| expansion of the known klebsiella pneumoniae species gene pool by characterization of novel alien dna islands integrated into tmrna gene sites. | klebsiella pneumoniae is an important bacterial pathogen of man that is commonly associated with opportunistic and hospital-associated infections. increasing levels of multiple-antibiotic resistance associated with this species pose a major emerging clinical problem. this organism also occurs naturally in other diverse environments, including the soil. consistent with its varied lifestyle and membership of the enterobacteriaceae family, k. pneumoniae genomes exhibit highly plastic architecture c ... | 2010 | 21182879 |
| proposal of protocols using d-glutamine to optimize the 2,3-bis(2-methoxy-4-nitro-5-sulfophenly)-5-[(phenylamino)carbonyl]-2h-tetrazolium hydroxide (xtt) assay for indirect estimation of microbial loads in biofilms of medical importance. | due to technical problems, biofilm biomasses are difficult to be precisely determined. one reliable strategy is based on the colorimetry of formazan compounds derived from tetrazolium salt reduction. xtt presents some desirable properties that make the biofilm measurements easier. however, cells entrapped within the extracellular matrixes normally do not metabolize the tetrazolium equally, leading to underestimation of cell contents. this study evaluated the effectiveness of d-glutamine, a plero ... | 2010 | 21182880 |
| extended-spectrum beta-lactamase-producing gram-negative bacteria causing neonatal sepsis in india in rural and urban settings. | extended-spectrum β-lactamase (esbl)-producing gram-negative bacilli (gnb) are of increasing clinical concern in all age groups worldwide. whilst sepsis continues to be the leading cause of morbidity and mortality in indian neonates in the community, identification of microbiological attributes in this population is lacking. this population-based study enrolled 1738 infants with a diagnosis of clinical sepsis at four participating centres in india. each study site conducted bactec blood culture, ... | 2010 | 21183602 |
| antimicrobial resistance among producers and non-producers of extended spectrum beta-lactamases in urinary isolates at a tertiary hospital in tanzania. | abstract: | 2010 | 21184671 |
| bactericidal oncocin derivatives with superior serum stabilities. | the proline-rich antimicrobial peptide oncocin is remarkably active in vitro against a number of important gram-negative bacteria of concern to humans owing to their increasing resistance to antibiotics, i.e. enterobacteriaceae (escherichia coli, klebsiella pneumoniae and enterobacter cloacae) and non-fermenting species (acinetobacter baumannii and pseudomonas aeruginosa). degradation of oncocin in mouse serum was investigated in this study. several approaches to stabilise the main cleavage site ... | 2010 | 21185160 |
| n15: the linear phage-plasmid. | the lambdoid phage n15 of escherichia coli is very unusual among temperate phages in that its prophage is not integrated into chromosome but is a linear plasmid molecule with covalently closed ends. upon infection the phage dna circularises via cohesive ends, then phage-encoded enzyme, protelomerase, cuts at an inverted repeat site and forms hairpin ends (telomeres) of the linear plasmid prophage. replication of the n15 prophage is initiated at an internally located ori site and proceeds bidirec ... | 2010 | 21185326 |
| clinical characteristics and therapeutic outcome of gram-negative bacterial spinal epidural abscess in adults. | gram-negative (g(-)) bacterial spinal epidural abscess (sea) in adults is uncommon. of the 42 adult patients with bacterial sea admitted to the chang gung memorial hospital - kaohsiung, between 2003 and 2007, 12 with g(-) sea were included in this study. of these 12 patients, seven were men and five were women; their ages ranged between 17 years and 81 years (median=72.5 years, mean=62.5 years). the patients were admitted at different stages of symptom onset (four were in the acute stage and fou ... | 2010 | 21185728 |
| efficient production of ethanol from crude glycerol by a klebsiella pneumoniae mutant strain. | a mutant strain of klebsiella pneumoniae, termed gem167, was obtained by γ irradiation, in which glycerol metabolism was dramatically affected on exposure to γ rays. levels of metabolites of the glycerol reductive pathway, 1,3-propanediol (1,3-pd) and 3-hydroxypropionic acid (3-hp), were decreased in the gem167 strain compared to a control strain, whereas the levels of metabolites derived from the oxidative pathway, 2,3-butanediol (2,3-bd), ethanol, lactate, and succinate, were increased. notabl ... | 2010 | 21186120 |
| characterization and cloning of oxygen-tolerant hydrogenase from klebsiella oxytoca hp1. | hydrogenase from a hot spring bacterium klebsiella oxytoca hp1 was purified and found to have a specific activity of 199.8 u/mg of protein and a yield of 7.3%. the purified enzyme was determined to consist of six subunits (65, 33, 28, 23, 21 and 18 kda), similar to hydrogenase-3 from escherichia coli, and therefore it was named hyd3. the enzyme displayed remarkable oxygen tolerance. for the purified enzyme, 50% maximal activity was maintained following incubation for 24 h in air at room temperat ... | 2010 | 21187144 |
| medicinal plants used in wound care: a study of prosopis africana (fabaceae) stem bark. | the effects of the methanol extract of the stem bark of prosopis africana (guill., perrott. and rich.) taubert (fabaceae) on bleeding/clotting and coagulation time, excision and dead space wounds were studied in rats. also, the extract was subjected to antibacterial, and acute toxicity and lethality (ld(50)) tests. the extract significantly (p<0.05) reduced bleeding/clotting and coagulation time in rats. it also reduced epithelialization period of excision wounds in rats and inhibited the growth ... | 2010 | 21188042 |
| early dissemination of ndm-1- and oxa-181-producing enterobacteriaceae in indian hospitals: report from the sentry antimicrobial surveillance program, 2006-2007. | among 39 carbapenem-resistant enterobacteriaceae (2.7% overall; escherichia coli, enterobacter cloacae, and klebsiella pneumoniae strains) isolated in 2006 and 2007 in india, 15 strains carried bla(ndm-1) and 10 harbored a gene encoding a variant of the carbapenemase oxa-48, named bla(oxa-181). one e. cloacae strain harbored bla(vim-6), and one k. pneumoniae strain carrying bla(oxa-181) also possessed bla(vim-5). multiple pulsed-field gel electrophoresis patterns and clonal dissemination within ... | 2010 | 21189345 |
