| corynebacterium parvum stimulation of adherent and non-adherent cytotoxic cells in mice. | two naturally occurring cytotoxic cell populations have been identified in the peritoneal cavity of mice inoculated with c. parvum (cp), and are distinguishable on the basis of target-cell reactivity and intrinsic properties. the first effector cell was non-adherent to nylon wool and glass and non-phagocytic. these cells were selectively cytotoxic to the nk-sensitive target cell line k562, and present in the peritoneal cavity of mice 2 days after treatment with 700 micrograms of cp. the second c ... | 1981 | 7295509 |
| simple estimation of macrophage activity in tumour-bearing animals treated with corynebacterium parvum. | the effects of corynebacterium parvum on the reticuloendothelial system and on tumour growth have been known for many years. the causes of these effects have been the subject of many hypotheses and we here discuss a new method of evaluating macrophage activity and some of its components. these studies show that in macrophage activation the degree of enzymic activity and the cell size are more important than the number of cells present and that by giving silica concurrently it is macrophage enzym ... | 1981 | 7295527 |
| alterations of host resistance to listeria monocytogenes in tumour-bearing mice and the effect of corynebacterium parvum. | in tumour (fibrosarcoma)-inoculated mice, alterations of host resistance to listeria monocytogenes following tumour growth were examined. non-immune macrophage-mediated antibacterial resistance was severely suppressed up to day 4 or so after tumour inoculation, but was enhanced thereafter. on the other hand, t cell-mediated immune resistance retained the control level up to day 7 or so, but was suppressed thereafter. suppression of macrophage-mediated antibacterial resistance was not observed if ... | 1981 | 7298070 |
| late anaerobic haematogenous infection of experimental total joint replacement. a study in the rabbit using propionibacterium acnes. | | 1981 | 7298696 |
| comparative study on the immunostimulatory potency of different propionibacterium strains. | 198 strains of propionibacterium acnes, p. avidum, and p. granulosum were tested for their immunostimulatory potency using splenomegaly as index of stimulation, the activity differed widely from strain to strain. the antitumor and antimetastatic properties of six strains with differing stimulation activities were carefully examined. a close correlation between spleen enlargement and antitumor effects could be observed. the simple spleen enlargement test is recommended for determination of the im ... | 1981 | 7300800 |
| [immunochemotherapeutic treatment of gastrointestinal neoplasms]. | the results obtained with a three-fold association of drugs (vcr, 5fu, meccnu) in the treatment of 68 patients suffering from advanced carcinoma of the gastroenteric system followed up over an approximately four year period at the savona oncology centre are reported. the percentage of positive responses (cr + pr) was 75% for carcinoma of the colon-rectum and 44.4% for stomach cancer. the results obtained in intestinal carcinoma proved more satisfactory than those reported in the literature. this ... | 1981 | 7301183 |
| intrasplenic abscess: two case reports and review of the literature. | the occurrence of splenic abscess, sickle cell trait, and drug addiction is described in two patients. the coincidence of sickle cell disease and drug addiction in the possible etiology of splenic abscess should be noted. the first patient had a splenic abscess in the inferior pole of the spleen with signs and symptoms of left upper quadrant peritoneal irritation. the second patient had an intrasplenic abscess in the superior pole with pleuritic type chest pain and large left pleural effusion. i ... | 1981 | 7305137 |
| evaluation of intratumoral immunostimulants in the treatment of a transplantable rat mammary carcinoma. | | 1981 | 7308253 |
| adjuvant therapy for malignant melanoma. | | 1981 | 7313933 |
| antimetastatic effect of cell-wall skeleton of propionibacterium acnes c7 on lewis lung carcinoma in c57bl/6j mice. | the effect of the cell-wall skeleton (cws) of propionibacterium acnes c7 on the metastasis of lewis lung carcinoma in c57bl/6j mice was examined. intratumoral or intravenous injection of p. acnes-cws was effective for both prolongation of the survival period and protection against pulmonary metastases, but intraperitoneal injection was ineffective in tumor-bearing mice. intratumoral injection of p. acnes-cws also inhibited lymph node metastases and produced some complete tumor regressions. these ... | 1981 | 7319198 |
| effect of intracerebral administration of corynebacterium parvum on the growth of brain tumors in mice. | the effect of intracerebral administration of corynebacterium parvum (c. parvum) on the growth of syngeneic brain tumors in c57bl/6 mice and the mechanism of its action were investigated. mice were inoculated with 10(4) malignant glioma cells intracerebrally, and treated with various doses of c parvum on day 6 after tumor inoculation. the growth of tumors was significantly inhibited in proportion ot the dose of c. parvum. the cytotoxic activity of effector cells was enhanced when c. parvum was a ... | 1981 | 7319201 |
| resistance to cutaneous leishmaniasis in genetically susceptible balb/c mice. | when injected cutaneously with promastigotes of an isolate of leishmania tropica, balb/c mice develop progressive cutaneous disease whereas lesions in balb/c.h-2k mice heal after several weeks. lesions in balb/c mice injected into deep subcutaneous tissues with promastigotes are less obvious early but much more prominent later than in mice after strict intradermal injection. balb/c mice injected with corynebacterium parvum together with a preparation of frozen and thawed infected macrophages are ... | 1981 | 7325887 |
| differential sensitivity of two murine leukaemia sublines to cytolysis by corynebacterium parvum-activated macrophages. | we observed the growth of 2 sublines of leukaemia l1210 in histocompatible dba2 mice given 10(3) cells i.p. and studied the protective effect of corynebacterium parvum (cp). the growth of subline l1210-m was unaffected by pretreatment with cp or admixture with 10(5) peritoneal cells (pc) from cp-treated mice. in contrast, the growth of subline l1210-c was inhibited; cp pretreatment increased the proportion of long-term survivors (70% vs 20%) and admixture with cp-pc prolonged the survival time ( ... | 1981 | 7326193 |
