| examination of mgatp binding in a tryptophan-shift mutant of phosphofructokinase from bacillus stearothermophilus. | a tryptophan-shift variant of bacillus stearothermophilus phosphofructokinase (bspfk), w179f/f76w, was constructed to evaluate the binding and allosteric characteristics associated with mgatp. w179f/f76w bspfk has a specific activity of 77+/-1 u/mg at ph 7 and 25 degrees c, which is a 35% decrease compared to the wild-type enzyme. the k(m) for mgatp increases from 43+/-3 microm for wild-type bspfk to 160+/-7 microm in the variant. binding and allosteric interaction between fru-6-p and pep for th ... | 2005 | 15752723 |
| identification of a gene cluster encoding an arginine atp-binding-cassette transporter in the genome of the thermophilic gram-positive bacterium geobacillus stearothermophilus strain dsmz 13240. | a single gene cluster encoding components of a putative atp-binding cassette (abc) transporter for basic amino acids was identified in the incomplete genome sequence of the thermophilic gram-positive bacterium geobacillus stearothermophilus by blast searches. the cluster comprises three genes, and these were amplified from chromosomal dna of g. stearothermophilus, ligated into plasmid vectors and expressed in escherichia coli. the purified solute-binding protein (designated artj) was demonstrate ... | 2005 | 15758229 |
| the dependence of the sporicidal effects on the power and pressure of rf-generated plasma processes. | the sporicidal effect of 20 different radio-frequency plasma processes produced by combining five different gas mixtures [o(2), ar/h(2) (50/50%), ar/h(2) (5/95%), o(2)/h(2) (50/50%), o(2)/h(2) (95/5%)] with four power/pressure settings were tested. sporicidal effects of oxygen-containing plasmas were dependent on power at low pressure settings but not at high pressure settings. in the absence of oxygen no power dependency was observed at either high or low pressure settings. survivor curves obta ... | 2005 | 15765503 |
| simulation and experiment at high temperatures: ultrafast folding of a thermophilic protein by nucleation-condensation. | we used phi-value analysis to characterise the transition state for folding of a thermophilic protein at the relatively high temperature of 325 k. phif values for the folding of the three-helix bundle, peripheral subunit binding domain from bacillus stearothermophilus (e3bd) were determined by temperature-jump experiments in the absence of chemical denaturants. e3bd folded in microseconds through a highly diffuse transition state. excellent agreement was observed between experiment and the resul ... | 2005 | 15769475 |
| protein photo-cross-linking in mammalian cells by site-specific incorporation of a photoreactive amino acid. | we report a method of photo-cross-linking proteins in mammalian cells, which is based on site-specific incorporation of a photoreactive amino acid, p-benzoyl-l-phenylalanine (pbpa), through the use of an expanded genetic code. to analyze the cell signaling interactions involving the adaptor protein grb2, pbpa was incorporated in its src homology 2 (sh2) domain. the human grb2 gene with an amber codon was introduced into chinese hamster ovary (cho) cells, together with the genes for the bacillus ... | 2005 | 15782189 |
| studies on bacillus stearothermophilus. part iv. influence of enhancers on biotransformation of testosterone. | the impact of chemical enhancers on the biotransformation of testosterone has been exploited. application of crude cell concentrates to produce bacillus stearothermophilus-mediated bioconversion of testosterone at 65 degrees c for 72 h has been examined. after incubation, the xenobiotic substrate was added to the concentrated whole cell suspensions. the enhancer molecules were included in the whole cell suspension. the resultant products, after extraction into an organic solvent, were purified b ... | 2005 | 15784287 |
| evaluating the formulae for integrated lethality in ethylene oxide sterilization using six different endospore forming strains of bacteria, and comparisons of integrated lethality for ethylene oxide and steam systems. | bacterial endospores from six different species of bacteria were exposed to a spectrum of ethylene oxide (eto) sterilizing conditions. temperature was varied from 40 to 60 degrees c and the ethylene oxide concentration was varied from 300 to 750 mg/l. relative humidity was maintained at 60+/-10% rh. the fraction negative procedure was used to determine the d value for each of the test conditions. bacterial species tested included bacillus atrophaeus atcc # 9372, bacillus smithii atcc # 51232, ba ... | 2005 | 15796136 |
| effect of a y265f mutant on the transamination-based cycloserine inactivation of alanine racemase. | the requirement for d-alanine in the peptidoglycan layer of bacterial cell walls is fulfilled in part by alanine racemase (ec 5.1.1.1), a pyridoxal 5'-phosphate (plp)-assisted enzyme. the enzyme utilizes two antiparallel bases focused at the c(alpha) position and oriented perpendicular to the plp ring to facilitate the equilibration of alanine enantiomers. understanding how this two-base system is utilized and controlled to yield reaction specificity is therefore a potential means for designing ... | 2005 | 15807525 |
| kinetic analysis about the effects of neutral salts on the thermal stability of yeast alcohol dehydrogenase. | the effects of salts on the rate constants of inactivation by heat of yeast alcohol dehydrogenase (yadh) at 60.0 degrees c were measured. different effects were observed at low and high salt concentrations. at high concentrations, some salts had stabilizing effects, while others were destabilizing. the effects of salts in the high concentration range examined can be described as follows: (decreased thermal stability) naclo(4) < nai = (c(2)h(5))(4)nbr < nh(4)br < nabr = kbr = csbr = (no addition) ... | 2005 | 15809336 |
| enzymatic synthesis of a new inhibitor of alpha-amylases: acarviosinyl-isomaltosyl-spiro-thiohydantoin. | synthesis of acarviosinyl-isomaltosyl-spiro-thiohydantoin in yields up to 20%, has been achieved by bacillus stearothermophilus maltogenic amylase (bsma). bsma is capable of transferring the acarviosine-glucose residue from an acarbose donor onto glucopyranosylidene-spiro-thiohydantoin. reactions were followed using hplc and maldi-tof ms. 1h and 13c nmr studies revealed that the enzyme reserved its stereoselectivity. glycosylation took place mainly at c-6 resulting in alpha-acarviosinyl-(1-->4)- ... | 2005 | 15854600 |
| phluorin-based in vivo assay for hydrolase screening. | phluorin, a ph-sensitive mutant of green fluorescent protein (gfp), acts as a sensor for intracellular ph shifts, triggered by hydrolytic enzymes. this principle was used to develop a phluorin-based in vivo assay for hydrolase screening. the presented assay was evaluated for escherichia coli (e. coli) cells, producing heterologous phluorin and an esterase from geobacillus stearothermophilus which is considered as a model hydrolase. subsequently, the utility of this detection system was also demo ... | 2005 | 15859586 |
| role of lys-226 in the catalytic mechanism of bacillus stearothermophilus serine hydroxymethyltransferase--crystal structure and kinetic studies. | serine hydroxymethyltransferase (shmt), a pyridoxal 5'-phosphate (plp)-dependent enzyme catalyzes the reversible conversion of l-ser and tetrahydropteroylglutamate (h(4)pteglu) to gly and 5,10-methylene tetrahydropteroylglutamate (ch(2)-h(4)pteglu). biochemical and structural studies on this enzyme have implicated several residues in the catalytic mechanism, one of them being the active site lysine, which anchors plp. it has been proposed that this residue is crucial for product expulsion. howev ... | 2005 | 15865438 |
