| reactivity of fungal organisms in tissue sections using anti-mycobacteria antibodies. | sixty-four cases of deep fungal infections diagnosed using pas or silver stains and 18 control cases of sarcoidosis, m. tuberculosis and m. leprae infection were stained using commercial polyclonal antibody raised against m. paratuberculosis (mp), m. duvalii (md), and bacillus calmette-guerin (bcg). nine of 13 cases of sporotrichosis stained positively using anti-mp antibody only; 13 of 14 cases of histoplasmosis stained with anti-bcg, anti-md, and anti-mp; seven cases of cryptococcosis had only ... | 1991 | 1918507 |
| [a case of human-immunodeficiency virus infection related mycobacterium tuberculosis with atypical clinical features]. | increasing rates of human immunodeficiency virus (hiv) related tuberculosis have been noted and recently the clinical importance of the disease has been mentioned. the diagnosis of tuberculosis is more difficult in those patients with hiv seropositive than those with seronegative, because those with seropositive have atypical clinical features. a 29-year-old male, who was infected with hiv heterosexually in central africa in 1986, was admitted to our hospital with a history of general malaise an ... | 1991 | 1919120 |
| mononuclear cell trafficking and plasma protein extravasation into the cns during chronic relapsing experimental allergic encephalomyelitis in biozzi ab/h mice. | cellular traffic and plasma protein extravasation across the blood-brain and blood-spinal cord barrier (bbb) have been studied during chronic relapsing experimental allergic encephalomyelitis in biozzi ab/h mice, using a simultaneous double radioisotope method. there was a general correlation between the clinical course of disease and bbb breakdown, including a resealing of the barrier during remission, although breakdown appeared slightly to precede clinical presentation. the brain was markedly ... | 1991 | 1919601 |
| metabolism of glycosaminoglycans in tissues of adjuvant arthritic rat. | the metabolic changes in the connective tissue glycosaminoglycans were studied in tissues of adjuvant induced arthritic rats. arthritic process was induced in rats with the inoculation of freund's adjuvant containing heat killed mycobacterium tuberculosis in paraffin oil. the connective tissue glycosaminoglycans were fractionated into sulfated and non-sulfated glycosaminoglycans by chemical and enzymatic methods. the biosynthesis of sulfated glycosaminoglycans was examined using radioactive labe ... | 1991 | 1922017 |
| a new hypothesis for the aetiology of crohn's disease--evidence from lipid metabolism and intestinal tuberculosis. | the stimulus for the immune response in crohn's disease is unknown. in each of 19 cases of crohn's disease evaluated by electron microscopy, epithelial cells of the ileum contained phagolysosomes with lamellar layers of lipid. these structures, now termed r or reactant bodies, are the proffered antigenic stimulus. they are proposed to be an amalgam of lipid (cholesterol esters, or phospholipids) and bacterial fragments (mycoplasma, mycobacteria or streptococci), which in combination are hypothes ... | 1991 | 1924055 |
| occult tuberculous postpneumonectomy space empyema four years after lung resection. | we describe a patient in whom a tuberculous postpneumonectomy empyema developed 4 years after resection for lung cancer. the clinical presentation was dominated by non-specific constitutional symptoms, without any chest complaints. a computed tomographic scan of the chest suggested inflammation in the postpneumonectomy space. ultimately mycobacterium tuberculosis was cultured from material aspirated by needle thoracocentesis. to our knowledge this is the first report of a tuberculous postpneumon ... | 1991 | 1924056 |
| [can we confirm that tuberculosis has been cured?]. | | 1991 | 1924234 |
| [bacteriostatic activity of blood in patients with pulmonary tuberculosis during polychemotherapy]. | polychemotherapy comprising 4-6 antituberculosis drugs and prescribed either only daily, daily or given on an intermittent basis (every other day) or only on an intermittent basis was given for 4-8 months to 102 patients with destructive pulmonary tuberculosis (in those newly diagnosed, treated earlier and with a process recurrence). four and, less frequently, three drugs were used per day. bacteriostatic blood activity (bba) was high in 90.2% of the patients, at the maximal concentrations, bact ... | 1991 | 1924237 |
| [immunoregulatory properties of monocytes/macrophages in patients with pulmonary tuberculosis]. | antigen-presenting ability of monocytes/macrophages (mc/mph) was studied in 26 patients with pulmonary tuberculosis and in 21 healthy donors. the above investigation has demonstrated that the ability of the mc/mph patients to present mycobacterial antigens was not only intact, but also appeared to be even higher than the reference values of mc/mph antigen presentation in healthy donors (0.7 and 0.28, respectively). the ability of mc/mph to secrete interleukin-1 (il-1) both spontaneously and as a ... | 1991 | 1924245 |
| primary tuberculosis of the parotid gland. | a very unusual presentation of mycobacterium tuberculosis in the parotid gland substance is described to suggest reexamination of the place of tuberculosis in the differential diagnosis of a parotid mass. in this patient, diagnosis was made postoperatively only by histologic examination of the excised specimen. when m. tuberculosis etiology is suspected, either clinically or at operation, culture confirmation should be tried, and a mantoux test should be performed to complete the investigation. | 1991 | 1924579 |
| empiric therapy for the immunocompromised host. | the use of empiric therapy for immunocompromised hosts has been one of the major advances in the management of such patients. such therapy has been put into practice primarily for patients with neutropenia induced by cytotoxic chemotherapy. the empiric antibiotic regimens include in their coverage the bowel, skin, and intravenous-catheter flora anticipated for patients in a particular hospital. less often, physicians treat empirically for opportunistic infections that complicate defects in helpe ... | 1991 | 1925322 |
| inactivation of mycobacterium tuberculosis and mycobacterium bovis by 14 hospital disinfectants. | epidemics of mycobacteria due to contamination of medical devices continue to occur. for this reason, we assessed the ability of disinfectants, generally used in hospitals for disinfecting noncritical and semicritical patient care items, to inactivate mycobacteria. a modified association of official analytical chemists' (aoac) tuberculocidal activity test, using middlebrook 7h9 broth as the primary subculture medium and neutralization by dilution, was used to assess the ability of 14 hospital di ... | 1991 | 1928175 |