| lower relapse rates after neighbourhood injection of corynebacterium parvum in operable cervix carcinoma. | the effect of adjuvant immunotherapy with a single neighbourhood injection of 2 mg c. parvum (cp) was investigated in a randomized study involving 43 patients with carcinoma of the cervix uteri, all of whom were treated by radical surgery. all patients had carcinoma confined to the cervix, the upper part of the vagina or the parametrial region. when the malignancy had spread to the parametrial region, additional postoperative radiotherapy was given. 22 patients received immunotherapy 10 days bef ... | 1981 | 7326194 |
| prostaglandin-like substances in propionibacterium acnes. vi. characterization of the lipid fraction by gas chromatography in conjunction with mass spectrometry. | the lipid fraction of p. acnes was submitted to stepwise purification followed by bioassay in order to localize the prostaglandin-like material. gc-ms analysis revealed the occurrence of substances having a part but not a total molecule in common with the prostaglandin family, suggesting that prostaglandin-like substances represent a new group of prostaglandin compounds. | 1981 | 7327228 |
| enhanced resistance of mice to mycoplasma pulmonis-induced arthritis by administration of killed corynebacterium parvum. | inoculation of mice with corynebacterium parvum 14 days before intraperitoneal inoculation of mycoplasma pulmonis resulted in arthritis of significantly lesser magnitude than in control mice as measured both clinically and histologically. mycoplasmas were isolated from the joints of mice inoculated with c. parvum less frequently than from control mice when the arthritis was maximal. mycoplasmas were also isolated in smaller numbers from the blood and joints of mice pretreated with c. parvum with ... | 1981 | 7337973 |
| depression of hepatic biotransformations by chemical immunoadjuvants. | administration of bcg and corynebacterium parvum is known to cause depression of the hepatic microsomal enzyme system (hmes) in mice. in the present study we explored the effects on hmes of two chemical immunoadjuvants, one of which (pyran copolymer) with peculiarly long-lasting biological activities. the two synthetic immunoadjuvants proved to be potent hmes inhibitors and, for pyran copolymer, peak levels of inhibition concurred with maximal macrophage activation. the inhibition was largely do ... | 1981 | 7338632 |
| research on the production site of tumor necrosis factor (tnf). | tumor necrosis factor (tnf) is an active component of serum taken from corynebacterium parvum (c. parvum) infected mice treated with lipopolysaccharide (lps). to locate the production site of tnf, we tried to block tnf production by using the following reagents; carrageenan, hydrocortisone, and trypan blue. following the injection of a large dose of carrageenan, administered before c. parvum treatment, tnf production was completely blocked. however, when administered after c. parvum treatment bu ... | 1981 | 7339069 |
| [the mechanism of immunosuppression in tumor-bearing mice--different susceptibility of various immune responses to immunosuppression and macrophage series as targets for immunosuppression (author's transl)]. | | 1981 | 7341394 |
| effects of glucose concentration n biomass, maximum specific growth rate and extracellular enzyme production by three species of cutaneous propionibacteria grown in continuous culture. | there cutaneous propionibacteria - propionibacterium acnes, p. avidum and p. granulosum - were grown in chemostats using semi-synthetic medium with various concentrations of glucose. biomass rose with increasing glucose concentration up to 0.3-0.4% (w/v) and then remained constant. propionibacterium granulosum had both a low yield and low mumax when grown in the absence of glucose suggesting that this organism is essentially 'saccharolytic' in its nutrition. in contrast, p. acnes and p. avidum h ... | 1981 | 7343643 |
| early changes in the radiosensitivity of corynebacterium parvum-stimulated cfu-s. | corynebacterium parvum injected intraperitoneally into c57bl mice induced a progression of hemopoietic cfu-s of c57bl mice from the resting state immediately after, and in the early period (approximately 8 h) after stimulation. dose-response survival curves of the stimulated endogenous spleen cfu-s after the c. parvum injection revealed changing slopes or d0 of dose-response survival curves indicative of changing sensitivity states. shortly after stimulation, the cells were sensitive to high spe ... | 1981 | 7348462 |
| anaerobic coryneform bacteria as normal flora of rabbit skin and regression of shope papillomas. | adult domestic rabbits were found to harbor anaerobic coryneform bacteria and possess serum agglutinins against these bacteria. the isolates from rabbit skin were characterized by morphology, anaerobiosis, catalase test, and gas chromatographic analysis of volatile acid products. preliminary findings on the effect of anaerobic coryneforms on the regression of shope papillomas have suggested that the life of such bacteria as normal flora enhances the regression. | 1980 | 7350547 |
| gastrointestinal ecology and histology of rats monoassociated with anaerobic bacteria. | pure cultures of anaerobic bacteria colonized the gastrointestinal tracts of germfree rats and induced an increase in the cellularity of intestinal tissue. | 1980 | 7356318 |
| improving the body's anticancer system. | | 1980 | 7356587 |
| macrophage-mediated suppression. i. evidence for participation of both hdyrogen peroxide and prostaglandins in suppression of murine lymphocyte proliferation. | macrophage-mediated suppression of con a induced proliferation of murine splenic lymphocytes was studied in vitro. either corynebacterium parvrum-induced peritoneal exudate cells (pec) or thioglycollate-induced pec could totally suppress lymphocyte proliferation at a pec:lymphocyte ratio of 2:10, whereas a ratio of 1 to 1.5: 10 caused a partial (60 to 68%) suppression. exogenous pge1 and pge2 at concentrations of 10(-8) to 10(-6) m could not totally suppress lymphocyte proliferation. conversely, ... | 1980 | 7356724 |
| evidence that the prostaglandin-like substances from propionibacterium acnes are not identical with pge2. | the prostaglandin-like substances (pls) isolated from p. acnes were investigated by reversed phase chromatography and gas chromatography-mass spectrometry. these analyses demonstrated that pls were not identical with pge2, which supports a concept of pls as a potential mediator of the inflammatory process in acne vulgaris. | 1980 | 7358133 |
| anaerobic botryomycosis. | botryomycosis ofthe liver developed in a patient receiving corticosteroid therapy. the botyromycosis was caused by propionibacterium acnes, which grew only anaerobically. the patient was successfully treated medically and at followup is asymptomatic. | 1980 | 7358842 |
