| therapeutic implication of 'iturin a' for targeting md-2/tlr4 complex to overcome angiogenesis and invasion. | tumor angiogenesis and invasion are deregulated biological processes that drive multistage transformation of tumors from a benign to a life-threatening malignant state activating multiple signaling pathways including md-2/tlr4/nf-κb. development of potential inhibitors of this signaling is emerging area for discovery of novel cancer therapeutics. in the current investigation, we identified iturin a (a lipopeptide molecule from bacillus megaterium) as a potent inhibitor of angiogenesis and cancer ... | 2017 | 28347875 |
| a b12-dependent radical sam enzyme involved in oxetanocin a biosynthesis. | oxetanocin a (oxt-a) is a potent antitumour, antiviral and antibacterial compound. biosynthesis of oxt-a has been linked to a plasmid-borne bacillus megaterium gene cluster that contains four genes: oxsa, oxsb, oxra and oxrb. here we show that both the oxsa and oxsb genes are required for the production of oxt-a. biochemical analysis of the encoded proteins, a cobalamin (cbl)-dependent s-adenosylmethionine (adomet) radical enzyme, oxsb, and an hd-domain phosphohydrolase, oxsa, reveals that oxt-a ... | 2017 | 28346939 |
| a plate method for rapid screening of ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production. | a new plate method was developed for rapid screening of ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-klg). the screening methodology took the advantage of the acidity caused by 2-klg, which changes the color of bromothymol blue (ph indicator) from blue to yellow. using the proposed method, a mutant, k. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. when co-cultured with bacillus megaterium 2980 in 20-l ferment ... | 2017 | 28292630 |
| a new group of glycoside hydrolase family 13 α-amylases with an aberrant catalytic triad. | α-amylases are glycoside hydrolase enzymes that act on the α(1→4) glycosidic linkages in glycogen, starch, and related α-glucans, and are ubiquitously present in nature. most α-amylases have been classified in glycoside hydrolase family 13 with a typical (β/α)8-barrel containing two aspartic acid and one glutamic acid residue that play an essential role in catalysis. an atypical α-amylase (bman1) with only two of the three invariant catalytic residues present was isolated from bacillus megateriu ... | 2017 | 28287181 |
| characterization of polyhydroxyalkanoate produced by bacillus megaterium vb89 isolated from nisargruna biogas plant. | polyhydroxyalkanoates (phas) are naturally occurring biodegradable polymers that can curb the extensive use of polypropylene based plastics. in contrast to chemically synthesized polypropylene plastics, phas are biodegradable and thus environmentally safe. phas have attracted much attention as biocompatible and biodegradable thermoplastics. the present study involves isolation of bacteria from different environments capable of synthesizing phas. the bacterium producing highest yield of pha (0.67 ... | 2017 | 28283932 |
| efficient synthesis of (s)-n-boc-3-hydroxypiperidine using an (r)-specific carbonyl reductase from candida parapsilosis. | (s)-n-boc-3-hydroxypiperidine (s-nbhp) is a critical chiral intermediate in the synthesis of pharmaceuticals, including ibrutinib, the active pharmaceutical ingredient of the new drug imbruvica approved for the treatment of lymphoma. an (r)-specific carbonyl reductase from candida parapsilosis (cprcr, also known as r-specific alcohol dehydrogenase) that catalyzes asymmetric reduction to produce (s)-n-boc-3-hydroxypiperidine (s-nbhp) was identified for the first time. when co-expressed with a glu ... | 2017 | 28243985 |
| co-inoculation effect of rhizobia and plant growth promoting rhizobacteria on common bean growth in a low phosphorus soil. | nitrogen (n) fixation through legume-rhizobium symbiosis is important for enhancing agricultural productivity and is therefore of great economic interest. growing evidence indicates that other soil beneficial bacteria can positively affect symbiotic performance of rhizobia. nodule endophytic plant growth promoting rhizobacteria (pgpr) were isolated from common bean nodules from nakuru county in kenya and characterized 16s rdna partial gene sequencing. the effect of co-inoculation of rhizobium an ... | 2017 | 28224000 |
| combined chemical and physical transformation method with rbcl and sepiolite for the transformation of various bacterial species. | dna transformation that delivers plasmid dnas into bacterial cells is fundamental in genetic manipulation to engineer and study bacteria. developed transformation methods to date are optimized to specific bacterial species for high efficiency. thus, there is always a demand for simple and species-independent transformation methods. we herein describe the development of a chemico-physical transformation method that combines a rubidium chloride (rbcl)-based chemical method and sepiolite-based phys ... | 2017 | 28185866 |
| engineering of cyp106a2 for steroid 9α- and 6β-hydroxylation. | cyp 106a2 from bacillus megaterium atcc 13368 has been described as a 15β-hydroxylase showing also minor 11α-, 9α- and 6β-hydroxylase activity for progesterone conversion. previously, mutant proteins with a changed selectivity towards 11α-oh-progesterone have already been produced. the challenge of this work was to create mutant proteins with a higher regioselectivity towards hydroxylation at positions 9 and 6 of the steroid molecule. 9α-hydroxyprogesterone exhibits pharmaceutical importance, be ... | 2017 | 28163026 |
| antimicrobial effects of novel peptides cot2 and sot2 derived from crocodylus siamensis and pelodiscus sinensis ovotransferrins. | in light of the increasing threat of bacterial drug resistance to human health on a global scale, research and development of antimicrobial peptides as a novel class of potent antibiotics has gained considerable attention. the present study focuses on the structural evaluation and membrane interaction of two new cationic antimicrobial peptides, cot2 and sot2, derived from siamese crocodile (crocodylus siamensis) and chinese softshell turtle (pelodiscus sinensis) ovotransferrins. here, cot1 (+3) ... | 2017 | 28159460 |
| in vitro study of biological activity of four strains of burkholderia gladioli pv. agaricicola and identification of their bioactive metabolites using gc-ms. | this research was carried out to study in vitro antibacterial activity of 4 strains of burkholderia gladioli pv. agaricicola (bga) against g+ve bacillus megaterium and g-ve escherichia coli, haemolytic activity against the cell membrane of erythrocytes, the production of extracellular hydrolytic enzymes and finally, the pathogenicity against agaricus bisporus flesh blocks. chemical structure of bioactive substances of the most bioactive strain (icmp 11096) was established using gas chromatograph ... | 2017 | 28149165 |
| role of microbial inoculation and industrial by-product phosphogypsum in growth and nutrient uptake of maize (zea mays l.) grown in calcareous soil. | alkaline soils with high calcium carbonate and low organic matter are deficient in plant nutrient availability. use of organic and bio-fertilizers has been suggested to improve their properties. therefore, a greenhouse experiment was conducted to evaluate the integrative role of phosphogypsum (pg; added at 0.0, 10, 30, and 50 g pg kg(-1) ), cow manure (cm; added at 50 g kg(-1) ) and mixed microbial inoculation (incl.; azotobacter chroococcum, and phosphate-solubilizing bacteria bacillus megateri ... | 2017 | 28106264 |
