| viability of vibrio cholerae biotype el tor and of cholera phage on vegetables. | different vegetables were contaminated with sewage water containing large amounts of el tor vibrios, and the viability of these vibrios was studied under defined conditions of storage. during the dry season (summer) in jerusalem, the el tor vibrios survived for up to 24 hr on parsley, 24 to 30 hr on tomatoes and carrots, 24 to 48 hr on cucumbers, peppers and okra and two to three days on lettuce, when the vegetables were stored at room temperature. the survival time was longer during the rainy s ... | 1975 | 1184361 |
| metabolism of [5-t]fructose by isolated liver cells. | glucose formed from [5-t]fructose in rat hepatocytes contains about 10 to 30% tritium. this does not appear to be due to fructose metabolism via hexokinase since neither the initial presence of glucose, nor wide variations in the original fructose concentration, have much effect on the relative labeling of glucose versus water from [5-t]fructose. comparison of the t:14c ratios in glucose produced from [u-14c, 5-t]fructose and d-[u-14c, 2-t]glyceraldehyde indicate that there is tritium retention ... | 1975 | 1184583 |
| effects of cholera toxin on adenylate cyclase. studies with guanylylimidodiphosphate. | similarities exist between the properties of adenylate cyclase after stimulation by cholera toxin and after stimulation by guanylylimidodiphosphate (gpp-(nh)p). thus a strong stimulation is achieved by both agents, the stimulation is essentially irreversible, the action of certain hormones is enhanced and the enzyme can be solublized with lubrol px in the activated state. because of these similarities the interaction of cholera toxin and gpp(nh)p on adenylate cyclase was examined. it was found t ... | 1975 | 1184756 |
| microbiological examination of ship sewage for the presence of vibrio cholerae. | | 1975 | 1191809 |
| pharmacodynamic action of antygen vi. | | 1975 | 1191811 |
| selective inhibition of cholera toxin- and catecholamine-stimulated lipolysis by blocking agents. | vibrio cholerae enterotoxin stimulates lipolysis in rat epididymal fat cell suspensions. like hormones this toxin increases adenylate cyclase activity, raising levels of cyclic adenosine 3',5'-monophosphate (camp), which activates a cellular lipase. using specific blocking agents, we studied the responses to the adrenergic lipolytic hormones epinephrine, norepinephrine, and isoproterenol, and to cholera toxin. all stimulators were used at 100 x threshold dose. propranolol (34 mum), a beta blocki ... | 1975 | 1193734 |
| laryngeal structure following microcauterization. | the paper supports and reaffirms the objectives of contemporary laryngology in the treatment of cancer of this organ. these objectives are, first and foremost, the eradication of the tumor, and second, the preservation of function. a relatively recent contribution to the "state of the art" has been the development of the field of microlaryngology. the introduction of the surgical microscope has provided the laryngologist with better means to judge the type, localization and extent of neoplastic ... | 1975 | 1195967 |
| comparative in vivo distribution of opiate agonists and antagonists by means of double isotope techniques. | | 1975 | 1196002 |
| synchronization of the fission yeast schizosaccharomyces pombe using heat shocks. | | 1975 | 1196141 |
| pharmacological investigations on elaeocarpus ganitrus. | | 1975 | 1197422 |
| immunotherapy with neuraminidase-treated tumor cells after radiotherapy. | | 1975 | 1197655 |
| [influence of qualitatively different diets on the reproductive capacity and function of the adrenal cortex in rats with experimental toxic hepatitis]. | the effect of a diet rich in protein and the group b vitamins on the function of the ovaries and adrenal cortex, as well as on the reproductive capacity of animals with experimental affection of the liver produced by administration of carbon tetrachloride was studied on 115 mongrel albino female-rats. a high-standard semisynthetic diet devised at the institute of nutrition of the ams of the ussr and three isocaloric experimental diets calorifically enriched by 20, 50 and 100 per cent and contain ... | 1975 | 1199012 |
| [changes in the lungs of white mice under the action of cholera exotoxin]. | | 1975 | 1199577 |
| isolation of single cell nuclei from human epidermis for cytophotometric dna--measurements. | diluted acetic acid was applied to dissociate human epidermis into its constituents. a simple procedure to gain isolated single cells for cytophometric determination of their nuclear dna content is described. the method is suited for single cell cytophotometric measurements and for cytofluorimetric flow systems likewise. the losses of feulgen--stainable nuclear dna during preparation were studied in dependence on the incubation time of the cells in acetic acid. the kinetics of extraction of nucl ... | 1975 | 1200706 |
