| pcr-based identification of klebsiella pneumoniae subsp. rhinoscleromatis, the agent of rhinoscleroma. | rhinoscleroma is a chronic granulomatous infection of the upper airways caused by the bacterium klebsiella pneumoniae subsp. rhinoscleromatis. the disease is endemic in tropical and subtropical areas, but its diagnosis remains difficult. as a consequence, and despite available antibiotherapy, some patients evolve advanced stages that can lead to disfiguration, severe respiratory impairment and death by anoxia. because identification of the etiologic agent is crucial for the definitive diagnosis ... | 2011 | 21629720 |
| comparative evaluation of boronic acid combined-disk tests for the detection of kpc carbapenemase-producing enterobacteriaceae clinical isolates. | the accurate phenotypic detection of kpc-producing enterobacteriaceae is an increasing necessity worldwide. we evaluated the performance of boronic acid combined-disk tests using as substrate impenem or meropenem and as inhibitor of kpc production 300 μg aminophenylboronic acid (apba), 600 μg apba or 400 μg phenylboronic acid (pba). tests were considered positive when an increase in the growth-inhibitory zone around carbapenem disk with kpc inhibitor was 5 mm or greater of the growth-inhibitory ... | 2011 | 21632901 |
| bacteriology of deep neck abscesses: a retrospective review of 96 consecutive cases. | introduction: this study aimed to review the microbiology of deep neck abscesses and identify the factors that influence their occurrence. methods: a retrospective chart review was done of patients diagnosed with deep neck abscesses at the department of otorhinolaryngology, tan tock seng hospital, singapore between 2004 and 2009. the data of 131 deep neck abscess patients were reviewed, and those with positive pus culture were included in the study. logistic regression was applied to analyse and ... | 2011 | 21633769 |
| [effect of heavy metal ions on triphenyltin enzymatic degradation]. | the influence of different metal ions and different forms of addition on triphenyltin enzymatic degradation was investigated under conditions using enzyme obtained from klebsiella pneumoniae. the objective of this study is to illuminate the mechanism of enzymatic degradation of triphenyltin (tpht). the results demonstrated that the strain was able to tolerate k+, mg2+, cu2+, ca2+ and fe3+ at high concentrations. high concentrations of zn2+ and fe2+ had some toxic effects on the strain, thus affe ... | 2011 | 21634178 |
| carbapenemase-producing klebsiella pneumoniae: (when) might we still consider treating with carbapenems? | clin microbiol infect 2011; 00: 000-000 abstract: infections caused by carbapenemase-producing klebsiella pneumoniae (cpkp) are increasing in frequency worldwide. cpkp isolates exhibit extensive drug resistance phenotypes, complicate therapy, and limit treatment options. although cpkp isolates are often highly resistant to carbapenems, a proportion of these have relatively low mics for carbapenems, raising the question of whether this class of agents has any therapeutic potential against cpkp in ... | 2011 | 21635663 |
| studies on interaction of colloidal silver nanoparticles (snps) with five different bacterial species. | silver nanoparticles (snps) are being increasingly used in many consumer products like textile fabrics, cosmetics, washing machines, food and drug products owing to its excellent antimicrobial properties. here we have studied the adsorption and toxicity of snps on bacterial species such as pseudomonas aeruginosa, micrococcus luteus, bacillus subtilis, bacillus barbaricus and klebsiella pneumoniae. the influence of zeta potential on the adsorption of snps on bacterial cell surface was investigate ... | 2011 | 21640562 |
| klebsiella pneumoniae producing kpc carbapenemase in a district general hospital in the uk. | we report two patients with multidrug-resistant kpc-carbapenemase-producing klebsiella pneumoniae urinary tract infections. a bla(kpc-2) gene was detected in both of the isolates by polymerase chain reaction and sequencing. the isolates had identical pulsed-field gel electrophoresis patterns and belonged to sequence type st11. the index patient probably acquired the kpc-producing strain while in hospital in curaçao, with subsequent nosocomial transmission to the second patient occurring in our h ... | 2011 | 21641083 |
| assessing the effect of oxygen and microbial inhibitors to optimize ferricyanide-mediated bod assay. | methods for short-term bod analysis (bod(st)) based on ferricyanide mediator reduction have succeeded in overcoming some problems associated with the standard bod test analysis (bod(5)) such as long-term incubations (5 days), the need to dilute samples and low reproducibility. here we present a bioassay where a klebsiella pneumoniae environmental strain successfully reduces ferricyanide without de-aeration of the samples with linear bod(5) ranges between 30 and 500mgl(-1) or 30 and 200mgl(-1), u ... | 2011 | 21645725 |
| biofilm formation as a function of adhesin, growth medium, substratum and strain type. | biofilm formation is involved in the majority of bacterial infections. comparing six escherichia coli and klebsiella pneumoniae isolates revealed significant differences in biofilm formation depending on the growth medium. fimbriae are known to be involved in biofilm formation, and type 1, f1c and p fimbriae were seen to influence biofilm formation significantly different depending on strain background, growth media and aeration as well as surface material. altogether, this report clearly demons ... | 2011 | 21646046 |
| expression of a clostridium perfringens type iv pilin by neisseria gonorrhoeae mediates adherence to muscle cells. | clostridium perfringens is an anaerobic, gram-positive bacterium that causes a range of diseases in humans, including lethal gas gangrene. we have recently shown that strains of c. perfringens move across the surface of agar plates using a unique type iv pili (tfp)-mediated social motility that had not been previously described. based on sequence homology to pilins in gram-negative bacteria, c. perfringens appears to have two pilin subunits, pila1 and pila2. structural prediction analysis indica ... | 2011 | 21646450 |
| asymptomatic bacteriuria and antibacterial susceptibility patterns in an obstetric population. | introduction. asymptomatic bacteriuria (asb), occurring in 2-11% of pregnancies, is a major predisposition to the development of pyelonephritis, which is associated with obstetrical complications, such as preterm labor and low birth weight infants. the aim of this study was to determine the prevalence of asb, the antibacterial susceptibilities of the isolated microorganisms and the associated risk factors in an outpatient clinical setting in zekai tahir burak women's health education and researc ... | 2011 | 21647231 |
