| a self-loading microfluidic device for determining the minimum inhibitory concentration of antibiotics. | this article describes a portable microfluidic technology for determining the minimum inhibitory concentration (mic) of antibiotics against bacteria. the microfluidic platform consists of a set of chambers molded in poly(dimethylsiloxane) (pdms) that are preloaded with antibiotic, dried, and reversibly sealed to a second layer of pdms containing channels that connect the chambers. the assembled device is degassed via vacuum prior to its use, and the absorption of gas by pdms provides the mechani ... | 2011 | 22193301 |
| a threat of the klebsiella pneumoniae carbapenemase-producing strains among transplant recipients. | infections due to klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae are associated with increased therapeutic failure and mortality. our laboratory recognized several strains producing kpc, most of which originated from transplantation ward patients. | 2011 | 21996247 |
| identification of the methyl phosphate substituent at the non-reducing terminal mannose residue of the o-specific polysaccharides of klebsiella pneumoniae o3, hafnia alvei pcm 1223 and escherichia coli o9/o9a lps. | o-specific polysaccharides of gram-negative bacteria are synthesized by two different mechanisms: polymerization of the pre-formed o-repeating unit or sequential addition of the monosaccharides to the growing polysaccharide chain. in the second case, growth of the polymer can be further subdivided into two groups depending on the presence or absence of a special monosaccharide or non-sugar substituent that terminates the glycan. a family of polymannose o-polysaccharides provides prototypes for t ... | 2011 | 22169179 |
| pneumonia due to pandemic (h1n1) 2009 influenza virus and klebsiella pneumoniae capsular serotype k16 in a patient with nasopharyngeal cancer. | streptococcus pneumoniae, haemophilus influenzae, staphylococcus aureus and group a streptoccocus, but no klebsiella pneumoniae were responsible for bacterial coinfections during the 2009 and previous influenza pandemics. we hereby report a case with concurrent bacteremic pneumonia due to an unusual capsular serotype k16 k. pneumoniae and pandemic (h1n1) 2009 influenza in a patient with nasopharyngeal cancer. such a coinfection has not previously been described. | 2011 | 22153762 |
| Antimicrobial activities of three species of family mimosaceae. | The antimicrobial activities of crude methanolic extract of leaves of Acacia nilotica L., Albizia lebbeck L. and Mimosa himalayana Gamble belonging to family mimosaceae were investigated in this research work. Antibacterial activity was studied by agar well diffusion method against one gram-positive Bacillus subtilis and three gram-negative Pseudomonas aeruginosa, Escherichia coli and Klebsiella pneumonia. Crude extract of all plants showed best activity against gram-negative bacterial strains w ... | 2012 | 22186331 |
| Emergence in Japan of an imipenem-susceptible, meropenem-resistant Klebsiella pneumoniae carrying bla(IMP-6). | We identified 5 Klebsiella pneumoniae isolates showing high resistance to ß-lactams except imipenem and designated them ISMRK (imipenem-susceptible but meropenem-resistant Klebsiella). They carried the bla(IMP-6) and bla(CTX-M-2) on a self-transmissible plasmid. ISMRK may be falsely categorized as susceptible to carbapenems if imipenem is used to screen carbapenem resistance. | 2012 | 22078906 |
| identification of an antimicrobial peptide from human methionine sulfoxide reductase b3. | human methionine sulfoxide reductase b3a (hmsrb3a) is an endoplasmic reticulum (er) reductase that catalyzes the stereospecific reduction of methionine-r-sulfoxide to methionine in proteins. in this work, we identified an antimicrobial peptide from hmsrb3a protein. the n-terminal er-targeting signal peptide (amino acids 1-31) conferred an antimicrobial effect in escherichia coli cells. sequence and structural analyses showed that the overall positively charged er signal peptide had an argand pro ... | 2011 | 22027001 |
| sensitive and specific modified hodge test for kpc and metallo-beta- lactamase detection in pseudomonas aeruginosa by use of a novel indicator strain, klebsiella pneumoniae atcc 700603. | we evaluated the ability of the modified hodge test to discriminate between kpc- and metallo-beta-lactamase (mbl)-producing pseudomonas aeruginosa isolates and carbapenemase nonproducers. with escherichia coli atcc 25922 as the indicator strain, the mht resulted in low sensitivity, specificity, and repeatability. replacing the indicator strain with klebsiella pneumoniae atcc 700603 led to an improved performance (100%, 97%, 0%, and 100% sensitivity, specificity, indeterminate results and repeata ... | 2011 | 22012019 |
| recurring klebsiella pneumoniae pyogenic liver abscesses in a resident of san diego, california, due to a k1 strain carrying the virulence plasmid. | we report a diabetic patient who had three episodes of cryptogenic liver abscess due to klebsiella pneumoniae. he was a caucasian who lived in california and had no epidemiological connection to east asia. the isolate from his third episode was a hyperviscous k1 strain that carried the klebsiella virulence plasmid. | 2011 | 21998428 |
| changing trends in mastitis. | abstract: the global dairy industry, the predominant pathogens causing mastitis, our understanding of mastitis pathogens and the host response to intramammary infection are changing rapidly. this paper aims to discuss changes in each of these aspects. globalisation, energy demands, human population growth and climate change all affect the dairy industry. in many western countries, control programs for contagious mastitis have been in place for decades, resulting in a decrease in occurrence of st ... | 2009 | 22082032 |
| the disinfecting potential of contact lens soutions used by sultan qaboos university students. | this study aimed to determine the disinfecting potential of some contact lens solutions used by some university students in oman. | 2011 | 21969898 |
| virulence of a klebsiella pneumoniae strain carrying the new delhi metallo-beta-lactamase-1 (ndm-1). | the aim of the study was to compare and evaluate virulence in five strains of klebsiella pneumoniae, including an isolate carrying new delhi metallo-beta-lactamase-1 (ndm-1). in vivo virulence was assessed using a murine sepsis model and using the nematode caenorhabditis elegans killing model, and in vitro virulence by assessing various virulence factors. the ndm-1 carrying k. pneumoniae isolate was the most virulent in the murine sepsis model but there was no clear cut correlation to in vitro v ... | 2011 | 21925284 |
