| a new immunomodulating compound (as-101) with potential therapeutic application. | there has been interest in the potential of synthetic compounds to modify immune responses by imitation of cytokine action. direct administration of interleukin 2 (il-2) in conjunction with adoptive transfer of lymphokine activated killer cells has been used in the treatment of cancer, but there are toxic effects resulting from the high doses of il-2 required. we have developed a new synthetic compound, ammonium tri-chloro(dioxoethylene-o,o'-)tellurate (as-101), which has immunomodulating proper ... | 1987 | 3118216 |
| gonadotropin regulation of tissue-type and urokinase-type plasminogen activators in rat granulosa and theca-interstitial cells during the periovulatory period. | plasminogen activators (pas) are believed to be involved in ovulation. because both tissue-type plasminogen activator (tpa) and urokinase-type plasminogen activator (upa) are secreted by cultured rat granulosa cells, we have examined the activities of these proteins in ovarian homogenates as well as granulosa and theca-interstitial (ti) cells during gonadotropin-induced ovulation. immature rats were injected with 20 iu pregnant mare serum gonadotropin (pmsg) to initiate follicle development, fol ... | 1987 | 3121412 |
| evaluation of the 11co2 positron emission tomographic method for measuring brain ph. i. ph changes measured in states of altered pco2. | the 11co2 method for measuring local brain ph with positron emission tomography (pet) has been experimentally evaluated, testing the adequacy of the kinetic model and the ability of the method to measure changes in brain ph. plasma and tissue time/activity curves measured during and following continuous inhalation of 11co2 were fit with a kinetic model that includes effects of tissue ph, blood flow, and fixation of co2 into compounds other than dissolved gas and bicarbonate ions. for each of ten ... | 1987 | 3121647 |
| lactate dehydrogenase and malate dehydrogenase of sertoli cells in rats. | lactate-dehydrogenase (ldh) and malate-dehydrogenase (mdh) activities and isozyme patterns of maturing sertoli cells were investigated in cultured cells obtained from 2-5-week-old rats. both enzymes, and more strikingly ldh, increased between 2 and 3 weeks of age. ldh isozyme complement included all five isozymes and, in the more mature rats, had a shift toward the isoforms rich in subunit. these data are consistent with an increasing production of lactate/pyruvate and with a finer regulation of ... | 1987 | 3122678 |
| polymicrobial septicemia due to shigella flexneri and pseudomonas aeruginosa: first report. | an invasion of the bloodstream by members of the genus shigella is extremely rare. when it does occur, the etiologic agent is usually a single organism. a report of the first case of shigella bacteremia that was complicated by pseudomonas aeruginosa sepsis is reported. | 1987 | 3123706 |
| effects of cadmium on human platelet reactions. | human platelets incubated with cd2+ took up the cation slowly, and the uptake was speeded up by ionophore a23187. the capacity of human platelets to accumulate cd2+ was large, equivalent to 10 nmol cd2+ per mg protein. the effects of cd2+ on protein phosphorylation and serotonin release of human platelets were studied. washed platelets incubated with cd2+ showed a general increase in protein phosphorylation concurrent with a slow release of serotonin. in the presence of ionophore a23187, however ... | 1987 | 3125819 |
| estimation of pyruvate dehydrogenase (e1) activity in human skeletal muscle; three cases with e1 deficiency. | pyruvate dehydrogenase (e1) catalyzes the rate-limiting step of the pyruvate dehydrogenase complex. since e1 activity of human muscle tissue is low, a sensitive method is needed for diagnostic purposes. measurement of 14co2 production from [1-14c]pyruvate provides a specific and sensitive assay for measuring e1 activity. we use as artificial electron acceptor dichlorophenolindophenol (dcpip) instead of the often applied ferricyanide. the method can be applied to small muscle samples obtained by ... | 1988 | 3127087 |
| feline aortic thromboemboli. | | 1988 | 3128904 |
| inhibition of beta-bungarotoxin binding to brain membranes by mast cell degranulating peptide, toxin i, and ethylene glycol bis (beta-aminoethyl ether)-n,n,n',n'-tetraacetic acid. | the presynaptically active snake venom neurotoxin beta-bungarotoxin (beta-butx) is known to affect neurotransmitter release by binding to a subtype of voltage-activated k+ channels. here we show that mast cell degranulating (mcd) peptide from bee venom inhibits the binding of 125i-labeled beta-butx to chick and rat brain membranes with apparent ki values of 180 nm and 1100 nm, respectively. the mechanism of inhibition by mcd peptide is noncompetitive, as is inhibition of 125i-beta-butx binding b ... | 1988 | 3130091 |
| effects of growth hormone-releasing hormone and corticotropin-releasing hormone on the release of thyrotropin-releasing hormone from the rat hypothalamus in vitro. | effects of growth hormone-releasing hormone (grh) and corticotropin-releasing hormone (crh) on the release of immunoreactive thyrotropin-releasing hormone (ir-trh) from the rat hypothalamus in vitro were studied. the rat hypothalamus was incubated in medium 199 with 1.0 mg/ml of bacitracin (ph 7.4) for 20 min. the amount of ir-trh release into the medium was measured by radioimmunoassay. the ir-trh release from the rat hypothalamus was inhibited significantly in a dose-related manner with the ad ... | 1987 | 3131150 |
| death despite malaria prophylaxis. | | 1988 | 3133035 |
| processing of neurofilament proteins from perikaryal to axonal type. | in previous studies, neuronal cell bodies, excised by hand from bovine spinal ganglia, were analyzed and heterogeneous intermediate and high molecular weight neurofilament proteins that differed in electrophoretic mobility from their axonal counterparts were demonstrated. in the present experiment, intermediate and high molecular weight neurofilament proteins of the axonal type were treated with alkaline phosphatase, and neurofilament proteins enriched in perikaryal type proteins were labeled wi ... | 1988 | 3137483 |