| synergistic antibacterial effects of polyphenolic compounds from olive mill wastewater. | polyphenols or phenolic compounds are groups of secondary metabolites widely distributed in plants and found in olive mill wastewater (omw). phenolic compounds as well as omw extracts were evaluated in vitro for their antimicrobial activity against gram-positive (streptococcus pyogenes and staphylococcus aureus) and gram-negative bacteria (escherichia coli and klebsiella pneumoniae). most of the tested phenols were not effective against the four bacterial strains when tested as single compounds ... | 2011 | 21647315 |
| the impact of a nationwide antibiotic restriction program on antibiotic usage and resistance against nosocomial pathogens in turkey. | purpose: antimicrobial resistance among microorganisms is a global concern. in 2003, a nationwide antibiotic restriction program (narp) was released in turkey. in this study we evaluated the effect of narp on antibiotic consumption, antimicrobial resistance, and cost.materials and methods: the data obtained from all of the four university hospitals, and one referral tertiary-care educational state hospital in ankara. antimicrobial resistance profiles of 14,233 selected microorganisms all grown i ... | 2011 | 21647326 |
| synthesis of 4-hydroxycoumarin heteroarylhybrids as potential antimicrobial agents. | a new series of 4-hydroxycoumarin derivatives 3a-d was synthesized by the reaction of 3-bromo-4-hydroxy coumarin 1 with various heteroaldehydes 2a-d in good yields. the synthesized compounds were characterized on the basis of their elemental and spectral (ir, (1) h-nmr and mass spectrometry) analysis. all target compounds were evaluated for their in-vitro antimicrobial activity against streptococcus pyogenes, methicillin-resistant staphylococcus aureus, pseudomonas aeruginosa, klebsiella pneumon ... | 2011 | 21647940 |
| [comparison of 2,3-butanediol production by several strains and optimization of the fermentation medium]. | five klebsiella pneumonia strains (including two strains whose genes for lactic acid were knocked out) were used to produce 2,3-butanediol, in which k. pneumonia hr521 ldh (gene for lactic acid was knocked out) was the best for the production, and then the fermentation medium was optimized by orthogonal design. the optimum compositions were as follows: glucose 90 g/l, (nh4)2hpo4 3 g/l, clsp 6 g/l, sodium acetate 5 g/l, kcl 0.4 g/l, mgso4 0.1 g/l, feso4 x 7h2o 0.02 g/l, mnso4 0.01 g/l. under the ... | 2011 | 21650032 |
| microstructured cystine dendrites-based impedimetric sensor for nucleic acid detection. | we report results of the studies relating to the fabrication and characterization of novel biosensing electrode by covalent immobilization of dna onto microstructural cystine (cys) prepared by acoustic cavitation method. the tem investigations of these structures reveal transformation of microstructured cys from nanorods to dendritic structure under optimum conditions. the cys dendrites (dencys) have been investigated by xrd, ft-ir, and sem studies. these biosensing electrodes have been fabricat ... | 2011 | 21650182 |
| country-to-country transfer of patients and the risk of multi-resistant bacterial infection. | management of patients with a history of healthcare contact in multiple countries is now a reality for many clinicians. leisure tourism, the burgeoning industry of medical tourism, military conflict, natural disasters, and changing patterns of human migration may all contribute to this emerging epidemiological trend. such individuals may be both vectors and victims of healthcare-associated infection with multiresistant bacteria. current literature describes intercountry transfer of multiresistan ... | 2011 | 21653302 |
| carbapenem-resistant enterobacteriaceae: epidemiology and prevention. | over the past 10 years, dissemination of klebsiella pneumoniae carbapenemase (kpc) has led to an increase in the prevalence of carbapenem-resistant enterobacteriaceae (cre) in the united states. infections caused by cre have limited treatment options and have been associated with high mortality rates. in the previous year, other carbapenemase subtypes, including new delhi metallo-β-lactamase, have been identified among enterobacteriaceae in the united states. like kpc, these enzymes are frequent ... | 2011 | 21653305 |
| multilocus sequence typing of incn plasmids. | objectives incompatibility group n (incn) plasmids have been associated with the dissemination of antimicrobial resistance and are a major vehicle for the spread of bla(vim-1) in humans and bla(ctx-m-1) in animals. a plasmid multilocus sequence typing (pmlst) scheme was developed for rapid categorization of incn plasmids. methods twelve fully sequenced incn plasmids available at genbank were analysed in silico for selecting the loci for the incn-specific pmlst. a total of 58 plasmids originating ... | 2011 | 21653604 |
| early detection of colonization by vim-1-producing klebsiella pneumoniae and ndm-1-producing escherichia coli in two children returning to france. | rapid identification of metallo-β-lactamase-producing gram-negative species is crucial for the timely implementation of infection control measures. we describe two pediatric cases in which colonization by vim-1- and new delhi metallo-beta-lactamase 1-producing enterobacteriaceae was rapidly detected by phenotypic and genotypic methods. phenotypic methods can be useful for routine detection of carbapenemase production. | 2011 | 21653781 |
| an outbreak of ctx-m-15-producing klebsiella pneumoniae isolates in an intensive care unit of a teaching hospital in kuwait. | this study reports an outbreak of klebsiella pneumoniae infections in 14 patients during a 2-month period (august-september, 2008) in the intensive care unit (icu) of a teaching hospital in kuwait. | 2011 | 21654106 |
| correlation of tem, shv and ctx-m extended-spectrum beta lactamases among enterobacteriaceae with their in vitro antimicrobial susceptibility. | the present study was carried out to characterize the esbl types and evaluated their in vitro activity against a collection of gram negative bacteria (gnb) from a multicentric indian surveillance study. | 2011 | 21654112 |
| endotipsitis: a diagnostic challenge. | | 2011 | 21654138 |
| hemoperfusion treatment in a septic shock patient with autosomal dominant polycystic kidney disease and increased hmgb1 protein levels. | this case report describes polymyxin b-immobilized fiber (pmx-f) treatment of septic shock caused by pyelonephritis in a 68-year-old woman with autosomal dominant polycystic kidney disease. she was admitted for severe lower left abdominal pain, high fever (40°c) and gross hematuria. her endotoxin and high-mobility group box-1 protein (hmgb1) levels were extremely elevated. her blood pressure was 68/36 mm hg. urinalysis revealed innumerable white blood cells (wbcs). blood and urine cultures were ... | 2011 | 21654166 |