| a superhelical spiral in the escherichia coli dna gyrase a c-terminal domain imparts unidirectional supercoiling bias. | dna gyrase is unique among type ii topoisomerases in that its dna supercoiling activity is unidirectional. the c-terminal domain of the gyrase a subunit (gyra-ctd) is required for this supercoiling bias. we report here the x-ray structure of the escherichia coli gyra-ctd (protein data bank code 1zi0). the e. coli gyra-ctd adopts a circular-shaped beta-pinwheel fold first seen in the borrelia burgdorferi gyra-ctd. however, whereas the b. burgdorferi gyra-ctd is flat, the e. coli gyra-ctd is spira ... | 2005 | 15897198 |
| discovery of antagonist peptides against bacterial helicase-primase interaction in b. stearothermophilus by reverse yeast three-hybrid. | developing small-molecule antagonists against protein-protein interactions will provide powerful tools for mechanistic/functional studies and the discovery of new antibacterials. we have developed a reverse yeast three-hybrid approach that allows high-throughput screening for antagonist peptides against essential protein-protein interactions. we have applied our methodology to the essential bacterial helicase-primase interaction in bacillus stearothermophilus and isolated a unique antagonist pep ... | 2005 | 15911380 |
| histidines 345 and 378 of bacillus stearothermophilus leucine aminopeptidase ii are essential for the catalytic activity of the enzyme. | the conserved histidine residues, his-191, his-227, his-345, and his-378, in bacillus stearothermophilus leucine aminopeptidase ii (lapii) were replaced with leucine by site-directed mutagenesis. the overexpressed wild-type and mutant enzymes have been purified by nickel-chelate chromatography and their molecular masses were approximately 44.5 kda. under assay conditions, no lap activity was detected in h345l and h378l. although the km value for h191l increased more than 30% with respect to the ... | 2005 | 15928987 |
| the bacterial helicase-primase interaction: a common structural/functional module. | the lack of a high-resolution structure for the bacterial helicase-primase complex and the fragmented structural information for the individual proteins have been hindering our detailed understanding of this crucial binary protein interaction. two new structures for the helicase-interacting domain of the bacterial primases from escherichia coli and bacillus stearothermophilus have recently been solved and both revealed a unique and surprising structural similarity to the amino-terminal domain of ... | 2005 | 15939015 |
| zinc in lipase l1 from geobacillus stearothermophilus l1 and structural implications on thermal stability. | lipase l1 from geobacillus stearothermophilus l1 contains an unusual extra domain, making a tight intramolecular interaction with the main catalytic domain through a zn2+-binding coordination. to elucidate the role of the zn2+, we disrupted the zn2+-binding site by mutating the zinc-ligand residues (h87a, d61a/h87a, and d61a/h81a/h87a/d238a). the activity vs. temperature profiles of the mutant enzymes showed that the disruption of the zn2+-binding site resulted in a notable decrease in the optim ... | 2005 | 15949807 |
| a comparative study of ethylene oxide gas, hydrogen peroxide gas plasma, and low-temperature steam formaldehyde sterilization. | to compare the efficacies of ethylene oxide gas (eog), hydrogen peroxide gas plasma (plasma), and low-temperature steam formaldehyde (ltsf) sterilization methods. | 2005 | 15954488 |
| does incineration turn infectious waste aseptic? | incineration of infectious waste is considered to be biologically safe. we performed basic experiments to confirm that bacillus spores are killed by incineration in a muffle furnace. biological samples containing 10(6) spores of bacillus stearothermophilus were placed in stainless steel petri dishes and then into hot furnaces. the furnace temperature and duration of incineration were 300 degrees c for 15 min, 300 degrees c for 30 min, 500 degrees c for 15 min, 500 degrees c for 30 min and 1100 d ... | 2005 | 15963601 |
| hyperthermophilic alpha-l: -arabinofuranosidase from thermotoga maritima msb8: molecular cloning, gene expression, and characterization of the recombinant protein. | a putative alpha-l: -arabinofuranosidase (afase) gene belonging to family 51 of glycosyl hydrolases of a hyperthermophilic bacterium thermotoga maritima msb8 was cloned, sequenced, and overexpressed in escherichia coli. the recombinant protein (tm-afase) was purified to apparent homogeneity by heat treatment (80 degrees c, 30 min), followed by hydrophobic interaction, anion-exchange, and gel permeation column chromatography. tm-afase had a molecular mass of 55,284 da on matrix assisted laser des ... | 2005 | 15965714 |
| tet(l)-mediated tetracycline resistance in bovine mannheimia and pasteurella isolates. | tetracycline-resistant mannheimia and pasteurella isolates, which were negative for the tetracycline resistance genes (tet) commonly detected among these bacteria, were investigated for other tet genes present and their location. | 2005 | 15972309 |
| interactions of the n-terminal domain of ribosomal protein l11 with thiostrepton and rrna. | ribosomal protein l11 has two domains: the c-terminal domain (l11-c76) binds rrna, whereas the n-terminal domain (l11-ntd) may variously interact with elongation factor g, the antibiotic thiostrepton, and rrna. to begin to quantitate these interactions, l11 from bacillus stearothermophilus has been overexpressed and its properties compared with those of l11-c76 alone in a fluorescence assay for protein-rrna binding. the assay relies on 2'-amino-butyryl-pyrene-uridine incorporated in a 58-nucleot ... | 2005 | 15972821 |
| identifying and engineering ion pairs in adenylate kinases. insights from molecular dynamics simulations of thermophilic and mesophilic homologues. | molecular dynamics simulations were performed to study thermal stabilization of proteins via electrostatic interactions of ion pairs. dynamic motions of four ion pairs previously proposed to be important in thermal stability of adenylate kinase from the thermophile bacillus stearothermophilus were monitored during the simulation. one of the four ion pairs identified in the crystal structure, lys180-asp114, was not maintained in close contact suggesting that the ion pair does not contribute to th ... | 2005 | 15995248 |
| structures of tryptophanyl-trna synthetase ii from deinococcus radiodurans bound to atp and tryptophan. insight into subunit cooperativity and domain motions linked to catalysis. | an auxiliary tryptophanyl trna synthetase (drtrprs ii) that interacts with nitric-oxide synthase in the radiation-resistant bacterium deinococcus radiodurans charges trna with tryptophan and 4-nitrotryptophan, a specific nitration product of nitric-oxide synthase. crystal structures of drtrprs ii, empty of ligands or bound to either trp or atp, reveal that drtrprs ii has an overall structure similar to standard bacterial trprss but undergoes smaller amplitude motions of the helical trna anti-cod ... | 2005 | 15998643 |
| the binding interface between bacillus stearothermophilus ribosomal protein s15 and its 5'-translational operator mrna. | the bacillus stearothermophilus ribosomal protein s15 (bs15) binds a purine-rich three-helix junction motif in the central domain of 16s ribosomal rna (rrna) as well as a translational operator located in the 5'-untranslated region (5'-utr) of its cognate messenger rna (mrna). an in-frame fusion between the 5'-utr of the bs15 gene and beta-galactosidase (lacz) was prepared, and tested for bs15-dependent translational repression of lacz activity in escherichia coli. the presence of bs15 in trans ... | 2005 | 16005889 |