| the role of host defense mechanisms in surgical infections. | as far as the immunologic responses and infections in surgery are concerned, there are many exciting areas of advancement at the present time. of these, nutritional factors seem of major importance. even though much has been learned in the past few years, there is much to be learned, particularly related to selected amino acid deficiencies and their interactions and the potential for fortification with certain amino acids in dietary therapy. whether the immune response can be made better than no ... | 1980 | 7361215 |
| mechanisms of c. parvum-induced coagulopathy in mice. | i.v. injection of corynebacterium parvum (cp) into c57bl and balb/c mice caused profound coagulation changes, featuring thrombocytopenia, decreased fibrinogen, increased fibrin/fibrinogen degradation products, and a concomitant microangiopathic haemolytic anaemia. these changes were greatest on the 9th day after cp, with recovery by day 21. i.p. injection caused similar effects but s.c. injection was ineffective. radiolabelled-platelet kinetics and distribution after i.v. cp indicated disseminat ... | 1980 | 7362773 |
| the activation of macrophages by corynebacterium parvum: effect of anti-complementary agents cobra venom factor and sodium cyanate. | | 1980 | 7365736 |
| influence of c. parvum on the effectiveness of passive serotherapy in the control of the el4 lymphoma in c57bl/6 mice. | administration of c. parvum alone did not improve the survival of c57bl/6 mice injected with the el4 lymphoma. the anti-tumour effect of anti-el4 ig was however increased by c. parvum treatment, and the combination therapy of anti-el4 ig and cytotoxic drugs was even more improved. however, c. parvum only had this effect when given by the same i.p. route as the tumour cells, and the effect was greater when c. parvum was injected 5 days before than 1 day after tumour cells. | 1980 | 7370164 |
| nephritis secondary to chronic cerebrospinal fluid - vascular shunt infection: "shunt nephritis". | chronic indolent shunt infection can lead to intermittent transient bacteremia and secondary deposition of immune complexes in the kidney. the infective organism is usually one of low virulence, resulting in vague symptoms and a delay in diagnosis. resolution of the infection usually results in return of renal function to normal. | 1980 | 7371442 |
| prostaglandin-like substances in propionibacterium acnes. iv. effect on isolated human vessels. | the present study demonstrates a powerful vasoconstrictor activity of prostaglandin-like substances (pls), extracted from p. acnes, on human blood vessels. pls is about equipotent to pge2 in its effect on human umbilical vessels, but the contractile response pattern is different. pls therefore seems to have specific and different physiological characteristics. | 1980 | 7371759 |
| biphasic protection against bacterial infection in mice induced by vaccination of propionibacterium acnes. | a single intraperitoneal injection of the phenol-treated cells of propionibacterium acnes into mice showed nonspecific resistance against subsequent lethal doses of an intraperitoneal challenge of klebsiella pneumoniae, staphylococcus aureus, and streptococcus pyogenes. the protection showed a biphasic pattern. the maximum protection, designated as the early phase protection, was seen in mice injected with p. acnes vaccine 1 to 2 days before the challenge, whereas the late phase protection was s ... | 1980 | 7380536 |
| investigation of the component of propionibacterium acnes (corynebacterium parvum) responsible for macrophage activation. | mouse peritoneal macrophages, activated with propionibacterium acnes (corynebacterium parvum) in vivo, exhibited altered morphological characteristics, increased acid phosphatase activity, and release of [eh]thymidine-labeled dna from tumor cells in vitro. comparison with macrophages from mice injected with a control organism, p. jensenii, showed that the morphological changes, but not acid phosphatase, correlated with the development of tumoricidal activity. investigation of the microbial compo ... | 1980 | 7380542 |
| [pleural effusion. local treatment by corynebacterium parvum]. | | 1980 | 7383874 |
| single versus multiple human-equivalent doses of c. parvum in mice: neutralization of the anti-metastatic effect. | the murine dose of i.v. c. parvum (466 microgram) was compared with a single, low, human-equivalent dose of 70 microgram and with repeated weekly low doses. all treatments increased the antibody titre against c. parvum (cp). however, repeated doses stimulated a much higher titre than single doses. in all treated animals spleen weight peaked at 2 weeks and then fell. a single low dose caused a 3-fold increase, a single high dose or multiple low doses a 6-fold increase. liver weight changes follow ... | 1980 | 7387830 |
| harmful effects of i.v. corynebacterium parvum given at the same time as cyclophosphamide in patients with squamous-cell carcinoma of the bronchus. | the effects are reported of a combination therapy of i.v. c. parvum and cyclophosphamide on the survival time and immune responses of patients with inoperable squamous-cell carcinoma of the bronchus. the immune status of the patients was evaluated by determining the antibody response to c. parvum, the e and eac rosettes, the pha response of blood lymphocytes, the skin-test reactivity to candida and ppd, the response to dncb and the chemotaxis and nbt-dye reduction capacity of neutrophil leucocyt ... | 1980 | 7387858 |
| facial follicular porphyrin fluorescence: correlation with age and density of propionibacterium acnes. | the incidence and distribution of facial follicular porphyrin fluorescence was studied in a large group of subjects ranging in age from 1 to 90 years. fluorescence appears first on the nose and chin, increases in incidence through adulthood and declines after the age of 50 possibly rflecting the ras population and confirmed that coproporphyrin iii is proed in vivo. | 1980 | 7387886 |
| treatment of cancer using corynebacterium parvum: similarity of two preparations in four animal tumor models. | the tumor inhibitory properties of corynebacterium parvum obtained from burroughs wellcome (cp-bw) or from institut merieux (cp-im) were compared in four animal tumor models: the cad2 mouse mammary carcinoma treated by intravenous (i.v.) or intratumoral (i.t.) injection of c. parvum; 13762a rat mammary adenocarcinoma treated by i.t. injection of c. parvum either alone or combined with excision of the primary tumor; lstra murine leukemia and line 10 cavian hepatoma, each treated with vaccines con ... | 1980 | 7397633 |
| macrophage heterogeneity in tumor resistance: cytostatic and cytotoxic activity of corynebacterium parvum-activated and proteose peptone-elicited rat macrophages against moloney sarcoma tumor cells. | | 1980 | 7397781 |