| [dysenteric arthritis--a case-related observation]. | | 1988 | 3140351 |
| expression of four virulence antigens of shigella flexneri is positively regulated at the transcriptional level by the 30 kilodalton virf protein. | on the virulence plasmid of shigella flexneri the virg region required for cell-to-cell spread of the bacterium encodes a 130 kilodalton (kd) antigen and region-2 essential for the bacterial invasion of epithelial cells encodes 57, 43 and 39 kd antigens. the expression of these four antigens is positively regulated by the 30 kd protein encoded by virf, whose nucleotide sequence had been determined and which was previously found to be essential for virulence. an approximately 3.8 kilobase (kb) rn ... | 1988 | 3141742 |
| short- and long-term effects of a single dose of bisphosphonates on retinoid-induced bone resorption in thyroparathyroidectomized rats. | the inhibitory effect of a single subcutaneous (s.c.) dose of three different bisphosphonates (bps)--4-amino-1-hydroxybutylidene-1,1-bisphosphonate (ahbubp), 2-(2-pyridinyl)-ethylidene-1,1-bisphosphonate (2-pebp), and dichloromethylene-bisphosphonate (cl2mbp)--was studied in a model of retinoid-induced bone resorption, which consists of assessing the hypercalcemic effect of the arotinoid ro 13-6298 given s.c. for three days in thyroparathyroidectomized (tptx) rats. the retinoid was given on day ... | 1988 | 3145796 |
| plasmid dna survey of clinically isolated shigella strains in shanghai area. | | 1988 | 3145835 |
| effects of maternal exposure to polychlorobiphenyls (pcbs) on f1 generation behavior in the rat. | the effect of fenclor 42 (pcb) exposure of female rats (fischer 344 strain) was studied through assessment of the behavioral development of their f1 progeny. female rats were exposed to pcb according to the following treatment schedule: (a) (5 days) 2 weeks prior to mating, (b) during gestation (days 6-15 of pregnancy), (c) during lactation (days 1-21 after delivery). behavioral endpoints of motor reflexes, motor coordination, activity (preweaning behaviors), and learning (postweaning behavior) ... | 1988 | 3146520 |
| scopolamine produces environment-specific conditioned activity that is not blocked by pimozide in rats. | a classical conditioning paradigm was employed to determine if the stimulant effects of the anticholinergic scopolamine could show conditioning. in experiment 1 rats had 12 60-min pairings of scopolamine (1.0 or 8.0 mg/kg, ip) with a distinctive environment that monitored horizontal and vertical activity. experimental (paired) groups received the drug 30 min prior to each session, whereas control (unpaired) groups received saline. following each session the paired groups were injected with salin ... | 1988 | 3146771 |
| effects of interferon alpha on performance in man: a preliminary report. | the effects of three doses of interferon alpha (1.5 mu, 0.5 mu and 0.1 mu) on performance were studied. the injection of 1.5 mu ifn produced symptoms and performance changes which closely resembled those found in volunteers with influenza. specifically, volunteers were slower at responding when they were uncertain when a target stimulus would appear, but were not impaired on a pursuit tracking task or syntactic reasoning task. the results suggest that interferon-induced changes in cns function p ... | 1988 | 3146777 |
| [the effect of long-term exposure to gamma rays (6,7 gy) on the activity of monoamine oxidase in the hypothalamus of sheep during the anestrus period]. | | 1988 | 3146833 |
| the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions of boars. | yorkshire boars were used to evaluate the influence of duration of photoperiod and hemicastration on growth and testicular and endocrine functions. at 10 wk of age, 5 hemicastrate (hc) and 5 intact (i) boars were assigned to either 8 or 16 hr of light daily until 6 mo of age. body weights were recorded biweekly throughout the experiment. venous cannulae were placed in all boars at 6 mo of age, and serum was collected at 30 min intervals from 0800 to 2000 hr. gonadotropin releasing hormone (gnrh) ... | 1988 | 3147165 |
| carbonic anhydrase isoenzymes in the caecum and colon of normal and germ-free rats. | histochemical and immunocytochemical methods were used to study the presence of carbonic anhydrase (ca) isoenzymes in the caecum and colon of normal and germ-free rats. very high enzyme activity was demonstrated by histochemistry in the caecum and proximal colon of normal rats, while the activity decreased in the distal colon. very strong immunostaining for the isoenzyme ca i was found in the cytoplasm of surface cells and upper gland cells in the caecum and colon of normal rats. in the distal c ... | 1988 | 3147569 |
| in vivo measurement of intracellular ph in human brain during different tensions of carbon dioxide in arterial blood. a 31p-nmr study. | the effect of changes in carbon dioxide tension in arterial blood upon intracellular ph in brain tissue was studied in seven healthy volunteers, aged 22-45 years. the ph changes were monitored by use of 31p nuclear magnetic resonance spectroscopy, performed on a whole-body 1.5 tesla siemens imaging system. the measurements were carried out during hyperventilation and with the subject breathing atmospheric air containing 5 vol. % and 7 vol. % carbon dioxide. intracellular ph increased significant ... | 1988 | 3147578 |
| the development of immunological relationship between mother and fetus under physiological and pathological conditions. | on the basis of results of our research and review of literature, the complex of immuno logical influences, operating during the development of the human fetus, were evaluated. it is obvious that during the early stages of pregnancy the conceptus is protected by non-specific mechanisms, i.e. hormonally (hcg, progesterone) and by certain properties of the trophoblast (barrier function, immunologically inert surface). specific immunological tolerance is formed by gradual penetration of trophoblast ... | 1988 | 3147589 |
