| in vitro potency of various polymyxin b components. | | 2011 | 21709096 |
| detection of volatile compounds produced by microbial growth in urine by selected ion flow tube mass spectrometry (sift-ms). | selected ion flow tube-mass spectrometry has been used to measure the volatile compounds occurring in the headspace of urine samples inoculated with common urinary tract infection (uti)-causing microbes escherichia coli, proteus vulgaris, pseudomonas aeruginosa, staphylococcus aureus, staphylococcus epidermidis, klebsiella pneumoniae, enterococcus faecalis, or candida albicans. this technique has the potential to offer rapid and simple diagnosis of the causative agent of utis. | 2011 | 21741416 |
| arthritis, osteomyelitis, septicemia and meningitis caused by klebsiella in a low-birth-weight newborn: a case report. | abstract: | 2011 | 21707974 |
| investigation of the antimicrobial activity of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.). | the aim of the study was to determine antimicrobial activity of rhaponticum and shrubby cinquefoil extracts. material and methods. ethanol extract from the leaves of rhaponticum (rhaponticum carthamoides d.c. iljin) and shrubby cinquefoil (potentilla fruticosa l.) was produced at the department of food technology, kaunas university of technology. the antimicrobial activity of the viscous extract or rhaponticum and shrubby cinquefoil was evaluated using standard microorganism cultures (bacteria s ... | 2011 | 21822040 |
| novel variants of the qnrb gene, qnrb31 and qnrb32, in klebsiella pneumoniae. | quinolone resistance in the enterobacteriaceae families is mostly attributed to the accumulation of mutations in the bacterial enzymes targeted by fluoroquinolones: dna gyrase and dna topoisomerase iv. here we isolated the strains kp3606 and kp4707 in klebsiella pneumoniae in different specimens from 2008 to 2010 in taizhou municipal hospital of china, discovered a new subtype qnrb31, which genbank accession number is hq418999, and another new subtype qnrb32, which genbank accession number is hq ... | 2011 | 21816942 |
| does it ever make sense to treat a bacterial infection with a drug against which the responsible pathogen possesses a resistance mechanism? | | 2011 | 21689208 |
| Identification and Characterization of OXA-48 Producing, Carbapenem-Resistant Enterobacteriaceae Isolates in Turkey. | Carbapenem resistance in Enterobacteriaceae isolates has been reported from Turkey and is most often mediated by OXA-48 type carbapenemases. We report the identification and characterization of four carbapenem-resistant isolates (three Klebsiella pneumoniae and one Escherichia coli) among 515 clinical Enterobacteriaceae isolates collected during a 7-month study period in Ankara, Turkey. The four isolates were recovered from blood and urine specimens in patients with varied clinical manifestation ... | 2011 | 21844575 |
| outcome of carbapenem resistant klebsiella pneumoniae bloodstream infections. | the aim of this study was to evaluate the impact of carbapenem-resistant k. pneumoniae bloodstream infections on mortality. during the study period 42, 68 and 120 patients were identified with carbapenem-resistant, extended-spectrum β-lactamase producers (esbl) and susceptible k. pneumoniae bloodstream infections, respectively. patients with carbapenem-resistant k. pneumoniae had higher rates of prior antimicrobial exposure, other nosocomial infections, and use of invasive devices. infection-rel ... | 2011 | 21722257 |
| untreated primary thyroid lymphoma in an elderly woman. | | 2011 | 21727773 |
| [microbiology of surgical site infections in abdominal tract surgery patients]. | knowledge of the microbiology of surgical infections after abdominal surgery can be of use when prescribing effective empirical antibiotic treatments. | 2011 | 21820107 |
| antisense inhibition of gene expression and growth in gram-negative bacteria by cell-penetrating peptide conjugates of peptide nucleic acids targeted to rpod gene. | gram-negative bacteria (gnb) cause common and severe hospital- and community-acquired infections with a high incidence of multidrug resistance (mdr) and mortality. the emergence and spread of mdr-gnb strains limit therapeutic options and highlight the need to develop new therapeutic strategies. in this study, the peptide (rxr)(4)xb- and (kff)(3)k-conjugated peptide nucleic acids (ppnas) were developed to target rpod, which encodes an rna polymerase primary σ(70) that is thought to be essential f ... | 2011 | 22000398 |
| enhanced reducing equivalent generation for 1,3-propanediol production through cofermentation of glycerol and xylose by klebsiella pneumoniae. | 1,3-propanediol (1,3-pd) biosynthesis plays a key role in nadh consumption to regulate the intracellular reducing equivalent balance of klebsiella pneumoniae. this study aimed to increase reducing equivalent for enhancing 1,3-pd production through cofermentation of glycerol and xylose. adding xylose as cosubstrate resulted in more reducing equivalent generation and higher cell growth. in batch fermentation under microaerobic condition, the 1,3-pd concentration, conversion from glycerol, and biom ... | 2011 | 21960271 |
| quantification and analysis of airborne bacterial characteristics in a nursing care institution. | indoor air quality has become a critical issue because people spend most of their time in the indoor environment. the factors that influence indoor air quality are very important to environmental sanitation and air quality improvement. this study focuses on monitoring air quality, colony counts, and bacteria species of the indoor air of a nursing care institution. the regular colony counts in two different wards range from 55 to 600 cfu m(-3) regression analysis results indicate that the bacteri ... | 2011 | 21850827 |
| seasonal and temperature-associated increases in gram-negative bacterial bloodstream infections among hospitalized patients. | knowledge of seasonal trends in hospital-associated infection incidence may improve surveillance and help guide the design and evaluation of infection prevention interventions. we estimated seasonal variation in the frequencies of inpatient bloodstream infections (bsis) caused by common bacterial pathogens and examined associations of monthly bsi frequencies with ambient outdoor temperature, precipitation, and humidity levels. | 2011 | 21966489 |
| use of ceramic-based cell immobilization to produce 1,3-propanediol from biodiesel-derived waste glycerol with klebsiella pneumoniae. | the feasibility of the continuous production of a valuable bioplastic raw material, namely 1,3-propanediol (1,3-pdo) from biodiesel by-product glycerol, using immobilized cells was investigated. in addition, the effect of hydraulic retention time (hrt) was also analysed. | 2011 | 21883732 |