| comparative evaluation of bactericidal potential of four root canal filling materials against microflora of infected non-vital primary teeth. | since complete debridement of the root canals of the primary teeth is not practically possible due to the highly variable root canal anatomy, success of the endodontic therapy depends partly on the use ofantibacterial irrigating agents and root canal filling materials. recent literature indicates that anaerobes comprise a majority of the bacteria in necrotic root canals ofprimary teeth. the study determined the antibacterial effectiveness of four root canal filling materials namely calcium hydro ... | 2010 | 21189760 |
| persistence and spread of qnr, extended-spectrum beta-lactamase, and ampc resistance genes in the digestive tract of chickens. | the aim of this assay was to develop an experimental model of digestive colonization of chickens with bacteria harboring qnr, extended-spectrum beta-lactamase, or ampc genes. specific pathogen-free chickens were orally inoculated with two escherichia coli strains containing either the plasmid pmg252 bearing bla(fox) and qnra genes, or pmg298 bearing bla(ctx-m) and qnrb genes. analysis of strains isolated from fecal samples showed that the two strains were able to persist for several weeks in the ... | 2010 | 21190475 |
| the non-conventional mhc class i mr1 molecule controls infection by klebsiella pneumoniae in mice. | as opposed to the well established role of mhc-linked, polymorphic, class i (mhc-i) genes in adaptive immunity, a universal role for non-conventional mhc-i is unknown, thus requiring a case-by-case study. the mhc unlinked, monomorphic, but β₂microglobulin (β₂m)-associated "mhc class i related" mr1 molecule interacts with a semi-invariant tcr. the pathophysiology of this interaction or more importantly of this peculiar mhc-i remains mostly unknown. recently it was shown that β₂m deficient mice we ... | 2010 | 21190736 |
| antimicrobial activity of erythromycin and clarithromycin against clinical isolates of escherichia coli, staphylococcus aureus, klebsiella and proteus by disc diffusion method. | fifty clinical isolates comprising of escherichia coli, staphylococcus aureus, klebsiella and proteus were collected from different local pathological laboratories and their resistant pattern against two well known macrolides; erythromycin and clarithromycin were studied using disc diffusion method. klebsiella (41.67% against erythromycin and 58.34% against clarithromycin) and proteus (66.67% against erythromycin and clarithromycin) species were found to be more resistant against the studied mac ... | 2011 | 21190914 |
| klebsiella pneumoniae strains producing extended-spectrum beta-lactamases in spain: microbiological and clinical features. | extended-spectrum β-lactamases (esbl) of the ctx-m, shv, and tem families were recognized in 76 (67%), 31 (27%), and 6 (5%) isolates, respectively, among 162 esbl-producing klebsiella pneumoniae (esbl-kp) strains obtained in a multicenter study in spain. predisposing factors for esbl-kp acquisition included invasive procedures, mechanical ventilation, and previous antimicrobial use. | 2010 | 21191059 |
| early diagnosis and therapeutic choice of klebsiella pneumoniae liver abscess. | nowadays, pyogenic liver abscess (pla) is still a common and severe intra-abdominal infection, and klebsiella pneumoniae had emerged as the most common pathogenic bacteria worldwide in the past ten years. our study aims to achieve an early pathogenic diagnosis and rational therapy modality for klebsiella pneumoniae liver abscess (kla) through clinical data analysis. a total of 197 inpatients in zhongshan hospital, shanghai, diagnosed as having liver abscess between march 2001 and september 2009 ... | 2010 | 21191837 |
| [prevalence of esbl-positive enterobacteriaceae in large moravian hospitals (czech republic)]. | bacterial infections have become an important issue in current medicine. recently, their frequency and severity have significantly increased as a result of the rising number of resistant bacteria. one of important mechanisms of resistance is production of broad-spectrum beta-lactamases, namely the esbl type. the study aimed at determining the frequency of esbl-positive enterobacteriaceae in three large hospitals in moravia, the eastern part of the czech republic. | 2010 | 21191872 |
| new delhi metallo-β-lactamase in klebsiella pneumoniae and escherichia coli, canada. | multidrug-resistant klebsiella pneumoniae and escherichia coli isolates harboring new delhi metallo-β-lactamase (ndm-1) were isolated from a patient who had returned to canada from india. the ndm-1 gene was found on closely related incompatibility group a/c type plasmids. the occurrence of ndm-1 in north america is a major public health concern. | 2011 | 21192866 |
| emergence of new delhi metallo-β-lactamase, austria. | | 2011 | 21192874 |
| plasmid-mediated carbapenem-hydrolysing β-lactamase kpc-2 in a klebsiella pneumoniae isolate from switzerland. | | 2010 | 21193473 |
| genetic and phenotypic diversity of plant growth promoting rhizobacteria isolated from sugarcane plants growing in pakistan. | bacteria were isolated from roots of sugarcane varieties grown in the fields of punjab. they were identified by using api20e/ne bacterial identification kits and from sequences of 16s rrna and amplicons of the cpn60 gene. the majority of bacteria were found to belong to the genera of enterobacter, pseudomonas, and klebsiella, but members of genera azospirillum, rhizobium, rahnella, delftia, caulobacter, pannonibacter, xanthomonas, and stenotrophomonas were also found. the community, however, was ... | 2010 | 21193815 |