| hydrocarbon-stapled lipopeptides exhibit selective antimicrobial activity. | antimicrobial peptides (amps) occur widely in nature and have been studied for their therapeutic potential. amps are of interest due to the large number of possible chemical structural combinations using natural and unnatural amino acids, with varying effects on their biological activities. using physicochemical properties from known naturally occurring amphipathic cationic amps, several hydrocarbon-stapled lipopeptides (hslps) were designed, synthesized, and tested for antimicrobial properties. ... | 2017 | 28073163 |
| production and anti-melanoma activity of methoxyisoflavones from the biotransformation of genistein by two recombinant escherichia coli strains. | biotransformation of the soy isoflavone genistein by sequential 3'-hydroxylation using recombinant escherichia coli expressing tyrosinase from bacillus megaterium and then methylation using another recombinant e. coli expressing o-methyltransferase from streptomyces peucetius was conducted. the results showed that two metabolites were produced from the biotransformation, identified as 5,7,4'-trihydroxy-3'-methoxyisoflavone and 5,7,3'-trihydroxy-4'-methoxyisoflavone, respectively, based on their ... | 2017 | 28054996 |
| characterization of cytochrome p450 cyp109e1 from bacillus megaterium as a novel vitamin d3 hydroxylase. | in this study the ability of cyp109e1 from bacillus megaterium to metabolize vitamin d3 (vd3) was investigated. in an in vitro system using bovine adrenodoxin reductase (adr) and adrenodoxin (adx4-108), vd3 was converted by cyp109e1 into several products. furthermore, a whole-cell system in b. megaterium ms941 was established. the new system showed a conversion of 95% after 24h. by nmr analysis it was found that cyp109e1 catalyzes hydroxylation of vd3 at carbons c-24 and c-25, resulting in the f ... | 2017 | 28043840 |
| cd immobilization and reduced tissue cd accumulation of rice (oryza sativa wuyun-23) in the presence of heavy metal-resistant bacteria. | two metal-resistant bacillus megaterium h3 and neorhizobium huautlense t1-17 were investigated for their immobilization of cd in solution and tissue cd accumulation of rice (oryza sativa wuyun-23) in the cd-contaminated soil. strains h3 and t1-17 decreased 79-96% of water-soluble cd in solution and increased grain biomass in the high cd-contaminated soil. inoculation with h3 and t1-17 significantly decreased the root (ranging from 25% to 58%), above-ground tissue (ranging from 13% to 34%), and p ... | 2017 | 28011421 |
| highly regioselective hydroxylation of polydatin, a resveratrol glucoside, for one-step synthesis of astringin, a piceatannol glucoside, by p450 bm3. | enzymatic conversion of natural glycosides to their corresponding hydroxylated products using cytochromes p450 has significant advantages over synthetic chemistry and even enzyme-catalyzed glycosylation of chemicals. at present, the basic strategy for making glycosides of stilbenoid compounds is to use the glycosylation activity of enzymes, such as glycosyltransferases. here, an efficient synthesis of a valuable (e)-astringin, a piceatannol glucoside, was developed using cyp102a1 via the highly ... | 2017 | 28010771 |
| biotransformation of prednisone and dexamethasone by cytochrome p450 based systems - identification of new potential drug candidates. | prednisone and dexamethasone are synthetic glucocorticoids widely used as anti-inflammatory and immunosuppressive drugs. since their hydroxylated derivatives could serve as novel potential drug candidates, our aim was to investigate their biotransformation by the steroid hydroxylase cyp106a2 from bacillus megaterium atcc13368. in vitro we were able to demonstrate highly selective 15β-hydroxylation of the steroids with a reconstituted cyp106a2 system. the reactions were thoroughly characterized, ... | 2017 | 27988215 |
| improving free radical scavenging activity of soy isoflavone glycosides daidzin and genistin by 3'-hydroxylation using recombinant escherichia coli. | the present study describes the biotransformation of a commercially available crude extract of soy isoflavones, which contained significant amounts of the soy isoflavone glycosides daidzin and genistin, by recombinant escherichia coli expressing tyrosinase from bacillus megaterium. two major products were isolated from the biotransformation and identified as 3'-hydroxydaidzin and 3'-hydroxygenistin, respectively, based on their mass and nuclear magnetic resonance spectral data. the two 3'-hydrox ... | 2016 | 27983702 |
| chitosan hydrogel microspheres: an effective covalent matrix for crosslinking of soluble dextranase to increase stability and recycling efficiency. | dextranase is a unique biocatalyst that has high specificity and stereo-selectivity towards a complex biopolymer known as dextran. dextranase has wide industrial application, but most of the time harsh environmental conditions adversely affect the functionality and stability of the enzyme. to overcome this issue, a covalent cross-linking immobilization method was adapted in the current study utilizing a nontoxic and biocompatible matrix known as chitosan. chitosan hydrogel microspheres were synt ... | 2017 | 27904965 |
| in situ affinity purification of his-tagged protein a from bacillus megaterium cultivation using recyclable superparamagnetic iron oxide nanoparticles. | this paper discusses the use of recyclable functionalized nanoparticles for an improved downstream processing of recombinant products. the gram-positive bacterium bacillus megaterium was used to secrete recombinant protein a fused to a histidine tag into the culture supernatant in shaker flasks. superparamagnetic iron oxide nanoparticles functionalized with 3-glycidoxypropyl-trimethoxysilane-coupled-nitrilotriacetic-acid groups (gnta-spion) were synthesized and added directly to the growing cult ... | 2017 | 27888121 |
| a novel c-type lectin in the black tiger shrimp penaeus monodon functions as a pattern recognition receptor by binding and causing bacterial agglutination. | c-type lectins are pattern recognition proteins that play important roles in innate immunity in invertebrates by mediating the recognition of pathogens. in this study, a novel c-type lectin gene, pmclec, was cloned and characterized from the black tiger shrimp penaeus monodon. the open reading frame of pmclec is 657 bp in length. it encodes a predicted protein of 218 amino acids with a calculated molecular mass and an isoelectric point of 24086 da and 4.67, respectively. sequence analysis of pmc ... | 2017 | 27876622 |
| the exosporium of bacillus megaterium qm b1551 is permeable to the red fluorescence protein of the coral discosoma sp. | bacterial spores spontaneously interact and tightly bind heterologous proteins. a variety of antigens and enzymes have been efficiently displayed on spores of bacillus subtilis, the model system for spore formers. adsorption on b. subtilis spores has then been proposed as a non-recombinant approach for the development of mucosal vaccine/drug delivery vehicles, biocatalysts, bioremediation, and diagnostic tools. we used spores of b. megaterium qm b1551 to evaluate their efficiency as an adsorptio ... | 2016 | 27867376 |
| clinical effects of probiotics containing bacillus species on gingivitis: a pilot randomized controlled trial. | lactobacillus spp. and bifidobacteria are the most frequently used probiotics in oral health research. however, although probiotic effects have been suggested for other genera, such as bacilli, no trials are available to describe the effect of bacilli probiotics on gingivitis in humans. the aim of the present study was to evaluate the clinical effects of a bacilli-containing toothpaste, a mouthrinse and a toothbrush cleaner versus a placebo in patients with generalized gingivitis. | 2016 | 27859252 |
| expression and biochemical characterization of a novel type i pullulanase from bacillus megaterium. | to identify novel pullulanases from microorganisms and to investigate their biochemical characterizations. | 2017 | 27858318 |
