| activity of aminoglycosides, including achn-490, against carbapenem-resistant enterobacteriaceae isolates. | the emergence of carbapenemases in enterobacteriaceae is driving a search for therapeutic alternatives. we tested achn-490, a sisomicin derivative that evades all plasmid-mediated aminoglycoside-modifying enzymes, against 82 carbapenem-resistant enterobacteriaceae isolates. comparators included internationally and locally available aminoglycosides. methods the isolates variously had kpc (n = 12), sme-1 (n = 1), imp (n = 13), vim (n = 5), ndm (n = 17) or oxa-48 (n = 19) carbapenemases, or had com ... | 2010 | 21078604 |
| ct differentiation of pyogenic liver abscesses caused by klebsiella pneumoniae vs non-klebsiella pneumoniae. | objective: klebsiella pneumoniae is one of the organisms most commonly isolated from pyogenic liver abscesses in asian populations. we compared ct findings in liver abscesses caused by k. pneumoniae with those caused by other bacterial pathogens. methods: of 214 patients with liver abscesses examined over a 5 year period, 129 patients with positive blood or aspirate cultures were enrolled. the patients were divided into two groups: the k. pneumoniae monomicrobial liver abscess (kla) group (n = 5 ... | 2010 | 21081584 |
| exchange of peripherally inserted central catheters is associated with an increased risk for bloodstream infection. | it is not uncommon that the peripherally inserted central catheter (picc) needs to be replaced either due to blockage or migration to a peripheral position. in such circumstances, there are two methods of picc placement: new-site insertion and exchange by using the old picc as a guide wire. our objective was to investigate risk of infection associated with the exchange method. in this retrospective study, data on all picc insertions in the neonatal intensive care unit in 2004 to 2008 were obtain ... | 2010 | 21082537 |
| investigation of oral opportunistic pathogens in independent living elderly japanese. | gerodontology 2010; doi: 10.1111/j.1741-2358.2010.00449.x
investigation of oral opportunistic pathogens in independent living elderly japanese objective: pneumonia is reported to be associated with high morbidity in elderly and compromised individuals, with poor oral health demonstrated to be a significant risk factor for pneumonia. several opportunistic pathogens, such as streptococcus pneumoniae, klebsiella pneumoniae, escherichia coli and staphylococcus aureus, have been detected in patien ... | 2010 | 21083745 |
| heat resistance mediated by a new plasmid encoded clp atpase, clpk, as a possible novel mechanism for nosocomial persistence of klebsiella pneumoniae. | klebsiella pneumoniae is an important opportunistic pathogen and a frequent cause of nosocomial infections. we have characterized a k. pneumoniae strain responsible for a series of critical infections in an intensive care unit over a two-year period. the strain was found to be remarkably thermotolerant providing a conceivable explanation of its persistence in the hospital environment. this marked phenotype is mediated by a novel type of clp atpase, designated clpk. the clpk gene is encoded by a ... | 2010 | 21085699 |
| sepsis in premature newborns with congenital heart disease. | to determine the incidence and distribution of neonatal sepsis in premature newborns with congenital heart disease (chd) according to gestational and postnatal age, time of onset, and type and frequency of causing agents, and compare it with premature newborns without chd. | 2010 | 21087428 |
| antibiotic resistant mutants of escherichia coli k12 show increases in heterologous gene expression. | using a variety of antibiotics, it was found that nine separate isolates of spontaneous antibiotic resistant mutants of escherichia coli k12 ppsx-vioabcde overproduce the anti-tumour antibiotic violacein. subsequent analysis showed that seven of these mutations occurred on the plasmid ppsx-vioabcde. the other two overproducing strains carried spontaneous chromosomal mutations to lincomycin and kanamycin. the kanamycin resistant mutant of e. coli k12 dh10b (aa23) and a lincomycin resistant mutant ... | 2010 | 21087626 |
| study of nosocomial urinary tract infections in a pediatric intensive care unit. | nosocomial urinary tract infections (nuti) are one of the commonest infections in a pediatric intensive care unit (picu). this prospective study was conducted in picu between january and december 2008 to study the incidence, organisms and risk factors for nuti. a total of 287 consecutive patients with >48 h picu stay and sterile admission urine culture, were enrolled and monitored for nuti (defined as per cdc criteria 1988) till discharge or death. patients with and without nuti were compared wi ... | 2010 | 21088066 |
| virulence properties and integron-associated antibiotic resistance of klebsiella mobilis strains isolated from clinical specimens. | this study examined klebsiella mobilis isolates cultured from clinical specimens for virulence-associated properties and antibiotic resistance. the strains produced a number of siderophores, including enterobactin, aerobactin and yersiniabactin. all isolates were able to adhere to and invade epithelial cells. they had cytotoxic activity, which caused destruction of human laryngeal epithelial hep-2 cells and evoked lysis of murine macrophage j774 cells. analyses of hep-2 and j774 cellular morphol ... | 2010 | 21088128 |
| different clinical characteristics between polymicrobial and monomicrobial aeromonas bacteremia--a study of 216 cases. | part of aeromonas bacteremia is polymicrobial infection. however, a clinical comparison of monomicrobial and polymicrobial aeromonas bacteremia has not hitherto been reported. | 2010 | 21088342 |
| resistance status and evolution trends of klebsiella pneumoniae isolates in a university hospital in greece: ineffectiveness of carbapenems and increasing resistance to colistin. | due to its increased non-susceptibility rates, klebsiella pneumoniae has emerged as one of the most problematic pathogens. | 2010 | 21088396 |
