| evaluation with an iuc::tn10 mutant of the role of aerobactin production in the virulence of shigella flexneri. | to evaluate the role of aerobactin production in the virulence of shigella flexneri, a iuc::tn10 insertion mutant was obtained from strain m90t, a serotype 5 isolate. this mutant was tested for its ability to invade and kill hela cells in monolayers, to elicit keratoconjunctivitis in guinea pigs and to infect ligated segments of rabbit ileal loops. although this mutant did not grow in iron-depleted media, its ability to grow intracellulalry and eventually kill hela cells was unchanged from that ... | 1987 | 3040587 |
| the epidemiology of s. sonnei and s. flexneri in pima county, arizona: an exploratory study. | investigation of 189 cases of shigellosis reported to the pima county, arizona health department in 1986 revealed that 23 per cent of cases could be attributed to travel to mexico, and 10 per cent to day care attendees and their household contacts. no source of infection or high-risk activity could be demonstrated for 43 per cent of the cases. households in which s. flexneri occurred were more likely to be characterized by crowded living situations and to have no known source of infection. | 1988 | 3044149 |
| isolation of shiga toxin-resistant vero cells and their use for easy identification of the toxin. | shiga toxin-resistant vero cells were isolated by treatment of the cells with nitrosoguanidine. these mutant cells were not affected by shiga toxin at more than 1 microgram/ml, although the parent vero cells were sensitive to 25 pg of the toxin per ml. immunofluorescence studies showed that all the mutant cells had lost toxin-binding capacity. the cytotoxic activities of various bacterial cultures against the parent and mutant cells were compared. all samples from 10 strains of shigella dysenter ... | 1988 | 3045003 |
| congo red binding and salt aggregation as indicators of virulence in shigella species. | smooth strains of shigella dysenteriae type 1, shigella flexneri, shigella boydii, and shigella sonnei which form pigmented colonies (pcr+) on congo red agar were virulent in the sereny test. smooth variants unable to bind congo red (pcr-) were avirulent. measurements of dye uptake from solution showed that s. dysenteriae type 1 bound the most dye, followed in order of uptake by s. flexneri, s. boydii, and s. sonnei. using the salt aggregation test (sat) to determine cell surface hydrophobicity, ... | 1988 | 3045151 |
| clinical and microbiologic features of shigella and enteroinvasive escherichia coli infections detected by dna hybridization. | to determine the clinical and microbiologic features of shigella and enteroinvasive escherichia coli (eiec) infections, we investigated 410 children with diarrhea and 410 control children without diarrhea who were seen at children's hospital, bangkok, thailand, from january to june 1985. shigella spp. were isolated from 96 (23%) and eiec were isolated from 17 (4%) of 410 children with diarrhea and from 12 (3%) and 6 (1%) of 410 control children, respectively. the isolation rates of both pathogen ... | 1988 | 3045152 |
| glycocalyx in virulent and avirulent strains of shigella flexneri. | | 1988 | 3045162 |
| [morphologic manifestations of the medicinal lysis of a coronary artery thrombus]. | morphologic studies of coronary arterial thrombi have shown immature fibrin fibres to prevail in those, with fibrin degradation products always present. this fact suggests that fibrinolytic components of hemostasis, limiting the rates of fibrin polymerization and maturation, may participate in thrombogenesis. at the same time, macrophages that phagocytose fibrin and blood cells (platelets and erythrocytes) become activated since the early hours of the thrombus' existence. selective administratio ... | 1988 | 3045402 |
| [mechanism of the nonspecific protective action of shigella antigens]. | the mechanism promoting the nonspecific action of antigens obtained from s. flexneri and s. sonnei by a sparing method has been studied. these antigens stimulate the t- and b- systems of immunity, that is followed by activation of myelopoiesis and the humoral protective factors of the body, which seems to underlie the formation of resistance to infection caused by nonspecific microorganisms. | 1988 | 3046195 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| immunoreactivity of a surface wall fraction produced by spherules of coccidioides immitis. | the membranous spherule outer wall (sow) isolated from liquid cultures of coccidioides immitis has been shown to elicit reactivity with human anti-coccidioides antibody by immunofluorescence and the immunodiffusion-tube precipitin assay. the serologically reactive components were extracted from sow with the nonionic detergent n-octyl-beta-d-glucopyranoside (og). the og-soluble fraction of sow was shown to be reactive with immunoglobulin g in 25 serum samples from coccidioidomycosis patients by a ... | 1988 | 3047063 |
| [lipid peroxidation in the pathogenetic and clinical aspects of infectious diseases]. | | 1988 | 3047989 |
| blood-injury phobia: a review. | natural human uneasiness about blood, injury, or deformity sometimes becomes a specific phobia, which can lead to serious disability if vital medical procedures are refused. blood-injury phobia usually starts in childhood and is often familial. unlike other phobic cues, which cause persistent tachycardia, blood-injury phobic cues evoke an initial rise in heart rate followed by vasovagal bradycardia and, frequently, syncope. although blood-injury phobia may have an evolutionary, genetic, and phys ... | 1988 | 3048117 |