| an hd domain phosphohydrolase active site tailored for oxetanocin-a biosynthesis. | hd domain phosphohydrolase enzymes are characterized by a conserved set of histidine and aspartate residues that coordinate an active site metallocenter. despite the important roles these enzymes play in nucleotide metabolism and signal transduction, few have been both biochemically and structurally characterized. here, we present x-ray crystal structures and biochemical characterization of the bacillus megaterium hd domain phosphohydrolase oxsa, involved in the biosynthesis of the antitumor, an ... | 2016 | 27849620 |
| isolation, identification, and optimization of culture conditions of a bioflocculant-producing bacterium bacillus megaterium sp1 and its application in aquaculture wastewater treatment. | a bioflocculant-producing bacterium, bacillus megaterium sp1, was isolated from biofloc in pond water and identified by using both 16s rdna sequencing analysis and a biolog gen iii microstation system. the optimal carbon and nitrogen sources for bacillus megaterium sp1 were 20 g l(-1) of glucose and 0.5 g l(-1) of beef extract at 30°c and ph 7. the bioflocculant produced by strain sp1 under optimal culture conditions was applied into aquaculture wastewater treatment. the removal rates of chemica ... | 2016 | 27840823 |
| identification of a new plasmid-encoded cytochrome p450 cyp107dy1 from bacillus megaterium with a catalytic activity towards mevastatin. | in the current work, we describe the identification and characterization of the first plasmid-encoded p450 (cyp107dy1) from a bacillus species. the recombinant cyp107dy1 exhibits characteristic p450 absolute and reduced co-bound difference spectra. reconstitution with different redox systems revealed the autologous one, consisting of bmcpr and fdx2, as the most effective one. screening of a library of 18 pharmaceutically relevant compounds displayed activity towards mevastatin to produce pravast ... | 2016 | 27816653 |
| structural analysis of macromolecular levan produced by bacillus megaterium gjt321 based on enzymatic method. | extracellular polysaccharide (eps) produced by bacillus megaterium gjt321 was isolated from fermentation broth and further purified by gel filtration chromatography. the molecular weight of eps was estimated as 1946kda by gel permeation chromatography (gpc), so this eps was determined as macromolecular polysaccharide. fourier transform infrared spectrometry (ft-ir) showed that the kind of heterocyclic compound composing eps was furanose. the structural characteristics of eps were investigated by ... | 2016 | 27670726 |
| simultaneous biosynthesis of polyhydroxyalkanoates and extracellular polymeric substance (eps) from crude glycerol from biodiesel production by different bacterial strains. | simultaneous synthesis of polyhydroxyalkanoates (phas) and polyglutamic acid (pga) was investigated in cultures of cupriavidus necator ipt 026, c. necator ipt 027, c. necator ipt 029, and bacillus megaterium incqs 425 strains in a medium containing 2.0 % sucrose or crude glycerol from biodiesel (cgb), in an orbital shaker (35 °c, 180 rpm, 72 h). all the strains tested simultaneously produced pha and pga in a medium containing cgb. the c. necator ipt026 culture provided higher molecular mass pha ... | 2016 | 27357823 |
| chemistry and bioactivity of briaranes from the south china sea gorgonian dichotella gemmacea. | seven new briarane diterpenoids, gemmacolides az-bf (1-7), were isolated together with eight known analogues (8-15) from the south china gorgonian dichotella gemmacea. their structures were elucidated based on detailed spectroscopic analysis and a comparison with reported data. in an in vitro bioassay, these compounds exhibited different levels of growth inhibition activity against a549 and mg63 cells, giving continuous evidences about the biological contribution of functional groups at c-2, c-1 ... | 2016 | 27801821 |
| antimicrobial activity and chemical composition of three essential oils extracted from mediterranean aromatic plants. | there is a growing interest in essential oils (eos) as possible alternatives for traditional chemical pesticides. this study was carried out to characterize the chemical composition of the three eos extracted from verbena officinalis, majorana hortensis, and salvia officinalis using gas chromatography (gc) and gc-mass spectrometry (gc-ms) and to evaluate in vitro their efficacy against some phyto or human pathogens. the antifungal activity was investigated against colletotrichum acutatum and bot ... | 2016 | 27792456 |
| enhancement of simultaneous gold and copper recovery from discarded mobile phone pcbs using bacillus megaterium: rsm based optimization of effective factors and evaluation of their interactions. | bioleaching of au from mobile phone printed circuit boards (mppcbs) was studied, using bacillus megaterium which is a cyanogenic bacterium. to maximize au extraction, initial ph, pulp density, and glycine concentration were optimized via response surface methodology (rsm). bioleaching of cu, an important inhibitor on au recovery, was also examined. to maximize au recovery, the optimal condition suggested by the models was initial ph of 10, pulp density of 8.13g/l, and glycine concentration of 10 ... | 2016 | 27264460 |
| inoculation with endophytic bacillus megaterium h3 increases cd phytostabilization and alleviates cd toxicity to hybrid pennisetum in cd-contaminated aquatic environments. | a hydroponic culture experiment was performed to investigate the effects of endophytic bacillus megaterium h3 on the plant biomass, cd accumulation and tolerance of hybrid pennisetum, and the mechanisms involved in the different levels of cd-contaminated aquatic environments. strain h3 significantly increased the plant growth (ranging from 13 to 71 %) and total cd uptake (ranging from 41 to 160 %) but decreased cd translocation factors of hybrid pennisetum treated with 0-20 μm cd compared with t ... | 2017 | 27783244 |
| effects of n-/c-terminal extra tags on the optimal reaction conditions, activity, and quaternary structure of bacillus thuringiensis glucose 1-dehydrogenase. | glucose dehydrogenase (gdh) is an oxidoreductase enzyme and is used as a biocatalyst to regenerate nad(p)h in reductase-mediated chiral synthesis reactions. in this study, the glucose 1-dehydrogenase b gene (gdhb) was cloned from bacillus thuringiensis subsp. kurstaki, and wild-type (gdh-bt(wt)) and his-tagged (gdh-bt(n-his), gdh-bt(c-his)) enzymes were produced in escherichia coli bl21 (de3). all enzymes were produced in the soluble forms from e. coli. gdh-bt(wt) and gdh-bt(n-his) showed high s ... | 2016 | 27363470 |
| integral use of amaranth starch to obtain cyclodextrin glycosyltransferase, by bacillus megaterium, to produce β-cyclodextrin. | cyclodextrin glycosyltransferase (cgtase) is an enzyme that produces cyclodextrins (cds) from starch and related carbohydrates, producing a mixture of α-, β-, and γ-cds in different amounts. cgtase production, mainly by bacillus sp., depends on fermentation conditions such as ph, temperature, concentration of nutrients, carbon and nitrogen sources, among others. bacillus megaterium cgtase produces those three types of cds, however, β-cd should prevail. although, waxy corn starch (cs) is used ind ... | 2016 | 27721811 |