| location and the primary structure around the disulfide bonds in cholera toxin. | | 1975 | 1201095 |
| dermo--epidermal adhesion and its effect on epidermal structure in the mouse. | evidence is presented that dermo--epidermal adhesion is low in mouse ear. consequently the pressure caused by mitotic activity in the basal epidermal layer is readily relieved by cell extrusion distally and the number of basal cells per unit skin area remains low. the rule then is that the number of distal cells per unit skin area must be equally low, and the epidermis is therefore thin. conversely, dermo--epidermal adhesion is high in mouse sole-of-foot epidermis, and the mitotic pressure leads ... | 1975 | 1201191 |
| an improved method of assessing topical corticosteroid activity. | topical application of ointment bases causes varying degrees of epidermal thickening in guinea-pigs. this is reproducible and can be accurately measured. suppression of these changes was produced by addition of beta-methasone 17-valerate, fluocinolone acetonide, fluocinonide and hydrocortisone acetate. the inhibitory effect of these steroids was related to the type of corticosteriod, its concentration and the ointment base. the effect was still evident at extremely high dilutions of the steroids ... | 1975 | 1201192 |
| pure red cell aplasia: studies on an igg serum inhibitor neutralizing erythropoietin. | a new type of igg serum inhibitor in adult pure red cell aplasia (prca) has been investigated. this inhibitor is directed against circulating erythropoietin (ep) (prca type b), rather than the erythroid marrow (prca type a). thus, the igg inhibitor, after interaction with ep in solution, is precipitated together with ep by addition of goat anti-human gamma-globulins. pre-therapy prca serum, although apparently devoid of ep, shows considerable ep activity following acidification and boiling. the ... | 1975 | 1201224 |
| binding of vitamin b12--rat transcobalamin ii and free vitamin b12 to plasma membranes isolated from rat liver. | when dialysed rat serum which contains a single, low molecular weight binder for vitamin b12, rat transcobalamin ii (rat tc-ii), was labelled in vitro with 57co-vitamin b12 and then incubated at 30 degrees c (ph 7-5) with vesicles of highly purified plasma membranes separated from microsomal fractions of rat liver by density gradient centrifugation, the 57co-vitamin b12-rat tc-ii complex bound to high affinity sites on the vesicles via a specific (binding after correction for 'non-specific' bind ... | 1975 | 1201244 |
| ultrastructural studies of the bone marrow in sickle cell anaemia. ii. the morphology of erythropoietic cells and their response to deoxygenation in vitro. | electron microscopic studies of bone marrow aspirates obtained from patients with homozygous sickle cell anaemia (hbss) were fixed immediately without attempts to deoxygenate the samples. erythroblasts and normoblasts in these preparations were devoid of haemoglobin polymers or other indications of sickling. furthermore, the nucleated erythroid cells from sickle-cell patients presented an ultrastructural morphology indistinguishable from that of identically-processed erythroid cells in marrow sa ... | 1975 | 1201249 |
| comparative study of the smooth muscle contractor activity of airborne dusts and of dustiness in cotton, flax, and jute mills. | a bioassay technique using isolated guinea-pig ileum was employed to compare the smooth muscle contractor activity of various dusts from mills in which the prevalence of byssinosis was known. the activity of dust from a mill spinning a coarse grade of cotton was several times greater than that in dust from a mill processing a fine grade of cotton. there was a similar order in the difference of the prevalence of byssinosis in these mills. however, the activities of fine cotton, flax, and jute dus ... | 1975 | 1201255 |
| immunoglobulin synthesis and antibody content in the small intestine of the rabbit. | the concentration of immunoglobulin and specific antibody in the serum and the intestinal fluid and the rate of synthesis of immunoglobulin in the small intestine was measured in normal and immunized rabbits. iga was found to be the predominant immunoglobulin in the intestinal fluid. iga and igg were secreted at rates of 4-3 mug/cm/hr and 1-3 mug/cm/hr respectively. specific anti-vibrio cholerae antibodies in the intestine were found mainly in the iga class after oral immunization. | 1975 | 1204251 |
| pinocytic activity in neuraminidase treated ameba. | | 1975 | 1205516 |
| comparison of the rate of absorption and proteolysis of [14c]choleragen and [14c]bovine serum albumin in the rat jejunum. | [14c]choleragen was used to study the rate of disappearance of choleragen enterotoxin from the jejunum of rats. [14c]bovine serum albumin (bsa) was studied in a similar manner. almost one-third of the labeled toxin had disappeared from the intestine after 6 h. its rate of disappearance was the same in germfree rats as in conventional rats. the rate of proteolysis of [14c]choleragen and [14c]bsa by intestinal mucodal lysosomal enzymes was also studied. neither was significantly degraded by neutra ... | 1975 | 1205622 |