| expression of esbl-like activity in infrequently encountered members of the family enterobacteriaceae. | a collection of 94 unusual members of the enterobacteriaceae were screened for the presence of extended spectrum β-lactamases (esbls) using the microscan esβl plus dried confirmation panel. presumptively positive strains were then confirmed for the presence of an esbl by double disk diffusion, e-test strips (ab biodisk, solna, sweden) and pcr for shv, tem, and ctx-m2 genes. of the 18 strains initially positive on the esβl panel only three strains (leminorella grimontii, klebsiella ozaenae, and k ... | 2011 | 22139464 |
| changes in the clinical features and prognostic factors of endogenous endophthalmitis: fifteen years of clinical experience in korea. | purpose:: to identify changes in the clinical features, management, and prognostic factors of endogenous endophthalmitis in korea from 1996 through 2010. methods:: we conducted a retrospective review of medical records of patients diagnosed with endogenous endophthalmitis from january 1996 to december 2010, which was divided into 3 periods: 1996 to 2000, 2001 to 2005, and 2006 to 2010. variables included age, source of infection, systemic conditions, initial and final visual acuities, causative ... | 2011 | 22105504 |
| antibiotic susceptibility and genotype patterns of escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa isolated from patients with urinary tract infection. | | 2011 | 22184937 |
| [susceptibility of klebsiella pneumoniae isolated from urinary tract infections to quinolones]. | urinary tract infections (utis) are the most prevalent infections worldwide both in males and females. k. pneumoniae is one of the major pathogens causing utis. fluoroquinolones are effective drugs for treating infections caused by klebsiella spp. the aim of this study was to evaluated the susceptibility to three fluoroquinolones of k. pneumoniae strains isolated from urine. the mics of ciprofloxacin was determined by agar dilution method and the mics of norfloxacin and gatifloxacin by e-test. a ... | 2011 | 22184897 |
| [severe primary liver abscess and septic pulmonary embolism due to klebsiella pneumoniae with hypermucoviscosity phenotype]. | a 70-year-old man with diabetes mellitus seen for fever, right chest pain, and right-lung field consolidation on chest x-ray was found in thoracoabdominal computed tomography (ct) to have variable-sized nodules in both lung fields and multiple low-density hepatic areas. on physical examination, his pulse was 145 beats per minute and blood pressure 92/68mmhg, indicating a preshock state. laboratory tests showed elevated wbc of 15,200/microl, serum-c-reactive protein (crp) of 34.4 mg/dl, and a dec ... | 2011 | 21861440 |
| regulatory role for l: -arginine in the utilization of amino acids by pig small-intestinal bacteria. | we recently reported that bacteria from the pig small intestine rapidly utilize and metabolize amino acids (aa). this study investigated the effect of l: -arginine on the utilization of aa by pure bacterial strains (streptococcus sp., escherichia coli and klebsiella sp.) and mixed bacterial cultures derived from the pig small intestine. bacteria were incubated at 37°c for 3 h in anaerobic aa media containing 0-5 mmol/l of arginine to determine the effect of arginine on the bacterial utilization ... | 2011 | 21928075 |
| response changes as gram neg hais rise. | | 2011 | 22039619 |
| chemical composition and antimicrobial activity of the essential oil of anthemis altissima l. var. altissima. | the essential oil obtained from the flowering parts of anthemis altissima l. var. altissima was analysed by gas chromatography and gas chromatography mass spectroscopy. in this study, 34 compounds representing 98.76% of the essential oil were identified. the main components were α-terpineol (26.42%), β-pinene (9.23%), cis-chrysanthenyl acetate (6.30%), globulol (5.36%), n-tricosane (4.41%), terpinen-4-ol (4.08%) and 1,8 cineole (3.84%). antibacterial activities of the essential oil and its two m ... | 2011 | 22007714 |
| the role of p-glycoprotein and breast cancer resistance protein (bcrp) in bacterial attachment to human gastrointestinal cells. | active efflux proteins such as p-glycoprotein (p-gp) are thought to have a protective role in the intestinal tract by preventing xenotoxin absorption. some bacteria also need to adhere to the intestinal tract before causing disease through adhesin secretion. thus, this study was initiated to examine whether any association exists between bacterial adhesion. | 2011 | 22115371 |
| metal complexes of diisopropylthiourea: synthesis, characterization and antibacterial studies. | co(ii), cu(ii), zn(ii) and fe(iii) complexes of diisopropylthiourea have been synthesized and characterized by elemental analyses, molar conductivity, magnetic susceptibility, ftir and electronic spectroscopy. the compounds are non-electrolytes in solution and spectroscopic data of the complexes are consistent with 4-coordinate geometry for the metal(ii) complexes and six coordinate octahedral for fe(iii) complex. the complexes were screened for their antibacterial activities against six bacteri ... | 2011 | 22072941 |
| microbiology of lower respiratory tract infections in benin city, nigeria. | lower respiratory tract infections are among the most common infectious diseases of humans worldwide and continue to be a major cause of morbidity in nigeria. this study focused on determining the microbial agents of lower respiratory tract infections, the effect of age and gender on its prevalence, and the susceptibility profile of bacterial isolates. | 2011 | 22135583 |
| role of argp (icia) in lysine-mediated repression in escherichia coli. | initially identified as an inhibitor of oric-initiated dna replication in vitro, the argp or icia protein of escherichia coli has subsequently been described as a nucleoid-associated protein and also as a transcriptional regulator of genes involved in dna replication (dnaa and nrda) and amino acid metabolism (argo, dapb, and gdha [the last in klebsiella pneumoniae]). argp mediates lysine (lys) repression of argo, dapb, and gdha in vivo, for which two alternative mechanisms have been identified: ... | 2011 | 21890697 |
| bile culture and susceptibility testing of malignant biliary obstruction via ptbd. | purpose: to assess the information obtained by bile culture and susceptibility testing for malignant biliary obstruction by a retrospective one-center study. methods: a total of 694 patients with malignant biliary obstruction received percutaneous transhepatic biliary drainage during the period july 2003 to september 2010, and subsequently, bile specimens were collected during the procedure. among the 694 patients, 485 were men and 209 were women, ranging in age from 38 to 78 years (mean age 62 ... | 2011 | 21904809 |