| changes in bacillus spore small molecules, rrna, germination, and outgrowth after extended sublethal exposure to various temperatures: evidence that protein synthesis is not essential for spore germination. | rrnas of dormant spores of bacillus subtilis were >95% degraded during extended incubation at 50°c, as reported previously (e. segev, y. smith, and s. ben-yehuda, cell 148:139-114, 2012, doi:http://dx.doi.org/10.1016/j.cell.2011.11.059), and this was also true of spores of bacillus megaterium incubation of spores of these two species for ∼20 h at 75 to 80°c also resulted in the degradation of all or the great majority of the 23s and 16s rrnas, although this rrna degradation was slower than nonen ... | 2016 | 27645383 |
| regioselective c-h hydroxylation of omeprazole sulfide by bacillus megaterium cyp102a1 to produce a human metabolite. | to find a simple enzymatic strategy for the efficient synthesis of the expensive 5'-hydroxyomeprazole sulfide, a recently identified minor human metabolite, from omeprazole sulfide, which is an inexpensive substrate. | 2017 | 27640009 |
| identification of lipopeptides in bacillus megaterium by two-step ultrafiltration and lc-esi-ms/ms. | a two-step ultrafiltration and liquid chromatography-electronic spray ionization-tandem mass spectrometry (lc-esi-ms/ms) were applied to investigate the nature of lipopeptides in the methanol extract harvested from marine bacillus megaterium. the structure of lipopeptide homologs were characterized by using collision-induced dissociation mass spectrometry analysis. collectively, two kinds of linear fengycin a and b with a double bond were characterized for the first time besides the popular cycl ... | 2016 | 27639854 |
| soil bacteria as sources of virulence signal providers promoting plant infection by phytophthora pathogens. | phytophthora species are known as "plant destroyers" capable of initiating single zoospore infection in the presence of a quorum of chemical signals from the same or closely related species of oomycetes. since the natural oomycete population is too low to reach a quorum necessary to initiate a disease epidemic, creation of the quorum is reliant on alternate sources. here, we show that a soil bacterial isolate, bacillus megaterium sb5, promotes plant infection by phytophthora species. in the pres ... | 2016 | 27616267 |
| cytochromes p450: history, classes, catalytic mechanism, and industrial application. | cytochromes p450, a family of heme-containing monooxygenases that catalyze a diverse range of oxidative reactions, are so-called due to their maximum absorbance at 450nm, ie, "pigment-450nm," when bound to carbon monoxide. they have appeal both academically and commercially due to their high degree of regio- and stereoselectivity, for example, in the area of active pharmaceutical ingredient synthesis. despite this potential, they often exhibit poor stability, low turnover numbers and typically r ... | 2016 | 27567486 |
| immobilization of iron- and manganese-oxidizing bacteria with a biofilm-forming bacterium for the effective removal of iron and manganese from groundwater. | in this study, three bacteria with high fe- and mn-oxidizing capabilities were isolated from groundwater well sludge and identified as acinetobacter sp., bacillus megaterium and sphingobacterium sp. the maximum removal ratios of fe and mn (99.75% and 96.69%) were obtained by an optimal combination of the bacteria at a temperature of 20.15°c, ph 7.09 and an inoculum size of 2.08%. four lab-scale biofilters were tested in parallel for the removal of iron and manganese ions from groundwater. the re ... | 2016 | 27566515 |
| cumulative role of bioinoculants on growth, antioxidant potential and artemisinin content in artemisia annua l. under organic field conditions. | artemisia annua l. is mostly known for a bioactive metabolite, artemisinin, an effective sesquiterpene lactone used against malaria without any reputed cases of resistance. in this experiment, bioinoculants viz., streptomyces sp. mtn14, bacillus megaterium mtn2rp and trichoderma harzianum thu were applied as growth promoting substances to exploit full genetic potential of crops in terms of growth, yield, nutrient uptake and particularly artemisinin content. further, multi-use of the bioinoculant ... | 2016 | 27565777 |
| fusion to hydrophobin hfbi improves the catalytic performance of a cytochrome p450 system. | cytochrome p450 monooxygenases (p450) are heme-containing enzymes that oxidize a broad range of substrates in the presence of molecular oxygen and nad(p)h. for their activity, most p450s rely on one or two redox proteins responsible for the transfer of electrons from the cofactor nad(p)h to the heme. one of the challenges when using p450s in vitro, especially when non-physiological redox proteins are applied, is the inefficient transfer of electrons between the individual proteins resulting in n ... | 2016 | 27458582 |
| structural basis of the substrate range and enantioselectivity of two (s)-selective ω-transaminases. | ω-transaminases are enzymes that can introduce an amino group in industrially interesting compounds. we determined crystal structures of two (s)-selective ω-transaminases, one from arthrobacter sp. (ars-ωta) and one from bacillus megaterium (bm-ωta), which have 95% identical sequences but somewhat different activity profiles. substrate profiling measurements using a range of (r)- and (s)-substrates showed that both enzymes have a preference for substrates with large, flat cyclic side groups, for ... | 2016 | 27428867 |
| cyp3a specifically catalyzes 1β-hydroxylation of deoxycholic acid: characterization and enzymatic synthesis of a potential novel urinary biomarker for cyp3a activity. | the endogenous bile acid metabolite 1β-hydroxy-deoxycholic acid (1β-oh-dca) excreted in human urine may be used as a sensitive cyp3a biomarker in drug development reflecting in vivo cyp3a activity. an efficient and stereospecific enzymatic synthesis of 1β-oh-dca was developed using a bacillus megaterium (bm3) cytochrome p450 (p450) mutant, and its structure was confirmed by nuclear magnetic resonance (nmr) spectroscopy. a [(2)h4]-labeled analog of 1β-oh-dca was also prepared. the major hydroxyla ... | 2016 | 27402728 |
| production of ω-hydroxy palmitic acid using cyp153a35 and comparison of cytochrome p450 electron transfer system in vivo. | bacterial cytochrome p450 enzymes in cytochrome p450 (cyp)153 family were recently reported as fatty acid ω-hydroxylase. among them, cyp153as from marinobacter aquaeolei vt8 (cyp153a33), alcanivorax borkumensis sk2 (cyp153a13), and gordonia alkanivorans (cyp153a35) were selected, and their specific activities and product yields of ω-hydroxy palmitic acid based on whole cell reactions toward palmitic acid were compared. using camab as redox partner, cyp153a35 and cyp153a13 showed the highest prod ... | 2016 | 27344594 |
| studies on the production of shikimic acid using the arok knockout strain of bacillus megaterium. | shikimic acid has various pharmaceutical and industrial applications. it is the sole chemical building block for the antiviral drug oseltamivir (tamiflu(®)) and one of the potent pharmaceutical intermediates with three chiral centres. here we report a modified strain of bacillus megaterium with arok (shikimate kinase) knock out to block the aromatic biosynthetic pathway downstream of shikimic acid. homologous recombination based gene disruption approach was used for generating arok knock out mut ... | 2016 | 27339308 |
| antioxidative, antibacterial, and food functional properties of the half-fin anchovy hydrolysates-glucose conjugates formed via maillard reaction. | the antioxidative, antibacterial, and food functional properties of the half-fin anchovy hydrolysates (hahp)-glucose conjugates formed by maillard reaction (mr) were investigated, respectively. results of sugar and amino acid contents loss rates, browning index, and molecular weight distribution indicated that the initial ph of hahp played an important role in the process of mr between hahp and glucose. hahp-glucose maillard reaction products (hahp-g mrps) demonstrated enhanced antioxidative act ... | 2016 | 27331806 |