| deletion of the shiga toxin gene in a chlorate-resistant derivative of shigella dysenteriae type 1 that retains virulence. | we used a probe specific for detecting the structural-gene sequences of shiga toxin to analyze the genetic nature of toxin synthesis in mutant derivatives of shigella dysenteriae type 1. a chlorate-resistant (chl) mutant (725-78) of s. dysenteriae type 1 strain 3818t, which had retained virulence but had lost production of high levels of cytotoxic activity associated with shiga toxin synthesis, contained a complete deletion of the shiga toxin structural-gene sequences. these structural-gene sequ ... | 1988 | 3049838 |
| [pro-allergy role of infection. a component of the mode of reacting]. | no organism from the cradle lives germ-free or allergen-free. the modalities by which infections facilitate conditions of abnormal reactivity, in particular respiratory asthma and allergy to cow milk proteins, are examined. 216 asthmatic children and 50 infants with rotavirus enteritis have been considered. infections, besides representing stimuli directly projected on the immune system, also constitute factors which more generally influence the way of reacting. | 1988 | 3050902 |
| [differentiation of virulent strains of shigella and entero-invasive escherichia from their avirulent variants using modified indirect immunoenzyme analysis]. | the data obtained in this investigation confirm that the modified indirect enzyme immunoassay (eia) permits the differentiation of virulent bacteria of the genus shigella and enteroinvasive escherichia (group 1), regularly containing virulence plasmids with a molecular weight of 120-140 md, from their avirulent variants which have lost these plasmids (group 2). the ratio of the optic density (od) values of the positive control samples (the od of group 1) to the od values of the negative ones (th ... | 1988 | 3051808 |
| the persistence of shigella flexneri in the united states: increasing role of adult males. | the annual reported isolation rate of shigella flexneri decreased from 1964 to 1973, but has remained constant since then at 1 per 100,000. between 1975 and 1985, the median age of males from whom s. flexneri was isolated rose from 5 to 26 years. during this time, the isolation rate of s. flexneri rose more than five-fold among men, did not change in adult women, and decreased in children. by 1985, 23 per cent of reported s. flexneri isolates came from men aged 20-49. increased male homosexual t ... | 1988 | 3052115 |
| the epidemiology of shigellosis in israel. | analysis of shigella isolates referred for serotyping to the central shigella reference laboratory has revealed a slight predominance of s. sonnei (47%) over s. flexneri (44%) in israel. in female patients, the predominance of s. sonnei isolates was more accentuated (54 v. 37.5% for s. flexneri). on the other hand, an increased incidence of s. flexneri was observed in military personnel (59 v. 32% for s. sonnei), and even more so in the arab population (69 v. 19%). incidence peaked in summer (ju ... | 1988 | 3052334 |
| the ecology and pathogenicity of urease-producing bacteria in the urinary tract. | urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ... | 1988 | 3053050 |
| the ecology and pathogenicity of urease-producing bacteria in the urinary tract. | urease activity is a physiological function of many bacteria that enables these organisms to utilize urea as a source of nitrogen. the association of ureolytic bacteria with human or animal hosts varies widely from a commensal relationship as demonstrated with skin microflora, a symbiotic relationship in the gastrointestinal tract, to a pathogenic relationship in the urinary tract. since similar or identical species of bacteria such as staphylococcus aureus are found in all three environments, t ... | 1988 | 3053050 |
| shigella flexneri bacteraemia in post partum period. | | 1988 | 3053638 |
| [a case using plasmapheresis in treating infectious toxicosis in young infants]. | | 1988 | 3054796 |
| [stability of shigella flexneri attenuated strain 2a 77 ade- rif-r after cultivation in a fermenter and lyophilization]. | the method for the cultivation of s. flexneri attenuated strain 2a 77 ade- rifr in a fermenter with the use of casein fermentative broth containing 200-250 mg% of amino nitrogen has been developed. no changes in the properties of the initial strain have been found after its cultivation in a fermenter or after the lyophilization of the cultures grown in a fermenter. after cultivation in casein fermentative broth with 100 mg% of amino nitrogen the presence of s-r dissociation has been established, ... | 1988 | 3055763 |
| characterization of invasion plasmid antigen genes (ipabcd) from shigella flexneri. | the large invasion plasmid of shigella flexneri m9ot-w was used to generate recombinant plasmids carrying the ipaa, -b, -c, and -d genes, whose products are associated with the entry of the bacteria into colonic epithelial cells. complete dna sequences of ipab, -c, and -d were determined. the proteins predicted (62, 42, and 37 kda, respectively) from the nucleotide sequences lack a signal-peptide sequence. hydrophilic segments of the ipab and ipac proteins were found to overlap known epitopic do ... | 1988 | 3057506 |
| persistent shigellosis. | shigella usually causes a self-limited infection which untreated lasts on average seven days (range one to 30 days). we report a patient who had persistent symptoms for 10 months caused by shigella flexneri which was finally identified on cultures from a rectal swab. | 1988 | 3058558 |
