| preparation of polyclonal antibody specific for atplc4, an arabidopsis phosphatidylinositol-specific phospholipase c in rabbits. | phosphoinositide-specific phospholipase cs (pi-plcs) are important enzymes in eukaryotes, which catalyze the hydrolysis of phosphatidylinositol 4,5-bisphosphate into the two second messengers inositol 1,4,5-trisphosphate and diacylglycerol. the arabidopsis genome contains nine putative pi-plc genes. atplc4, an abiotic stress induced gene, has been reported to encode an active pi-plc isoform. however, the exact roles of putative atplc4 in plant remain to be elicited. the first 108 amino acid resi ... | 2007 | 17142056 |
| rapid detection of escherichia coli by using antibody-conjugated silver nanoshells. | silver shells of 20 nm thickness have been deposited on silica particles of 200 nm diameter with narrow size distribution. silver nanoshells dispersed in water exhibit a strong surface plasmon resonance band at 443 nm. this band was found to be very sensitive to rabbit immunoglobulin g antibodies, which were anchored on the nanoshells. these in turn could be utilized to detect the presence of small (approximately 5) to large numbers (approximately 10(9)) of escherichia coli in water. the protoco ... | 2006 | 17193045 |
| maize beta-glucosidase-aggregating factor is a polyspecific jacalin-related chimeric lectin, and its lectin domain is responsible for beta-glucosidase aggregation. | in certain maize genotypes, called "null," beta-glucosidase does not enter gels and therefore cannot be detected on zymograms after electrophoresis. such genotypes were originally thought to be homozygous for a null allele at the glu1 gene and thus devoid of enzyme. we have shown that a beta-glucosidase-aggregating factor (bgaf) is responsible for the "null" phenotype. bgaf is a chimeric protein consisting of two distinct domains: the disease response or "dirigent" domain and the jacalin-related ... | 2007 | 17210577 |
| lee-encoded regulator (ler) mutants elicit serotype-specific protection, but not cross protection, against attaching and effacing e. coli strains. | we previously showed that single dose orogastric immunization with an attenuated regulatory lee-encoded regulator (ler) mutant of the rabbit enteropathogenic escherichia coli (repec) strain e22 (o103:h2) protected rabbits from fatal infection with the highly virulent parent strain. in the current study we assessed the degree of homologous (serotype-specific) and heterologous (cross-serotype) protection induced by immunization with repec ler mutant strains of differing serotypes, or with a protot ... | 2007 | 17234305 |
| invariant ser211 is involved in the catalysis of pd-l4, type i rip from phytolacca dioica leaves. | multiple sequence alignment analysis of ribosome inactivating proteins (rips) has revealed the occurrence of an invariant seryl residue in proximity of the catalytic tryptophan. the involvement of this seryl residue in the catalytic mechanism of rips was investigated by site-directed mutagenesis in pd-l4, type 1 rip isolated from phytolacca dioica leaves. we show that the replacement of ser211 with ala apparently does not influence the n-beta-glycosidase activity on ribosomes (determined as ic(5 ... | 2007 | 17243169 |
| effect of carbon dioxide pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model. | to evaluate the effect of artificial co2 pneumoretroperitoneum on bacterial translocation in an experimental retroperitoneoscopy model. | 2007 | 17263621 |
| expression and antibody preparation of pou transcription factor qbrn-1. | the transcription factor brn-1, which belongs to pou-domain family, has been shown to play critical roles in the development of the nervous system. a cdna clone coding for a quail brn-1 homologue, qbrn-1, was isolated. to investigate whether this gene plays a role in the development of the quail nervous system, an anti-n-terminal peptide antibody was prepared. the coding region for amino acids 1-79 (the n-terminal domain of qbrn-1) was subcloned into trx fusion expression vector pet32c and intro ... | 2007 | 17266644 |
| cloning, sequencing, expression, and antigenic characterization of rmsp4 from anaplasma marginale isolated from paraná state, brazil. | anaplasmosis is a bovine intraerythrocytic disease caused by the bacterium anaplasma marginale; it causes significant economic losses in tropical and subtropical regions, worldwide. the msp4 gene of an a. marginale strain isolated in paran , brazil, was amplified by pcr and sequenced; its cloning into the pet102/d-topo vector produced an msp4-6xhis-v5-hp thioredoxin fusion gene construct. this recombinant clone was over-expressed in escherichia coli bl21(de-3); the expressed fusion protein was f ... | 2007 | 17278086 |
| recombinant production and properties of binding of the full set of mouse secreted phospholipases a2 to the mouse m-type receptor. | to date, 12 secreted phospholipases a2 (spla2s) have been identified in the mouse species and divided into three structural collections (i/ii/v/x, iii, and xii). on the basis of their different molecular properties and tissue distributions, each spla2 is likely to exert distinct functions by acting as an enzyme or ligand for specific soluble proteins or receptors, among which the m-type receptor is the best-characterized target. here, we present the properties of binding of the full set of mouse ... | 2007 | 17279628 |
| two novel dermonecrotic toxins lirecdt4 and lirecdt5 from brown spider (loxosceles intermedia) venom: from cloning to functional characterization. | loxoscelism (the condition produced by the bite of brown spiders) has been reported worldwide, but especially in warmer regions. clinical manifestations include skin necrosis with gravitational spreading while systemic loxoscelism may include renal failure, hemolysis and thrombocytopenia. the venom contains several toxins, of which the best biochemically and biologically studied is the dermonecrotic toxin, a phospholipase-d. purified toxin induces cutaneous and systemic loxoscelism, especially n ... | 2007 | 17296256 |
| clonorchis sinensis: molecular cloning, enzymatic activity, and localization of yolk ferritin. | ferritin is an intracellular protein that is involved in iron metabolism. a cdna clone of clonorchis sinensis (csftn), 565 bp long, encoded a putative polypeptide of 166 amino acids. csftn cdna revealed a putative loop-stem structure similar to iron-responsive element (ire). csftn polypeptide appeared homologous to the ferritin of trematodes with high sequential identity. phylogenetic tree analysis showed that csftn clustered with the ferritins of other flukes. recombinant csftn protein was prod ... | 2006 | 17304806 |
| [escherichia coli o157 in dutch domesticated rabbits]. | enterohaemorrhagic escherichia coli (ehec) has been detected in both wild and domesticated rabbits in other countries. the aim of this study was to determine whether the most pathogenic e. coli serotype, o157:h7, occurs in the dutch domesticated rabbit population and thus could form a public health risk. to this end, faecal samples were collected from rabbits from two rabbit farms and 741 rabbits of different breeds and origin and analysed for e. coli o157:h7, using a combination of enrichment, ... | 2007 | 17334149 |
