| carabapenem-resistant bacteria a looming threat in canada. | | 2010 | 20940242 |
| [the bacteriological profile of bacteriuria in residents of a long-term geriatric facility]. | little is known on the bacteriological profile of consecutive urine samples in elderly patients institutionalized in nursing homes. | 2010 | 20941920 |
| shv lactamase engineering database: a reconciliation tool for shv β-lactamases in public databases. | shv β-lactamases confer resistance to a broad range of antibiotics by accumulating mutations. the number of shv variants is steadily increasing. 117 shv variants have been assigned in the shv mutation table (http://www.lahey.org/studies/). besides, information about shv β-lactamases can be found in the rapidly growing ncbi protein database. the shv β-lactamase engineering database (shved) has been developed to collect the shv β-lactamase sequences from the ncbi protein database and the shv mutat ... | 2010 | 20942904 |
| the structure of kpn03535 (gi|152972051), a novel putative lipoprotein from klebsiella pneumoniae, reveals an ob-fold. | kpn03535 (gi|152972051) is a putative lipoprotein of unknown function that is secreted by klebsiella pneumoniae mgh 78578. the crystal structure reveals that despite a lack of any detectable sequence similarity to known structures, it is a novel variant of the ob-fold and structurally similar to the bacterial cpx-pathway protein nlpe, single-stranded dna-binding (ssb) proteins and toxins. k. pneumoniae mgh 78578 forms part of the normal human skin, mouth and gut flora and is an opportunistic pat ... | 2010 | 20944219 |
| management of infections due to kpc-producing klebsiella pneumoniae. | the emergence of the klebsiella pneumoniae carbapenemases in k. pneumoniae and other gram-negative bacteria, usually on a background of multidrug resistance, has led to difficult therapeutic choices. among available antibiotics, tigecycline and the polymyxins are the most frequently active against these organisms in vitro. optimal therapy of infections due to these bacteria may involve maximization of antibiotic dose as well as their use in combination. | 2009 | 20948700 |
| total synthesis and antimicrobial activity of a natural cycloheptapeptide of marine origin. | the present study deals with the first total synthesis of the proline-rich cyclopolypeptide stylisin 2 via a solution phase technique by coupling of the boc-l-pro-l-ile-l-pro-oh tripeptide unit with the l-phe-l-pro-l-pro-l-tyr-ome tetrapeptide unit, followed by cyclization of the resulting linear heptapeptide fragment. the chemical structure of the finally synthesized peptide was elucidated by ftir, ¹h/¹³c-nmr and fab ms spectral data, as well as elemental analyses. the newly synthesized peptide ... | 2010 | 20948913 |
| [time course of microbial colonization of different voice prostheses]. | for the vocal rehabilitation of laryngectomized patients, voice prostheses are actually one of the best known methods. caused by the surface colonisation with bacteria and fungi the life time of the prostheses is limited to 3-4 months. | 2010 | 20949410 |
| non-antibiotics reverse resistance of bacteria to antibiotics. | most clinical isolates that exhibit a multi-drug resistant phenotype owe that resistance to over-expressed efflux pumps. compounds that are efflux pump inhibitors (epis) reduce or reverse resistance to antibiotics to which the bacterial strain is initially resistant. we have evaluated non-antibiotics to reduce resistance of commonly encountered bacterial pathogens to antibiotics. | 2010 | 20952744 |
| antimicrobial susceptibility of intra-abdominal gram-negative bacilli from europe: smart europe 2008. | as part of the worldwide study for monitoring antimicrobial resistance trends (smart), a total of 3,030 clinical isolates of gram-negative bacilli from intra-abdominal infections were collected from 43 hospital centres from 13 european countries during 2008. of 51 species, the most commonly isolated species were escherichia coli (49.3%), followed by klebsiella pneumoniae (10.5%) and pseudomonas aeruginosa (8.6%). respectively, 17.9%, 11.6%, 5.5% and 4.5% of k. pneumoniae, e. coli, proteus mirabi ... | 2010 | 20953653 |
| aetiology and antimicrobial resistance of neonatal sepsis at a tertiary care centre in eastern india: a 3 year study. | to review aetiological agents of neonatal sepsis and their antibiotic resistance pattern over the past 3 years, at a 20 bedded level iii neonatal intensive care unit (nicu) in eastern india. | 2010 | 20953913 |
| solvent-free microwave extraction and hydrodistillation of essential oils from endemic origanum husnucanbaseri h. duman, aytac & a. duran: comparison of antibacterial activity and contents. | the essential oils (eos) obtained by solvent-free microwave extraction (sfme) and hydrodistillation (hd) from endemic origanum husnucanbaseri h. duman, aytac & a. duran were investigated using the gas chromatography-mass spectrometry system. the main constituents of both oils obtained from sfme and hd from o. husnucanbaseri are borneol (15.2-12.8%), α-terpineol (12.3-10.8%) and trans-sabinene hydrate (11.8-9.92%). the eo obtained from sfme contained substantially higher amounts of oxygenated com ... | 2010 | 20954094 |
| [incidence of surgical site infection after open heart surgery]. | despite modem surgical techniques, preoperative preventive use of antibiotics and optimal treatment of operative site, surgical site infections (ssi) are significant medical problem in the countries worldwide. | 2010 | 20954311 |
| [infected left atrial myxoma; report of a case]. | we report a rare case of infected left atrial myxoma. a 69-year-old male was admitted to our hospital due to cerebral infarction accompanied by lower limb ischemia. transesophageal echocardiography showed a mobile left atrial tumor. on the 16th hospital day, he sufferd from high fever and klebsiella pneumoniae was positive by blood culture. we excised the left atrial tumor, preventing systemic embolism and progression of sepsis. histological examination showed a typical myxoma and organized thro ... | 2010 | 20954360 |
