decrease of epidemiological manifestations of coxiella burnetii circulation is observed in the late years. this is accompan ... | 1997 | 9480017 |
| q fever tricuspid valve endocarditis. | q fever is a zoonosis caused by coxiella burnetii. the most frequent clinical expression of the chronic form is a bacterial culture negative aortic or mitral endocarditis. a case of tricuspid valve endocarditis due to c burnetii is described, with a favourable outcome after treatment with doxycycline and hydroxychloroquine. | 1997 | 9487972 |
| q fever trilogy morbidity--deadends--stark choices. | | 1997 | 9489180 |
| a review of q fever in australia 1991-1994. | q fever continues to be an important disease in australia. despite the development of an effective vaccine that has been commercially available since 1989, the number of cases notified has continued to increase. this study reviewed national notifications of q fever between 1991 and 1994, together with demographic, socioeconomic and occupational information, to investigate temporal and spatial disease patterns. based on notification data, q fever can be considered primarily a disease of adult mal ... | 1997 | 9489189 |
| pneumococcal community-acquired pneumonia in 148 hospitalized adult patients. | in a previous prospective study, streptococcus pneumoniae was identified as the causative agent in 148 (42.8%) of 346 adult patients hospitalized over the course of one year with community-acquired pneumonia (cap) in the soroka medical center, beer-sheva, israel. the present study characterizes those cases in which streptococcus pneumoniae was the only pathogen and those in which additional etiological agents were identified. pneumococcal cap was diagnosed by standard blood cultures or positive ... | 1997 | 9495665 |
| herd-level coxiella burnetii seroprevalence was not associated with herd-level breeding performance in czech dairy herds. | q fever was studied on a large agricultural farm in northern moravia, czech republic. antibodies to coxiella burnetii were ascertained by a complement fixation test. titre of 8 or higher was considered as positive. the seroprevalences in cows (each cow was examined only once immediately after drying-off during a one-year period) from 14 different herds was between 4 to 19%. no significant correlation between seroprevalence levels and fertility characteristics in the cow herds was found. of a tot ... | 1998 | 9500180 |
| public health hazards from small ruminant meat products in europe. | foodborne diseases, in particular those related to meat and meat products, have recently become a matter of great public concern. sheep and goat meat can transmit infections and diseases either through handling during preparation procedures or as a result of ingestion by the consumer. the authors highlight the second route of contamination in relation to meat and meat products from small ruminants in european countries. among the most important diseases transmitted by mutton and goat meat, toxop ... | 1997 | 9501355 |
| acute glomerulonephritis associated with acute q fever: case report and review of the renal complications of coxiella burnetii infection. | we report a case of acute glomerulonephritis associated with acute q fever. an abattoir worker with a nonspecific febrile illness and pneumonia and abnormal liver function test results developed hematuria, proteinuria, and acute renal failure that resolved with appropriate antimicrobial therapy. renal biopsy demonstrated diffuse proliferative and exudative glomerulonephritis. serological tests confirmed recent infection with coxiella burnetii, with a fourfold rise in the titer of phase ii antibo ... | 1998 | 9502456 |
| determination of coxiella burnetii rpob sequence and its use for phylogenetic analysis. | the nucleotide sequence of the rpob, encoding the beta-subunit of rna polymerase of the obligate intracellular bacterium coxiella burnetii, was determined using a polymerase chain reaction amplification and direct sequencing methodology. comparison between c. burnetii and other eubacterial rpob sequences indicated sequence similarity ranging from 53.6% to 67.6%. coxiella burnetii rpob consists of 4128 base pairs with a 45.3% gc content encoding 1375 amino acids with a calculated molecular mass o ... | 1998 | 9511749 |
| [tropical animal and human rickettsial infections]. | advances in molecular biology have provided tools that have greatly clarified our knowledge of rickettsial diseases. most rickettsias have been reclassified in the alpha subgroup of proteobacteria. four groups of rickettsias have been identified: the spotted fever group, the typhus group, ehrlichia, and bartonella. although still considered as a rickettsia, coxiella burnetti, the agent causing q fever, has been reclassified separately in the gamma subdivision of proteobacteria. recognition of ri ... | 1997 | 9513175 |
| isolation of coxiella burnetii from the vagina of feline clients at veterinary clinics. | | 1998 | 9524952 |