| mutations in the gyrb, parc, and pare genes of quinolone-resistant isolates and mutants of edwardsiella tarda. | the full length genes gyrb (2,415 bp), parc (2,277 bp), and pare (1,896 bp) in edwardsiella tarda were cloned by pcr with degenerate primers based on the sequence of the respective quinolone resistance-determining region (qrdr), followed by elongation of 5' and 3' ends using cassette ligation-mediated pcr (clmp). analysis of the cloned genes revealed open reading frames (orfs) encoding proteins of 804 (gyrb), 758 (parc), and 631 (pare) amino acids with conserved gyrase/topoisomerase features and ... | 2010 | 21193831 |
| effectiveness of a new decolonisation regimen for eradication of extended-spectrum β-lactamase-producing enterobacteriaceae. | gram-negative bacteria expressing extended-spectrum β-lactamases (esbl) have emerged worldwide. esbl colonisation can persist for years and may favour esbl transmission. interventions include contact isolation precautions and restriction of antibiotic use, but decolonisation (dc) for esbl is not established. we performed a prospective controlled open-label cohort-study from 1/2000 to 1/2008 to determine the effectiveness of a standardised dc programme. esbl-positive patients routinely underwent ... | 2010 | 21194789 |
| [bacterial flora in acute exacerbations of chronic obstructive pulmonary disease (copd) in kunming, china]. | chinese recommendations for the management of acute exacerbations of chronic obstructive pulmonary disease (aecopd) include antibiotic treatments targeting the most frequently isolated pathogens according to the severity of copd. the study objective was to check the relevance of these recommendations in yunnan, a southern chinese province. | 2010 | 21195565 |
| fever/clinical signs and association with neutropenia in solid cancer patients--bacterial infection as the main cause. | neutropenia remains one of the serious side effects of chemotherapeutics drugs making cancer patients face serious risk of infections. fever and clinical signs are considered as important indicators. the objectives of this study were to assess fever and clinical signs with neutropenia onset and/ or severity in solid cancer cases, using culture tests to determine the type of bacteria predominating, whether gram positive or gram negative. | 2010 | 21198276 |
| rhinoscleroma: an updated experience through the last 10 years. | rhinoscleroma is a chronic, specific, granuloma of the nose and other parts of the respiratory system. the disease is endemic in egypt and many other countries. the causative organism is klebsiella rhinoscleromatis bacillus, proved by fulfilling koch's postulates. the mode of infection is not known and its worldwide irregular geographical distribution is not understood. lines of treatment are unsatisfactory and a tendency for recurrence is the rule. | 2011 | 21198342 |
| purification and properties of antimicrobial peptides from skin secretions of the eritrea clawed frog xenopus clivii (pipidae). | five peptides with antimicrobial activity were isolated from norepinephrine-stimulated skin secretions of the tetraploid frog xenopus clivii peracca, 1898 (pipidae). characterization of the peptides demonstrated that they are structurally similar to magainins (2 peptides), caerulein-precursor fragments, cpf (2 peptides), and xenopsin-precursor fragments, xpf (1 peptide) that have been previously isolated from other species of the genus xenopus. the magainins and the xpf peptide were active only ... | 2011 | 21199684 |
| detection of klebsiella pneumoniae carbapenemase (kpc) production in non-klebsiella pneumoniae enterobacteriaceae isolates by use of the phoenix, vitek 2, and disk diffusion methods. | in this study, we tested the abilities of the vitek 2, bd phoenix, and kirby bauer disk diffusion tests to detect carbapenemase production in a collection of 14 klebsiella pneumoniae carbapenemase (kpc)-producing non-klebsiella pneumoniae isolates. in addition, we evaluated 13 kpc-positive k. pneumoniae isolates by using each of these methods and applied both 2009 and 2010 clsi carbapenem interpretive guidelines. | 2011 | 21209164 |
| inhibition of klebsiella pneumoniae dnab helicase by the flavonol galangin. | klebsiella pneumoniae is a ubiquitous opportunistic pathogen that colonizes at the mucosal surfaces in humans and causes severe diseases. many clinical strains of k. pneumoniae are highly resistant to antibiotics. here, we used fluorescence quenching to show that the flavonols galangin, myricetin, quercetin, and kaempferol, bearing different numbers of hydroxyl substituent on the aromatic rings, may inhibit dntp binding of the primary replicative dnab helicase of k. pneumoniae (kpdnab), an essen ... | 2011 | 21210194 |
| evaluation of three phenotypic identification systems for clinical isolates of raoultella ornithinolytica. | raoultella spp. have recently been separated from the genus klebsiella based on their molecular characteristics. it was discovered that raoultella ornithinolytica can be misidentified as klebsiella oxytoca by commonly used phenotypic identification systems. therefore, this study evaluated the ability of three phenotypic systems to identify r. ornithinolytica compared with the genotypic methods sequence-specific primer pcr (ssp-pcr), 16s rrna gene sequence analysis using the microseq 500 system16 ... | 2011 | 21212145 |
| volatile compounds and antimicrobial and antioxidant activities of the essential oils of the needles of pinus densiflora and pinus thunbergii. | to investigate the volatile compounds and the antibacterial and antioxidant effects of the essential oils of pinus densiflora needles (epdn) and pinus thunbergii needles (eptn), the volatile compounds of steam-distilled essential oils were analysed by gas chromatography-mass spectrometry. antibacterial activities were analysed by performing disc-agar diffusion assay and determining the minimum inhibitory concentrations (mics) of the essential oils. antioxidant activities were analysed via radica ... | 2011 | 21213230 |
| chemical composition and biological activity of extracts from arrabidaea bilabiata. | consumption of arrabidaea bilabiata fresh leaves produces a paraplegic syndrome in cattle. for isolation of the active principle, isopropanol and methanol extracts were prepared from the aerial parts of the plant; these were administered to sprague-dawley rats at doses of 0.5, 1, and 2 mg/kg, orally, to determine the presence of substances affecting spontaneous motor activity. the isopropanol extract produced significant increases of long-pause and short-pause movements (112 and 54%, respectivel ... | 2000 | 21214478 |