| [progress on biodiesel production with enzymatic catalysis in china]. | this paper reports the progress of biodiesel production with enzymatic catalysis in beijing university of chemical technology, one of the leaders in biodiesel r & d in china, which includes screening of high-yield lipase production strains, optimization and scale-up of the lipase fermentation process, lipase immobilization, bioreactor development and scale-up, biodiesel separation and purification and the by-product glycerol utilization. firstly, lipase fermentation was carried out at industrial ... | 2010 | 20954390 |
| a carbapenem-resistant klebsiella pneumoniae isolate harboring kpc-1 from italy. | carbapenem-resistant gram-negative pathogens represent an emerging threat to the management of hospital-acquired infections. although the isolation of carbapenem-resistant enterobacteriaceae remains unusual, the frequency of carbapenemases producing klebsiella pneumoniae is increasing in different geographic regions: the majority of isolates has been collected in the usa, but recently kpc-producing k. pneumoniae were reported from china, israel, greece, france, norway and sweden. we report a kpc ... | 2010 | 20954450 |
| emergence of klebsiella pneumoniae coproducing kpc-2 and 16s rrna methylase arma in poland. | a klebsiella pneumoniae epidemic strain that coproduced carbapenemase kpc-2 (k. pneumoniae carbapenemase 2) and 16s rrna methylase arma has emerged in poland. four nonduplicate isolates from patients in a hospital in warsaw, poland, were found to carry the bla(kpc-2) and arma genes on ca. 50-kb and 90-kb plasmids, respectively. tn4401 with a 100-bp deletion in the variable region was detected in all the isolates. xbai pulsed-field gel electrophoresis (pfge) revealed 93.2% similarity of the isola ... | 2010 | 20956599 |
| microbes responsible for acute exacerbation of copd. | this study was designed to find out the microbes responsible for acute exacerbation of chronic obstructive pulmonary disease (copd). this study was carried out in the national institute of diseases of the chest & hospital (nidch), dhaka during the period of january 2003 to december 2003. the study was a prospective case control study. there were 88 male and 2 female patients. the majority of the study subjects fell within the range of 50-70 years. all were smokers. 30 stable copd patients were t ... | 2010 | 20956903 |
| microbial contamination of nonsterile pharmaceuticals in public hospital settings. | contamination of pharmaceuticals with microorganisms irrespective whether they are harmful or nonpathogenic can bring about changes in physicochemical characteristics of the medicines. although sterility is not a requirement in official compendia for nonsterile pharmaceuticals, bioburdens need to be within acceptable limits. therefore, this study investigated microbial contamination of 10 nonsterile pharmaceuticals frequently delivered to outpatients by identifying and quantifying microbial cont ... | 2010 | 20957135 |
| elimination of carbon catabolite repression in klebsiella oxytoca for efficient 2,3-butanediol production from glucose-xylose mixtures. | microbial preference for glucose implies incomplete and/or slow utilization of lignocellulose hydrolysates, which is caused by the regulatory mechanism named carbon catabolite repression (ccr). in this study, a 2,3-butanediol (2,3-bd) producing klebsiella oxytoca strain was engineered to eliminate glucose repression of xylose utilization. the crp(in) gene, encoding the mutant cyclic adenosine monophosphate (camp) receptor protein crp(in), which does not require camp for functioning, was characte ... | 2010 | 20957355 |
| involvement of apolipophorin iii in antibacterial defense of galleria mellonella larvae. | apolipophorin iii (apolp-iii) is an abundant hemolymph protein involved in lipid transport and immune response in insects. we investigated involvement of apolp-iii in the antibacterial response in galleria mellonella larvae. immune challenge with gram-negative (escherichia coli, klebsiella pneumoniae) and gram-positive (micrococcus luteus) bacteria led to an increase in the level of apolp-iii in g. mellonella hemolymph, 0.5-2h and 8h after treatment, respectively. apolp-iii purified from larval ... | 2010 | 20959145 |
| three enzymatic steps required for the galactosamine incorporation into core lipopolysaccharide. | the core lipopolysaccharides (lps) of proteus mirabilis as well as those of klebsiella pneumoniae and serratia marcescens are characterized by the presence of a hexosamine-galacturonic acid disaccharide (αhexn-(1,4)-αgala) attached by an α1,3 linkage to l-glycero-d-manno-heptopyranose ii (l-glycero-α-d-manno-heptosepyranose ii). in k. pneumoniae, s. marcescens, and some p. mirabilis strains, hexn is d-glucosamine, whereas in other p. mirabilis strains, it corresponds to d-galactosamine. previous ... | 2010 | 20959463 |
| bacterial community associated with the trunk latex of hancornia speciosa gomes (apocynaceae) grown in the northeast of brazil. | prevention or cure of different illnesses through the use of plant latex is a worldwide known concept. the antifungal activity of hancornia speciosa latex has been observed against candida albicans. however, h. speciosa latex is not a sterile plant exudate and secondary metabolites produced by bacteria could be involved in fungal inhibition. in the present study, the bacterial communities of the latex from three h. speciosa trees were characterized using traditional plating and molecular methods ... | 2010 | 20960060 |
| [successful isolation of imp-1 carbapenemase-producing, but not carbapenem-resistant species in enterobacteriaceae family]. | carbapenemases including klebsiella pneumoniae carbapenemases (kpc) are widespread among clinical isolates in the family enterobacteriaceae. in 2008, we isolated 4 imp-1 metallo-beta-lactamase (mbl) producers of this family having transferable carbapenem-resistance markers. when examined with microscan neg-combo panels, all 4 showed imipenem-mic of either <1 microg/ml or 2 microg/ml, although they were highly resistant to ceftazidime (mic: >16 microg/ml). when isolates were examined by sensi-dis ... | 2010 | 20960935 |