| antigenic characteristic of the lipopolysaccharides of coxiella burnetii isolates. | lipopolysaccharides (lpss) of 8 isolates of coxiella burnetii from a variety of clinical and geographical sources could be divided into four groups based on molecular heterogeneity in silver-stained sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) profiles in the region of the 10 to 17 kda. the lipopolysaccharide of group 1 was identified on isolates from acute q fever patient, milk and tick. the three remaining groups were primarily found on isolates from human cases of chro ... | 1998 | 9524957 |
| detection of coxiella burnetii from dust in a barn housing dairy cattle. | we attempted to detect coxiella burnetii in dust samples collected from a barn housing dairy cattle by the polymerase chain reaction (pcr) method. ten dust samples (five from ventilation fans and five from crossbeams) were collected from two areas in a barn on a farm near sapporo, hokkaido. c. burnetii was detected in 5 of the 10 dust samples. it was believed that aerial contamination by c. burnetii occurred in the barn. | 1998 | 9525780 |
| major tick-borne diseases of sheep and goats in the sudan. | tick-borne diseases of sheep and goats have not been thoroughly investigated in the sudan. heartwater, the most important of the group, was reported only in the early '60s and malignant theileriosis of sheep in the mid '80s. other tick-borne diseases of sheep and goats like anaplasmosis, q fever, nairobi sheep disease and babesiosis are expected to be present although the vector which transmit nairobi sheep disease, rhipicephalus appendiculatus is confined to a very narrow stretch on the souther ... | 1997 | 9530699 |
| genotypic and phenotypic characterization of two swedish isolates and two prototypic strains of coxiella burnetii. | two swedish isolates of coxiella burnetii and the two prototype strains of the species, nine mile and priscilla, were characterized with regard to their multiplication and cytopathic effect on bgm cells and by pcr-based amplification of repetitive element dna and the c. burnetii-specific plasmids qph1 and qprs. moreover, 1330 bp of each 16s rrna gene were sequence-determined. all four strains multiplied at virtually the same rate and displayed the same type of vacuoles in the bgm cells. genetic ... | 1998 | 9544785 |
| occupational risks of zoonotic infections in dutch forestry workers and muskrat catchers. | lyme borreliosis (lymeb), hemorrhagic fever with renal syndrome (hemofrs), lymphocytic choriomeningitis (lymphocm), tick-borne encephalitis (tickbe), q fever (qfever), and weil's leptospirosis (lepto) are known to be occupational diseases for rural outdoor workers. we investigated the occupational infection risks for these diseases in greater detail. antibodies to these agents were assessed in 312 forestry workers and muskrat catchers, and in 356 matched office workers. three levels of occupatio ... | 1998 | 9556168 |
| an outbreak of sheep-associated q fever in a rural community in germany. | in spring 1996, an outbreak of q fever occurred among residents of a rural town (population: 300) in germany. a retrospective cohort study was conducted to ascertain the extent of the outbreak and to assess potential risk factors for illness. in july 1996, all residents > or =15 years received a self-administered questionnaire and were offered coxiella burnetii antibody testing. residents were considered to have probable q fever if they had a positive result for igm c. burnetii antibodies by eli ... | 1998 | 9556180 |
| membrane association of active plasmid partitioning protein a in escherichia coli. | qsopa and sopa, proteins essential for stable maintenance of low copy number plasmids and encoded on plasmid qph1 of coxiella burnetii and the f plasmid of escherichia coli, respectively, are shown to be membrane associated using three independent approaches: isolation of hybrid protein a-phoa proteins that display phoa (bacterial alkaline phosphatase) activity indicating a periplasmic location, biochemical fractionation by flotation gradient centrifugation, and subcellular localization by immun ... | 1998 | 9556623 |
| the effect of activation of the lactoperoxidase system and souring on certain potential human pathogens in cows' milk. | conventional methods of ensuring the safety and soundness of cows' milk for human consumption, such as pasteurisation, are not always practical in poor socioeconomic conditions or in rural communities that lack modern amenities. activation of lactoperoxidase (lp) system and souring of milk were investigated as potential alternative methods to sustain the safety of milk by inhibiting certain microorganisms with known pathogenic potential. the activation of the lp-system inhibited the growth of st ... | 1997 | 9561497 |
