| the structure of the cuticle and sheath of the infective juvenile of trichostrongylus colubriformis. | the infective third-stage juvenile of trichostrongylus colubriformis is surrounded by its own cuticle as well as the incompletely moulted cuticle of the second-stage juvenile, which is referred to as the sheath. the sheath comprises an outer epicuticle, an amorphous cortical zone, a fibrous basal zone and an inner electron-dense layer. the basal zone of the sheath consists of three layers of fibres; the fibres are parallel within each layer, but the fibre direction of the middle layer is at an a ... | 1986 | 3799009 |
| [changes in the characteristics of glucocorticoid hormone binding in human peripheral blood lymphocytes during myocardial infarct]. | competitive binding was used to determine the number of binding sites to glucocorticoid hormones in peripheral blood leukocytes of normal subjects, patients with unstable angina pectoris and patients with myocardial infarction. a sharp (2-3-fold) increase in the number of binding sites was observed in patients with myocardial infarction during the first 24 hours after the onset of the disease. | 1986 | 3801148 |
| reduced number of [3h]nicotine and [3h]acetylcholine binding sites in the frontal cortex of alzheimer brains. | nicotinic cholinergic receptors were measured in human frontal cortex using [3h]nicotine and [3h]acetylcholine (in the presence of atropine) as receptor ligands. a parallel marked reduction in number of [3h]nicotine (52%; p less than 0.01) and [3h]acetylcholine (-55%; p less than 0.05) binding was found in the frontal cortex of alzheimer brains (ad/sdat) when compared to age-matched control brains. as a comparison the number of muscarinic receptors was quantified using [3h]quinuclidinyl benzilat ... | 1986 | 3808458 |
| [effect of exertion on the tropomyosin level in rat skeletal muscles]. | it is established that the content of tropomyosin in skeletal muscles of rats increases due to the action of the systematic physical exercises of the speed-force direction. suprareduction of tropomyosin in remote periods of rest after a single load is a biochemical basis of this process. | 1987 | 3810900 |
| nonproductive, cell-associated virus exists before the appearance of antiviral antibodies in experimental measles encephalitis. | the ability of measles virus to induce a cell-associated state of infection in the central nervous system (cns) plays an important role in the pathogenesis of subacute sclerosing panencephalitis (sspe), a fatal disease of children and young adults. the mechanism by which such an infection state is established is unknown, but several hypotheses have been proposed. one of these suggests that antiviral antibodies induce the nonproductive infection state by a process termed antigenic modulation. res ... | 1987 | 3811233 |
| [comparative study of the activity of arginase isoenzymes in brain tumors of humans and experimental animals]. | isoenzyme spectrum and total activity of arginase were studied in rat brain during growth of transplanted neurinoma and glioma as well as in malignant tissues of human brain. after transplantation of the tumors two peaks of arginase activation were observed within 2-4 days in rat brain tissues and within 16 days in tumoral tissue. positively charged isoenzyme i of arginase was mainly activated but activity of neutral isoenzyme ii was unaltered or slightly decreased. in human brain tumors activit ... | 1986 | 3811280 |
| [effect of bile on the development of tumors of the stomach and small intestine induced in rats by n-methyl-n-nitro-n-nitrosoguanidine]. | the study of the influence of long-term injection of whole bile into rat's stomach on n-methyl-n-nitro-n-nitrosoguanidine-induced tumors revealed an increased frequency of stomach malignancies matched by a lower frequency of those in the small intestine as well as slower rates of growth of gastrointestinal tumors. | 1987 | 3811314 |
| dynamic model of the equine hindlimb during the swing phase. | a dynamic model is developed to describe the swing phase of the hindlimb of a normally walking horse. the limb was represented by four rigid segments constrained to move in a sagittal plane only. the mathematical equations of motion of this four-element pendulum were formulated using lagrange's theorem. the morphometric parameters from the hindlimb segments of 3 horses were determined using high-speed film analysis. five muscle groups were incorporated in the model. muscle activity was derived f ... | 1986 | 3811820 |
| human term placenta releases placental protein 10 (pp10) in tissue culture. | placenta, decidua, chorion and amnion were studied to determine the tissue concentration of placental protein 10 (pp10). as measured by radio-immunoassay, all tissues studied were found to contain approximately the same concentration of pp10, in both early and late pregnancy. the release of pp10 was studied in tissue culture, first by using explants of placenta, decidua, amnion and chorion in four preliminary studies. only the placenta released significant amounts of pp10 into the culture medium ... | 1986 | 3811843 |
| antibacterial activity and ototoxicity in guinea pigs, and nephrotoxicity in rats of arbekacin. | arbekacin (hbk), 1-n[(s)-4-amino-2-hydroxybutyl]-3',4'-dideoxykanamycin b, showed broad antibacterial spectra against gram-positive and gram-negative bacteria including pseudomonas aeruginosa. it was also effective against gentamycin- or tobramycin-resistant bacteria. hbk was resistant to various aminoglycoside-inactivating enzymes except for aac (2') and aac (6')-iv, both of which slowly inactivated it. even at higher dosages (150 mg/kg i.m. or greater, which resulted in some deaths), hbk never ... | 1986 | 3814212 |