| effect of annealing temperature on antimicrobial and structural properties of bio-synthesized zinc oxide nanoparticles using flower extract of anchusa italica. | the use of nontoxic biological compounds in the synthesis of nanomaterials is an economic and eco-friendly approach. the present work was undertaken to develop zinc oxide nanoparticles (zno-nps) by a green method using simple precursor from the solution consisting of zinc acetate and the flower extract of anchusa italica (a. italica). effect of annealing temperature on structural and antimicrobial properties was investigated. the crystalline structure of zno-nps was shown using x-ray diffraction ... | 2016 | 27318600 |
| extraction and analysis of peptidoglycan cell wall precursors. | extraction and analysis by lc-ms of peptidoglycan precursors represent a valuable method to study antibiotic mode of action and resistance in bacteria. here, we describe how to apply this method for: (1) testing the action of different classes of antibiotics inhibiting cell wall biosynthesis in bacillus megaterium; (2) studying the mechanism of self-resistance in mycelial actinomycetes producing glycopeptide antibiotics. | 2016 | 27311671 |
| bacillus megaterium sf185 induces stress pathways and affects the cell cycle distribution of human intestinal epithelial cells. | the interaction between the enteric microbiota and intestinal cells often involves signal molecules that affect both microbial behaviour and host responses. examples of such signal molecules are the molecules secreted by bacteria that induce quorum sensing mechanisms in the producing microorganism and signal transduction pathways in the host cells. the pentapeptide competence and sporulation factor (csf) of bacillus subtilis is a well characterized quorum sensing factor that controls competence ... | 2016 | 27291405 |
| surface display of bacterial tyrosinase on spores of bacillus subtilis using cote as an anchor protein. | tyrosinases, copper-containing monooxygenases, are widely used enzymes for industrial, medical, and environmental applications. we report the first functional surface display of bacillus megaterium tyrosinase on bacillus subtilis spores using cote as an anchor protein. flow cytometry was used to verify surface expression of tyrosinase on the purified spores. moreover, tyrosinase activity of the displayed enzyme on b. subtilis spores was monitored in the presence of l-tyrosine (substrate) and cus ... | 2016 | 27281458 |
| effect of mutation and substrate binding on the stability of cytochrome p450bm3 variants. | cytochrome p450bm3 is a heme-containing enzyme from bacillus megaterium that exhibits high monooxygenase activity and has a self-sufficient electron transfer system in the full-length enzyme. its potential synthetic applications drive protein engineering efforts to produce variants capable of oxidizing nonnative substrates such as pharmaceuticals and aromatic pollutants. however, promiscuous p450bm3 mutants often exhibit lower stability, thereby hindering their industrial application. this study ... | 2016 | 27267136 |
| semirational protein engineering of cyp153am.aq. -cprbm3 for efficient terminal hydroxylation of short- to long-chain fatty acids. | the regioselective terminal hydroxylation of alkanes and fatty acids is of great interest in a variety of industrial applications, such as in cosmetics, in fine chemicals, and in the fragrance industry. the chemically challenging activation and oxidation of non-activated c-h bonds can be achieved with cytochrome p450 enzymes. cyp153am.aq. -cprbm3 is an artificial fusion construct consisting of the heme domain from marinobacter aquaeolei and the reductase domain of cyp102a1 from bacillus megateri ... | 2016 | 27251775 |
| a novel nadph-dependent flavoprotein reductase from bacillus megaterium acts as an efficient cytochrome p450 reductase. | cytochromes p450 (p450s) require electron transfer partners to catalyze substrate conversions. with regard to biotechnological approaches, the elucidation of novel electron transfer proteins is of special interest, as they can influence the enzymatic activity and specificity of the p450s. in the current work we present the identification and characterization of a novel soluble nadph-dependent diflavin reductase from bacillus megaterium with activity towards a bacterial (cyp106a1) and a microsoma ... | 2016 | 27238232 |
| identification of bacillus megaterium and microbacterium liquefaciens genes involved in metal resistance and metal removal. | bacillus megaterium mnsh1-9k-1 and microbacterium liquefaciens mnsh2-phgii-2, 2 nickel- and vanadium-resistant bacteria from mine tailings located in guanajuato, mexico, are shown to have the ability to remove 33.1% and 17.8% of ni, respectively, and 50.8% and 14.0% of v, respectively, from spent petrochemical catalysts containing 428 ± 30 mg·kg(-1) ni and 2165 ± 77 mg·kg(-1) v. in these strains, several ni resistance determinants were detected by conventional pcr. the ncca (nickel-cobalt-cadmiu ... | 2016 | 27210016 |
| alleviating salt stress in tomato seedlings using arthrobacter and bacillus megaterium isolated from the rhizosphere of wild plants grown on saline-alkaline lands. | salt-induced soil degradation is common in farmlands and limits the growth and development of numerous crop plants in the world. in this study, we isolated salt-tolerant bacteria from the rhizosphere of tamarix chinensis, suaeda salsa and zoysia sinica, which are common wild plants grown on a saline-alkaline land, to test these bacteria's efficiency in alleviating salt stress in tomato plants. we screened out seven strains (tf1-7) that are efficient in reducing salt stress in tomato seedlings. t ... | 2016 | 27196364 |
| draft genome sequence of bacillus megaterium bhg1.1, a strain isolated from bar-headed goose (anser indicus) feces on the qinghai-tibet plateau. | bacillus megaterium is a soil-inhabiting gram-positive bacterium that is routinely used in industrial applications for recombinant protein production and bioremediation. studies involving bacillus megaterium isolated from waterfowl are scarce. here, we report a 6.26-mbp draft genome sequence of bacillus megaterium bhg1.1, which was isolated from feces of a bar-headed goose. | 2016 | 27174262 |
| a bacillus megaterium system for the production of recombinant proteins and protein complexes. | for many years the gram-positive bacterium bacillus megaterium has been used for the production and secretion of recombinant proteins. for this purpose it was systematically optimized. plasmids with different inducible promoter systems, with different compatible origins, with small tags for protein purification and with various specific signals for protein secretion were combined with genetically improved host strains. finally, the development of appropriate cultivation conditions for the produc ... | 2016 | 27165321 |
| effects of different heat treatments on lysozyme quantity and antimicrobial activity of jenny milk. | thermal treatments are used to improve milk microbial safety, shelf life, and biological activity of some of its components. however, thermal treatments can reduce the nutritional quality of milk, affecting the molecular structure of milk proteins, such as lysozyme, which is a very important milk component due to its antimicrobial effect against gram-positive bacteria. jenny milk is characterized by high lysozyme content. for this reason, in the last few years, it has been used as an antimicrobi ... | 2016 | 27157571 |