| occurrence of kpc-2-producing klebsiella pneumoniae strains in hospital wastewater. | | 2011 | 21216031 |
| inactivation and surface interactions of ms-2 bacteriophage in a tio2 photoelectrocatalytic reactor. | inactivation of ms-2 bacteriophage in a tio(2) photoelectrocatalytic system was evaluated, wherein tio(2) particles were coated onto an indium tin oxide (ito) electrode and an electrical potential was applied under black light blue (blb) irradiation. ms-2 phage inactivation was greatly enhanced by anodic potential, whereas cathodic potential completely inhibited inactivation. experiments performed with radical scavengers showed that inactivation was primarily caused by hydroxyl radicals, both in ... | 2010 | 21216427 |
| septal rhinoscleroma. | rhinoscleroma is a chronic granulomatous condition of the nose and other structures of the upper respiratory tract. infection by the bacterium klebsiella rhinoscleromatis is said to be the cause. a female patient aged 45 years, presented with a past history of trauma to the nose and swelling on her nose since last 1 year. there was nasal asymmetry and internal nasal examination showed a septal swelling protruding to the right nasal cavity with hypertrophied nasal mucosa and inferior turbinate. o ... | 2010 | 21217987 |
| antimicrobial activity of α-(2-hydroxy-2-methylpropyl)-ω-(2-hydroxy-3-methylbut-2-en-1-yl) polymethylene from caesalpinia bonducella (l.) flem. | the compound, α-(2-hydroxy-2-methylpropyl)-ω-(2-hydroxy-3-methylbut-2-en-1-yl)polymethylene, isolated from ethyl acetate leaf extract of caesalpinia bonducella (l.) flem. was evaluated for antimicrobial activity against clinical isolates, proteus vulgaris, pseudomonas aeruginosa, klebsiella sp., staphylococcus citrus, staphylococcus aureus, escherichia coli, candida albicans and rhodotorula sp. using agar diffusion method. the compound exerted inhibitory zone at all concentrations and revealed t ... | 2010 | 21218063 |
| kpc-2 carbapenemase-producing klebsiella pneumoniae isolates from patients with cystic fibrosis. | bacteria producing klebsiella pneumoniae carbapenemases (kpcs) are rapidly emerging as a cause of multidrug-resistant infections worldwide. kpcs enzyme are plasmid-borne and can accumulate and transfer resistance determinants to other classes of antibiotics.we report two cases of kpc-2 carbapenemase-producing klebsiella pneumoniae isolates from cystic fibrosis patients. | 2011 | 21220216 |
| new delhi metallo-β-lactamase-1 in enterobacteriaceae: emerging resistance. | | 2011 | 21220461 |
| role of hydrogen generation by klebsiella pneumoniae in the oral cavity. | some gastrointestinal bacteria synthesize hydrogen (h(2)) by fermentation. despite the presence of bactericidal factors in human saliva, a large number of bacteria also live in the oral cavity. it has never been shown that oral bacteria also produce h(2) or what role h(2) might play in the oral cavity. it was found that a significant amount of h(2) is synthesized in the oral cavity of healthy human subjects, and that its generation is enhanced by the presence of glucose but inhibited by either t ... | 2010 | 21221934 |
| public perception about drinking jar water and its bacteriological analysis. | the consumption of jar water has been increasing consistently in these days. to improve such water quality and supply, information is needed to assess water contamination in a variety of community, including those that rely primarily on unimproved distributed sources of drinking water. this study was done to assess the public perception on drinking jar water and assessment of drinking jar water distributed in kathmandu valley which was conducted in the department of microbiology, amrit science c ... | 2010 | 21222409 |
| surgical treatment of children with necrotizing pneumonia. | to describe the results of the surgical treatment of children with necrotizing pneumonia. | 2010 | 21225174 |
| rhinoscleroma: a case report. | rhinoscleroma is a chronic inflammatory disease in which granulation tissue with a typical cell content is found. the paper presents the case of a 77-year-old woman with clinically diagnosed nodule in the nasal cavity. the histopathological examination revealed granulation tissue with plasma cells and mikulicz's cells. the clinical and morphological picture of the case in question is a rare opportunity to bring to mind a disease that used to be common in poland and which clinically can imitate m ... | 2010 | 21225500 |
| [prevalence of nosocomial infections in 27 hospitals in the mediterranean region]. | a multicentre study was conducted in 27 hospitals in algeria, egypt, italy, morocco and tunisia to evaluate the prevalence and characteristics of the nosocomial infections. the study population (4634 patients) was relatively young, mean age 41.1 (standard deviation 23.4) years. the prevalence of nosocomial infections was 10.5%; this was higher in non-teaching centres and moderate-sized hospitals. overall, urinary tract infections were the most common. paediatric departments rated particularly hi ... | 2010 | 21226344 |
| the effectiveness of a health-surveillance program for caisson saturation divers in a tunnel-boring machine: a microbiological survey. | the purpose of this field study is to report and evaluate the implementation of a health surveillance program we developed to monitor the microbiological load for saturation divers, including preventive and therapeutic interventions. we extended the dmac protocol for saturation diving chamber hygiene and added some components: ear inspections, swabs and environmental swabs every third day. the implementation was evaluated by analyzing the results of the activities. in a pre-saturation dive check ... | 2010 | 21226394 |
| [effects of chinese herbal medicine dusuqing granule on toll-like receptor 4 signaling in multiple organ injury induced by bacterial pneumonia in aged rats]. | to study the protective mechanism of dusuqing granule, a compound chinese herbal medicine, on the senile multiple organ injury caused by bacterial pneumonia by observing the expression changes of molecules related to toll-like receptor 4 (tlr4) signaling. | 2011 | 21227038 |