| growth and treatment of b 16 melanoma in hyperglycemic mice. | melanoma b-16 grew slowly in mice with hyperglycemia induced by alloxan, glucagon, or glucose. the mechanism of retarded tumor growth is different and depends on the origin of hyperglycemia. the concentration of immunoreactive insulin in blood of mice with melanoma and in the tumor tissue is increased in nondiabetic as well as in diabetic mice. the chemotherapy of melanoma in diabetic mice is as effective as in nondiabetic mice whereas immunotherapy in diabetic mice is not effective. combined ch ... | 1980 | 7403688 |
| aerobic and anaerobic bacteriology of cervical adenitis in children. | needle aspirates from 53 inflamed cervical lymph glands were studies for aerobic and anaerobic bacteria and mycobacteria. bacterial growth was achieved in 45 patients (85%). sixty-six bacterial isolates were recovered, averaging 1.5 isolates per specimen (0.8 aerobes and 0.7 anaerobes), with as many as 4 isolates in some specimens. aerobic organisms alone were recovered in 27 aspirates (60%) of the 45 culture-positive aspirates, anaerobic bacteria alone in 8 (18%), and mixed aerobic and anaerobi ... | 1980 | 7408372 |
| glucagon suppressed proliferation rate of mammary aplastic carcinoma in mice. | glucagon injected into mice with mammary aplastic carcinoma retards the growth and prolongs the mean survival time of the animals. glucagon stimulates the plaque-forming capacity and phagocytosis in tumor-bearing animals. cyclophosphamide treatment abolishes the antitumor effect of glucagon, while the effect of the hormone is enhanced in corynebacterium parvum pretreated animals. it follows that the tumor-retarding effect of glucagon are mediated by mainly by maintaining high b-type reactivity a ... | 1980 | 7409736 |
| the use of immunotherapy to enhance tumor rejection immunity in leukemic (l2c) guinea pigs following remission induction with meccnu. | | 1980 | 7412356 |
| anti-t antibody in malignant melanoma patients. influence of response and survival following chemotherapy--changes in serum levels following c parvum, bcg immunization. | the level of anti-t antibodies directed towards the precursor t antigen of the mn blood group system was measured in the sera of 55 patients with disseminated melanoma, before and during chemoimmunotherapy. the anti-t titer was subnormal in patients before therapy; patients who responded to therapy had significantly higher titers than did nonresponders in sera taken before therapy, at regression/progression of disease, and during the last pulse of treatment. higher pretreatment titers were assoc ... | 1980 | 7417939 |
| purification and partial characterization of an acid phosphatase (ec 3.1.3.2) produced by propionibacterium acnes. | a strain of propionibacterium acnes (type i; marples & mcginley, 1974), isolated from a blackhead acne lesion, produced an acid phosphatase which was present in the culture supernatant in the late-exponential and early-stationary phases of growth. this acid phosphatase was purified more than 45 000-fold (4.5% yeild). the purified enzyme gave two protein bands on sodium dodecyl sulphate-polyacrylamide gel electrophoresis corresponding to molecular weights of 155 000 and 87 100. the enzyme had a s ... | 1980 | 7420057 |
| effect of three strains of propionibacteria (p. granulosum, p. avidum, p. acnes) and cell-wall preparations on lymphocytes and macrophages. | the effect of the strains of propionibacteria (p. granulosum, p. avidum, p. acnes) on functional and metabolic activities of mouse lymphocytes and rabbit macrophages was examined in vivo and in vitro. in some of the experiments bacterial cell walls isolated from the above bacteria were tested and compared with the activity of whole bacteria. in isolated lymph-node lymphocytes influx of 86rb and its inhibition by ouabaine, release of 86rb and 51cr from prelabelled cells, as well as spontaneous an ... | 1980 | 7424239 |
| effect of intravenous corynebacterium parvum on peripheral-blood effector cells of cancer patients. | the i.v. administration of corynebacterium parvum (cp) to patients who had recently undergone resection of colorectal tumours was found to have the following effects: 1. polymorphonuclear leucocyte counts were raised 24 h after cp administration, while both lymphocyte and monocyte counts fell during this period. polymorph and lymphocyte counts had returned to pre-infusion levels at one week, but monocyte counts were significantly increased at this time. 2. the lymphocyte mitotic response to pha ... | 1980 | 7426303 |
| characterization of serum-independent polymorphonuclear leukocyte chemotactic factors produced by propionibacterium acnes. | the size and production of polymorphonuclear leukocyte (pmn) chemotactic factors by propionibacterium acnes has been studied. all eight strains of p. acnes which were tested liberated pmn chemotactic factors in their growth culture media. the factor(s) produced by one strain, 6919, were studied in greater depth. the pmn response was proportional to the dose of culture supernatant and chemotactic activity increased with the duration of p. acnes culture. the neutrophil migration towards culture su ... | 1980 | 7429606 |
| recovery of hemopoiesis in lethally irradiated mice after treatment with propionibacteria. | three investigated strains of propionibacteria (p. acnes, p. granulosum and p. avidum) injected intraperitoneally in doses of 1.5 mg per mouse, significantly prolonged survival of lethally (8.50 r) irradiated mice. p. granulosum, being the most effective in increasing survival of irradiated mice was chosen for the studies on the activity of bone-marrow pluripotent stem cells. the number of endogenous spleen colonies was significantly increased in mice injected with p. granulosum 3 days prior to ... | 1980 | 7434382 |
| anaerobic bacteremia in children. | twenty-nine anaerobic isolates were recovered from 28 pediatric patients with anaerobic bacteremia: 14 bacteroides sp (11 in the b fragilis group); four anaerobic gram-positive cocci; four clostridium sp; four propionibacterium acnes; and three fusobacterium sp. no aerobic bacteria were isolated from these patients. the gastrointestinal tract was the possible portal of entry in 13 instances, eight of which were due to bacteroides organisms, four to clostridium sp, and one to f nucleatum. the ear ... | 1980 | 7435464 |
| an analysis of sebum excretion rate, bacterial population and the production rate of free fatty acids on human skin. | bacteria were sampled using a "scrub" technique from the skin surface of the faces of forty-nine female subjects aged 18-21 years. the sebum excretion rate was determined by a gravimetric method and the level of free fatty acids by titration. the production rate of free fatty acids was calculated from the product of the concentration of free fatty acids in the sebum and the sebum excretion rate. the date was analysed using kendall's rank correlation method. positive correlations existed between ... | 1980 | 7437304 |