| Thermal treatment of the bacteriophage lysate of Klebsiella pneumoniae B5055 as a step for the purification of capsular depolymerase enzyme. | The lytic bacteriophage which produces the hydrolase enzyme capable of depolymerizing exopolysaccharide of Klebsiella pneumoniae and of other bacteria was isolated earlier. In this study, a simple method of depolymerase purification from the phage lysate by dissociating the enzyme from the phage particle was developed. The bacteriophage showed a relatively smaller plaque size surrounded by a wide halo indicating a depolymerase action on the capsular polysaccharide of K. pneumoniae B5055. The dep ... | 2012 | 22036659 |
| identification of new delhi metallo-β-lactamase gene (ndm-1) from a clinical isolate of acinetobacter junii in china. | new delhi metallo-β-lactamase-1 (ndm-1) is a novel type of metallo-β-lactamase (mbl) responsible for bacterial resistance to β-lactam antibiotics. acinetobacter junii was previously shown to possess a mbl phenotype; however, the genes responsible for this phenotype were not identified. in this study, we reported the identification of ndm-1 gene in a clinical isolate of a. junii from a child patient in china, which was resistant to all β-lactams except aztreonam but sensitive to aminoglycosides ... | 2011 | 22181044 |
| respiratory bacterial flora from healthy as well as respiratory symptoms' subjects. | respiratory system is the primary settlement place of opportunistic organisms and considered as chief carrier of common respiratory pathogens. the aim of the study was to know the opportunistic organisms present in the healthy subjects as well as subjects that were suffering from respiratory symptoms. the organisms were identified as per standard bacteriological protocol and pathogenicity tests of the identified organisms were performed in mouse model. antibiotic sensitivity of the identified or ... | 2011 | 21902058 |
| poor hospital hygiene is blamed for deaths of three babies in bremen. | | 2011 | 22089749 |
| expanding the clinical and genetic spectrum of human cd40l deficiency: the occurrence of paracoccidioidomycosis and other unusual infections in brazilian patients. | cd40 ligand (cd40l) deficiency or x-linked hyper-igm syndrome (x-higm) is a well-described primary immunodeficiency in which pneumocystis jiroveci pneumonia is a common clinical feature. we have identified an unusual high incidence of fungal infections and other not yet described infections in a cohort of 11 x-higm patients from nine unrelated brazilian families. among these, we describe the first case of paracoccidioidomycosis (pcm) in x-higm. the molecular genetic analysis of cd40l was perform ... | 2011 | 22193914 |
| development of immunization trials against klebsiella pneumoniae. | klebsiella pneumoniae is the most common cause of nosocomial respiratory tract and premature intensive care infections, and the second most frequent cause of gram-negative bacteraemia and urinary tract infections. drug resistant isolates remain an important hospital-acquired bacterial pathogen, add significantly to hospital stays, and are especially problematic in high impact medical areas such as intensive care units. many investigations worldwide proved the increasing resistance of such pathog ... | 2011 | 22100884 |
| biofouling characteristics and identification of preponderant bacteria at different nutrient levels in batch tests of a recirculating cooling water system. | understanding the influence of nutrient levels on biofouling control is an important requirement for management strategies in a recirculating cooling water system. nutrient limitation may be one way to control biofouling development without increasing biocide dosing. therefore, this study was carried out to investigate the effects of nutrient levels on biofouling characteristics and to identify the preponderant bacteria in the batch tests with a simulated cooling water system. the biofouling cha ... | 2011 | 21879565 |
| coexistence of bla(oxa-48) and aac(6')-ib-cr genes in klebsiella pneumoniae isolates from istanbul, turkey. | this study evaluated the presence of carbapenem hydrolysing β-lactamase genes and plasmid-mediated quinolone resistance (pmqr) determinants in 22 klebsiella pneumoniae isolates collected from the istanbul medical faculty, turkey, which reduced the susceptibility or resistance to carbapenem. the vitek(®) 2 system and e-tests were used to determine the minimum inhibitory concentrations needed to inhibit bacterial growth. genes were screened by polymerase chain reaction, and gene transferability w ... | 2011 | 22117996 |
| preparation and antimicrobial activity of poly (vinyl chloride)/gelatin/montmorillonite biocomposite films. | the aim of this study was using a novel antimicrobial thermoplastic plasticizer based on aliphatic anhydride derivative dodecenyl succinic anhydride (dsa) for blending poly (vinyl chloride), pvc, with gelatin in presence of montmorillonite (mmt) using brabender via polymer melting technique. this anhydride-based plasticizer blended the membrane ingredients homogenously under melting process. the used plasticizer exhibited high performance antimicrobial potency for some biomedical and industrial ... | 2011 | 21909641 |
| lemierre syndrome complicating multiple brain abscesses caused by extended-spectrum β-lactamase-producing klebsiella pneumoniae cured by fosfomycin and meropenem combination therapy. | a woman aged 56 years of age had a community-acquired left neck abscess and internal jugular vein thrombosis with septicemia due to extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae. even though she was treated with intravenous meropenem, the bacteremia persisted. she was complicated with multiple brain abscesses, seizure, and leucopenia. after a combination of intravenous fosfomycin and meropenem, her clinical condition became stable. combination treatment was continued for 2 ... | 2011 | 22154996 |
| [repression of bacterial biofilms in suppurative necrotic complications of diabetic foot syndrome by ultrasound cavitation]. | study of wound microbial landscape in suppurative necrotic complications in patients with diabetic foot syndrome (dfs), and a possibility of suppression of bacterial biofilm development in these wounds by ultrasound cavitation (usc). | 2011 | 21913385 |
| testing for cemo, klebsiella pneumoniae and pseudomonas aeruginosa. | | 2011 | 21949203 |
| diversity of extended-spectrum ?-lactamase-producing enterobacteriaceae on hospital admission. | abstract extended-spectrum ?-lactamase (esbl)-producing enterobacteriaceae have become prevalent in both the hospital and the community. we describe the epidemiology of esbl-producing isolates and patient characteristics at hospital admission. data on clinical properties, medical history, previous hospitalizations, and previous antibiotic treatments were collected. esbl genes (bla(ctx-m), bla(tem), and bla(shv)) were identified by polymerase chain reaction. one hundred and sixteen patients car ... | 2011 | 22066775 |