| the impact of hiv on the usefulness of sputum smears for the diagnosis of tuberculosis. | in a developing country, 289 patients were examined for active pulmonary mycobacterial disease (sputum smear and culture) and hiv infection (serology) to compare the sensitivity and positive predictive value of sputum smears for diagnosing pulmonary tuberculosis in patients with and without antibodies to hiv. seventy-nine percent of hiv-seronegative vs 66% of hiv-seropositive patients with positive cultures for mycobacterium tuberculosis were smear positive (p less than .05), and a positive sput ... | 1991 | 1928536 |
| pectus excavatum and scoliosis. thoracic anomalies associated with pulmonary disease caused by mycobacterium avium complex. | we studied the frequency of pectus excavatum or otherwise abnormally narrowed anterior-posterior thoracic dimension and of thoracic scoliosis among consecutive series of 67 patients with pulmonary disease due to mycobacterium avium complex and 55 patients with pulmonary mycobacterium tuberculosis. among those with m. avium, pectus excavatum and abnormal narrowing was present in 27% and scoliosis was seen in 52%; overall, 47 of the 67 (70%) had one or both of these anomalies. by comparison, of th ... | 1991 | 1928970 |
| rapid serodiagnosis of human mycobacteriosis by elisa using cord factor (trehalose-6,6'-dimycolate) purified from mycobacterium tuberculosis as antigen. | igg antibodies against purified cord factor (trehalose-6,6'-dimycolate, tdm) in sera of 99 patients infected with mycobacteria (42 patients with tuberculosis excreting tubercle bacilli in the sputum, 11 patients with non-tuberculous mycobacteriosis excreting acid-fast bacilli in the sputum, and 46 patients without bacilli in the sputum but diagnosed as having pulmonary tuberculosis by chest x-ray films and physical examination), five patients with lung cancer, and 100 healthy controls which incl ... | 1991 | 1931132 |
| mycobacterium tuberculosis infection presenting as pancytopenia with hypocellular bone marrow. | | 1991 | 1938861 |
| amplification of a species-specific dna fragment of mycobacterium tuberculosis and its possible use in diagnosis. | in recent work, a species-specific mycobacterium tuberculosis dna fragment was cloned and sequenced. on the basis of its nucleotide sequence, two oligonucleotides were synthesized and used as primers for polymerase chain reaction (pcr) amplification. a 396-bp fragment was specifically amplified from the m. tuberculosis genome. no amplification was observed from any of 10 different mycobacterial strains, included those belonging to the m. tuberculosis complex. neither was this fragment amplified ... | 1991 | 1939567 |
| a systematic molecular analysis of the t cell-stimulating antigens from mycobacterium leprae with t cell clones of leprosy patients. identification of a novel m. leprae hsp 70 fragment by m. leprae-specific t cells. | both protective immunity and immunopathology induced by mycobacteria are dependent on ag-specific, cd4+ mhc class ii-restricted t lymphocytes. the identification of ag recognized by t cells is fundamental to the understanding of protective and pathologic immunity as well as to the design of effective immunoprophylaxis and immunotherapy strategies. although some t cell clones are known to respond to recombinant mycobacterial heat shock proteins (hsp) like hsp3 65, the specificity of most t cells ... | 1991 | 1940353 |
| tuberculous brain abscess. | | 1991 | 1940419 |
| antituberculosis agents. v: alpha-[5-(5-nitro-2-furyl)-1,3,4-oxadiazol-2-ylthio]acethydrazide and related compounds. | alpha-[5-(5-nitro-2-furyl)-1,3,4-oxadiazol-2-ylthio]aceth ydrazide, alpha-[5-(5-nitro-2-furyl)-1,3,4-oxadiazol-2-ylthio]acetamid e, delta-allyl-1-[( 5-(5-nitro-2-furyl)-1,3,4-oxadiazol-2-ylthio]acety) thiosemicarbazide, and other related compounds have been synthesised for testing against mycobacterium tuberculosis. | 1991 | 1941546 |
| [reactivities of various mycobacteria species against dna probes (gen-probe rapid diagnostic system) specific to mycobacterium tuberculosis complex, mycobacterium avium and mycobacterium intracellulare]. | various mycobacterial species (22 species, 178 strains) were studied for their reactivity to dna probe specific for mycobacterium tuberculosis complex (mtc), m. avium or m. intracellulare, using gen-probe rapid diagnostic system (gen-probe inc., san diego, calif., u.s.a.). all the mtc strains, including m. tuberculosis, m. africanum, m. bovis and m. microti reacted with mtc-dna probe at the % hybridization value of 42.8-51.9% (values higher than 10% are regarded as positive), but their reactivit ... | 1991 | 1942722 |
| [in vitro activities of newly developed quinolones, fleroxacin, lomefloxacin and sparfloxacin against mycobacterium tuberculosis]. | in vitro antituberculous activities of three newly developed quinolones, fleroxacin (flrx, am-833), lomefloxacin (lflx) and sparfloxacin (spfx, at-4140) were evaluated in comparison to that of ofloxacin (oflx) using m. tuberculosis strains isolated from patients and the ogawa egg medium. spfx was apparently more active than oflx, but both flrx and lflx were less active. spfx inhibited completely the growth of all 20 strains of m. tuberculosis isolated from patients who were not previously treate ... | 1991 | 1942726 |
| clinical features, diagnoses, and management of tuberculosis in immunocompromised hosts. | for many years tuberculosis has been known to occur with greater frequency among persons with disorders that impair host defenses. in most instances these processes interfere with the immune response to mycobacterium tuberculosis, whereas, in a few, such as silicosis, the probable abnormality is a nonimmune defect in macrophage function. infection with the human immunodeficiency virus (hiv) causes progressive and ultimately profound depression of both humoral and cell-mediated immunity and, thus ... | 1991 | 1942727 |
| [adoptive immunotherapy for pulmonary tuberculosis caused by multi-resistant bacteria using autologous peripheral blood leucocytes sensitized with killed mycobacterium tuberculosis bacteria]. | a patient with pulmonary tuberculosis caused by bacteria resistant to various anti-microbial agents was treated with adoptively transferred autologous peripheral blood leucocytes (pbl) sensitized with killed mycobacterium tuberculosis organisms in vitro. the 32-year-old man was admitted to our hospital from national sanitarium okinawa hospital with weight loss, high fever, and rapid aggravation on chest x-ray. patient's pbl obtained by leukapheresis and separated with ficoll-hypaque solution wer ... | 1991 | 1942728 |
| [tuberculosis and cystic fibrosis]. | mycobacterial infections are rarely reported in cystic fibrosis patients although they quite often develop predisposing risk factors such as underweight, secondary diabetes mellitus and chronic inflammatory pulmonary disease. furthermore glucocorticoid therapy is mandatory in some patients. cf heterozygotes are said to have a selective advantage due to an increased host resistance against mycobacterium tuberculosis. in this survey 1926 cf patients were investigated for the incidence of tuberculi ... | 1991 | 1942950 |