| prospective surveillance of nosocomial device-associated bacteremia in three adult intensive units in malaysia. | nosocomial blood stream infection (or nosocomial bacteremia) is a common problem in hospitals worldwide, including malaysia. a three-year prospective cohort study (october 2003-march 2007) of the incidences, risk factors, and patterns of the microorganisms causing bacteremia was conducted using a validated surveillance form in three intensive care units (icus) in malaysia. center for disease control criteria were used to diagnose bacteremia. patients were monitored from admission until the end p ... | 2010 | 20962730 |
| changing epidemiology of carbapenem-resistant enterobacteriaceae carriage during an outbreak of carbapenem-resistant klebsiella pneumoniae. | | 2010 | 20965609 |
| bacteriophage versus antimicrobial agents for the treatment of murine burn wound infection caused by klebsiella pneumoniae b5055. | this study was planned to evaluate the efficacy of silver nitrate and gentamicin in the treatment of burn wound infection and to compare it with phage therapy using an isolated and well-characterized klebsiella-specific phage, kpn5. a full-thickness burn wound was induced in mice and infected with klebsiella pneumoniae b5055 via the topical route. different concentrations of silver nitrate or gentamicin were applied topically daily after establishment of infection. phage kpn5 mixed in hydrogel w ... | 2010 | 20965914 |
| duplication of the chromosomal blashv-11 gene in a clinical hypermutable strain of klebsiella pneumoniae. | in a collection of 110 clinical isolates of klebsiella pneumoniae, a single strain, kp593, was found to exhibit a mutator phenotype with a rifampicin mutation frequency 100-fold higher than the modal value for this species. complementation experiments with the wild-type mutl, one of the main components of the methyl-directed mismatch repair system, allowed the mutator phenotype to be reversed. sequencing revealed substitution of the conserved residue lys307 to arg and site-directed mutagenesis f ... | 2010 | 20966089 |
| real-time polymerase chain reaction for rapid detection of genes encoding shv extended-spectrum β-lactamases. | this study aimed to develop an improved method for the detection of bacterial shv-type extended-spectrum β-lactamases (esbls). | 2010 | 20966564 |
| surveillance of device-associated infections at a teaching hospital in rural gujarat--india. | surveillance of hospital-acquired infection (hai), particularly device-associated infection (dai), helps in determining the infection rates, risk factors, and in planning the preventive strategies to ensure a quality healthcare in any hospital. the present study was carried out to know the prevalence of dai in a tertiary care teaching hospital of rural gujarat. | 2010 | 20966566 |
| comparison of isoelectric focusing and polymerase chain reaction for the detection of β-lactamases. | extended spectrum β-lactamases (esbls) have been observed in virtually all the species of family enterobacteriaceae. threat posed by antibiotic resistance because of esbls is more serious as a number of technical problems are associated with the detection of these enzymes. although a number of detection methods have been designed for esbls, every method has its own benefits and shortcomings as well. in earlier days, isoelectric focusing (ief) was used as the gold standard for esbl detection. thi ... | 2010 | 20966574 |
| nosocomial outbreak of septicaemia in neonatal intensive care unit due to extended spectrum β-lactamase producing klebsiella pneumoniae showing multiple mechanisms of drug resistance. | a total of 14 phenotypically similar clinical isolates of klebsiella pneumoniae, resistant to multiple drugs including cefotaxime and ceftazidime, were isolated from blood of neonates admitted to neonatal intensive care unit (nicu) within a short span of 10 days. alarmed at the possibility of occurrence of outbreak, a thorough investigation was done. microbiological sampling of the nicu and labour room (lr) environment yielded 12 k. pneumoniae isolates. the presence of extended spectrum β-lactam ... | 2010 | 20966575 |
| endogenous endophthalmitis: 10-year experience at a tertiary referral centre. | endogenous endophthalmitis (ee) is a sight-threatening emergency and the aetiology is often multifactorial. delayed diagnosis may exacerbate the poor visual prognosis. we describe the management and visual outcomes of ee presenting to a tertiary referral centre. | 2010 | 20966972 |
| vejovine, a new antibiotic from the scorpion venom of vaejovis mexicanus. | multidrug resistant bacterial infections are one of the most important health problems in recent years. resistance to conventional antibiotics limits the therapeutic options causing increase rate in morbid-mortality in hospitals. therefore, new antibacterial agents with new bacterial targets have been searched and found in many different sources, including scorpion venom and scorpion hemolymph. here, we report a new anti-microbial peptide named vejovine. this peptide was isolated from the venom ... | 2010 | 20969885 |
| klebsiella pneumoniae infection in solid organ transplant recipients: epidemiology and antibiotic resistance. | klebsiella pneumoniae is a well recognized source of nosocomial infection in solid-organ transplant (sot) recipients. it is also the most common species capable of producing extended-spectrum β-lactamases (esbl). its treatment can therefore be a challenge owing to antibiotic resistance. | 2010 | 20970577 |
| bloodstream infections caused by metallo-β-lactamase/klebsiella pneumoniae carbapenemase-producing k. pneumoniae among intensive care unit patients in greece: risk factors for infection and impact of type of resistance on outcomes. | to determine risk factors for bloodstream infections (bsis) caused by klebsiella pneumoniae producing metallo-β-lactamases (mbls) or k. pneumoniae carbapenemases (kpcs), as well as risk factors for mortality associated with carbapenem-resistant k. pneumoniae, among intensive care unit (icu) patients. | 2010 | 20973725 |
| aerosolized plus intravenous colistin versus intravenous colistin alone for the treatment of ventilator-associated pneumonia: a matched case-control study. | the incidence of ventilator-associated pneumonia (vap) due to multidrug-resistant (mdr) organisms is increasing. intravenous (iv) colistin or aerosolized (as) plus iv colistin have been recently used to treat these life-threatening infections. the purpose of this study was to compare the efficacy and safety of as plus iv colistin versus iv colistin alone for patients with mdr vap due to gram-negative bacteria. | 2010 | 20973727 |