| risk factors for developing clinical infection with carbapenem-resistant klebsiella pneumoniae in hospital patients initially only colonized with carbapenem-resistant k pneumoniae. | background: this study examined predictors of carbapenem-resistant klebsiella pneumoniae (crkp) colonization and risk factors for the development of crkp infection in patients initially only colonized with crkp. methods: a total of 464 patients with crkp rectal colonization (crkp-rc) were identified. two case-control studies were performed, one comparing risk factors for crkp-rc in patients who did not develop crkp infection (crkp-in) versus patients without crkp-rc and crkp-in, and the other co ... | 2011 | 21906844 |
| antibacterial activity of optically transparent nanocomposite films based on chitosan or its derivatives and silver nanoparticles. | colloidal silver nanoparticles (nps) were prepared using the citrate and borohydride reduction methods and were then investigated as fillers in three matrices: unmodified chitosan, water-soluble chitosan and a n-alkyl chitosan derivative. the nanocomposites were used to prepare cast thin films (9-19μm thickness) and characterized for their optical and antimicrobial properties. the optical properties of the materials were adjusted either by varying the ag nps content in the films (0.5-3.9% w/w) o ... | 2011 | 22154478 |
| synthesis and biological activity of ethyl 2-(substituted benzylthio)-4-(3'-(ethoxycarbonyl)biphenyl-4-yl)-6-methyl-1,4-dihydropyrimidine-5-carboxylate derivatives. | in the present study, a new series of ethyl 2-(substituted benzylthio)-4-(3'-(ethoxycarbonyl)-biphenyl-4-yl)-6-methyl-1,4-dihydropyrimidine-5-carboxylate derivatives was synthesized. the newly synthesized compounds were characterized by (1) h-nmr, mass and c, h, n analyses. all newly synthesized compounds were screened for their antibacterial (escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, streptococcus pyogenes and klebsiella pneumoniae) and antifungal (aspergillus flavus, asp ... | 2011 | 21987259 |
| ancient antimicrobial peptides kill antibiotic-resistant pathogens: australian mammals provide new options. | to overcome the increasing resistance of pathogens to existing antibiotics the 10×'20 initiative declared the urgent need for a global commitment to develop 10 new antimicrobial drugs by the year 2020. naturally occurring animal antibiotics are an obvious place to start. the recently sequenced genomes of mammals that are divergent from human and mouse, including the tammar wallaby and the platypus, provide an opportunity to discover novel antimicrobials. marsupials and monotremes are ideal poten ... | 2011 | 21912615 |
| structure of new delhi metallo-β-lactamase 1 (ndm-1). | antibiotic resistance in bacterial pathogens poses a serious threat to human health and the metallo-β-lactamase (mbl) enzymes are responsible for much of this resistance. the recently identified new delhi mbl 1 (ndm-1) is a novel member of this family that is capable of hydrolysing a wide variety of clinically important antibiotics. here, the crystal structure of ndm-1 from klebsiella pneumoniae is reported and its structure and active site are discussed in the context of other recently deposite ... | 2011 | 22102018 |
| veterinary hospital-acquired infections in pets with a ciprofloxacin-resistant ctx-m-15-producing klebsiella pneumoniae st15 clone. | | 2011 | 22178643 |
| extended-spectrum β-lactamase producing klebsiella pneumoniae from blood cultures in puducherry, india. | | 2011 | 21985825 |
| mathematical modelling of microbial growth in ground beef from argentina. effect of lactic acid addition, temperature and packaging film. | the effects of: (i) storage temperature (0, 4 and 10°c), (ii) gaseous permeability of the packaging film (polyethylene and eva saran eva for vacuum packaging), and (iii) natural beef ph (5.6, 5.8 and 6.1) on the growth of different bacteria isolated from beef muscle were examined. the bacteria were klebsiella, pseudomonas sp. and escherichia coli. microbial growth was modelled using gompertz and linear equations. the effects of temperature on microbial growth rate (µ) and on lag phase duration w ... | 2008 | 22062911 |
| antimicrobial susceptibility to parenteral and oral agents in a largely polyclonal collection of ctx-m-14 and ctx-m-15-producing escherichia coli and klebsiella pneumoniae. | activity of oral and parenteral antimicrobials against consecutively isolated extended-spectrum β-lactamase (esbl)-producing escherichia coli (n = 149) and klebsiella pneumoniae (n = 20) was determined, and susceptibility test methods were compared for parenteral β-lactams. polymerase chain reaction (pcr) targeting bla(ctx-m), bla(shv) and bla(tem), and dna sequencing and epidemiological typing with pulsed-field gel electrophoresis were performed. pcr targeting pabb was screened for e. coli o25b ... | 2011 | 22085361 |
| clinical and bacteriological profile of urinary tract infection in children at nepal medical college teaching hospital. | the aim of this study was to study the clinical and bacteriological profile of urinary tract infection (uti) in children. this was a prospective study of 40 culture positive cases of uti in children who attended pediatric outpatient department of nepal medical college teaching hospital. uti was more common in female (65.0%) than in male (35.0%). half were in the age group 1- 5 years. fever was the most common presentation (65.0%) followed by abdominal pain (42.5%), decreased appetite (37.5%) and ... | 2011 | 21991696 |
| bacteriuria among adult non-pregnant women attending mulago hospital assessment centre in uganda. | urinary tract infections (utis) in women are a common problem in primary health care settings. resistance of bacterial uropathogens to commonly used antibiotics is common in many places. | 2011 | 21857848 |
| risk of bacterial colonization of pancreatic stents used in endoscopic retrograde cholangiopancreatography. | background:: pancreatic stents (pss) are commonly inserted at the time of endoscopic retrograde cholangiopancreatography to reduce the risk of pancreatitis. if left in situ for more than 2 weeks, they have been associated with pancreatic duct injury. the mechanism of this injury is not clear, but it may be related to bacterial colonization. aim:: to determine the incidence of ps colonization by microorganisms and the relationship between such colonization and the type, length, diameter, and dura ... | 2011 | 22138843 |
| production of 3-hydroxypropionic acid through propionaldehyde dehydrogenase pdup mediated biosynthetic pathway in klebsiella pneumoniae. | the pdup gene encodes a propionaldehyde dehydrogenase (pdup) was investigated for the role in 3-hydroxypropionic acid (3-hp) glycerol metabolism in klebsiella pneumoniae. the enzyme assay showed that cell extracts from a pdup mutant strain lacked measurable dehydrogenase activity. additionally, the mutant strain accumulated the cytotoxic intermediate metabolite 3-hydroxypropionaldehyde (3-hpa), causing both cell death and a lower final 3-hp titer. ectopic expression of pdup restored normal cell ... | 2012 | 22018749 |