| effect of antibiotic diversity on ventilator-associated pneumonia caused by eskape organisms. | the aim of this study was to test in the clinic whether antimicrobial diversity affects resistance of enterococcus faecium, staphylococcus aureus, klebsiella species, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter species (eskape) pathogens in ventilator-associated pneumonia (vap). | 2011 | 21659436 |
| diversity and phylogeny of bacteria on zimbabwe tobacco leaves estimated by 16s rrna sequence analysis. | microorganisms play important roles in the tobacco aging process. however, microbial communities on flue-cured tobacco leaves (fctl) remain largely unknown. in this study, the total microbial genomic dna of unaged and aging fctl from zimbabwe were isolated using a culture-independent method, and the bacterial communities were investigated through analyzing two 16s rrna gene libraries. eighty-four and 65 operational taxonomic units were obtained from the libraries of the unaged and aging fctl, re ... | 2011 | 21660545 |
| addition of genes for cellobiase and pectinolytic activity in escherichia coli for fuel ethanol production from pectin-rich lignocellulosic biomass. | ethanologenic escherichia coli strain ko11 was sequentially engineered to contain the klebsiella oxytoca cellobiose phosphotransferase genes (casab) as well as a pectate lyase (pele) from erwinia chrysanthemi, yielding strains ly40a (casab) and jp07 (casab pele), respectively. to obtain an effective secretion of pele, the sec-dependent pathway out genes from e. chrysanthemi were provided on a cosmid to strain jp07 to construct strain jp07c. finally, oligogalacturonide lyase (ogl) from e. chrysan ... | 2011 | 21666025 |
| institutional control measures to curtail the epidemic spread of carbapenem-resistant klebsiella pneumoniae: a 4-year perspective. | to describe the implementation of an institution-wide, multiple-step intervention to curtail the epidemic spread of carbapenem-resistant klebsiella pneumoniae (crkp). | 2011 | 21666398 |
| [bacteraemia due to escherichia coli producing extended-spectrum beta-lactamases (esbl): clinical relevance and today's insights]. | antibiotic resistance is an old problem with new face as the rate of infections due to multidrug resistant bacteria is higher everyday and the number of new antibiotics to overwhelm the problem is becoming smaller. e. coli is the most frequent agent causing nosocomial or community-acquired bacteraemia being in our country 10% of them extended-spectrum beta-lactamases (esbl) producing e. coli isolates. nowadays the number of community- acquired or health-related infections caused by these esbl pr ... | 2011 | 21666996 |
| microbial pathogens causative of neonatal sepsis in arabic countries. | neonatal sepsis is a major cause of morbidity and mortality worldwide. we analyzed the spectrum of pathogens causing neonatal sepsis in various arabic countries. | 2011 | 21668322 |
| outbreak of kpc-3-producing, and colistin-resistant, klebsiella pneumoniae infections in two sicilian hospitals. | clin microbiol infect 2011; 17: 1444-1447 abstract: we report the first outbreak caused by colistin-resistant klebsiella pneumoniae producing kpc-3 carbapenamase in two italian hospitals. this spread occurred in 1-ámonth, and was caused by eight colistin-resistant and carbapenem-resistant klebsiella pneumoniae isolates from eight patients. a further three isolates were obtained from the intestinal tract and pharyngeal colonization. all isolates were multidrug-resistant (mdr), including being res ... | 2011 | 21668577 |
| pyogenic liver abscess in older adults: comparison with younger individuals. | | 2011 | 21668934 |
| consensus review of the epidemiology and appropriate antimicrobial therapy of complicated urinary tract infections in asia-pacific region. | urinary tract infections (utis) are among the most prevalent infectious diseases in the general population. they cause a substantial financial burden in the community and are associated with significant morbidity and mortality, particularly in hospitals. with increased rates of antimicrobial resistance, especially in the asia-pacific region, treatment of complicated utis (cutis) can be challenging for clinicians. consideration of an optimal antimicrobial agent should be based on local resistance ... | 2011 | 21669223 |
| antimicrobial susceptibilities of commonly encountered bacterial isolates to fosfomycin determined by agar dilution and disk diffusion methods. | we studied the antimicrobial activity of fosfomycin against 960 strains of commonly encountered bacteria associated with urinary tract infection using standard agar dilution and disk diffusion methods. species studied included 3 common species of enterobacteriaceae, pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia; methicillin-susceptible and -resistant staphylococcus aureus; and vancomycin-susceptible and resistant enterococcus faecalis and e. faecium. mics and ... | 2011 | 21670185 |
| susceptibility of klebsiella pneumoniae isolates from intra-abdominal infections and molecular characterization of ertapenem-resistant isolates. | a total of 2,841 clinical isolates of klebsiella pneumoniae from intra-abdominal infections worldwide were collected in the study for monitoring antimicrobial resistance trends (smart) during 2008 and 2009. overall, 22.4% of isolates had extended-spectrum ╬▓-lactamases (esbls). the most active antibiotics among the 11 tested were imipenem, amikacin, and ertapenem, though even these, like all other comparators, were less consistently active against esbl-positive isolates than against esbl-negativ ... | 2011 | 21670192 |
| multiresistance, beta-lactamase-encoding genes and bacterial diversity in hospital wastewater in rio de janeiro, brazil. | aims:ôçé to investigate the bacterial diversity, antimicrobial resistance patterns and types of beta-lactamase genes in gram-negative bacteria isolated from a hospital sewage treatment plant. methods and results:ôçé between july and december 2008, we collected samples from influent, clarifier tank effluent and chlorine contact tank effluent from a sewage treatment plant service of a hospital located in the city of rio de janeiro, brazil. of the 221 isolates identified, 40% were characterized as ... | 2011 | 21672095 |
| synthesis of silver nanoparticles in montmorillonite and their antibacterial behavior. | silver nanoparticles (ag nps) were synthesized by the chemical reducing method in the external and interlamellar space of montmorillonite (mmt) as a solid support at room temperature. agno(3) and nabh(4) were used as a silver precursor and reducing agent, respectively. the most favorable experimental conditions for synthesizing ag nps in the mmt are described in terms of the initial concentration of agno(3). the interlamellar space limits changed little (d-spacing = 1.24-1.47 nm); therefore, ag ... | 2011 | 21674015 |