| essential oil of thymus capitatus hoff. et link. from matmata, tunisia: gas chromatography-mass spectrometry analysis and antimicrobial and antioxidant activities. | thymus capitatus hoff. et link. essential oil was constituted by thymol (89.06%) as a major component followed by p-cimene (5.04%) and γ-terpinene (3.19%) after analysis by gas chromatography-flame ionization detection and gas chromatography-mass spectrometry. the antioxidant activity assays of the essential oil used in the inhibition of the radical cation 2,2'-azinobis(3-ethylbenzothiazoline-6-sulfonate) and the free radical 2,2-diphenyl-1-picrylhydrazyl showed high 50% inhibitory concentration ... | 2010 | 21091258 |
| first identification of a patient colonized with klebsiella pneumoniae carrying blandm-1 in taiwan. | new delhi metallo-β-lactamase 1 (ndm-1) is a novel type of metallo-β-lactamase (mbl). enterobacteriaceae carrying this ndm-1 encoding gene, bla(ndm-1), have been identified worldwide. bacteria carrying bla(ndm-1) are not only resistant to carbapenem, but also highly resistant to many classes of antibiotics, which indicate the importance of prompt identification of these bacteria and implementation of strict infection control measures to prevent their transmission. here, we report the first ident ... | 2010 | 21093828 |
| use of ecmo for resection of post-traumatic ruptured lung abscess with empyema. | we report a patient who sustained multiple injuries from a motorcycle collision. he had a protracted intensive care course complicated by ventilator-associated pneumonia and empyema resulting from a ruptured lung abscess. he successfully underwent a thoracotomy, lung resection, decortication, and pleurectomy on veno-venous extracorporeal membrane oxygenation. | 2010 | 21095362 |
| european guideline for the management of donovanosis, 2010. | donovanosis is a rare sexually transmitted infection now mainly seen in sporadic cases in papua new guinea, south africa, india, brazil and australia. the causative organism is calymmatobacterium granulomatis though a proposal has been put forward that the organism be reclassified as klebsiella granulomatis comb. nov. the incubation period is approximately 50 days with genital papules developing into ulcers that increase in size. four types of lesions are described - ulcerogranulomatous, hypertr ... | 2010 | 21097731 |
| ndm-1-producing klebsiella pneumoniae isolated in the sultanate of oman. | to analyse the mechanisms responsible for multidrug resistance in two carbapenem-resistant klebsiella pneumoniae isolates recovered from patients hospitalized in oman. | 2010 | 21098539 |
| a quarter of patients in some european hospitals acquire infections. | | 2010 | 21098589 |
| surveillance of antimicrobial susceptibility among bacterial isolates from intensive care unit patients of a tertiary-care university hospital in iran: 2006-2009. | this study was conducted to determine the antimicrobial susceptibility patterns among common pathogens in the intensive care unit (icu) of a university hospital in iran between 2006 and 2009. | 2010 | 21099220 |
| rare complications of pyogenic liver abscess. | pyogenic liver abscess (pla) is still associated with significant morbidity and mortality. with advances in imaging, most cases are now diagnosed early and effectively treated. however, complications, although considered rare, may still occur. we report three cases of pla that were associated with rare and significant complications. two patients had an abscess rupture that resulted in pyopericardium in one patient and sub-diaphragmatic abscess in the other. another patient with klebsiella pneumo ... | 2010 | 21103806 |
| [iatrogenic meningitis after diagnosis lumbar puncture: 3 cases reports in the paediatric children's hospital of tunis]. | we have collected cases of iatrogenic meningitis managed in the children's hospital of tunis, between january 1998 and december 2006. clinical information about each patient were collected, all bacterial samples were investigated in the microbiology laboratory of the hospital. bacterial isolates were identified according to conventional criteria. in the interval under study, we recorded three cases of iatrogenic meningitis after lumbar puncture. two cases occurred in newborn admitted for suspici ... | 2010 | 21103965 |
| [gram-negative bacteriae with resistance to carbapenems]. | clinically-significant gram-negative species remain mostly enterobacteriaceae, pseudomonas aeruginosa and acinetobacter baumannii. carbapenem molecules are often the last resort for treating infections due to multidrug resistant isolates. in enterobacteriaceae, resistance to carbapenems may result from combined mechanisms of resistance associating b-lactamases with weak (if any) intrinsic carbapenemase activity and decreased outer membrane permeability, or from true carbapenemases. kpc-type enzy ... | 2010 | 21106177 |
| pathogen-host interactions in dictyostelium, legionella, mycobacterium and other pathogens. | dictyostelium discoideum is a haploid social soil amoeba that is an established host model for several human pathogens. the research areas presently pursued include the use of d. discoideum to identify genetic host factors determining the outcome of infections and the use as screening system for identifying bacterial virulence factors. here we report about the legionella pneumophila directed phagosome biogenesis and the cell-to-cell spread of mycobacterium species. moreover, we highlight recent ... | 2010 | 21109012 |
| global spread of new delhi metallo-β-lactamase 1. | | 2010 | 21109168 |
| synthesis, antimicrobial and anticancer activity of new thiosemicarbazone derivatives. | thiosemicarbazones of p-aminobenzoic acid (paba) were synthesized and tested for their antimicrobial and anticancer activity. hydroxamate derivatives 4a-4l were found to have better antimicrobial and anticancer activity than their acid counterpart. compound 4d was found to have good antimicrobial activity against escherichia coli, klebsiella pneumoniae, staphylococcus aureus, vibrio cholerae, and bacillus subtilis with ic(50) value of about 1 µm. compound 4f showed potent antifungal activity aga ... | 2010 | 21110310 |
| [epidemiological and bacteriological characteristics of uropathogen bacteria isolated in a pediatric environment]. | urinary tract infection (uti) in children is a grave pathology, which requires a fast and effective care. bacteriological and epidemiological data play a determining role in patient's care. we report a retrospective study, which spreads out from january 1st till august 31st, 2009, having concerned hospitalized children for urinary infection in pediatrics service of farhat hached teaching hospital in sousse. our series contained 51 children with a sex ratio of 0.76, an average age of 32 months. t ... | 2010 | 21111582 |