| changing trends in newborn sepsis in sagamu, nigeria: bacterial aetiology, risk factors and antibiotic susceptibility. | sepsis is a major contributor to newborn deaths in the developing world. the objective is to determine the prevalence of newborn sepsis, the bacterial pathogens and antibiotic sensitivity pattern of the isolates. | 2010 | 20973858 |
| bacteremic community-acquired pneumonia due to klebsiella pneumoniae: clinical and microbiological characteristics in taiwan, 2001-2008. | klebsiella pneumoniae is the major cause of community-acquired pyogenic infections in taiwan. this retrospective study evaluated the clinical and microbiological characteristics of bacteremic community-acquired pneumonia due to k. pneumoniae in taiwanese adults. | 2010 | 20973971 |
| neutrophil elastase and myeloperoxidase regulate the formation of neutrophil extracellular traps. | neutrophils release decondensed chromatin termed neutrophil extracellular traps (nets) to trap and kill pathogens extracellularly. reactive oxygen species are required to initiate net formation but the downstream molecular mechanism is unknown. we show that upon activation, neutrophil elastase (ne) escapes from azurophilic granules and translocates to the nucleus, where it partially degrades specific histones, promoting chromatin decondensation. subsequently, myeloperoxidase synergizes with ne i ... | 2010 | 20974816 |
| dirty rotten scoundrels! | | 2010 | 20975459 |
| an outbreak of klebsiella pneumoniae and enterobacter aerogenes bacteremia after interventional pain management procedures, new york city, 2008. | in october 2008, an investigation was conducted into a cluster of gram-negative bloodstream infections after invasive pain management procedures at an outpatient facility to identify additional cases and determine the source of illness. | 2010 | 20975462 |
| liver injury and abscess formation in secondary murine peritonitis. | to investigate liver damage and abscess formation in murine, secondary peritonitis. | 2010 | 20976524 |
| [susceptibility surveillance of clinical isolates to fluoroquinolone antimicrobial agents from 2003 to 2008: post-marketing study of prulifloxacin]. | yearly changes in the susceptibility of clinical isolates to ulifloxacin (ufx) and other fluoroquinolones were examined through surveys over 3 periods. in the first survey, 534 strains derived from 19 species were collected from clinical specimens during 6 months from december 2003 to may 2004. in the same way, 805 strains were collected from december 2005 to may 2006 in the second survey, and 863 strains were from december 2007 to may 2008 in the third survey. over these 3 study periods, the su ... | 2010 | 20976880 |
| characteristics of klebsiella pneumoniae bacteremia in community-acquired and nosocomial infections in diabetic patients. | although diabetes mellitus is known as a major risk factor for klebsiella pneumoniae infection, the differences in clinical characteristics between community-acquired and nosocomial k. pneumoniae bacteremia in diabetic patients have been rarely reported. | 2010 | 20979704 |
| what are the risk factors for acute suppurative cholangitis caused by common bile duct stones? | acute suppurative cholangitis (asc), a severe form of acute cholangitis, is a life-threatening condition that must be treated with appropriate and timely management. the purpose of this study was to identify the factors that predispose patients to asc. | 2010 | 20981214 |
| klebsiella pneumonia treated with streptomycin. | | 1946 | 20987431 |
| klebsiella (friedländer's bacillus) infections in an army hospital. | | 1946 | 21019981 |
| ertapenem-resistant enterobacteriaceae: risk factors for acquisition and outcomes. | carbapenem resistance among enterobacteriaceae is of concern because of increasing prevalence and limited therapeutic options. limited research has been focused on understanding ertapenem resistance as a more sensitive marker for resistance to other carbapenems. we sought to determine risk factors for acquisition of ertapenem-resistant, meropenem-susceptible, or intermediate enterobacteriaceae and to assess associated patient outcomes. | 2010 | 21029005 |
| [carbapenem resistance in gram-negative bacteria]. | antibiotic resistance poses a serious threat to the successful treatment of hospitalized patients. micro-organisms that produce carbapenamases, such as klebsiella pneumoniae carbapenamases (kpcs) represent the next step in the continuously emerging problem of antibiotic resistance. restrictions on antibiotic use plus optimal adherence to infection control measures will be crucial to limit the spread of kpc in hospitals in the netherlands in the coming years. | 2010 | 21029499 |
| incidence of postoperative eye infections in a private eye hospital in lagos, nigeria. | there is a dearth of information on postoperative eye infections in nigeria and most hospitals do not have an infection control program in place. | 2010 | 21033323 |
| occurrence of gram-negative bacteria in hens' eggs depending on their source and storage conditions. | the aim of this study was to analyse the qualitative composition of gram-negative microbes, mainly of the family enterobacteriaceae, including pathogenic bacteria such as salmonella, in the albumens and yolks and on the shells of hens' eggs, depending on their source and on the temperature and duration of their storage. a total of 375 table eggs were studied, from a large-scale poultry farm, a small-scale poultry farm and a supermarket. each group was divided into 5 subgroups according to the te ... | 2010 | 21033566 |
| antibiotic susceptibility and genotype patterns of escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa isolated from urinary tract infected patients. | thirty nine isolates of escherichia coli, twenty two isolates of klebsiella pneumoniae and sixteen isolates of pseudomonas aeruginosa isolated from urinary tract infected patients were analyzed by antimicrobial susceptibility typing and random amplified polymorphic dna (rapd)-pcr. antibiotic susceptibility testing was carried out by microdilution and e test methods. from the antibiotic susceptibility, ten patterns were recorded (four for e. coli, three for k. pneumoniae and three for p. aerugino ... | 2010 | 21033585 |