| the iκb family member bcl-3 coordinates the pulmonary defense against klebsiella pneumoniae infection. | bcl-3 is an atypical member of the iκb family that has the potential to positively or negatively modulate nuclear nf-κb activity in a context-dependent manner. bcl-3's biologic impact is complex and includes roles in tumorigenesis and diverse immune responses, including innate immunity. bcl-3 may mediate lps tolerance, suppressing cytokine production, but it also seems to contribute to defense against select systemic bacterial challenges. however, the potential role of bcl-3 in organ-specific ho ... | 2011 | 21228348 |
| improving turnaround time for identifying extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae: experience with the bd phoenix automated system. | | 2011 | 21233294 |
| combined bone and soft-tissue augmentation surgery in temporo-orbital contour reconstruction. | temporal hollowing due to temporal muscle atrophy after standard skull base surgery is common. various techniques have been previously described to correct the disfiguring defect. most often reconstruction is performed using freehand molded polymethylmethacrylate cement. this method and material are insufficient in terms of aesthetic results and implant characteristics. we herein propose reconstruction of such defects with a polyetheretherketone (peek)-based patient-specific implant (psi) includ ... | 2011 | 21233740 |
| antimicrobial activity of some new of 2-(4-ethyl-phenoximethyl) benzoic acid thioureides against planktonic cells. | the aim of the present study was to evaluate the antimicrobial activity of new 2-(4-ethyl-phenoxymethyl) benzoic acid thioureides. the synthesis of the new compounds was done in three steps starting with the synthesis of 2-(4-ethyl-phenoxymethyl) benzoic acid. in the second stage, the 2-(4-ethyl-phenoxymethyl)-benzoyl chloride was prepared and the new thioureides were synthesized in the third step by the reaction of 2-(4-ethyl-phenoxymethyl) benzoyl isothiocyanate with various primary aromatic a ... | 2010 | 21235136 |
| carijodienone from the octocoral carijoa multiflora. a spiropregnane-based steroid. | two new steroids (1 and 2) and the known pregna-1,4,20-trien-3-one (3) have been isolated from the pacific octocoral carijoa multiflora. compound 1 possesses a novel spiropregnane-based steroidal skeleton. the photochemical transformation of 3 into 1 allowed the assignment of the absolute configuration at c-10 of 1. the antibacterial activities of compounds 1 and 3 were evaluated against a panel of bacterial strains. | 2011 | 21235217 |
| first report of plasmid-mediated quinolone resistance qnra1 gene in klebsiella pneumoniae isolate of animal origin. | one qnra1-producing klebsiella pneumoniae isolate gdka1 from chicken was detected. the qnra1 gene on plasmid pgdka1 was located in a genetic environment similar to that in in36 on plasmid phsh1 and could be cotransferred to escherichia coli j53 az(r) with other resistances by a conjugation experiment. upstream of the qnra1 gene, there was a class i integron with the dfra27 and aada2 cassettes. similar genetic environments of qnra1 in enterobacteriaceae isolates from both human and animal origin ... | 2011 | 21235404 |
| activity of tigecycline alone and in combination with colistin and meropenem against klebsiella pneumoniae carbapenemase (kpc)-producing enterobacteriaceae strains by time-kill assay. | antibiotic combinations including tigecycline have not been studied against klebsiella pneumoniae carbapenemase (kpc)-producing pathogens. tigecycline alone and combined with colistin and meropenem was tested against eight genetically unrelated kpc-producing clinical strains of enterobacteriaceae (four k. pneumoniae, two escherichia coli, one enterobacter cloacae and one serratia marcescens) by time-kill assay. tigecycline displayed a concentration-independent bacteriostatic activity in seven st ... | 2011 | 21236643 |
| on the detection of plasmidic ampc in enterobacteriaceae other than escherichia spp. and klebsiella spp. | | 2011 | 21237619 |
| contribution of the klebsiella pneumoniae capsule to bacterial aggregate and biofilm microstructures. | we studied the interaction between capsule production and hydrodynamic growth conditions on the internal and macroscopic structure of biofilms and spontaneously formed aggregates of klebsiella pneumoniae. wild-type and capsule-deficient strains were studied as biofilms and under strong and mild hydrodynamic conditions. internal organization of multicellular structures was determined with a novel image-processing algorithm for feature extraction from high-resolution confocal microscopy. measures ... | 2011 | 21239544 |
| structural and mechanical properties of klebsiella pneumoniae type 3 fimbriae. | this study investigated the structural and mechanical properties of klebsiella pneumoniae type 3 fimbriae, which constitute a known virulence factor for the bacterium. transmission electron microscopy and optical tweezers were used to understand the ability of the bacterium to survive flushes. an individual k. pneumoniae type 3 fimbria exhibited a helix-like structure with a pitch of 4.1 nm and a three-phase force-extension curve. the fimbria was first nonlinearly stretched with increasing force ... | 2011 | 21239584 |
| nationwide survey of antibacterial activity against clinical isolates from urinary tract infections in japan (2008). | in this study, the causative bacteria and their sensitivity to various antimicrobial agents as well as risk factors for quinolone-resistant escherichia coli were investigated in patients with acute uncomplicated cystitis or complicated cystitis by isolation and culture of bacteria from urine samples. in total, 1312 strains were isolated from 1009 patients with acute uncomplicated cystitis, including e. coli (63.3%), enterococcus faecalis (12.8%) and streptococcus agalactiae (4.6%). in addition, ... | 2011 | 21242062 |
| [splenic abscess secondary to a colosplenic fistula as the presenting manifestation of colon cancer]. | | 2010 | 21242902 |