| nmr structure and calcium-binding properties of the tellurite resistance protein terd from klebsiella pneumoniae. | the tellurium oxyanion teo(3)(2-) has been used in the treatment of infectious diseases caused by mycobacteria. however, many pathogenic bacteria show tellurite resistance. several tellurite resistance genes have been identified, and these genes mediate responses to diverse extracellular stimuli, but the mechanisms underlying their functions are unknown. to shed light on the function of kp-terd, a 20.5 -kda tellurite resistance protein from a plasmid of klebsiella pneumoniae, we have determined ... | 2010 | 21112337 |
| [occurrence of 16s rrna methylase arma producing enterobacteriaceae in a general hospital in warsaw, poland]. | resistance to gentamicin, amikacin and kanamycin was screened in 270 clinical isolates of enterobacteriaceae originated from april 19 to may 19, 2010 in a regular hospital in warsaw, poland. most of the isolated bacteria were considered pathogenic. nineteen isolates (7%) were simultaneously resistant to two or three of the tested aminoglycosides. mics of the three aminoglycosides ranged form 128 to 1024 mcg/ml for six isolates. these isolates were suspected to produce 16s rrna methylase. genes e ... | 2010 | 21114012 |
| resurgence of colistin: a review of resistance, toxicity, pharmacodynamics, and dosing. | colistin is a polymyxin antibiotic that was discovered in the late 1940s for the treatment of gram-negative infections. after several years of clinical use, its popularity diminished because of reports of significant nephrotoxicity and neurotoxicity. recently, the antibiotic has resurfaced as a last-line treatment option for multidrug-resistant organisms such as pseudomonas aeruginosa, acinetobacter baumannii, and klebsiella pneumoniae. the need for antibiotics with coverage of these gram-negati ... | 2010 | 21114395 |
| predation of human pathogens by the predatory bacteria micavibrio aeruginosavorus and bdellovibrio bacteriovorus. | the focus of this study was to evaluate the potential use of the predatory bacteria bdellovibrio bacteriovorus and micavibrio aeruginosavorus to control the pathogens associated with human infection. | 2010 | 21114596 |
| triggering receptor (trem-1) expressed on myeloid cells predicts bacteremia better than clinical variables in community-acquired pneumonia. | some clinical variables are associated with bacteremia in patients with community-acquired pneumonia (cap). the aim of this study was to analyse the accuracy of the soluble form of triggering receptor expressed on myeloid cells-1 (strem-1) to predict positive blood cultures in comparison with established clinical prognostic variables. | 2011 | 21114709 |
| modeling pilus structures from sparse data. | bacterial type ii secretion systems (t2ss) and type iv pili (t4p) biogenesis machineries share the ability to assemble thin filaments from pilin protein subunits in the plasma membrane. here we describe in detail the calculation strategy that served to determine a detailed atomic model of the t2ss pilus from klebsiella oxytoca (campos et al., pnas 2010). the strategy is based on molecular modeling with generalized distance restraints and experimental validation (salt bridge charge inversion; dou ... | 2010 | 21115127 |
| in-situ cr(vi) reduction with electrogenerated hydrogen peroxide driven by iron-reducing bacteria. | cr(vi) was reduced in-situ at a carbon felt cathode in an air-cathode dual-chamber microbial fuel cell (mfc). the reduction of cr(vi) was proven to be strongly associated with the electrogenerated h(2)o(2) at the cathode driven by iron-reducing bacteria. at ph 2.0, only 42.5% of cr(vi) was reduced after 12h in the nitrogen-bubbling-cathode mfc, while complete reduction of cr(vi) was achieved in 4h in the air-bubbling-cathode mfc in which the reduction of oxygen to h(2)o(2) was confirmed. conditi ... | 2010 | 21115243 |
| description of gibbsiella quercinecans gen. nov., sp. nov., associated with acute oak decline. | gram-negative, facultatively anaerobic bacterial strains were consistently isolated from oak trees displaying symptoms of extensive stem bleeding. in britain, this disorder is called acute oak decline (aod). a similar condition has been noted on species of mediterranean oak in spain. the identity of bacterial isolates from symptomatic trees in both countries was investigated using molecular techniques and phenotypic assays. 16s rrna gene sequencing indicated that the strains were most closely re ... | 2010 | 21115313 |
| ceftaroline activity against pathogens associated with complicated skin and skin structure infections: results from an international surveillance study. | to assess the spectrum and potency of ceftaroline, a novel anti-methicillin-resistant staphylococcal cephalosporin, against a 2008 surveillance collection of clinical isolates from patients in the usa and europe. | 2010 | 21115451 |
| detection of ndm-1-producing klebsiella pneumoniae in kenya. | seven carbapenem-resistant ndm-1-positive klebsiella pneumoniae isolates were recovered from patients hospitalized between 2007 and 2009 in different wards at a referral and tertiary care center in nairobi. most of the isolates were obtained from urine. all isolates carried the bla(ndm-1) carbapenemase gene previously reported from india, pakistan, and the united kingdom. these isolates were clonally related and expressed many other resistance determinants, including β-lactamases ctx-m-15, oxa-1 ... | 2010 | 21115785 |
| outbreak of colistin-resistant, carbapenem-resistant klebsiella pneumoniae in metropolitan detroit, michigan. | carbapenem-resistant klebsiella pneumoniae has spread worldwide and throughout the united states. colistin is used extensively to treat infections with this organism. we describe a cluster of colistin-resistant, carbapenem-resistant k. pneumoniae infection cases involving three institutions in detroit, mi. a cluster of five cases of colistin-resistant, carbapenem-resistant k. pneumoniae was identified at detroit medical center (dmc) from 27 july to 22 august 2009. epidemiologic data were collect ... | 2010 | 21115786 |