| [the spectrum of liver disease in infection by the human immunodeficiency virus: a study of 50 liver biopsies]. | the patients with human immunodeficiency virus (hiv) infection have hepatic abnormalities due to infective, neoplastic and toxic disorders. the aim of the present study was to describe the spectrum of liver disease in the hiv infection and to establish the usefulness and indications of hepatic biopsy. | 1991 | 1943276 |
| [mycobacterial infections: yield of bacillary microscopy in different clinical samples (1975-1988)]. | the yield of microscopy examination as a quick diagnostic test in several pulmonary and nonpulmonary samples referred to the mycobacterial laboratory of a general hospital is reviewed. | 1991 | 1943278 |
| [frequency of drug resistance on m. tuberculosis in hospitalized treated patients in east germany]. | the frequency of primary drug resistance in patients treated in hospital because of tuberculosis of the lung in 1988 was 1.4% (10 patients), there is a low decrease compared with years before. also the frequency of acquired drug resistance is with 14.9% lower compared to 1983 (20%). the resistance rate (acquired resistance) to rmp has been nearly the same since 1980, in about 3%. in patients from foreign countries the rate of resistance was higher than in native patients. in 18% resistance was f ... | 1991 | 1946266 |
| tuberculosis in patients infected with the human immunodeficiency virus. | a retrospective study of tuberculosis was undertaken among 125 patients infected with human immunodeficiency virus (hiv) who attended our regional infectious disease unit between 1986 and 1989. nine tb-positive patients (five english, three africans, one indian) were identified. in three patients who presented with pyrexia of unknown origin and no objective evidence of any organ involvement, the diagnosis of tb was established from examination of sputum induced by nebulized hypertonic saline. fo ... | 1991 | 1946941 |
| the use of the polymerase chain reaction test in the diagnosis of tuberculosis. | current techniques for laboratory diagnosis of tuberculosis have some serious limitations. these include the high cost and time required for the current assays. the development of a rapid, sensitive, specific and low-cost assay is therefore of considerable importance. we report here the development and laboratory testing of a polymerase chain reaction dna-based diagnostic test for the presence of mycobacterium tuberculosis in sputum. the assay shows a high level of sensitivity and specificity an ... | 1991 | 1948472 |
| efficacy of intermittent pyrazinamide in experimental murine tuberculosis. | cflp mice were infected intravenously with mycobacterium tuberculosis strain h37rv and the progress of chemotherapy was followed by counts of viable bacilli in the lung and spleen. after spleen counts had reached log10 7.0, 12 experimental groups, each containing 10 mice, were treated for 8 weeks with pyrazinamide (pza) given in mean daily dosages of 100, 200 or 400 mg/kg/day, with the interval between the doses within each dosage group being 1, 2, 4 or 8 days. all mice were also given 25 mg iso ... | 1991 | 1949213 |
| sustained release of isoniazid in vivo from a single implant of a biodegradable polymer. | in order to solve the problem of poor patient compliance, attempts were made to prolong the bioavailability of antimycobacterial drugs after a single administration. a single implant of polylactic-co-glycolic acid (plga) co-polymer containing isoniazid ensured its sustained release up to 6 weeks. the levels are comparable with those obtained from daily doses. homogenates of liver and lungs from animals killed at 6 weeks after a single implant showed high antimycobacterial activity against mycoba ... | 1991 | 1949214 |
| spectrum of immune reactivity to mycobacterial (bcg) antigens in healthy hospital contacts in south india. | in an effort to study the immunological responses to antigens of tubercle bacilli, 49 tuberculin positive and 41 tuberculin negative hospital contacts aged 20-29 years (staff nurses and students working in government rajaji hospital, madurai, south india) were studied for serum antibodies (igg, igm and iga classes) to bcg by elisa and diameter of induration to ppd by mantoux procedures. the two immunological parameters were correlated in regression analysis. the results have revealed higher anti ... | 1991 | 1949216 |
| detection of mycobacterium tuberculosis in sputum samples using a polymerase chain reaction. | a polymerase chain reaction (pcr) assay for the rapid detection of mycobacterium tuberculosis in sputum samples is described. the target dna is a 123-base pair (bp) segment of is6110, which is repeated in the m. tuberculosis chromosome and is specific for the m. tuberculosis complex. methodology used to lyse the mycobacteria, extract the dna, and amplify the 123-bp target dna is presented. the amplified pcr product is detected by examination of ethidium-bromide-stained acrylamide gels. an intern ... | 1991 | 1952448 |
| disseminated tuberculosis in the acquired immunodeficiency syndrome era. | to assess the influence of human immunodeficiency virus type 1 (hiv)-induced immunodeficiency on the clinical, radiographic, and pathologic features of disseminated tuberculosis (tb), we studied 79 patients presenting in 1984 through 1987 with miliary or focal disseminated disease due to mycobacterium tuberculosis, as well as 4 additional non-hiv patients diagnosed after 1987. clinically defined acquired immunodeficiency syndrome (aids) or aids-related complex (arc) was present in 51 (group 1). ... | 1991 | 1952449 |
| in vitro activity of the new difluorinated quinolone sparfloxacin (at-4140) against mycobacterium tuberculosis compared with activities of ofloxacin and ciprofloxacin. | mics of the new fluoroquinolone drugs ofloxacin, ciprofloxacin, and sparfloxacin (at-4140) for 10 strains of mycobacterium tuberculosis were determined by using both a bactec radiometric method and testing on solid 7h11 agar medium. radiometric mics by 7h12 broth testing ranged from 0.5 to 1.0, 0.25 to 0.5, and 0.1 to 0.2 microgram/ml for ofloxacin, ciprofloxacin, and sparfloxacin respectively, whereas mics in solid medium ranged from 0.5 to 1.0, 0.5 to 1.0, and 0.2 to 0.5 microgram/ml, respecti ... | 1991 | 1952872 |
| surgical management of resistant mycobacterial tuberculosis and other mycobacterial pulmonary infections. | between august 1983 and october 1990, 42 patients with resistant mycobacterium tuberculosis underwent 44 pulmonary resections. during the same time, 38 patients with mycobacterial infections other than tuberculosis had 41 pulmonary resections. all patients either were poor candidates for medical therapy alone or had existing complications requiring surgical intervention. there was one operative death in each group, both from adult respiratory distress syndrome (postpneumonectomy pulmonary edema) ... | 1991 | 1953131 |