| hyperbaric oxygen therapy in a mouse model of implant-associated osteomyelitis. | implant associated osteomyelitis (om) is difficult to treat with antibiotics, and outcomes remain poor. some reports suggest that hyperbaric oxygen treatment is a safe and effective means of treating om. we tested this hypothesis in a murine model. clinical isolates of methicillin-resistant staphylococcus aureus (mrsa), pseudomonas aeruginosa, and klebsiella pneumoniae were used. the mice were infected with each of the three pathogens, treated with 100% oxygen at high pressure, hyperbaric oxygen ... | 2011 | 21815206 |
| gastrointestinal colonization with esbl-producing klebsiella in preterm babies-is vancomycin to blame? | in this study, we examine the possible association between treatment with vancomycin and colonization with extended-spectrum beta-lactamase (esbl)-producing klebsiella in our neonatal intensive care unit (nicu). variables compared between newborns which developed rectal colonization and those who did not include: gestational age, birth weight, gender, and total length of hospital stay until positive stool culture or discharge, treatment with vancomycin, and positive blood culture for coagulase-n ... | 2011 | 21814760 |
| incidence, risk stratification, antibiogram of pathogens isolated and clinical outcome of ventilator associated pneumonia. | the initial empirical therapy of ventilator associated pneumonia (vap) modified based on the knowledge of local microbiological data is associated with decreased morbidity and mortality. the objective was to find the incidence and risk factors associated with vap, the implicated pathogens and their susceptibility pattern as well as to assess the final clinical outcome in vap. | 2011 | 21814373 |
| controlled performance evaluation of the diversilab repetitive-sequence-based genotyping system for typing multi-drug resistant healthcare-associated bacterial pathogens. | fast, reliable and versatile typing tools are essential to differentiate among related bacterial strains for epidemiological investigation and surveillance of healthcare-associated infection with multi-drug resistant (mdr) pathogens. the diversilab (dl) system is a semi-automated repetitive sequence-based pcr system designed for rapid genotyping. the dl system performance was assessed by comparing its reproducibility, typeability, discriminatory power, and concordance with pulsed field gel elect ... | 2011 | 21813717 |
| pathogenic microorganisms carried by migratory birds passing through the territory of the island of ustica, sicily (italy). | several studies have shown that migratory birds play an important role in the ecology, circulation and dissemination of pathogenic organisms. in october 2006, a health status evaluation was performed on a large population of migratory birds passing through the territory of ustica (italy), an island located on the migration route of many species of birds to africa, and various laboratory tests were conducted. in total, 218 faecal swabs and the internal organs of 21 subjects found dead in nets wer ... | 2011 | 21812720 |
| [urinary tract infection in patient after brain injury]. | intruduction: one of the most important problems in treating patients after head injury is urinary complication. the first place take urinary infections, which disturb rehabilitation process. clean intermittent catherization, bladder training and good care do not preserve infection. the purpose of our study was to present the frequency of urinary infections and main pathogens involved in these infections among patients treated in the department of rehabilitation after head injury. | 2011 | 21812229 |
| in vitro activity of carbapenems alone and in combination with amikacin against kpc-producing klebsiella pneumoniae. | although carbapenems are the primary treatment strategy for invasive infections caused by esbl bacteria, case reports of these pathogens with reduced carbapenem susceptibility have emerged. one potential treatment modality is to optimize the use of anti-infectives with combination therapy. we evaluated the activity of carbapenems alone and in combination with amikacin against these clinical isolates. | 2011 | 21811540 |
| a vegan or vegetarian diet substantially alters the human colonic faecal microbiota. | background/objectives:consisting of ~10(14) microbial cells, the intestinal microbiota represents the largest and the most complex microbial community inhabiting the human body. however, the influence of regular diets on the microbiota is widely unknown.subjects/methods:we examined faecal samples of vegetarians (n=144), vegans (n=105) and an equal number of control subjects consuming ordinary omnivorous diet who were matched for age and gender. we used classical bacteriological isolation, identi ... | 2011 | 21811294 |
| coexistence of plasmid-mediated kpc-2 and imp-4 carbapenemases in isolates of klebsiella pneumoniae from china. | | 2011 | 21810836 |
| colistin-resistant, klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae belonging to the international epidemic clone st258. | five cases of infection due to colistin-resistant, klebsiella pneumoniae carbapenemase-producing k. pneumoniae belonging to the international epidemic clone st258 occurred over a 4-month period. these cases likely represented both emergence of resistance and transmission of resistant organism. the colistin-resistant isolates were able to persist in the absence of selective pressure in vitro. | 2011 | 21810751 |
| essential oil from aerial parts of of betonica grandiflora willd. from iran. | the chemical composition of the essential oil obtained from the aerial parts of betonica grandiflora willd., growing wild in iran, was analysed by gas chromatography-mass spectrometry for the first time. overall, 40 volatile components were identified on the basis of their mass spectra characteristics and retention indices in which ledol (21.8%), myrtenyl acetate (21.7%), eudesm-7(11)-en-4-ol (6.5%), trans-caryophyllene (5.5%), a-bisabolol (4.9%) and isolongifolol (4.5%) were the major constitue ... | 2011 | 21809955 |
| a call to arms: the imperative for antimicrobial stewardship. | antimicrobial resistance is a major public health crisis. the prevalence of drug-resistant organisms, such as the emerging nap1 strain of clostridium difficile, now highly resistant to fluoroquinolones, acinetobacter species, klebsiella pneumoniae carbapenemase-producing organisms, and methicillin-resistant staphylococcus aureus, is increasing nationwide. the sources of antimicrobial resistance are manifold, but there is a well-documented causal relationship between antimicrobial use and misuse ... | 2011 | 21795727 |