| practical considerations for building gromos-compatible small-molecule topologies. | molecular dynamics simulations are being applied to increasingly complex systems, including those involving small endogenous compounds and drug molecules. in order to obtain meaningful and accurate data from these simulations, high-quality topologies for small molecules must be generated in a manner that is consistent with the derivation of the force field applied to the system. often, force fields are designed for use with macromolecules such as proteins, making their transferability to other s ... | 2010 | 21117688 |
| [fatal pneumonia caused by carbapenem resistant klebsiella pneumoniae]. | a 63-year-old dutch man became colonized with a carbapenem resistant klebsiella pneumoniae during a period of hospitalization in india. his recovery in the netherlands was complicated by pneumonia due to this difficult-to-control multiresistant bacteria to which he eventually succumbed. carbapenem resistance in enterobacteriaceae, such as k. pneumoniae, is usually caused by carbapenemase (a betalactamase) production. carbapenemase producing enterobacteriaceae (cpe) are spreading throughout the w ... | 2010 | 21118587 |
| delayed infection after a zygoma fracture fixation with absorbable plates. | a 48-year-old man who had received a bioresorbable plate fixation for a zygomatic bone fracture 13 months earlier visited our clinic complaining of sudden facial swelling. the facial computed tomographic scan showed the soft tissue swelling without any bony abnormality, and the symptoms did not improve after 1 week of antibacterial therapy. the patient had a diagnosis of a late infection caused by unresorbed plates, and exploratory surgery was performed. partially resorbed plates and screws were ... | 2010 | 21119490 |
| emergence of klebsiella pneumoniae carbapenemase-producing bacteria. | klebsiella pneumoniae carbapenemase (kpc)-producing bacteria are a group of emerging highly drug-resistant gram-negative bacilli causing infections associated with significant morbidity and mortality. once confined to outbreaks in the northeastern united states (us), they have spread throughout the us and most of the world. kpcs are an important mechanism of resistance for an increasingly wide range of gram-negative bacteria and are no longer limited to k pneumoniae. kpc-producing bacteria are o ... | 2011 | 21119555 |
| a study of the prevalence of cytotoxic and non-cytotoxic klebsiella oxytoca fecal colonization in two patient populations. | klebsiella oxytoca is a cause of antibiotic-associated hemorrhagic colitis. few reports of the occurrence of k oxytoca within stool exist and there is no gold standard method for its isolation. | 2009 | 21119796 |
| [rapid infection of bone and soft tissues of the upper extremity--case report]. | long duration of intravenous drug abuse brings the risk of infectious and surgical complications. the case report of the forearm soft tissue and bone infection followed by rapid progress of compartment syndrome is described. primary bone infection is unusual. it is necessary to think about it if the treatment of soft tissues comes short of the inflammation suppression. especially if an intravenous drug abuser is concerned. the case stresses the necessity of thinking of all the diagnostic possibi ... | 2010 | 21121159 |
| susceptibility of klebsiella spp. to colistin and polymyxin b: results from the sentry antimicrobial surveillance program (2006-2009). | | 2010 | 21123034 |
| retrospective study of an outbreak in a kuwaiti hospital of multidrug-resistant klebsiella pneumoniae possessing the new shv-112 extended-spectrum beta-lactamase. | patients infected with bacteria producing extendedspectrum beta-lactamases (esbl) are at higher risk of mortality and morbidity. several mutations in genes encoding shv, tem and ctx-m beta-lactamases have been associated with esbl activity. this paper describes a new shv mutation in esbl-producing strains of klebsiella pneumoniae isolated in kuwait. the study included 13 k. penumoniae strains isolated from patients admitted to the amiri hospital of kuwait. the production of esbl in all strains w ... | 2010 | 21123157 |
| laboratory detection of extended-spectrum-beta-lactamase-producing enterobacteriaceae: evaluation of two screening agar plates and two confirmation techniques. | the worldwide prevalence of extended-spectrum-beta-lactamase-producing esbl-producing enterobacteriaceae (esbl-e) is increasing, making the need for optimized detection techniques more urgent. in this study we investigated the performance of two esbl-e screening and two esbl-e confirmation techniques. in accordance with the dutch national guidelines (www.wip.nl), a collection of 642 highly resistant enterobacteriaceae strains, as identified by vitek2, was used to test the performances of two scr ... | 2010 | 21123527 |
| multiplex real-time pcr assay for detection and classification of klebsiella pneumoniae carbapenemase gene (bla kpc) variants. | carbapenem resistance mediated by plasmid-borne klebsiella pneumoniae carbapenemases (kpc) is an emerging problem of significant clinical importance in gram-negative bacteria. multiple kpc gene variants (bla(kpc)) have been reported, with kpc-2 (bla(kpc-2)) and kpc-3 (bla(kpc-3)) associated with epidemic outbreaks in new york city and various international settings. here, we describe the development of a multiplex real-time pcr assay using molecular beacons (mb-pcr) for rapid and accurate identi ... | 2010 | 21123529 |
| purification and physiochemical characterization of melanin pigment from klebsiella sp. gsk. | the bacterium capable of producing melanin pigment in the presence of l-tyrosine was isolated from crop field soil sample and identified as klebsiella sp. gsk based on morphological, biochemical and 16s rdna sequencing. the polymerization of this pigment occurs outside the cell wall, which has granular structure as melanin ghosts. the chemical characterization of pigment particles showed acid resistant, alkali soluble, insoluble in most of the organic solvents and water. the pigment gets bleache ... | 2010 | 21124055 |
| homology between klebsiella pneumoniae and human aquaporin-4: no evidence for cross-reactivity in neuromyelitis optica. a study on 114 patients. | | 2010 | 21125292 |