| influence of blocking of 2,3-butanediol pathway on glycerol metabolism for 1,3-propanediol production by klebsiella oxytoca. | glycerol metabolism is a typical biological oxidoreductive reaction. 1,3-propanediol (1,3-pd) is the final product of the reductive branch, while acetate, succinate, lactate, 2,3-butanediol (2,3-bd), and ethanol were produced in the oxidative branch. 2,3-bd, which has similar properties of high boiling point and water solubility with 1,3-pd, not only contests the carbon flow and nadh with 1,3-pd but also serves as an obstacle for obtaining high purity 1,3-pd in downstream processes. in this stud ... | 2011 | 21915590 |
| production of (2s,3s)-2,3-butanediol and (3s)-acetoin from glucose using resting cells of klebsiella pneumonia and bacillus subtilis. | production of highly pure (2s,3s)-2,3-butanediol ((2s,3s)-2,3-bd) and (3s)-acetoin ((3s)-ac) in high concentrations is desirable but difficult to achieve. in the present study, glucose was first transformed to a mixture of (2s,3s)-2,3-bd and meso-2,3-bd by resting cells of klebsiella pneumoniae cicc 10011, followed by biocatalytic resolution of the mixture by resting cells of bacillus subtilis 168. meso-2,3-bd was transformed to (3s)-ac, leaving (2s,3s)-2,3-bd in the reaction medium. using this ... | 2011 | 21945208 |
| Prevalence and characterization of extended spectrum ß-lactamases in Klebsiella-Enterobacter-Serratia group bacteria, in Algeria. | OBJECTIVES: The authors had for aim to assess the local epidemiology, antibiotic resistance, and molecular typing of expanded spectrum betalactamase producing Klebsiella, Enterobacter, and Serratia (ESBL KES). MATERIALS AND METHODS: Two hundred and seven strains of the KES group were isolated in the microbiology laboratory of the Annaba Ibn Rochd hospital in 2009. The antibiotic resistance (diffusion method and MIC) was tested and ESBL detection was performed as recommended by the Clinical Labor ... | 2011 | 22056377 |
| partial excision of blakpc from tn4401 in carbapenem-resistant klebsiella pneumoniae. | we describe a novel tn4401 variant (tn4401d) in epidemic klebsiella pneumoniae clone st258, from which a partial bla(kpc) fragment has been excised along with iskpn7 and a partial tnpa fragment. nested pcr experiments confirmed that this region can be removed from distinct tn4401 isoforms in both k. pneumoniae and escherichia coli. this study highlights that the region surrounding bla(kpc) is undergoing recombination, and that tn4401 itself is heterogeneous and highly plastic. | 2011 | 22203593 |
| an effective intervention to limit the spread of an epidemic carbapenem-resistant klebsiella pneumoniae strain in an acute care setting: from theory to practice. | the highly transmissible and virulent carbapenem-resistant klebsiella pneumoniae (crkp) kpc-3 strain has been spreading in our medical center and in other centers in israel since 2006. an intervention that aimed to diminish its prevalence was constructed and applied in our institute. | 2011 | 21864942 |
| reduction of molybdate to molybdenum blue by klebsiella sp. strain hkeem. | a novel molybdate-reducing bacterium, tentatively identified as klebsiella sp. strain hkeem and based on partial 16s rdna gene sequencing and phylogenetic analysis, has been isolated. strain hkeem produced 3 times more molybdenum blue than serratia sp. strain dr.y8; the most potent mo-reducing bacterium isolated to date. molybdate was optimally reduced to molybdenum blue using 4.5 mm phosphate, 80 mm molybdate and using 1% (w/v) fructose as a carbon source. molybdate reduction was optimum at 30 ... | 2011 | 22052341 |
| In vitro analysis of the bactericidal activity of Escherichia coli Nissle 1917 against pediatric uropathogens. | The usefulness of prophylactic antibiotics to prevent recurrent urinary tract infections in children was recently questioned. Some groups have attempted to use probiotics, most commonly lactobacillus, to prevent recurrent infections by altering the intestinal bacterial reservoir with variable results. Mutaflor® is a possible alternative probiotic in which the active agent is Nissle 1917. Nissle 1917 is a commensal Escherichia coli strain that eradicates pathogenic bacteria from the gastrointesti ... | 2011 | 21855931 |
| the antimicrobial efficacy of silver on antibiotic-resistant bacteria isolated from burn wounds. | the antibiotic-resistant bacteria are a major concern to wound care because of their ability to resist many of the antibiotics used today to treat infections. consequently, other antimicrobials, in particular ionic silver, are considered ideal topical agents for effectively helping to manage and prevent local infections. little is known about the antimicrobial efficacy of ionic silver on antibiotic-resistant bacteria at different ph values. consequently, in this study our aim was to evaluate the ... | 2011 | 22182219 |
| plasmid typing and genetic context of ampc β-lactamases in enterobacteriaceae lacking inducible chromosomal ampc genes: findings from a spanish hospital 1999-2007. | to gain insights into ampc transmission between bacterial strains. | 2012 | 21980067 |
| europe launches 12 point plan to tackle antimicrobial resistance. | | 2011 | 22106364 |
| carbapenem-hydrolyzing class d β-lactamase oxa-48 in klebsiella pneumoniae isolates from tunisia. | two carbapenem-resistant klebsiella pneumoniae isolates producing the plasmid-encoded carbapenem-hydrolyzing oxa-48 were identified. these isolates, recovered from two patients hospitalized in two different hospitals in tunisia in december 2010, were not clonally related. molecular investigations showed that both isolates co-produced the narrow-spectrum β-lactamases tem-1 and shv-1, together with the extended-spectrum β-lactamase ctx--15. | 2011 | 21915739 |
| immune restoration klebsiella lymphadenitis. | a subset of hiv-infected patients who receive antiretroviral therapy (art) develop immune restoration syndrome. a case of immune restoration klebsiella lymphadenitis is described. fine needle aspiration cytology remains a useful investigative tool in the post-art era, particularly in the diagnosis of persistent generalized lymphadenopathy, infection and malignancy of the lymph nodes. | 2011 | 21890558 |
| a survey on urinary tract infections associated with the three most common uropathogenic bacteria. | objectives: the most common uropathogenic gram negative bacteria are escherichia coli and klebsiella pneumoniae. the purpose of this study was to determine the three most frequent bacterial agents causing urinary tract infections (uti) in patients who referred to central laboratory of dr. shariati hospital, during 2 years (january 2006- december 2007).materials and methods: the registered data were checked and collected by the questionnaires as a retrospective epidemiological survey. then, the c ... | 2010 | 21977133 |