| [research regarding extended-spectrum beta-lactamases produced by enterobacteria strains]. | to evaluate the susceptiblity of extended spectrum beta-lactamase (esbl) producing organisms among escherichia coli and klebsiella pneumoniae, isolated between january-october 2009 from hospital and community. | 2010 | 21243821 |
| in vitro and in vivo properties of bal30376, a β-lactam and dual beta-lactamase inhibitor combination with enhanced activity against gram-negative bacilli that express multiple β-lactamases. | bal30376 is a triple combination comprising a siderophore monobactam, bal19764; a novel bridged monobactam, bal29880, which specifically inhibits class c β-lactamases; and clavulanic acid, which inhibits many class a and some class d β-lactamases. the mic(90) was ≤ 4 μg/ml (expressed as the concentration of bal19764) for most species of the enterobacteriaceae family, including strains that produced metallo-β-lactamases and were resistant to all of the other β-lactams tested. the mic(90) for sten ... | 2011 | 21245441 |
| international transfer of ndm-1-producing klebsiella pneumoniae from iraq to france. | | 2011 | 21245442 |
| experimental phage therapy in treating klebsiella pneumoniae-mediated liver abscesses and bacteremia in mice. | intragastric inoculation of mice with klebsiella pneumoniae can cause liver abscesses, necrosis of liver tissues, and bacteremia. a newly isolated phage (φnk5) with lytic activity for k. pneumoniae was used to treat k. pneumoniae infection in an intragastric model. both intraperitoneal and intragastric administration of a single dose of φnk5 lower than 2 × 10(8) pfu at 30 min after k. pneumoniae infection was able to protect mice from death in a dose-dependent manner, but the efficacy achieved w ... | 2011 | 21245450 |
| intravenous colistin administration in neonates. | nosocomial infection due to multidrug-resistant gram-negative pathogens in intensive care units is a challenge for clinicians and microbiologists, and has led to resurgence of parenteral colistin use in the last decade. safety and efficacy data regarding intravenous colistin (colistimethate) use in neonates is sparse. we present our experience of efficacy and safety of colistimethate in the treatment of sepsis in critically sick term and preterm neonates. | 2011 | 21245777 |
| isolation of pathogenic aerobic bacteria from the blood of septicaemic neonatal calves and the susceptibility of isolates to various antibiotics. | an automated blood culture system (bactec 9240) was used for the isolation of aerobic bacteria from the blood of septicaemic neonatal calves. blood samples were collected from 90 clinically septicaemic and 20 healthy neonatal calves and inoculated into blood culture bottles. there were 89 significant isolates from 90 positive blood cultures using the bactec system. escherichia coli was the most common pathogen detected accounting for 56 (63%) out of 89 isolates. the other pathogens were beta-hae ... | 2010 | 21247017 |
| overexpression of sicam-1 in the alveolar epithelial space results in an exaggerated inflammatory response and early death in gram negative pneumonia. | a sizeable body of data demonstrates that membrane icam-1 (micam-1) plays a significant role in host defense in a site-specific fashion. on the pulmonary vascular endothelium, micam-1 is necessary for normal leukocyte recruitment during acute inflammation. on alveolar epithelial cells (aecs), we have shown previously that the presence of normal micam-1 is essential for optimal alveolar macrophage (am) function. we have also shown that icam-1 is present in the alveolar space as a soluble protein ... | 2011 | 21247482 |
| pulmonary nocardiosis caused by nocardia exalbida complicating pneumocystis pneumonia in an hiv-infected patient. | a 47-year-old man with optimally controlled type-2 diabetes mellitus and chronic hepatitis b was admitted to a local hospital because of a 1-week history of cough and high-grade fever. he was diagnosed with pneumocystis pneumonia (pcp) and klebsiella pneumonia from a chest radiograph and sputum. simultaneously, he was found to have hiv infection with a cd4 count of 76/μl. despite alteration of treatment secondary to the development of allergic reaction to trimethoprim-sulfamethoxazole (tmp-smx), ... | 2011 | 21249414 |
| enterobacterial microflora in infancy - a case study with enhanced enrichment. | to validate pmeu (portable microbe enrichment unit) method for monitoring the composition and development of infantile intestinal enterobacterial microflora. | 2011 | 21249478 |
| [antibiotic resistance of gram-negative bacillis isolated from children with bronchopneumonia]. | to study the distribution and antibiotic resistance of gram-negative pathogens isolated from children with bronchopneumonia. | 2011 | 21251380 |
| tigecycline activity tested against multidrug-resistant enterobacteriaceae and acinetobacter spp. isolated in us medical centers (2005-2009). | we evaluated the activity of tigecycline against enterobacteriaceae (9563 isolates) and acinetobacter spp. (835) with various resistance phenotypes collected from 31 us medical centers in 2005-2009. the isolates were tested for susceptibility by the reference broth microdilution method against tigecycline and various comparators. among escherichia coli and klebsiella spp., 6.8% and 15.4% exhibited an extended-spectrum β-lactamase (esbl) phenotype, respectively; and 22.2% of enterobacter spp. str ... | 2011 | 21251571 |
| emergence of kpc-producing klebsiella pneumoniae in texas. | | 2011 | 21251574 |
| new genetic environments of aac(6')-ib-cr gene in a multiresistant klebsiella oxytoca strain causing an outbreak in a pediatric intensive care unit. | | 2011 | 21251575 |
| detection of an ambler class d oxa-48-type β-lactamase in a klebsiella pneumoniae strain in the netherlands. | traditionally, bacteria in the netherlands have low levels of resistance to antibiotics. this report describes what is believed to be the first carbapenem-resistant klebsiella pneumoniae producing an oxa-48 type β-lactamase in the netherlands. the isolate co-produced a ctx-m-15 type β-lactamase and was recovered from a patient who was transferred from a hospital in india to an intensive care unit in the netherlands. his recovery in the netherlands was complicated by pneumonia due to the carbapen ... | 2011 | 21252273 |