| gangliosides of human melanoma: altered expression in vivo and in vitro. | the ganglioside composition of human melanoma was analyzed in five sets of tumor specimens obtained directly from surgery, from the autologous tissue culture cell lines, and from the autologous cell lines grown in athymic nude mice. total gangliosides of these 15 melanoma specimens were isolated and purified, and the amount of each component ganglioside was analyzed by thin-layer chromatography and a thin-layer chromatography scanner. the ganglioside composition of the five surgical melanoma spe ... | 1987 | 3815339 |
| resistance to pesticin, storage of iron, and invasion of hela cells by yersiniae. | the independent abilities of yersinia pestis to absorb exogenous pigments including hemin and congo red (pgm+) and to produce the bacteriocin pesticin with genetically linked invasive enzymes (pst+) are established virulence factors of the species. pst- pgm+ strains of y. pestis are sensitive to pesticin (psts), and mutation of these isolates to pesticin resistance (pstr) is known to result in concomitant conversion to pgm-. wild-type cells of yersinia pseudotuberculosis and yersinia enterocolit ... | 1987 | 3818085 |
| plague in a free-ranging mule deer from wyoming. | | 1987 | 3820418 |
| effect of sulfadimethoxine-ormetoprim in the treatment of calves with induced pasteurella pneumonia. | the efficacy of sulfadimethoxine (sdm)-ormetoprim (omp) was evaluated in calves with induced pasteurella pneumonia. a dose-titration study comparing 3 doses of sdm-omp was performed to determine the optimal dose. treatments included: group 1--nontreated controls; group 2--33 mg of sdm-omp/kg of body weight, orally on day 1 and 17 mg/kg on days 2 to 5; group 3--66 mg of sdm-omp/kg, orally on day 1 and 33 mg/kg on days 2 to 5; group 4--99 mg of sdm-omp/kg, orally on day 1 and 50 mg/kg on days 2 to ... | 1987 | 3826837 |
| cardiovascular and neurological effects of laudanosine. studies in mice and rats, and in conscious and anaesthetized dogs. | the effects of laudanosine, a metabolite of atracurium, on the behaviour of conscious mice, rats and dogs, and on cardiovascular function in conscious and anaesthetized dogs have been evaluated: eeg studies were performed in anaesthetized dogs. in mice and rats, i.v. bolus doses of laudanosine 10-20 mg kg-1, caused convulsions and hind limb extensions; these effects were prevented by pretreatment with diazepam. after the continuous infusion of laudanosine to conscious dogs, plasma concentrations ... | 1987 | 3828170 |
| [specific protein in the plasma of rheumatoid arthritis patients]. | a unique protein was detected in the plasma of rheumatoid arthritis patients by two-dimensional electrophoresis. we named it rheumatoid arthritis specific protein (rasp). according to the pattern and concentration of its spot, ra patients were classified into three groups as rasp+, rasp +/- and rasp-. rasp+ was detected only in ra and its occurrence was 45/120 (38%). the occurrence of rasp including rasp +/- was 84/120 (70%) in ra, 6/13 (46%) in sle, 2/8 (25%) in pss, 11/51 (22%) in liver diseas ... | 1985 | 3831162 |
| double osteotomy operation in the surgical treatment of coxa plana disease. | during the years 1975 to 1982, what was named the double osteotomy--which is a combination of pelvic osteotomy together with subtrochanteric varus, extension and derotation osteotomy--was applied to 26 cases suffering from coxa plana (perthes-calvé-legg disease). the postoperative follow up averages a period of three years and five months and was undertaken at the department of orthopedics and traumatology of the faculty of medicine, university of ankara, turkey. | 1985 | 3832036 |
| [isolation of yersinia pestis in plague foci in northeast brazil from 1966 to 1982]. | | 1985 | 3832341 |
| [effect of titanium phosphate, zirconium oxide and a mixture of ammonium phosphotungstenate with zirconium phosphate on the elimination of radioactive cesium from the bodies of rats]. | | 1985 | 3835674 |
| [effect of polyphenols of coffee pulp on iron absorption]. | the effect of the polyphenols of coffee pulp on iron absorption was studied using the method of ligated segments in rats. optimal conditions to measure iron absorption, were determined using as criteria the concentration of fe59 and the time that produced the highest value of blood radioactivity. a concentration of 0.4 uci/dose of fe59 and a 3-hr period were chosen to measure iron absorption. experimental groups were formed assigning six rats randomly to each group. each group was injected with ... | 1985 | 3836605 |
| involvement of iron and copper in biological damage that is induced by oxygen free radicals. | | 1985 | 3837800 |
| plasma cell leukemia accompanied by polyclonal gammopathy and congenital tumor in the atrioventricular node. | a 32-year-old man with plasma cell leukemia and congenital tumor of the atrioventricular node is described. although m protein could not be detected by immunoelectrophoresis, the examination of bone marrow atypical plasma cells with immunofluorescent and immunoperoxidase showed that most of these plasma cells produced igg or iga. also some plasma cells possessed concomitantly both igg and iga. an electrocardiogram revealed complete atrioventricular block. the patient died of aspergillosis and bl ... | 1985 | 3837948 |
| [heterogeneity of monoclonal antibody affinity]. | in experiments with pasteurella pestis monovalent capsular antigen and hybridoma monoclonal antibodies obtained after cloning and recloning the heterogeneity of the active centers of antibodies with respect to their affinity was revealed. in ascitic fluids obtained from the animals inoculated with different hybridoma clones 5 groups of antibodies, differing in their affinity, were determined in each fluid sample. | 1985 | 3838847 |
| heterogeneity of membrane phospholipid mobility in endothelial cells depends on cell substrate. | cellular growth control and differentiation have been shown to be dependent on both cell-cell and cell-substrate contacts. interactions of cells with extracellular material are critical events during embryonic development and maintenance of tissue function. plasma membrane receptors have been described for components of the extracellular matrix such as fibronectin, laminin and various collagen types. transmembrane signalling has been shown to be influenced by the lateral mobilities of the plasma ... | 1985 | 3839906 |