| community-acquired klebsiella pneumoniae liver abscesses - an "emerging disease" in australia. | liver abscess due to klebsiella pneumoniae infection has been widely reported in asia, but rarely reported in australia until now. we describe four previously well asian-born patients who presented across australia with community-acquired k. pneumoniae liver abscesses. with prompt recognition, appropriate antibiotics and early drainage, outcome is significantly improved, although vigilance for metastatic complications is essential. | 2010 | 21034390 |
| emergence of klebsiella pneumoniae carbapenemase-producing proteus mirabilis in hangzhou, china. | carbapenems are used to treat severe infections caused by multi-drug-resistant organisms, however, the emergence of carbapenem-resistant bacterial isolates is becoming an increasing therapeutic challenge. since the first klebsiella (k.) pneumoniae carbapenemase (kpc)-producing k. pneumoniae was reported in 2001, kpc-producing isolates have been found increasingly, specially in enterobacteriaceae. the aim of this study was to characterize the mechanisms of a carbapenem-resistant proteus (p.) mira ... | 2010 | 21034629 |
| epidemiologic, clinical characteristics, and risk factors for adverse outcome in multiresistant gram-negative primary bacteremia of critically ill patients. | characteristics and burden of primary bacteremia because of multidrug-resistant (mdr) gram-negative bacteria (gnb) in intensive care unit (icu) patients remain understudied. | 2010 | 21035919 |
| phenotypic and genotypic characterization of β-lactam resistance in klebsiella pneumoniae isolated from swine. | little is known about the antimicrobial resistance mechanisms in klebsiella pneumoniae from swine in china. thus, this paper aims to demonstrate the β-lactam resistance phenotypes and genotypes of k. pneumoniae isolates from swine in southwestern china, detect possible new β-lactamase variants, and determine whether or not the variants differ in their antibiotic resistance. isolates from 58 unrelated diseased swine were collected from 61 pig farms in southwestern china from 2007 to 2009. among t ... | 2010 | 21035968 |
| surveillance of antimicrobial susceptibility of aerobic and facultative gram-negative bacilli isolated from patients with intra-abdominal infections in china: the 2002-2009 study for monitoring antimicrobial resistance trends (smart). | the objective of this study was to investigate the distribution and susceptibility of aerobic and facultative gram-negative bacilli (gnb) isolated from patients with intra-abdominal infections (iais) in china. from 2002 to 2009, minimum inhibitory concentrations of 14 antibiotics for 3420 aerobic and facultative gnb from up to eight hospitals in six cities were determined by the broth microdilution method. enterobacteriaceae comprised 82.9% (2834/3420) of the total isolates, with escherichia col ... | 2010 | 21036547 |
| improved 1,3-propanediol production with hemicellulosic hydrolysates (corn straw) as cosubstrate: impact of degradation products on klebsiella pneumoniae growth and 1,3-propanediol fermentation. | to improve 1,3-propanediol (1,3-pd) production by an economic and efficient approach, hemicellulosic hydrolysates (hh) used as cosubstrate resulted in more biomass and higher reducing power for 1,3-pd production. the effects of primary degradation products such as individual sugars (xylose, glucose, mannose, arabinose and galactose) and major inhibitors (furfural, acetate and formate) on the klebsiella pneumoiae growth and 1,3-pd production were investigated in this study. xylose and mannose cou ... | 2010 | 21036601 |
| trends in production of extended-spectrum beta-lactamases among enterobacteriaceae of clinical interest: results of a nationwide survey in belgian hospitals. | to assess the frequency and diversity of extended-spectrum β-lactamases (esbls) in enterobacteriaceae isolates in belgium. | 2010 | 21036771 |
| klebsiella brain abscess in a neonate. | klebsiella pneumoniae is rarely associated with neonatal cerebral abscess. a case of klebsiella brain abscess in a neonate is described. diagnosis of abscess was confirmed by magnetic resonance imaging and ultrasound-guided aspiration. | 2010 | 21037286 |
| [viable non-culturable bacteria]. | viable but non-culturable cells (vbnc) are defined as live bacteria, but which do not either grow or divide. such bacteria cannot be cultivated on conventional media (they do not form colonies on solid media, they do not change broth appearance), but their existence can be proved using other methods. the switch to the vbnc stage has been described and documented for several bacterial species: vibrio spp. (cholerae, vulnificus and other species), escherichia coli (including ehec), campylobacter j ... | 2010 | 21038700 |
| detection of bacteriuria and bacteremia in newborn calves by a catalase-based urine test. | bacteremia occurs frequently in newborn calves. the predictive value of clinical signs is low, suggesting the use of calf-side diagnostic tests. | 2010 | 21039861 |
| role of magnesium and a phagosomal p-type atpase in intracellular bacterial killing. | bacterial ingestion and killing by phagocytic cells are essential processes to protect the human body from infectious microorganisms. however, only few proteins implicated in intracellular bacterial killing have been identified to date. we used dictyostelium discoideum, a phagocytic bacterial predator, to study intracellular killing. in a random genetic screen we identified kil2, a type v p-atpase as an essential element for efficient intracellular killing of klebsiella pneumoniae bacteria. inte ... | 2010 | 21040356 |
| diol dehydratase-reactivating factor is a reactivase--evidence for multiple turnovers and subunit swapping with diol dehydratase. | adenosylcobalamin-dependent diol dehydratase (dd) undergoes suicide inactivation by glycerol, one of its physiological substrates, resulting in the irreversible cleavage of the coenzyme co-c bond. the damaged cofactor remains tightly bound to the active site. the dd-reactivating factor reactivates the inactivated holoenzyme in the presence of atp and mg(2+) by mediating the exchange of the tightly bound damaged cofactor for free intact coenzyme. in this study, we demonstrated that this reactivat ... | 2010 | 21040475 |