| plasmid-borne 16s rrna methylase arma in aminoglycoside-resistant klebsiella pneumoniae in poland. | we characterized 17 clinical isolates of klebsiella pneumoniae producing 16s rrna methylase arma. the isolates originated in poland from 2002 to may 2010 and encompassed four xbai-pfge clusters. all the isolates were resistant to amikacin, gentamicin and kanamycin (mic range: 256-1024 mg l(-1)) and carried the arma gene on a large plasmid of approximately 90 or 130 kb in 15 and 2 isolates, respectively. the arma gene was found in a ~10 kb clai restriction fragment of the large plasmid and was fl ... | 2011 | 21252274 |
| epidemiology and risk factors for esbl-producing klebsiella pneumoniae: a case control study. | increased production of extended-spectrum β-lactamases (esbls) has become an important issue for treatment of severe klebsiella pneumoniae (k. pneumoniae) infections. this study aimed to evaluate risk factors of infection from esbl-producing k. pneumoniae (esbl-kp). | 2010 | 21252449 |
| plasmid-mediated quinolone resistance in expanded spectrum beta lactamase producing enterobacteriaceae in morocco. | although independently acquired, plasmid-mediated quinolone resistance appears to be linked with extended-spectrum or ampc-type beta-lactamases. since no data are available in african countries, the prevalence of qnr genes at the university hospital ibn rochd, casablanca, morocco, was investigated. | 2010 | 21252459 |
| molecular characterization and epidemiology of extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae isolates in the united arab emirates. | the aim of this prospective study was to assess the extent of dissemination of extended-spectrum ß-lactamase (esbl)-producing enterobacteriaceae at the hospital level in the united arab emirates (uae). | 2011 | 21252576 |
| comment on 'pink hypopyon caused by klebsiella pneumonia'. | | 2011 | 21252947 |
| identification of ctx-m15-, shv-28-producing klebsiella pneumoniae st15 as an epidemic clone in the copenhagen area using a semi-automated rep-pcr typing assay. | rapid molecular typing methods can be a valuable aid in the investigation of suspected outbreaks. we used a semi-automated repetitive sequence-based polymerase chain reaction (rep-pcr) typing assay and pulsed field gel electrophoresis (pfge) to investigate the relationship between local klebsiella pneumoniae (k. pneumoniae) producing extended spectrum ß-lactamases (esbls) and their relation to recognized danish outbreak strains. pfge and rep-pcr produced similar clustering among isolates. indivi ... | 2011 | 21253799 |
| site-directed alkylation studies with lacy provide evidence for the alternating access model of transport. | in total, 59 single cys-replacement mutants in helix vii and helix x of the lactose permease of escherichia coli were subjected to site-directed fluorescence labeling in right-side-out membrane vesicles to complete the testing of cys accessibility or reactivity. for both helices, accessibility/reactivity is relatively low at the level of the sugar-binding site where the helices are tightly packed. however, labeling of cys substitutions in helix vii with tetramethylrhodamine-5-maleimide decreases ... | 2011 | 21254783 |
| investigating the impact of the definition of previous antibiotic exposure related to isolation of extended spectrum β-lactamase-producing klebsiella pneumoniae. | previous antibiotic exposure is a risk factor for extended spectrum β-lactamase (esbl)-producing klebsiella pneumoniae isolation, but the optimal definition of previous antibiotic exposure remains unclear. | 2011 | 21255875 |
| multimerization-defective variants of dodecameric secretin puld. | the c-terminal core domain of the secretin puld from klebsiella oxytoca forms heat-resistant dodecameric complexes within less than 10min in an escherichia coli in vitro transcription-translation system containing liposomes, and is toxic when made in the cytoplasm without a signal peptide. random mutagenesis of dna encoding this region of puld revealed that amino acid changes throughout almost its entire length abolished toxicity. most of the amino acid substitutions engendered by the mutations ... | 2011 | 21256212 |
| sources of klebsiella and raoultella species on dairy farms: be careful where you walk. | klebsiella spp. are a common cause of mastitis, milk loss, and culling on dairy farms. control of klebsiella mastitis is largely based on prevention of exposure of the udder to the pathogen. to identify critical control points for mastitis prevention, potential klebsiella sources and transmission cycles in the farm environment were investigated, including oro-fecal transmission, transmission via the indoor environment, and transmission via the outdoor environment. a total of 305 samples was coll ... | 2011 | 21257074 |
| carbapenem resistance in klebsiella pneumoniae due to the new delhi metallo-β-lactamase. | carbapenem resistance in klebsiella pneumoniae is most notably due to the k. pneumoniae carbapenemase (kpc) β-lactamase. in this report, we describe the occurrence of a newly described mechanism of carbapenem resistance, the ndm-1 β-lactamase, in a patient who received medical attention (but was not hospitalized) in india. | 2011 | 21258100 |
| new delhi metallo-ß-lactamase and multidrug resistance: a global sos? | | 2011 | 21258101 |
| risk factors for the acquisition of nosocomial infection with carbapenem-resistant klebsiella pneumoniae. | carbapenem-resistant klebsiella pneumoniae (crkp) has been increasingly reported all over the world. in this study, we aimed to investigate the risk factors for the acquisition of nosocomial crkp infections. | 2011 | 21258230 |
| successful control of peristomal infection by introducer-type percutaneous endoscopic gastrostomy: a retrospective historical control study. | the techniques of percutaneous endoscopic gastrostomy are classified as the pull and introducer methods. peristomal infection is the most common procedure-related complication in the pull method. | 2011 | 21259072 |