| [common antimicrobial-resistant bacteria in nosocomial infections in taiwan and their prevention]. | infections caused by antibiotic-resistant bacteria, especially multidrug-resistant strains (mdr) that cause difficulties in clinical treatment, have long been a problem of global concern. drug-resistant strains of common nosocomial infections in taiwan include gram-positive coccal methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococci, and gram-negative bacilli such as carbapenem-resistant acinetobacter baumannii, pseudomonas aeruginosa and klebsiella pneumoniae, among ... | 2011 | 21809281 |
| ompk26, a novel porin associated to carbapenem resistance in klebsiella pneumoniae. | clinical isolates of k. pneumoniae resistant to carbapenems are being isolated with increasing frequency. loss of the expression of the major non-specific porins ompk35/36 is a frequent feature in these isolates. in this study, we look for porins that could compensate for the loss of the major porins in carbapenem resistant organisms. comparison of the outer membrane proteins from two k. pneumoniae clinical isogenic isolates susceptible (kpcs-1) and resistant (kpcr-1) to carbapenems revealed the ... | 2011 | 21807980 |
| synergy of fosfomycin with carbapenems, colistin, netilmicin, and tigecycline against multidrug-resistant klebsiella pneumoniae, escherichia coli, and pseudomonas aeruginosa clinical isolates. | fosfomycin represents a potential last-resort treatment option for infections with certain multidrug-resistant (mdr) gram-negative pathogens. we evaluated double-drug combinations of fosfomycin with imipenem, meropenem, doripenem, colistin, netilmicin, and tigecycline for in vitro synergy against 100 mdr klebsiella pneumoniae, escherichia coli, and pseudomonas aeruginosa clinical isolates, using the etest method. synergy was defined as a fractional inhibitory concentration index =0.5. the isolat ... | 2011 | 21805292 |
| synthesis, characterization, theoretical crystal structure, and antibacterial activities of some transition metal complexes of the thiosemicarbazone (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide. | problem statement. in iraq like most third world countries, attempts discovered new antibiotic drugs derived from thiosemicarbazide and its metal complexes and developed the branch of applied in organic chemistry. approach. new (z)-2-(pyrrolidin-2-ylidene)hydrazinecarbothioamide (l) was synthesized in a good yield by the reaction of pyrrolidone with thiosemicarbazide. co(ii), ni(ii), and cu(ii) complexes of (l) were prepared and characterized by ft-ir, uv/visible spectra, (1)hnmr, and chn analys ... | 2011 | 21804771 |
| klebsiella ozaenae cholecystitis. | the authors report a case of klebsiella ozaenae septicemia, cholecystitis and urinary tract infection in a 65-year-old morbidly obese woman with diabetes. klebsiella ozaenae is considered a nonpathogen or colonizer rarely causing septicemia. this is the first reported case of cholecystitis due to this agent. | 2011 | 21804376 |
| antimicrobial susceptibility patterns of clinical isolates of gram-negative bacteria obtained from intensive care units in a tertiary hospital in beijing, china. | in this study we investigated the prevalence of antimicrobial resistance in clinical isolates of gram-negative bacteria obtained from intensive care units (icus) in the people's liberation army (pla) 309 hospital located in beijing, china. between 2007 and 2010, a total of 1949 isolates of gram-negative bacteria were collected and tested using an antibiotic susceptibility assay. a marked decrease was observed in the susceptibility of acinetobacter baumannii to imipenem and amikacin as compared t ... | 2011 | 21803697 |
| longitudinal characterization of acinetobacter baumannii-calcoaceticus complex, klebsiella pneumoniae, and methicillin-resistant staphylococcus aureus colonizing and infecting combat casualties. | drug-resistant acinetobacter baumannii-calcoaceticus complex, klebsiella pneumoniae, and methicillin-resistant staphylococcus aureus colonize and infect combat casualties from iraq and afghanistan. we retrospectively evaluated relatedness, by pulsed-field gel electrophoresis and antibiotic susceptibility testing, of isolates colonizing and infecting casualties over 2 years. colonizing organisms were unrelated to isolates producing later infection in up to 27% of cases; most isolates underwent ch ... | 2011 | 21802175 |
| first isolation and outbreak of oxa-48-producing klebsiella pneumoniae in an irish hospital, march to june 2011. | | 2011 | 21801694 |
| dna capturing machinery through spore-displayed proteins. | aims: the purpose of this study was to develop a general method for the facile development of a new dna biosensor which utilizes streptavidin-displayed spores as a molecular machinery. methods and results: fluorescence spectroscopy was used as a monitoring tool for the streptavidin displayed on the surface of bacillus thuringiensis spores and as a diagnosis method for dna detection. as a proof-of-concept, four pathogenic bacteria including pseudomonas aeruginosa, acinetobacter baumannii, esche ... | 2011 | 21801185 |
| effect of spacer sequences between blactx-m and isecp1 on blactx-m expression. | spacer sequences sized 42 and 127 bp, respectively, between isecp1 insertion sequence and the bla(ctx-m) were observed in klebsiella pneumoniae. however, it is not known whether different spacers in the upstream of bla(ctx-m) and a promoter within the 127 bp spacer will influence cephalosporin resistance. three recombinant plasmids with different spacers and with or without the isecp1 were constructed to compare the bla(ctx-m-3) expression and susceptibility to cephalosporins. our experiment rev ... | 2011 | 21799195 |
| effect of an antibiotic algorithm on the adequacy of empiric antibiotic therapy given by a medical emergency team. | introduction: delayed administration of effective antimicrobial therapy increases mortality in patients with septic shock. empiric antibiotic selection in this setting can be inaccurate. the objective of this study was to determine whether an antibiotic algorithm (aa) tailored to institutional resistance patterns improves the adequacy of antimicrobial therapy. methods: a retrospective review of our rapid response system database was performed. patients with possible sepsis with positive microbio ... | 2011 | 21798704 |
| spread of carbapenem-resistant klebsiella pneumoniae in a tertiary hospital in sao paulo, brazil. | | 2011 | 21798628 |
| [expression of glycerol dehydrogenase gene in escherichia coli by codon optimization]. | to improve expression level of glycerol dehydrogenase gene glda in escherichia coli by means of codon optimization. | 2011 | 21796985 |
| [screening and breeding high 1,3-propanediol producing strains by genome shuffling]. | to improve the tolerance of main metabolites, we used genome shuffling to achieve high 1,3-propanediol producing mutants. | 2011 | 21796981 |