| a modified consensus approach to mutagenesis inverts the cofactor specificity of bacillus stearothermophilus lactate dehydrogenase. | lactate dehydrogenase from bacillus stearothermophilus is specific for nad+. there have been several attempts to alter the cofactor specificity of this enzyme, but these have yielded enzymes with relatively low activities that still largely prefer nad+. a modified consensus approach was used to create a library of phylogenetically preferred amino acids situated near the cofactor binding site, and variants were screened for their ability to utilize nmn+. a triple mutant (mut31) was discovered tha ... | 2005 | 16012175 |
| the sterility of hospital-prepared soffban bandages. | an evaluation of the sterility of hospital-prepared soffban bandages was undertaken. discs of bacillus stearothermophilus were inserted into the bandage rolls, prior to sterilization in "porous load" autoclaves. the discs were subsequently removed and placed in culture media, with growth of the organism indicating failure of sterilization. it was demonstrated that soffban could not be sterilized reliably using standard hospital autoclave techniques. | 2005 | 16019112 |
| comparison of various assays used for detection of beta-lactam antibiotics in poultry meat. | in this study, microbiological tests for the detection of beta-lactam antibiotics in meat and meat products were evaluated. the traditional fpt (four plate test, containing bacillus subtilis and kocuria rhizophila), bsda (bacillus stearothermophilus disc assay) and a newly developed microbiological test, premi test (containing bacillus stearothermophilus) were included in the study. the limit of detection (lod) of the premi test was compared with the lod of the traditional methods. the detection ... | 2005 | 16019829 |
| autoregulated, bidirectional and multicistronic gas-inducible mammalian as well as lentiviral expression vectors. | we present a novel set of autoregulated, bidirectional and multicistronic mammalian as well as lentiviral expression vectors which enable transgene expression fine-tuning by gaseous acetaldehyde. the acetaldehyde-inducible regulation (air) technology capitalizes on aspergillus nidulans components evolved to convert ethanol into metabolic energy. air is based on functional interaction of the fungal transactivator alcr and alcr-specific chimeric promoters (p(air)) which drive desired transgene exp ... | 2005 | 16026881 |
| antibiotic residues in goats milk following intramammary treatment. | two lactational intramammary antibiotic preparations (cloxacillin and oxytetracycline) were each used to treat eight goats by intramammary infusion in one half. the rate of release of the antibiotics was monitored by a diffusion assay based on the sensitive organism bacillus stearothermophilus var. calidolactis. cloxacillin was still detectable 156 hours after the final treatment while oxytetracycline was undetectable 108 hours following treatment. a small but significant amount of antibiotic wa ... | 1984 | 16031077 |
| secretion of heterologous proteins in bacillus subtilis can be improved by engineering cell components affecting post-translocational protein folding and degradation. | to explore the potential to enhance secretion of heterologous proteins in bacillus subtilis by engineering cell factors affecting extracytoplasmic protein folding and degradation. | 2005 | 16033468 |
| tagatose production by immobilized recombinant escherichia coli cells containing geobacillus stearothermophilus l-arabinose isomerase mutant in a packed-bed bioreactor. | using immobilized recombinant escherichia coli cells containing geobacillus stearothermophilus l-arabinose isomerase mutant (gali 152), we found that the galactose isomerization reaction was maximal at 70 degrees c and ph 7.0. manganese ion enhanced galactose isomerization to tagatose. the immobilized cells were most stable at 60 degrees c and ph 7.0. the cell and substrate concentrations and dilution rate were optimal at 34 g/l, 300 g/l, and 0.05 h(-1), respectively. under the optimum condition ... | 2005 | 16080720 |
| toxicity of methoprene as assessed by the use of a model microorganism. | methoprene is an insect juvenile growth hormone mimic, commonly used as a pesticide. although widely used for the control of several pests, toxic effects on organisms of different phyla have been reported. these events triggered studies to clarify the mechanisms of toxicity of this insecticide putatively involved in ecological issues. here we show the effect of methoprene on the normal cell growth and viability of a strain of the thermophilic eubacterium bacillus stearothermophilus, previously u ... | 2005 | 16081242 |
| opposite roles of domains 2+3 of escherichia coli ef-tu and bacillus stearothermophilus ef-tu in the regulation of ef-tu gtpase activity. | the effect of noncatalytic domains 2+3 on the intrinsic activity and thermostability of the ef-tu gtpase center was evaluated in experiments with isolated domains 1 and six chimeric variants of mesophilic escherichia coli (ec) and thermophilic bacillus stearothermophilus (bst) ef-tus. the isolated catalytic domains 1 of both ef-tus displayed similar gtpase activities at their optimal temperatures. however, noncatalytic domains 2+3 of the ef-tus influenced the gtpase activity of domains 1 differe ... | 2005 | 16081328 |
| solution structure of the c1-subdomain of bacillus stearothermophilus translation initiation factor if2. | if2 is one of three bacterial translation initiation factors that are conserved through all kingdoms of life. it binds the 30s and 50s ribosomal subunits, as well as fmet-trnaf(met). after these interactions, fmet-trnaf(met) is oriented to the ribosomal p-site where the first amino acid of the nascent polypeptide, formylmethionine, is presented. the c-terminal domain of bacillus stearothermophilus if2, which is responsible for recognition and binding of fmet-trnaf(met), contains two structured m ... | 2005 | 16081655 |
| screening of catalytically active microorganisms for the synthesis of 6-modified purine nucleosides. | modified nucleosides can be prepared by microbial transglycosylation from cheaper nucleoside precursors using free or immobilised whole cells. an efficient screening method to find transglycosylation activity in microorganisms was developed for the synthesis of 6-modified purine nucleosides, such as 6-chloro-, 6-methoxy-, 6-iodo- and 6-mercaptopurine ribonucleoside. out of 100 microorganisms screened, bacillus stearothermophilus atcc 12980 was the best for this purpose. | 2005 | 16086256 |
| structure and hydride transfer mechanism of a moderate thermophilic dihydrofolate reductase from bacillus stearothermophilus and comparison to its mesophilic and hyperthermophilic homologues. | dihydrofolate reductase (dhfr) from a moderate thermophilic organism, bacillus stearothermophilus, has been cloned and expressed. physical characterization of the protein (bsdhfr) indicates that it is a monomeric protein with a molecular mass of 18,694.6 da (0.8), coincident with the mass of 18 694.67 da calculated from the primary sequence. determination of the x-ray structure of bsdhfr provides the first structure for a monomeric dhfr from a thermophilic organism, indicating a high degree of c ... | 2005 | 16114879 |