| Impact of solvent/detergent treatment of plasma on transfusion-relevant bacteria. | Background A solvent/detergent (S/D) treatment in a medical device has been developed for pathogen reduction of plasma for transfusion. Impact of S/D on bacterial growth and on the capacity of complement to kill bacteria has been investigated in this study. Study design and methods A pool of apheresis plasma from four donors was spiked with eight transfusion-relevant bacteria. Plasma was treated with 1% tri(n-butyl) phosphate and 1% Triton X-45 at 31°C for 90 min and then extracted by oil at 3 ... | 2011 | 22092109 |
| characteristics of healthcare-associated and community-acquired klebsiella pneumoniae bacteremia in taiwan. | klebsiella pneumoniae is the major cause of community-onset pyogenic infections in taiwan. we investigated the clinical features and outcomes of community-acquired (ca) and healthcare-associated (hca) infections among community-onset k. pneumoniae bacteremia. | 2011 | 22101080 |
| Bloodstream infections in adult patients with cancer: clinical features and pathogenic significance of Staphylococcus aureus bacteremia. | OBJECTIVES: The aim of this study was to more precisely delineate the characteristics and outcomes of bloodstream infections in adult cancer patients. METHODS: Using a database for nationwide surveillance of bacteremia, we analyzed data related to bacteremia in adult patients with cancer in order to evaluate clinical features and outcomes and to define predictive factors for mortality. ... | 2011 | 22193772 |
| vim and imp metallo-β-lactamases and other extended-spectrum β-lactamases in escherichia coli and klebsiella pneumoniae from environmental samples in a tunisian hospital. | an extremely drug-resistant enterobacteriaceae species emerged in kasserine hospital, tunisia between 2009 and 2010 causing a local outbreak. we aimed to characterize extended-spectrum β-lactamase (esbl) and metallo-β-lactamase (mbl)-producing enterobacteriaceae from the hospital environment. swabs were collected from ten different wards from kasserine hospital, tunisia. a total of 46 isolates were cultured onto macconkey agar supplemented with ceftazidime to select for esbl-producing enterobact ... | 2011 | 21917010 |
| [Aspects of the antimicrobial resistence profile in infections with Escherichia coli and Klebsiella pneumoniae in diabetic patients]. | Infections in diabetic patient remains an important cause of morbidity and mortality, triggering and maintaining a prolonged metabolic imbalance. Emergence of extented spectrum beta-lactmase (ESBL) in Escherichia coli and Klebsiella pneumoniae is a major concern, because of the atypical manner infection acts in this group of imunodepressed patients and also for the limited therapeutic solutions. For this reason we have evaluated the profile of antimicrobial resistance of these pathogens in both ... | 2011 | 22046785 |
| The optimization of biohydrogen production by bacteria using residual glycerol from biodiesel synthesis. | In this research the production of hydrogen by Klebsiella pneumoniae BLb01 using residual glycerol discharged from a biodiesel fuel production plant was investigated. Klebsiella pneumoniae BLb01 was isolated from a bacteria-rich sludge of an upflow anaerobic sludge blanket reactor (UASB) of a soybean processing plant. A Plackett-Burman design (P-B) and Response Surface Methodology (RSM) were employed to determine the optimal condition for enhanced hydrogen production. The maximal hydrogen produc ... | 2011 | 21967246 |
| Bacterial nitrate assimilation: gene distribution and regulation. | In the context of the global nitrogen cycle, the importance of inorganic nitrate for the nutrition and growth of marine and freshwater autotrophic phytoplankton has long been recognized. In contrast, the utilization of nitrate by heterotrophic bacteria has historically received less attention because the primary role of these organisms has classically been considered to be the decomposition and mineralization of dissolved and particulate organic nitrogen. In the pre-genome sequence era, it was k ... | 2011 | 22103536 |
| Clonal dissemination of Klebsiella pneumoniae ST258 harbouring KPC-2 in Argentina. | The present work describes the abrupt emergence of Klebsiella pneumoniae carbapenemase (KPC) and characterizes the first 79 KPC-producing enterobacteria from Argentina (isolated from 2006 to 2010). The emergence of bla(KPC-2) was characterized by two patterns of dispersion: the first was the sporadic occurrence in diverse enterobacteria from distant geographical regions, harbouring plasmids of different incompatibility groups and bla(KPC-2) in an unusual genetic environment flanked by ISKpn8-?bl ... | 2011 | 21851480 |
| Characterization of the inhibitor-resistant SHV ß-lactamase (SHV-107) in a clinical Klebsiella pneumoniae strain co-producing GES-7 enzyme. | The clinical K. pneumoniae INSRA6884 strain exhibited non-susceptibility to all penicillins tested (MICs of 64 to >2048 µg/ml). The MICs of penicillins were weakly reduced by clavulanate (from 2048 to 512 µg/ml), and tazobactam restored piperacillin susceptibility. Molecular characterization identified the genes bla(GES-7) and a new ß-lactamase gene bla(SHV-107), which encoded an enzyme that differed from SHV-1 by the amino acid substitutions Leu35Gln and Thr235Ala. The SHV-107-producing E. coli ... | 2011 | 22083476 |
| Risk factors for bloodstream infections caused by extended-spectrum ß-lactamase-producing Escherichia coli and Klebsiella pneumoniae. | The objective of this study was to identify risk factors for bacteremia by extended-spectrum ß-lactamase (ESBL)-producing Escherichia coli and Klebsiella pneumoniae. Retrospective case-control study performed in a 450-bed acute care academic tertiary hospital in Barcelona, Spain. Cases included 53 patients with ESBL-producing E. coli or K. pneumoniae bacteremia, and 159 controls with non-ESBL-producing E. coli or K. pneumoniae bacteremia. Controls were matched in a 3:1 ratio to case patients acc ... | 2011 | 21861009 |
| Development of an antibiogram in a long-term care facility. | To create an antibiogram-a profile of an organism's susceptibility/resistance to a panel of antibiotics- for a long-term care facility to assess the prevalence of resistance of bacteria present at the facility. | 2011 | 22079792 |
| Antimicrobial susceptibility of Gram-negative organisms from intraabdominal infections and evolution of isolates with extended spectrum ß-lactamases in the SMART study in Spain (2002-2010). | Introduction. The SMART (Study for Monitoring Antimicrobial Resistance Trends) surveillance study records the antimicrobial susceptibility of Gram-negative bacilli obtain from intraabdominal infections with special focus in isolates with extended spectrum ß-lactamases (ESBLs). Material and Methods. The antimicrobial susceptibility of 8,869 isolates was analyzed by microdilution during the SMART study performed in Spain from 2002 to 2010. Isolates were recovered in 16 centres. Results. Escherichi ... | 2011 | 22173194 |