| novel bioassay using bacillus megaterium to detect tetracycline in milk. | tetracyclines are used for the prevention and control of dairy cattle diseases. residues of these drugs can be excreted into milk. thus, the aim of this study was to develop a microbiological method using bacillus megaterium to detect tetracyclines (chlortetracycline, oxytetracycline and tetracycline) in milk. in order to approximate the limits of detection of the bioassay to the maximum residue limit (100μg/l) for milk tetracycline, different concentrations of chloramphenicol (0, 1000, 1500 and ... | 2017 | 27131738 |
| expression analysis of ni- and v-associated resistance genes in a bacillus megaterium strain isolated from a mining site. | bacillus megaterium strain mnsh1-9k-1 was isolated from a mining site in guanajuato, mexico. this b. megaterium strain presented the ability to remove ni and v from a spent catalyst. also, its associated metal resistance genes ncca, hant, van2, and smtab were previously identified by a pcr approach. the present study reports for the first time, in b. megaterium, the changes in the expression of the genes ncca (ni-co-cd resistance); hant (high-affinity nickel transporter); smtab, a metal-binding ... | 2016 | 27107759 |
| complete genome sequence of bacillus megaterium bacteriophage eldridge. | in this study the complete genome sequence of the unique bacteriophage eldridge, isolated from soil using italic! bacillus megateriumas the host organism, was determined. eldridge is a myovirus with a genome consisting of 242 genes and is unique when compared to phage sequences in genbank. | 2016 | 27103735 |
| [isolation of microorganisms producing enzyme capable of degrading tobacco straw and nicotine]. | the aim of this study was to screen tobacco straw and nicotine degrading microorganism. | 2015 | 27101696 |
| influence of antibiotic adsorption on biocidal activities of silver nanoparticles. | excessive use of antibiotics has posed two major challenges in public healthcare. one of them is associated with the development of multi-drug resistance while the other one is linked to side effects. in the present investigation, the authors report an innovative approach to tackle the challenges of multi-drug resistance and acute toxicity of antibiotics by using antibiotics adsorbed metal nanoparticles. monodisperse silver nanoparticles (snps) have been synthesised by two-step process. in the f ... | 2016 | 27074856 |
| synthesis, antimicrobial, and antiproliferative activities of substituted phenylfuranylnicotinamidines. | this research work deals with the design and synthesis of a series of substituted phenylfuranylnicotinamidines 4a-i. facile preparation of the target compounds was achieved by suzuki coupling-based synthesis of the nitrile precursors 3a-i, followed by their conversion to the corresponding nicotinamidines 4a-i utilizing lin(tms)2. the antimicrobial activities of the newly synthesized nicotinamidine derivatives were evaluated against the gram-negative bacterial strains escherichia coli and pseudom ... | 2016 | 27042005 |
| functional and structural characterization of two bacillus megaterium nitroreductases biotransforming the herbicide mesotrione. | mesotrione is a selective herbicide belonging to the triketone family, commonly used on maize cultures since 2003. a mesotrione-transforming bacillus megaterium mes11 strain isolated from an agricultural soil was used as a model to identify the key enzymes initiating the biotransformation of this herbicide. two enzymes (called nfra1 and nfra2/ycnd) were identified, and functionally and structurally characterized. both belong to the nfsa frp family of the nitro-fmn reductase superfamily (type i o ... | 2016 | 27005432 |
| genetic engineering of bacillus megaterium for high-yield production of the major teleost progestogens 17α,20β-di- and 17α,20β,21α-trihydroxy-4-pregnen-3-one. | 17α,20β-dihydroxy-4-pregnen-3-one (17α,20βdioh-p) and 17α,20β,21α-trihydroxy-4-pregnen-3-one (20βoh-rss) are the critical hormones required for oocyte maturation in fish. we utilized b. megaterium's endogenous 20β-hydroxysteroid dehydrogenase (20βhsd) for the efficient production of both progestogens after genetically modifying the microorganism to reduce side-product formation. first, the gene encoding the autologous cytochrome p450 cyp106a1 was deleted, resulting in a strain devoid of any ster ... | 2016 | 26976492 |
| the regulatory mechanism underlying light-inducible production of carotenoids in nonphototrophic bacteria. | light is a ubiquitous environmental factor serving as an energy source and external stimulus. here, i review the conserved molecular mechanism of light-inducible production of carotenoids in three nonphototrophic bacteria: streptomyces coelicolor a3(2), thermus thermophilus hb27, and bacillus megaterium qm b1551. a merr family transcriptional regulator, litr, commonly plays a central role in their light-inducible carotenoid production. genetic and biochemical studies on litr proteins revealed a ... | 2016 | 26967471 |
| peptide-mediated specific immobilization of catalytically active cytochrome p450 bm3 variant. | cytochrome p450 bm3 (cyp102a1) from bacillus megaterium is an interesting target for biotechnological applications, because of its vast substrate variety combined with high p450 monooxygenase activity. the low stability in vitro could be overcome by immobilization on surfaces. here we describe a novel method for immobilization on metal surfaces by using selectively binding peptides. a p450 bm3 triple mutant (3m-p450bm3: a74g, f87v, l188q) was purified as protein thioester and ligated to indium t ... | 2016 | 26967204 |
| evaluation of novel inducible promoter/repressor systems for recombinant protein expression in lactobacillus plantarum. | engineering lactic acid bacteria (lab) is of growing importance for food and feed industry as well as for in vivo vaccination or the production of recombinant proteins in food grade organisms. often, expression of a transgene is only desired at a certain time point or period, e.g. to minimize the metabolic burden for the host cell or to control the expression time span. for this purpose, inducible expression systems are preferred, though cost and availability of the inducing agent must be feasib ... | 2016 | 26966093 |
| metabolic engineering of escherichia coli for the production of hydroxy fatty acids from glucose. | hydroxy fatty acids (hfas) are valuable chemicals for a broad variety of applications. however, commercial production of hfas has not been established so far due to the lack of low cost routes for their synthesis. although the microbial transformation pathway of hfas was extensively studied decades ago, these attempts mainly focused on converting fatty acids or vegetable oils to their hydroxyl counterparts. the use of a wider range of feedstocks to produce hfas would reduce the dependence on oil ... | 2016 | 26956722 |
| expression, characterization and mutagenesis of a novel glutamate decarboxylase from bacillus megaterium. | to search for a novel glutamate decarboxylase (gad) with an optimum ph towards near-neutrality in order to improve production of gamma-aminobutyric acid (gaba) in recombinant hosts. | 2016 | 26956237 |
| human cyp27a1 catalyzes hydroxylation of β-sitosterol and ergosterol. | β-sitosterol and ergosterol are the equivalents of cholesterol in plants and fungi, respectively, and common sterols in the human diet. in the current work, both were identified as novel cyp27a1 substrates by in vitro experiments applying purified human cyp27a1 and its redox partners adrenodoxin (adx) and adrenodoxin reductase (adr). a bacillus megaterium based biocatalyst recombinantly expressing the same proteins was utilized for the conversion of the substrates to obtain sufficient amounts of ... | 2016 | 26891232 |