| prevalence and outcome of hepatobiliary dysfunction in neonatal septicaemia. | : cholestatic jaundice and liver enzyme abnormalities have been reported in neonatal septicaemia; the course, pattern, and outcome of such hepatobiliary dysfunction have not been described. | 2011 | 21873892 |
| the cita/citb two-component system regulating citrate fermentation in escherichia coli and its relation to the dcus/dcur system in vivo. | citrate fermentation by escherichia coli requires the function of the citrate/succinate antiporter citt (citt gene) and of citrate lyase (citcdefxg genes). earlier experiments suggested that the two-component system cita/citb, consisting of the membrane-bound sensor kinase cita and the response regulator citb, stimulates the expression of the genes in the presence of citrate, similar to cita/citb of klebsiella pneumoniae. in this study, the expression of a chromosomal citc-lacz gene fusion was s ... | 2011 | 22101843 |
| cefotaxime for the detection of extended-spectrum β-lactamase or plasmid-mediated ampc β-lactamase and clinical characteristics of cefotaxime-non-susceptible escherichia coli and klebsiella pneumoniae bacteraemia. | we investigated the performance of cefotaxime for the detection of extended-spectrum β-lactamase (esbl) or plasmid-mediated ampc β-lactamase (pampc) and the clinical characteristics of cefotaxime-non-susceptible escherichia coli or klebsiella pneumoniae (ctxns-ek) bacteraemia. all of the consecutive bloodstream isolates between 2005 and 2010 in a japanese university hospital were characterised using polymerase chain reaction (pcr). risk factors and outcomes of ctxns-ek were analysed by multivari ... | 2011 | 22210267 |
| structural segregation of gut microbiota between colorectal cancer patients and healthy volunteers. | despite a long-suspected role in the development of human colorectal cancer (crc), the composition of gut microbiota in crc patients has not been adequately described. in this study, fecal bacterial diversity in crc patients (n=46) and healthy volunteers (n=56) were profiled by 454 pyrosequencing of the v3 region of the 16s ribosomal rna gene. both principal component analysis and unifrac analysis showed structural segregation between the two populations. forty-eight operational taxonomic units ... | 2011 | 21850056 |
| colonisation and infection due to enterobacteriaceae producing plasmid-mediated ampc β-lactamases. | to investigate the epidemiology and clinical features of infections caused by enterobacteria producing plasmid-mediated ampc β-lactamases (pampc), which are emerging as a cause of resistance to extended-spectrum cephalosporins. | 2011 | 22138600 |
| The revival of fosfomycin. | Fosfomycin, originally named phosphonomycin, was discovered in Spain in 1969. There are three forms of fosfomycin: fosfomycin tromethamine (a soluble salt) and fosfomycin calcium for oral use, and fosfomycin disodium for intravenous use. Fosfomycin is a bactericidal antibiotic that interferes with cell wall synthesis in both Gram-positive and Gram-negative bacteria by inhibiting the initial step involving phosphoenolpyruvate synthetase. It has a broad spectrum of activity against a wide range of ... | 2011 | 21945848 |
| in vitro susceptibility of nosocomial isolates against a new aminoglycoside isepamicin. | isepamicin is a new aminoglycoside with a spectrum of activity similar to amikacin with an advantage of possessing a high level of stability against different aminoglycoside modifying enzymes. the in vitro susceptibility of nosocomial isolates obtained from patients admitted to the all india institute of medical sciences, new delhi during october-december, 1999 was tested against isepamicin and compared with other aminoglycosides. | 2001 | 21910285 |
| impact of restriction of cefepime use on the antimicrobial susceptibility of gram-negative bacilli related to healthcare-associated infections in an orthopedic hospital. | in recent decades, antimicrobial resistance has become a public health problem, particularly in cases of healthcare-associated infections. interaction between antibiotic consumption and resistance development is of particular interest regarding gram-negative bacilli, whose growing resistance has represented a great challenge. | 2011 | 21904460 |
| transient tlr activation restores inflammatory response and ability to control pulmonary bacterial infection in germfree mice. | mammals are colonized by an astronomical number of commensal microorganisms on their environmental exposed surfaces. these symbiotic species build up a complex community that aids their hosts in several physiological activities. we have shown that lack of intestinal microbiota is accompanied by a state of active il-10-mediated inflammatory hyporesponsiveness. the present study investigated whether the germfree state and its hyporesponsive phenotype alter host resistance to an infectious bacteria ... | 2011 | 22210917 |
| Extended spectrum â-lactamase producing multidrug resistant clinical bacterial isolates at National Public Health Laboratory, Nepal. | Extended Spectrum â-lactamase (ESBL) producing multidrug resistant bacteria complicate therapeutic management and limit treatment options. Therefore, detection of ESBL-producing multidrug resistant (MDR) pathogens has a paramount importance. Between April 2009 and January 2010, a prospective study was carried out in National Public Health Laboratory with an objective to determine the status of ESBL producing MDR bacterial isolates from different clinical samples. Identification of the isolates w ... | 2011 | 21991699 |
| Resistance to carbapenems in sequence type 11 Klebsiella pneumoniae is related to DHA-1 and loss of OmpK35 and/or OmpK36. | We investigated the molecular mechanisms of carbapenem resistance in clinical isolates of Klebsiella pneumoniae from Korea and the clinical outcomes of resistant K. pneumoniae infection. Sixteen K. pneumoniae isolates showing resistance to carbapenems collected from a tertiary-care hospital were examined for the mechanisms of carbapenem resistance. PCR and sequencing experiments detected the bla(DHA-1) AmpC ß-1actamase gene in all 16 clinical isolates, while the bla genes of extended-spectrum ß- ... | 2011 | 21940650 |