| comparison of the invasion strategies used by salmonella cholerae-suis, shigella flexneri and yersinia enterocolitica to enter cultured animal cells: endosome acidification is not required for bacterial invasion or intracellular replication. | strains of escherichia, salmonella, shigella and yersinia actively enter eukaryotic cells. several techniques were used to compare and contrast the invasion mechanisms of salmonella cholerae-suis, yersinia enterocolitica and shigella flexneri. three animal cell lines (cho, hep-2 and mdck) were examined for susceptibility to bacterial entry by these strains. levels of intracellular bacteria varied widely between cell lines, but cho cells were the most susceptible to bacterial invasion, hep-2 inva ... | 1988 | 3147700 |
| [lysosomal enzyme activity in the nerve tissue of various species of animals after administration of a neurotoxic pesticide aphos]. | | 1988 | 3149613 |
| production of prostaglandins and hete-compounds by isolated-cultivated bovine aortic endothelial cells. | the main product of aa conversion by baec is pgi2. other pgs were synthesized by confluent cells only in traces with exception of a hht-like compound which has still to be characterized in more details. a significant release of hete was only detected when baec were destroyed. under these conditions about 1 pmole/10(5) cells 15- and 12 hete were released by baec. taken into account the amounts of hete produced by endothelial cells as well as the partially contradictory activities of 12- and 15-he ... | 1988 | 3150276 |
| anti-cancer therapy with valine-depleted amino acid imbalance solution. | for the purpose of developing amino acid imbalance solution applicable to cancer treatment, we prepared seven kinds of amino acid imbalance solutions based on a 10% balanced amino acid solution and investigated the anti-tumor effects of each solution. the administration of valine-depleted amino acid solution for 8 days at a daily dose of 53 ml/rat (79.5 kcal/rat) resulted in the most significant inhibitory effects on the growth of hepatoma (ah109a) and mammary tumors (mrmt-1), the rate being 82. ... | 1988 | 3150873 |
| urinary infection and staghorn calculus in the left kidney. | | 1988 | 3153046 |
| acute circulatory effects of doxorubicin in the conscious dog. | experimental work on the mechanisms of acute doxorubicin-induced cardiotoxicity may contribute to a better understanding of the clinical problem of cardiac failure after treatment with anthracycline derivatives. we studied aortic pressure and heart rate continuously for 1 h following a bolus injection of doxorubicin (1 mg/kg) in 7 dogs. in contrast with previous studies in intact animals, no anesthesia was used in order to eliminate possible interactions of doxorubicin with other drugs. one minu ... | 1986 | 3153244 |
| shiga toxin. | | 1986 | 3153582 |
| effects of acetyl strophanthidin on duration of atrial fibrillation in the neurally-intact and blockaded dog. | although the inotropic and dromotropic effects of cardiac glycosides in atrial fibrillation (af) are well recognized, their action on af itself is not clear. accordingly, to determine whether cardiac glycosides prolong af, the duration of electrically induced af, atrioventricular conduction, and left ventricular function were assessed for 30 minutes before and for 30 minutes following intravenous administration of acetyl strophanthidin (as), 20 micrograms/kg, in neurally intact, beta-blocked, an ... | 1988 | 3154634 |
| [a new serotype of shigella flexneri in czechoslovakia?]. | | 1985 | 3155665 |
| synthesis of new polyoxin derivatives and their activity against chitin synthase from candida albicans. | two analogues of l-alanylpolyoxin c with a modified peptide bond were synthesized and tested for inhibition of chitin synthase in candida albicans. n-methylation of the peptide bond (compound 13) or the replacement of it by nh2ch2 (compound 9) led to loss of activity in the enzyme assay. a novel analogue (compound 5) of nikkomycin was synthesized from uracil polyoxin c and (2s,3r)-3-hydroxyhomotyrosine, a component of echinocandin c. despite high activity in the chitin synthase assay, 5 had no i ... | 1985 | 3156247 |
| distribution of sensory receptors in the flexor carpi radialis muscle of the cat. | the structures and distribution of encapsulated muscle receptors were examined in serial transverse sections of flexor carpi radialis in the adult cat. four types of receptors (muscle spindles, golgi tendon organs, paciniform, and pacinian corpuscles) were identified. their structures resembled those encountered in other limb muscles. pacinian corpuscles were rare and occurred only in the external fascial coat of the muscle near its origin. the other three receptor types were distributed in an u ... | 1985 | 3156250 |
| expression of laminin receptor in normal and carcinomatous human tissues as defined by a monoclonal antibody. | it has been hypothesized that epithelial and endothelial cells interact with the laminin component of basement membranes via a cell surface laminin receptor molecule. it has also been proposed that the expression of this molecule may be involved in the invasion of carcinoma cells from their tissue of origin and their subsequent penetration through blood vessel basement membranes. we report here the use of a monoclonal antibody, lr-3, to define the expression of laminin receptor in normal, dyspla ... | 1985 | 3157447 |
| characterization of protein kinase c and its role in catecholamine secretion from bovine adrenal-medullary cells. | protein kinase c activity towards exogenous histone was detected in a cytosolic fraction of bovine adrenal medulla. the enzyme was dependent on ca2+ and phosphatidylserine for its activity, with half-maximal activation being achieved at approx. 18 microm free ca2+ and 8 micrograms of phosphatidylserine/ml. both diolein and 4 beta-phorbol 12-myristate 13-acetate (tpa) decreased the ca2+ requirement of the enzyme, half-maximal activation being obtained at approx. 12 microm and 9 microm free ca2+ r ... | 1985 | 3159383 |