| isoniazid resistant tuberculosis in a school outbreak: the protective effect of bcg. | an outbreak of isoniazid resistant tuberculosis occurred in a large second level school. a total of 1,160 teenage pupils were at risk. nineteen cases of tuberculosis were diagnosed, 15 were students, 9 of whom were among 251 non-vaccinated students and 6 among 909 vaccinated students. two cases of miliary tuberculosis, one of whom also had tuberculous (tb) meningitis, occurred in the non-vaccinated group. the number of children with heaf grade +3 or +4 was significantly greater among children wh ... | 1991 | 1954998 |
| concurrent bloodstream infection with histoplasma capsulatum and mycobacterium tuberculosis. | an immunosuppressed patient is the first to be reported with both histoplasmal fungemia and tuberculous bacteremia, emphasizing the need to consider polymicrobial infection in such patients and the utility of lysis-centrifugation and radiometric blood culture techniques. | 1991 | 1955956 |
| radiometric and conventional drug susceptibility testing of mycobacterium tuberculosis. | one hundred and four clinical isolates of m. tuberculosis were susceptibility tested by the radiometric method (rad) using the bactec system in parallel with a conventional modified proportion method (con). in the latter, the strains were tested against four concentrations of drugs in lowenstein-jensen medium (isoniazid (inh), streptomycin (sm) and ethambutol (emb)) or 7h10 agar medium (rifampicin (rif)) and reported as "sensitive", "intermediate" or "resistant" from the minimum inhibitory conce ... | 1991 | 1958354 |
| [antimycobacterial activities of rifamycin derivatives]. | the standard initial chemotherapy, chemotherapeutic activity of which depends mostly on the two potent bactericidal drugs, inh and rfp, has made a remarkable progress in the treatment of tuberculosis. however, certain difficult situations still remain in the treatment of resistant diseases, mostly in retreatment cases especially resistant to inh and/or rfp, and of the patients who are not able to continue the standard regimens because of side effects and/or severe complications with various orga ... | 1991 | 1960916 |
| [management of inh and rfp-resistant refractory tuberculosis]. | two issues are to be taken into consideration when we deal with the management of refractory tuberculosis in which bacilli are resistant to both inh and rfp, two major drugs in tuberculosis chemotherapy. one is how to treat such refractory tb cases and another issue is how to prevent the spread of such drug-resistant bacilli into the environment of the patients. in this symposium, therefore, they discussed on either of these two points. on the first issue of the problem, how to treat the refract ... | 1991 | 1960917 |
| rapid preliminary differentiation of species within the mycobacterium tuberculosis complex: proposition of a radiometric method. | a radiometric method using the "bactec 460-tb" apparatus now enables rapid obtaining of drug-susceptibility data and differentiation of clinical mycobacterial specimens into those belonging to the mycobacterium tuberculosis complex (comprised of m. tuberculosis, m. bovis, m. bovis bcg and m. africanum) and non-tuberculous mycobacteria, using a specific inhibitor (p-nitro-alpha-acetyl-amino-beta-hydroxypropiophenone). in the present work, further differentiation of the m. tuberculosis complex int ... | 1991 | 1961977 |
| splenic abscesses due to mycobacterium tuberculosis in patients with aids: is splenectomy necessary? | | 1991 | 1962084 |
| antituberculous immunity: new solutions to an old problem. | tuberculosis continues to be a serious public health problem worldwide. in europe and the united states, it is now primarily a disease of the elderly; the alcoholic; the drug abuser; central american, african, and asian immigrants; and patients with aids. new and improved antituberculous vaccines are urgently needed, as both prophylactic and therapeutic agents. recent advances in molecular biology, genetic engineering, and hybridoma technology make it possible to identify and clone the genes tho ... | 1991 | 1962110 |
| primary gastric tuberculosis presenting as pyloric outlet obstruction. | a 38-yr-old haitian male with a longstanding history of peptic ulcer disease presented with persistent vomiting and weight loss. barium studies and upper endoscopy revealed gastric outlet obstruction with prepyloric ulcerations. endoscopic biopsies were consistent with chronic gastritis. the patient did not respond to intensive medical management, including total parenteral nutrition, intravenous cimetidine, and nasogastric suctioning. at the time of surgery, large celiac lymph nodes were noted ... | 1991 | 1962630 |
| immunogenicity of mannophosphoinositides of mycobacteria: effect of cord factor (trehalose-6,6'-dimycolate). | attempts were made to enhance the immunogenicity of mannophosphoinositides of mycobacteria against experimental tuberculosis. it was found that immunization of mice with a combination of mannosides and cord factor, coupled to methylated bovine serum albumin (mbsa) could not further alter the protective efficacy of mannoside-mbsa complexes against challenge with a ld50 dose of m. tuberculosis h37rv as seen by survival-to-mortality rate, root-specific lung mass, lung density and bacterial counts r ... | 1990 | 1963168 |
| influence of immobilization on properties of polyphosphate glucokinase. | polyphosphate glucokinase from mycobacterium tuberculosis h37ra was covalently bound to the glutaraldehyde-activated collagen coating silica gel, to the nylon, and to the cnbr-activated sepharose. after immobilization kinetic properties of the enzyme were altered. | 1990 | 1965082 |
| malnutrition-induced impairment of resistance against experimental pulmonary tuberculosis. | | 1990 | 1967840 |
| identification of mycobacterium tuberculosis by polymerase chain reaction. | | 1990 | 1968160 |
| clinical and laboratory characteristics of hiv-1 infection in zimbabwe. | to define the impact of human immunodeficiency virus (hiv) infection in africa, clinical and laboratory investigations were conducted on 265 hiv-seropositive outpatients in zimbabwe. twenty-four of the study subjects were asymptomatic (asx), 124 had persistent generalized lymphadenopathy (pgl), and 117 had aids-related complex (arc). hiv infection was assessed by commercial elisa, western blots, synthetic peptide elisa, and measurement of p24 antigen. serum immunoglobulins, lymphocyte mitogen re ... | 1990 | 1972189 |