| development of a simple latex agglutination assay for detection of shiga toxin-producing escherichia coli (stec) by using polyclonal antibody against stec. | rabbit antiserum raised against the whole-cell antigen of shiga toxin-producing escherichia coli (stec) strain vt3 (stx1+ stx2+ eae+) was repeatedly adsorbed with heat-killed cells of different non-stec strains and other enteric bacteria. thus, the antiserum obtained was designated vt3 antiserum. vt3 antiserum reacted with intimin type gamma. we assessed the reactivity of vt3 antiserum to whole-cell lysates of 87 strains of e. coli and other enteric bacteria by immunoblotting. the antiserum reco ... | 2007 | 17344348 |
| maternal milk contains antimicrobial factors that protect young rabbits from enteropathogenic escherichia coli infection. | enteropathogenic escherichia coli (epec) colibacillosis represents a major cause of lethal diarrhea in young children in developing countries. epec strains also infect numerous mammal species and represent a major economical problem in rabbit industry. protection against this pathogen is a challenging goal both in humans and in other mammal species. despite a good knowledge of the pathogenicity mechanisms of epec, the intrinsic and environmental factors that control the expression of epec virule ... | 2007 | 17344351 |
| real-time kinetics of discontinuous and highly conformational metal-ion binding sites of prion protein. | the prion protein (prp) is a metalloprotein with an unstructured region covering residues 60-91 that bind two to six cu(ii) ions cooperatively. cu can bind to prp regions c-terminally to the octarepeat region involving residues his111 and/or his96. in addition to cu(ii), prp binds zn(ii), mn(ii) and ni(ii) with binding constants several orders of magnitudes lower than those determined for cu. we used for the first time surface plasmon resonance (spr) analysis to dissect metal binding to specific ... | 2007 | 17345106 |
| endotoxin augments hemodynamic and metabolic effects of formyl-methionyl-leucyl-phenylalanine (fmlp) in rabbits. | the chemotactic factor formyl-methionyl-leucyl-phenylalanine (fmlp) when injected in rabbits causes dose-dependent transient hypotension, as well as neutropenia, thrombocytopenia and a decrease in systemic vascular resistance, as previously shown. since both fmlp and endotoxin are elaborated at sites of infection by certain bacteria, whether they act in concert to produce shock was examined. animals were pretreated with 380 microg/kg of e. coli endotoxin, 24 hours before the infusion of 10(-9) m ... | 2007 | 17354618 |
| the effect of mutations in alpha-tropomyosin (e40k and e54k) that cause familial dilated cardiomyopathy on the regulatory mechanism of cardiac muscle thin filaments. | e40k and e54k mutations in alpha-tropomyosin cause inherited dilated cardiomyopathy. previously we showed, using ala-ser alpha-tropomyosin (as-alpha-tm) expressed in escherichia coli, that both mutations decrease ca(2+) sensitivity. e40k also reduces v(max) of actin-tm-activated s-1 atpase by 18%. we investigated cooperative allosteric regulation by native tm, as-alpha-tm, and the two dilated cardiomyopathy-causing mutants. as-alpha-tm has a lower cooperative unit size (6.5) than native alpha-tr ... | 2007 | 17360712 |
| leishmania mexicana: molecular cloning and characterization of enolase. | the gene of leishmania mexicana enolase was cloned and overexpressed in escherichia coli as an active enzyme; the protein was biochemically analyzed. this enolase shares with enolases from other trypanosomatids the presence of three atypical residues, each with a reactive side group, near the active site, already described for the enzyme from trypanosoma brucei. the natural enzyme was purified, using a three-step procedure, from a cytosolic fraction of l. mexicana promastigotes. the kinetic prop ... | 2007 | 17382932 |
| extracellular rna constitutes a natural procoagulant cofactor in blood coagulation. | upon vascular injury, locally controlled haemostasis prevents life-threatening blood loss and ensures wound healing. intracellular material derived from damaged cells at these sites will become exposed to blood components and could contribute to blood coagulation and pathological thrombus formation. so far, the functional and mechanistic consequences of this concept are not understood. here, we present in vivo and in vitro evidence that different forms of eukaryotic and prokaryotic rna serve as ... | 2007 | 17405864 |
| ofloxacin-delivery system of a polyanhydride and polylactide blend used in the treatment of bone infection. | we developed a local drug-release system consisting of two biodegradable polymers, poly(sebacic anhydride) (psa) and poly-d,l-lactide (pla), for the treatment of chronic osteomyelitis. psa and pla were dissolved and blended at different ratios in tetrahydrofuran. ofloxacin was loaded with an 8:1 weight ratio of the blend to the drug. the ofloxacin-containing beads of the psa/pla blend were made by preheating and compressing them in a mold. the in vitro drug release showed that changing the ratio ... | 2007 | 17410574 |
| the enteritis complex in domestic rabbits: a field study. | a study of the causative agents of enteritis in domestic rabbits from 44 different accessions is described. in descending order of frequency, the organisms most commonly demonstrated were intestinal and hepatic coccidia (eimeria species), escherichia coli, clostridium spp., salmonella, bacillus piliformis, and rotavirus. the species of eimeria identified included those moderately pathogenic and coccidia of low pathogenicity. using seven antisera against known enterpathogenic strains of e. coli, ... | 1993 | 17424177 |
| protection against escherichia coli infection by antibody to the staphylococcus aureus poly-n-acetylglucosamine surface polysaccharide. | poly-n-acetylglucosamine (pnag) is a surface polysaccharide produced by staphylococcus aureus and staphylococcus epidermidis and is an effective target for opsonic and protective ab for these two organisms. recently, it has been found that escherichia coli produces an exo-polysaccharide, designated polyglucosamine, that is biochemically indistinguishable from pnag. we analyzed 30 e. coli strains isolated from urinary tract and neonatal bloodstream infections for the pga locus, pnag antigen produ ... | 2007 | 17446272 |
| cloning, expression and protective immunity evaluation of the full-length cdna encoding succinate dehydrogenase iron-sulfur protein of schistosoma japonicum. | 1071-bp fragment was obtained from the schistosoma japonicum (chinese strain) adult cdna library after the 3' and 5' ends of the incomplete expression sequence tag (est) of succinate dehydrogenase iron-sulfur protein of schistosoma japonicum (sjsdisp) were amplified by the anchored pcr with 2 pairs of primers designed according to the est of sjsdisp and the sequence of multiclone sites of the library vector. sequence analysis indicated that the fragment was a full-length cdna with a complete ope ... | 2007 | 17447029 |
| human trp14 gene homologue from amphioxus branchiostoma belcheri: identification, evolution, expression and functional characterization. | thioredoxin-related protein of 14 kda, trp14, has previously been identified only in humans. here we report the identification and expression of an amphioxus trp14 gene, named amphitrp14, the first such data in a non-mammalian organism. amphitrp14 consists of a 372-bp open reading frame coding for a 123-amino-acid protein with a calculated molecular weight of 14 kda. it shares 56% identity with human trp14 and possesses a highly conserved motif cpdc. sequence comparison suggests the evolutionary ... | 2007 | 17451532 |