| affinity chromatography of reca protein and reca nucleoprotein complexes on reca protein-agarose columns. | we have analyzed the nature of reca protein-reca protein interactions using an affinity column prepared by coupling reca protein to an agarose support. when radiolabeled soluble proteins from escherichia coli are applied to this column, only the labeled reca protein from the extract was selectively retained and bound tightly to the affinity column. efficient binding of purified 35s-labeled reca protein required mg2+, and high salt did not interfere with the binding of reca protein to the column. ... | 1988 | 3058701 |
| [etiologic structure of bacterial dysentery in the ussr in 1983-1985]. | the etiological structure of dysentery in the ussr in 1983-1985 is characterized. sonne dysentery was found to prevail in the territories with adequate water supply, while dysentery caused by shigella flexneri prevailed at the territories with unsatisfactory water supply. s. dysenteriae and s. boydii were found to play a limited role in the etiology of dysentery. in the presence of global pandemic, an increase in the isolation rate of s. dysenteriae i in the ussr is observed. the data on the bio ... | 1988 | 3061262 |
| a 13c-n.m.r. study of some shigella flexneri o-polysaccharides. | 13c-n.m.r. data for some shigella flexneri o-polysaccharides are reported. it is concluded that the chemical shifts observed for the linear shigella flexneri type y o-polysaccharide are similar to those calculated by adding substituent shifts, obtained from disaccharides with similar stereochemistry around the glycosidic linkage, to the chemical shifts for the monomers. the influence of substitution with alpha-d-glucopyranosyl and/or o-acetyl groups in different positions of this polysaccharide ... | 1988 | 3061647 |
| [pathogenetic significance of virulence-associated genes in shigella flexneri]. | | 1988 | 3063851 |
| interaction between vestibulocollic and cervicocollic reflexes: automatic compensation of reflex gain by muscle afferents. | | 1988 | 3064144 |
| [electron microscopic study of the interaction of virulent and avirulent shigella flexneri strains with the macrophages of murine peritoneal exudate]. | the electron microscopic dynamic study of the interaction of s. flexneri virulent strain 2a and s. flexneri avirulent strain, genetically related to strain 2a and having the same antigenic structure, with peritoneal macrophages of cba mice at an early period showed differences in the cytotoxic action of these strains. even at the early period of the interaction between macrophages and virulent shigellae structural changes were observed in macrophages: ruptures in the membranes of parasitophore p ... | 1988 | 3064506 |
| [scientific bases of epidemiologic surveillance of intestinal infections]. | | 1988 | 3064508 |
| arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics. | a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ... | 1988 | 3064807 |
| arginyl-trna synthetase from escherichia coli, purification by affinity chromatography, properties, and steady-state kinetics. | a blue sephadex g-150 affinity column adsorbs the arginyl-trna synthetase of escherichia coli k12 and purifies it with high efficiency. the relatively low enzyme content was conveniently purified by deae-cellulose chromatography, affinity chromatography, and fast protein liquid chromatography to a preparation with high activity capable of catalyzing the esterification of about 23,000 nmol of arginine to the cognate trna per milligram of enzyme within 1 min, at 37 degrees c, ph 7.4. the turnover ... | 1988 | 3064807 |
| serological response to plasmid-encoded antigens in children and adults with shigellosis. | the immunological response to plasmid-encoded antigens of virulent shigella was determined in thai children less than 4 yr of age and in thai adults by immunoblot analysis and elisa. forty-two percent (8/19) of thai children and 4% (1/22) of thai adults with shigellosis developed a greater than or equal to 4-fold rise in igg antibody titer to water-extracted antigens of shigella flexneri m90t by elisa (p = 0.006). two children and one lactating mother with shigellosis developed a 4-fold rise in ... | 1988 | 3066570 |
| regulation of urea cycle enzymes. | | 1988 | 3067149 |
| [characteristics of the course of acute dysentery in a 5-year-old child complicated 3 times by intestinal invagination]. | | 1988 | 3067205 |
| physiological and behavioral aspects of stress in adolescence. | gonadal and adrenal hormone levels appear to be linked to the stressors experienced by young adolescents. adjustment problems were accompanied by a profile of lower gonadal steroids and higher adrenal androgen levels, primarily androstenedione. later gonadal maturation may be a result of stress suppressing the reproductive axis. higher levels of androstenedione may be indicative of chronic levels of stress. however, the findings for androstenedione are complicated by the fact that androstenedion ... | 1988 | 3067560 |
| genetic relatedness of the basic replicon of the virulence plasmid in shigellae and enteroinvasive escherichia coli. | the regions required for replication (rep) and for stable maintenance (stb) of the 230 kilobase virulence plasmid, pmysh6000, in shigella flexneri 2a ysh6000 were identified on the restriction enzyme sa/l fragments, c and o, respectively. the stable plasmid, pmysh6610, consisting of adjacent fragments c and o was found to belong to the incompatibility group fl. in all strains of shigellae and enteroinvasive escherichia coli (eiec) tested, virulence plasmids were found to be highly homologous wit ... | 1988 | 3070262 |