| sequence and structural homology between a mouse t-complex protein tcp-1 and the 'chaperonin' family of bacterial (groel, 60-65 kda heat shock antigen) and eukaryotic proteins. | a mammalian cytoplasmic protein tcp-1, encoded by a gene within the mouse t-complex, has been found to exhibit highly significant (p much less than 0.00001) sequence homology to the 'chaperonin' family of bacterial and eukaryotic proteins (viz. groel protein of e. coli, rubisco subunit binding protein of plant chloroplasts, yeast hsp58 and mammalian p1 proteins and 60-65 kda mycobacterial antigen). with the introduction of few gaps, the amino acid sequence of tcp-1 shows between 60-63% similarit ... | 1990 | 1972327 |
| [the effect of agonists and antagonists of histamine h1- and h2-receptors on the growth of mycobacterium tuberculosis h 37 ra]. | the influence of histamine h1- and h2-agonists and -antagonists on the growth of mycobacteria is described. while compounds which are strongly related to histamine improve the growth, predominantly h1-antagonists inhibit bacterial growth. | 1990 | 1974417 |
| heat-shock protein 65 as a beta cell antigen of insulin-dependent diabetes. | the primary beta-cell antigen of insulin-dependent diabetes is thought to be a protein with a molecular weight of approximately 64 kd. hyperthermic incubation and cytokines such as interleukin 1 beta, gamma interferon, and tumour necrosis factor induce synthesis of 64 kd protein by insulinoma cells. by western blot techniques, cross-reactivity was found between this 64 kd protein and monoclonal antibodies directed against mycobacterium tuberculosis heat-shock protein 65, but not with antibodies ... | 1990 | 1975377 |
| potential value of serology for diagnosis of extrapulmonary tuberculosis. | the possible benefits for improved diagnosis of extrapulmonary tuberculosis were evaluated by means of a modified serological competition assay. antibodies to the 38 kd mycobacterium tuberculosis antigen were detected in 73% of cases of extrapulmonary tuberculosis and 70% of smear-negative cases of pulmonary tuberculosis at a chosen specificity of 98%. of 55 patients (45%) whose diagnosis of tuberculosis was not made until 7 days or longer after admission, 76% were positive for the antibody. in ... | 1990 | 1975849 |
| hypothesis: is lung disease after silicate inhalation caused by oxidant generation? | inhaled silicate dusts may cause lung disease through their surface coordination of iron with subsequent oxidant generation via the fenton reaction. pneumoconiosis, irritant bronchitis, focal emphysema, and carcinoma may be produced by oxidants either directly through lipid peroxidation and protein inactivation, or indirectly by oxidant-mediated release of cytokines such as platelet-derived growth factor. the increased incidence of tuberculosis observed among silicate workers could be explained ... | 1990 | 1977006 |
| insertion element is986 from mycobacterium tuberculosis: a useful tool for diagnosis and epidemiology of tuberculosis. | is986 of mycobacterium tuberculosis belongs to the is3-like family of insertion sequences, and it has previously been shown to be present in multiple copies in the chromosome of m. tuberculosis. in this study we investigated the value of a is986-based dna probe in the diagnosis and epidemiology of tuberculosis. is986 was found only in species belonging to the m. tuberculosis complex. independent isolates of m. tuberculosis complex strains showed a very high degree of polymorphism of restriction ... | 1990 | 1977765 |
| high antigen reactivity in mononuclear cells from sites of chronic inflammation. | antigen-specific in-vitro responses of mononuclear cells from synovial fluid and peripheral blood of patients with rheumatoid arthritis were compared with those of mononuclear cells from pleural exudate and peripheral blood of non-rheumatoid-arthritis patients with chronic pleuritis not caused by tuberculosis. the antigens tested were an acetone-precipitable fraction of mycobacterium tuberculosis (ap-mt), an escherichia coli lysate containing the 65 kd heat-shock protein of m bovis bcg (65 kd/e ... | 1990 | 1978874 |
| lack of diagnostic value of guineapig test for tuberculosis. | | 1990 | 1978886 |
| perinatal prophylaxis of tuberculosis. | | 1990 | 1979096 |
| monoclonal elisa to detect active tuberculosis. | | 1990 | 1979131 |
| characterization of a mycobacterium tuberculosis insertion sequence belonging to the is3 family. | a repetitive element (is986), previously isolated from mycobacterium tuberculosis and shown to detect multiple restriction fragment-length polymorphisms (rflps), has been sequenced. it consists of a potential insertion sequence of 1358bp, with 30-bp inverted repeat ends. is986 has four potentially significant open reading frames (orfs): orfa1, orfa2 and orfb on one strand and orfc on the complementary strand. the sequences of the potential translated products identify is986 as a member of the is ... | 1990 | 1981088 |
| tuberculous tenosynovitis of the wrist. two case reports. | tuberculosis was first described in 1756 by acrel in a case report. subsequent reports of musculoskeletal tuberculosis documented the uncommon occurrence of hand and wrist involvement. the two cases presented here demonstrate the difficulty in eradicating the organism even with modern regimens of chemotherapy. intraoperative gram's stain and frozen sections were useful to rule out other etiologies. firm diagnosis must be established by tissue culture. the first case appeared cured after thorough ... | 1991 | 1984910 |
| [unilateral sacroiliitis in multiorgan tuberculosis]. | a tight but painless soft-tissue swelling in the left parasternal region, with chronic cough and lumbo-ischial pain becoming progressively more severe over several months, occurred in a 27-year-old man. the chest x-ray film was normal, radiography of the iliosacral joints revealed right-sided sacroiliitis. in the further course of the illness night sweat, nightly fever up to 38 degrees c and weight loss of 7 kg in two months, as well as severe treatment-resistant earache developed. when hospital ... | 1991 | 1985808 |
| effect of steroids on cerebrospinal fluid penetration of antituberculous drugs in tuberculous meningitis. | sixteen patients with oral isoniazid, pyrazinamide, rifampin, and intramuscular streptomycin for tuberculous meningitis were studied. the concentrations of isoniazid, pyrazinamide, rifampin, and streptomycin in cerebrospinal fluid (csf) obtained 3 hours after administration were 2.40, 34.78, 0.29, and 3.78 micrograms/ml, respectively. the csf concentrations of isoniazid and pyrazinamide were well above the minimum inhibitory concentration for mycobacterium tuberculosis. concentrations of rifampi ... | 1991 | 1988241 |
| a rapid method for identification of mycobacterium species by polyacrylamide gel electrophoresis of soluble cell proteins. | the sodium dodecyl sulphate-polyacrylamide gel electrophoresis (sds-page) profiles of an easily and rapidly prepared soluble protein fraction were used in conjunction with conventional techniques to identify different strains of mycobacterium tuberculosis, m. bovis, m. bovis bcg, m. africanum, m. avium, m. kansasii, m. marinum, m. gastri, m. simiae and m. malmoense. complete concordance of results from both methods was obtained with all species except those of the m. tuberculosis complex. with t ... | 1991 | 1990134 |