| heat capacity-independent determination of differential free energy of stability between structurally homologous proteins. | under the assumption of equivalent heat capacity values, the differential free energy of stability for a pair of proteins midway between their thermal unfolding transition temperatures is shown to be independent of deltac(p) up to its cubic term in deltat(m). for model calculations reflecting the nearly 30 degrees c difference in t(m) for the adenylate kinases from the arctic bacterium bacillus globisporus and the thermophilic bacterium geobacillus stearothermophilus, the resultant error in esti ... | 2006 | 16125837 |
| proteomics viewed on stress response of thermophilic bacterium bacillus stearothermophilus tls33. | thermophilic bacterium bacillus stearothermophilus tls33, isolated from a hot spring in chiang mai, thailand, usually produces many enzymes that are very useful for industrial applications. however, the functional properties and mechanisms of this bacterium under stress conditions are rarely reported and still need more understanding on how the bacterium can survive in stress environments. in this study, we examined the oxidative stress induced proteins of this bacterium by proteomic approach co ... | 2005 | 16127733 |
| crystal structure of a bacterial ribonuclease p rna. | the x-ray crystal structure of a 417-nt ribonuclease p rna from bacillus stearothermophilus was solved to 3.3-a resolution. this rna enzyme is constructed from a number of coaxially stacked helical domains joined together by local and long-range interactions. these helical domains are arranged to form a remarkably flat surface, which is implicated by a wealth of biochemical data in the binding and cleavage of the precursors of transfer rna substrate. previous photoaffinity crosslinking data are ... | 2005 | 16157868 |
| decontamination assessment of bacillus anthracis, bacillus subtilis, and geobacillus stearothermophilus spores on indoor surfaces using a hydrogen peroxide gas generator. | to evaluate the decontamination of bacillus anthracis, bacillus subtilis, and geobacillus stearothermophilus spores on indoor surface materials using hydrogen peroxide gas. | 2005 | 16162224 |
| thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of bacillus stearothermophilus us100 strain. | the implications of asn315 and val450 in the atypical starch hydrolysis profile of bacillus stearothermophilus amy (a-amylase) us100 have been suggested previously [ben ali, mhiri, mezghani and bejar (2001) enzyme microb. tech. 28, 537-542]. in order to confirm this hypothesis, three mutants were generated. of these two have a single mutation, n315d or v450g, whereas the third contains both mutations. analysis of the starch breakdown-profile of these three mutants, as well as of the wild-type, a ... | 2006 | 16197365 |
| enzyme-substrate complex structures of a gh39 beta-xylosidase from geobacillus stearothermophilus. | beta-d-xylosidases are glycoside hydrolases that catalyse the release of xylose units from short xylooligosaccharides and are engaged in the final breakdown of plant cell-wall hemicelluloses. beta-d-xylosidases are found in glycoside hydrolase families 3, 39, 43, 52 and 54. the first crystal structure of a gh39 beta-xylosidase revealed a multi-domain organization with the catalytic domain having the canonical (beta/alpha)8 barrel fold. here, we report the crystal structure of the gh39 geobacillu ... | 2005 | 16212978 |
| an inexpensive and rapid diagnostic method of koi herpesvirus (khv) infection by loop-mediated isothermal amplification. | koi herpesvirus (khv) affects both juvenile and adult common carp and koi, and is especially lethal to fry. the high mortalities caused by the disease have had a negative impact on the international koi trade. different diagnostic techniques have been used to detect khv, including: isolation of the virus in cell culture, electron microscopy, several pcr tests, elisa and in situ hybridisation. all of these methods are time consuming, laborious and require specialised equipment. | 2005 | 16216123 |
| application of artificial neural networks to describe the combined effect of ph and nacl on the heat resistance of bacillus stearothermophilus. | a model for prediction of bacterial spore inactivation was developed. the influence of temperature, ph and nacl on the heat resistance of bacillus stearothermophilus spores was described using low-complexity, black box models based on artificial neural networks. literature data were used to build and train the neural network, and new experimental data were used to evaluate it. the neural network models gave better predictions than the classical quadratic response surface model in all the experim ... | 2006 | 16216369 |
| functional proteomics and correlated signaling pathway of the thermophilic bacterium bacillus stearothermophilus tls33 under cold-shock stress. | the thermophilic bacterium bacillus stearothermophilus tls33 was examined under cold-shock stress by a proteomic approach to gain a better understanding of the protein synthesis and complex regulatory pathways of bacterial adaptation. after downshift in the temperature from 65 degrees c, the optimal growth temperature for this bacterium, to 37 degrees c and 25 degrees c for 2 h, we used the high-throughput techniques of proteomic analysis combining 2-de and ms to identify 53 individual proteins ... | 2005 | 16222717 |
| direct correlationship between proton translocation and growth yield: an analysis of the respiratory chain of bacillus stearothermophilus. | thermophilic bacilli contain cytochrome caa3-type cytochrome c oxidase as the main terminal oxidase in the respiratory chain. a mutant strain, named k-17, lacking cytochrome caa3 and exhibiting very low n,n,n',n'-tetramethyl-p-phenylene diamine oxidase activity, was isolated by random mutation from bacillus stearothermophilus k1041 (sakamoto, j. et al., fems microbiol. lett., 143, 151-158, 1996). comparing this mutant with the parent strain k1041, we observed the following differences in energy- ... | 1999 | 16232504 |
| enzymatic synthesis of fructose 1,6-diphosphate with atp regeneration in a batch reactor and a semibatch reactor using purified enzymes of bacillus stearothermophilus. | the enzymatic synthesis of fructose 1,6-diphosphate (fdp), an important glycolytic intermediate whose applications in the field of medicine have generated a great deal of interest, was performed in a batch reactor and a semibatch reactor. using the batch reactor, fdp was first synthesized from glucose by three enzymatic reactions and the atp consumed was regenerated simultaneously using conjugated enzymes, all of which were purified from crude cell extract of thermophilic bacillus stearothermoph ... | 1999 | 16232527 |
| enzymatic production of fructose 1,6-diphosphate using crude cell extract of bacillus stearothermophilus. | the enzymatic production of fructose 1,6-diphosphate (fdp) from glucose was performed in a batch reactor and a semibatch reactor using the crude cell extract of bacillus stearothermophilus which contains all four enzymes required for the synthesis. the experimental results of the yield and the time courses of fdp production obtained using various enzyme concentrations were in good agreement with the theoretical predictions calculated based on the differential equations including the rate equatio ... | 1999 | 16232540 |
| a new sterility test for cow's milk using alanine racemase gene as the index. | oligonucleotide primers designed from consensus sequences of alanine racemase genes were used for a sterility test of cow's milk by polymerase chain reaction (pcr). commercial cow's milk in two 250 ml packages was separately centrifuged at 5000 x g for 10 min, bacterial cells in each precipitate were cultivated at 30 and 55 degrees c for 5 h in luria-bertani medium, and the cells from each culture were mixed and used for the pcr after being treated with 0.1 n naoh at 60 degrees c for 10 min. whe ... | 1999 | 16232568 |