| antitumor activity of corynebacterium parvum and retinyl palmitate used in combination on the lewis lung carcinoma. | the effects of corynebacterium parvum and retinyl palmitate given at various levels, schedules, and routes of administration on primary lewis lung carcinoma and its metastases have been evaluated in c57bl/6j mice given s.c. inoculations of 5 x 10(5) tumor cells. single i.v., but not i.p., s.c., or i.m., administration of c. parvum (0.35 mg/mouse given on days 0, 1, or 3) reduced growth of tumor and prolonged survival time. retinyl palmitate (3000 iu/mouse/day) given alone i.p. either before, aft ... | 1980 | 7438095 |
| effect of corynebacterium parvum stimulation on granulopoiesis. | | 1980 | 7439223 |
| structure and function of human effusion macrophages from patients with malignant and benign disease. 2. in vitro cytostatic and cytolytic effect on human tumour cell lines. | human effusion macrophages isolated from the pleural or ascitic effusions of 14 patients with malignant or benign disease usually inhibited methyl-3h-thymidine incorporation in an adherent human tumour cell line (nhik 3025) when the macrophages were challenged with target cells immediately after isolation. the cytostatic activity disappeared when the macrophages were cultured for 18 hours in vitro before target cell challenge. the presence of endotoxin (lps) or corynebacterium parvum (cp) during ... | 1980 | 7446129 |
| in vitro response to corynebacterium parvum of human effusion lymphocytes isolated from patients with malignant and benign disease. | non-adherent effusion cells (ec), mostly lymphocytes, were isolated from the pleural effusions of 8 patients with malignant and 7 patients with benign disease. corynebacterium parvum (cp) induced increased methyl-3h-thymidine (methyl-3h-tdr) incorporation in ec cultures, but the response was lower than that usually found with autologous or allogeneic normal human blood lymphocytes. experiments with highly purified effusion lymphocytes indicated that the response to cp was influenced by the prese ... | 1980 | 7446130 |
| endocarditis due to propionibacterium acnes. | a case of propionibacterium acnes endocarditis documented by blood culture and culture of the valve is presented. a brief review of the literature is summarized. | 1980 | 7446476 |
| enhancement of the antitumor-activity of corynebacterium parvum by appropriate adjustment of dosage schedules. | single and multiple doses of corynebacterium parvum ranging from 0.01 to 60 mg/kg as the total dose were tested at selected times against 10(6) p388 leukemia cells inoculated ip in bdf1 mice. the minimum dose needed for maximum protection was determined according to the ability of the treatment to produce an increase in the lifespan (ils) of mice. the ip dose required after transplant for significant ils was 40 mg/kg as the total dose. the administration of a single dose 2 days prior to implant ... | 1980 | 7448829 |
| a modified spectrophotometric test for nitroblue-tetrazolium reduction of granulocytes and monocytes and its application for testing inhibition of monocytes by serum components in tumor patients. | for evaluation of serum-factor involvement in monocyte function, we established a modified quantitative spectrophotometric-nitroblue tetrazolium (nbt) test. we investigated the stimulation of human peripheral monocytes and granulocytes by zymosan, endotoxin, and corynebacterium parvum in vitro. in addition, polymorphonuclear neutrophil (pmn) function in a patient with non-hodgkin lymphoma was studied during the course of polychemotherapy and intravenous application of corynebacterium parvum (c. ... | 1980 | 7450815 |
| optimal conditions for antitumor activity of c. parvum against the ascites form of sarcoma 180. | single and multiple doses of corynebacterium parvum (c. parvum) ranging from 0.1--60 mg/kg were tested for antitumor activity against 10(6) sarcoma 180 cells in male cd1 mice. determinations were made of the optimal dose and time of treatment needed to produce maximum suppression of the tumor using both median survival time and percent survival to day 90 as endpoints. a dose of 1 mg/kg given 3 days before sarcoma 180 transplant produced complete protection (100% survival). all other treatment re ... | 1980 | 7452006 |
| [pathology caused by anaerobic bacteria in pediatrics. our cases]. | | 1980 | 7453681 |
| anaerobic bacterial coxitis and pseudocystic tumour in rheumatoid arthritis. a case report. | when patients with rheumatoid arthritis develop rapidly growing tumours around the hip or elbow joint, the possibility should be considered that this condition could be pseudocystic with a low virulent infection. the present report describes such a case of anaerobic bacterial coxitis with rapidly growing tumour. | 1980 | 7455634 |
| improved survival in severely burned animals using intravenous corynebacterium parvum vaccine post injury. | this study was designed to determine the potential benefit or toxicity of an immunomodulator, corynebacterium parvum vaccine, when it is given after severe burn injury. forty conditioned beagles received a 33% total body surface 3-degree flame burn and were resuscitated with ringer's lactate solution (3 ml/kg/% burn). wounds were treated daily for 10 days with silver sulfadiazine cream. two days and nine days after burn, 21 of the animals received c. parvum vaccine (10 mg/kg iv) in a saline infu ... | 1981 | 7455909 |
| fermentation of 1,2-o-iso-propylidene-d-glucofuranose ("monoacetone glucose") by anaerobic bacteria. | various species of clostridium, bacteroides, propionibacterium and eubacterium were incubated in a 1% solution of 1,2-o-iso-propylidene-d-glucofuranose in a peptone-yeast-extract (py) medium according to the vpi-technique. the volatile and non volatile acids were investigated by gas-liquid chromatography. all microorganisms showed a pattern of vfa different from that one produced by the glucose containing medium. in most microorganisms the formation of acetic acid was suppressed while the produc ... | 1980 | 7456850 |
| [comparison of two nonspecific immune stimuli in antiblastic chemotherapy]. | antiblastic chemotherapy was combined with two aspecific immune stimuli, levamisole corynebacterium parvum, in 48 and 55 neoplastic patients respectively. c. parvum was found to be more easy to handle, particularly since levamisole, albeit compensated by absolute lymphocytosis in many cases. in addition, c. parvum proved more effective in patients with lung and oro-pharyngeal neoplasias, whereas levamisole appeared to be of greater use in breast cancer. both stimuli led to improved coenaesthesia ... | 1980 | 7458652 |