| successful treatment of ndm-1 klebsiella pneumoniae bacteraemia in a neutropenic patient. | the rapid emergence of novel multidrug-resistant organisms coupled with the slow development of new antibiotics is of great concern. with the discovery of new delhi metallo-beta-lactamase 1 (ndm-1)-producing klebsiella pneumoniae, clinicians are faced with problems in the treatment of infections caused by this multidrug-resistant organism. therapeutic experiences are limited. we share our experience of the successful treatment of a febrile neutropenic patient with ndm-1-producing k. pneumoniae b ... | 2011 | 22126430 |
| oral levofloxacin brings better results in treating endogenous klebsiella pneumoniae endophthalmitis. | | 2011 | 22149484 |
| in vivo comparison of cxa-101 (fr264205) with and without tazobactam versus piperacillin-tazobactam using human simulated exposures against phenotypically diverse gram-negative organisms. | cxa-101 is a novel antipseudomonal cephalosporin with enhanced activity against gram-negative organisms displaying various resistance mechanisms. this study evaluates the efficacy of exposures approximating human percent free time above the mic (%ft > mic) of cxa-101 with or without tazobactam and piperacillin-tazobactam (tzp) against target gram-negative organisms, including those expressing extended-spectrum β-lactamases (esbls). sixteen clinical gram-negative isolates (6 pseudomonas aeruginos ... | 2012 | 22064538 |
| effectiveness of antibiotic-lock therapy for long-term catheter-related bacteremia due to gram-negative bacilli: a prospective observational study. | a prospective observational study evaluated the effectiveness of combining antibiotic-lock therapy and systemic antibiotics for gram-negative bacilli long-term catheter-related bacteremia. in 46 uncomplicated episodes, the most frequently isolated microorganisms were pseudomonas aeruginosa (15), enterobacter cloacae (12), escherichia coli (10), and klebsiella spp. (8). cure was achieved in 95% of cases. | 2011 | 21890750 |
| nosocomial infection and characterization of extended-spectrum β-lactamases-producing enterobacteriaceae in northeast brazil. | extended spectrum β-lactamases (esbls) are enzymes that degrade β-lactam antibiotics and have been reported to be an important cause of nosocomial infection in worldwide. | 2011 | 21860889 |
| Inter-laboratory comparison of different rapid methods for the detection of bacterial contamination in platelet concentrates. | Background Bacterial contamination of platelet concentrates still represents a major risk in transfusion medicine, and a variety of screening methods have been available to improve the safety of PCs. In the present study, the analytical quality of three different rapid screening methods (BactiFlow flow cytometry, Pan Genera Detection Assay, 23S rRNA RT-PCR) was evaluated in an inter-laboratory comparison in three different German blood services. Methods Samples were inoculated with different b ... | 2011 | 22150778 |
| Device-associated infection rates in 398 intensive care units in Shanghai, China: International Nosocomial Infection Control Consortium (INICC) findings. | To determine device-associated healthcare-associated infection (DA-HAI) rates and the microorganism profile in 398 intensive care units (ICUs) of 70 hospitals in Shanghai, China. | 2011 | 21846591 |
| Readministration of drotrecogin alfa (activated) in an adult with severe sepsis. | The case of a patient with severe, multidrug-resistant, postoperative sepsis who was successfully treated with drotrecogin alfa (activated) on two occasions is reported. | 2011 | 21880886 |
| [severe congenital neutropenia]. | severe congenital neutropenia is a primary immunodeficiency; the lack of maturation of neutrophil precursor in bone marrow and severe neutropenia are the subjacent characteristics which explain a marked susceptibility to severe and recurrent infections; bacteria and fungi are the most common etiologic agents. we report the case of an infant with severe congenital neutropenia that began at 4 days from birth with perianal abscess infections, sepsis, liver abscess, gingivitis and oral ulcers; she r ... | 2010 | 21854729 |
| Over-treatment of carbapenemase-producing Enterobacteriaceae. | Objectives: To describe the treatment and outcomes of patients with carbapenemase-producing Enterobacteriaceae and evaluate whether these cases represented active infection requiring antibiotic therapy or colonization. Methods: Adult inpatients with carbapenemase-producing Enterobacteriaceae were retrospectively evaluated. Cases were classified as colonization versus infection by 2 infectious diseases physicians. Multiple cultures that grew in the same patient within a 2-week period were evaluat ... | 2011 | 22200121 |
| polymyxin combination therapy and the use of serum bactericidal titers in the management of kpc-producing klebsiella pneumoniae infections: a report of 3 cases. | management of patients with kpc-harboring enterobacteriaceae has become a significant and challenging scenario. we report three cases of kpc-producing klebsiella pneumoniae bacteremia that were successfully treated using combination therapy with polymyxin b and other antimicrobials. serum bactericidal titers were determined and provided additional clinical guidance in the management of such patients. | 2011 | 22162703 |
| Optimization of Culture Conditions for 1,3-Propanediol Production from Glycerol Using a Mutant Strain of Klebsiella pneumoniae. | In the present work, mutant strains of Klebsiella pneumoniae with deletions of the als gene encoding acetolactate synthase involved in synthesis of 2,3-butanediol, the ldhA gene encoding lactate dehydrogenase required for lactate synthesis, or both genes, were prepared. Production of 1,3-propanediol (1,3-PD) from glycerol was enhanced in the ldhA mutant strain (?ldhA), but lower in ?als or ?als ?ldhA mutant strains compared to the parent strain, concomitant with a reduction in the glycerol consu ... | 2011 | 22072138 |
| Function of UreB in Klebsiella aerogenes urease. | Urease from Klebsiella aerogenes is composed of three subunits (UreA-UreB-UreC) that assemble into a (UreABC)(3) quaternary structure. UreC harbors the dinuclear nickel active site, whereas the functions of UreA and UreB remain unknown. UreD and UreF accessory proteins previously were suggested to reposition UreB and increase the level of exposure of the nascent urease active site, thus facilitating metallocenter assembly. In this study, cells were engineered to separately produce (UreAC)(3) or ... | 2011 | 21939280 |