| crystal structure of cyp106a2 in substrate-free and substrate-bound form. | cyp106a2 from bacillus megaterium atcc 13368 is known as a bacterial steroid hydroxylase that is also capable of hydroxylating a variety of terpenoids. to analyze the substrate specificity of this enzyme further, different resin acids of the abietane and pimarane types were tested with regard to binding and conversion. product formation could be shown for all tested substrates. spectroscopic studies revealed type i binding spectra for isopimaric acid, but dehydroabietic acid did not induce a hig ... | 2016 | 26864272 |
| relationship between in vitro characterization and comparative efficacy of plant growth-promoting rhizobacteria for improving cucumber salt tolerance. | phosphate solubilization, 1-aminocyclopropane-1-carboxylic acid (acc)-deaminase activity and production of siderophores and indole acetic acid (iaa) are well-known traits of plant growth-promoting rhizobacteria (pgpr). here we investigated the expression of these traits as affected by salinity for three pgpr strains (pseudomonas fluorescens, bacillus megaterium and variovorax paradoxus) at two salinity levels [2 and 5 % nacl (w/v)]. among the three strains, growth of b. megaterium was the least ... | 2016 | 26860842 |
| an in vitro attempt for controlling severe phytopathogens and human pathogens using essential oils from mediterranean plants of genus schinus. | growing concerns about food safety and environmental protection enhanced the need for new and safe plant disease control strategies. the chemical composition of the three essential oils (eos) extracted from leaves and fruits of schinus terebinthifolius and leaves of schinus molle, growing in tunisia, was studied by gc and gc-ms. in all, 12 compounds were identified. the oils were mainly composed of terpene compounds. α-pinene, α-phellandrene, and d-limonene were the major constituents. the aim o ... | 2016 | 26836214 |
| structural and biochemical characterization of a copper-binding mutant of the organomercurial lyase merb: insight into the key role of the active site aspartic acid in hg-carbon bond cleavage and metal binding specificity. | in bacterial resistance to mercury, the organomercurial lyase (merb) plays a key role in the detoxification pathway through its ability to cleave hg-carbon bonds. two cysteines (c96 and c159; escherichia coli merb numbering) and an aspartic acid (d99) have been identified as the key catalytic residues, and these three residues are conserved in all but four known merb variants, where the aspartic acid is replaced with a serine. to understand the role of the active site serine, we characterized th ... | 2016 | 26820485 |
| isolation and identification of bacillus megaterium yb3 from an effluent contaminated site efficiently degrades pyrene. | industrial effluents contaminated sites may serve as repositories of ecologically adapted efficient pyrene degrading bacteria. in the present study, six bacterial isolates from industrial effluents were purified using serial enrichment technique and their pyrene degrading potential on pyrene supplemented mineral salt medium was assessed. 16s rrna sequence analysis showed that they belong to four bacterial genera, namely acinetobacter, bacillus, microbacterium, and ochrobactrum. among these isola ... | 2016 | 26755240 |
| the role of cytochrome p450 bm3 phenylalanine-87 and threonine-268 in binding organic hydroperoxides. | cytochrome p450 (p450) bm3, from bacillus megaterium, catalyzes a wide range of chemical reactions and is routinely used as a model system to study mammalian p450 reactions and structure. | 2016 | 26723172 |
| dual action of chromium-reducing and nitrogen-fixing bacillus megaterium-asnf3 for improved agro-rehabilitation of chromium-stressed soils. | we conducted a study for enhanced biological rehabilitation of chromium-contaminated soils using a chromium-reducing and nitrogen-fixing bacterial species (bacillus megaterium-asnf3). the bacterial species was isolated from a chromium-rich land area, characterized, and employed under optimum conditions for the treatment of artificially prepared chromium-rich soil. the bacterium reduced cr(vi) up to 86 % in a 60-day trial of incubation in the soil bioreactor. the nitrogenase activity of the bacte ... | 2016 | 28330201 |
| synthesis and antimicrobial evaluation of cyanopyridinyl tetrahydronaphthalene derivatives. | a novel series of cyanopyridinyl tetrahydronaphthalene incorporated with different heterocycles were synthesized. the key compounds 2a,b were condensed with chloroacetone and ethyl chloroacetate to give 3a,b and 4a,b, respectively. also condensation of 4a,b with hydrazine hydrate gave the corresponding hydrazide 5a,b. reaction of 5b with different isothiocyanates gave the corresponding thiosemicarbazide derivatives 6a-c. also, condensation of 5a with chloroacetic acid, methyl iodide and/or aceti ... | 2015 | 26642656 |
| expression of human cyp27a1 in b. megaterium for the efficient hydroxylation of cholesterol, vitamin d3 and 7-dehydrocholesterol. | in the current work the ability of bacillus megaterium to take up hydrophobic substrates and efficiently express eukaryotic membrane proteins was utilized for establishing a cyp27a1-based biocatalyst. the human mitochondrial cytochrome p450cyp27a1 was co-expressed with its redox partners adrenodoxin reductase (adr) and adrenodoxin (adx). cyp27a1 could be localized at the cell's polyhydroxybutyrate (phb) granules, carbon storage serving organelle-like vesicles that can take up cholesterol, result ... | 2016 | 26638999 |
| increased plant growth and copper uptake of host and non-host plants by metal-resistant and plant growth-promoting endophytic bacteria. | the effects of inoculation with two metal-resistant and plant growth-promoting endophytic bacteria (burkholderia sp. gl12 and bacillus megaterium jl35) were evaluated on the plant growth and cu uptake in their host elsholtzia splendens and non-host brassica napus plants grown in natural cu-contaminated soil. the two strains showed a high level of acc deaminase activities. in pot experiments, inoculation with strain gl12 significantly increased root and above-ground tissue dry weights of both pla ... | 2016 | 26587767 |
| complementary effects of host defense peptides piscidin 1 and piscidin 3 on dna and lipid membranes: biophysical insights into contrasting biological activities. | piscidins were the first antimicrobial peptides discovered in the mast cells of vertebrates. while two family members, piscidin 1 (p1) and piscidin 3 (p3), have highly similar sequences and α-helical structures when bound to model membranes, p1 generally exhibits stronger antimicrobial and hemolytic activity than p3 for reasons that remain elusive. in this study, we combine activity assays and biophysical methods to investigate the mechanisms underlying the cellular function and differing biolog ... | 2015 | 26569483 |
| comparative evaluation of the antimicrobial activity of 19 essential oils. | in our research on natural compounds efficient against human pathogen or opportunist microorganisms contracted by food or water, the antimicrobial activity of 19 essential oils (eos) was investigated against 11 bacterial species (6 gram positive, 5 gram negative) and 7 fungal species (2 dermatophytes, 1 mould, 4 yeasts) using microdilution assays. five essential oils were obtained from tunisian plants (eotun): artemisia herba-alba asso, juniperus phoenicea l., rosmarinus officinalis l., ruta gra ... | 2016 | 26566647 |
| synthesis and evaluation of in vitro dna/protein binding affinity, antimicrobial, antioxidant and antitumor activity of mononuclear ru(ii) mixed polypyridyl complexes. | the four novel ru(ii) complexes [ru(phen)2mafip](2+) (1) [mafip = 2-(5-(methylacetate)furan-2-yl)-1 h-imidazo[4,5-f] [1, 10]phenanthroline, phen = 1,10-phenanthroline], [ru(bpy)2mafip](2+) (2) (bpy = 2,2'-bipyridine) and [ru(dmb)2mafip](2+) (3) (dmb = 4,4'-dimethyl-2,2'-bipyridine) and [ru(hdpa)2mafip](2+) (4) (hdpa = 2,2-dipyridylamine) have been synthesized and fully characterized via elemental analysis, nmr spectroscopy, ei-ms and ft-ir spectroscopy. in addition, the dna-binding behaviors of ... | 2016 | 26555289 |