| synthesis of aerobactin and a 76,000-dalton iron-regulated outer membrane protein by escherichia coli k-12-shigella flexneri hybrids and by enteroinvasive strains of escherichia coli. | one of the chromosomal segments associated with the virulence of shigella flexneri and transferred to escherichia coli k-12 by conjugation has been shown to code for the production of aerobactin and for the synthesis of an iron-regulated 76,000-dalton (76k) outer membrane protein. analysis of various e. coli k-12-s. flexneri transconjugants showed that the genes involved with the synthesis of aerobactin and with the production of the 76k protein were linked to the mtl region of the s. flexneri c ... | 1985 | 3159680 |
| metabolism of thymoxamine. i. studies with 14c-thymoxamine in rats. | thymoxamine is rapidly and completely absorbed in rats. it is a prodrug which does not enter the systemic circulation in its unchanged form. after either oral or intravenous administration it undergoes rapid and intense metabolism involving four biotransformation reactions: enzymatic hydrolysis to the corresponding phenol (metabolite i), monodemethylation to metabolite ii, sulfate conjugation of i and ii (metabolites iii and iv) and conjugation of i and ii with glucuronic acid (metabolites v and ... | 1985 | 3161738 |
| luteolytic potency of 16-phenoxy-derivatives of prostaglandin f2 alpha. | the binding of 16-phenoxy derivatives of prostaglandin (pg) f2 alpha to rat luteal membranes, and also their abortifacient potency in pregnant rats, have been studied. competitive binding studies with various pg-analogues were performed in ovaries of juvenile rats pretreated with pmsg and hcg, and in parallel studies the abortifacient potency of these substances was tested in pregnant rats. it was observed that this class of derivatives bound to the pgf2 alpha receptor as well as, or even better ... | 1988 | 3162421 |
| transforming growth factor-beta-induced growth inhibition and cellular hypertrophy in cultured vascular smooth muscle cells. | we have explored the hypothesis that hypertrophy of vascular smooth muscle cells may be regulated, in part, by growth inhibitory factors that alter the pattern of the growth response to serum mitogens by characterizing the effects of the potent growth inhibitor, transforming growth factor-beta (tgf-beta), on both hyperplastic and hypertrophic growth of cultured rat aortic smooth muscle cells. tgf-beta inhibited serum-induced proliferation of rat aortic smooth muscle cells (ed50 = 2 pm); this is ... | 1988 | 3166463 |
| a correlation between regional acetylcholinesterase activity in rat brain and performance in a spatial task. | the acquisition and retention of a water maze task was examined in 12 intact, young wistar rats. acetylcholinesterase activity in 43 discrete brain regions was then measured in the same rats by quantitative histochemistry. individual learning and retention indices were found to be significantly correlated with acetylcholinesterase (ache) levels in specific regions, e.g. cholinergic nuclei; the ventral pallidum and nucleus basalis; and in the dentate gyrus of the hippocampus. high levels of ache ... | 1988 | 3166718 |
| changes in vascular reactivity in experimental diabetic rats: comparison with hypothyroid rats. | the responsiveness to vasoactive agents in the perfused mesenteric vascular bed of streptozocin-induced diabetic rats was examined and compared with that of propylthiouracil-induced hypothyroid rats. diabetic rats at 4 and 8 weeks after the induction of diabetes showed a significant decrease in isoproterenol-induced vasodilatation. in addition, the contractile responses to norepinephrine and 5-hydroxytryptamine and the vasodilative response to acetylcholine were significantly decreased in 12-wee ... | 1988 | 3167224 |
| production of tumor necrosis factor in nude mice by muramyl peptides associated with bacterial vaccines. | gram-negative vaccines can elicit the production of tumor necrosis factor (tnf) in mice primed by muramyl dipeptide (mdp) or by its lipophilic derivative mdp-dipalmitoyl glycerol (mdp-gdp). in mice pretreated with mdp and particularly with mdp-gdp, bordetella pertussis vaccine was shown to be more effective than typhoid vaccine. the time course of tnf production in the blood did not indicate any difference between the effect of mdp or of mdp-gdp. in both cases the cytotoxic activity reached maxi ... | 1988 | 3167834 |
| production of human-human hybridomas secreting monoclonal antibodies reactive to breast cancer cell lines. | we produced human-human hybridomas by fusing lymph node lymphocytes of breast cancer patients with a fusion partner, ho-323 cells, in the presence of 50% polyethylene glycol, and screened hybridomas producing monoclonal antibody (moab) reactive to a breast cancer cell line, mcf-7. among 11 hybridomas secreting igm reactive to mcf-7, four hybridomas (h15a3, h15f2, k15b4, and k15c3) produced igm reacting specifically to breast cancer cell lines (mcf-7 and hbc-5). among them, h15a3 proliferated in ... | 1988 | 3170451 |
| effect of vagotomy on bile acid absorption by rat small intestine in vitro. | | 1988 | 3175250 |
| multiple cysteine proteinase forms during the life cycle of dictyostelium discoideum revealed by electrophoretic analysis. | proteinases of the cellular slime mould dictyostelium discoideum have been analysed using electrophoresis on polyacrylamide gels containing gelatin (gelatin/page). multiple proteinase forms were apparent in vegetative myxamoebae, but the presence of individual enzyme forms depended on the manner in which the cells were grown. axenic cells had a characteristic a-pattern of proteinases consisting of six bands, the most active enzymes having apparent mr values of 51,000 and 45,000 (these have been ... | 1988 | 3178750 |
| activity of glucosidase inhibitors against hiv infections. | | 1988 | 3182426 |
| heterogeneous colony forming ability in vitro of the abnormal clone-derived granulocyte-macrophage precursors in myelodysplastic syndromes. | the clonal origin of granulocyte-macrophage colony forming cells (cfu-gm) in the myelodysplastic syndrome (mds) was cytogenetically studied. chromosome analysis was carried out on single gm-colonies from six patients with mds, whose bone marrow cells had chromosome abnormalities. abnormal clone-derived cfu-gm were grown in four patients under the presence of human placental conditioned medium. in the remaining two, all analysed colonies revealed a normal karyotype, although the majority of metap ... | 1988 | 3184986 |