| characterization of purified new delhi metallo-β-lactamase-1. | new delhi metallo-β-lactmase-1 (ndm-1) has recently emerged as a global threat because of its ability to confer resistance to almost all clinically used β-lactam antibiotics, its presence within an easily transmissible plasmid bearing a number of other antibiotic resistance determinants, its carriage in a variety of enterobacteria, and its presence in both nosocomial and community-acquired infections. to improve our understanding of the molecular basis of this threat, ndm-1 was purified and char ... | 2011 | 22029287 |
| CTX-M-9 group extended-spectrum ß-lactamases in neonatal stool isolates: emergence in India. | The study reports for the first time the identification of CTX-M-14-like and CTX-M-27-like extended-spectrum ß-lactamases (ESBLs) belonging to the CTX-M-9 group in Klebsiella pneumoniae and Escherichia coli isolated from the neonatal stool in India. The plasmid carrying the blaCTX-M-9 group in both the isolates was transferable. Till date, no other CTX-M group, except the CTX-M-1 group, has been reported from India. A total of 77% of the neonates had ESBL-producing K. pneumoniae or E. coli in th ... | 2011 | 21860116 |
| [hospital and community-acquired β-lactamases-producing escherichia coli and klebsiella pneumoniae at hospitals in hermosillo, sonora]. | to determine the prevalence of extended-spectrum β-lactamases (esbl)-producing esherichia coli and klebsiella pneumoniae in hospitals of hermosillo, sonora, mexico. | 2011 | 21986791 |
| [incidence, risk factors and outcome of nosocomial pneumonia patients with central nervous system infections]. | pneumonia is the most frequent nosocomial infection in intensive care units. the reported frequency varies with definition, the type of hospital or intensive care units and the population of patients. the incidence ranges from 6.8-27%. | 2011 | 21980657 |
| Simultaneous production of 3-hydroxypropionic acid and 1,3-propanediol from glycerol by a recombinant strain of Klebsiella pneumoniae. | In this study, an aldehyde dehydrogenase (ALDH) was over-expressed in Klebsiella pneumoniae for simultaneous production of 3-hydroxypropionic acid (3-HP) and 1,3-propanediol (1,3-PDO). Various genes encoding ALDH were cloned and expressed in K. pneumoniae, and expression of Escherichia colialdH resulted in the highest 3-HP titer in anaerobic cultures in shake flasks. Anaerobic fed-batch culture of this recombinant strain was further performed in a 5-L reactor. The 3-HP concentration and yield re ... | 2012 | 22055092 |
| [enteric gram negative rods and unfermented of glucose bacteria in patients with peri-implant disease]. | implants can be colonized by microorganisms from oral biofilms and may affect peri-implant tissues health. among these bacteria, pathogens typically associated with periodontitis can be found, such as aggregatibacter actinomycetemcomitans and porphyromonas gingivalis, as well as gram negative enteric bacilli not typically associated with periodontal diseases. | 2011 | 22159480 |
| green fluorescent protein (gfp)-labeling of enterobacteria associated with fruit flies (diptera: tephritidae) and persistence in their natural host rhagoletis completa cresson. | fruit flies (diptera: tephritidae) are a highly successful, widespread group of insects that cause economic damage in agriculture. data available so far on the composition of the bacterial community associated with their digestive tract indicate that members of enterobacteriaceae are the species most often isolated. bacteria naturally occurring in insect guts may be engineered and used to study the spatial and functional interactions of microbes within the insect system and offer one route to me ... | 2011 | 22004066 |
| Occurrence of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae isolates. | Adequate detection of the production of carbapenemase in Enterobacteriaceae isolates is crucial for infection control measures and the appropriate choice of antimicrobial therapy. In this study, we investigated the frequency of false positive results for the detection of carbapenemases in carbapenemase-negative Escherichia coli and Klebsiella pneumoniae clinical isolates by the modified Hodge test (MHT). Three hundred and one E. coli and K. pneumoniae clinical isolates were investigated. All pro ... | 2011 | 22031829 |
| duodenal and faecal microbiota of celiac children: molecular, phenotype and metabolome characterization. | epidemiology of celiac disease (cd) is increasing. cd mainly presents in early childhood with small intestinal villous atrophy and signs of malabsorption. compared to healthy individuals, cd patients seemed to be characterized by higher numbers of gram-negative bacteria and lower numbers gram-positive bacteria. | 2011 | 21970810 |
| Trends in antimicrobial resistance in intensive care units in the United States. | Antimicrobial resistance and a paucity of new antimicrobial agents are ongoing challenges. This review focuses on the major epidemiologic trends and novel treatments, when available, for methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant enterococcus, Clostridium difficile, and multidrug-resistant Gram-negative bacilli in the United States during 2010-2011. | 2011 | 21900766 |
| FDG-PET Quantification of Lung Inflammation with Image-Derived Blood Input Function in Mice. | Dynamic FDG-PET imaging was used to study inflammation in lungs of mice following administration of a virulent strain of Klebsiella (K.) pneumoniae. Net whole-lung FDG influx constant (K(i)) was determined in a compartment model using an image-derived blood input function. Methods. K. pneumoniae (~3 x 10(5) CFU) was intratracheally administered to six mice with 6 other mice serving as controls. Dynamic FDG-PET and X-Ray CT scans were acquired 24 hr after K. pneumoniae administration. The experim ... | 2011 | 22187641 |
| Colistin: recent data on pharmacodynamics properties and clinical efficacy in critically ill patients. | ABSTRACT: Recent clinical studies performed in a large number of patients showed that colistin "forgotten" for several decades revived for the management of infections due to multidrug-resistant (MDR) Gram-negative bacteria (GNB) and had acceptable effectiveness and considerably less toxicity than that reported in older publications. Colistin is a rapidly bactericidal antimicrobial agent that possesses a significant postantibiotic effect against MDR Gram-negative pathogens, such as Pseudomonas a ... | 2011 | 21906382 |
| evaluation of ceftobiprole activity against a variety of gram-negative pathogens, including escherichia coli, haemophilus influenzae (β-lactamase positive and β-lactamase negative), and klebsiella pneumoniae, in a rabbit meningitis model. | ceftobiprole medocaril, a new cephalosporin, is highly active against a broad spectrum of gram-positive and gram-negative clinical pathogens, including methicillin-resistant staphylococcus aureus (mrsa) and penicillin-resistant pneumococci. in this study, we tested ceftobiprole against various gram-negative pathogens in a rabbit meningitis model and determined its penetration into the cerebrospinal fluid (csf). in this animal model, ceftobiprole produced an antibacterial activity similar to that ... | 2011 | 22064544 |