| the action of the yersinia pestis phage on the plague envelope antigen and the human erythrocytes. | the activity of the yersinia pestis phage on the plague envelope antigen has been described. the purified envelope antigen was shown to possess the receptor properties. the phage caused changes of viscosity and hemagglutination properties of the antigen. the phage-treated antigen showed the additional precipitation arc in the immunoelectrophoretic investigations. the adsorption of the yersinia pestis phage on the sheep and human erythrocytes was examined. the human red cells of group b exhibited ... | 1985 | 3841818 |
| the effect of toosendanin on monkey botulism. | | 1985 | 3849628 |
| theoretical mechanisms for synthesis of carcinogen-induced embryonic proteins: xiv. mutational and non-mutational mechanisms as subsets of a more general mechanism. part c. a defined cancer mutation. | the results of applying a mechanism of ethionine-induced embryonic gene derepressions to explain similar features found in hereditary tyrosinemia have been extended to a well defined cancer mutation. in all three cases, the described mechanism is compatible with the explanation for the etiology of embryonic like phenotypic expressions in cells and potentially for the carcinogenic process. the essence of the formulated process for a human bladder carcinoma mutation in the ras gene for a protein p ... | 1985 | 3853079 |
| [the plague]. | | 1985 | 3854706 |
| quantitative assessment of the role of o6-methylguanine in the initiation of carcinogenesis by methylating agents. | induction of transformation, cell lethality, and dna lesions were quantitatively compared in syrian hamster embryo cells (hec) treated with three different methylating agents: n-methyl-n'-nitro-n-nitrosoguanidine (mnng), n-methyl-n-nitrosourea (mnu), or methyl methanesulfonate (mms). each induced transformation in a dose-dependent manner. on a molar basis, mnng was approximately equal to 100- and 500-fold more effective than mnu and mms, respectively. for each carcinogen the induction and repair ... | 1985 | 3855560 |
| recognition of a leukemia-related antigen by an antiidiotypic antiserum to an anti-gp70 monoclonal antibody. | the possibility that receptors for retroviral gp70 share structural elements with the antigen-binding sites of anti-retroviral gp70 antibodies was investigated. a monoclonal antibody (1416) was produced that reacted with the gp70 of a cloned recombinant leukemogenic retrovirus, termed p1. an antiidiotypic antiserum raised to 1416 was tested for its ability to bind to the thymic leukemia induced by p1 (p1 thy). a membrane structure was identified on the surface of p1 thy that reacted with the ant ... | 1985 | 3856626 |
| human monocyte-endothelial cell interaction in vitro. | we have examined the interaction of freshly isolated human blood monocytes with cultured human umbilical vein endothelial cells in vitro. purified monocytes incubated with confluent primary or passaged endothelial cells (ec) for 90 min at 37 degrees c bound at maximal densities of 6.5-7.0 x 10(3)/mm2 (8 or 9 per ec) without causing disruption of the monolayer. monocyte-ec binding proceeded in the presence of plasma proteins or optimal phagocytic doses of opsonized zymosan particles. the avidity ... | 1985 | 3865221 |
| [community-acquired pneumonias: importance of pneumococcal pathology]. | the frequency and the various etiologies of community acquired pneumonia are not well established. in adults, it is believed that half of the cases are caused by viruses and mycoplasma pneumoniae. the pneumonia in these patients is limited and treated on an ambulatory basis. in patients who need to be hospitalized, the main etiology of pneumonia is s. pneumoniae. despite effective antibiotic treatment, pneumococcal pneumonia still remains a life-threatening infection, especially in bacteremic pa ... | 1985 | 3871965 |
| reactivities of neoplastic t cells to human autoantibody and monoclonal antibodies. | | 1985 | 3873778 |
| release of interleukin i and low-molecular-weight lymphocyte-activating factors by rat peritoneal macrophages and its enhancement by acute non-specific inflammatory processes. | peritoneal macrophages harvested from rats undergoing an acute non-specific inflammatory reaction induced by an injection of calcium pyrophosphate (capp) into the pleural cavity released increased amounts of interleukin i (il-i)-like material. lymphocyte-activating factors were also found in ultrafiltrates of the macrophage supernatants below 10 kd and 5 kd. a similar pattern of activity was observed when lysates of the macrophages were tested. in addition pre-exposure of normal peritoneal macro ... | 1985 | 3873954 |
| aids: discrimination issues. | | 1985 | 3880472 |
| aids: discrimination issues. | | 1985 | 3880472 |
| integration of the vwa plasmid into the chromosome of yersinia pestis strains harboring f' plasmids of escherichia coli. | conditional virulent strains of yersinia pestis were used as recipients of an f-lac plasmid from either escherichia coli 23.10s or e. coli csh23. the transconjugants of y. pestis were calcium independent; however, calcium dependence was restored after the loss of the e. coli plasmid. the plasmid contents of several y. pestis f-lac clones were compared with those of the parent strains and several lac segregants. although the 6- and 61-megadalton plasmids were detected in all strains tested, the v ... | 1985 | 3882563 |
| transfer of the virulence plasmid of yersinia pestis to yersinia pseudotuberculosis. | transposon tn5 insertion derivatives of the virulence plasmid pyv019 of yersinia pestis were transferred by p1 transduction into a plasmid-free strain of y. pseudotuberculosis. one of these plasmid derivatives conferred virulence upon the y. pseudotuberculosis strain. this strain had the ability to express temperature-inducible plasmid-coded outer membrane proteins and was also found to be ca2+ dependent. | 1985 | 3884511 |