| experimental investigation of fructose 1,6-diphosphate production and simultaneous atp regeneration by conjugated enzymes in an ultrafiltration hollow-fiber reactor. | the enzymatic synthesis of fructose 1,6-diphosphate (fdp) from glucose and the enzymatic atp regeneration were performed simultaneously in an ultrafiltration hollow-fiber reactor using both the purified enzymes and the crude cell extract of bacillus stearothermophilus. the process consisted of the three-step synthetic reactions catalyzed by glucokinase, phosphoglucose isomerase and phosphofructokinase, and the atp regeneration reaction catalyzed by acetate kinase. the experimental results of the ... | 1999 | 16232677 |
| antimicrobial effects of green tea polyphenols on thermophilic spore-forming bacteria. | the inhibitory action of tea polyphenols towards the development and growth of bacterial spores was examined. among the tested bacillus bacteria, tea polyphenols showed antibacterial effects towards bacillus stearothermophilus, which is a thermophilic spore-forming bacterium. the heat resistance of b. stearothermophilus spores was reduced by the addition of tea polyphenols. clostridium thermoaceticum, an anaerobic spore-forming bacterium, also exhibited reduced heat resistance of its spores in t ... | 2000 | 16232822 |
| cell surface engineering of yeast: construction of arming yeast with biocatalyst. | a cell surface engineering system of yeast saccharomyces cerevisiae has been established and novel yeasts armed by biocatalysts (enzymes-glucoamylase, alpha-amylase, cm-cellulase, beta-glucosidase, and lipase), termed "arming yeasts", were constructed. the gene encoding rhizopus oryzae glucoamylase with its secretion signal peptide was fused with the gene encoding the c-terminal half of yeast alpha-agglutinin and expressed in s. cerevisiae. glucoamylase was shown to be displayed on the cell surf ... | 2000 | 16232831 |
| isolation of a thermophile degrading poly(butylene succinate-co-butylene adipate). | a thermophile, strain 73, which degrades poly(butylene succinate-co-butylene adipate) (pbsa) film was isolated from 95 soil samples obtained from different locations by cultivation using an enrichment culture technique at 60 degrees c. at this temperature, the strain grew on pbsa and the dissolved total organic carbon (toc) concentration in the medium changed according to the growth stage, i.e., after the toc concentration rapidly reached the minimum, it increased rapidly until it reached a peak ... | 2000 | 16232871 |
| importance of an n-terminal extension in ribonuclease hii from bacillus stearothermophilus for substrate binding. | the gene encoding ribonuclease hii from bacillus stearothermophilus was cloned and expressed in escherichia coli. the overproduced protein, bst-rnase hii, was purified and biochemically characterized. bst-rnase hii, which consists of 259 amino acid residues, showed the highest amino acid sequence identity (50.2%) to bacillus subtilis rnase hii. like b. subtilis rnase hii, it exhibited mn2+-dependent rnase h activity. it was, however, more thermostable than b. subtilis rnase hii. when the bst-rna ... | 2002 | 16233183 |
| purification, characterization and gene cloning of thermostable o-acetyl-l-serine sulfhydrylase forming beta-cyano-l-alanine. | a thermophilic and cyanide ion-tolerant bacterium, bacillus stearothermophilus cn3 isolated from a hot spring in japan, was found to produce thermostable beta-cyano-l-alanine synthase. the enzyme catalyzes the synthesis of beta-cyano-l-alanine from o-acetyl-l-serine and cyanide ions. the purified enzyme has a molecular mass of approximately 70 kda and consists of two identical subunits. it was stable in the ph range of 6.0 to 10.0 and up to 70 degrees c. the enzyme also catalyzes the synthesis o ... | 2003 | 16233442 |
| synthesis of glycosyl glycerol by cyclodextrin glucanotransferases. | glycerol was transglycosylated by cyclodextrin glucanotransferases using starch as a donor substrate. among the enzymes tested, those from geobacillus stearothermophilus and thermoanaerobacter sp. were suitable for the transglycosylation. several products were isolated and their structures were elucidated. they were composed of glucose and a series of a-1,4-linked maltooligosyl residues bound with glycerol. o-alpha-d-glucosyl-(1-->1)-glycerol and o-alpha-d-glucosyl-(1-->2)-glycerol were identifi ... | 2003 | 16233461 |
| purification, characterization and gene cloning of thermostable o-acetyl-l-homoserine sulfhydrylase forming gamma-cyano-alpha-aminobutyric acid. | a thermophilic and cyanide ion-tolerant bacterium, bacillus stearothermophilus cn3 isolated from a hot spring in japan, was found to produce thermostable gamma-cyano-alpha-aminobutyric acid synthase. the enzyme was purified and characterized. the purified enzyme has a molecular mass of approximately 180 kda and consists of four identical subunits. it was stable in the ph range of 6.0 to 10.5 and up to 60 degrees c. the enzyme catalyzed the gamma-replacement reaction of o-acetyl-l-homo-serine wit ... | 2003 | 16233482 |
| insight into the roles of tyrosine 82 and glycine 253 in the escherichia coli adenine glycosylase muty. | the oxidation product of 2'-deoxyguanosine, 7,8-dihydro-8-oxo-2'-deoxyguanosine (og), produces g:c to t:a transversion mutations. the escherichia coli base excision repair glycosylase muty plays an important role in preventing og-associated mutations by removing adenines misincorporated opposite og lesions during dna replication. recently, biallelic mutations in the human muty homologue (hmyh) have been correlated with the development of colorectal cancer. the two most common mutations correspon ... | 2005 | 16245934 |
| heterologous protein production capacity of mammalian cells cultivated as monolayers and microtissues. | a precise understanding of processes managing heterologous protein production in vitro and in vivo is essential for the manufacture of sophisticated biopharmaceuticals as well as for future gene therapy and tissue engineering initiatives. capitalizing on the gravity-enforced self-assembly of monodispersed cells into coherent (multicellular) microtissues we studied heterologous protein production of microtissues and monolayers derived from cell lines and primary cells engineered/transduced for (i ... | 2006 | 16255048 |
| inactivation of bacillus spores by the combination of moderate heat and low hydrostatic pressure in ketchup and potage. | the combination effect of moderate heat and low hydrostatic pressure (mhp) on the reduction of bacillus subtilis, bacillus coagulans and geobacillus stearothermophilus spores in food materials (potage and ketchup) was investigated. these bacterial spores were suspended in potage (ph 7), acidified potage (ph 4), neutralized ketchup (ph 7) and ketchup (ph 4). the suspensions were treated with and without pressure (100 mpa) and temperatures of 65-85 degrees c for 3 to 12 h. the bacterial spores wer ... | 2006 | 16260058 |
| phylogenetic analysis and biochemical characterization of a thermostable dihydropyrimidinase from alkaliphilic bacillus sp. ts-23. | two degenerate primers established from the alignment of highly conserved amino acid sequences of bacterial dihydropyrimidinases (dhps) were used to amplify a 330-bp gene fragment from the genomic dna of bacillus sp. ts-23 and the amplified dna was successfully used as a probe to clone a dhp gene from the strain. the open reading frame of the gene consisted of 1422 bp and was deduced to contain 472 amino acids with a molecular mass of 52 kda. the deduced amino acid sequence exhibited greater tha ... | 2005 | 16284925 |