| multiple and stepwise interactions between coatomer and adp-ribosylation factor-1 (arf1)-gtp. | the small gtpase adp-ribosylation factor-1 (arf1) plays a key role in the formation of coat protein i (cop i)-coated vesicles. upon recruitment to the donor golgi membrane by interaction with dimeric p24 proteins, arf1's gdp is exchanged for gtp. arf1-gtp then dissociates from p24, and together with other golgi membrane proteins, it recruits coatomer, the heptameric coat protein complex of cop i vesicles, from the cytosol. in this process, arf1 was shown to specifically interact with the coatome ... | 2007 | 17451557 |
| prokaryotic expression, purification, and sub-cellular localization of a novel alcohol acyltransferase from apple. | the coding region of the alcohol acyltransferase gene (mdaat2) from apple was sub-cloned into expression vectors, pet30a and pet32a, and introduced into e. coli for expression. the purified pet30a/mdaat2 fusion proteins were used to immunize rabbits following standard protocols. the partially soluble fusion proteins had alcohol acyltransferase activity and were detected only in the pet32a/origami b(de3) expression system. immunolocalization analysis indicated that mdaat2 is mainly in the cytopla ... | 2007 | 17479219 |
| high-efficiency recovery of target cells using improved yeast display system for detection of protein-protein interactions. | we constructed a high-throughput screening (hts) system for target cells based on the detection of protein-protein interactions by flow cytometric sorting due to the improvement in the yeast cell surface display system. interaction model proteins, which are the zz domain derived from staphylococcus aureus and the fc part of human immunoglobulin g (igg), were displayed on the yeast cell surface. we achieved a rapid and enhanced expression of these proteins as a result of adopting an appropriate y ... | 2007 | 17503034 |
| high-level expression, polyclonal antibody preparation and sub-cellular localization analysis of mouse rhox5 protein. | mouse reproductive homeobox on the x chromosome (rhox) is a novel homeobox gene cluster. rhox5, also called pem, belongs to the beta subcluster of rhox. codon analysis indicated that the cdna contains 16% of codons rarely used in escherichia coli. to achieve high-level expression of rhox5, the coding sequence of rhox5 was amplified and subcloned into the prokaryotic expression vector pet22b (+) in order to produce 6his-tagged fusion protein in the modified bl21 (de3) cells, namely rosetta2 (de3) ... | 2007 | 17509893 |
| influence of ph on antimicrobial activity of organic acids against rabbit enteropathogenic strain of escherichia coli. | susceptibility of the rabbit enteropathogenic strain escherichia coli c6 (o128 serogroup) to c6-c14 fatty acids, oleic, citric, lactic and fumaric acid at 5 mg/ml was determined by the plating technique in the near-neutral ph region (ph approximately 6.5), and in a weakly acid and acid environment (ph 5.4 +/- 0.1 and 2.2-2.5, respectively). in the near-neutral ph region caproic and caprylic acid reduced the concentration of viable cells by 3 and 6 orders, respectively. at lower ph the bactericid ... | 2007 | 17571799 |
| [cloning, expression and characterization of a gene encoding signal transduction protein wnt4 of schistosoma japonicum]. | wnt proteins together with their downstream effectors forms a set of important signal pathways. the wnt signal pathway is important in a wide variety of development processes including cell growth, cell differentiation, cell polarity and apoptosis. wnt4 is a key regulator of gonadal differentiation in humans and mice, playing a pivotal role in early embryogenesis. with race technique based on a est identified in our lab, a novel gene including a complete open reading frame was cloned and named s ... | 2007 | 17577981 |
| fada from fusobacterium nucleatum utilizes both secreted and nonsecreted forms for functional oligomerization for attachment and invasion of host cells. | fusobacterium nucleatum is a gram-negative anaerobe associated with various human infections, including periodontal diseases and preterm birth. a novel fada adhesin was recently identified for host-cell binding. it consists of 129 amino acid residues, with an 18-amino acid signal peptide. expression of fada in escherichia coli enhanced bacterial binding to host epithelial and endothelial cells. in both e. coli and f. nucleatum, fada exists in two forms, the intact pre-fada and the secreted matur ... | 2007 | 17588948 |
| prokaryotic expression, purification, and production of polyclonal antibody against human polypeptide n-acetylgalactosaminyltransferase 14. | polypeptide n-acetylgalactosaminyltransferase 14 (galnac-t14, ec 2.4.1.41) belongs to a large subfamily of glycosyltransferases residing in the golgi apparatus. n-acetylgalactosaminyltransferases (galnac-tases) catalyze the first step in the o-glycosylation of mammalian proteins by transferring n-acetyl-d-galactosamine (galnac) to peptide substrates. here, the cloning, expression, purification, and polyclonal antibody preparation of galnac-t14 were described. a full-length galnac-t14 cdna was in ... | 2007 | 17596962 |
| generation of antibodies against dmrt1 and dmrt4 of oreochromis aurea and analysis of their expression profile in oreochromis aurea tissues. | sex determination is composed of somatic and germ-line sex differentiation hierarchies whose interaction is poorly understood. a single gene known to control somatic sex determination, the dm-domain containing (doublesex/mab-3 dna-binding motif) gene, is highly conserved across species. vertebrate dmrt1 (dm-related transcription factor 1) expression occurs predominantly in the testis. here, however, isolated two distinct dm-domain cdna from oreochromis aurea ovary and testis have been named dmrt ... | 2007 | 17601609 |
| final report on the safety assessment of basic blue 99. | basic blue 99 is a direct, nonoxidative hair colorant used in temporary and semipermanent hair dyes. according to current reported usage data, basic blue 99 is used at concentrations from 0.004% to 2% and the most often reported use is in hair tints. hair dyes containing basic blue 99, as "coal tar" hair dye products, are exempt from the principal adulteration provision and from the color additive provision of the federal food, drug, and cosmetic act of 1938 when the label bears a caution statem ... | 2007 | 17613131 |
| cloning and characterization of a caspase gene from black tiger shrimp (penaeus monodon)-infected with white spot syndrome virus (wssv). | a black tiger shrimp (penaeus monodon) caspase cdna homologue (pmcasp) has been identified from a hemocyte library using a previously identified caspase homologue from the banana shrimp (penaeus merguiensis) as a probe. the full-length pmcasp was 1202bp with a 954bp open reading frame, encoding 317 amino acids. the deduced protein contained a potential active site (qacrg pentapeptide) conserved in most caspases. it had 83% identity with caspase of p. merguiensis and 30% identity with drice prote ... | 2007 | 17617486 |