| [vitamin d as an immuno-hematopoietic hormone. new perspectives on a previously known substance]. | 1,25-dihydroxyvitamin d3 (1.25[oh]2d3), the most active metabolite of vitamin d3, plays an important role in bone and mineral metabolism. there is growing evidence that the vitamin d endocrine system may be important in other systems as well: 1,25(oh)2d3 is synthesized in extrarenal tissue. macrophages from both normal human individuals and patients with sarcoidosis are capable of producing 1,25(oh)2d3. specific receptors for 1,25(oh)2d3 are present in a number of cells and tissues other than th ... | 1988 | 3070739 |
| [the importance of secondary immune deficiencies in the pathogenesis of dysentery]. | some immunity indices were studied in patients with acute dysentery. the number of total and active t-lymphocytes, t mu- and t gamma-cells, t-rabbit, t-staphylococcal, b-total and b-lymphocytes forming rosettes with mouse erythrocytes were determined for assessment of the immune status. changes in the above indices with relation to the severity of the disease were noted. suppression of the t- and b-systems of immunity were noticeable in patients with a severe and lingering course of disease. del ... | 1988 | 3070808 |
| [free-radical oxidation of blood plasma lipids in patients with acute intestinal infections]. | blood plasma free radical lipid oxidation was studied in 98 patients with acute dysentery (ad) and 64 patients with food toxinfection (fti). free radical lipid oxidation was assessed by the intensity of chemiluminescence and the level of lipid peroxides. activation of blood plasma lipid peroxidation was established with relation to a clinical variant of the disease. the effect of indomethacin on the parameters of free radical lipid oxidation was noted in patients with ad and fti. | 1988 | 3070810 |
| nucleotide sequence of the invasion plasmid antigen b and c genes (ipab and ipac) of shigella flexneri. | the nucleotide sequence of a 4.8 kilobase (kb) hindiii fragment from pwr100, the virulence plasmid of shigella flexneri 5, was determined and analysed. this fragment encodes polypeptides b (62 kilodalton, kd) and c (43 kd) which have already been described as two of the four immunogenic polypeptides of shigellae. the nucleotide sequence revealed that in addition to the ipab and ipac genes encoding polypeptides b and c, a third complete open reading frame was found within the fragment. the gene, ... | 1988 | 3071655 |
| chronic shigella infection in an hiv seropositive patient--ontario. | | 1988 | 3072101 |
| outbreak of shigella flexneri in a nursing home--alberta. | | 1988 | 3072103 |
| plasmid mediated drug resistance in shigella flexneri serotypes 1-6 during 1974-85. | | 1988 | 3072296 |
| [an analysis of distribution of shigella groups for thirty-seven years (1949-1985) in china]. | | 1988 | 3073017 |
| [evaluation of selective properties of mac conkey's medium enriched with potassium tellurite for growing shigella and yersinia strains]. | | 1988 | 3073281 |
| shigellemia in an infant with intractable diarrhea. | | 1988 | 3074059 |
| laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy. | | 1988 | 3074131 |
| laboratory studies on the mode of action and application of antiestrogen for breast cancer therapy. | | 1988 | 3074131 |
| [separation of shigella enterotoxin and immunosuppression factors]. | | 1988 | 3074243 |
| [detection and characterization of collagenolytic activity of serine proteases of the subtilisin type]. | | 1988 | 3074903 |
| [infantile shigella infections in region ix]. | | 1988 | 3075065 |
| effect of fermentation of ghanaian maize dough on the survival and proliferation of 4 strains of shigella flexneri. | fermented and non-fermented ghanaian maize dough was seeded with approximately 10(7) colony forming units of 4 strains of shigella flexneri which had been isolated from patients with dysentery. in the non-fermented maize dough (ph 6.2) the shigellae were detectable in large numbers for up to 24 h after exposure. in the maize dough that had been fermented for 3 d (ph 3.2) 3 strains were detectable in small numbers for up to 6 h after inoculation. thereafter none was isolated. the fourth strain, t ... | 1988 | 3076718 |
| antibiotic resistance among shigella species isolated in tehran, iran. | a study of adult patients with gastroenteritis was carried out over an 18-month period from march 1984 until august 1985, with the aim of detecting shigella species and studying their antibiotic resistance pattern. two thousand four hundred and eighteen stool specimens were examined and 230 isolations of shigella were made, a detection rate of 9.5%. of the 230 isolates, 61.2% were found to be sh. flexneri, and resistance to ampicillin, chloramphenicol, co-trimoxazole and tetracycline was extreme ... | 1988 | 3077023 |
| [beef-extract agar with sodium nucleinate as a medium for storage and stabilization of biological properties of shigella]. | | 1986 | 3078175 |
| [shigella serotypes and drug resistance patterns of strains isolated in etimesgut, turkey, in 1987]. | drug resistance patterns of shigella strains were investigated in a prospective manner in etimesgut district during a period of 1 year. thirty strains shigella were isolated, belonged to three subgroups with preponderance of sh. flexneri (70%), followed by sh. sonnei (27%) and sh. boydii (3%). the resistance was highest with streptomycin (80%), followed by trimethoprim-sulfamethoxazole (tmp-smz) (53%) and ampicillin (43%). only three strains (10%) were sensitive to all eight antibiotics tested. ... | 1988 | 3078794 |
| different classes of antibody activities to trichosporon cutaneum antigen in summer-type hypersensitivity pneumonitis by enzyme-linked immunosorbent assay. | to assess the diagnostic value of antibody activities to trichosporon cutaneum antigen in patients with summer-type hypersensitivity pneumonitis in japan, we measured the serum igg, iga, and igm antibodies to saline-soluble antigen of homogenized t. cutaneum cells by enzyme-linked immunosorbent assay (elisa). the igg antibody activity was observed in serum samples from 25 (83%) of the 30 patients and in 9 (82%) of the 11 asymptomatic family members. the iga antibody activity was found in 25 (83% ... | 1986 | 3079979 |