| cigarette smoking--induced enhancement of platelet function: lack of prevention by aspirin in men with coronary artery disease. | increased cardiovascular morbidity and mortality among cigarette smokers may be mediated in part by enhanced platelet function. previous data showed that cigarette smoking--induced lowering of the platelet aggregate ratio of normal individuals was prevented by taking aspirin before smoking. our study was undertaken to determine whether similar results would occur in men with coronary artery disease and whether platelet factor 4 would be released. a random-order, double-blind crossover study comp ... | 1985 | 3884719 |
| synthesis by mouse peritoneal cells of g-csf, the differentiation inducer for myeloid leukemia cells: stimulation by endotoxin, m-csf and multi-csf. | normal c57bl mouse peritoneal cells were able to synthesize material with the ability to induce differentiation in colonies of the mouse myelomonocytic leukemia cell line, wehi-3b. the active factor was provisionally identified biochemically as the normal regulator, granulocyte colony-stimulating factor, g-csf. thioglycollate-induced peritoneal exudate cells had little or no capacity to synthesize such material. production of active material was elevated 10-100-fold by exposure of peritoneal cel ... | 1985 | 3887043 |
| [fleas immune to plague studied by a serological method]. | | 1985 | 3887115 |
| [effectiveness of various methods of exterminating fleas in natural foci of plague (review of the literature)]. | | 1985 | 3887116 |
| [experimental studies on the behavior of intracranial pressure under prostacyclin application]. | in recent experimental studies the beneficial therapeutic effect of the arachidonic acid metabolite prostacyclin (pg 12) has been demonstrated for the treatment of different states of impaired brain perfusion following subarachnoid hemorrhage or focal cerebral ischemia. prostacyclin has been recommended especially for the possible prevention and treatment of cerebral vasospasm after aneurysm rupture. because prostacyclin is a potent vasodilator of the cerebral circulation it is an essential prer ... | 1985 | 3887195 |
| [experimental studies on the behavior of intracranial pressure under prostacyclin application]. | in recent experimental studies the beneficial therapeutic effect of the arachidonic acid metabolite prostacyclin (pg 12) has been demonstrated for the treatment of different states of impaired brain perfusion following subarachnoid hemorrhage or focal cerebral ischemia. prostacyclin has been recommended especially for the possible prevention and treatment of cerebral vasospasm after aneurysm rupture. because prostacyclin is a potent vasodilator of the cerebral circulation it is an essential prer ... | 1985 | 3887195 |
| ethanol-induced genotoxicity. | unrelated, nondenatured, ethanol preparations, derived synthetically or by fermentation, were found to induce qualitatively similar concentration-dependent toxic and genotoxic effects as measured by rk mutatest. in this system ethanol was found genotoxic above a threshold concentration of 18-19% (v/v) when rk+ selector cells were transiently exposed for 10 min before selection for rk- survivors at 42 degrees c. | 1985 | 3889601 |
| repair of dna single-strand breaks in x-irradiated yeast. ii. kinetics of repair as measured by the dna-unwinding method. | the kinetics of disappearance of single-strand breaks (ssb) from the dna of x-irradiated stationary yeast cells under liquid-holding conditions was found to proceed in a dose-independent manner up to a dose of at least 2400 gy, and was found to be complete after incubation of cells for 1 h. this was deduced from data for a yeast wild-type (wt) haploid and diploid strain as well as for rad52 haploid cells defective in dna double-strand break (dsb) repair. in all cases an initial fast repair compo ... | 1985 | 3889609 |
| tiapamil--a new calcium antagonist. | calcium antagonists are an important new modality in cardiovascular therapy. tiapamil, a congener of verapamil, is undergoing clinical and laboratory evaluation. we have undertaken experimental studies on the anti-arrhythmic properties of tiapamil, and on the intravenous use of this agent in patients with acute myocardial infarction. reasons are given for suggesting that tiapamil warrants further clinical evaluation, after which it may join the more established calcium antagonists as a valuable ... | 1985 | 3890216 |
| a new serine protease which preferentially recognizes p-guanidino-l-phenylalanyl residue in ascitic plasma from ehrlich ascites tumor-bearing mice. | a new enzyme which hydrolyzes anilide substrates of p-guanidino-l-phenylalanine in preference to those of arginine was found in the ascitic plasma from ehrlich ascites tumor-bearing mice. the activity of this enzyme on n alpha-benzyloxycarbonyl-p-guanidino-l-phenylalanine p-nitroanilide was strongly inhibited by diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride but not by sulfhydryl-reactive reagents and metal chelating agents. peptide substrates containing p-guanidino-l-phenylalani ... | 1985 | 3890849 |
| a new serine protease which preferentially recognizes p-guanidino-l-phenylalanyl residue in ascitic plasma from ehrlich ascites tumor-bearing mice. | a new enzyme which hydrolyzes anilide substrates of p-guanidino-l-phenylalanine in preference to those of arginine was found in the ascitic plasma from ehrlich ascites tumor-bearing mice. the activity of this enzyme on n alpha-benzyloxycarbonyl-p-guanidino-l-phenylalanine p-nitroanilide was strongly inhibited by diisopropyl fluorophosphate and phenylmethanesulfonyl fluoride but not by sulfhydryl-reactive reagents and metal chelating agents. peptide substrates containing p-guanidino-l-phenylalani ... | 1985 | 3890849 |