| functional consequences of sequence variation in bundlin, the enteropathogenic escherichia coli type iv pilin protein. | the bundle-forming pilus (bfp) of enteropathogenic escherichia coli (epec) is an important virulence factor. we examined the role of divergent alleles of bfpa encoding bundlin, the bfp pilin protein, in pilus biogenesis, pilus interactions, and immune responses. we found that the bfp biogenesis machine from an epec strain that expresses one bundlin type is capable of assembling all other bundlin types. furthermore, we found that epec strains expressing divergent bundlin types are capable of form ... | 2007 | 17635860 |
| the possible influence of luxs in the in vivo virulence of rabbit enteropathogenic escherichia coli. | attaching and effacing (a/e) organisms, such as rabbit enteropathogenic escherichia coli (epec), human epec or enterohemorrhagic e. coli (ehec) share attaching and effacing phenotype and lee pathogenicity island responsible for a/e. the present study was undertaken to investigate the impact of the luxs quorum sensing (qs) signaling system in vitro and in vivo pathogenicity of a/e organisms using rabbit epec (repec) strain e22 (o103:h2). analysis of the bioluminescence indicated abolished product ... | 2007 | 17643872 |
| pharmacokinetics of enrofloxacin and flunixin meglumine and interactions between both drugs after intravenous co-administration in healthy and endotoxaemic rabbits. | the purpose of this study was to determine the pharmacokinetics and possible interactions of enrofloxacin (enr) and flunixin meglumine (fm) in healthy rabbits and in rabbits where endotoxaemia had been induced by administering escherichia coli lipopolysaccharide (lps). six male adult new zealand white rabbits were used for the study. in phase i, fm (2.2 mg/kg) and enr (5 mg/kg) were given simultaneously as a bolus intravenous (iv) injection to each healthy rabbit. after a washout period, phase i ... | 2008 | 17644374 |
| expression and purification of the 26 kda periplasmic protein of brucella abortus: a reagent for the diagnosis of bovine brucellosis. | development of a single diagnostic test for brucellosis in animals is the top priority of present-day research in the field. there is currently a battery of serological tests relying mainly on the use of lps (lipopolysaccharide) as an antigen, culminating in false positives due to serological cross-reactivity. other problems include difficulties in antigen production and the associated biohazard risk. this has prompted the need to develop an alternative antigen to replace lps. in the present stu ... | 2008 | 17685896 |
| translationally controlled tumor protein of brugia malayi functions as an antioxidant protein. | translationally controlled tumor protein (tctp) is one of the most abundantly expressed proteins in the filarial parasites as well as in the other organisms. several functions have been suggested for tctp family of proteins ranging from calcium binding to histamine release function. however, its physiological function is still a mystery. previous studies showed that the expression of tctp is increased several-fold during oxidative stress. in the present work, we report the putative antioxidant f ... | 2007 | 17687568 |
| host protein binding and adhesive properties of h6 and h7 flagella of attaching and effacing escherichia coli. | it had been suggested that the flagella of enteropathogenic escherichia coli (epec) and enterohemorrhagic e. coli (ehec) might contribute to host colonization. in this study, we set out to investigate the adhesive properties of h7 and h6 flagella. we studied the abilities of ehec edl933 (o157:h7) and epec e2348/69 (o127:h6) flagella to bind to bovine mucus, host proteins such as mucins, and extracellular matrix proteins. through several approaches, we found that h6 and h7 flagella and their flag ... | 2007 | 17693516 |
| pet imaging of infection with a hynic-conjugated ltb4 antagonist labeled with f-18 via hydrazone formation. | it was previously shown that the (99m)tc-labeled hydrazinonicotinamide (hynic)-conjugated ltb4 antagonist mb81 visualized infectious foci in rabbits adequately and within a few hours after injection. here, the bivalent hynic-conjugated ltb4 antagonist mb67 (analog of mb81) was fluorinated with (18)f via hydrazone formation and tested in vivo. | 2007 | 17707809 |
| lysophospholipase from the human blood fluke, schistosoma japonicum. | given the unusual nature of the schistosome surface (a highly unusual lipid bi-layer) and the central role of the schistosome tegument in host-parasite relations, an enhanced understanding of the lipid biochemistry of the schistosome surface can be expected to provide new insights into schistosome pathogenesis and lead to new interventions. | 2008 | 17709268 |
| [production and characterization of a polyclonal antibody against rabies virus phosphoprotein]. | the expression of recombinant viral proteins has been a useful tool to study molecular biology and pathogenesis of virus infections. because commercial specific antibodies to rabies virus phosphoprotein (p) are currently unavailable, these antibodies must be generated de novo in order to study the role of p protein during the infectious process. | 2007 | 17713636 |
| the mycobacterium bovis bcg cyclic amp receptor-like protein is a functional dna binding protein in vitro and in vivo, but its activity differs from that of its m. tuberculosis ortholog, rv3676. | mycobacterium tuberculosis rv3676 encodes a cyclic amp (camp) receptor-like protein (crp(mt)) that has been implicated in global gene regulation and may play an important role during tuberculosis infection. the crp(mt) ortholog in mycobacterium bovis bcg, crp(bcg), is dysfunctional in an escherichia coli crp competition assay and has been proposed as a potential source of m. bovis bcg's attenuation. we compared crp(bcg) and crp(mt) in vitro and in vivo, in m. bovis bcg and m. tuberculosis, to ev ... | 2007 | 17785469 |
| spectroscopic validation of the pentameric structure of phospholamban. | phospholamban (pln) regulates calcium translocation within cardiac myocytes by shifting sarco(endo)plasmic reticulum ca(2+)-atpase (serca) affinity for calcium. although the monomeric form of pln (6 kda) is the principal inhibitory species, recent evidence suggests that the pln pentamer (30 kda) also is able to bind serca. to date, several membrane architectures of the pentamer have been proposed, with different topological orientations for the cytoplasmic domain: (i) extended from the bilayer n ... | 2007 | 17804809 |
| [prokaryotic expression of f10 and preparation of its polyclonal antibody]. | to induce the expression of f10 in e.coli and prepare the rabbit polyclonal antibody against it. | 2007 | 17825236 |
| [preparation and characterization of rabbit antibody against human mast cell carboxypeptidase]. | to prepare antibody against human mast cell carboxypeptidase (hmc-cp) by using recombinant hmc-cp expressed in e.coli, and to characterize the antibody. | 2007 | 17825237 |
| efficiency of cell-free protein synthesis based on a crude cell extract from escherichia coli, wheat germ, and rabbit reticulocytes. | the efficiency of protein synthesis for glyceraldehyde-3-phosphate dehydrogenase (g3pdh) was examined with several in vitro coupled transcription/translation protein synthesis systems based on escherichia coli lysate, wheat germ, or reticulocyte lysate, and an in vitro translation system based on wheat germ extract. a significant amount of protein synthesis was observed only in systems based on e. coli using pet/g3pdh as the expression vector. a remarkable increase of protein synthesis was obtai ... | 2008 | 17826860 |