| different classes of antibody activities to trichosporon cutaneum antigen in summer-type hypersensitivity pneumonitis by enzyme-linked immunosorbent assay. | to assess the diagnostic value of antibody activities to trichosporon cutaneum antigen in patients with summer-type hypersensitivity pneumonitis in japan, we measured the serum igg, iga, and igm antibodies to saline-soluble antigen of homogenized t. cutaneum cells by enzyme-linked immunosorbent assay (elisa). the igg antibody activity was observed in serum samples from 25 (83%) of the 30 patients and in 9 (82%) of the 11 asymptomatic family members. the iga antibody activity was found in 25 (83% ... | 1986 | 3079979 |
| effect of guinea pig or monkey colonic mucus on shigella aggregation and invasion of hela cells by shigella flexneri 1b and 2a. | the effects of guinea pig and rhesus monkey colonic mucus preparations on shigella aggregation and invasion of hela cell monolayers by shigella flexneri serotype 1b, 2a, and 5 strains were investigated. guinea pig mucus caused agglutination of s. flexneri serotype 1b but not of s. flexneri serotype 2a or 5. guinea pig mucus also inhibited hela cell invasion by s. flexneri serotypes 1b and 2a. monkey mucus neither agglutinated any shigella strain nor inhibited hela cell invasion. | 1986 | 3081449 |
| characterization of the membrane-bound protein kinase c and its substrate proteins in canine cardiac sarcolemma. | cardiac sarcolemma was purified from canine ventricles. enrichment of the sarcolemmal membranes was demonstrated by the high (na+ + k+)-atpase activity of 28.0 +/- 1.5 mumol pi/mg protein per h and the high concentration of muscarinic receptors with the bmax of 8.2 +/- 2.5 pmol/mg protein as determined by [3h]qnb binding. the purified sarcolemma also contains significant levels of a membrane-bound ca2+ and phospholipid-dependent protein kinase (protein kinase c). to elucidate the protein kinase ... | 1986 | 3082370 |
| [use of bladder biopsy in the study of urinary schistosomiasis]. | it is common to find schistosoma eggs in urine, faeces, and biopsies from rectal mucus membranes presently in use. it is less common to observe adults in routine bladder biopsies. the authors report such a case and discuss the importance of the bladder biopsy to estimate tissular inflammatory response which could reflect the host-parasite balance. | 1986 | 3084108 |
| disialoganglioside gd2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin. | human melanoma cells (m21) actively attach and spread on a fibronectin substrate. indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside gd2, the major ganglioside expressed on m21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substrate. scanning and transmission immunoelectron microscopic studies with anti-gd2 monoclonal antibodies a ... | 1986 | 3084502 |
| disialoganglioside gd2 distributes preferentially into substrate-associated microprocesses on human melanoma cells during their attachment to fibronectin. | human melanoma cells (m21) actively attach and spread on a fibronectin substrate. indirect immunofluorescence assays with specific monoclonal antibodies directed to the disialoganglioside gd2, the major ganglioside expressed on m21 melanoma cells, indicate that during the cell attachment process this molecule redistributes into microprocesses that make direct contact with the fibronectin substrate. scanning and transmission immunoelectron microscopic studies with anti-gd2 monoclonal antibodies a ... | 1986 | 3084502 |
| endogenous luteinizing hormone surge in women during induction of multiple follicular development with pulsatile follicle stimulating hormone. | in this study nine consecutive normally cycling women undergoing in-vitro fertilization (ivf) were superovulated with clomiphene citrate followed by pulsatile 'pure' fsh injected s.c. via a pump (28 iu every 3 h). all women displayed an endogenous lh surge, which was markedly attenuated in most of the cases (peak value 44.5 +/- 5.9 u/l, duration 29.2 +/- 1.2 h, mean +/- sem) as compared to spontaneous cycles. an increase in serum progesterone levels before the onset of the lh surge was seen in o ... | 1986 | 3085995 |
| natural and recombinant interferons inhibit epithelial cell invasion by shigella spp. | the effect of natural and recombinant interferons (ifns) on the abilities of shigella flexneri, s. sonnei, and salmonella typhimurium to invade different human and murine cells was examined. pretreatment of cell monolayers with natural and recombinant ifns reduced the number of shigella-infected cells in a dose-dependent manner. establishment of an anti-invasive cellular state was time dependent, requiring 10 h for 50% inhibition of bacterial invasion. the inhibitory effect of ifn was species sp ... | 1986 | 3086234 |
| measurement of phospholipid turnover in cultured hormone responsive pituitary cells. | | 1986 | 3086663 |
| genetic basis for altered idiotype expression in the hyperimmune response to (4-hydroxy-3-nitrophenyl)acetyl hapten. | to assess the significance of somatic point mutation in the hyperimmune response to the hapten np, an in vivo enrichment procedure was followed. mice that expressed high titers of b1-8 idiotopic determinants were selected as donors for serial transfer of small numbers of immune spleen cells into syngeneic irradiated recipient mice. cells expressing b1-8 idiotopic determinants were chosen for enrichment because b1-8 cross-reactive determinants constitute a significant portion of the primary respo ... | 1986 | 3088116 |