| outbreak of nosocomial sepsis in the special care nursery at port moresby general hospital due to multiresistant klebsiella pneumoniae: high impact on mortality. | we report an outbreak of nosocomial infection caused by klebsiella pneumoniae in the special care nursery of port moresby general hospital. in the 13 months between october 2007 and october 2008, this organism was cultured from the blood of 57 neonates, of whom 23 died. 16 of the 20 organisms cultured in the first 3 months were cephalosporin sensitive, but during the next 10 months the proportion of sensitive organisms dropped dramatically to 10 of 37. of the 31 multidrug-resistant organisms 6 w ... | 2009 | 21125988 |
| phytochemical studies on stemona aphylla: isolation of a new stemofoline alkaloid and six new stemofurans. | a new stemofoline alkaloid, (2's)-hydroxy-(11s,12r)-dihydrostemofoline (3), new stemofurans m-r (8-13), and known compounds stemofoline (1), (2's)-hydroxystemofoline (2), stemofuran e (4), stemofuran f (5), stemofuran j (6), and stilbostemin f (7) have been isolated from the root extracts of stemona aphylla. the structures and relative configurations of these new compounds have been determined by spectroscopic data interpretation and from semisynthetic studies. these natural and semisynthetic al ... | 2010 | 21126060 |
| [comparison of the antimicrobial susceptibility testing with three automated systems for mrsa, visa, esbl-producing escherichia coli and klebsiella pneumoniae]. | some automated systems of the identification and susceptibility for microorganisms are used and prevail in hospital laboratories. one of the most serious problems is to perform accurate susceptibility testing for low-level resistant organisms, while antibiotic resistant microbes are increasing in medical fields. to evaluate automated machines for the susceptibility testing, several antibiotic resistant organisms were examined by plural technicians in some laboratories. each strain of methicillin ... | 2010 | 21128697 |
| pharmacokinetic evaluation of colistin sodium. | although colistin has recently played a key role in the treatment of nosocomial infections due to multidrug resistant gram-negative pathogens, there is a lack of clinical studies examining colistin pharmacokinetics (pks) in humans. this refers to all routes of colistin administration in clinical practice. colistin pk data are also limited in critically ill patients. | 2010 | 21128824 |
| ctx-m-15-producing escherichia coli clinical isolates in cairo (egypt), including isolates of clonal complex st10 and clones st131, st73, and st405 in both community and hospital settings. | in egypt, little is known about the genetic background of escherichia coli isolates harboring extended-spectrum β-lactamase (esbl). five hundred twenty enterobacteriaceae were prospectively collected (may 2007-august 2008) at the theodor bilharz research institute (cairo). among the collected enterobacteriaceae, 56% (n=291) were e. coli and 32% (n=165) klebsiella pneumoniae. a total of 16% (n=3) of all isolates were esbl, 19% (n=55) of the e. coli and 14% (n=23) of the k. pneumoniae. the proport ... | 2010 | 21128836 |
| comparison of (gtg)5-oligonucleotide and ribosomal intergenic transcribed spacer (its)-pcr for molecular typing of klebsiella isolates. | molecular typing of klebsiella species has become important for monitoring dissemination of β-lactamase-producers in hospital environments. the present study was designed to evaluate poly-trinucleotide (gtg)(5)- and rdna intergenic transcribed spacer (its)-pcr fingerprint analysis for typing of klebsiella pneumoniae and klebsiella oxytoca isolates. multiple displacement amplified dna derived from 19 k. pneumoniae (some with an esbl-phenotype), 35 k. oxytoca isolates, five k. pneumoniae, two k. o ... | 2010 | 21129413 |
| multidrug-resistant gram-negative bacteria: how to treat and for how long. | the emergence of multidrug-resistant (mdr) gram-negative bacilli creates a big problem for the treatment of nosocomial infections. as the pharmaceutical pipeline wanes, the only therapeutic options are two revived antibacterials (colistin and fosfomycin), a newer one (tigecycline) and an early-phase neoglycoside (achn-490). polymyxins, known since 1947, are mostly represented by polymyxin e (colistin), which has recently gained a principal position in the management of the most difficult-to-trea ... | 2010 | 21129924 |
| st11, the dominant clone of kpc-producing klebsiella pneumoniae in china. | klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae has spread rapidly in china. in this study, we aimed to investigate the molecular epidemiology of kpc-producing k. pneumoniae isolates in china. | 2010 | 21131324 |
| prevalence of transferable blactx-m-15 from hospital- and community-acquired klebsiella pneumoniae isolates in scotland. | this study was performed to investigate the prevalence and genetic characteristics of transferable bla(ctx-m-15) from hospital- and community-acquired klebsiella pneumoniae isolates in scotland. | 2010 | 21131694 |
| the effect of age on the bacteria isolated and the antibiotic-sensitivity pattern in infections among cancer patients. | patients with cancer are predisposed to infections. antimicrobial patterns and antibiotic sensitivity change with increasing age, making choice of empirical therapy more complicated. | 2010 | 21131751 |
| study on mechanisms of colonization of nitrogen-fixing pgpb, klebsiella pneumoniae ng14 on the root surface of rice and the formation of biofilm. | plant growth-promoting bacteria (pgpb) refer to the bacteria beneficial to plants, and they may affect the growth and development of plants directly or indirectly. this article studied the activities of nitrogen fixation and colonization of a strain of pgpb, klebsiella pneumoniae ng14, which was isolated from the rice root surface. the results showed that ng14 harbouring the nifh gene had nitrogenase activity, (15)n(2)-fixing activity, and was able to colonize on the root surface and within the ... | 2010 | 21132569 |
| 3-[benzimidazo- and 3-[benzothiadiazoleimidazo-(1,2-c)quinazolin-5-yl]-2h-chromene-2-ones as potent antimicrobial agents. | a series of 3-[benzimidazo(1,2-c)quinazolin-5-yl]-2h-chromene-2-one (6a-6f) and 3-[benzothiadiazole- imidazo(1,2-c)quinazolin-5-yl]-2h-chromene-2-one derivatives (7a-7f) that incorporate a variety of substituents at the 6- and/or 8-positions of the coumarin moieties have been synthesized utilizing cellulose sulfuric acid as an efficient catalyst under both conventional heating and microwave irradiation procedures. these analogs were evaluated for their antimicrobial activity against bacillus sub ... | 2010 | 21134751 |