| characterization and expression analysis of tnfr-associated factor 1 (traf1) in grass carp ctenopharyngodon idella. | tnf receptor associated factor 1 (traf1) plays an important role in regulating the tnf signaling and protecting cells from apoptosis. in the present study, a traf1 gene has been cloned from grass carp (ctenopharyngodon idella) by reverse transcription (rt)-pcr and rapid amplification of cdna ends (race). the full-length cdna is 2235bp, including a 250bp 5' utr (untranslated region), a 1659bp open reading frame, and a 326bp 3' utr. the polyadenylation signal (aataaa, aataa) and one mrna instabili ... | 2008 | 17868904 |
| structural and functional characterization of galactooligosaccharides in nostoc commune: beta-d-galactofuranosyl-(1-->6)-[beta-d-galactofuranosyl-(1-->6)]2-beta-d-1,4-anhydrogalactitol and beta-(1-->6)-galactofuranosylated homologues. | a new class of galactooligosaccharides has been identified from the terrestrial cyanobacterium nostoc commune by ms and nmr techniques. these consist of beta-d-galactofuranosyl-(1-->6)-[beta-d-galactofuranosyl-(1-->6)]n-beta-d-1,4-anhydrogalactitols with n ranging from 2 to 8, corresponding to compounds designated 1 through 7. in total these saccharides amounted to approximately 0.35% of the dry thallus of n. commune, while in several other cyanobacteria they were not detected. possibly they pla ... | 2007 | 17935705 |
| protein kinases, tnf-{alpha}, and proteasome contribute in the inhibition of fructose intestinal transport by sepsis in vivo. | lipopolysaccharide (lps) endotoxin is a causative agent of sepsis. the aim of this study was to examine lps effects on intestinal fructose absorption and to decipher mechanisms. sepsis was induced by intravenous injection of lps in rabbits. the ultrastructural study and dna fragmentation patterns were identical in the intestine of lps and sham animals. lps treatment reduced fructose absorption altering both mucosal-to-serosal transepithelial fluxes and uptake into brush border membrane vesicles ... | 2008 | 17962360 |
| [preparation and characterization of the epitopes of sars coronavirus spike protein]. | to prepare the recombinant epitopes of sars-cov spike protein and study their antigenic property to spike protein. | 2007 | 17988578 |
| [prokaryotic expression of human mast cell chymase gene and preparation of its polyclonal antibody]. | to express the human mast cell chymase cdna in e.coli and prepare the antibody against human mast cell chymase with recombinant chymase. | 2007 | 17988583 |
| molecular identification and expression analysis of tumor necrosis factor receptor-associated factor 2 in grass carp ctenopharyngodon idella. | tumor necrosis factor receptor-associated factor 2 (traf2) is a crucial component of almost the entire tumor necrosis factor receptor superfamily signaling pathway. in the present study, a traf2 gene has been cloned from grass carp (ctenopharyngodon idella) by reverse transcription-polymerase chain reaction and rapid amplification of cdna ends. the full-length cdna is 3162 bp, including a 60 bp 5' untranslated region (utr), a 1611 bp open reading frame, and a 1491 bp 3' utr. the polyadenylation ... | 2007 | 17989877 |
| protection against shiga toxin-producing escherichia coli infection by transcutaneous immunization with shiga toxin subunit b. | enterohemorrhagic escherichia coli (ehec) strains are important human food-borne pathogens. ehec strains elaborate potent shiga toxins (stx1, and/or stx2) implicated in the development of hemorrhagic colitis (hc) or hemolytic-uremic syndrome (hus). in this report, we evaluated the immunogenicity and protective efficacy of stx1 subunit b (stxb1) administered by transcutaneous immunization (tci). three groups of dutch belted rabbits received patches containing stxb1, stxb1 in combination with esch ... | 2008 | 18003816 |
| construction and preclinical evaluation of recombinant peru-15 expressing high levels of the cholera toxin b subunit as a vaccine against enterotoxigenic escherichia coli. | enterotoxigenic escherichia coli (etec) is the leading cause of traveler's diarrhea. the heat-labile (lt) and heat-stable (st) toxins mediate etec induced diarrhea. etec strains may express lt, st, or both lt and st, with lt-expressing strains accounting for approximately 50-60% of etec-related traveler's diarrhea. cholera toxin (ct) is >80% homologous to lt and vaccination with ct-b subunit (ct-b) -based vaccines elicit a protective immune response against lt-producing etec strains. peru-15 is ... | 2007 | 18045752 |
| evidence of tandem repeat and extra thiol-groups resulted in the polymeric formation of bovine haptoglobin: a unique structure of hp 2-2 phenotype. | human plasma hp is classified as 1-1, 2-1, and 2-2. they are inherited from two alleles hp 1 and hp 2, but there is only hp 1 in almost all the animal species. hp 2-2 molecule is extremely large and heterogeneous associated with the development of inflammatory-related diseases. in this study, we expressed entire bovine hp in e. coli as a alphabeta linear form. interestingly, the antibodies prepared against this form could recognize the subunit of native hp. in stead of a complicated column metho ... | 2007 | 18047801 |
| quantitative analysis of phosphinothricin-n-acetyltransferase in genetically modified herbicide tolerant pepper by an enzyme-linked immunosorbent assay. | an immunoassay method was developed to quantitatively detect phosphinothricin-n-acetyltransferase (pat) encoded by the bialaphos resistance (bar) gene in genetically modified (gm) pepper. the histidine-tagged pat was overexpressed in escherichia coli m15 (pqe31-bar) and efficiently purified by ni2+ affinity chromatography. a developed sandwich enzyme-linked immunosorbent assay (s-elisa) method (detection limit: 0.01 microg/ml) was 100-fold more sensitive than a competitive indirect elisa (ci-eli ... | 2007 | 18051284 |
| [purification of l-sorbose/l-sorbosne dehydrogenase from ketogulonigenium vulgare and construction and selection of genomic library]. | l-sorbose/l-sorbosone dehydrogenase from ketogulonigenium vulgare s2 can transform l-sorbose to 2-klg, which is widely used in production of vitamin c. in order to obtain the engineering strain producing l-sorbose/l-sorbosone dehydrogenase and simplify the fermentation technology, firstly, this enzyme was purified by the methods of ammonium sulfate precipitation, deae sepharose fast flow and q sepharose high performance. then, the purified l-sorbose/l-sorbosone dehydrogenase was injected to rabb ... | 2007 | 18051871 |
| [cloning and expression of f18 fimbrial operon gene clusters from enterotoxigenic escherichia coli and their bioactivity]. | the fed operon gene clusters with each size of 5.6kb, encoding the f18ab or f18ac fimbriae, was amplified respectively by high fidelity pcr using the genomic dna templates from f18 fimbriae e. coli strains 107/86 or 2134p. the pcr products with the restriction enzyme sites at each end were digested and then cloned into the vector pet-22b (+), the recombinant plamids with the inserts of both type of fed gene clusters were constructed and screened, further confirmed by the means of combination wit ... | 2007 | 18062250 |
| espfu, a type iii-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by e. coli o157:h7. | enterohaemorrhagic escherichia coli (ehec) o157:h7 induces filamentous actin-rich 'pedestals' on intestinal epithelial cells. pedestal formation in vitro requires translocation of bacterial effectors into the host cell, including tir, an ehec receptor, and espf(u), which increases the efficiency of actin assembly initiated by tir. while inactivation of espf(u) does not alter colonization in two reservoir hosts, we utilized two disease models to explore the significance of espf(u)-promoted actin ... | 2008 | 18067584 |