| the effect of the beta-carboline fg 7142 on the behaviour of male rats in a living cage: an ethological analysis of social and nonsocial behaviour. | the effects of fg 7142, a beta-carboline benzodiazepine receptor partial inverse agonist, on the social behavior of pair-housed rats were investigated. four 6-min dyadic social encounters in a living cage were observed in a paradigm in which one member of a pair of rats was injected. the four injection groups (n = 8) were vehicle control, and fg 7142 at 2.5, 5.0 and 10.0 mg/kg, respectively. all injections were administered 2 min before the start of the first observation trial. compared to the e ... | 1986 | 3088637 |
| study of aldose reductase inhibition in intact lenses by 13c nuclear magnetic resonance spectroscopy. | carbon-13 nuclear magnetic resonance spectroscopy has been used in the study of glucose metabolism, specifically aldose reductase inhibition, in intact rabbit lenses maintained in organ culture. this technique provides an effective method of screening potential inhibitors of aldose reductase under conditions that more closely approximate in vivo conditions than do earlier methods. the aspirin substitutes acetaminophen and ibuprofen were studied as aldose reductase inhibitors and were found to be ... | 1986 | 3088727 |
| disopyramide phosphate effects on slow and depressed fast responses. | we studied the effects of disopyramide phosphate on explanted neonatal rat ventricle cells exhibiting depressed fast responses or naturally occurring slow response action potentials together with automatic activity. disopyramide suppressed the spontaneous activity at a concentration of 2.5 micrograms/ml with a half-maximal value of 10 micrograms/ml. before spontaneous activity was lost, there was an increase in beating rate possibly related to membrane depolarization. in depressed fast and slow ... | 1986 | 3089566 |
| [cell proliferation in the carcinogenetic processes of tongue carcinoma induced by 4-nitroquinoline-1-oxide in rats. 1. relation between morphology and microvascular architecture of the filiform papillae of dorsal surface of the tongue in normal albino rats]. | | 1986 | 3090703 |
| chloroalanyl antibiotic peptides: antagonism of their antimicrobial effects by l-alanine and l-alanyl peptides in gram-negative bacteria. | a large number of structurally diverse di- and tripeptides containing the alanine racemase inactivator beta-chloro-l-alanine (beta-cl-lala) have been synthesized, and their antibacterial properties in vitro have been evaluated. the dipeptides 1, 3-6, and 8-17 and the tripeptide 20 are all broad-spectrum antibacterial agents with considerable potency against both gram-positive and gram-negative species, but none of these peptides improves dramatically on the antibiotic efficacy of the previously ... | 1986 | 3093682 |
| noradrenergic and opioid mediation of tricyclic-induced reversal of escape deficits caused by inescapable shock pretreatment in rats. | the present study was undertaken to investigate the possible involvement of a noradrenergic and opioid mediation of the reversal by tricyclic antidepressants of escape deficits produced by inescapable shock pretreatment. rats were first exposed to 60 inescapable shocks (15 s duration, 0.8 ma, every min +/- 15 s) and 48 h later subjected, to daily shuttle-box sessions (30 trials/day, iti: 30 s) during 3 consecutive days. twice-daily ip injection of desipramine or clomipramine (total daily dose: 3 ... | 1986 | 3094068 |
| [prevalence of campylobacter jejuni complement fixing antibody in healthy people]. | | 1986 | 3097197 |
| rash mutants deficient in gtp binding. | single amino acid substitutions were introduced into a region of the rash protein (residues 116, 117, and 119) homologous to a variety of diverse gtp-binding proteins. each of the mutant p21 proteins displayed a significant reduction (10- to 5,000-fold) in gtp binding affinity. activated rash proteins deficient in gtp binding were unaltered in their ability to morphologically transform nih 3t3 cells. | 1986 | 3097518 |
| signals in the phi 29 dna-terminal protein template for the initiation of phage phi 29 dna replication. | the protein-free terminal fragments hindiii b and l, from the left and right ends of phi 29 dna, respectively, but not internal fragments of similar size, were active as templates in the formation of the p3-damp initiation complex in an in vitro system containing purified phi 29 terminal protein p3 and dna polymerase p2, although the activity was lower than that obtained with the phi 29 dna-p3 complex. these results indicate the existence of specific sequences at the ends of phi 29 dna that allo ... | 1986 | 3097958 |
| radioimmunoscintigraphy of human gastric carcinoma xenografts with 125i-labeled monoclonal antibody. | a monoclonal antibody (gc 302) established in our laboratory, which was reactive with gastric carcinoma and other epithelial carcinoma but not with normal gastric mucosa or other malignant tumors of mesenchymal origin, was used to investigate the radioimmunolocalization of tumors. various kinds of target cells (5 x 10(5)) were incubated with 125i-labeled gc 302, and radioactivity was determined with a gamma counter. it was shown that there was a 500- to 1,000-fold increase in counts for gastric ... | 1986 | 3098721 |
| characterization and diagnostic application of a lipopolysaccharide core oligosaccharide-protein conjugate. | shigella sonnei phase ii core oligosaccharide (osphii) was linked covalently with protein (bovine serum albumin or tetanus toxoid) to obtain a conjugate (osphii-protein) of good immunogenicity in rabbits. anti-osphii-protein conjugate sera contained high levels of immunoglobulin g antibodies against the complete core region of sh. sonnei lipopolysaccharide. the antigenic relationships between lipopolysaccharides of r1, r3 and r4 core types using anti-osphii-protein sera were studied. these antis ... | 1986 | 3098854 |