| rapid identification of bovine mastitis pathogens by high-resolution melt analysis of 16s rdna sequences. | accurate identification of mastitis pathogens is often compromised when using conventional culture-based methods. here, we report a novel, rapid assay tested for speciation of bacterial mastitis pathogens using high-resolution melt analysis (hrma) of 16s rdna sequences. real-time pcr amplification of 16s rrna gene fragment, spanning the variable region v5 and v6 was performed with a resulting amplicon of 290bp. first, a library was generated of melt curves of 9 common pathogens that are implicat ... | 2011 | 21944716 |
| risk factors for outbreaks of multidrug-resistant klebsiella pneumoniae in critical burn patients. | the objective of this study is to identify the risk factors related to colonization or infection in an outbreak of multidrug-resistant klebsiella pneumoniae in a burn patient unit. the authors studied the risk factors associated with colonization or infection using a case-control study design involving patients with multidrug resistant k. pneumoniae (n = 26) and controls (n = 50). they describe the outbreak and provide a retrospective analysis that encompasses patient demographics, microbiologic ... | 2011 | 21979841 |
| prevalence and antimicrobial susceptibility pattern of pathogens isolated from patients with juvenile periodontitis in jamaica: a prospective multi-centre study of 15 cases over a 15-year period. | prevalence and antimicrobial susceptibility pattern of most frequent pathogens isolated from patients treated with juvenile periodontitis at three separate dental centres in jamaica from 1989 to 2003 were studied. swabs were taken from these patients periodontal pathologic pocket or root of most of their teeth with active disease processes. these swabs were processed at the microbiology department of the university hospital of the west indies kingston, jamaica and the microbiology laboratory, sc ... | 2011 | 21942137 |
| efficacy of imipenem for the treatment of bacteremia due to an oxa-48-producing klebsiella pneumoniae isolate. | | 2011 | 22157173 |
| Isolation of genes involved in biofilm formation of a Klebsiella pneumoniae strain causing pyogenic liver abscess. | Community-acquired pyogenic liver abscess (PLA) complicated with meningitis and endophthalmitis caused by Klebsiella pneumoniae is an emerging infectious disease. To investigate the mechanisms and effects of biofilm formation of K. pneumoniae causing PLA, microtiter plate assays were used to determine the levels of biofilm formed by K. pneumoniae clinical isolates and to screen for biofilm-altered mutants from a transposon mutant library of a K. pneumoniae PLA-associated strain. | 2011 | 21858144 |
| Recent trends in antibiotic resistance in European ICUs. | Antimicrobial resistance is an emerging problem in ICUs worldwide. As numbers of published results from national/international surveillance studies rise rapidly, the amount of new information may be overwhelming. Therefore, we reviewed recent trends in antibiotic resistance in ICUs across Europe in the past 18 months. | 2011 | 21986462 |
| Emergence of carbapenem-resistant clinical Enterobacteriaceae isolates from a teaching hospital in Shanghai, China. | Carbapenems such as imipenem and meropenem are first-line agents for the treatment of serious nosocomial infections caused by multidrug-resistant clinical isolates of bacteria belonging to the family Enterobacteriaceae. However, resistance to carbapenems has increased dramatically among members of the family Enterobacteriaceae isolated from a teaching hospital in Shanghai, China. In the present study, we investigated the prevalence and molecular characteristics of carbapenem-resistant clinical i ... | 2012 | 21903823 |
| emergence of carbapenem-resistant enterobacteriaceae in austria, 2001-2010. | we report the emergence of carbapenem-resistant enterobacteriaceae in austria. over a 10-year period, carbapenem-resistant enterobacteriaceae isolates were obtained from 13 hospitalized patients, with the first isolation in the year 2005 and a remarkable increase in the number of involved patients in 2010. carbapenem-resistant enterobacteriaceae comprise eight klebsiella pneumoniae isolates, four klebsiella oxytoca isolates, and one escherichia coli isolate. the detected carbapenemases were the ... | 2011 | 21939472 |
| aberrant structures of fecal bacterial community in allergic infants profiled by 16s rrna gene pyrosequencing. | we investigated the correlation between fecal bacteria composition in early infancy and the prevalence of allergic diseases in late infancy. the fecal microbiota in the first 2 months was profiled using the 16s rrna v6 short-tag sequences in the community and statistically compared between two groups of subjects who did and did not show allergic symptoms in the first 2 years (n = 11 vs. 11). in the allergic group, genus bacteroides at 1 month and genera propionibacterium and klebsiella at 2 mont ... | 2011 | 22029688 |
| antimicrobial susceptibility among gram-negative isolates collected from intensive care units in north america, europe, the asia-pacific rim, latin america, the middle east, and africa between 2004 and 2009 as part of the tigecycline evaluation and surveillance trial. | background: the tigecycline evaluation and surveillance trial is an antimicrobial susceptibility surveillance program that collects gram-positive and gram-negative organisms globally. objective: this analysis reports on antimicrobial susceptibility among 23,918 gram-negative isolates collected from intensive care units globally between 2004 and 2009. methods: mics and susceptibility were determined according to the guidelines of the clinical and laboratory standards institute (us food and drug a ... | 2011 | 22154196 |
| Polymyxins: Antimicrobial susceptibility concerns and therapeutic options. | The increasing prevalence of multidrug-resistant nosocomial pathogens such as Acinetobacter baumannii, Pseudomonas aeruginosa and Klebsiella pneumoniae poses a great challenge to the treating physicians. The paucity of newer effective antimicrobials has led to renewed interest in the polymyxin group of drugs, as a last resort for treatment of gram-negative bacterial infections. There is a dearth of information on the pharmacological properties of colistin, leading to difficulties in selecting th ... | 2011 | 21860102 |