| expression and purification of cysteine introduced recombinant saporin. | saporin, a ribosome inactivating protein is widely used for immunotoxin construction. here we describe a mutation of saporin (sap)-3 dna by introducing a cysteine residue, followed by protein expression and purification by ion exchange chromatography. the purified cys255sap-3, sap-3 isomer and commercially purchased saporin, were tested for toxicity using assays measuring inhibition for protein synthesis. the ic(50) values showed that the toxicity of the cys255sap-3 is equivalent to the sap-3 is ... | 2008 | 18164211 |
| a monoclonal-antibody-based elisa for the detection of human fadd (fas-associated death domain). | fadd (fas-associated death domain) has been widely expressed in various tissues and its expression has been recently demonstrated to correlate with tumour progression and prognosis. currently, measurement of fadd expression mainly depends on western-blot or immunohistochemical approaches. to develop a conventional sandwich elisa avenue for the detection of fadd protein to supplement western blotting or immunohistochemistry, a series of mabs (monoclonal antibodies) specific for fadd protein, desi ... | 2008 | 18167119 |
| biological properties of the native and synthetic lipid a of porphyromonas gingivalis lipopolysaccharide. | a pentaacyl and diphosphoryl lipid a molecule found in the lipid a isolated from porphyromonas gingivalis lipopolysaccharide (lps) was chemically synthesized, and its characteristics were evaluated to reconfirm its interesting bioactivities including low endotoxicity and activity against lps-unresponsive c3h/hej mouse cells. | 2008 | 18173800 |
| expression and purification of human urodilatin by small ubiquitin-related modifier fusion in escherichia coli. | to prevent in vivo degradation, small peptides are usually expressed in fusion proteins from which target peptides can be released by proteolytic or chemical reagents. in this report, small ubiquitin-related modifier (sumo) linked with a hexa-histidine tag was used as a fusion partner for the production of recombinant human urodilatin, a hormone for the treatment of acute decompensated heart failure. the fusion protein, which was overexpressed mainly as inclusion bodies in escherichia coli, cons ... | 2008 | 18189135 |
| beta-synuclein protein from xenopus laevis: overexpression in escherichia coli of the gst-tagged protein and production of polyclonal antibodies. | this report presents a procedure to obtain and purify recombinant beta-synuclein from xenopus laevis expressed in escherichia coli as a glutathione-s-transferase fusion protein. after identification by mass spectrometry, the protein was then used to raise anti-x. laevis beta-synuclein polyclonal antibodies, which were suitable to detect the presence of beta-synuclein in x. laevis brain by western blot. this is the first report of a positive identification of beta-synuclein in an amphibian at the ... | 2007 | 18205611 |
| development of a rapid and sensitive latex agglutination-based method for detection of group a rotavirus. | considering the background of morbidity and mortality caused by human rotavirus, detection methods that use rotavirus group antigen (vp6) in either enzyme immunoassay (eia) or latex agglutination test (lat) has been employed routinely in clinical diagnostic and epidemiological studies. in order to develop a rapid and sensitive rotavirus group a lat, part of segment 6 corresponding to conserved n-terminal portion of the vp6 (1-245 aa) was cloned in escherichia coli expression pgex vector (glutath ... | 2008 | 18241934 |
| preclinical safety evaluation of ifnalpha2a-ngr. | ifnalpha2a-ngr is an antitumor agent of bacterial origin. the report presents the preclinical toxicity studies with ifnalpha2a-ngr in mice, rats and monkeys. the single-dose toxicity study showed no effect on general signs, body weight, food consumption, ophthalmology, hematology and clinical chemistry and necropsy analysis. in repeated-dose toxicity studies, increase in hb was noted both in rat and monkey, showed that ifnalpha2a-ngr may not cause the suppression of hematopoiesis. decrease in as ... | 2008 | 18316145 |
| [prokaryotic expression, purification, and polyclonal antibody generation of beta subunit of human f1f0-atp synthase]. | to express and purify the beta subunit of human f1f0-atp synthase (hatp5b) in prokaryotic system, and generate its polyclonal antibody in rabbits. | 2008 | 18328186 |
| photophysical properties of popular fluorescent adenosine nucleotide analogs used in enzyme mechanism probing. | fluorescent nucleotide analogs are widely used in mechanistic studies of nucleotide binding and utilizing proteins. we describe here an overview of the photophysical parameters of the most popular nucleotide analogs that have a fluorescent n-methylanthraniloyl-group attached at various positions of the nucleotide. steady state absorption and fluorescence spectra of free chromophores depend on the type of modification (ribose, base or phosphate moiety) and the addition of proteins suggests that t ... | 2008 | 18342617 |
| expression and immunogenic comparison of vp2 and vp3 from marine birnavirus. | the coding regions for the major epitopes of structural protein vp2 (vp2e) and structural protein vp3 were amplified from marine birnavirus (mabv) cdna and efficiently expressed as glutathione s-transferase (gst) fusion proteins in escherichia coli. polyclonal antibodies against vp2e and vp3 were raised in rabbits and fish using the purified proteins of gst/vp2e and gst/vp3. the rabbit anti-serum against vp3 was more sensitive than the rabbit anti-vp2e serum in detecting virus in mabv-infected f ... | 2008 | 18353021 |
| the moraxella igd-binding protein mid/hag is an oligomeric autotransporter. | the immunoglobulin d (igd)-binding protein mid/hag of the human respiratory pathogen moraxella catarrhalis is an outer membrane protein of approximately 200kda belonging to the autotransporter family. mid also functions as an adhesin and hemagglutinin. in the present paper, the ultrastructure of mid was mapped. using a series of escherichia coli transformants, the last 210 aa of the c-terminal region were shown to translocate protein mid through the outer membrane suggesting that mid has a beta- ... | 2008 | 18400547 |
| new adhesin of enteroaggregative escherichia coli related to the afa/dr/aaf family. | enteroaggregative escherichia coli (eaec) is an important cause of diarrhea worldwide. we analyzed 17 danish eaec strains, isolated in the course of a case control study, for phenotypic and genotypic properties. the strains belonged to at least 14 different serotypes. using pcr to investigate the prevalence of various putative virulence genes, we found that all but two strains were typical eaec, as they harbored all or part of the previously described aggr regulon. the majority of the strains ha ... | 2008 | 18443096 |