| kinetic and structural characterization of phosphofructokinase from lactobacillus bulgaricus. | phosphofructokinase from lactobacillus delbrueckii subspecies bulgaricus (lbpfk) has been reported to be a nonallosteric analogue of phosphofructokinase from escherichia coli at ph 8.2 [le bras et al. (1991) eur. j. biochem. 198, 683-687]. a reexamination of the kinetics of this enzyme shows lbpfk to have limited binding affinity toward the allosteric ligands, mgadp and pep, with dissociation constants of approximately 20 mm for both. their allosteric effects are observed only at high concentrat ... | 2005 | 16285731 |
| osmotic shock: a mechanosensitive channel blocker can prevent release of cytoplasmic but not periplasmic proteins. | when over-expressed in the cytoplasm of escherichia coli, carboxylesterase est55 of geobacillus stearothermophilus was found to be released from cells upon osmotic shock. comparing two osmotic shock protocols showed that release of est55 was abolished in the absence of mechanosensitive channel mscl by one method but not the other. the discrepancy extended to several previously reported cytoplasmic proteins released by osmotic shock, including: ef-tu, thioredoxin, and dnak in e. coli. stepwise an ... | 2005 | 16288836 |
| identification of biogenic dimethyl selenodisulfide in the headspace gases above genetically modified escherichia coli. | escherichia coli jm109 cells were modified to express the genes encoded in a 3.8-kb chromosomal dna fragment from a metalloid-resistant thermophile, geobacillus stearothermophilus v. manual headspace extraction was used to collect the gases for gas chromatography with fluorine-induced sulfur chemiluminescence analysis while solid-phase microextraction was used for sample collection in gas chromatography/mass spectrometry (gc/ms) analysis. when grown in the presence of selenate or selenite, these ... | 2006 | 16289446 |
| molecular structure of a 9-mda icosahedral pyruvate dehydrogenase subcomplex containing the e2 and e3 enzymes using cryoelectron microscopy. | the pyruvate dehydrogenase multienzyme complexes are among the largest multifunctional catalytic machines in cells, catalyzing the production of acetyl coa from pyruvate. we have previously reported the molecular architecture of an 11-mda subcomplex comprising the 60-mer icosahedral dihydrolipoyl acetyltransferase (e2) decorated with 60 copies of the heterotetrameric (alpha(2)beta(2)) 153-kda pyruvate decarboxylase (e1) from bacillus stearothermophilus (milne, j. l. s., shi, d., rosenthal, p. b. ... | 2006 | 16308322 |
| characterization of a thermoacidophilic l-arabinose isomerase from alicyclobacillus acidocaldarius: role of lys-269 in ph optimum. | the araa gene encoding l-arabinose isomerase (ai) from the thermoacidophilic bacterium alicyclobacillus acidocaldarius was cloned, sequenced, and expressed in escherichia coli. analysis of the sequence revealed that the open reading frame of the araa gene consists of 1,491 bp that encodes a protein of 497 amino acid residues with a calculated molecular mass of 56,043 da. comparison of the deduced amino acid sequence of a. acidocaldarius ai (aaai) with other ais demonstrated that aaai has 97% and ... | 2005 | 16332764 |
| construction and expression of an ethanol production operon in gram-positive bacteria. | pyruvate decarboxylase (pdc), an enzyme central to homoethanol fermentation, catalyses the non-oxidative decarboxylation of pyruvate to acetaldehyde with release of carbon dioxide. pdc enzymes from diverse organisms have different kinetic properties, thermal stability and codon usage that are likely to offer unique advantages for the development of desirable gram-positive biocatalysts for use in the ethanol industry. to examine this further, pdc genes from bacteria to yeast were expressed in the ... | 2005 | 16339947 |
| crystal structure and structure-based mutational analyses of rnase hiii from bacillus stearothermophilus: a new type 2 rnase h with tbp-like substrate-binding domain at the n terminus. | ribonuclease hiii (bst-rnase hiii) from the moderate thermophile bacillus stearothermophilus is a type 2 rnase h but shows poor amino acid sequence identity with another type 2 rnase h, rnase hii. it is composed of 310 amino acid residues and acts as a monomer. bst-rnase hiii has a large n-terminal extension with unknown function and a unique active-site motif (dede), both of which are characteristics common to rnases hiii. to understand the role of these n-terminal extension and active-site res ... | 2006 | 16343535 |
| effect of soybean casein digest agar lot on number of bacillus stearothermophilus spores recovered. | in recent years it has become increasingly apparent that bacillus stearothermophilus spores are affected by various environmental factors that influence the performance of the spores as biological indicators. one environmental factor is the recovery medium. the effect of different lots of commercial soybean casein digest agar on the number of colony-forming units per plate was examined in two series of experiments: (i) several lots of medium from two manufacturers were compared in single experim ... | 1981 | 16345822 |
| cloning and expression of thermostable alpha-amylase gene from bacillus stearothermophilus in bacillus stearothermophilus and bacillus subtilis. | the structural gene for a thermostable alpha-amylase from bacillus stearothermophilus was cloned in plasmids ptb90 and ptb53. it was expressed in both b. stearothermophilus and bacillus subtilis. b. stearothermophilus carrying the recombinant plasmid produced about fivefold more alpha-amylase (20.9 u/mg of dry cells) than did the wild-type strain of b. stearothermophilus. some properties of the alpha-amylases that were purified from the transformants of b. stearothermophilus and b. subtilis were ... | 1983 | 16346413 |
| reproduction of bacillus stearothermophilus as a function of temperature and pressure. | the colony-forming ability and the rate of reproduction of bacillus stearothermophilus were determined as a function of temperature and pressure. colonies were formed between 39 and 70 degrees c at atmospheric pressure and between 54 and 67 degrees c at 45 mpa. colonies did not form at 55.9 mpa. the rate of reproduction in broth cultures decreased with increasing pressure at all temperatures. the rate of reproduction diminished rapidly with pressure above 10.4 mpa. therefore, increased hydrostat ... | 1983 | 16346444 |
| high production of thermostable beta-galactosidase of bacillus stearothermophilus in bacillus subtilis. | by cloning the beta-galactosidase gene of bacillus stearothermophilus iam11001 (atcc 8005) into bacillus subtilis, enzyme production was enhanced 50 times. beta-galactosidase could be purified to 80% homogeneity by incubating the cell extract of b. subtilis at 70 degrees c for 15 min, followed by centrifugation to remove the denatured proteins. because of its heat stability and ease of production, beta-galactosidase is suitable for application in industrial processes. | 1985 | 16346825 |
| culture conditions for production of thermostable amylase by bacillus stearothermophilus. | bacillus stearothermophilus grew better on complex and semisynthetic medium than on synthetic medium supplemented with amino acids. amylase production on the complex medium containing beef extract or corn steep liquor was higher than on semisynthetic medium containing peptone (0.4%). the synthetic medium, however, did not provide a good yield of extracellular amylase. among the carbohydrates which favored the production of amylase are, in order starch > dextrin > glycogen > cellobiose > maltohex ... | 1986 | 16347107 |