| comparative effects of carbapenems on bacterial load and host immune response in a klebsiella pneumoniae murine pneumonia model. | doripenem is a carbapenem with potent broad-spectrum activity against gram-negative pathogens, including antibiotic-resistant enterobacteriaceae. as the incidence of extended-spectrum β-lactamase (esbl)-producing gram-negative bacilli is increasing, it was of interest to examine the in vivo comparative efficacy of doripenem, imipenem, and meropenem against a klebsiella pneumoniae isolate expressing the tem-26 esbl enzyme. in a murine lethal lower respiratory infection model, doripenem reduced th ... | 2010 | 21135187 |
| antimicrobial-resistant bacteria in a general intensive care unit in saudi arabia. | to assess the prevalence of multi-drug resistant (mdr) bacteria causing infections in patients at the intensive care units (icus) of riyadh military hospital (rmh), as well as their antimicrobial resistance patterns for one year. | 2010 | 21135998 |
| microbiological characteristics of catheter-related bacteremia in a tunisian intensive care unit. | prevalence of catheter-related bacteremia in intensive care units is increasing as central venous catheters (cvc) are used more frequently. in the most of the published literature, gram positive cocci are the leading cause of catheter-related bacteremia and the systemic empiric treatment recommended include the administration of glycopeptides. | 2010 | 21136352 |
| klebsiella pneumoniae pseudoaneurysm of the ascending aorta after coronary artery bypass graft. | mycotic pseudoaneurysm of the ascending aorta is rare in patients undergoing coronary artery bypass graft (cabg) and usually caused by staphylococcus aureus. we describe a patient with a mycotic pseudoaneurysm of the ascending aorta at the proximal vein graft anastomosis site after cabg. culture from the sinus tract of the sternum and from the aneurysm sac was klebsiella pneumoniae. surgical technique was patch repair of aorta under hypothermic circulatory arrest. he is asymptomatic at 24 months ... | 2010 | 21137660 |
| klebsiella-induced pneumothorax. | | 2010 | 21139308 |
| antimicrobial resistance in community-acquired urinary tract infections: results from the korean antimicrobial resistance monitoring system. | the korean antimicrobial resistance monitoring system was established and the first nationwide surveillance of bacterial uropathogens was conducted during the period from january 2008 to june 2009. with the cooperation of 34 medical centers throughout south korea, a total of 1994 strains belonging to clinically relevant bacterial uropathogens were collected from patients with community-acquired urinary tract infections (utis). to compare with past data, understand a trend of antimicrobial resist ... | 2010 | 21140281 |
| [genotypic analysis and plant growth-promoting ability of four plant growth-promoting bacteria from mangrove]. | we identified four strains of plant growth-promoting bacteria (pgpb) and their plant growth-promoting ability. | 2010 | 21141471 |
| extended measures for controlling an outbreak of vim-1 producing imipenem-resistant klebsiella pneumoniae in a liver transplant centre in france, 2003-2004. | we report the successful control of an outbreak caused by imipenem-resistant vim-1-producing klebsiella pneumoniae (ir-kp) in france. this outbreak occurred in a care centre for abdominal surgery that includes a 15-bed liver intensive care unit and performs more than 130 liver transplantations per year. the index case was a patient with acute liver failure transferred from a hospital in greece for urgent liver transplantation who was carrying ir-kp at admission as revealed by routine culture of ... | 2010 | 21144428 |
| carbapenem-non-susceptible enterobacteriaceae in europe: conclusions from a meeting of national experts. | the emergence and global spread of carbapenemase-producing enterobacteriaceae is of great concern to health services worldwide. these bacteria are often resistant to all beta-lactam antibiotics and frequently co-resistant to most other antibiotics, leaving very few treatment options. the epidemiology is compounded by the diversity of carbapenem-hydrolysing enzymes and the ability of their genes to spread between different bacterial species. difficulties are also encountered by laboratories when ... | 2010 | 21144429 |
| new delhi metallo-beta-lactamase 1-producing enterobacteriaceae: emergence and response in europe. | acquired carbapenemases confer extensive antibiotic resistance to enterobacteriaceae and represent a public health threat. a novel acquired carbapenemase, new delhi metallo-beta-lactamase 1 (ndm-1), has recently been described in the united kingdom and sweden, mostly in patients who had received care on the indian subcontinent. we conducted a survey among 29 european countries (the european union member states, iceland and norway) to gather information on the spread of ndm-1-producing enterobact ... | 2010 | 21144431 |
| possible importation and subsequent cross-transmission of oxa-48-producing klebsiella pneumoniae, france, 2010. | we report the possible first patient-to-patient transmission of klebsiella pneumoniae with decreased susceptibility to imipenem and producing oxa-48, ctx-m15, tem-1 and oxa-1 in a french hospital. | 2010 | 21144432 |
| control of a multi-hospital outbreak of kpc-producing klebsiella pneumoniae type 2 in france, september to october 2009. | an outbreak of klebsiella pneumoniae carbapenemase (kpc)-producing klebsiella pneumoniae type 2 was detected in september 2009 in two hospitals in a suburb south of paris, france. in total, 13 kpc-producing k. pneumoniae type 2 cases (four with infections and nine with digestive-tract colonisations) were identified, including a source case transferred from a greek hospital. of the 13 cases, seven were secondary cases associated with use of a contaminated duodenoscope used to examine the source c ... | 2010 | 21144448 |
| identification of a novel protein, prib, in klebsiella pneumoniae. | prib is a primosomal protein required for the reinitiation of replication in bacteria. here, we report the identification and characterization of a novel prib protein in klebsiella pneumoniae (kpn_04595; kpprib). unlike the well-studied escherichia coli prib protein (ecprib), which exists as a homodimer comprising 104-aa polypeptides, kpprib forms a monomer of only 55 aa, due to the absence of the 49 aa n-terminus in kpprib. although this n-terminal region (1-49 aa) in ecprib contains several im ... | 2010 | 21144832 |