| the antitumour activity of maltose tetrapalmitate compared with other immunoadjuvants, and its effectiveness after tumour surgery. | the effectivenss of maltose tetrapalmitate (mtp) as an antitumour immune adjuvant was verified by its comparison with other known immunopotentiators, namely bcg, corynebacterium parvum, levamisole and pyran copolymer. copenhagen x fisher 344/crbl f1 hybrid male rats inoculated s.c. with the dunning r3327a prostatic adenocarcinoma were used as the test system. all animals treated with immunoadjuvants showed a delay in tumour appearance and inhibition of early tumour growth. mtp was found to be th ... | 1980 | 7459208 |
| effects of corynebacterium parvum on cellular immunity of cancer patients, assayed sequentially over 63 days. | the immunologic effects of corynebacterium parvum (2 mg/m2) infused at three-week intervals were determined by 19 sequential assays in each of nine patients with metastatic malignancy. peripheral blood immunologic profiles were followed for 63 days. no statistical significant changes (p greater than 0.05) occurred in total white cell, lymphocyte, or monocyte counts. subpopulation assays demonstrated significant increases in e rosetting cells but not in sig-bearing cells; there was a correspondin ... | 1981 | 7459817 |
| immunomodulation by corynebacterium parvum in two strains of guinea-pigs and the effect of cyclophosphamide. | an early immunosuppressive phase in the overall stimulatory effect of corynebacterium parvum on the immune response of two strains of guinea-pigs to ovalbumin (oa) is described. boosters given soon after treatment with c. parvum elicited lower secondary responses than those given later on, the peak secondary antibody titre increasing with the interval between primary immunization and the booster injection. transfer of blood leukocytes (lymphocytes) of immunized donors to virgin syngeneic recipie ... | 1981 | 7461727 |
| the correlation of susceptibility of different propionibacterium strains to macrophage killing and antitumor activity. | the stimulation of the reticuloendothelial system and antitumor activity against sarcoma-l-1 of three different strains of propionibacterium was investigated. the bacteria injected intraperitoneally with a single dose of 1 mg per mouse appeared to possess different effectiveness in both assays used. the same strains were examined for their susceptibility to phagocytosis and intracellular killing by murine peritoneal macrophages. the results obtained showed correlations between propionibacterium ... | 1980 | 7464684 |
| new medium for isolating propionibacteria and its application to assay of normal flora of human facial skin. | the conditions for isolation and cultivation of propionibacterium acnes and related propionibacteria were studied in detail. triton x-100 added to the diluent inhibited the growth of propionibacteria in concentrations of 0.05 to 0.1%. however, such was not the case with tween 80; rather, growth of the bacteria was further enhanced by this agent. consequently, tween 80 was considered to be a suitable surfactant for addition to the diluent for isolation of propionibacteria. a new medium for isolat ... | 1980 | 7470244 |
| macrophage functions in beige (chédiak-higashi syndrome) mice. | beige (c57bl/6j-bgj/bgj) mice are the murine counterpart of the chédiak-higashi syndrome, exhibiting abnormal lysosomes in phagocytes. these mice, however, responded normally to corynebacterium parvum killed bacterial vaccine with splenomegaly and an increase in peritoneal macrophages. the c. parvum-elicited macrophages showed normal immunoglobulin g fc- and c3b-mediated rosettes and phagocytosis. the antitumor action of the macrophages was assessed against the lewis lung carcinoma, a tumor that ... | 1980 | 7471044 |
| comparative effects of different strains of corynebacterium parvum on natural cell-mediated cytotoxicity. | because there are conflicting reports regarding the effects of corynebacterium parvum (cp) on natural killer (nk) cell activity, several different strains of cp were compared. in replicate experiments, age- and sex-matched mice received 0.25-mg i.p. injections of one of four types of cp; formalin-killed strain 6134; heat-killed strain 6134; formalin-killed strain 5888 (actually corynebacterium granulosum); or formalin-killed cp from the pasteur institute. at various days thereafter, two to three ... | 1980 | 7471057 |
| effect of exercise on endotoxin shock with special reference to changes in concentration of cytokines. | the effects of exercise on susceptibility to endotoxin shock and the serum levels of interleukin-1 (il-1) and interleukin-6 (il-6) were studied in mice primed with propionibacterium acnes (p. acnes, 1.0 mg). endotoxin shock was induced by injection of lipopolysaccharide (lps, 0.2 mg) 7d after the priming injection of p. acnes. the exercise-loaded mice performed voluntary exercise for 120 min on a running-wheel and lps was injected into the tail vein immediately after the end of exercise. two of ... | 1995 | 7474534 |
| cloning of a new cytokine that induces ifn-gamma production by t cells. | the mechanism underlying the differentiation of cd4+ t cells into functionally distinct subsets (th1 and th2) is incompletely understood, and hitherto unidentified cytokines may be required for the functional maturation of these cells. here we report the cloning of a recently identified ifn-gamma-inducing factor (igif) that augments natural killer (nk) activity in spleen cells. the gene encodes a precursor protein of 192 amino acids and a mature protein of 157 amino acids, which have no obvious ... | 1995 | 7477296 |
| adhesion molecules on intermediate tcr cells. ii. hepatoprotective effects of hyaluronic acid on acute liver injury. | the liver is a major organ wherein extrathymic t cells and nk cells exist in mice. due to their unique properties, i.e., extrathymic t cells are tcr (or cd3)-intermediate+ il-2r beta+ (herein termed intermediate tcr cells) and nk cells are tcr(-)il-2r beta+, they are easily distinguished from the other lymphocyte subsets by using mabs in conjunction with immunofluorescence tests. they were recently found to express a higher level of cd44 antigen, which is a ligand for hyaluronic acid, than that ... | 1995 | 7497529 |
| il-10 is involved in the protective effect of dibutyryl cyclic adenosine monophosphate on endotoxin-induced inflammatory liver injury. | the effects of exogenous camp, dibutyryl camp (dbcamp) on lps-induced liver injury were examined in mice made hypersensitive to lps by treatment with i.v. injection of propionibacterium acnes. in vivo administration of dbcamp significantly protected p. acnes-treated mice from lps-induced liver injury, including apoptosis of hepatocytes. dbcamp significantly increased circulating il-10 level in correlation with suppression of the tnf-alpha level after lps challenge in p. acnes-treated mice. treat ... | 1995 | 7499862 |