| measurement of local cerebral glucose utilization before and after v-p shunt in congenital hydrocephalus in rats. | local cerebral glucose utilization (lcgu) was measured by the [14c]deoxyglucose autoradiographic method in rats with congenital hydrocephalus before and after a v-p shunt. lcgu was decreased throughout the brain in hydrocephalus. the most severely affected regions were found in the cortex, with which a marked degree of subcortical cerebrospinal fluid (csf) edema was associated; regions in the brain-stem structures were less affected. these metabolic disturbances were improved in many regions aft ... | 1988 | 3185424 |
| thoracic cord compression in scheuermann's disease. | a 29-year-old man with spastic paraparesis of insidious onset was discovered to have thoracic cord compression by a combination of kyphosis and thoracic disc herniation. radiologic evaluation showed evidence of scheuermann's disease, of which thoracic kyphosis is one manifestation. neurologic signs are uncommon in scheuermann's disease and few cases have been successfully managed with resolution of neurologic deficit. this patient made a virtually complete recovery after thoracic cord decompress ... | 1988 | 3187711 |
| progressive ankylosis in mice. an animal model of spondylarthropathy. i. clinical and radiographic findings. | to determine its similarity to human spondylarthropathies, we studied murine progressive ankylosis, a spontaneously occurring disorder of joints in mice. clinically, peripheral joints were inflamed initially, then became ankylosed in a predictable sequence from distal to proximal. forefeet were involved before hindfeet. axial joint involvement produced severe spinal ankylosis. extraarticular manifestations included balanitis and crusting skin lesions. radiographically, bony erosions and calcific ... | 1988 | 3190783 |
| characterization of two deletions that remove the entire human zeta-alpha globin gene complex (- -thai and - -fil). | we have fully characterized two alpha thalassaemia mutants that occur in southeast asia, - -thai and - -fil. each mutant is due to a deletion that removes the entire zeta-alpha-globin gene complex. localization of the 5' breakpoints described here, allows the identification of unique fragments that are specific for each of the two mutations. this information can be used to assess the frequency of these mutants in southeast asia and will be of value in prenatal testing for alpha thalassaemia in t ... | 1988 | 3191033 |
| [effect of the induction of liver cytochrome p-450 by a chemically inert fluorocarbon on the reactions of immediate and delayed hypersensitivity]. | the effect of chemically inert perfluorodecalin (pfd) on the development of hypersensitive reactions of immediate and delayed types in guinea pigs and mice was studied. it was found that administration of pfd to animals both before sensitization (mice) and during the development of sensitization (guinea pigs) reduced the degree of the both types of hypersensitivity. | 1988 | 3191983 |
| mobile sequences in the pyruvate dehydrogenase complex, the e2 component, the catalytic domain and the 2-oxoglutarate dehydrogenase complex of azotobacter vinelandii, as detected by 600 mhz 1h-nmr spectroscopy. | 600 mhz 1h-nmr spectroscopy demonstrates that the pyruvate dehydrogenase complex of azotobacter vinelandii contains regions of the polypeptide chain with intramolecular mobility. this mobility is located in the e2 component and can probably be ascribed to alanine-proline-rich regions that link the lipoyl subdomains to each other as well as to the e1 and e3 binding domain. in the catalytic domain of e2, which is thought to form a compact, rigid core, also conformational flexibility is observed. i ... | 1988 | 3191993 |
| the disposition of [2,3-14c]-methyl and [2,3-14c]-2-ethylhexyl acrylate in male wistar albino rats. | the disposition of methyl [2,3-14c]-acrylate (ma) and 2-ethylhexyl [2,3-14c]-acrylate (eha) following intraperitoneal and oral administration to rats has been studied. the 14c found in the tissues was mainly associated with liver, kidneys and lungs. loss of 14c from these tissues occurred fairly rapidly, excluding the rats given eha intraperitoneally. most of the administered acrylates underwent rapid metabolism and excretion with expired air (more than 50% of the dose and urine (10-50% of the d ... | 1988 | 3196152 |
| dog owners and their use of veterinary services. | | 1988 | 3198454 |
| phaeohyphomycosis caused by alternaria alternata in a cat. | phaeohyphomycosis caused by alternaria alternata was diagnosed in a 6-year-old cat. a lesion in the nose resulted in enlargement of the dorsum of the nose. similar appearing lesions had been removed from the dorsum of the nose 1 and 4 years earlier. the lesion recurred 3 months after surgical excision and irregular administration of ketoconazole. a second cytoreductive operation followed by 5 months' treatment with ketoconazole resolved the infection. nasal trauma occurring at 8 months and at 5 ... | 1988 | 3198465 |
| a novel 190 kda calmodulin-binding protein associated with brain actomyosin. | we present evidence for a novel calmodulin-binding protein associated with ca2+-sensitive, non-muscle actomyosin from rabbit brain. gel filtration chromatography of brain actomyosin and sds-page analysis of resulting fractions revealed the presence of a stable complex of a 190 kda polypeptide and calmodulin. furthermore, brain actomyosin was retained on a column of conjugated calmodulin-sepharose 4b and eluted by 6 m urea in the presence of ca2+. further elution with 6m urea plus egta released t ... | 1988 | 3203157 |
| [ontogenetic characteristics of ascorbic acid binding by nerve tissue proteins]. | the highest ascorbic acid content has been found in a low-molecular fraction (m. w. less than 6000) of water-soluble proteins from brain tissue homogenates of rats, the lowest one--in a high-molecular fraction (m. w. about 65,000). the content of protein-bound ascorbic acid decreases during postnatal life. this process is accompanied by changes in the ratio of the reduced and oxidated forms of the ascorbic acid in favour of the latter. | 1988 | 3206951 |