| rapid and reliable preparation of macroaggregated albumin suitable for lung scintigraphy. | | 1975 | 1205665 |
| protein reagent modification of cholera toxin: characterization of effects on antigenic, receptor-binding and toxic properties. | the effects of protein modification procedures on the biologically most important properties of cholera toxin, i.e. the toxic activity, the gm1 receptor-binding capacity and the antigenic (antibody-fixing) properties, have been studied quantitatively using microgram amounts or less of toxin protein. most of the 24 group-specific reagents used had either no inhibitory effect on the toxic or the combination of gm1-binding and antibody-fixing properties of cholera toxin, or they had a concomitant i ... | 1975 | 1206372 |
| growth inhibition of vibrio cholerae by d-camphor. | | 1975 | 1206376 |
| abortifacient effects of vibrio cholerae exo-enterotoxin and endotoxin in mice. | to study antifertility properties of microbial toxins, exoenterotoxin and endotoxin from vibrio cholerae were injected intravenously into mice at different times during pregnancy. the two substances induced termination of pregnancy, but the patterns of abortifacient activity were different. exotoxin terminated pregnancy in mice when administered between days 4 and 10 of gestation, but abortifacient activity was reduced in animals more than 10 days pregnant; exogenous progesterone did not protect ... | 1975 | 1206628 |
| [utilization of serological methods in the retrospective diagnosis of cholera and in detecting vibrion carriers]. | sensitivity and specificity of the three serological methods were studied comparatively: the vibriocidal test, the reaction of bacterial agglutination and of indirect hemagglutination, with the use of erythrocytes sensitized with the vibrio lyzate, cholera species o-antigen and cholerogen. investigations were conducted with the blood sera of cholera patients, vibrio carriers and contacts. vibriocidal test proved to be the most sensitive; its data correlated with the results of bacterial agglutin ... | 1975 | 1210898 |
| [role of neuraminidase from cholera vibrions in the pathogenesis of experimental cholera]. | a study was made of a possible effect of neuraminidase of cholera vibrios on cholera pathogenesis. it was shown that in intraintestinal injection of cholera vibrios of the el tor biotype to nursling rabbits neuraminidase could be revealed in their intestine 5 to 8 hours after the infection. addition of neuraminidase to the weakly cholerogenic strain cholera vibrios intensified its cholerogenic action in infection of the animals. the antineuraminidase serum administered to the infected rabbits pr ... | 1975 | 1210912 |
| a salmonella california contamination of a turkey feed concentrate. | | 1975 | 1212606 |
| selective chemical changes of gonadotropic hormones by means of neuraminidase from vibrio cholerae. | experiments indicate that neuraminidase from vibrio cholerae selectively cleaves sialic acid derivate from fsh, but leaves the lh sialic acid derivative. by performing a specific test for lh - the oaad test - we found that the lh activity is not influenced by the presence of neuraminidase. the fsh activity is destroyed by neuraminidase but the enzyme itself influences the ovarian weight, resulting in a weight decrease observed 48 hours after injection. before or after this "critical time" no dec ... | 1975 | 1212991 |
| [comparative study of the epidemiology of cholera and sensitivity to antibiotics of strains collected during the epidemiological period in 1971 and 1972 in wilaya de constantine]. | | 1975 | 1213515 |
| studies on the so-called nag vibrios. | | 1975 | 1213771 |
| studies on bacteriology of cholera infection in varanasi. | | 1975 | 1213804 |
| [changes in the composition of intracellular lipids and hydrocarbons in candida tropicalis in the absence of growth]. | the fatty acid composition of lipids and the composition of hydrocarbons were studied in candida tropicalis cultivated on a medium with propionic acid and incubated in the conditions of starvation and on a medium containing glucose-1-6-14c but no nitrogen. intracellular fatty acids with an odd number of carbon atoms were found to be easily metabolized by yeast cells. the content and composition of intracellular hydrocarbons were very labile and underwent intensive transformation caused by change ... | 1975 | 1214607 |
| [antimicrobic properties of facultative anaerobes isolated from oil-bearing wells]. | | 1975 | 1214635 |
| [formation of fatty acids by aspergillus ochraceus 15b growing on a hexadecane containing medium]. | | 1975 | 1214637 |