| role of vascular nitric oxide synthase in endotoxin shock of propionibacterium acnes-sensitized rats. | the sensitivity of animals to endotoxin differs significantly between species. thus, factors that determine the susceptibility to endotoxin may play important roles in the pathogenesis of septic shock. in order to determine the mechanism responsible for susceptibility to endotoxin, the effect of lipopolysaccharide (lps) on the circulatory status of propionibacterium acnes (pa)-sensitized rats was studied. following the intravenous administration of a low dose of lps, the arterial blood pressure ... | 1994 | 7518220 |
| b7-1/cd80-transduced tumor cells elicit better systemic immunity than wild-type tumor cells admixed with corynebacterium parvum. | tumor cells genetically modified by transduction of b7 (b7-1/cd80), a natural ligand for the t-cell costimulatory molecules cd28 and ctla-4, can elicit potent tumor immunity, and they can be effective for treatment of established cancers in animal models. in this study, three tumor lines, the el4 lymphoma, the p815 mastocytoma, and the mca102 sarcoma were transduced with recombinant retrovirus containing the murine b7 gene, and their potency to induce systemic immunity protective against challen ... | 1994 | 7522958 |
| cellular localization of inducible nitric oxide synthase in experimental endotoxic shock in the rat. | 1. endotoxin induces a shock-like syndrome with increased nitric oxide synthesis. to clarify the cellular source of no in endotoxic shock we used immunohistochemistry and in situ hybridization to localize inducible no synthase in rats given lipopolysaccharide or corynebacterium parvum and lipopolysaccharide. immunohistochemistry was carried out with an antibody raised against a synthetic peptide of mouse macrophage no synthase. in situ hybridization was performed with 35s-labelled oligonucleotid ... | 1994 | 7523018 |
| inhibition of nitric oxide synthesis enhances the expression of inducible nitric oxide synthase mrna and protein in a model of chronic liver inflammation. | in septic shock the inhibition of inducible nitric oxide synthase (inos) could be of therapeutic value. however, side effects have to be investigated. therefore we studied the effects of chronic nos inhibition on the level of inos expression in a model of chronic liver inflammation induced by corynebacterium parvum (c. parvum) which causes sustained inos expression in the liver. nos inhibitors decreased the rise in plasma levels and urinary excretion of nitrite/nitrate by about 50%; however, ino ... | 1994 | 7526851 |
| mum-4, a monoclonal antibody reacting with resident peritoneal mouse macrophages. | a new rat monoclonal antibody, mum-4, which recognizes a murine antigen/epitope that is absent on monocytes, strongly expressed on resident peritoneal macrophages and almost completely absent from peritoneal macrophages 4 days after an intraperitoneal injection of thioglycollate or heat-killed propionibacterium acnes organisms, is described. immunocytochemistry and flow cytometry have been used to characterize the specificity of the antibody. mum-4 did not react with blood granulocytes, peritone ... | 1995 | 7535062 |
| sequential induction of nitric oxide synthase by corynebacterium parvum in different organs of the mouse. | 1. the ability of corynebacterium parvum (c. parvum) to induce nitric oxide (no) synthase in the macrophage, spleen, liver, aorta, heart and brain, and to elevate plasma no2-/no3- in the mouse was investigated. in addition, the relationship between no synthase activity and blood pressure was studied. 2. c. parvum (100 mg kg-1, i.p.) induced a time-dependent expression of a ca(2+)-independent no synthase in the macrophage, spleen, liver, aorta and heart. the time course of induction of the no syn ... | 1995 | 7537593 |
| biological response modifiers (brm) as antigens. iii. t cell lines specific for brm kill tumor cells in a brm-specific manner. | in order to investigate tumoricidal effector cells in therapy by biological response modifiers (brm) such as propionibacterium acnes, bacillus calmette-guérin (bcg), streptococcus pyogenes and a protein-bound polysaccharide (psk), we established t cell lines specific for each brm from balb/c mice immunized with the corresponding brm. these t cell lines proliferated and produced interleukin-2 (il-2) and/or il-4, but only in the presence of the relevant brm and balb/c spleen cells as the antigen a ... | 1995 | 7538449 |
| lymphocytes infiltrating the cns during inflammation display a distinctive phenotype and bind to vcam-1 but not to madcam-1. | the nature of inflammatory lymphocytes recruited to the cns has been studied in a model of chronic inflammation. injection of killed corynebacterium parvum into the cortex of the mouse brain produces a circumscribed inflammatory cellular infiltrate around the injection site, and recruited mononuclear inflammatory cells (ic) can be isolated for flow cytometric analysis. the majority of ic were t cells. in comparison with the predominant naive population of mesenteric lymph node t cells, ic t cell ... | 1995 | 7540864 |
| induction of proinflammatory cytokines by a soluble factor of propionibacterium acnes: implications for chronic inflammatory acne. | although many cytokines have been implicated in the development and persistence of inflammatory immune responses, it is unknown if any of these are important in inflammatory acne. this study investigated the production of the proinflammatory cytokines interleukin-8 (il-8), il-1 beta, and tumor necrosis factor alpha (tnf-alpha) by human monocytic cell lines, thp-1 and u937, and by freshly isolated peripheral blood mononuclear cells from acne patients. both propionibacterium acnes and supernatants ... | 1995 | 7542639 |
| minimum bactericidal concentrations of propionibacterium acnes isolates from cases of chronic endophthalmitis. | six isolates of propionibacterium acnes recovered from cases of chronic infectious endophthalmitis following extracapsular cataract extraction were tested for antibiotic susceptibility. all isolates were susceptible to penicillin, cefazolin, and vancomycin with a macrobroth dilution method. minimum bactericidal concentrations testing at 72 h revealed that six of six isolates were killed by < or = 1.0 micrograms of vancomycin/ml, one of six isolates by < or = 1.0 micrograms of penicillin/ml, and ... | 1995 | 7554799 |