| rising bacterial resistance to common antibiotics in al ain, united arab emirates. | there is a dearth of local information in al amin, united arab emirates about antibiotic resistance patterns. in this retrospective study in a tertiary referral hospital, antibiotic susceptibility results were analysed over the 5-year period 2004-08 and compared with a previous study in the same hospital during 1999-2002. staphylococcus aureus showed a significant decrease in sensitivity to oxacillin from 95.0% in the period 1999-2002 to 84.4% in 2008. sensitivity of acinetobacter spp. to imipen ... | 2011 | 21796964 |
| structure of 2-oxo-3-deoxygalactonate kinase from klebsiella pneumoniae. | in most organisms, efficient d-galactose utilization requires the highly conserved leloir pathway that converts d-galactose to d-glucose 1-phosphate. however, in some bacterial and fungal species alternative routes of d-galactose assimilation have been identified. in the so-called de ley-doudoroff pathway, d-galactose is metabolized into pyruvate and d-glyceraldehyde 3-phosphate in five consecutive reactions carried out by specific enzymes. the penultimate step in this pathway involves the phosp ... | 2011 | 21795809 |
| structural and kinetic insights into the mechanism of 5-hydroxyisourate hydrolase from klebsiella pneumoniae. | the stereospecific oxidative degradation of uric acid to (s)-allantoin has recently been demonstrated to proceed via two unstable intermediates and requires three separate enzymatic reactions. the second step of this reaction, the conversion of 5-hydroxyisourate (hiu) to 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline, is catalyzed by hiu hydrolase (hiuh). the high-resolution crystal structure of hiuh from the opportunistic pathogen klebsiella pneumoniae (kphiuh) has been determined. kphiuh is a h ... | 2011 | 21795808 |
| synthesis and studies of novel 2-(4-cyano-3-trifluoromethylphenyl amino)-4-(quinoline-4-yloxy)-6-(piperazinyl/piperidinyl)-s-triazines as potential antimicrobial, antimycobacterial and anticancer agents. | a series of novel s-triazine analogs were synthesized and characterized by ir, (1)h nmr, (13)c nmr, (19)f nmr spectroscopy and elemental analysis. preliminary screening of target compounds against eight bacteria (staphylococcus aureus, bacillus cereus, escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae, salmonella typhi, proteus vulgaris, shigella flexneria), four fungi (aspergillus niger, aspergillus fumigatus, aspergillus clavatus, candida albicans) and mycobacterium tuberculosis ... | 2011 | 21794959 |
| contribution of mucoviscosity associated gene a (maga) to virulence in experimental klebsiella pneumoniae endophthalmitis. | purpose: endogenous endophthalmitis secondary to klebsiella pneumoniae liver abscess is a blinding infection that is being reported more frequently in the literature. the k1 capsule and maga contribute to virulence of systemic infection in mice, however, little is known about the role of maga in secondary ocular infections. methods: to assess the role of k. pneumoniae capsule in endophthalmitis, we induced experimental endophthalmitis by direct inoculation of 100 cfu of wild type, maga-deficient ... | 2011 | 21791595 |
| heat shock protein 60 from klebsiella pneumoniae protects mononuclear cells from apoptotic cell death induced by dexamethasone. | aims: bacterial heat shock proteins can have anti-apoptotic effects on human cells. we investigated whether enterobacterial hsp60 can protect peripheral blood mononuclear cells (pbmc) from dxm-induced apoptosis and if this effect requires cytoskeleton participation. main methods: anti-apoptotic effect from enterobacterial hsp60 was analyzed by adding these proteins to peripheral mononuclear cells cultures before dxm induction. percentage of apoptotic cells was determined by subg0 peak and tunel ... | 2011 | 21791241 |
| antibacterial efficacy of lytic bacteriophages against antibiotic-resistant klebsiella species. | bacterial resistance to antibiotics is a leading and highly prevalent problem in the treatment of infectious diseases. bacteriophages (phages) appear to be effective and safe alternatives for the treatment of resistant infections because of their specificity for bacterial species and lack of infectivity in eukaryotic cells. the present study aimed to isolate bacteriophages against klebsiella spp. and evaluate their efficacy against antibiotic-resistant species. seventy-two antibiotic-resistant k ... | 2011 | 21789469 |
| safety of pediatric percutaneous liver biopsy performed by interventional radiologists. | : national data suggest that pediatric percutaneous liver biopsy is increasingly being performed by interventional radiologists rather than pediatric gastroenterologists. the objective of the present report is to describe the safety and effectiveness of percutaneous liver biopsy performed by interventional radiologists in a large cohort of children and to compare the results with the existing literature on biopsies performed by pediatric gastroenterologists. | 2011 | 21788763 |
| further increases in carbapenem-, amikacin-, and fluoroquinolone-resistant isolates of acinetobacter spp. and p. aeruginosa in korea: konsar study 2009. | purpose: the increasing prevalence of antimicrobial resistant bacteria has become a serious worldwide problem. the aim of this study was to analyze antimicrobial resistance data generated in 2009 by hospitals and commercial laboratories participating in the korean nationwide surveillance of antimicrobial resistance program. materials and methods: susceptibility data were collected from 24 hospitals and two commercial laboratories. in the analysis, resistance did not include intermediate suscepti ... | 2011 | 21786445 |
| in vitro activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one extracted from marine streptomyces vitsvk5 spp. against fungal and bacterial human pathogens. | background: pharmacological screening and usage of natural products for the treatment of human diseases has had a long history from traditional medicine to modern drugs. the majority of modern drugs are reported to be mostly from natural products. objective: the aim of the present study was to evaluate the inhibitory activity of 5-(2,4-dimethylbenzyl) pyrrolidin-2-one (dmbpo) extracted from marine streptomyces vitsvk5 spp. isolated from sediment samples collected at marakkanam coast of bay of be ... | 2011 | 21782963 |
| design and synthesis of spiro[indole-thiazolidine]spiro[indole-pyrans] as antimicrobial agents. | a series of novel spiro[indole-thiazolidine]spiro[indole-pyran] derivatives were synthesized from n-(bromoalkyl)indol-2,3-diones via monospiro-bisindole intermediates; the two indole nuclei being connected via n-(ch(2))(n)-n linker. synthesized compounds were evaluated for their antimicrobial activities in vitro against three gram-positive bacteria (staphylococcus aureus, bacillus subtilis, and staphylococcus epidermis), four gram-negative bacteria (escherichia coli, pseudomonas aeruginosa, salm ... | 2011 | 21782421 |