| agreement assessment of tigecycline susceptibilities determined by the disk diffusion and broth microdilution methods among commonly encountered resistant bacterial isolates: results from the tigecycline in-vitro surveillance in taiwan [tist] study, 2008-2010. | the tigecycline in-vitro surveillance in taiwan (tist) study, initiated in 2006, is a nationwide surveillance program designed to longitudinally monitor the in-vitro activity of tigecycline against commonly encountered drug-resistant bacteria. this study compared the in-vitro activity of tigecycline against 3,014 isolates of clinically important drug-resistant bacteria using the standard broth microdilution and disk diffusion methods. species studied included methicillin-resistant staphylococcus ... | 2011 | 22155819 |
| screening method for detection of immediate amino acid decarboxylases--producing bacteria implicated in food poisoning. | assessment of amino acid decarboxylase activity can be conducted using tubed broth or plated agar. in this study, the test was carried out in microtitre plates containing lysine, ornithine, arginine, tyrosine, tryptophan, phenylalanine or histidine as biogenic amine precursors. møller decarboxylase base broth (mdb) with or without 1% of a known amino acid were added to wells of a 96 well-microtitre plate. the wells were inoculated with escherichia coli, klebsiella pneumoniae, acinetobacter anitr ... | 2011 | 22041756 |
| turnover and accessibility of a reentrant loop of the na+-glutamate transporter glts are modulated by the central cytoplasmic loop. | glts of escherichia coli is a secondary transporter that catalyzes na+-glutamate symport. the structural model of glts shows two homologous domains with inverted membrane topology that are connected by a central loop that resides in the cytoplasm. each domain contains a reentrant loop structure. accessibility of the cys residues in two glts mutants in which pro351 and asn356 in the reentrant loop in the c-terminal domain were replaced by cys is demonstrated to be sensitive to the catalytic state ... | 2011 | 21995702 |
| urinary tract infection following kidney transplantation: frequency, risk factors and graft function. | the aim of this study was to determine the proportion of children who develop urinary tract infection (uti) after kidney transplantation (ktx) and to identify the factors associated with uti and its impact on graft function. to this end, we undertook a chart review of children who underwent ktx at red cross children's hospital between january 2003 and december 2009 and were followed-up for at least 6 months after transplantation. sixty-two children (53.2% males) were followed-up for a mean (stan ... | 2011 | 22057219 |
| Pilotin-secretin recognition in the type II secretion system of Klebsiella oxytoca. | A crucial aspect of the functionality of bacterial type II secretion systems is the targeting and assembly of the outer membrane secretin. In the Klebsiella oxytoca type II secretion system, the lipoprotein PulS, a pilotin, targets secretin PulD monomers through the periplasm to the outer membrane. We present the crystal structure of PulS, an all-helical bundle that is structurally distinct from proteins with similar functions. Replacement of valine at position 42 in a charged groove of PulS abo ... | 2011 | 22098633 |
| mrkh, a novel c-di-gmp-dependent transcriptional activator, controls klebsiella pneumoniae biofilm formation by regulating type 3 fimbriae expression. | klebsiella pneumoniae causes significant morbidity and mortality worldwide, particularly amongst hospitalized individuals. the principle mechanism for pathogenesis in hospital environments involves the formation of biofilms, primarily on implanted medical devices. in this study, we constructed a transposon mutant library in a clinical isolate, k. pneumoniae aj218, to identify the genes and pathways implicated in biofilm formation. three mutants severely defective in biofilm formation contained i ... | 2011 | 21901098 |
| [Bacteria isolated from surgical infections and its susceptibilities to antimicrobial agents--special references to bacteria isolated between April 2009 and March 2010]. | Bacteria isolated from surgical infections during the period from April 2009 to March 2010 were investigated in a multicenter study in Japan, and the following results were obtained. In this series, 671 strains including 16 strains of Candida spp. were isolated from 174 (79.1%) of 220 patients with surgical infections. Four hundred and eleven strains were isolated from primary infections, and 244 strains were isolated from surgical site infection. From primary infections, anaerobic Gram-negative ... | 2011 | 21861307 |
| Comparison of bacteria isolated from urinary tract infections in patients on transplant versus urologic wards. | Urinary tract infections (UTI) are the most common hospital infections. Complications include sepsis and shock. Immunosuppressed transplant surgery patients may experience loss of the graft due to UTI. The purpose of this study was to determine the main microorganisms responsible for UTIs among patients of transplant wards compared to urologic wards. Additionally, drug susceptibility profiles of the most frequent microorganisms were analyzed. | 2011 | 21996243 |
| dehydratase mediated 1-propanol production in metabolically engineered escherichia coli. | with the increasing consumption of fossil fuels, the question of meeting the global energy demand is of great importance in the near future. as an effective solution, production of higher alcohols from renewable sources by microorganisms has been proposed to address both energy crisis and environmental concerns. higher alcohols contain more than two carbon atoms and have better physiochemical properties than ethanol as fuel substitutes. | 2011 | 22074179 |
| rapid detection of blaimp-6 by amplification refractory mutation system. | klebsiella pneumoniae resistant to almost all ß-lactams except imipenem designated as ismrk (imipenem-susceptible meropenem-resistant klebsiella) is emerging in japan. all ismrk carries bla(imp-6) which differs from bla(imp-1) by only a single nucleotide at position 640. we devised a rapid detection system of bla(imp-6) by using arms pcr. | 2012 | 22108497 |
| inhibition of growth of highly resistant bacterial and fungal pathogens by a natural product. | the continuous escalation of resistant bacteria against a wide range of antibiotics necessitates discovering novel unconventional sources of antibiotics. b. oleracea l (red cabbage) is health-promoting food with proven anticancer and anti-inflammatory activities. however, it has not been researched adequately for its antimicrobial activity on potential resistant pathogens. the methanol crude extract of b. oleracea l. was investigated for a possible anti-microbial activity. the screening method w ... | 2011 | 21915230 |