| purification and characterization of thermostable pullulanase from bacillus stearothermophilus and molecular cloning and expression of the gene in bacillus subtilis. | a thermostable pullulanase (alpha-dextrin 6-glucanohydrolase [ec 3.2.1.41]) from a newly isolated bacillus stearothermophilus strain (trs128) was purified and characterized. the enzyme hydrolyzed (1-->6)-alpha-d-glucosidic linkages of pullulan to produce maltotriose, and the optimum temperature was 65 degrees c. about 90% of the enzyme activity was retained after treatment at 65 degrees c for 60 min. by using ptb522 as a vector plasmid, the pullulanase gene was cloned and expressed in bacillus s ... | 1988 | 16347785 |
| useful host-vector systems in bacillus stearothermophilus. | we isolated a highly transformable thermophile, bacillus stearothermophilus sic1, which exhibited the following features. the growth temperature ranged from 45 to 65 degrees c in l broth. the maximum cell concentration in 2l broth (2% tryptone, 1% yeast extract, 0.5% nacl, ph 7.2) was determined as an optical density at 660 nm of 7.8, and the generation time was 11 min at 60 degrees c. strain sic1 was a prototroph and was transformed by the protoplast procedure not only with repb plasmids (high- ... | 1988 | 16347805 |
| heat shock affects permeability and resistance of bacillus stearothermophilus spores. | [this corrects the article on p. 2517 in vol. 54.]. | 1989 | 16347831 |
| thermostable, raw-starch-digesting amylase from bacillus stearothermophilus. | an endospore-forming thermophilic bacterium, which produced amylase and was identified as bacillus stearothermophilus, was isolated from soil. the amylase had an optimum temperature of 70 degrees c and strongly degraded wheat starch granules (93%) and potato starch granules (80%) at 60 degrees c. | 1989 | 16347958 |
| mathematical model of thermal destruction of bacillus stearothermophilus spores. | the experimental survival curves of bacillus stearothermophilus spores in aqueous suspension, for six constant temperatures ranging from 105 to 130 degrees c, displayed an initial shoulder before a linear decline. to interpret these observations, we supposed that, before the heat treatment, the designated spore suspension contained a countable and mortal n(0) population of activated spores and an m(0) population of dormant spores which remained masked during spore counting and had to be activate ... | 1990 | 16348314 |
| fluorimetric detection of a bacillus stearothermophilus spore-bound enzyme, alpha-d-glucosidase, for rapid indication of flash sterilization failure. | a biological indicator based on fluorimetric detection within 60 min of a bacillus stearothermophilus spore-bound enzyme, alpha-d-glucosidase, has been developed. results indicate that the enzyme survived slightly longer than spores observed after 24 h of incubation. the new system shows promise for evaluating flash sterilization cycles within 60 min compared with conventional 24-h systems. | 1992 | 16348654 |
| spore production by bacillus stearothermophilus. | [this corrects the article on p. 690 in vol. 14.]. | 1967 | 16349719 |
| [determination and analysis of the primary structure of nm.bstsei operone from bacillus stearothermophilus se-589 which produces n.bstsei site-specific nickase]. | nucleotide sequence of bacillus stearothermophilus se-589 dna fragment which includes an operone for site-specific nm-system with a gene for bstsei nickase has been determined. analysis of the regions adjacent to nickase gene has revealed two genes encoding dna methyltransferases, which belong to different classes. three genes which form system operone are separated with short open reading frames (orfs). analysis of these orfs has shown that they encode polypeptides which are homologous to diffe ... | 2005 | 16358732 |
| comparison of two radio-frequency plasma sterilization processes using microspot evaluation of microbial inactivation. | in this study, we evaluated gas plasma surface sterilization methods in a specific sterilizer. we have introduced a new monitoring method using 0.4 microm pore size membranes, which in this study gave the information corresponding to 3000 exposed biological indicators per treatment cycle. this enabled us to compare the fraction of inoculates that showed no growth after exposure for 30 different locations in the chamber, and hereby identify weak and strong spots in the chamber with regard to spor ... | 2006 | 16362959 |
| cloning, purification and biochemical characterization of metallic-ions independent and thermoactive l-arabinose isomerase from the bacillus stearothermophilus us100 strain. | the araa gene encoding l-arabinose isomerase from bacillus stearothermophilus us100 strain was cloned, sequenced and over-expressed in e. coli. this gene encodes a 496-amino acid protein with a calculated molecular weight of 56.161 kda. its amino acid sequence displays the highest identity with l-ai from thermus sp. im6501 (98%) and that of geobacillus stearothermophilus t6 (97%). according to sds-page analysis, under reducing and non-reducing conditions, the recombinant enzyme has an apparent m ... | 2006 | 16386851 |
| expression of the ubie gene of geobacillus stearothermophilus v in escherichia coli k-12 mediates the evolution of selenium compounds into the headspace of selenite- and selenate-amended cultures. | the ubie gene of geobacillus stearothermophilus v, with its own promoter, was cloned and introduced into escherichia coli. the cloned gene complemented the ubie gene deficiency of e. coli an70. in addition, the expression of this gene in e. coli jm109 resulted in the evolution of volatile selenium compounds when these cells were grown in selenite- or selenate-amended media. these compounds were dimethyl selenide and dimethyl diselenide. | 2006 | 16391146 |
| structure and reactivity of a thermostable prokaryotic nitric-oxide synthase that forms a long-lived oxy-heme complex. | in an effort to generate more stable reaction intermediates involved in substrate oxidation by nitric-oxide synthases (noss), we have cloned, expressed, and characterized a thermostable nos homolog from the thermophilic bacterium geobacillus stearothermophilus (gsnos). as expected, gsnos forms nitric oxide (no) from l-arginine via the stable intermediate n-hydroxy l-arginine (noha). the addition of oxygen to ferrous gsnos results in long-lived heme-oxy complexes in the presence (soret peak 427 n ... | 2006 | 16407211 |
| in the bacillus stearothermophilus dnab-dnag complex, the activities of the two proteins are modulated by distinct but overlapping networks of residues. | we demonstrate the primase activity of bacillus stearothermophilus dnag and show that it initiates at 3'-atc-5' and 3'-att-5' sites synthesizing primers that are 22 or 23 nucleotides long. in the presence of the helicase dnab the size distribution of primers is different, and a range of additional smaller primers are also synthesized. nine residues from the n- and c-terminal domains of dnab, as well as its linker region, have been reported previously to affect this interaction. in bacillus stear ... | 2006 | 16452437 |
| biosynthesis of a thermostable gellan lyase by newly isolated and characterized strain of geobacillus stearothermophilus 98. | the thermophilic strain able to degrade gellan was isolated from bulgarian hot spring. according to its morphological and biochemical properties and by partial sequencing of its 16s rdna, it was classified as geobacillus stearothermophilus. it grew in a synthetic medium with gellan as the only carbon source with a specific growth rate of 0.69 h(-1) and generation time of 60 min. the strain produced thermostable gellan lyase extracellularly during exponential phase. its synthesis was inducible; t ... | 2006 | 16482399 |