| Molecular typing and virulence analysis of serotype K1 Klebsiella pneumoniae strains isolated from liver abscess patients and stool samples from noninfectious subjects in Hong Kong, Singapore, and Taiwan. | Serotype K1 Klebsiella pneumoniae with multilocus sequence type 23 (ST23) has been strongly associated with liver abscess in Taiwan. Few data regarding the strain types and virulence of this serotype from other Asian countries are available. Serotype K1 K. pneumoniae strains isolated from liver abscess and stool samples from subjects hospitalized in Hong Kong, Singapore, and Taiwan hospitals were examined. Forty-seven serotype K1 isolates were identified: 26 from liver abscess samples and 21 fro ... | 2011 | 21900521 |
| Characterization and regulation of the 2,3-butanediol pathway in Serratia marcescens. | Serratia marcescens has been proved to be a potential strain for industrial 2,3-butanediol production for its high yield, productivity, and other advantages. In this study, the genes slaA, slaB, slaC, and slaR were successfully cloned which were further confirmed to be encoding acetolactate decarboxylase, acetolactate synthase, 2,3-butanediol dehydrogenase, and a LysR-like regulator, respectively. Unlike in Klebsiella sp. or Klebsiella pneumonie and Vibrio sp. or Vibrio cholerae, the gene slaC i ... | 2011 | 21983710 |
| asymptomatic bacteriuria among an obstetric population in ibadan. | asymptomatic bacteriuria in pregnancy is the major risk factor for symptomatic urinary tract infection during pregnancy. screening and identification of bacteriuria during pregnancy have been recommended. | 2011 | 21984454 |
| sulfoquinovose degraded by pure cultures of bacteria with release of c(3) -organosulfonates: complete degradation in two-member communities. | sulfoquinovose (sq, 6-deoxy-6-sulfoglucose) was synthesized chemically. an hplc-elsd method to separate sq and other chromophore-free sulfonates, e.g. 2,3-dihydroxypropane-1-sulfonate (dhps), was developed. a set of 10 genome-sequenced, sulfonate-utilizing bacteria did not utilize sq, but an isolate, pseudomonas putida sq1, from an enrichment culture did so. the molar growth yield with sq was half of that with glucose, and 1 mol 3-sulfolactate (mol sq)(-1) was formed during growth. the 3-sulfol ... | 2011 | 22150877 |
| chitosan and silver nanoparticles as pudding with raisins with antimicrobial properties. | chitosan nanoparticles (cs-np) containing small silver nanoparticles are reported (ag@cs-np). cs-np was synthesized using tripolyphosphate (tpp) as a polyanionic template. tpp also served to electrostatically attract ag(+) inside cs-np, where it was reduced by the terminal glucosamine units of the biopolymer. this procedure is environmental friendly, inexpensive, and permits the synthesis of very small agnp (0.93-1.7 nm), with only a discrete dependence from the amount of silver nitrate used (5- ... | 2011 | 21903218 |
| augmentation in zone of inhibition of cefoperazone/cefoperazone+ sulbactum compares well with the clinical laboratory standard institute standard extended spectrum beta-lactamase detection method as well as the polymerase chain reaction method. | with increased prevalence of extended spectrum beta-lactamase (esbl) in hospital practice globally, reporting of extended spectrum beta-lactamase along with drug susceptibility test is expected from clinical microbiology laboratory. the aim was to evaluate cefoperazone and cefoperazone+sulbactum disc for phenotypic detection of extended spectrum beta-lactamase among e. coli and klebsiella spp. isolates. | 2011 | 22122390 |
| [Carbapenem resistance in Klebsiella pneumoniae clinical isolates from urinary tract infections]. | Resistance to carbapenems by KPC (Klebsiella pneumoniae carbapenemase) production in Klebsiella pneumoniae clinical isolates was first described ten years ago in the U.S.A. and recently reported in other countries. This enzyme inactivates all beta-lactam antibiotics and is associated with fluoroquinolone and aminoglycoside resistance. | 2011 | 21870752 |
| bacteriuria and urinary retention following gynaecological surgery: comparing short vs long term catheterization. | post operative bladder drainage is important care following gynaecological surgeries. this study was done to compare the incidence of urinary retention and bacteriuria following long term versus short term catheterization. | 2010 | 21876574 |
| impact of inappropriate empirical antibiotic therapy on outcome of bacteremic adults visiting the ed. | objectives: to investigate the clinical impact of inappropriate empirical antibiotics on patient outcome and determine the risk factors for mortality in bacteremic adults who visited the emergency department (ed). methods: bacteremic adults visiting the ed from january 2007 to june 2008 were identified retrospectively. demographic characteristics, clinical conditions, bacteremic pathogens, antimicrobial agents, and outcomes were determined from chart records. results: the total of 454 eligible b ... | 2011 | 22205015 |
| prevalence of uropathogens in diabetic patients and their corresponding resistance pattern: results of a survey conducted at diagnostic centers in dhaka, bangladesh. | urinary tract infection is the second most common clinical indication for empirical antibiotic treatment in primary and secondary health care settings. the incidence of diabetes mellitus throughout the world is increasing strikingly and in the long run, it has some major effects on the genitourinary system which makes diabetic patients more liable to urinary tract infection. this study is designed to reveal the distribution of uropathogens in diabetic patients according to age and sex, and corre ... | 2010 | 22043358 |
| Risk factors and mortality for nosocomial bloodstream infections in elderly patients. | To determine risk factors for nosocomial bloodstream infection (BSI) and associated mortality in geriatric patients in geriatric and internal medicine wards at a university hospital. | 2011 | 21925041 |
| Molecular characteristics of KPC-producing Enterobacteriaceae at the early stage of their dissemination in Poland, 2008-2009. | After the first report in May 2008, the National Reference Center for Susceptibility Testing confirmed 113 cases of infection or colonization by KPC-producing members of the family Enterobacteriaceae in Poland by the end of 2009. The vast majority of patients were found in 18 hospitals; three patients were diagnosed at outpatient clinics. Most of the institutions were in the Warsaw area, including three hospitals with the highest numbers of cases. When available, the data on previous hospitaliza ... | 2011 | 21930889 |
| in vitro screening of circium arvense for potential antibacterial and antifungal activities. | the antimicrobial activities of circium arvense extracts were evaluated against two human gram positive (staphylococcus aureus, micrococcus luteus) and four gram negative pathogen (escherichia coli, pseudomonas aeruginosa, enterobacter, klebsiella pneumoniae). the methanol extracts of circium arvense were used to obtain various fractions (x1) n-hexane, (x2) chloroform, (x3) ethyl acetate and (x4) n-butanol. the agar well diffusion assay and agar dilution susceptibility testing were carried out t ... | 2011 | 21959814 |