| activities of nxl104 combinations with ceftazidime and aztreonam against carbapenemase-producing enterobacteriaceae. | combinations of nxl104 with ceftazidime and aztreonam were tested against carbapenem-resistant members of the enterobacteriaceae. ceftazidime-nxl104 was active against strains with the oxa-48 enzyme or with combinations of impermeability and an extended-spectrum β-lactamase (esbl) or ampc enzyme and also against most klebsiella spp. with the kpc enzyme, but metallo-β-lactamase producers were resistant. aztreonam-nxl104 was active against all carbapenemase producers at 4 and 4 μg/ml, including th ... | 2010 | 21041502 |
| evaluation of ceftazidime and nxl104 in two murine models of infection due to kpc-producing klebsiella pneumoniae. | we evaluated the efficacy of nxl104, a novel β-lactamase inhibitor, in combination with ceftazidime (caz) in two murine infection models (septicemia and thigh infection). we chose two kpc-producing klebsiella pneumoniae strains (va-361 and va-406) showing mics of caz of ≥256 μg/ml. septicemia was induced by the intraperitoneal injection of kpc-producing k. pneumoniae followed 30 min later by a single subcutaneous treatment with caz alone or caz-nxl104 in ratios of 2:1, 4:1, 8:1, and 16:1. in thi ... | 2010 | 21041503 |
| novel insights into the mode of inhibition of class a shv-1 beta-lactamases revealed by boronic acid transition state inhibitors. | boronic acid transition state inhibitors (batsis) are potent class a and c β-lactamase inactivators and are of particular interest due to their reversible nature mimicking the transition state. here, we present structural and kinetic data describing the inhibition of the shv-1 β-lactamase, a clinically important enzyme found in klebsiella pneumoniae, by batsi compounds possessing the r1 side chains of ceftazidime and cefoperazone and designed variants of the latter, compounds 1 and 2. the ceftaz ... | 2010 | 21041505 |
| antibacterial prenylated acylphloroglucinols from the fern elaphoglossum yungense. | seven new prenylated acylphloroglucinols, yungensins a-g, were isolated from the diethyl ether extract of the scaly rhizomes and roots of an argentine collection of the fern elaphoglossum yungense. all the compounds contained a geranyl group attached to the filicinic acid-type residue. the diethyl ether extract and yungensins a-f displayed antibacterial effects against staphylococcus aureus and pseudomonas aeruginosa with mics ranging from 10 to 200 μg/ml. all tested acylphloroglucinols, except ... | 2010 | 21043474 |
| genetic characterization of esbl producing strains of klebsiella pneumoniae from tehran hospitals. | this study was conducted to determine the genetic characterization of extended-spectrum beta-lactamase (esbl) producing strains of klebsiella pneumoniae isolated from iranian patients in hospitals in tehran. | 2010 | 21045352 |
| evaluation of tigecycline activity in clinical isolates among indian medical centers. | resistance to multiple antibiotics among gram-positive cocci (gpc) and gram negative bacilli (gnb) is high in india. tigecycline, a glycylcycline antibiotic is a newer treatment option for emerging single or multidrug-resistant (mdr) gpc and gnb. | 2010 | 21045403 |
| renal stone culture: is it relevant? | | 2010 | 21045482 |
| a simple colorimetric method for testing antimicrobial susceptibility of biofilmed bacteria. | this study introduces a simple colorimetric method which can measure the antimicrobial susceptibility of bacteria in biofilms using trimethyl tetrazolium chloride (ttc) as an indicator of viable bacteria. the new method was utilized for the evaluation of antibiotic susceptibility of escherichia coli, klebsiella pneumoniae, and staphylococcus aureus biofilms. | 2010 | 21046353 |
| protective efficacy of dna vaccines encoding outer membrane protein a and ompk36 of klebsiella pneumoniae in mice. | the immunogenicity of dna vaccines expressing outer membrane proteins as antigens was evaluated in this study. dna vaccines consisting of vector pvax1 expressing either outer membrane protein a or ompk36 were injected into mice by either the intradermal or the intramuscular route. antibodies elicited were shown to be specifically reactive to ompa and ompk36 by immunoblotting. the immunoglobulin g (igg) antibodies elicited by both vaccines included igg1, igg2a, igg2b, and igg3. immunized mice exh ... | 2010 | 21048001 |
| [antimicrobial potential of metarhizium taii in vitro]. | to investigate the antimicrobial potential of metarhizium tail and its corresponding active fractions. | 2010 | 21049622 |
| phytochemical investigations of stemona curtisii and synthetic studies on stemocurtisine alkaloids. | the isolation of two new stemona alkaloids, 1-hydroxyprotostemonine and stemocurtisine n-oxide, and a new benzofuran, stemofuran l, from the root extracts of stemona curtisii is reported. the major known alkaloids from this plant extract, stemocurtisine, stemocurtisinol, and oxyprotostemonine, were also isolated along with oxystemokerrine n-oxide. the nonalkaloid components of this extract included a new benzofuran derivative, stemofuran l, the known stemofurans f, j, and k, dihydro-γ-tocopherol ... | 2010 | 21049906 |
| protective effects of antimicrobial peptide s-thanatin against endotoxic shock in mice introduced by lps. | sepsis continues to be a major unresolved medical challenge of the present. severe sepsis and septic shock are the leading causes of multiple organ failure and mortality in noncoronary intensive care units (icus). the primary reason of septic shock is the activation of host effecter cells by endotoxin and lipopolysaccharide (lps) associated with cell membranes of gram-negative bacteria. for these reasons, the key point of treatment is removing lps. s-thanatin (ts), an analog of thanatin, was syn ... | 2010 | 21050874 |