| [electron microscopic study of double infection induced by potato virus x and potato virus y in nicotiana glutinosa]. | | 1975 | 1214639 |
| [salmonellosis carrier state]. | | 1975 | 1214656 |
| [electron microscopy of cholera vibrios from the intestinal contents of infected suckling rabbits]. | electron microscopic study of the cells of classic, el tor and nag-vibrios showed them to be no different by the structure type from the cells of ofter gram-negative bacteria. a characteristic peculiarity of the cholera vibrios revealed after their passage through the intestine of nursling rabbits was the presence of microcapsules and protrusions of the areas of the wall membranous apparatus. | 1975 | 1217357 |
| recent studies on genetic recombination in vibrio cholerae. | | 1975 | 1219899 |
| interbiotype conversion of cholera vibrios by action of mutagens. | | 1975 | 1219900 |
| the antibody basis of local immunity to experimental cholera infection in the rabbit ileal loop. | | 1975 | 1219901 |
| bacteriology at the periphery of the cholera pandemic. | | 1975 | 1219904 |
| virulence-enhancing effect of ferric ammonium citrate on vibrio cholerae. | | 1975 | 1219909 |
| cell-wall antigens of v. cholerae and their implication in cholera immunity. | | 1975 | 1219915 |
| [ecg characteristics during its recording from electroanomalous points of the skin]. | | 1975 | 1223350 |
| the effects of cholera enterotoxin on intestinal tissue water as measured by nuclear magnetic resonance (nmr) spectroscopy. | cholera enterotoxin has been postulated to change the configuration of the intracellular protein-water system, altering the permeability of the cell to water. using nuclear magnetic resonance (nmr) spectroscopy, this protein-water relationship can be examined. small intestinal loops in the rat were injected with 0.5 ml of either schwarz/mann cholera enterotoxin (40 mug/cc saline solution) or normal saline. full thickness segments of intestine from each loop were taken and percentage water (using ... | 1975 | 1223921 |
| the anomeric configuration of n-acetylneuraminic acid released by the action of vibrio cholerae neuraminidase. | | 1975 | 1227974 |
| the in vitro activity of midecamycin against various vibrio species. | the susceptibility of various vibrio species to midecamycin was studied by the tube dilution method. a mic of 100 mug per ml of midecamycin in bhi broth was taken as an indicator of bacterial resistance. all vibrio cholerae classic biotype strains tested, were sensitive to midecamycin. in respectively, 87.4%, 60.7% and 89.5% of vibrio cholerae el tor biotype, vibrio parahaemolyticus and vibrio nag strains tested, were sensitive to midecamycin. | 1975 | 1228226 |
| [serological response in man to an anticholera vaccine made from killed vibrios and partially purified with aluminium hydroxide]. | the immunizing capability of a new anticholera vaccine (choleric anatoxin + vibrios ogawa and inaba) was tested on a group of 113 subjects. the vaccination was carried out with two inoculations of the vaccine, administered a month apart. testings were taken before the vaccination, before the second inoculation and 10 to 14 days after the vaccination was completed. the determination of the vibriocide antibodies was made following the test-tube method described by verwey and al., and the research ... | 1975 | 1230039 |
| [studies on the bacteriological diagnosis of cholera and on the characteristics of isolated strains in the apulia epidemic during the summer of 1973]. | descriptions are given of the characteristics of 107 strains of vibrio cholerae of the serum type ogawa isolated during the epidemic in 1973, which, although not exhibiting hemolysis in liquid culture, could be assigned because of their other characteristics to the el tor biotype and to the phagic type 1. the authors also make a number of technical annotations on the bacteriological diagnosis of cholera. | 1975 | 1230046 |
| [preliminary studies on anti-brucella agglutinins and on immunoglobulins in subjects affected by cholera during the 1973 epidemic in the province of bari]. | the behaviour of brucella agglutinins in sera from 70 cholera patients has been investigated. agglutinins for brucella, at titers of 1/40 or higher, occurred in 45 patients (64,2%); it has also been showed an antibody rise in 41 subjects with a peak titer during the second week, just like cholera agglutinins. this fact confirms the antigenic interrelationship between vibrio cholerae and brucella group. the serum immunoglobulins, during the course of infection, have been determined. mean levels o ... | 1975 | 1230047 |
| [studies on the spread of vibrio cholerae, biotype el tor in the environment during the period following the 1973 epidemic in apulia]. | in the period following the cholera epidemic, studies were made of sewage, seawater and various foodstuffs (green vegetables, mussels, etc.) in an attempt to isolate vibrio cholerae of the el tor biotype. only in the samples of sewage taken at bari and taranto was it possible to isolate vibrio cholerae biotype el tor for a period of about a month after the last case of the illness; in this period it was isolated in 5 of the 11 samples taken at bari and in 1 of the 14 taken at taranto. the positi ... | 1975 | 1230048 |