| a possible role for natural killer cells in providing protection against plasmodium berghei in early stages of infection. | beige mutant mice, which are deficient in natural killer (nk) cells, exhibited a significantly higher parasitaemia than the parental c57bl/6 strain between days 4 and 10 after infection with plasmodium berghei. within 8-12 days after infection 70% of beige mice were dead but no deaths occurred in the parental strain until day 16. the median survival time of the beige mice (10 days) was significantly lower than that of the parental strain (22 days). it appears that nk cells may be protective in t ... | 1985 | 3891603 |
| modulation of polymorphonucleate-mediated cytotoxicity against candida albicans by thymosin alpha 1. | in the present work we analyze the effects of thymosin alpha 1 treatment on the number and the candidacidal activity of murine polymorphonuclear leukocytes. the data we obtained showed that the treatment with thymosin alpha 1 (100 micrograms/kg s.c.) 10, 8, 6, 4 and 2 days before the assay may result in a significant numerical augmentation of circulating polymorphonucleates in the peripheral blood, as well as of their candidacidal activity when measured in vitro in both a 4-h cytotoxicity assay ... | 1985 | 3892779 |
| an improved indirect enzyme-linked immunosorbent assay (elisa) for the detection of antisperm antibodies. | an indirect enzyme-linked immunosorbent assay (elisa) was used for the detection of antisperm antibodies which provides a sensitivity and ease of quantitation that is not available with conventional bioassay systems. a standardized protocol was developed in which washed whole sperm were coated onto polystyrene microelisa plates at a density of 1 x 10(5) sperm per well using a commercially available spray fixative. urease was employed in the enzyme-antiimmunoglobulin conjugate to minimize nonspec ... | 1985 | 3893172 |
| protection against yersinia infection induced by non-virulence-plasmid-encoded antigens. | specific immunity against yersinia was induced by plasmid-encoded antigens not associated with virulence. mice were immunised with viable bacteria from a virulence-plasmid-cured strain of y. pseudotuberculosis. this antigenic stimulation generated specific protection against virulence-plasmid-harbouring strains of y. pseudotuberculosis and y. pestis, demonstrating that protection can be generated by organisms lacking plasmid-encoded virulence antigens. | 1985 | 3900406 |
| role of a plasmid in the pathogenicity of yersinia species. | | 1985 | 3902382 |
| properties of an escherichia coli mutant deficient in phosphoenolpyruvate carboxylase catalytic activity. | a mutant escherichia coli (ppcc-) which was unable to grow on glucose as a sole carbon source was isolated. this mutant had very low levels of phosphoenolpyruvate carboxylase activity (approximately 5% of the wild type). goat immunoglobulin g prepared against wild-type phosphoenolypyruvate carboxylase cross-reacted with the ppcc- enzyme. the amount of enzyme protein in the mutant cells was similar to that found in wild-type cells, but it had greatly diminished specific activity. the catalyticall ... | 1985 | 3902793 |
| splenectomy in experimental pancreas transplantation. | | 1985 | 3904093 |
| [taxonomy of the causative agents of plague and pseudotuberculosis]. | | 1985 | 3904284 |
| [use of monoclonal antibodies for the production of a plague antibody erythrocyte diagnostic agent]. | monoclonal antibodies to yersinia pestis capsular antigen were fixed onto the surface of formulated sheep red blood cells. the preparation thus obtained was compared with commercial antibody erythrocyte diagnosticum in the passive hemagglutination test aimed at the search for the capsular antigen in the suspensions of yersinia pestis museum cultures and in the antigen neutralization test aimed at the search for antibodies in the sera of wild and laboratory animals having had plague. monoclonal e ... | 1985 | 3904286 |
| [use of beta-lactamase from bacillus licheniformis 749/c for solid-phase immunoenzyme analysis]. | beta-lactamase from bacillus licheniformis 749/c was used as a marker for elisa. conjugates of the beta-lactamase with the capsule antigen of the plague causative agent and with the monoclonal antibodies to it were prepared by glutaric aldehyde "linking". the conjugates preserved high immunospecific and beta-lactamase activity. high sensitivity of the modification of elisa allowed detecting up to 8 ng/ml of the capsule antigen of the plague causative agent. the enzymatic activity of the beta-lac ... | 1985 | 3904622 |
| morphological and functional changes in several endocrine glands induced by hypothyroidism in the rat. | the effect of hypothyroidism upon the morphology and the function of several endocrine glands was studied in radiothyroidectomized male rats. it was found that t3, t4, insulin, prolactin and corticosterone levels were significantly lower in hypothyroid rats. tsh levels were significantly higher in these animals while no changes were depicted in testosterone levels. the administration of t4 drew back to normal range the above-mentioned altered serum hormone levels. the studies performed with ligh ... | 1985 | 3907250 |
| establishment of pregnancy and its immunological implications in the pig. | the consideration of the fetus as a semi-allograft by virtue of its paternally inherited histocompatibility antigens is generally accepted. many hypotheses have been put forward to explain the immunological acceptance of the conceptus by the maternal immune system, but no single theory has proved to be adequate. most studies so far have been carried out with laboratory rodents and men but corresponding investigations of farm animals and especially the pig are limited. because striking difference ... | 1985 | 3910828 |