| role of bacteria isolates in the spoilage of fermented african oil bean seed ugba. | study was carried out to determine the spoilage association of traditionally fermented ugba, product of african oil bean seed. samples were collected from three markets within enugu metropolis in eastern nigeria. microbial population of ugba was isolated, characterized and identified. effect of time on product quality, total viable cell count and individual growth pattern of isolates was evaluated. microorganisms isolated were proteus sp., klebsiella sp., staphylococcus epidermidis, e. coli, bac ... | 2010 | 21848074 |
| Characterization of alginate lyase gene using a metagenomic library constructed from the gut microflora of abalone. | A metagenomic fosmid library was constructed using a genomic DNA mixture extracted from the gut microflora of abalone. The library gave an alginate lyase positive clone (AlyDW) harboring a 31.7-kbp insert. The AlyDW insert consisted of 22 open reading frames (ORFs). The deduced amino acid sequences of ORFs 11-13 were similar to those of known alginate lyase genes, which are found adjacent in the genome of Klebsiella pneumoniae subsp. aerogenes, Vibrio splendidus, and Vibrio sp. belonging to the ... | 2011 | 22072436 |
| The Response of Type 2 Quorum Sensing in Klebsiella pneumoniae to a Fluctuating Culture Environment. | Bacterial cells communicate with one another using chemical signaling molecules. This phenomenon is termed quorum sensing (QS). QS in Klebsiella pneumoniae is mediated by the synthesis of interspecies autoinducer 2 (AI-2), a furanosyl borate diester molecule. The response of Type 2 QS to environmental cues such as carbon sources, the initial pH of the medium, and boracic acid was investigated in the present study using a Vibrio harveyi BB170 reporter assay and quantitative reverse transcription- ... | 2011 | 21877918 |
| CTX-M-15-producing Escherichia coli clone B2-O25b-ST131 and Klebsiella spp. isolates in municipal wastewater treatment plant effluents. | The global occurrence of antibiotic resistance genes in bacteria in water environments is an increasing concern. Treated wastewater was sampled daily over a 45 day period from the outflow of a municipal wastewater treatment plant in Brno, Czech Republic, and examined for extended-spectrum ß-lactamase (ESBL)-producing bacteria. | 2011 | 21954457 |
| in vitro antimicrobial studies of silver carbene complexes: activity of free and nanoparticle carbene formulations against clinical isolates of pathogenic bacteria. | silver carbenes may represent novel, broad-spectrum antimicrobial agents that have low toxicity while providing varying chemistry for targeted applications. here, the bactericidal activity of four silver carbene complexes (sccs) with different formulations, including nanoparticles (nps) and micelles, was tested against a panel of clinical strains of bacteria and fungi that are the causative agents of many skin and soft tissue, respiratory, wound, blood, and nosocomial infections. | 2012 | 21972270 |
| the characteristics of vim-1-producing klebsiella pneumoniae from south africa. | abstract a study was designed to characterize a carbapenem-resistant klebsiella pneumoniae (kpsa01) isolated from a patient in gauteng, south africa without recent travel outside south africa. molecular characterization was done using isoelectric focusing, polymerase chain reaction and sequencing for bla(vim), bla(imp), bla(ndm), bla(ctx-ms), bla(oxas), bla(tems), and bla(shv), plasmid-mediated quinolone resistance determinants, multilocus sequencing typing, plasmid replicon typing, and addict ... | 2012 | 21954935 |
| Plasmid-mediated transfer of the bla(NDM-1) gene in Gram-negative rods. | The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase NDM-1 (New Delhi metallo-ß-lactamase) producers, mostly in Enterobacteriacae. Five bla(NDM) (-1) -positive plasmids of different incompatibility groups (IncL/M, FII, A/C and two untypeable plasmids) from clinical Enterobacteriaceae were evaluated for conjugation properties and host specificity. Successful conjugative transfers were obtained using all tested enterobacterial species as rec ... | 2011 | 22092811 |
| [nosocomial infections in neonatological wards in bulgaria (2000-2009)]. | newborns have a reduced immunological resistance. that fact together with the risk factors, associated with hospitalization, determine the high level of nosocomial infections'illnesses in neonatological wards. the ni incidence of every 100 treated newborns at these wards in bulgaria is 1.99% for the 2000-2009 period. at the neonatological wards in bulgaria the nosocomial sepsis has a frequency of 1 of 1000 released patients. the leading role is that of coagulase-negative staphylococcus (cns) wit ... | 2011 | 21913567 |
| Molecular characterization of CTX-M ß-lactamases among Klebsiella pneumoniae isolated from patients at Tehran hospitals. | Plasmid-encoded CTX-M-group of extended-spectrum ß-lactamases (ESBLs) represent a significant and rapidly emerging problem in most part of the world. The aim of the present study was to describe the prevalence of CTX-M producing Klebsiella pneumoniae at Tehran hospitals. | 2011 | 21860105 |
| Predictors of surgical site infections among patients undergoing major surgery at Bugando Medical Centre in Northwestern Tanzania. | Surgical site infection (SSI) continues to be a major source of morbidity and mortality in developing countries despite recent advances in aseptic techniques. There is no baseline information regarding SSI in our setting therefore it was necessary to conduct this study to establish the prevalence, pattern and predictors of surgical site infection at Bugando Medical Centre Mwanza (BMC), Tanzania. | 2011 | 21880145 |
| six-month incidence of bloodstream infections in intestinal transplant patients. | background: intestinal transplantation has emerged as an established treatment for life-threatening intestinal failure. the most common complication and cause of death is infection. risk of infection is highest during the first 6 months, as a consequence of maximal immunosuppression, greater than that required for any other organ allograft. methods: we performed a retrospective chart review of all (56) adult and pediatric (<18 years) small bowel transplant patients at our institution between nov ... | 2011 | 22093913 |
| antibacterial properties of a glycolipid-rich extract and active principle from nunavik collections of the macroalgae fucus evanescens c. agardh (fucaceae). | this study investigated the antibacterial activity of glycolipid-rich extracts of the brown macroalga fucus evanescens in cell culture. accessions were collected on the arctic coast of ungava bay, nunavik, quebec. the crude ethyl acetate extract of these accessions showed strong antibacterial activity (≥4 log(10) cfu) against hemophilus influenzae , legionella pneumophila , propionibacterium acnes (atcc and clinical isolate), and streptococcus pyogenes at 100 µg/ml. this algal extract inhibited ... | 2011 | 21859295 |