| connective tissue remodelling in the ovine cervix during pregnancy and at term. | cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ... | 1988 | 3208512 |
| connective tissue remodelling in the ovine cervix during pregnancy and at term. | cervical ripening and dilation in the ewe has been quantitated in terms of the connective tissue content of the tissue and the biosynthesis of the macromolecular components which make up the extracellular matrix of this tissue. during pregnancy there is a marked increase in the total tissue mass of the cervix along with the total mass of hydroxyproline (collagen) and hexuronate (proteoglycans and hyaluronate). the concentration of hydroxyproline of the cervix based on wet weight of the tissue de ... | 1988 | 3208512 |
| paracrine control of immature sertoli cells by adult germ cells, in the rat (an in vitro study). cell-cell interactions within the testis. | enriched populations of germ cells prepared from adult rats were found to influence 20-day-old rat sertoli cell secretory activity by stimulating androgen-binding protein (abp) and inhibiting oestradiol-17 beta production in the presence of follicle-stimulating hormone (fsh) as well as of dibutyryl cyclic amp (dbcamp). among the different populations tested in coculture, pachytene spermatocytes were the most effective at stimulating abp and inhibiting oestradiol production, whereas early spermat ... | 1988 | 3208988 |
| gut-associated lymphoid tissue in the large intestine of calves. ii. electron microscopy. | scanning electron microscopy of lymphoid tissue in the large intestine of three germfree calves (age 3, 6, and 7 days) revealed two different units: propria nodules and lymphoglandular complexes (lgc). propria nodules had lymphoid tissue predominantly in lamina propria and were covered by distinct follicle-associated epithelium which lacked goblet cells; nodules were surrounded by wide crypts, which were also lined by follicle-associated epithelium towards the luminal side. lymphoglandular compl ... | 1988 | 3212895 |
| [facilitatory effect of trifluoperazine on adrenergic transmitter release from rat portal vein]. | | 1988 | 3218532 |
| arautp-affi-gel 10: a novel affinity absorbent for the specific purification of dna polymerase alpha-primase. | for the specific purification of eukaryotic dna-dependent dna polymerase alpha, we prepared two novel affinity resins bearing 5-(e)-(4-aminostyryl) arautp as a ligand. one of them was arautp-sepharose 4b which was coupled directly with the ligand and the other was arautp-affi-gel 10 which was coupled with the ligand through a spacer. no dna polymerase alpha-primase activity from cherry salmon (oncorhynchus masou) testes was bound on the arautp-sepharose 4b in all cases examined. on the other han ... | 1988 | 3218743 |
| affinity of microorganisms of the genus ureaplasma to the reproductive organs of cattle. | the purpose of this work was to define more precisely the role of ureaplasma organisms in the aetiology of granular vulvovaginitis and balanoposthitis (gvvbp) of cattle. to contribute to this question the frequency and degree of infection with ureaplasmas in two main groups of cattle was taken into account: (a) in cattle with symptoms of the mentioned disease, (b) in cattle without clinical symptoms. the samples of semen from 301 sires with symptoms of gvvbp and from 43 healthy sires as also vag ... | 1988 | 3219829 |
| epidemiology of hiv infection in africa. | hiv infection in africa is primarily acquired through heterosexual activity, accounting for up to 80% of cases. prostitutes and sexually promiscuous individuals are at particularly high risk of acquiring infection via this route. in the general population, women between the ages of 18 and 30 years are at increased risk of transmission. the role of cofactors, particularly concurrent sexually transmitted diseases (stds), appears to facilitate heterosexual spread. these groups represent opportuniti ... | 1988 | 3225742 |
| age and lpr dependent induction of increased sensitivity to the toxic effects of lipopolysaccharide and indomethacin in mrl mice: evidence for res activation during disease progression. | injection of 4.5-6 month old female mrl-lpr/lpr mice with 200-250 micrograms lipopolysaccharide led to the death of the animals within two days. treatment of age matched female control mice (mrl-+/+) or young (3 month) mrl-lpr/lpr mice did not lead to similar toxicity. however, pretreatment of young mice with the pyridine extract of residue of corynebacterium parvum (alternatively designated propionibacterium acnes) prior to lipopolysaccharide injection, led to the rapid death of 100% of the mic ... | 1988 | 3230580 |
| [the genetic activity of an alkaline solution of formaldehyde]. | toxicity and genetic activity of disinfectant consisting of 1.5% paraform and 0.5% naoh were studied. it was found that the preparation caused a weak mutagenic effect on crepis capillaris and chlorella roots, but had no effect in the ames test and gonadal cells of mammals. the studies of mutagenic effect of the preparation on somatic cells of white mice show limits of the cytogenetic effect at the level of 3.5 g/kg (1/10 ld50). | 1988 | 3232201 |
| individual free fatty acids and lactate uptake in the human heart during severe sepsis. | coronary haemodynamics and myocardial metabolism of nonesterified fatty acids (nefa) and lactate were studied in 11 patients with severe sepsis, and compared to 10 control subjects. coronary sinus blood flow was evaluated by thermodilution. arterial and coronary sinus blood samples were collected for the measurement of lactate and total and individual nefa concentrations both in septic and control patients. there was an increase in lactate and total nefa arterial concentrations with a marked inc ... | 1988 | 3232957 |