| effect of measles-virus infection and interferon treatment on invasiveness of shigella flexneri in hep2-cell cultures. | the influence of measles-virus infection on the invasiveness of shigella flexneri in hep2-cell cultures was studied. bacterial invasiveness was significantly enhanced in cell cultures incubated with virus before bacterial inoculation. this effect was a function of time after introduction of virus to the cell cultures and of the concentration of virus. the increase in bacterial invasiveness was observed before production of infectious virus particles and before a cytopathic effect was evident. a ... | 1986 | 3098977 |
| separation and quantification of prostaglandins e1 and e2 as their panacyl derivatives using reverse phase high pressure liquid chromatography. | separation and quantification of prostaglandin e1 (pge1) and prostaglandin e2 (pge2) were achieved using reverse phase high performance liquid chromatography (hplc). panacyl bromide (p-(9-anthroyloxy)phenacyl bromide) (pab) derivatives of pge2 and pge1 were prepared. reverse phase hplc using a linear gradient of 56% to 80% acetonitrile in water containing 0.10% acetic acid gave baseline resolution of the two derivatives. a 3 um diameter particle, c18 column provided good resolution and reproduci ... | 1986 | 3099332 |
| a strain of multiform cryptococcus neoformans. | | 1986 | 3100152 |
| on the mechanism of antithrombotic action of flavonoids. | flavonols (quercetin and rutin) and flavanes (cyanidol and meciadonol) were studied for their effect on non-enzymatic lipid peroxidation, lipoxygenase and cyclo-oxygenase activities, binding to albumin and platelet membranes. these biochemical properties of four flavonoids were compared with respect to their antithrombotic action in vivo and their efficacy at influencing the platelet-endothelium interaction in vitro. all four flavonoids inhibited the ascorbate-stimulated formation of malondialde ... | 1987 | 3101704 |
| salicylates inhibit paf-acether-induced rat paw oedema when cyclooxygenase inhibitors are ineffective. | the cyclooxygenase inhibitors indomethacin, piroxicam, ibuprofen, naproxen and flurbiprofen failed to block rat paw oedema induced by paf-acether, whereas aspirin and sodium salicylate were effective. two mixed cyclooxygenase and lipoxygenase inhibitors ndga, bw 755c and dexamethasone reduced oedema in a dose - dependently. the selective paf-acether antagonist, bn 52021, was effective against paf-acether at 5 - 20 mg/kg. the lipoxygenase derivates may be involved in paw oedema induced by paf-ace ... | 1986 | 3103172 |
| coagulation defects of aflatoxin intoxicated rabbits. | twelve new zealand white rabbits were intoxicated with aflatoxin b1. most rabbits developed a coagulation defect near the time of death. immediately prior to death there were significant decreases in factors v, vii, and viii coagulant activities and fibrinogen concentration without a change in plasma fibrin(ogen) degradation product concentration, platelet number, and detectable plasma fibrin monomers. microscopic evidence of disseminated intravascular coagulation was present in one rabbit with ... | 1987 | 3103313 |
| characterization of self-transmissible plasmids determining lactose fermentation and multiple antibiotic resistance in clinical strains of klebsiella pneumoniae. | the lactose fermentation (lac+) and antibiotic resistance (r+) phenotypes were conjugally transferred from klebsiella pneumoniae strains (k166, k182, k186, k218, and k220) to salmonella typhi, s. typhimurium, shigella flexneri, and vibrio cholerae. the genes for lactose fermentation and antibiotic resistance were located on the plasmids. further analysis of plasmid dna from these isolates indicated the presence of multiple plasmids (mr ranged less than 2.7 to 70 x 10(6)). the lac+r+ plasmids p16 ... | 1987 | 3107002 |
| osteonectin content in human osteogenesis imperfecta bone shows a range similar to that of two bovine models of oi. | samples of human osteogenesis imperfecta (oi) bone were analyzed for osteonectin content by sds gel electrophoresis and immunodetection on western blots. the oi bone osteonectin content varied from normal to severely depressed. previously, we showed that two clinically identical but genetically unrelated bovine models of oi were differentiated biochemically by their bone osteonectin content: one oi model had normal bone osteonectin while the other was severely depressed in this parameter. the da ... | 1987 | 3107778 |
| in vivo studies on intracellular ph, focal flow, and vessel diameter in the cat cerebral cortex: effects of altered co2 and electrical stimulation. | the time course of changes in cortical tissue ph (phi) and blood flow during cortical seizures in halothane-anesthetized cats was examined. the clearance of the molecular form of umbelliferone (um) was used to estimate focal cortical blood flow (cbfu), whereas the ratio of the molecular to the ionic form of the molecule was used to concurrently calculate the local phi. resting phi and flow in normocarbic animals was 7.116 +/- 0.008 and 46 +/- 8 ml/100 g/min, respectively. respiratory induced alt ... | 1987 | 3108271 |
| [influence of bcg in the control of humoral deficiency induced by mastocytoma p-815]. | viable bcg bacilli, in a lipid emulsion, inoculated intravenously, were capable of reverting the profound humoral immunosuppression induced in adult dba/2 mice by the mastocytoma p-815. bcg increased not only the number of hemolytic plaque forming cells, but also the serum titers of hemagglutinating igm antibodies. however, no blocking effect was detected on normal tumor progression. | 1986 | 3108617 |