| multilocus sequence typing compared to pfge for molecular typing of extended spectrum beta-lactamase-producing klebsiella pneumoniae isolates. | | 2011 | 22123581 |
| bacterial isolates in chronic suppurative otitis media: a changing pattern? | the most frequently isolated organism in chronic suppurative otitis media from different parts of the world is pseudomonas aeruginosa. the pattern from this bacteriological study from our region is different. this study was carried out on 97 patients presenting to the outpatient clinic of the national ear care center, kaduna. nigeria from may 2008 to april 2009. the patients were aged between 1 year and 75 years comprising 50) males and 47 females giving a male to female ratio of 1:1. most of th ... | 2011 | 21970268 |
| first detection of okp-a β-lactamase in two serratia marcescens isolates in china. | two strains of enterobacteriaceae producing prodigiosin were isolated from meat in the sichuan province of china in 2010. the strains were identified by vitek system, 16s rdna, rpob, pfs and luxs genes. minimum inhibitory concentrations were determined using the broth microdilution method. the two strains were screened for the presence of β-lactamase genes (blatem, blashv, blaokp, and blactx-m genes). based on pcr amplification and 16s rdna sequencing the analysed strains were identified as serr ... | 2011 | 22143810 |
| effect of a synbiotic on microbial community structure in a continuous culture model of the gastric microbiota in enteral nutrition patients. | patients with dysphagia require long-term nutritional support. this can be delivered by the enteral route via a percutaneous endoscopic gastrostomy (peg) tube. enteral nutrition (en) bypasses the body's innate defences that prevent the microbial colonization of the proximal gut, which predisposes to microbial overgrowth. a continuous culture model simulating the upper gastrointestinal tract microbiota of en patients was used to investigate the effects of a synbiotic (lactobacillus acidophilus du ... | 2011 | 22176141 |
| Outbreak due to a Klebsiella pneumoniae strain harbouring KPC-2 and VIM-1 in a German university hospital, July 2010 to January 2011. | We describe the epidemiology and characteristics of the pathogen and patients (n=7) associated with an outbreak of a carbapenem-resistant Klebsiella pneumoniae (CRKP) strain in a German university hospital from July 2010 to January 2011. Species identification and detection of carbapenem resistance were carried out using standard microbiological procedures. Carbapenemases were detected by phenotypic methods and specific polymerase chain reactions (PCRs). DNA fingerprinting profiles were performe ... | 2011 | 21871227 |
| Frequency of oral mucositis and microbiological analysis in children with acute lymphoblastic leukemia treated with 0.12% chlorhexidine gluconate. | In view of the morbidity potential of oral complications in patients with leukemia, this study evaluated the clinical and microbiological alterations that occur in the oral mucosa of children with acute lymphoblastic leukemia (ALL) undergoing antineoplastic chemotherapy and prophylactic administration of 0.12% chlorhexidine gluconate. The sample consisted of 17 children aged 2 to 12 years that underwent clinical examination of the oral mucosa for the detection of oral lesions. In addition, biolo ... | 2011 | 21861031 |
| [still increasing incidence of mrsa and esbl-producing enterobacteriaceae in society and in hospital-associated cases]. | this review describes the recent epidemiology for mrsa and esbl-producing enterobacteriaceae in denmark. mrsa community-associated cases continue to increase whereas hospital associated cases are low and stable. due to an active search and destroy policy secondary transmission is modest. mrsa from pigs is an increasing problem. for esbl producing escherichia coli a considerable multi clonal increase has been seen both in the community and in hospitals. there are indications on food being a signi ... | 2011 | 22053803 |
| trends in the susceptibility of clinically important resistant bacteria to tigecycline: results from the tigecycline in-vitro surveillance in taiwan (tist), 2006-2010. | the tigecycline in-vitro surveillance in taiwan (tist), a nationwide, prospective surveillance during 2006 to 2010, collected a total of 7,793 clinical isolates, including methicillin-resistant staphylococcus aureus (mrsa) (n=1,834), penicillin-resistant streptococcus pneumoniae (prsp) (n=423), vancomycin-resistant enterococci (vre) (n=219), extended-spectrum β-lactamase (esbl)-producing escherichia coli (n=1,141), esbl-producing klebsiella pneumoniae (n=1,330), acinetobacter baumannii (n=1,645) ... | 2011 | 22203598 |
| Risk factors and outcome of Pseudomonas aeruginosa bacteremia among adults visiting the ED. | OBJECTIVES: The aim of this study was to determine the clinical characteristics of, antibiotic therapy for, and clinical outcome of Pseudomonas aeruginosa infection among bacteremic adults who visit the emergency department (ED). METHODS: Bacteremic adults who visited the ED were identified retrospectively from January 2007 to December 2007. Demographic characteristics, underlying illnesses, severity, bacteremic pathogens, antimicrobial agents, and outcome were determined from chart records. RES ... | 2011 | 21855263 |
| Introduction of OXA-48-producing Enterobacteriaceae to Israeli hospitals by medical tourism. | The carbapenemase OXA-48 has been reported from different Mediterranean countries. It is mostly encoded on a single plasmid in various Enterobacteriaceae species. We characterized the epidemiological and molecular features of OXA-48-producing Enterobacteriaceae (OPE) in Israel. | 2011 | 22191089 |
| Research into new antibiotics is not the answer to resistance. | | 2011 | 22167783 |
| [distribution of extended spectrum β-lactamases-codifying genes in klebsiella pneumoniae isolates from hospitals of bogota, d.c., colombia]. | extended spectrum β-lactamases (esbl) are the most widely distributed enzymes in enterobacteriaceae of latin america and are key enzymes in resistance to antibiotics in common use. however, in colombia, little information is available concerning the identity of genes coding for these enzymes in klebsiella pneumoniae. | 2011 | 22159479 |
| hypervirulent k. pneumoniae secretes more and more active iron-acquisition molecules than "classical" k. pneumoniae thereby enhancing its virulence. | a new hypervirulent (hypermucoviscous) clinical variant of klebsiella pneumoniae (hvkp) has emerged over the last decade. our goal is to identify new mechanisms, which increase the virulence hvkp compared to "classic" k. pneumoniae (ckp). | 2011 | 22039542 |
| Intercontinental transfer of OXA-181-producing Klebsiella pneumoniae into New Zealand. | | 2011 | 21933789 |