| an increase of collagen biosynthesis precedes other symptoms of ethanol-induced liver damage in rats. | treatment of rats with 10% ethanol for 6 months induced a 3-4-fold increase of [3h]proline incorporation into liver collagen and enhanced the level of prolyl hydroxylase activity in liver and serum of intoxicated rats. several diagnostic tests used to assess liver disease failed to demonstrate any symptom of liver damage. therefore, increased collagen biosynthesis appears to precede other symptoms of liver damage induced by ethanol. | 1988 | 3234225 |
| [infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients]. | | 1988 | 3247624 |
| [infection by the human immunodeficiency virus and the lymphadenopathy clinical picture. clinico-developmental study of 86 patients]. | | 1988 | 3247624 |
| [effect of the growth rate on the activity of nitrogen metabolism enzymes in chemostat cultures of candida diddensii and candida tropicalis]. | | 1988 | 3247869 |
| effects of an extract of feverfew (tanacetum parthenium) on arachidonic acid metabolism in human blood platelets. | it has been suggested that feverfew extracts (fe) inhibit platelet behaviour via effects on platelet sulphydryl (sh) groups. in the present study we found evidence that fe inhibits uptake as well as liberation of arachidonic acid (aa) into/from platelet membrane phospholipids (pl). | 1988 | 3248111 |
| depression of intestinal epithelial cell production rate by hindgut bypass in rats. | influence of hindgut bypass on the epithelial cell production rates of the small and large intestine was tested in rats. hindgut bypass decreased cryptal cell production rates of the jejunum, ileum, cecum, and distal colon by 40-65%. these results suggest that the entrance of ileal effluent into the hindgut and, presumably, the resultant microbial production of short-chain fatty acids are required to maintain the normal epithelial cell proliferation not only in the hindgut but also in the small ... | 1988 | 3249918 |
| a model for simultaneous study of pressure and electric events in the rabbit sphincter of oddi and duodenum. | an animal experimental model featuring simultaneous recordings of electromyography and pressure from the sphincter of oddi (so) and duodenum is demonstrated. on the basis of data from 10 rabbits, pressure recordings from the so were shown to have phasic activity with an average basic pressure peak interval of 2.6 to 3.5 sec. histographic analysis of so pressure recordings showed a multimodal appearance, suggesting that the activity is paced. a substantial amount of overlap between so and duodena ... | 1988 | 3249919 |
| significance of heel pad confinement for the shock absorption at heel strike. | shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ... | 1988 | 3253241 |
| significance of heel pad confinement for the shock absorption at heel strike. | shock absorption (sa) is a simple way to reduce the body load and can be used in the prevention and treatment of injuries. the heel pad is the most important shock absorber in the shoe heel complex. the purpose of this study was to investigate whether the sa at heel strike can be increased by heel support in people and shoes with high or low sa. the impact forces at heel strike were measured on an amti (r) force platform. fourteen legs were tested in seven persons (nine with normal and five with ... | 1988 | 3253241 |
| phagocytic and bactericidal properties of macrophages of mice immunized with outer membrane proteins (omp) of shigella. | macrophages obtained from peritoneal exudates of mice immunized with the single doses (1 and 5 micrograms) of omp were shown to have stronger phagocytic as well as bactericidal properties in relation to shigella flexneri bacilli than nonactivated macrophages. macrophages from the animals immunized with 10, 20 and 30 micrograms omp doses showed phagocytic and bactericidal properties similar to those of nonactivated macrophages while the immunization with the dose 50 micrograms resulted in their s ... | 1988 | 3254119 |
| the role of serum factors and influence of intravenous immunoglobulins on phagocytosis of campylobacter species. | | 1988 | 3255396 |
| the relationship between blood and fertility parameters in post partum dairy cows. | | 1988 | 3256237 |
| the relationship between blood and fertility parameters in post partum dairy cows. | | 1988 | 3256237 |
| the effects of anions on fluid reabsorption from the proximal convoluted tubule of the rat. | 1. fluid reabsorption from surface proximal tubules of the rat was measured in vivo using stationary microperfusion techniques. reabsorptive rate (jv) was measured from droplets containing chloride as the main reabsorbable anion and when chloride was substituted by bromide, iodide, nitrate, acetate, isethionate or methylsulphate in either the tubular lumen alone or in both lumen and peritubular capillaries. 2. in tubules with an intact blood supply, droplet volume decreased in a manner best desc ... | 1988 | 3256612 |
| reticuloendothelial clearance and splenic mononuclear cell populations after resection and autotransplantation. | although the preservation of splenic tissue may prevent overwhelming infection after splenectomy, the degree of protection conferred by small remnants has not been optimal. we investigated whether either splenic reticuloendothelial clearance of a blood flow-dependent colloid or macrophage and t-cell populations might be altered by resection or autotransplantation of the spleen. our results have shown that bloodstream reticuloendothelial clearance of technetium 99m sulfur colloid is not impaired ... | 1988 | 3257658 |
| hydrogen peroxide-mediated inhibition of t-cell response to mitogens is a result of direct action on t cells. | hydrogen peroxide, a reactive oxygen intermediate produced by activated neutrophils, has been shown to inhibit the response of human t lymphocytes to mitogens and alloantigens. since hydrogen peroxide is known to react with iron and to induce lipid peroxidation, we compared the effects of hydrogen peroxide and a lipid peroxidation product, malondialdehyde, on the response of human peripheral blood mononuclear cells to t-cell mitogens. peripheral blood mononuclear cells pretreated with 1 mmol/l o ... | 1988 | 3257684 |