| return of tuberculosis: screening and preventive therapy. | approximately 25 percent of individuals exposed to mycobacterium tuberculosis become infected. of those, about 10 percent will develop clinically active tuberculosis at some time in their lives. the tuberculin skin test should be used to screen all patients, especially those at greatest risk of contracting the disease, such as the young and the old, and those with weakened immune systems from poor nutrition, alcohol and drug abuse, chronic illness and human immunodeficiency virus infection. depe ... | 1991 | 1990732 |
| an outbreak of tuberculosis in a shelter for homeless men. a description of its evolution and control. | an outbreak of tuberculosis at a shelter for homeless men was studied in detail to further the understanding of the epidemiology of tuberculosis in this setting. the shelter provides evening accommodations for men aged 50 yr and older. the capacity is approximately 200 clients, and the client pool is approximately 1,000 men/yr. during a 6-wk period in december 1986 and january 1987, seven cases of tuberculosis were diagnosed in shelter clients. nine cases were reported in clients during the prec ... | 1991 | 1990937 |
| no evidence for serological autoimmunity to islet cell heat shock proteins in insulin dependent diabetes. | recent studies in nonobese diabetic mice have implicated the autoimmune destruction of pancreatic islet cells with immunity to a beta cell protein cross-reactive to mycobacterium tuberculosis heat shock protein 65 (hsp 65). therefore, our studies examined serological immunity to islet cell hsp in humans with insulin-dependent diabetes (idd). heat shock of human islet cells in vitro markedly increased the synthesis of proteins of 72,000, 75,000, and 90,000 mr. no autoantibodies reactive to these ... | 1991 | 1991854 |
| antibody-mediated demyelination in experimental allergic encephalomyelitis is independent of complement membrane attack complex formation. | the effects of decomplementation by cobra venom factor (cvf) on the pathogenesis of inflammation and demyelination in experimental allergic encephalomyelitis (eae) and acute antibody-mediated demyelinating eae (adeae) have been quantified histologically and immunocytochemically. in rats immunized with 50 micrograms of myelin basic protein in freund's complete adjuvant containing 100 micrograms heat-killed mycobacterium tuberculosis h37ra, clinical signs of eae were completely suppressed by two i ... | 1991 | 1993358 |
| association between the 65-kilodalton heat shock protein, streptococcus sanguis, and the corresponding antibodies in behçet's syndrome. | the etiology of behcet's syndrome (bs) is unknown, but a number of streptococcal species have been implicated. a hypothesis was postulated that a shared antigen, such as a stress protein, might account for some of these findings. indeed, a rabbit antiserum against a 65-kda heat shock protein of mycobacterium tuberculosis revealed a corresponding 65-kda band with all six streptococcus sanguis strains examined and s. pyogenes but not with s. salivarius. by applying a panel of nine monoclonal antib ... | 1991 | 2004821 |
| mycobacterial disease associated with hiv infection. | mycobacterial disease is a major part of the spectrum of opportunistic infections (ois) associated with hiv infection. mycobacterium avium intracellulare (mai) and mycobacterium tuberculosis are the most common mycobacterial pathogens afflicting hiv-positive patients. infection with mai tends to be an oi of advanced aids, and the results of treatment are frequently unsatisfactory. m. tuberculosis tends to attack patients much earlier in the course of their hiv disease, responds to standard treat ... | 1991 | 2005473 |
| [the manifestations of extrapulmonary tuberculosis]. | seven weeks after a generalized cerebral seizure a 27-year-old woman from ghana developed nausea, vomiting and weight loss, gradually increasing over two weeks. cranial computed tomography revealed several hyperdense formations with extensive associated oedema and a midline shift. among extensive biochemical tests only a raised erythrocyte sedimentation rate of 24/50 mm and leukopenia of 2,600/microliters (with normal differential count) were notable. diagnostic laparotomy was performed because ... | 1991 | 2015778 |
| a 19-year follow-up of tuberculin reactors. assessment of skin test reactivity and in vitro lymphocyte responses. | tuberculin skin test reactivity decreases with time such that repeat ppd skin testing may result in reactions of less than 10 mm. this reactivity may be boosted in some individuals with a second tuberculin skin test. the immunologic basis of these observations remains unclear. we studied the relationship between skin-test reactivity and in vitro blastogenic response to ppd in a cohort of 22 individuals (aged 28 to 81 years) known to be tuberculin reactors (induration greater than or equal to 10 ... | 1991 | 2019174 |
| differential diagnosis of bacterial myositis in aids: evaluation with mr imaging. | magnetic resonance (mr) imaging was used to assess for the presence of bacterial myositis, rare outside the tropics, in 13 patients with either the acquired immunodeficiency syndrome (aids) (n = 11) or positive results of serologic tests for the human immunodeficiency virus but without other evidence of aids (n = 2). bacterial myositis was diagnosed in six patients: in five it was caused by pyogenic bacteria, and in the other, by mycobacterium tuberculosis; in each patient, little or no subcutan ... | 1991 | 2027969 |
| causes and clinical management of vertebral osteomyelitis in saskatchewan. | a retrospective review of all patients with vertebral osteomyelitis admitted to all saskatchewan referral hospitals from 1973 to 1986 was undertaken to determine the incidence and clinical characteristics of the disease. there were 73 patients, an incidence of 5.3 cases/million per year. erroneous initial diagnoses were common (41%). there was a significantly increased risk in patients older than 60 years. staphylococcus aureus was the most frequent organism. mycobacterium tuberculosis was prese ... | 1991 | 2028298 |
| [primary laryngeal tuberculosis caused by mycobacterium bovis]. | it is suggested that laryngeal tuberculosis is a common complication of pulmonary tuberculosis. the most frequent germ is mycobacterium tuberculosis. we present a case in which a infrequent bacillus, mycobacterium bovis, was isolated without lung afectation. considerations about morphologic and microbiologic findings are discussed. | 1991 | 2036266 |