| pulmonary clearance of pseudomonas aeruginosa in neutropenic mice. effects of systemic immunization. | systemic immunization with killed pseudomonas improves pulmonary clearance of these bacteria in normal mice. to test the effects of active systemic immunization on early pulmonary clearance of pseudomonas in neutropenic mice, balb/c mice were intraperitoneally immunized with 10(8) pseudomonas or injected with saline twice weekly for 2 wk. ten days after the last immunization, immunized and control mice received tail vein injections with either nitrogen mustard or saline daily for 3 days. two day ... | 1987 | 3109290 |
| inner-city survey for tuberculosis: evaluation of diagnostic methods. | a total of 1,271 persons living in a socially and economically depressed, inner-city area of vancouver, british columbia, voluntarily attended a tuberculosis case-finding campaign. chest x-ray, on-the-spot specimen of sputum, and tuberculin skin test were offered at the time of the first attendance. all 3 diagnostic methods were found to be well accepted, with 93% of the participants having an x-ray, over 95% producing a sputum specimen, and almost 95% having a tuberculin test (a quarter of thes ... | 1987 | 3109292 |
| effects in rats of dietary protein inadequacy on lactose production, milk volume and components of the lactose synthetase complex (ec 2.4.1.22). | lactose synthetase (ls) is a complex of alpha-lactalbumin and n-acetylglucosamine galactosyl transferase (gt). ls is the rate-limiting enzyme in lactose biosynthesis. lactose is the main osmotic component of milk and may thereby affect milk volume. dietary protein inadequacy reduces milk volume and lactose content. the study investigates the role of ls activity in these phenomena. lactating rats were fed adequate and inadequate amounts of protein of high (milk protein) and low (cereal protein) q ... | 1987 | 3109312 |
| purification and characterization of dihydrofolate reductase from soybean seedlings. | dihydrofolate reductase (dhfr; ec 1.5.1.3) was purified to homogeneity from soybean seedlings by affinity chromatography on methotrexate-aminohexyl sepharose, gel filtration on ultrogel aca-54, and blue sepharose chromatography. sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the enzyme gave a single protein band corresponding to a molecular weight of 22,000. the enzyme is not a 140,000 da heteropolymer as reported by others. amino acid sequence-specific antibodies to intact human d ... | 1987 | 3109322 |
| studies on the rate-determining factor in testosterone hydroxylation by rat liver microsomal cytochrome p-450: evidence against cytochrome p-450 isozyme:isozyme interactions. | the aim of the present study was to examine a recent proposal that inhibitory isozyme:isozyme interactions explain why membrane-bound isozymes of rat liver microsomal cytochrome p-450 exert only a fraction of the catalytic activity they express when purified and reconstituted with saturating amounts of nadph-cytochrome p-450 reductase and optimal amounts of dilauroylphosphatidylcholine. the different pathways of testosterone hydroxylation catalyzed by cytochromes p-450a (7 alpha-hydroxylation), ... | 1987 | 3109324 |
| immunological relationship among glycogen-protein complexes of different species. | glycogen-protein complexes from the muscles of celerio euphorbiae and horse as well as from the livers of frog, carp and man were isolated and used as antigens. after immunization of rabbits antisera were obtained from which fractions of immunoglobulins were isolated. antibodies were used in agar immunodiffusion tests and in quantitative reaction of immunoprecipitation with glycogens. the immunoprecipitation test proved the occurrence of quantitative differences in cross reaction of complexes wi ... | 1986 | 3109350 |
| recombinant human tissue-type plasminogen activator therapy in acute thromboembolic stroke. | systemic fibrinolytic therapy for acute stroke is no longer recommended because of resulting systemic fibrinolysis and the risk of intracerebral hemorrhage. human tissue-type plasminogen activator (tpa) is a native enzyme that converts plasminogen to plasmin with subsequent clot lysis. the affinity for plasminogen is increased several-fold when the substrate is bound to fibrin. at appropriate dosage, "clot-specific" thrombolysis may be achieved at the surface of the thrombus without creating sys ... | 1987 | 3112328 |
| histological changes following transplantation of developing teeth to more advanced functional positions. | | 1987 | 3113409 |
| efficacy of a polyvalent protein-polysaccharide dysentery oral vaccine. | | 1987 | 3115716 |
| investigation of a health care worker with symptomatic human immunodeficiency virus infection: an epidemiologic approach. | | 1987 | 3116462 |
| the inhibition by valproic acid of the mitochondrial oxidation of monocarboxylic and omega-hydroxymonocarboxylic acids: possible implications for the metabolism of gamma-aminobutyric acid. | the interactions of 1-5 mm valproic acid with the hepatic fatty acid oxidation are here described. valproic acid was not substrate for hepatic peroxisomal fatty acid oxidation. its activation outside the mitochondrial matrix compartment was poor when compared to that of octanoic acid, a fatty acid containing the same number of carbones. valproic acid did not inhibit the fatty acyl-coa oxidase nor the cyanide-insensitive acyl-coa oxidation. valproic acid inhibited the mitochondrial oxidations of ... | 1987 | 3117781 |