| effect of toll-like receptor 4 blockade on pulmonary inflammation caused by mechanical ventilation and bacterial endotoxin. | mechanical ventilation (mv) and lipopolysaccharide (lps) synergistically increase inflammation and lung injury. the goal of this study was to determine whether blockade of cd14 or toll-like receptor 4 (tlr4) would reduce inflammation caused by lps and mv. rabbits were pretreated with anti-tlr4 or anti-cd14 monoclonal antibodies, followed by endobronchial lps and mv. blockade of tlr4 reduced the number of neutrophils and the amount of cxcl8 in bronchoalveolar lavage fluid. in contrast, blockade o ... | 2008 | 18465402 |
| transient shielding of intimin and the type iii secretion system of enterohemorrhagic and enteropathogenic escherichia coli by a group 4 capsule. | enterohemorrhagic and enteropathogenic escherichia coli (ehec and epec, respectively) strains represent a major global health problem. their virulence is mediated by the concerted activity of an array of virulence factors including toxins, a type iii protein secretion system (ttss), pili, and others. we previously showed that epec o127 forms a group 4 capsule (g4c), and in this report we show that ehec o157 also produces a g4c, whose assembly is dependent on the etp, etk, and wzy genes. we furth ... | 2008 | 18502854 |
| espf interacts with nucleation-promoting factors to recruit junctional proteins into pedestals for pedestal maturation and disruption of paracellular permeability. | many pathogenic bacteria subvert normal host cell processes by delivering effector proteins which mimic eukaryotic functions directly into target cells. espf is a multifunctional protein injected into host cells by attaching and effacing pathogens, but its mechanism of action is not understood completely. in silico analyses of espf revealed two key motifs: proline-rich domains and pdz domain binding motifs. such functional domains may allow espf to act as an actin nucleation-promoting factor by ... | 2008 | 18559425 |
| [expression of human hzf1 in e. coli and preparation of antibody against human hzf1 protein]. | to express human hzf1 fusion protein in e. coli and to obtain an anti-hzf1 antibody. | 2007 | 18595257 |
| prokaryotic expression of truncated vp7 of bluetongue virus (btv) and reactivity of the purified recombinant protein with all btv type-specific sera. | purification of bluetongue virus (btv) group-specific vp7 protein, expressed in prokaryotic system as histidine-tagged fusion protein is described in the present study. the major antigenic portion of vp7 gene of btv 23 was amplified from the extracted rna by reverse transcription polymerase chain reaction and cloned. the recombinant expression construct (pet-vp7) was identified by the polymerase chain reaction and sequencing analysis. expression of histidine-tagged fusion truncated vp7 protein w ... | 2008 | 18611412 |
| plasmodium falciparum: enhanced soluble expression, purification and biochemical characterization of lactate dehydrogenase. | plasmodium lactate dehydrogenase (pldh), owing to unique structural and kinetic properties, is a well known target for antimalarial compounds. to explore a new approach for high level soluble expression of plasmodium falciparum lactate dehydrogenase (pfldh) in e. coli, pfldh encoding sequence was cloned into pqe-30 xa vector. when transformed e. coli sg13009 cells were induced at 37 degrees c with 0.5mm isopropyl beta-d-thiogalactoside (iptg) concentration, the protein was found to be exclusivel ... | 2008 | 18619439 |
| a recombinant antigen-based elisa for the simultaneous differential serodiagnosis of mycoplasma gallisepticum, mycoplasma synoviae, and mycoplasma meleagridis infections. | we have previously identified species-specific dna fragments, referred to as ms2/28 and mm14, of mycoplasma synoviae and mycoplasma meleagridis, respectively. in the present study, we extended our analysis of the ms2/28 fragment that was found to encode a species-specific antigenic site, and we demonstrated the specificity of the mycoplasma gallisepticum hemagglutinin protein encoded by pmga1.2 (a member of the vlha gene family). then, we combined the escherichia coli-expressed products of ms2/2 ... | 2008 | 18646449 |
| a comparative study of protein synthesis in in vitro systems: from the prokaryotic reconstituted to the eukaryotic extract-based. | cell-free protein synthesis is not only a rapid and high throughput technology to obtain proteins from their genes, but also provides an in vitro platform to study protein translation and folding. a detailed comparison of in vitro protein synthesis in different cell-free systems may provide insights to their biological differences and guidelines for their applications. | 2008 | 18664286 |
| [gapdh activity and immunogenicity of staphylococcus aureus recombinant gapc protein]. | in order to characterize the glyceraldehyde-3-phosphate dehydrogenase (gapdh) activity, immunogenicity and immunoprotection of the staphylococcus aureus (s. aureus) surface protein gapc, gapc gene of s. aureus was amplified from strain bmsa/855/23-1 by pcr, and was inserted into pqe-30 vector subsequently. the recombinant plasmid, designated as pqe/gapc, was transformed into e. coli strain m15 (prep4). the recombinant gapc fusion proein was successfully expressed in e. coli m15 induced with iptg ... | 2008 | 18724693 |
| infection of rabbit kidney cells (rk13) by enteropathogenic escherichia coli as a model to study the dynamics of actin cytoskeleton. | enteropathogenic escherichia coli (epec) colonizes the intestinal mucosa and causes a cell lesion known as attachment and effacement (a/e) lesion. the molecular mechanisms for a/e lesions include injection of tir, which is a receptor for an adhesin named intimin. the tir-intimin interaction causes rearrangement of the cytoskeleton forming actin-rich structures called pedestals. unfortunately, the formation of the a/e lesions and the dynamics of the actin cytoskeleton during this rearrangement in ... | 2008 | 18772938 |
| antioxidant, antihypertensive, and antibacterial properties of endophytic pestalotiopsis species from medicinal plants. | pestalotiopsis species were most dominant endophytic species isolated from four medicinal plants including terminalia arjuna, terminalia chebula, azadirachta indica, and holarrhena antidysenterica. thirty pestalotiopsis species isolated from different parts of the medicinal plants were selected for the study. the antioxidant and antihypertensive properties of pestalotiopsis isolates were determined by measuring 1,1-diphenyl-2-picrylhydrazyl inhibitory activity, lipid peroxidation, and angiotensi ... | 2008 | 18772940 |
| immunogenicity of a chicken viral newcastle disease virus f gene-c3d fusion protein containing a chicken homologue of c3d. | the gene fragment coding for arbor acres (aa) chicken c3d gene (chc3d) was cloned and expressed as a component of fusion proteins for its potential use as a vaccine for chickens and for in vitro experiments. this fragment of complement protein c3 (c3d) has been shown to strengthen b-cell responses when complexed with antigen. three potential vaccine constructs were engineered to contain two, four, and six copies of chc3d-p29 coding gene, which was linked to the f gene of newcastle disease virus ... | 2008 | 18788947 |
| role of the histone-like nucleoid structuring protein in the regulation of rpos and rpos-dependent genes in vibrio cholerae. | production of the zn-metalloprotease hemagglutinin (ha)/protease by vibrio cholerae has been reported to enhance enterotoxicity in rabbit ileal loops and the reactogenicity of live cholera vaccine candidates. expression of ha/protease requires the alternate sigma factor sigma(s) (rpos), encoded by rpos. the histone-like nucleoid structuring protein (h-ns) has been shown to repress rpos expression in escherichia coli. in v. cholerae strains of the classical biotype, h-ns has been reported to sile ... | 2008 | 18790865 |