| in vitro antimycobacterial activities of newly synthesized benzoxazinorifamycins. | newly synthesized rifamycin derivatives, krm-1648, krm-1657, krm-1668, krm-1686, and krm-1687, having the chemical structures of 3'-hydroxy-5'-(4-alkylpiperazinyl)-benzoxazinorifamycins (alkyl residues: isobutyl, propyl, sec-butyl, sec-butyl [r configuration], and sec-butyl [s configuration], respectively), were studied for their in vitro antimycobacterial activities. representative (krm-1648) mics for 90% of the strains tested, determined by the agar dilution method on 7h11 medium, of various p ... | 1991 | 2039206 |
| dexamethasone adjunctive treatment for tuberculous meningitis. | during a 5-year period, 280 of 2010 patients admitted to the meningitis ward of a referral hospital in cairo, egypt, were clinically diagnosed as having tuberculous meningitis and were treated with either antituberculous chemotherapy and dexamethasone or antituberculous chemotherapy alone. fatality rates and neurologic sequelae were compared for the 2 treatment groups in the 160 patients who had cerebrospinal fluid cultures positive for mycobacterium tuberculosis. the overall mortality rate of 5 ... | 1991 | 2041662 |
| rheumatic manifestations of neoplasia. | neoplasia in an important cause of rheumatic disease. the mechanisms are either direct infiltration of the bone and joint, or indirect infiltration with manifestations distant from the site of neoplastic involvement. many of the reports reviewed in this article center on direct associations. in particular, there is a report on polymyositis and dermatomyositis and the link with neoplasia. cases of reflex sympathetic dystrophy are also described in association with neoplasia. there is further disc ... | 1991 | 2043440 |
| tuberculosis of the common bile duct. | the intrahepatic biliary tree can occasionally be infected by mycobacterium tuberculosis, but tuberculosis of the common bile duct has not previously been reported. a 38-year-old man with obstructive jaundice, who was originally thought to have cholangiocarcinoma associated with opisthorchiasis (a common combination in thailand), was finally proved to have tuberculosis of the common bile duct with adjacent tuberculous lymphadenitis. following t-tube drainage and antitubercular therapy, he made a ... | 1991 | 2043518 |
| tuberculosis in children: a 13-year follow up of 1714 patients in a belgian home care centre. | from may 1970 to september 1983, 1714 children with different forms of primary tuberculosis were referred to the paediatric home care centre (enfants soignés au foyer, e.s.f.) of the brussels university hospital st.-pierre. they were subdivided in five groups: asymptomatic (33%), symptomatic (28%), dubious tuberculous infections (35%), high-risk contacts (3%) and unestablished diagnosis (1%). they were aged from 10 days to 19 years, and 82% of them were migrants of low socio-economic level. fift ... | 1991 | 2044605 |
| sequence and immunogenicity of the 70-kda heat shock protein of mycobacterium leprae. | the gene encoding the mycobacterium leprae 70-kda heat shock protein has been isolated from a cosmid library using a fragment of the clone jkl2. southern blot analysis of a positive clone identified a 4.4-kb fragment containing the entire coding region of the gene plus 2.4 kb upstream. sequencing revealed the gene to encode a 621-amino acid protein, bearing 56% identity with the escherichia coli dnak gene product and 47% and 46% identity with the human and caenorhabditis elegans hsp70, respectiv ... | 1991 | 2051024 |
| insertional mutagenesis and illegitimate recombination in mycobacteria. | mycobacteria, particularly mycobacterium tuberculosis, mycobacterium leprae, and mycobacterium avium, are major pathogens of man. although insertional mutagenesis has been an invaluable genetic tool for analyzing the mechanisms of microbial pathogenesis, it has not yet been possible to apply it to the mycobacteria. to overcome intrinsic difficulties in directly manipulating the genetics of slow-growing mycobacteria, including m. tuberculosis and bacille calmette-guérin (bcg) vaccine strains, we ... | 1991 | 2052623 |
| platelet-activating factor antagonists do not protect against the development of experimental autoimmune encephalomyelitis. | there is evidence suggesting the involvement of the platelet-activating factor (paf) in central nervous system (cns) functions. the possibility exists that paf may be relevant in eliciting cell-mediated autoimmune phenomena in cns. to assess the role of paf in the pathogenesis of experimental autoimmune encephalomyelitis (eae), male lewis rats were primed with whole spinal cord from guinea pig, emulsified in freund's adjuvant supplemented with 10 mg/ml of mycobacterium tuberculosis, h37ra strain ... | 1991 | 2056071 |
| chronic tuberculous empyema with bronchopleural fistula resulting in treatment failure and progressive drug resistance. | we treated five patients with a past history of tuberculous pleural infection that led to chronic, quiescent, loculated empyema. reactivation of tb was associated with formation of bpf and recovery of drug-susceptible mycobacterium tuberculosis from sputum. all patients had recurrence of positive sputum cultures that yielded tubercle bacilli resistant to drugs they were receiving. the lungs demonstrated gross thickening with calcification of both visceral and parietal pleura. two patients underw ... | 1991 | 2060330 |
| tuberculosis in a captive colony of pinnipeds. | tuberculosis was diagnosed in 10 of 16 otariid seals upon post mortem examination. the species involved were new zealand fur seals (arctocephalus forsteri), australian sea lions (neophoca cinerea) and an australian fur seal (arctocephalus pusillus doriferus). five seals died, four as a direct result of mycobacterial infection. one seal died of unrelated disease. the remaining 10 animals were subsequently tuberculin tested and then killed and necropsied. tuberculous lesions were seen in five. gro ... | 1991 | 2067051 |
| [the usefulness of pleural biopsy in benign or malignant pleurisy]. | retrospective studies of pleural biopsy, cytology and ada in pleural effusion were performed in 116 patients with pleural effusion between 1980 and 1988. pleural malignant disease was diagnosed in 25 patients (75.8%) by cytology, in 19 patients (57.6%) by pleural biopsy. thus, cytology should be performed first in patients with pleurisy. both of cytologic study and cea in pleural effusion were negative in 3 cases of squamous cell carcinoma. tuberculous pleuritis was diagnosed in 24 patients (50. ... | 1991 | 2067153 |
| diagnostic efficacy of pleural biopsy as compared with that of pleural fluid examination. | previous studies compared the diagnostic value of pleural fluid cytology versus pleural biopsy. no direct comparison of the procedures including data from a combination of all diagnostic tests performed on each specimen appears to have been done. a total of 385 patients with concurrent pleural biopsy and fluid examination were identified from 1973 to 1986. clinical records, histologic and cytologic preparations, and microbiologic and chemical data were reviewed. a total of 109 patients had a fin ... | 1991 | 2068057 |