| calcitonin cell population and distribution in the thyroid gland of the rat. | calcitonin-containing cells (c cells) were identified in male wistar white rats using an immunoperoxidase technique. they occupied a central position within the thyroid; very few were found peripherally, inferiorly, and superiorly; and none were present in the isthmus. the number of calcitonin-containing cells present per gram of body weight increased with age up to 70 days and had declined by 100 days. determining the true total c-cell count through the entire thyroid is a very laborious proced ... | 1985 | 3910845 |
| relation between antibiotic resistance and number of plasmids in enterotoxigenic and non-enterotoxigenic escherichia coli strains. | we have investigated the relation between antibiotic resistance and the number of plasmids contained in 44 enterotoxigenic (etec) and 69 non-enterotoxigenic (non-etec) escherichia coli strains. both etec and non-etec strains were resistant to one or more antibiotics at a similar rate, but multiple resistance in resistant non-etec strains was higher than in resistant etec strains, showing a significant difference (p less than 0.001) when subjected to student's t test. among the etec strains, thos ... | 1985 | 3911036 |
| [evaluation of the sensitivity of haemophilus to antibiotics by the modified api-atb system]. | a simple micromethod in a liquid medium using the api-atb system was developed for testing the susceptibility of haemophilus to antibiotics. to evaluate this method, 50 strains, including 12 beta-lactamase producers, were studied. results were compared to those obtained using mic determination in a liquid medium (reference) and an agar diffusion method (routine). for all three techniques, a mueller-hinton medium enriched in hemoglobin and nad was used, and cultures were incubated at 37 degrees c ... | 1985 | 3911154 |
| [comparative study of lipoteichoic acid from streptococcus pyogenes type 29 isolated by different methods]. | a comparative study of lipoteichoic acid preparations extracted from streptococcus pyogenes with cold and hot phenol and trichloracetic acid was made. the most delicate way for isolation of lipoteichoic acid from the given microorganisms is cold phenol extraction. | 1985 | 3911202 |
| photochemical modifications of lac repressor: effect of effectors binding on tryptophan photooxidation. | | 1985 | 3911222 |
| [changes in the oxygen-dependent metabolic activity of polymorphonuclear leukocytes from the peripheral blood and macrophages from the peritoneal exudate in the experimental infection of mice with the causative agent of plague]. | the subcutaneous infection of c57bl/6j mice and noninbred white mice with 40 ld100 of y. pestis virulent strain has been found to produce synchronous changes in the oxygen-dependent metabolism (odm) of peripheral blood neutrophils in the spontaneous or zymosan-, e. coli- and y. pestis-stimulated variants of the nbt test. these changes can be divided into three phases: (i) the phase of a sharp drop in odm activity; (ii) the phase of the increase of this activity, occurring simultaneously with the ... | 1985 | 3911675 |
| [preparation and use of magnetic sorbents for studying microorganism antigens]. | in this work simple techniques for obtaining polyacrylamide sorbents with magnetic properties are described. these techniques have permitted obtaining block and microgranulated sorbents with the immobilization of antibodies from plague antiserum in the cellular gel structure for the specific sorption of killed and live yersinia pestis cells and their first fraction; pig brain gangliosides have also been incorporated into the gel structure with a view to the sorption of cholera toxin from the fil ... | 1985 | 3911684 |
| [serine trypsin-like proteinase from the cells of the causative agent of plague yersinia pestis]. | | 1985 | 3912146 |
| [purification of the dna-dependent rna-polymerase of yersinia pestis and its characteristic properties]. | | 1985 | 3916962 |
| biosynthesis of cholestanol from bile acid intermediates in the rabbit and the rat. | biliary 7 alpha-hydroxy-4-cholesten-3-one (an intermediate in bile acid biosynthesis) may be 7 alpha-dehydroxylated in the gut and further metabolized to cholestanol (skrede, s., and björkhem, i. (1982) j. biol. chem. 257, 8363-8367). we have now evaluated the quantitative importance of pathway(s) to cholestanol with 7 alpha-hydroxylated c27 steroids as intermediates. after feeding conventionally fed rabbits or rats or germ-free rats with [7 alpha-3h]cholesterol and [4-14c]cholesterol, tissue ch ... | 1985 | 3917436 |
| modification of bronchial hyperreactivity after treatment with sodium cromoglycate during pollen season. | repeated bronchial histamine challenges before, during, and after the birch pollen season were performed in 22 allergic patients with bronchial hyperreactivity (bhr) treated for 6 wk with sodium cromoglycate (scg), 20 mg, four times a day, or placebo in a double-blind, randomized group comparison. clinical assessments of the asthmatic symptom score and peak expiratory flow revealed less symptoms and less use of bronchodilators in the scg group. responsiveness to histamine was significantly incre ... | 1985 | 3920301 |
| intracellular gas supersaturation tolerances of erythrocytes and resealed ghosts. | intact mammalian, avian, and amphibian erythrocytes were saturated with up to 300 atm nitrogen or argon gas and rapidly decompressed. despite the profuse nucleation of gas bubbles in the suspending fluid, no evidence of intracellular gas bubble nucleation was found; all or most of the cells remained intact and little or no hemoglobin escaped. internal bubbles were similarly absent from resealed ghosts of human erythrocytes as shown by lack of disintegration and by retention of an entrapped fluor ... | 1985 | 3921076 |
| regulation of cardiac vulnerability by the cerebral defense system. | psychosocial stressors are risk factors for sudden cardiac death. a theoretical model of the brain mechanism that links defined environmental events (stressors) to cardiac vulnerability (initiation of ventricular fibrillation) has been developed. in the model, a stressor event evokes a set of electrochemical responses in the frontal cortex. depending on the state of acquisition of these electrochemical responses to the stressor, activity will or will not be initiated in the frontocortical-brains ... | 1985 | 3923079 |