| [epidemiological study of klebsiella spp. uropathogenic strains producing extended-spectrum β-lactamase in a tunisian university hospital, 2009.] | objective: an update on the prevalence of antibiotic resistance in extended-spectrum β-lactamase among urinary strains of klebsiella spp. isolated from in-come and out-come patients at university hospital, mahdia (tunisia). patients and methods: a retrospective survey was made over a period of twelve months (year 2009). it focused on 3564 patients with urinary tract infection confirmed by the laboratory of microbiology in the university hospital, mahdia. results: klebsiella spp. was involved in ... | 2010 | 21145175 |
| interspecies dissemination of the bla gene encoding per-1 extended-spectrum β-lactamase. | per-1 extended-spectrum β-lactamase-producing gram-negative bacilli are resistant to oxyimino-cephalosporins. however, the bla(per-1) gene has never been reported in klebsiella pneumoniae. here, we studied interspecies dissemination of the bla(per-1) gene by horizontal transfer of tn1213 among acinetobacter baumannii, pseudomonas aeruginosa, and k. pneumoniae. in a k. pneumoniae clinical isolate, the bla(per-1) gene was located on a 150-kbp incompatibility group a/c plasmid. | 2010 | 21149630 |
| do neutrophils play a role in establishing liver abscesses and distant metastases caused by klebsiella pneumoniae? | serotype k1 klebsiella pneumoniae is a major cause of liver abscesses and endophthalmitis. this study was designed to identify the role of neutrophils in the development of distant metastatic complications that were caused by serotype k1 k. pneumoniae. an in vitro cellular model was used to assess serum resistance and neutrophil-mediated killing. balb/c mice were injected with neutrophils containing phagocytosed k. pneumoniae. serotype k1 k. pneumoniae was significantly more resistant to serum k ... | 2010 | 21151499 |
| [comparison of four phenotypic methods to detect extended-spectrum betalactamases]. | to compare the efficacy of four phenotypic methods for the identification of strains producing extended-spectrum β-lactamases isolated from urine cultures. | 2010 | 21152726 |
| education and imaging: gastrointestinal: endoscopic unroofing of intramural gastric abscess with insulated tip knife. | | 2010 | 21155141 |
| [frequency of extended-spectrum β-lactamase (esbl) and ampc β-lactamase genes in escherichia coli and klebsiella pneumoniae over a three-year period in a university hospital in korea]. | the aim of this study was to determine the yearly prevalence and genotype distribution of extended-spectrum β-lactamase (esbl)-producing escherichia coli and klebsiella pneumoniae collected over a 3-yr period in gwangju, korea. | 2010 | 21157148 |
| ndm-1--a cause for worldwide concern. | | 2010 | 21158655 |
| [molecular typing of klebsiella pneumonia by pulse-field gel electrophoresis in combination with multilocus sequence typing.] | objective: to type klebsiella pneumonia through methods including pulse-field gel electrophoresis (pfge) in combination with multilocus sequence typing. methods: four selected different eps, referring to the standard operating procedure of pulsenet china, were used. the single colony of klebsiella pneumonia was quantified after enriched culture. embedding organisms in agarose and genome dna were lysed with proteinase k and then digested by restriction endonuclease xbai, to produce agarose gel. f ... | 2010 | 21162844 |
| [prevalence of plasmid-mediated ampc beta-lactamases in escherichia coli and klebsieila spp. isolated in children]. | to study the plasmid-mediated ampc β-lactamases and the genotypes of escherichia coli and klebsieila spp. in children. | 2010 | 21162906 |
| [study on pan-resistant klebsiella pneumoniae harboring bla(kpc-2) type carbapenemase gene from a hospital outbreak in huzhou, zhejiang.] | objective: to investigate the status of genotype of the kpc (klebsiella pneumoniae carbapenemase)-encoding genes in pan-resistant k. pneumoniae, isolated from the 98th hospital of people's liberation army, huzhou district, zhejiang province, china. methods: 19 strains of pan-resistant k. pneumoniae were isolated from the inpatients between november, 2008 and july, 2009. phenotypic confirmatory test for suspected carbapenemases production were carried out by modified hodge test. carbapenemase gen ... | 2010 | 21163037 |
| pharmacokinetics of polymyxin b in an infant with multidrug-resistant klebsiella pneumoniae bacteremia. | we report our experience with a 9-month-old infant treated with intravenous polymyxin b for multidrug-resistant klebsiella pneumoniae bacteremia. serial blood samples were obtained at steady state and serum drug concentrations were determined using a validated liquid chromatography-mass spectrometry method. the elimination half-lives of polymyxin b1 and isoleucine-polymyxin b1 were found to be 3.1 and 4.7 hours, respectively. | 2011 | 21164382 |
| neonatal sepsis in a tertiary care hospital in south india: bacteriological profile and antibiotic sensitivity pattern. | to identify the common bacterial pathogens associated with neonatal sepsis and their antibiotic susceptibility pattern. | 2010 | 21165715 |
| synthetic udp-furanoses as potent inhibitors of mycobacterial galactan biogenesis. | udp-galactofuranose (udp-galf) is a substrate for two types of enzymes, udp-galactopyranose mutase and galactofuranosyltransferases, which are present in many pathogenic organisms but absent from mammals. in particular, these enzymes are involved in the biosynthesis of cell wall galactan, a polymer essential for the survival of the causative agent of tuberculosis, mycobacterium tuberculosis. we describe here the synthesis of derivatives of udp-galf modified at c-5 and c-6 using a chemoenzymatic ... | 2010 | 21168771 |
| prognostic factors and outcomes in endogenous klebsiella pneumoniae endophthalmitis. | to determine risk factors for poor visual acuity outcomes in patients with endogenous klebsiella pneumoniae endophthalmitis. | 2010 | 21168820 |