| rising incidence of acute prostatitis following prostate biopsy: fluoroquinolone resistance and exposure is a significant risk factor. | objectives: to evaluate the frequency and potential risk factors for infection-related complications after transrectal prostate biopsy and to propose adjustments in current antimicrobial prophylaxis recommendations. methods: during 2008-2010, 107 patients underwent transrectal ultrasound-guided biopsies of the prostate at our institution. charts were reviewed for infection-related complications within 30 days of the procedure. potential risk factors were evaluated, including age, diabetes mellit ... | 2011 | 21782225 |
| late-onset klebsiella oxytoca flap-margin-related corneal ulcer following laser in situ keratomileusis. | a 45-year-old woman developed a spontaneous flap-margin-related corneal ulcer 11 years after the original laser in situ keratomileusis (lasik) procedure. two enhancements had been performed; the most recent was 3 years prior to presentation. cultures were positive for a heavy growth of klebsiella oxytoca. the ulcer responded clinically to topical treatment with fortified cefazolin. eighteen days after presentation, the infiltrate had resolved and the cornea had fully epithelialized. to our knowl ... | 2011 | 21782101 |
| [clinical and economic expedience of ertapenem therapy of complicated urinary tract infection]. | clinical and economic investigation of various antibiotics use in the treatment of complicated urinary tract infection (cuti) was performed under the russian economic environment. the drugs of comparison were ertapenem, ceftriaxone and levofloxacin. direct costs and their structure were shown, and the cost efficiency was calculated. alternative analysis and one-side susceptibility analysis were performed. in complicated urinary tract infections when the major pathogens were escherichia coli, kle ... | 2011 | 21780670 |
| [antimicrobial activity of bacteriocin s760 produced by enterococcus faecium strain lwp760]. | antimicrobial activity of bacteriocin s760 (enterocin) produced by enterococcusfaecium strain lwp760 was studied. bacteriocin s760 is a cationic, hydrophobic, and heat stable peptide with the molecular weight of 5.5 kda and pl of 9.8. enterocin s760 is shown to inhibit in vitro the growth both of sensitive and resistant to antibacterials gramnegative and grampositive bacteria of 25 species. mics of the bacteriocin s760 vary between 0.05-1.6 mg/l for escherichia coli 0157:h117, salmonella typhimu ... | 2011 | 21780664 |
| impact of hfq on global gene expression and virulence in klebsiella pneumoniae. | klebsiella pneumoniae is responsible for a wide range of clinical symptoms. how this bacterium adapts itself to ever-changing host milieu is still a mystery. recently, small non-coding rnas (srnas) have received considerable attention for their functions in fine-tuning gene expression at a post-transcriptional level to promote bacterial adaptation. here we demonstrate that hfq, an rna-binding protein, which facilitates interactions between srnas and their mrna targets, is critical for k. pneumon ... | 2011 | 21779404 |
| [a case of emphysematous gastritis in a patient with end-stage renal disease]. | emphysematous gastritis is a rare infection of the stomach wall with high mortality rate. it is caused by gas forming organisms and may arise by local spread through the mucosa or hematogenous dissemination from distant focus. clinical manifestation includes acute abdomen with systemic toxicity, and diagnosis is based on radiologic demonstration of gas within the gastric wall. treatment should be aimed to cover gram-negative organisms and anaerobes using wide-spectrum intravenous antibiotics, an ... | 2011 | 21778802 |
| high-mobility group protein n2 (hmgn2) inhibited the internalization of klebsiella pneumoniae into cultured bladder epithelial cells. | since bacterial invasion into host cells is an important step in the infection process, using the agents to interfere with bacterial internalization is an attractive approach to block the infection process. in this work, we describe a new, previously unrecognized role of the human cationic host defense peptide hmgn2 during klebsiella pneumoniae infections. our results revealed that the internalization of k. pneumoniae strain 03183 into cultured bladder epithelial cells (t24) was significantly re ... | 2011 | 21778192 |
| clinical predictors of pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor. | objectives: this study was performed to identify risk factors in pseudomonas aeruginosa bacteremia among gram-negative bacterial infections in non-neutropenic patients with solid tumor. methods: a case-control study was performed to identify clinical predictors for p. aeruginosa bacteremia among non-neutropenic patients with gram-negative bacteremia. each case of p. aeruginosa bacteremia was matched to one or two controls with escherichia coli, klebsiella, enterobacter or citrobacter species in ... | 2011 | 21777616 |
| cryptogenic pyogenic liver abscess as the herald of colon cancer. | background and aim: colonic mucosal defects might be a route for bacterial invasion into the portal system with subsequent hematogenous spread to the liver. we retrospectively investigated the results of colonoscopy and clinical characteristics of patients with pyogenic liver abscess of colonic origin. methods: a total of 230 consecutive patients with pyogenic liver abscess were reviewed between 2003 and 2010. the 230 patients were categorized into three groups [pancreato-biliary (n = 135), cryp ... | 2011 | 21777280 |
| longitudinal survey of carbapenem resistance and resistance mechanisms in enterobacteriaceae and non-fermenters from the usa in 2007-09. | background antibiotic resistance is problematic in enterobacteriaceae, pseudomonas aeruginosa and acinetobacter baumannii, and is often associated with serious infections. carbapenems are often one of the few remaining therapeutic options, so it is important to monitor carbapenem activity against these pathogens and to identify resistance mechanisms. methods carbapenem susceptibilities were determined for 14?359 enterobacteriaceae, 3614 p. aeruginosa and 994 a. baumannii from the usa (2007-09). ... | 2011 | 21775338 |
| crystal structure of new delhi metallo-ß-lactamase reveals molecular basis for antibiotic resistance. | ß-lactams are the most commonly prescribed class of antibiotics and have had an enormous impact on human health. thus, it is disquieting that an enzyme called new delhi metallo-ß-lactamase-1 (ndm-1) can confer enterobacteriaceae with nearly complete resistance to all ß-lactam antibiotics including the carbapenams. we have determined the crystal structure of klebsiella pneumoniae apo-ndm-1 to 2.1-å resolution. from the structure, we see that ndm-1 has an expansive active site with a unique electr ... | 2011 | 21774017 |