| high seroprevalence of coxiella burnetii infection in eastern cantabria (spain). | coxiella burnetii is the causative agent of q fever, a worldwide zoonosis which shows a wide diversity of clinical manifestations. the objective of this seroepidemiological study was to estimate the prevalence of antibodies to c. burnetii in a representative population of the eastern area of the cantabrian community (northern spain). | 1998 | 9563708 |
| [site of care provision, etiology and treatment of community-acquired pneumonia in palma de mallorca]. | it is not common that community-acquired pneumonias studies include patients non treated in hospital. the objectives were: to determine the cases managed in the ambulatory setting; to describe the clinical features; to identify the aetiological agents, and to describe the treatment, comparing inpatients with outpatients. | 1998 | 9567255 |
| antigenic characteristics of polypeptides of coxiella burnetii isolates. | eighteen coxiella burnetii strains from a variety of clinical and geographical sources were screened for antigenic variation of polypeptides by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (sds-page) coupled with coomassie brilliant blue (cbb) staining or immunoblotting. these polypeptide profiles showed the greatest variability in the region from 33 to 8.1 kda. such differences in the antigenicity of the polypeptides were also recognized by immunoblotting with 15 various mouse anti ... | 1998 | 9572039 |
| coxiellosis in domestic and wild birds from japan. | serological evidence of infection with coxiella burnetii was found in 41 (2%) of 1,951 domestic birds and in 167 (19%) of 863 wild birds from 17 and 5 prefectures in japan, respectively, by microagglutination (ma) test. the bacteriological evidence of the infection was found in 17 (41%) of 41 domestic birds and 37 (22%) of 167 wild birds by the nested polymerase chain reaction (pcr). in addition, c. burnetii was isolated from five each of serum, spleen and fecal specimens from five jungle crows ... | 1998 | 9577778 |
| post-infection fatigue syndrome following q fever. | in 1989, 147 individuals in the west midlands, uk, were infected with q fever. five years later, following anecdotal reports of fatigue, we used a questionnaire-based case-control study to determine the prevalence of chronic fatigue syndrome symptoms in this group. replies from 71 patients were compared with those from 142 age- and sex-matched controls. increased sweating (52.9% vs. 31.6%, p = 0.006), breathlessness (50.7% vs. 30.6%, p = 0.006), blurred vision (34.3% vs. 17.8%, p = 0.016) and un ... | 1998 | 9578893 |
| [coxiella burnetti infectious endocarditis. apropos of a case]. | chronic forms of q fever (endocarditis) are rare, but are responsible for severe and desperately recurrent infections, resulting in multiple valve replacements with a reserved prognosis. the authors report the case of a 35-year-old patient with a known history of rheumatic fever, who developed blood culture negative infectious endocarditis on a mitral bioprosthesis. the diagnosis of q fever was based on serological arguments. despite long-term antibiotic therapy, serology remained strongly posit ... | 1997 | 9587433 |
| the relationship between fatigue, psychological and immunological variables in acute infectious illness. | the aim of this paper is to explore the longitudinal relationships between physical and psychological symptoms and immunological factors following acute infective illnesses. | 1998 | 9588296 |
| [q fever with endocarditis: clinical presentation and serologic follow-up of 21 patients]. | the purpose of this study was to analyse the clinical and serological follow-up in 21 patients with q fever endocarditis in switzerland from 1981 to 1993. | 1998 | 9592893 |
| molecular characterization of coxiella burnetii isolates. | restriction fragment length polymorphism (rflp) was used for the differentiation of 80 coxiella burnetii isolates derived from animals and humans in europe, usa, africa and asia. after noti restriction of total c. burnetii dna and pulsed field gel electrophoresis (pfge) 20 different restriction patterns were distinguished. the index of discrimination for this typing system was 0.86. comparison and phylogenetic analysis of the different rflp patterns revealed evolutionary relationships among grou ... | 1998 | 9593485 |
| comparative study of the clinical presentation of legionella pneumonia and other community-acquired pneumonias. | the aim of this study was to compare the clinical, biological, and radiologic features of presentation in the emergency ward of community-acquired pneumonia (cap) by legionella pneumophila (lp) and other community-acquired bacterial pneumonias to help in early diagnosis of cap by lp. three hundred ninety-two patients with cap were studied prospectively in the emergency department of a 600-bed university hospital. univariate and multivariate analyses were performed to compare epidemiologic and de ... | 1998 | 9596294 |
| [young man with bilateral pulmonary infiltrates and liver involvement]. | | 1998 | 9611880 |