| regulation of t-cell proliferative responses by cells from solid lung tissue of m. tuberculosis-infected mice. | we have studied proliferative responses to mycobacterial antigen preparation (ppd) and to non-specific stimuli of interstitial cells from the lungs of mycobacterium tuberculosis-infected cba mice. ppd-reactive lymphocytes appeared in the lung wall tissue in the course of chronic infection, but their proliferative capacity was totally inhibited by the lung macrophages. the latter were also able to suppress the proliferation of immune lymph node t cells. the mechanism of suppression clearly had tw ... | 1991 | 2071162 |
| [a case of miliary tuberculosis with cutaneous lesion]. | miliary tuberculosis associated with cutaneous lesion has been rarely reported. we report a case of miliary tuberculosis in whom the cutaneous lesion was confirmed as tuberculosis by skin biopsy and bacterial examination. a 46-year-old man was admitted because of cough, fever, sore throat and abnormal shadow on the chest x-ray. physical examination revealed an emaciated man with two ulcerous lesions overlying yellowed crust on the chest wall and fine crackles on the left side of the lung. labora ... | 1990 | 2077257 |
| [disseminated tuberculosis cutis colliquativa caused by hematogenous dissemination of mycobacterium tuberculosis]. | we report on a female patient suffering from tuberculosis cutis colliquativa. the skin lesions were caused by hematogenic dissemination of mycobacteria tuberculosis spreading from the affected urogenital tract. the diagnosis was confirmed by culturing. combined treatment with tuberculostatic agents for 5 months resulted in complete remission of the tuberculosis. | 1990 | 2080654 |
| intrathecal synthesis of soluble class i antigens (shla) in patients with hiv infection and tuberculous meningitis. | shla are soluble class i antigens produced by lymphocytes on early activation. we have studied the shla index ih = (csf shla/serum shla)/(csf albumin/serum albumin), which reflects the intrathecal synthesis (its) of shla in 23 intravenous drug abusers with central nervous system (cns) hiv infection. their mean ih value was increased and directly correlated with its of igg against hiv when the total group of patients was studied; however, 8 of them, who suffered from concomitant tuberculous menin ... | 1990 | 2089132 |
| cerebrospinal fluid indices in cryptococcal and tuberculous meningitis: the spider web coagulum and its diagnostic significance. | the differentiation between a chronic cryptococcal meningitis and a chronic tuberculous meningitis may cause problems for the clinician only if standard microbiological methods are not applied to the diagnosis of both infections. in a male non-aids patient (50 y), 11 years after a suggested diagnosis of "tuberculous meningitis", meningoencephalitis with hydrocephalus was diagnosed and treated accordingly without success. mycobacterium tuberculosis was never found. because fibrin fibres of a spid ... | 1990 | 2090936 |
| evolving concepts of the epidemiology, diagnosis, and therapy of mycobacterium tuberculosis infection. | tuberculosis in the united states is evolving in nearly all respects--epidemiology, diagnosis, treatment, and prophylaxis. today a relatively larger segment of the population has predisposing factors to infection with tuberculosis. there is a greater percentage of people who are elderly, who have immigrated from countries endemic for tuberculosis, or who are immunosuppressed due to medications necessary for other conditions, because of malignancies, or because of infection with hiv. skin test cl ... | 1990 | 2092414 |
| [fever, productive cough, chest pain]. | this 27 year old woman had thoracic pain associated with breathing and movements for 10 months. later on she complained about productive cough and fever. a pleural effusion developed. this effusion and the other symptoms did not resolve after therapy with erythromycin. microbiology, serology and a bronchoscopy were unrevealing. a thoracoscopy was performed yielding epithelioid granulomas in the biopsy specimen from the pleura. mycobacterium tuberculosis was grown from the pleural fluid. symptoms ... | 1990 | 2093221 |
| [aids pathology: various critical considerations (especially regarding the brain, the heart, the lungs, the hypophysis and the adrenal glands]. | our studies on pathology of aids point to four major conclusions. 1) the brain is often directly affected by the hiv infection (with the characteristics of subacute microglial encephalitis with pathognomonic multinucleated giant cells) and then by opportunistic infections such as cytomegalovirus, herpes-virus, papova-virus jc (with progressive multifocal leucoencephalopathy), mycobacterium tuberculosis, toxoplasma gondii, cryptococcus neoformans, candida albicans and aspergillus fumigatus; oppor ... | 1990 | 2094837 |
| [tumor necrosis serum induce plasmodium falciparum crisis form]. | this paper reports that tumor necrosis serum (tns) containing tumor necrosis factor (tnf) could inhibit plasmodium falciparum in vitro. tns was obtained from rabbits given macrophage-activating agent mycobacterium tuberculosis (bcg) followed by intravenous administration of bacterial endotoxin (lps). the results showed that tns dilutions of 1:3, 1:6 and 1:12 could induce disappearance of plasmodium falciparum (p less than 0.05) after being cultured 6 hours. tns decreased red blood cell infective ... | 1990 | 2099264 |
| [comparison of the results of transthoracic needle biopsy of the lungs using the nordenström and the rotex needles]. | in the diagnostics of nodular and infiltrative lung lesions in 28 patients the transthoracic needle biopsy of the lung was performed both by nordenström and rotex needle. in confirming the cancer, both needles were equally satisfactory. by rotex needle the tissue sample was obtained also from hard lesions from which the tissue sample with nordenström needle couldn't be obtained. by transthoracic needle biopsy of the lung the diagnosis of benign disease was confirmed in two patients with isolatio ... | 1990 | 2101945 |
| [an analysis of the mortality from extrapulmonary tuberculosis in the catchment areas of the community of valencia (1976-1980)]. | tuberculosis is a chronic infectious disease produced by mycobacterium tuberculosis or koch's bacillus, which mainly affects the airways and, less frequently, other organs in the body. tuberculosis is still a health and social problem in spain. the data of this study were obtained from "monografías sanitarias", "análisis de mortalidad", published by "generalitat valenciana", the standard mortality rate was calculated by direct methods. mortality is not the best sanitary parameter to study the re ... | 1990 | 2103267 |