| lymphocyte chimerism in a full allogeneic composite tissue (rat-limb) allograft model prolonged with cyclosporine. | lew recipients of aci vascularized hind limb allografts were analyzed for lymphoid chimerism by a complement-dependent cytotoxicity assay using antisera produced across this strain combination. in assessing the technique, two lew recipients of sublethal irradiation (400 rad), aci bone-marrow allografts, and csa exhibited mixed lymphoid chimerism 23 days posttransplant. short-term csa-treated cta recipients that were assayed at various times following transplantation and underwent subacute reject ... | 1988 | 3259042 |
| human cryptosporidiosis in north queensland. | during a 12-month period, feces from 780 persons from the townsville region were evaluated by the kinyoun acid-fast strain, and 36 (4.6%) immunocompetent patients were found to have cryptosporidium oocysts. twenty-five index cases were identified; 13 (8.6%) cases from 151 patients were from palm island, an isolated aboriginal community in the wet tropics and 12 (1.9%) cases from 629 patients were from the dry tropics of townsville. all 11 secondary cases were associated with a person-to-person o ... | 1988 | 3264149 |
| electrophysiologic effects of aspirin on myocardium: interaction with catecholamine. | | 1988 | 3269682 |
| electrophysiologic effects of aspirin on myocardium: interaction with catecholamine. | | 1988 | 3269682 |
| contamination of freshly slaughtered pig carcasses with human pathogenic yersinia enterocolitica. | evidence is presented for the extent of contamination of freshly slaughtered pig carscasses with human pathogenic yersinia enterocolitica and shows the significance of faecal contamination as a source of infection. swab samples collected from the rectum and the surface of a total of 1458 pig carcasses were examined for the presence of human pathogenic y. enterocolitica. y. enterocolitica, biovar iv, serogroup 0:3, were isolated from the rectum of 360 pigs (24.7%). the organism was isolated from ... | 1988 | 3275322 |
| experimental portal fibrosis produced by intraportal injection of killed nonpathogenic escherichia coli in rabbits. | an attempt was made to develop an animal model for the study of the etiology of noncirrhotic portal fibrosis or idiopathic portal hypertension based on the assumption that it is related to chronic abdominal infection. rabbits were given killed nonpathogenic escherichia coli intraportally or intravenously. the animals to which a mixture of killed e. coli and rabbit antiserum (aggregated e. coli) was given intraportally developed remarkable histologic changes in the liver. the early inflammatory r ... | 1988 | 3276575 |
| shigella bacteremia. | | 1988 | 3277577 |
| a basic replicon of virulence-associated plasmids of shigella spp. and enteroinvasive escherichia coli is homologous with a basic replicon in plasmids of incf groups. | shigella species and enteroinvasive escherichia coli strains carry a large (120- to 140-megadalton) plasmid called pinv, which contains genes essential for the invasiveness of these pathogens. hybridization with specific probes derived from the repfic and repfib replicons of the incf1 ent plasmid p307 showed that pinvs present in 35 clinical isolates are homologous with repfic but not repfib, regardless of the serogroup of the shigella or e. coli strain. repfic of p307, in turn, is very similar ... | 1988 | 3278983 |
| combined parenteral and oral immunization results in an enhanced mucosal immunoglobulin a response to shigella flexneri. | achieving a vigorous secretory immunoglobulin a (iga) response in intestinal secretions usually requires multiple doses of antigen given orally, while systemic immunity is more easily attained by parenteral immunization. this study examines the role of combined parenteral and oral immunizations to enhance the early mucosal immune response to an enteropathogen. we have used a chronically isolated intestinal-loop model in rabbits as a probe to monitor kinetically the initial (primary) local immune ... | 1988 | 3278985 |
| protein binding sites on escherichia coli 16s ribosomal rna; rna regions that are protected by proteins s7, s9 and s19, and by proteins s8, s15 and s17. | selected groups of isolated 14c-labelled proteins from e. coli 30s ribosomal subunits were reconstituted with 32p-labelled 16s rna, and the reconstituted complexes were partially digested with ribonuclease a. rna fragments protected by the proteins were separated by gel electrophoresis and subjected to sequence analysis. complexes containing proteins s7 and s19 protected an rna region comprising helices 29 to 32, part of helix 41, and helices 42 and 43 of the 16s rna secondary structure. additio ... | 1988 | 3279390 |
| toxoplasmosis and the acquired immune deficiency syndrome. | the literature on toxoplasmosis in the acquired immune deficiency syndrome is reviewed with reference to more than 140 cases from various centres. the incidence, pathogenesis, clinical features, diagnosis, treatment and prognosis are considered. | 1988 | 3280690 |
| quality of commercially produced shigella serogrouping and serotyping antisera. | shigella grouping antisera from five manufacturers and typing antisera from two were purchased and evaluated with homologous and heterologous shigella strains in the slide agglutination test. only 31 of 73 (42%) antisera were satisfactory. in many instances, the antisera gave negative, as opposed to weak, reactions when they should have given strong positive reactions. four reagents cross-reacted with shigella strains. of the 19 polyvalent grouping antisera to subgroups shigella dysenteriae sero ... | 1988 | 3281972 |
| cardiac nuclear medicine, present status, future hopes as seen from the view of a cardiologist. | the present techniques of nuclear medicine that are used in cardiology are critically evaluated. they are used mainly for the assessment of coronary circulation (measurement of regional flow and perfusion), on the determination of left ventricular muscle function (radionuclear ventriculography) and on the study of cardiac metabolism. the last-mentioned technique is unique insofar as (except for magnetic resonance imaging) there is no other method available to analyse myocardial metabolism noninv ... | 1988 | 3282883 |