| pharmacodynamic profiling of intravenous antibiotics against prevalent gram-negative organisms across the globe: the passport program-asia-pacific region. | due to escalating antimicrobial resistance amongst gram-negative organisms, the choice of effective empirical antimicrobial regimens has become challenging. monte carlo simulations were conducted for conventional and prolonged infusion regimens of doripenem, imipenem and meropenem using pharmacokinetic data from adult patients with conserved renal function. minimum inhibitory concentration data against escherichia coli, klebsiella pneumoniae, pseudomonas aeruginosa and acinetobacter baumannii we ... | 2010 | 21168997 |
| left ventricular assist device vegetation: "cure" without device explantation. | infection following the implantation of a left ventricular assist device (lvad) is a life-threatening complication with mortality rates ranging from 15% to 44%. staphylococcus aureus and staphylococcus epidermidis are the most frequently identified pathogens and are responsible for 60% of lvad-related infections, local as well as systemic. in this report we describe the successful therapeutic management of a patient who received a heart mate ii as "bridging-to-recovery", which was complicated by ... | 2010 | 21169190 |
| [risk factors for ventilator-associated pneumonia in neonates and the changes of isolated pathogens.] | objective: to study the risk factors for neonatal ventilator-associated pneumonia (vap) and the changes of isolated pathogens in the last eight years. methods: the clinical data of 230 neonates who were admitted into the neonatal intensive care unit (nicu) and received mechanical ventilation for equal to or longer than 48 hrs in 2008 were retrospectively reviewed. the isolated pathogens were compared with those of eight years ago. results: the incidence of vap (25.2%) in the year 2008 was lower ... | 2010 | 21172126 |
| first identified case of vim-producing carbapenem-resistant klebsiella pneumoniae in the republic of ireland associated with fatal outcome. | | 2010 | 21172170 |
| identification of ndm-1-producing enterobacteriaceae in norway. | | 2010 | 21172785 |
| use of a dictyostelium model for isolation of genetic loci associated with phagocytosis and virulence in klebsiella pneumoniae. | phagocytosis resistance is an important virulence factor in klebsiella pneumoniae. dictyostelium has been used to study the interaction between phagocytes and bacteria because of its similarity to mammalian macrophages. in this study, we used a dictyostelium model to investigate genes for resistance to phagocytosis in ntuh-k2044, a strain of k. pneumoniae causing pyogenic liver abscess that is highly resistant to phagocytosis. a total of 2,500 transposon mutants were screened by plaque assay, an ... | 2010 | 21173313 |
| the nationwide study of bacterial pathogens associated with urinary tract infections conducted by the japanese society of chemotherapy. | this study was conducted by the japanese society of chemotherapy and is the first nationwide study on bacterial pathogens isolated from patients with urinary tract infections at 28 hospitals throughout japan between january 2008 and june 2008. a total of 688 bacterial strains were isolated from adult patients with urinary tract infections. the strains investigated in this study are as follows: enterococcus faecalis (n = 140), escherichia coli (n = 255), klebsiella pneumoniae (n = 93), proteus mi ... | 2010 | 21174142 |
| gas in the renal shadow of a plain abdominal x-ray: emphysematous pyelonephritis. | | 2011 | 21175990 |
| clostridium difficile colonization in early infancy is accompanied by changes in intestinal microbiota composition. | clostridium difficile is a major enteric pathogen responsible for antibiotic-associated diarrhea. host susceptibility to c. difficile infections results partly from inability of the intestinal microbiota to resist c. difficile colonization. during early infancy, asymptomatic colonization by c. difficile is common and the intestinal microbiota shows low complexity. thus, we investigated the potential relationship between the microbiota composition and the implantation of c. difficile in infant gu ... | 2010 | 21177896 |
| dioxygenases in burkholderia ambifaria and yersinia pestis that hydroxylate the outer kdo unit of lipopolysaccharide. | several gram-negative pathogens, including yersinia pestis, burkholderia cepacia, and acinetobacter haemolyticus, synthesize an isosteric analog of 3-deoxy-d-manno-oct-2-ulosonic acid (kdo), known as d-glycero-d-talo-oct-2-ulosonic acid (ko), in which the axial hydrogen atom at the kdo 3-position is replaced with oh. here we report a unique kdo 3-hydroxylase (kdoo) from burkholderia ambifaria and yersinia pestis, encoded by the bamb_0774 (bakdoo) and the y1812 (ypkdoo) genes, respectively. when ... | 2010 | 21178073 |
| asymptomatic bacteriuria among hiv positive pregnant women. | the prognostic significance of asymptomatic bacteriuria resides in the observation that the prevalence is, relatively, high in persons with certain medical conditions, such as diabetes mellitus and pregnancy. this prevalence might, even, be higher in patients with human immunodeficiency virus infection. hence, this study set out to determine the prevalence of asymptomatic bacteriuria among symptom free and newly enrolled hiv infected pregnant women attending pmtct unit of antiretroviral clinic o ... | 2010 | 21178431 |
| comparing in vitro activity of tigecycline by using the disk diffusion test, the manual microdilution method, and the vitek 2 automated system. | tigecycline is a broad spectrum antibiotic having activity against multiresistant isolates. in vitro susceptibility testing is difficult to perform with the use of traditional microbiological techniques. the aim of this study was to evaluate the disk diffusion test with three different mueller-hinton agar brands, and the vitek 2 automated system in comparison with the standard broth microdilution method against 200 gram-negative isolates (escherichia coil, klebsiella pneumoniae, enterobacter clo ... | 2010 | 21180392 |
| the role of exopolysaccharides in dual species biofilm development. | a plasmid encoding the green fluorescent protein (gfp) of aequorea victoria was transformed into a biofilm-forming strain of enterobacter agglomerans originally isolated from an industrial environment. the transformed strain, entgfp, could then be identified in dual species biofilms by direct visualization, plate counts and quantitiative fluorescence measurements. a variety of cell constituents and products may be involved in the adhesion and accumulation process and exopolysaccharides (eps) rep ... | 1998 | 21182688 |