| effect of mannitol on cerebral blood flow and cerebral perfusion pressure in human head injury. | patients with severe head injury frequently have evidence of elevated intracranial pressure (icp) and ischemic neuronal damage at autopsy. mannitol has been used clinically to reduce icp with varying success, and it is possible that it is more effective in some types of head injury than in others. the aim of the present study was to determine the effect of mannitol on icp, cerebral perfusion pressure (cpp), and cerebral blood flow (cbf) in patients with severe head injury, and to discover if the ... | 1985 | 3925092 |
| [characteristics of the capsular antigen preparations isolated from a broth culture of yersinia pestis strain ev]. | the physico-chemical and immunobiological study of the preparation of capsular antigen, isolated by a high-yield method from the culture fluid of y. pestis grown in nutrient broth at 37 degrees c and practically free from the admixture of polysaccharide and nucleic acids, has been made. the study has revealed that this capsular antigen is protein with a molecular weight of 1.65-1.69 x 10(6) d and sedimentation constant equal to 13.4s; its viscosity is 220 ml/g and its isoelectric point is at ph ... | 1985 | 3925663 |
| pressure- and temperature-dependent adhesion of pseudomonas aeruginosa to hep-2 cells. | pseudomonas aeruginosa bacteria adhere to human epitheloid (hep-2) cells in culture. under normal atmospheric conditions (1 ata), this adhesion increased significantly when the temperature rose from 22 to 37 degrees c. under hyperbaric atmosphere (= air, 7 ata) conditions, a similar, significant enhancement of bacterial adhesion to the cells was noted when the temperature rose. if the temperature was kept stable at 22 or 37 degrees c and the pressure was increased from 1 to 7 ata, a pressure-ind ... | 1985 | 3925713 |
| [pharmacological study of benzylpenicillin-benzamido-methyl ester. i. in vivo chemotherapeutic properties]. | | 1985 | 3925715 |
| the effects of aerosols of carbamylcholine, serotonin and propranolol on the ventilatory response to co2 in guinea pigs and comparison with the effects of histamine and sulfuric acid. | a new method is introduced to recognize the acute pulmonary effect of airborne contaminants in guinea pigs. the method avoids anaesthesia and invasive techniques and requires minimal restraint of the animal. to recognize the acute pulmonary effect, the inspiratory volume of the animal was increased by continuous inhalation of a mixture containing 10% co2 in 18% o2 and 72% n2. once a plateau increase in inspiratory volume was reached, the aerosol to be studied was added to this mixture and its ef ... | 1985 | 3925716 |
| limitations, risks and complications of laser recanalization: a cautious approach warranted. | | 1985 | 3925749 |
| effect of naloxone on luteinizing hormone, follicle-stimulating hormone, and prolactin secretion in the different phases of the estrous cycle. | it is becoming increasingly clear that the effects exerted by opioid agonists and antagonists on the release of gonadotropins and of prl may vary according to the endocrine milieu. to investigate this issue further, female rats with a regular 4-day estrous cycle have been injected sc with the opioid antagonist naloxone at different hours of the day, during each of the various days of the estrous cycle. the animals were killed 20 min after the sc administration of naloxone (2.5 mg/kg dissolved in ... | 1985 | 3926461 |
| experimental intestinal obstruction in cattle. part i: changes in the clinical picture. | | 1985 | 3927617 |
| spectroscopic studies of pyruvate oxidase flavoprotein from escherichia coli trapped in the lipid-activated form by cross-linking. | pyruvate oxidase is a flavoprotein dehydrogenase isolated from escherichia coli which catalyzes the oxidative decarboxylation of pyruvate to acetate plus co2. the maximal turnover of the enzyme, measured using a ferricyanide reductase assay, is increased 20-to 30-fold by either of two methods. proteolysis in the presence of the substrate (pyruvate) and cofactor (mg2+-thiamin pyrophosphate) results in cleavage at a single locus near the carboxyl terminus and concomitant activation. phospholipids ... | 1985 | 3928620 |
| identification of proteolytic pseudomonads isolated from raw milk. | proteolytic pseudomonads isolated from raw milk were classified by numerical taxonomy. unweighted pair-group average-linkage cluster analysis was used to cluster 49 bacterial strains, of which 26 were pseudomonas species, as described in the bergey's manual of determinative bacteriology, based on 52 characters. the milk isolates resided in two clusters; one containing pseudomonas fluorescens and the other pseudomonas fragi. the isolates identified with pseudomonas fluorescens hydrolyzed milk pro ... | 1985 | 3930583 |
| synthesis of dihomoprostaglandins from adrenic acid (7,10,13,16-docosatetraenoic acid) by human endothelial cells. | human umbilical vein endothelial cells were found to contain adrenic acid (22:4) in their cellular lipids. since this fatty acid may be metabolized by cyclooxygenase in the kidney, the metabolism of adrenic acid was studied in endothelial cell cultures. [14c]adrenic acid was metabolized to several more polar metabolites. two of these metabolites co-migrated on hplc with 1 alpha,1 beta-dihomo-8-ketoprostaglandin f1 alpha (the metabolite of 1 alpha, 1 beta-dihomoprostaglandin i2) and 1 alpha,1 bet ... | 1985 | 3931686 |
| penetration of aflatoxins through isolated human epidermis. | the purpose of this study was to determine if aflatoxin b1 (afb1) could penetrate through isolated human epidermis (stratum corneum plus viable epidermis). [14c]afb1 (7.5-9.3 micrograms) was applied to the stratum corneum of epidermal disks mounted in teflon diffusion cells. [14c]afb1 penetrated chemically unaltered through the isolated epidermis. chloroform-extractable radioactivity accounted for 82.5 +/- 3.7% of the total penetrating radioactivity in the receptor fluid of the diffusion cells. ... | 1985 | 3932668 |