| a novel costimulatory factor for gamma interferon induction found in the livers of mice causes endotoxic shock. | administration of monoclonal anti-cd3 antibody to mice treated with propionibacterium acnes induced secretion of a high level of gamma interferon (ifn-gamma) into the circulation system, while it induced no significant release in untreated mice. in order to analyze this high-level induction of ifn-gamma in these bacterium-treated mice, we investigated the factors that might be involved. an activity that induces ifn-gamma in t cells was observed in the liver extracts of mice treated with p. acnes ... | 1995 | 7558306 |
| antimicrobial effects of tea-tree oil and its major components on staphylococcus aureus, staph. epidermidis and propionibacterium acnes. | major components of two tea-tree oil samples were identified using thin layer and gas-liquid chromatography (tlc and glc). using a tlc-bioautographic technique, the tea-tree oils, terpinen-4-ol, alpha-terpineol and alpha-pinene were found to be active against staphylococcus aureus, staph. epidermidis and propionibacterium acnes whereas cineole was inactive against these organisms. the mic values of the three active compounds increased in the order alpha-terpineol < terpinen-4-ol < alpha-pinene f ... | 1995 | 7576514 |
| deformability and cd11/cd18 expression of sequestered neutrophils in normal and inflamed lungs. | neutrophil (pmn) sequestration in the pulmonary microvasculature precedes the migration of these cells into the airspaces in inflamed lungs. intratracheal instillation of the heat-killed organism corynebacterium parvum in the rat induces an alveolitis in which pmn constitute 70 to 80% of the total cell count in the bronchoalveolar lavage (bal). this acute alveolitis results in increased sequestration in the pulmonary microvasculature of 51cr-labeled pmn when compared with control lungs. the aims ... | 1995 | 7576688 |
| induced regression of bovine papillomas by intralesional immunotherapy. | it has long been assumed that papilloma regression is mediated by immunological mechanisms which are probably cellular in nature. the potentiation of these responses may alter the course of papilloma progression. certain strains of the bacterium corynebacterium parvum (propionibacterium acnes) have been shown to augment cellular immune mechanisms by increasing both macrophage and natural killer cell activity. this study involves the use of naturally occurring bovine papillomas to investigate the ... | 1994 | 7584507 |
| a synthetic inhibitor of interleukin-1 beta converting enzyme prevents endotoxin-induced interleukin-1 beta production in vitro and in vivo. | a potent, reversible, tetrapeptide inhibitor of interleukin-1 beta converting enzyme (ice), l-709,049, has been shown to suppress the in vitro production of mature il-1 beta. we now report that this inhibitor also effectively suppresses the production of mature il-1 beta in a murine model of endotoxic shock. intraperitoneal administration of l-709,049 reduced the elevations of il-1 beta in the plasma and peritoneal fluid of mice treated with lps in a dose-related manner (ed50 = 2 +/- 0.9 mg/kg). ... | 1995 | 7584670 |
| propionibacterium acnes endocarditis. | propionibacterium acnes endocarditis is a rare disease. a case of prosthetic valve endocarditis involving p acnes complicated by multiple mycotic aneurysms is reported. a brief review of the literature is presented. | 1995 | 7585277 |
| prevention of endotoxin-induced acute lethality in propionibacterium acnes-primed rabbits by an antibody to leukocyte integrin beta 2 with concomitant reduction of cytokine production. | acute lethality was induced in rabbits by the sequential injection of propionibacterium acnes and lipopolysaccharide (lps). p. acnes induced the infiltration of inflammatory cells into the liver lobules during the early phase, and lps in the late phase caused death in association with pathological changes mimicking hepatocellular necrosis or degeneration around infiltrated mononuclear cells and fibrin deposition in the liver, lung, and kidney, suggestive of a systemic schwartzman-like reaction. ... | 1995 | 7591140 |
| effective immune protection of pigs against cysticercosis. | a scolex protein antigen (spa) was prepared from cysticerci of taenia solium obtained from naturally infected pigs. yorkshire pigs were vaccinated with spa plus incomplete freund's adjuvant (ifa) or with spa plus corynebacterium parvum (cp). controls were given ifa plus phosphate-buffered saline (pbs) or cp plus pbs. all animals were given three subcutaneous injections at 20-day intervals. ten days after the third injection, the pigs were fed with 10(4) viable eggs of t. solium. all pigs develop ... | 1995 | 7604530 |
| [care of intrapleural corynebacterium parvum in malignant pleural effusion]. | | 1994 | 7614613 |
| preparation of specific antibodies against murine il-1ra and the establishment of il-1ra as an endogenous regulator of bacteria-induced fulminant hepatitis in mice. | blocking monoclonal antibodies (mabs) specific to mouse interleukin-1 receptor antagonist (il-1ra) were prepared by immunizing armenian hamsters with recombinant mouse il-1ra. a sensitive and specific elisa against mouse il-1ra was also established. in propionibacterium acnes-induced liver injury, p. acnes induced transient increase of serum tumor necrosis factor-alpha levels but not those of il-1ra, il-1, and il-6. however, subsequent lipopolysaccharide (lps) challenge induced the increase of s ... | 1995 | 7616110 |
| inflammation in acne vulgaris. | recent findings suggest that an overly vigorous immune response to propionibacterium acnes may be the fundamental problem in patients with inflammatory acne. these data and evidence for the antiinflammatory effects of acne medications are reviewed. | 1995 | 7622652 |
| the role of tumor necrosis factor in increased airspace epithelial permeability in acute lung inflammation. | increased airspace epithelial permeability is an early event in lung inflammation and injury. in this study, we have developed a rat model to study the mechanisms of the epithelial permeability to 125iodine-labeled bovine serum albumin (125i-bsa), instilled intratracheally during acute lung inflammation. epithelial permeability was measured as the percentage of instilled 125i-bsa appearing in the blood. the increase in epithelial permeability induced by intratracheal instillation of heat-killed ... | 1995 | 7626286 |
| neutrophil sequestration in rat lungs. | the transit of neutrophils through the pulmonary microvasculature is prolonged compared with red blood cells and is increased further during cigarette smoking and in exacerbations of chronic obstructive pulmonary disease. the increased residence time (sequestration) of neutrophils in the pulmonary capillaries in these conditions may be the first step leading to the accumulation of cells within the lung interstitium and in the bronchoalveolar space, so potentiating lung damage. a rat model has be ... | 1995 | 7638810 |