| in vitro assessment of the antimicrobial activity of new n-acyl-thiourea derivatives. | the qualitative screening of the susceptibility spectra of different microbial strains to the newly synthesized substances complexes was performed by adapted disk diffusion techniques, while the quantitative assay of the minimal inhibitory concentration (m.i.c., microg/cm3) value was based on liquid medium serial microdilutions. the compounds were solubilized in dimethylsulfoxide (dmso). the in vitro biological screening effects were tested against a microbial inoculum of approximately 1.5 x 10( ... | 2010 | 21053783 |
| identification of a globally distributed clinical streptomycin-resistance plasmid and other resistance determinants in a coastal bay of china. | to study streptomycin-resistant bacteria isolated from jiaozhou bay and their molecular determinants of resistance. | 2010 | 21054449 |
| [etiology and epidemic characteristics of hospital acquired pneumonia in children]. | to investigate the etiology and epidemic characteristics of hospital acquired pneumonia (hap) in children. | 2010 | 21055271 |
| [detection and drug resistance analysis of extended-spectrum beta-lactamases-producing shigella in pediatric patients]. | to investigate the occurrence and drug resistance of extended-spectrum beta-lactamases (esbls)-producing strains of shigella in pediatric patients, so as to provide information for clinical treatment. | 2010 | 21055309 |
| insights into membrane association of klebsiella pneumoniae nifl under nitrogen-fixing conditions from mutational analysis. | in klebsiella pneumoniae nitrogen fixation is tightly controlled in response to ammonium and molecular oxygen by the nifl/nifa regulatory system. under repressing conditions, nifl inhibits the nif-specific transcriptional activator nifa by direct protein-protein interaction, whereas under anaerobic and nitrogen-limited conditions sequestration of reduced nifl to the cytoplasmic membrane impairs inhibition of cytoplasmic nifa by nifl. we report here on a genetic screen to identify amino acids of ... | 2010 | 21057007 |
| sct in patients with carbapenem resistant klebsiella pneumoniae: a single center experience with oral gentamicin for the eradication of carrier state. | following an outbreak of carbapenem resistant klebsiella pneumoniae (crkp) bacteremia among inpatients in the hemato-oncology and bmt unit, we studied the course of this infection in patients undergoing intensive chemotherapy and sct. in addition, we conducted a pilot study aimed to eradicate crkp colonization in the gastrointestinal tract, using oral gentamicin. adult patients admitted to the bmt unit, identified as crkp carriers on surveillance rectal cultures, were included in the study. oral ... | 2010 | 21057549 |
| anti-inflammatory and enzyme inhibitory activities of a crude extract and a pterocarpan isolated from the aerial parts of vitex agnus-castus. | a new compound, 6a,11a-dihydro-6h-[1] benzofuro [3,2-c][1,3]dioxolo[4,5-g]chromen-9-ol was isolated from the ethyl acetate fraction of vitex agnus-castus. the structure of this compound was identified with the help of spectroscopic techniques ((13)c nmr, (1)h nmr, hmbc, hmqc, noesy and cosy). the compound showed low urease- (32.0%) and chymotrypsin- (31.4%) inhibitory activity, and moderate (41.3%) anti-inflammatory activity. the crude extract and various fractions obtained from the aerial parts ... | 2010 | 21058321 |
| antibacterial action of a heat-stable form of l-amino acid oxidase isolated from king cobra (ophiophagus hannah) venom. | the major l-amino acid oxidase (laao, ec 1.4.3.2) of king cobra (ophiophagus hannah) venom is known to be an unusual form of snake venom laao as it possesses unique structural features and unusual thermal stability. the antibacterial effects of king cobra venom laao were tested against several strains of clinical isolates including staphylococcus aureus, staphylococcus epidermidis, pseudomonas aeruginosa, klebsiella pneumoniae, and escherichia coli using broth microdilution assay. for comparison ... | 2010 | 21059402 |
| [recent progress in taxonomic studies on pathogenic nocardia and usefulness of the bacteria for the studies on secondary metabolites and antibiotic resistant mechanisms]. | the present taxonomic situation of pathogenic actinomycetes including nocardia was clarified, and the impact of genomic sequence information of nocardia farcinica ifm 10152 on taxonomic work is introduced. the number of cases of nocardiosis is on the rise along with the increasing number of immunocompromised patients in japan. from 1999 to 2007, 718 strains of pathogenic actinomycetes were received for identification by medical mycology research center (mmrc), chiba university. about 75% of thes ... | 2010 | 21060211 |
| melatonin inhibits microglial activation, reduces pro-inflammatory cytokine levels, and rescues hippocampal neurons of adult rats with acute klebsiella pneumoniae meningitis. | acute bacterial meningitis caused by klebsiella pneumoniae (k. pneumoniae) is a major health threat with a high mortality rate and severe neuro-cognitive sequelae. the intense pro-inflammatory cytokine released from calcium-mediated microglial activation plays an important role in eliciting neuronal damage in the hippocampal region. considering melatonin possesses anti-inflammatory and immuno-modulatory properties, the present study determined whether melatonin can effectively decrease inflammat ... | 2010 | 21062353 |
| a 6-year prospective surveillance study for healthcare associated infections in a neurology unit. | to assess the epidemiology of healthcare-associated infections (hais) in a neurology unit in a university hospital. | 2008 | 21063310 |
| the genus klebsiella. | | 1946 | 21064753 |
| pathogen-induced heart rate changes associated with cholinergic nervous system activation. | the autonomic nervous system plays a central role in regulation of host defense and in physiological responses to sepsis, including changes in heart rate and heart rate variability. the cholinergic anti-inflammatory response, whereby infection triggers vagal efferent signals that dampen production of proinflammatory cytokines, would be predicted to result in increased vagal signaling to the heart and increased heart rate variability. in fact, decreased heart rate variability is widely described ... | 2010 | 21068197 |