| goat's milk: legal, disease risk and microbiological considerations. | | 1985 | 3934373 |
| goat's milk: legal, disease risk and microbiological considerations. | | 1985 | 3934373 |
| plague in the united states, 1984. | | 1985 | 3934524 |
| follicle regulatory protein noncompetitively inhibits microsomal 3 beta-hydroxysteroid dehydrogenase activity. | a heat- and trypsin-labile follicular fluid protein (frp) extracted from both human and porcine follicular fluid has been shown to modulate ovarian steroidogenesis. to further investigate the effects of frp, its effect on the kinetics of 3 beta-hydroxysteroid dehydrogenase activity (3 beta-hsd) was evaluated in cell-free microsomal preparations from human placenta. test fractions of follicular fluid protein were preincubated with placental microsomes followed by the addition of various substrate ... | 1985 | 3935862 |
| inhibition of the differentiation of candida albicans by the chelator 1,10-phenanthroline. | several chelators were examined for their ability to prevent the synchronous release of 24- to 48-hour stationary phase singlet cells of the dimorphic yeast candida albicans into either the mycelial or the budding phenotypes (in a defined liquid medium at 37 degrees c; at ph 6.5 or ph 4.5, respectively). the only chelator that was found to inhibit mycelium formation completely and to restrict bud formation to about 10% was 1,10-phenanthroline at minimal concentrations of 50 microm and 230 microm ... | 1985 | 3937057 |
| comparative efficiency of primary light conversion in photosynthetic bacteria rhodospirillum rubrum and rhodopseudomonas viridis. | | 1985 | 3939722 |
| cross-reactivity of a monoclonal antibody directed against an estrogen-induced protein in mcf-7 human breast cancer cells with murine leydig cell tumor proteins. | an estrogen-responsive murine leydig cell tumor (m5480a) was examined for the presence of cross-reactive proteins to a monoclonal antibody directed against a mr 24,000 estrogen-regulated protein in human breast cancer cells. human breast tumor biopsies were used as controls for the cytosol preparations, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, and western blot conditions used in these experiments. the estrogen-regulated mr 24,000 protein was detected in sodium dodecyl sulfate-p ... | 1986 | 3940199 |
| the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats. | thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ... | 1986 | 3942673 |
| the addition of nitrous oxide to halothane decreases renal and splanchnic flow and increases cerebral blood flow in rats. | thirteen male sprague-dawley rats were anaesthetized with halothane and catheters were placed in the femoral artery and left ventricle. the animals breathed spontaneously through a tracheostomy throughout the investigation. after the surgical preparation, the inspired halothane concentration was maintained at 1.5 vol% (f/o2 = 0.3). after a 30-min stabilization period, strontium-85 radiolabelled microspheres (15 +/- 1 micron) were injected to the left ventricle for determination of cardiac output ... | 1986 | 3942673 |
| fate of n-(2-hydroxypropyl)methacrylamide copolymers with pendent galactosamine residues after intravenous administration to rats. | n-(2-hydroxypropyl)methacrylamide (hpma) copolymers bearing galactosamine residues accumulate in the liver after intravenous administration to rats (duncan, r., kopecek, j., rejmanová, p. and lloyd, j.b. (1983) biochim. biophys. acta 755, 518-521). in this study hpma copolymers bearing pendent galactosamine residues (1.0-11.6 mol%) were injected intravenously into rats and their rates of blood clearance and liver accumulation were measured. a level of substitution of 4 mol% was found to be suffi ... | 1986 | 3942780 |
| temperature-controlled plasmid regulon associated with low calcium response in yersinia pestis. | both the low calcium response and virulence in yersinia pestis strain kim5 are mediated by genes located on the 75.4-kilobase plasmid pcd1. the results presented here demonstrate the existence of two new genetic loci of pcd1 whose expression is regulated in response to temperature. levels of transcription in the trta and trtb loci were elevated 12- to 16-fold at 37 degrees c compared with levels of transcription at 30 degrees c. in addition, the absolute levels of transcription were the highest ... | 1986 | 3944056 |
| septicemic plague in a 14-month-old child. | | 1986 | 3945561 |
| plasmid-determined cytotoxicity in yersinia pestis and yersinia pseudotuberculosis. | yersinia pestis kim5 was found to be cytotoxic for the ic21 and p388d1 mouse macrophage cell lines, as well as for resident peritoneal macrophages from c57bl/6 mice. affected cells phagocytosed kim5 inefficiently, became spherical, detached readily from culture dishes, and retained 51cr poorly. the cytotoxic effect was dependent on the presence of the 75-kilobase plasmid pcd1. because this plasmid also encodes the low calcium response (lcr), three mu d1 insertion mutants previously shown to be l ... | 1986 | 3949380 |
| binding of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole to histamine receptors in guinea pig cerebral cortical membranes. | the interaction of 4(5)-[2-(4-azido-2-nitroanilino)ethyl]imidazole (aah), a photolabile histamine receptor antagonist, with the binding of histamine, mepyramine, and tiotidine to guinea pig cerebral cortical membranes was examined to evaluate the specificity of aah for histamine h1 and h2 receptors. saturable, specific binding of [3h]histamine, [3h]mepyramine, and [3h]tiotidine to the membranes was observed. competition assays were used to assess the relative affinity of aah for h1- and h2-recep ... | 1986 | 3952080 |