| comparison of hydrogen peroxide and peracetic acid as isolator sterilization agents in a hospital pharmacy. | the efficacy of hydrogen peroxide and peracetic acid as isolator sterilization agents was compared. | 2006 | 16484519 |
| molecular cloning and characterization of an enzyme hydrolyzing p-nitrophenyl alpha-d-glucoside from bacillus stearothermophilus sa0301. | bacillus stearothermophilus sa0301 produces an extracellular oligo-1,6-glucosidase (bso16g) that also hydrolyzes p-nitrophenyl alpha-d-glucoside (tonozuka et al., j. appl. glycosci., 45, 397-400 (1998)). we cloned a gene for an enzyme hydrolyzing p-nitrophenyl alpha-d-glucoside, which was different from the one mentioned above, from b. stearothermophilus sa0301. the k(0)/k(m) values of bso16g for isomaltotriose and isomaltose were 13.2 and 1.39 s(-1).mm(-1) respectively, while the newly cloned e ... | 2006 | 16495668 |
| crystallization and preliminary x-ray diffraction study of thermostable rnase hiii from bacillus stearothermophilus. | a thermostable ribonuclease hiii from bacillus stearothermophilus (bst rnase hiii) was crystallized and preliminary crystallographic studies were performed. plate-like overlapping polycrystals were grown by the sitting-drop vapour-diffusion method at 283 k. native x-ray diffraction data were collected to 2.8 a resolution using synchrotron radiation from station bl44xu at spring-8. the crystals belong to the orthorhombic space group p2(1)2(1)2, with unit-cell parameters a = 66.73, b = 108.62, c = ... | 2005 | 16511022 |
| crystallization and preliminary x-ray analysis of pyruvate kinase from bacillus stearothermophilus. | pyruvate kinase (pk) from a moderate thermophile, bacillus stearothermophilus (bstpk), is an allosteric enzyme activated by amp and ribose 5-phosphate but not by fructose 1,6-bisphosphate (fbp). however, almost all other pks are activated by fbp. the wild-type and w416f/v435w mutant bstpks were crystallized by the hanging-drop vapour-diffusion method. however, they were unsuitable for structural analysis because their data sets exhibited low completeness. a crystal suitable for structural analys ... | 2005 | 16511150 |
| crystallization and preliminary crystallographic analysis of a family 43 beta-d-xylosidase from geobacillus stearothermophilus t-6. | beta-d-xylosidases (ec 3.2.1.37) are hemicellulases that cleave single xylose units from the nonreducing end of xylooligomers. in this study, the crystallization and preliminary x-ray analysis of a beta-d-xylosidase from geobacillus stearothermophilus t-6 (xynb3), a family 43 glycoside hydrolase, is described. xynb3 is a 535-amino-acid protein with a calculated molecular weight of 61 891 da. purified recombinant native and catalytic inactive mutant proteins were crystallized and cocrystallized w ... | 2005 | 16511233 |
| crystallization and preliminary x-ray diffraction studies of two thermostable alpha-galactosidases from glycoside hydrolase family 36. | alpha-galactosidases from thermophilic organisms have gained interest owing to their applications in the sugar industry. the alpha-galactosidases agaa, agab and agaa a355e mutant from geobacillus stearothermophilus have been overexpressed in escherichia coli. crystals of agab and agaa a355e have been obtained by the vapour-diffusion method and synchrotron data have been collected to 2.0 and 2.8 a resolution, respectively. crystals of agab belong to space group i222 or i2(1)2(1)2(1), with unit-ce ... | 2006 | 16511274 |
| crystallization and preliminary crystallographic analysis of the catechol 2,3-dioxygenase pheb from bacillus stearothermophilus br219. | class ii extradiol-cleaving catecholic dioxygenase, a key enzyme of aromatic compound degradation in bacteria, cleaves the aromatic ring of catechol by adding two o atoms. pheb is one of the class ii extradiol-cleaving catecholic dioxygenases and shows a high substrate specificity for catechol derivatives, which have one aromatic ring. in order to reveal the mechanism of the substrate specificity of pheb, pheb has been crystallized by the hanging-drop vapour-diffusion method using peg 4000 as a ... | 2006 | 16511281 |
| mathematical models for the effects of ph, temperature, and sodium chloride on the growth of bacillus stearothermophilus in salty carrots. | estimating the shelf life and safety of any food product is an important part of food product development. predictive food microbiology reduces the time and expense associated with conventional challenge and shelf life testing. the purpose of this study was to characterize and model germination, outgrowth, and lag (gol) time and the exponential growth rate (egr) of bacillus stearothermophilus in salty carrot medium (scm) as a function of ph, temperature, and nacl concentration. b. stearothermoph ... | 1997 | 16535566 |
| stabilization of enzymes against thermal stress and freeze-drying by mannosylglycerate. | 2-o-(beta)-mannosylglycerate, a solute that accumulates in some (hyper)thermophilic organisms, was purified from pyrococcus furiosus cells, and its effect on enzyme stabilization in vitro was assessed. enzymes from hyperthermophilic, thermophilic, and mesophilic sources were examined. the thermostabilities of alcohol dehydrogenases from p. furiosus and bacillus stearothermophilus and of glutamate dehydrogenases from thermotoga maritima and clostridium difficile were improved to a significant ext ... | 1997 | 16535713 |
| an enzymatically produced novel cyclomaltopentaose cyclized from amylose by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. | a bacterial strain am7, isolated from soil and identified as bacillus circulans, produced two kinds of novel cyclic oligosaccharides. the cyclic oligosaccharides were produced from amylose using a culture supernatant of the strain as the enzyme preparation. the major product was a cyclomaltopentaose cyclized by an alpha-(1-->6)-linkage, cyclo-{-->6)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->4)-alpha-d-glcp-(1-->}. the other minor product was cyclomaltohexao ... | 2006 | 16545346 |
| efficacy of supplementation of alpha-amylase-producing bacterial culture on the performance, nutrient use, and gut morphology of broiler chickens fed a corn-based diet. | a trial was conducted to evaluate the efficacy of an escherichia coli strain producing alpha-amylase of bacillus stearothermophilus on growth performance, nutrient use, and the morphology of the small intestine of broilers fed a corn-based diet. one hundred thirty-five 1-d-old chicks (cobb 500) were randomly divided into 3 groups and treated as follows: (i) basal diet (control); (ii) basal diet and water supplemented with an e. coli strain that produced amylase, and (iii) basal diet and water su ... | 2006 | 16553283 |
| amino acid sequence around the catalytic site in glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus. | the tryptic peptide containing the active-site cysteine in glyceraldehyde-3-phosphate dehydrogenase from bacillus stearothermophilus 1503 was isolated after inhibition of the enzyme with (14)c-iodoacetate. the amino acid sequence of the 20-residue peptide was determined by 19 successive cycles of dansyl-edman degradation. the sequence shows considerable homology with its counterparts from mesophilic sources but differs by the addition of ala-his-his at the n-terminus and by the substitution of p ... | 1972 | 16559157 |