| molecular assessment of microbiota structure and dynamics along mixed olive oil and winery wastewaters biotreatment. | the major parcel of the degradation occurring along wastewater biotreatments is performed either by the native microbiota or by added microbial inocula. the main aim of this study was to apply two fingerprinting methods, temperature gradient gel electrophoresis (tgge) and length heterogeneity-pcr (lh-pcr) analysis of 16s rrna gene fragments, in order to assess the microbiota structure and dynamics during mixed olive oil and winery wastewaters aerobic biotreatment performed in a jet-loop reactor ... | 2010 | 21069557 |
| fur regulation of the capsular polysaccharide biosynthesis and iron-acquisition systems in klebsiella pneumoniae cg43. | the ferric uptake regulator fur has been reported to repress the expression of rmpa, a regulatory gene for the mucoid phenotype, leading to decreased capsular polysaccharide (cps) biosynthesis in klebsiella pneumoniae cg43. here, quantitative real-time pcr (qrt-pcr) analyses and electrophoretic mobility shift assays showed that fur also repressed the expression of the cps regulatory genes rmpa2 and rcsa. interestingly, deletion of rmpa or rcsa but not rmpa2 from the δfur strain was able to suppr ... | 2010 | 21071493 |
| [detection of norovirus rna in bivalve molluscs by using bacteria-culture-employed method (a3t method)]. | norovirus (nv) rna has rarely been detected in foods despite the use of highly sensitive methods such as rt-pcr and real-time rt-pcr. in the modified method (a3t method) reported previously, a bacterial culture process was introduced into the standard protocol for nv detection to remove some inhibitor(s) present in food ingredients. to confirm the efficiency of the a3t method and to examine nv contamination in bivalve molluscs, we tried to detect nv rna in bivalve molluscs on the market and in o ... | 2010 | 21071907 |
| post-surgical wound infections involving enterobacteriaceae with reduced susceptibility to β-lactams in two portuguese hospitals. | the post-surgical period is often critical for infection acquisition. the combination of patient injury and environmental exposure through breached skin add risk to pre-existing conditions such as drug or depressed immunity. several factors such as the period of hospital staying after surgery, base disease, age, immune system condition, hygiene policies, careless prophylactic drug administration and physical conditions of the healthcare centre may contribute to the acquisition of a nosocomial in ... | 2010 | 21073683 |
| orally administered colistin leads to colistin-resistant intestinal flora and fails to prevent faecal colonisation with extended-spectrum β-lactamase-producing enterobacteria in hospitalised newborns. | colonisation and infection with extended-spectrum β-lactamase-producing enterobacteriaceae (esbl-e) is an emerging problem. the aim of this study was to investigate whether colistin, which is reported to be effective against multiresistant enterobacteria, prevents esbl-e colonisation in neonates. for prophylaxis of necrotising enterocolitis, oral gentamicin (15 mg/kg/day) is routinely used in all neonates hospitalised at the neonatal intensive care unit of university hospital graz (austria). dur ... | 2010 | 21074372 |
| epidemiology of bacteraemia in hamad general hospital, qatar: a one year hospital-based study. | we conducted a one-year observational study from july 2007 to june 2008 to describe the epidemiology of bacteraemia at hamad general hospital. during this period, a total of 452 episodes of bacteraemia occurred, which corresponds to a rate of 19/1000 hospital admissions. most patients 58.8% (266/452) had community acquired bacteraemia, and primary bacteraemia accounted for 62.2% (281/452) of the cases. the most common source of bacteraemia was intravenous catheterization in 19.2% (87/452) but no ... | 2010 | 21074495 |
| high rates of quinolone resistance among urinary tract infections in the ed. | objectives: the objectives of this study are to examine antibiotic resistance rates and to determine appropriate empiric oral antibiotic for patients with urinary tract infections (utis) evaluated and discharged from the ed. methods: a retrospective, single-institution chart review study from august 2008 to march 2009 was conducted. adult patients seen in the ed with uti were identified for study inclusion from review of microbiology records. hospitalized or asymptomatic bacteriuria cases were e ... | 2010 | 21075586 |
| characterisation of source-separated household waste intended for composting. | large-scale composting of source-separated household waste has expanded in recent years in the nordic countries. one problem can be low ph at the start of the process. incoming biowaste at four composting plants was characterised chemically, physically and microbiologically. the ph of food waste ranged from 4.7 to 6.1 and organic acid concentration from 24 to 81 mmol kg(-1). the bacterial diversity in the waste samples was high, with all samples dominated by gammaproteobacteria, particularly pse ... | 2010 | 21075618 |
| long-term characteristics of healthcare-associated infections in a neonatal intensive care unit. | healthcare-associated infections in neonatal intensive care units (nicus) are associated with a significant risk of morbidity and mortality. knowledge regarding pathogens, primary sources of infection and antibiotic resistance in the nicu is essential for developing management strategies. this study aimed to analyze the long-term characteristics of healthcare-associated infections in a tertiary referral center in southern taiwan. | 2010 | 21075708 |
| bacterial growth kinetics in acd-a apheresis platelets: comparison of plasma and pas iii storage. | our objective was to determine the growth kinetics of bacteria in leukoreduced apheresis platelets (lr-ap) in a platelet (plt) additive solution (pas; intersol, fenwal, inc.) compared to lr-ap stored in plasma. | 2010 | 21077911 |
| susceptibility of community-acquired pathogens to antibiotics in africa and asia in neonates--an alarmingly short review. | to assess the susceptibility of community-acquired pathogens in neonatal sepsis to commonly prescribed antibiotics in sub-saharan africa and asia since 2002. | 2010 | 21078009 |