| The age-related risk of co-existing meningitis in children with urinary tract infection. | The primary aim of this study was to determine age-stratified rates of co-existing bacterial meningitis in children with urinary tract infection (UTI). The secondary aims of this study were to determine the causative pathogens of UTI, and the clinical features and outcome of children with co-existing meningitis. | 2011 | 22096488 |
| [Establishment of loop-mediated isothermal amplification technique for rapid detection of NDM-1 gene]. | We established a rapid detection method of New Delhi Metallo-beta-Lactamase Gene (NDM-1) based on Loop-mediated Isothermal Amplification (LAMP). With the application of LAMP, we designed four sets of LAMP premiers, using NDM-1 gene as the target sequence, and selected the set of optimal primers. Meanwhile, we established optimal reaction systems and conditions to carry out the sensitivity and specificity experiments. The experiment results showed that the whole detection process took only one ho ... | 2011 | 22097813 |
| Carbapenem-resistant Klebsiella pneumoniae associated with a long-term--care facility --- West Virginia, 2009-2011. | On January 27, 2011, a West Virginia county health department was notified of a cluster of carbapenem-resistant Klebsiella pneumoniae (CRKP) cases detected by a local hospital (hospital A). CRKP infections frequently are resistant to a majority of antimicrobial agents and have an increased risk for morbidity and mortality. The West Virginia Bureau for Public Health (WVBPH) conducted field investigations to identify all cases, characterize risk factors for infection, and abstract data for a match ... | 2011 | 22012114 |
| th17 cells mediate clade-specific, serotype-independent mucosal immunity. | the interleukin-17 (il-17) family of cytokines phylogenetically predates the evolution of t cells in jawed vertebrates, suggesting that the ontogeny of the th17 cell lineage must have arisen to confer an evolutionary advantage to the host over innate sources of il-17. utilizing a model of mucosal immunization with the encapsulated bacteria klebsiella pneumoniae, we found that b cells, which largely recognized polysaccharide capsular antigens, afforded protection to only the vaccine strain. in co ... | 2011 | 22195749 |
| clinical and phenotypic differences between classic and hypervirulent klebsiella pneumonia: an emerging and under-recognized pathogenic variant. | the purpose of this study was to increase awareness, gain insight into acquisition, and assess the virulence of the hypervirulent (hypermucoviscous) clinical variant (hvkp) that is entrenched in the pacific rim but emerging in western countries. a case of community-acquired liver abscess with metastatic spread to the spleen is described. comparative in vitro and in vivo virulence studies on this isolate (hvkp1) and four randomly chosen blood isolates of "classic" k. pneumonia strains (ckp1-4) we ... | 2011 | 21918907 |
| TLR4-DEPENDENT GM-CSF PROTECTS AGAINST LUNG INJURY IN GRAM-NEGATIVE BACTERIAL PNEUMONIA. | Toll like receptors are required for protective host defense against bacterial pathogens. However, the role of TLRs in regulating lung injury during Gram negative bacterial pneumonia has not been thoroughly investigated. In this study, experiments were performed to evaluate the role of TLR4 in pulmonary responses against Klebsiella pneumoniae (Kp). As compared to WT (Balb/c) mice, mice with defective TLR4 signaling (TLR4(lps-d) mice) had substantially higher lung bacterial CFU after i.t. challen ... | 2011 | 22160309 |
| Appearance of Klebsiella pneumoniae liver abscess syndrome in Argentina: case report and review of molecular mechanisms of pathogenesis. | Klebsiella pneumoniae liver abscess syndrome (KLAS) is an emerging invasive infection caused by highly virulent community-acquired strains of K. pneumoniae displaying hypermucoviscosity. The salient features of this syndrome include the presence of bacteremia, primary monomicrobial liver abscess, and metastatic complications. A previously healthy Argentinean man presented with fever and found to have liver abscess caused by K. pneumoniae with metastatic seeding of gastric wall. Cultures from blo ... | 2011 | 22145012 |
| Septic arthritis as a presentation of Crohn's disease. | A 42-year-old man with colonic Crohn's disease presented to the orthopaedic department with septic arthritis of the knee with frank intra-articular pus, which persisted despite repeated joint washouts. This coincided with a symptomatic flare of his colonic Crohns. Imaging revealed a fistulous connection from the proximal sigmoid colon into the pelvis, a psoas abscess, myositis of the musculature around the left pelvic girdle and left thigh and two focal collections almost surrounding the distal ... | 2011 | 21977716 |
| Enzymatic hydrolysis and simultaneous saccharification and fermentation of alkali/peracetic acid-pretreated sugarcane bagasse for ethanol and 2,3-butanediol production. | The enzymatic digestibility of alkali/peracetic acid (PAA)-pretreated bagasse was systematically investigated. The effects of initial solid consistency, cellulase loading and addition of supplemental ß-glucosidase on the enzymatic conversion of glycan were studied. It was found the alkali-PAA pulp showed excellent enzymatic digestibility. The enzymatic glycan conversion could reach about 80% after 24 h incubation when enzyme loading was 10 FPU/g solid. Simultaneous saccharification and fermentat ... | 2011 | 22112569 |
| efficacies of colistin and tigecycline in mice with experimental pneumonia due to ndm-1-producing strains of klebsiella pneumoniae and escherichia coli. | new delhi metallo-β-lactamase-1 (ndm-1)-producing enterobacteriaceae have emerged as a global threat. the aim of this study was to assess the efficacies of colistin and tigecycline in an experimental model of pneumonia caused by ndm-1-producing escherichia coli and klebsiella pneumoniae. the susceptibilities of k. pneumoniae ndm, e. coli ndm and k. pneumoniae atcc 29665 were determined using the broth microdilution technique. the pharmacokinetics of colistin and tigecycline in an experimental mo ... | 2011 | 22154856 |
| Histopathologic evaluation of lung and extrapulmonary tissues show sex differences in Klebsiella pneumoniae - infected mice under different exposure conditions. | It has been shown that female mice with pneumonia have a survival advantage over males, but this is reversed if ozone exposure precedes infection. The purpose of this study was to investigate factors that underlie these observations, by studying histopathologic changes in lung and extrapulmonary (spleen and liver) tissues after ozone or filtered air (FA) exposure followed by pulmonary bacterial infection. Male and female wild type C57BL/6J mice were exposed to ozone or FA, then anesthetized and ... | 2011 | 21941609 |