| methanolic extracts of aloe secundiflora engl. inhibits in vitro growth of tuberculosis and diarrhea-causing bacteria. | the emergence of resistance to antimicrobials by pathogens has reached crisis levels, calling for identification of alternative means to combat diseases. | 2011 | 21772752 |
| preliminary studies for a new antibiotic from the marine mollusk melo melo (lightfoot, 1786). | to search for new bioactive compounds from marine mollusks melo melo (m. melo). | 2011 | 21771476 |
| bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital: high occurrence of combination genes. | to delineate the frequency of occurrence of bla(ctx-m), bla(tem), and bla(shv) in enterobacteriaceae from north-indian tertiary hospital. | 2011 | 21771430 |
| isolation and identification of bioactive antibacterial components in leaf extracts of vangueria spinosa (rubiaceae). | the column chromatographic fraction of ethyl acetate (ea1, ea2, ea3, ea4 and ea5) leaf extracts of vangueria spinosa (v. spinosa) were screened for antibacterial activity and phytochemical analysis. | 2011 | 21771412 |
| transmission of extended-spectrum ß-lactamase klebsiella oxytoca via the breathing circuit of a transport ventilator: root cause analysis and infection control recommendations. | | 2011 | 21768772 |
| production of kpc-2 carbapenemase by an escherichia coli clinical isolate belonging to the international st131 clone. | the rapid international dissemination of klebsiella pneumoniae carbapenemase (kpc) producing organisms is of major concern.… | 2011 | 21768521 |
| emergence of oxa-48-producing escherichia coli clone st38 in france. | the bla(oxa-48) gene encodes a carbapenem-hydrolyzing class d ß-lactamase that was first identified in klebsiella pneumoniae from turkey in 2003 and then extensively in that country (1, 11).… | 2011 | 21768512 |
| characterization of oxa-181, a carbapenem-hydrolyzing class d {beta}-lactamase from klebsiella pneumoniae. | klebsiella pneumoniae kp3 was isolated from a patient, transferred from india to the sultanate of oman. k. pneumoniae kp3 was resistant to all ß-lactams including carbapenems and expressed the carbapenem-hydrolyzing ß-lactamase oxa-181, which differs from oxa-48 by four amino acid substitutions. compared to oxa-48, oxa-181 possessed a very similar hydrolytic profile. the bla(oxa-181) gene was located on a 7.6-kb cole-type plasmid and was linked to insertion sequence isecp1. the isecp1-mediated o ... | 2011 | 21768505 |
| laboratory evaluation of the chromagar kpc medium for identification of carbapenem-nonsusceptible enterobacteriaceae. | chromagar kpc was evaluated with 53 carbapenem-nonsusceptible strains. kpc-producing klebsiella pneumoniae and enterobacter cloacae grew efficiently and yielded metallic blue colonies (100% sensitivity). poor or no growth or atypical colony color were evident with kpc-producing escherichia coli (71.4% sensitivity) and ertapenem-resistant kpc-nonproducing k. pneumoniae (72.7% sensitivity). chromagar kpc appears applicable for processing carbapenem-resistant enterobacteriaceae surveillance specime ... | 2011 | 21767716 |
| detection of synergy using the combination of polymyxin b with either meropenem or rifampin against carbapenemase-producing klebsiella pneumoniae. | polymyxin b (pb) plus meropenem (mer) or rifampin (rif) was tested by etest® method and time-kill assay (tka) against 14 genetically unique clinical klebsiella pneumoniae carbapenemase-producing k. pneumoniae. pb + mer: etest, 43% synergy; tka, 64% synergy. concordance between methods was 79%. for pb + rif: etest, 21% synergy; tka, 100% synergy. concordance between methods was 21%. | 2011 | 21767715 |
| accelerated production of 1,3-propanediol from glycerol by klebsiella pneumoniae using the method of forced ph fluctuations. | 1,3-propanediol (1,3-pd) is a bivalent alcohol, used in a number of chemical syntheses. it could be produced from glycerol in course of microbial fermentation by klebsiella pneumoniae along with more than five minor liquid products. with the purpose to enhance 1,3-pd production and to eliminate by-products formation, principally new ph control on the process was applied. the method, named "forced ph fluctuations" was realized by consecutive raisings of ph with definite ?ph amplitude (ranging fro ... | 2011 | 21766234 |
| functions of some capsular polysaccharide biosynthetic genes in klebsiella pneumoniae ntuh k-2044. | the growing number of klebsiella pneumoniae infections, commonly acquired in hospitals, has drawn great concern. it has been shown that the k1 and k2 capsular serotypes are the most detrimental strains, particularly to those with diabetes. the k1 cps (capsular polysaccharide) locus in the ntuh-2044 strain of the pyogenic liver abscess (pla) k. pneumoniae has been identified recently, but little is known about the functions of the genes therein. here we report characterization of a group of cps g ... | 2011 | 21765903 |
| long-term clinical and economic benefits associated with the management of a nosocomial outbreak resulting from extended-spectrum beta-lactamase-producing klebsiella pneumoniae. | objective:: in 2005, there was an epidemic of infections resulting from extended-spectrum ß-lactamase-producing klebsiella pneumoniae in the intensive care department. the aim of this study was to evaluate the potential long-term clinical and economic benefits resulting from the management of this epidemic and the resulting changes in practices. design:: two periods were defined: the period leading up to and including the epidemic (2003-2005; period i) and the postepidemic period (2006-2008; per ... | 2011 | 21765349 |
| fate of nitrogen-fixing bacteria in crude oil contaminated wetland ultisol. | the effect of crude oil on the growth of legumes (calopogonium muconoides and centrosema pubescens) and fate of nitrogen-fixing bacteria in wetland ultisol was investigated using standard cultural techniques. the results revealed observable effects of oil on soil physico-chemistry, plant growth and nodulation as well as on densities of heterotrophic, hydrocarbonoclastic and nitrogen fixing bacteria. the effects however varied with different levels (0.5%, 1%, 5%, 10%, 15% and 20%) of pollution. a ... | 2011 | 21755289 |
| iliopsoas abscess and spondylodiscitis by staphylococcus aureus: successful clinical treatment. | although pyogenic abscess of the iliopsoas muscle is considered an uncommon condition, its incidence is on the increase. this abscess may be primary if it occurs without a causal factor; more frequently, it is secondary to gastrointestinal or genitourinary affections, or trauma. the main aetiological agents differ among primary (staphylococcus aureus) and secondary (escherichia coli, klebsiella spp, streptococcus spp, bacteroides spp) abscess. we report the case of a 64-year-old man with iliopso ... | 2011 | 21753252 |