| cloning of a gene fragment encoding chicken complement component c3d with expression and immunogenicity of newcastle disease virus f gene-c3d fusion protein. | the gene fragment coding for the complement protein c3 (chc3d) from arbor acres (aa) chickens was cloned and expressed as a fusion protein for its application in the vaccine study of chicken, and for in vitro experiments. the chc3d fragment strengthened b-cell responses when complexed with antigen. three potential vaccine construct units were engineered to contain two, four and six copies of the chc3d-p29 coding gene linked to the f gene of newcastle disease virus, an economically important path ... | 2008 | 18798021 |
| expression of pisum sativum sad polypeptides in production hosts and in planta: tetrameric organization of the protein. | in pisum sativum, the short-chain alcohol dehydrogenase-like protein (sad) gene family consists of at least three members (sad-a, -b, and -c). expression of two of these genes (sad-a and -c) in escherichia coli or pichia pastoris resulted in full-length soluble proteins. purified sad-a was used as antigen for antibody production in rabbits. with these antibodies the recombinant sad-c protein (which was most highly expressed of the two isoforms) was shown to be a tetramer consisting of a dimer of ... | 2009 | 18814850 |
| determination of the mimic epitope of the m-like protein adhesin in swine streptococcus equi subsp. zooepidemicus. | the m-like protein, also known as szp, is expressed on the surface of streptococcus equi subsp. zooepidemicus (s. zooepidemicus). previous studies demonstrated that szp is similar to m protein of group a streptococcus in the structure and characteristics of antiphagocytosis. the m protein is an adhesin that can bind to the host cells, however it is not known whether the szp of s. zooepidemicus also functions as an adhesin. we conducted an investigation to determine szp as an adhesin, and one szp ... | 2008 | 18840263 |
| a study of the relationship between the fever caused by bacterial pyrogens and the fever accompanying acute infections. | the relationship between the fever of acute infection and that following injection of bacterial pyrogen was studied by administering pyrogens to animals convalescent from acute infections. rabbits surviving dermal pneumococcal infections or peritonitis due to escherichia coli were given intravenous injections of typhoid or e. coli vaccine. they showed no evidence of tolerance to the fever-promoting effect of these pyrogenic materials. tolerance did develop in infected animals given daily pyrogen ... | 1948 | 18881485 |
| immunization with recombinant actin from trypanosoma evansi induces protective immunity against t. evansi, t. equiperdum and t. b. brucei infection. | actin gene of trypanosoma evansi (stib 806) was cloned and expressed in escherichia coli. the predicted amino acid sequence of t. evansi actin shows 100%, 98.7%, and 93.1%, homology with trypanosoma equiperdum, trypanosoma brucei brucei, and trypanosoma cruzi. recombinant actin was expressed as inclusion bodies in e. coli. it was purified and renatured for immunological studies. mice immunized with the renatured recombinant actin were protected from lethal challenge with t. evansi stib 806, t. e ... | 2009 | 18923843 |
| [a comparative study on the properties of trichosanthin before and after site-directed pegylation]. | to construct pegylated trichosanthin (tcs) mutein and analyze its bioactivities, immunogenicity, acute toxicity, and pharmacokinetics. | 2008 | 18953888 |
| review. the mammalian proton-coupled peptide cotransporter pept1: sitting on the transporter-channel fence? | the proton-coupled di- and tripeptide transporter pept1 (slc15a1) is the major route by which dietary nitrogen is taken up from the small intestine, as well as being the route of entry for important therapeutic (pro)drugs such as the beta-lactam antibiotics, angiotensin-converting enzyme inhibitors and antiviral and anti-cancer agents. pept1 is a member of the major facilitator superfamily of 12 transmembrane domain transporter proteins. expression studies in xenopus laevis on rabbit pept1 that ... | 2009 | 18957377 |
| [recombinant envelope glycoprotein domain iii of dengue virus inhibit virus infection]. | to observe the ability of dengue virus recombinant envelope protein domain expressed in e. coli to inhibit virus infection and induce the neutralizing antibody. | 2008 | 19031695 |
| production of monospecific rabbit antisera recognizing nidovirus proteins. | the importance of monospecific antisera for the experimental analysis of viral proteins is undisputed. they make it possible to identify and analyze the target protein against a background of a large number of other proteins, either in whole fixed cells or in cell lysates. this chapter describes our experience with the production of such rabbit antisera directed against proteins of coronaviruses and other nidoviruses. the use as antigens of either synthetic peptides (coupled to a carrier protein ... | 2008 | 19057875 |
| functional analysis of the disulphide loop mutant of staphylococcal enterotoxin c2. | the superantigen staphylococcal enterotoxin c2 (sec2) tremendously activate t lymphocytes bearing certain t-cell receptor vbeta domains when binding to mhc ii molecules, which launches a powerful response of tumour inhibition in vitro as well as in vivo. however, the toxicity of sec2 performed in clinic limited its broad application for immunotherapy. the previous studies suggested that the disulphide loop may be important for the toxicity of some ses, which prompted us to investigate the potent ... | 2009 | 19082587 |
| [prokaryotic expression of trigeminy artificial fusion gene of leptospira interrogans and the immunogenicity of its products]. | to construct lipl32/1-lipl21-ompl1/2 fusion gene of leptospira interrogans and its prokaryotic expression system, and to identify the immunogenicity of its products. | 2008 | 19084958 |
| production of polyclonal antibodies to a recombinant coat protein of potato virus y. | the gene encoding the coat protein (cp) of a potato virus y (pvy) was cloned into expression vector pmpm-a4omega. pvy cp was expressed in escherichia coli and the purified recombinant protein was used for raising rabbit polyclonal antibodies. the sera and antibodies were tested for the detection of pvy in the laboratory host nicotiana tabacum cv. petit havana sr1 and in various cultivars of the natural host solanum tuberosum by elisa as well as by western blots. the antibodies can be used for th ... | 2008 | 19085080 |
| use of a bacterially expressed human factor ix light chain to develop polyclonal antibody anti-hfix. | hemophilia b is an x-linked recessive bleeding disorder caused by deficiency or malfunctioning of human coagulation factor ix (hfix). hemophilia b patients are treated at present by infusion of plasma derived hfix which is not always efficient, because development of anti-hfix antibodies (alloantibodies) in some cases inhibits the activity of the infused hfix. the hfix alloantibodies are directed against gamma-carboxyglutamic acid residues (gla-domain) or protease domain in hfix light chain. an ... | 2009 | 19145409 |