| endogenous endophthalmitis associated with liver abscess caused by klebsiella pneumoniae. | to report two unusual cases of endogenous endophthalmitis associated with liver abscess caused by klebsiella pneumoniae. retrospective, interventional case series. two patients, known to have type ii diabetes mellitus, presented with sudden visual loss following several days of abdominal pain. examinations and investigations revealed endogenous endophthalmitis caused by k. pneumoniae. despite treatment in the form of intravitreal injection of antibiotics in the first patient and pars plana vitre ... | 2011 | 21264489 |
| klebsiella pneumoniae esbl producers responsible for severe utis in a renal transplant unit. | | 2011 | 21264678 |
| antimicrobial isopropenyl-dihydrofuranoisoflavones from crotalaria lachnophora. | two new isopropenyl-dihydrofuranoisoflavones exhibiting antimicrobial properties have been isolated along with eight known compounds from the cameroonian medicinal plant crotalaria lachnophora. the structures of the new compounds were elucidated by 1d and 2d nmr spectroscopy and high-resolution mass spectrometry as 7,2',4'-trihydroxy-5''-isopropenyl-4'',5''-dihydrofurano[2'',3'':5,6]isoflavone (1) and 4,8-dihydroxy-2-isopropenyl-2,3-dihydro-5h-[1]benzofuro[2,3-b]furo[3,2-g]chromen-5-one (2). the ... | 2011 | 21265557 |
| electron transfer and half-reactivity in nitrogenase. | nitrogenase is a globally important enzyme that catalyses the reduction of atmospheric dinitrogen into ammonia and is thus an important part of the nitrogen cycle. the nitrogenase enzyme is composed of a catalytic molybdenum-iron protein (mofe protein) and a protein containing an [fe4-s4] cluster (fe protein) that functions as a dedicated atp-dependent reductase. the current understanding of electron transfer between these two proteins is based on stopped-flow spectrophotometry, which has allowe ... | 2011 | 21265773 |
| is selective digestive decontamination useful in controlling aerobic gram-negative bacilli producing extended spectrum beta-lactamases? | to identify outbreak episodes of either carriage or infection due to extended spectrum beta-lactamases producing aerobic gram-negative bacilli (agnb-esbl); to establish whether agnb-esbl, sensitive to tobramycin, become resistant over time; and to evaluate the impact of selective decontamination of the digestive tract (sdd) on abnormal carriage of agnb-esbl. | 2011 | 21268690 |
| evaluation of the vitek 2 ast-n111 card for detection of extended-spectrum beta-lactamases (esbls) in escherichia coli, klebsiella pneumoniae, and klebsiella oxytoca compared to esbl etests and combination disk methods. | the vitek 2 ast-n111 card was evaluated for detection of extended-spectrum beta-lactamases (esbls) by testing 51 esbl positive and 50 esbl negative isolates of e. coli, k. pneumoniae, and k. oxytoca. the occurrence of beta-lactamase genes was confirmed by pcr and sequencing. the advanced expert system (aes) of the vitek 2 system achieved sensitivity and specificity values of 100% and 96.0%, respectively. the esbl test of the vitek 2 ast-n111 card showed a sensitivity of 92.1% and a specificity o ... | 2011 | 21271268 |
| a comparative study of induction of pneumonia in mice with planktonic and biofilm cells of klebsiella pneumoniae. | in the present study, the course of acute pneumonia in normal balb/c mice infected by intranasal inoculation of planktonic and preformed biofilm cells (3 days old) of klebsiella pneumoniae b5055 was studied and compared. with both cell forms the peak of infection was observed on the third post infection day, as assessed on the basis of lung bacterial load and corresponding pathology. there was an intense neutrophil infiltration in bronchoalveolar lavage fluid. tissue damage was assessed on the b ... | 2011 | 21272064 |
| infected cavitating pulmonary infarction. | | 2011 | 21276286 |
| development of a dna microarray for detection and identification of legionella pneumophila and ten other pathogens in drinking water. | the safety and accessibility of drinking water are major concerns throughout the world. consumption of water contaminated with infectious agents, toxic chemicals or radiological hazards represents a significant health risk and is strongly associated with mortality. therefore, we have developed an oligonucleotide-based microarray using the sequences of 16s-23s rdna internal transcribed spacer regions (its) and the gyrase subunit b gene (gyrb) found in the most prevalent and devastating waterborne ... | 2011 | 21276629 |
| cross-transmission of klebsiella pneumoniae in two intensive care units: intra- and inter-hospital spread. | | 2011 | 21277048 |
| [boerhaave's syndrome and pleural tuberculosis]. | boerhaave's syndrome is a rare condition involving spontaneous rupture of the oesophagus. the diagnosis is difficult and the prognosis depends on early diagnosis and treatment. | 2011 | 21277478 |
| klebsiella pneumoniae outer membrane protein a is required to prevent the activation of airway epithelial cells. | outer membrane protein a (ompa) is a class of proteins highly conserved among the enterobacteriaceae family and throughout evolution. klebsiella pneumoniae is a capsulated gram-negative pathogen. it is an important cause of community-acquired and nosocomial pneumonia. evidence indicates that k. pneumoniae infections are characterized by a lack of an early inflammatory response. data from our laboratory indicate that k. pneumoniae cps helps to suppress the host inflammatory response. however, it ... | 2011 | 21278256 |
| exudate macrophages attenuate lung injury by the release of il-1 receptor antagonist in gram-negative pneumonia. | exudate macrophages are key players in host defense toward invading pathogens. their antiinflammatory and epithelial-protective potential in gram-negative pneumonia, however, remains elusive. | 2011 | 21278303 |
| [detection of expanded-espectrum-betalactamases by diramic system: comparison with the double-disk synergy test and e-test]. | the capacity of the diramic system to detect strains producing extended-spectrum-betalactamase (esbl) was evaluated through the comparison with two phenotypic confirmatory tests: double-disk synergy test and e-test. ninety seven clinical isolates of escherichia coli y klebsiella spp. previously characterized; not repeated and suspected of being esbl producers were studied by the three methods. in comparison with e-test and double-disk synergy test, diramic system showed a sensitivity of 85.7% an ... | 2010 | 21279294 |