| bio-grout based on microbially induced sand solidification by means of asparaginase activity. | bio-grout, a new ground improvement method, has been recently developed to improve the mechanical properties, decrease the permeability of porous materials, reinforce or repair cementitious materials and modify the properties of soil or sand. bio-grout production depends on microbially induced calcite precipitation (micp), which is driven mainly by an enzyme, urease. however, urease-based micp process produces excessive ammonia, in addition to secondary pollution generated by urea that is used a ... | 2015 | 26525435 |
| a stereoselective esterase from bacillus megaterium: purification, gene cloning, expression and catalytic properties. | esterases (ec 3.1.1.x) have been used as biocatalysts due to their good stability, high chemo-, regio- and stereoselectivity. in our previous studies, bacillus megaterium wz009 harboring esterase displayed the unique capability to convert (s)-4-chloro-3-hydroxyethylbutyrate (chbe) in the racemate to (s)-3-hydroxy-γ-butyrolactone (hl) through stereoselective hydrolysis, dechlorination, and lactonization. the remaining (r)-chbe and formed (s)-hl could be obtained in a one-pot enzymatic reaction. a ... | 2015 | 26518366 |
| structure-function analysis of the bacillus megaterium gerud spore germinant receptor protein. | germination of bacillus spores is triggered by the interaction of germinant molecules with specialized receptor proteins localized to the spore inner membrane. germinant receptors (grs) are comprised typically of three interacting protein subunits, each of which is essential for receptor function. at least some grs appear to have a fourth component, referred to as a d-subunit protein. a number of d-subunit proteins were shown previously to be capable of modulating the activity of associated grs. ... | 2015 | 26511952 |
| using tyrosinase as a monophenol monooxygenase: a combined strategy for effective inhibition of melanin formation. | tyrosinase is a binuclear copper-containing metalloprotein that leads the fast and regio-selective o-hydroxylation of monophenols to o-diphenols. however, the subsequent second oxidation to produce o-quinones, i.e., melanin precursors, from the o-diphenols has restricted its use to the production of functional o-diphenol derivatives. herein, we present a combined strategy for the effective inhibition of melanin formation in tyrosinase reaction, which allows the use of tyrosinase as a monophenol ... | 2016 | 26461518 |
| aerobic mercury-resistant bacteria alter mercury speciation and retention in the tagus estuary (portugal). | aerobic mercury-resistant bacteria were isolated from the sediments of two highly mercury-polluted areas of the tagus estuary (barreiro and cala do norte) and one natural reserve area (alcochete) in order to test their capacity to transform mercury. bacterial species were identified using 16s rrna amplification and sequencing techniques and the results indicate the prevalence of bacillus sp. resistance patterns to mercurial compounds were established by the determination of minimal inhibitory co ... | 2016 | 26461264 |
| heterologous coexpression of vitreoscilla hemoglobin and bacillus megaterium glucanase in streptomyces lydicus a02 enhanced its production of antifungal metabolites. | streptomyces lydicus a02 is a novel producer of commercially important polyene macrocyclic antibiotic natamycin and a potential biocontrol agent to several plant fungal diseases, including wilt caused by fusarium oxysporum f. spp. to improve the natamycin production and the antifungal activity of s. lydicus a02, we coexpressed gene vgb encoding vitreoscilla hemoglobin (vhb) and bglc encoding bacillus megaterium l103 glucanase, both under the control of the strong constitutive erme* promoter, in ... | 2015 | 26453475 |
| complete genome sequence of bacillus megaterium siphophage silence. | silence is a newly isolated siphophage that infects bacillus megaterium, a soil bacterium that is used readily in research and commercial applications. a study of b. megaterium phage silence will enhance our knowledge of the diversity of bacillus phages. here, we describe the complete genome sequence and annotated features of silence. | 2015 | 26450722 |
| process limitations of a whole-cell p450 catalyzed reaction using a cyp153a-cpr fusion construct expressed in escherichia coli. | cytochrome p450s are interesting biocatalysts due to their ability to hydroxylate non-activated hydrocarbons in a selective manner. however, to date only a few p450-catalyzed processes have been implemented in industry due to the difficulty of developing economically feasible processes. in this study, we have used the cyp153a heme domain from marinobacter aquaeolei fused to the reductase domain of cyp102a1 from bacillus megaterium (bm3) expressed in escherichia coli. this self-sufficient protein ... | 2016 | 26432459 |
| biosurfactant-enhanced bioremediation of aged polycyclic aromatic hydrocarbons (pahs) in creosote contaminated soil. | the potential for biological treatment of an environment contaminated by complex petrochemical contaminants was evaluated using creosote contaminated soil in ex situ bio-slurry reactors. the efficacy of biosurfactant application and stimulation of in situ biosurfactant production was investigated. the biosurfactant produced was purified and characterised using fourier transform infrared (ftir) spectroscopy. biosurfactant enhanced degradation of pahs was 86.5% (with addition of biosurfactant) and ... | 2016 | 26408261 |
| biodiversity of dominant cultivable endophytic bacteria inhabiting tissues of six different cultivars of maize (zea mays l. ssp. mays) cropped under field conditions. | endophytic bacteria (enb) play a crucial role in plant development. this study was an attempt to isolate and identify dominant cultivable enb inhabiting young seedlings germinated in vitro and leaves of six maize cultivars grown under field conditions at temperate climate zone with culture-dependent approach. we isolated bacteria from field cropped maize only. strains were identified based on 16s rrna gene sequencing. in particular, members of actinobacteria, bacteroidetes, firmicutes and α- and ... | 2015 | 26373177 |
| rock inhabiting potassium solubilizing bacteria from kerala, india: characterization and possibility in chemical k fertilizer substitution. | the role of rock inhabiting bacteria in potassium (k) solubilization from feldspar and their application in crop nutrition through substitution of fertilizer k was explored through the isolation of 36 different bacteria from rocks of a major hill station at ponmudi in thiruvananthapuram, kerala, india. a comprehensive characterization of k solubilization from feldspar was achieved with these isolates which indicated that the k solubilizing efficiency increases with decrease in ph and increase in ... | 2016 | 26350060 |
| poly[(r)-3-hydroxybutyrate] production under different salinity conditions by a novel bacillus megaterium strain. | bacillus megaterium uyuni s29, isolated from the bolivian salt lake uyuni, displays a high capability to produce poly[(r)-3-hydroxybutyrate] (phb) in industrial culture media. in order to analyze the influence of salt on biomass formation and phb production, cultivations at different nacl concentrations were carried out according to the salinity conditions of the habitats of the strain's original isolation. in this preliminary report, the strain showed considerable adaptability to media of diffe ... | 2016 | 26344348 |