| genetic influence on saline consumption and salt hypertension as exhibited by the response of various rat strains and sub-strains. | female rats of 2 sprague-dawley sub-strains and of the wistar and fischer 344 strains were sensitized to the hypertensogenic effect of excess salt by removal of 1 kidney and by being given 1% nacl solution as their sole drinking fluid. one of the sprague-dawley sub-strains (spd) and the wistar (cfep) rats developed progressive saline polydipsia of equivalent degree, blood pressure rose, and about half of the members became hypertensive during treatment. rats of the other sprague-dawley strain (c ... | 1975 | 1231046 |
| [zoonoses in veterinary practice. 3. chlamydial, rickettsial and fungal infections]. | | 1975 | 1231047 |
| [induction of interphase chromosome condensation in cultured human lymphocytes]. | | 1975 | 1231100 |
| [a cytophotometric and karyometric study of the granular cells of the rat brain. i. granular cells of the cochlear complex and cerebellum]. | two cell types can be distinguished in granular cells of the chochlear complex of the rat by the form of their processes and the structure of the nuclear chromatin. it is shown cytophotometrically that the majority of the granular cells of the cochlear complex and in the cerebellar cortex are diploid. however, some cells contain dna amount close to tetraploid. some correlation between the size of the nucleus and its dna amount was discovered both in the cerebellar cortex and in the cochlear comp ... | 1975 | 1231101 |
| [characteristics of various nag vibriones isolated in italy]. | in this paper are reported the results of a comparative study of 4 "nag" vibrio strains isolated in italy and the vibrio biotype "e1 tor" responsible of the 1973 outbreak. the "nag" vibrios were practically identical with the "e1 tor" vibrio in biochemical properties, polypeptide composition, enteritogenic activity in rabbit ileal loops and showed various antigenic similarities in gel precipitation and indirect immunofluorescence tests. the possible pathogenic role of the "nag" vibrios is briefl ... | 1975 | 1231657 |
| [effect of different types of rations on various characteristics of ruminal and intermediary metabolism in sheep. 1. formation of volatile fatty acids]. | studies on ruminal physiology were made with 15 growing female merino sheep to investigate the influence of different types of rations on the fermentation of volatile fatty acids. the rations were constructed of green feed, straw and concentrates (type 1) or chemically treated straw pellets + concentrates (type 2). one ration (type 3) consisted of concentrates only. with regard to the total concentration of acids ration 3 was significantly superior to the 2 other types of rations. moreover, rati ... | 1975 | 1233967 |
| stimulation and inhibition of dna synthesis in rat thymocytes: action of concanavalin a and wheat germ agglutinin. | | 1975 | 1234047 |
| hbs-ag, hbc-ag and virus-like particles in liver tissue. | hepatitis b surface antigen (hbs-ag) and hepatitis b core antigen (hbc-ag) in hepatic tissue of 3 cases with various liver diseases were investigated by immunofluorescent method. virus-like particles were demonstrated by electron microscopy in the nuclei of these 3 cases. the localization of hbs-ag was restricted in cytoplasma or on the surface of hepatocyte, while hbc-ag was almost in the hepatocytic nuclei. however, there was unexplainable discrepancy between the distribution of hbs-ag and tha ... | 1975 | 1234098 |
| numerical analysis and computerized identification of the yeast genera candida and torulopsis. | numerical analysis of the published standard descriptions of 104 species of candida and 48 species of torulopsis suggested that the number of species should be reduced to 78 and 33 respectively. four examples are noted of closely matched species differing in ability to assimilate nitrate; these species may be combined as nitrate-variable species. although the ability to form pseudomycelium is the only important difference between the genera, only three examples were noted of candida and torulops ... | 1975 | 1236931 |
| cellular kinetics of the intestinal immune response to cholera toxoid in rats. | the aims of this study were (a) to find a regime of immunization with cholera toxoid in rats which would establish a high density of antitoxin containing cells (acc) in the lamina propria of the intestine and (b) to determine the origin of the acc. the best cellular response was achieved by a single i.p. dose of toxoid in fca followed by an intraintestinal boost 2 wk later. acc appeared in the thoracic duct lymph 2 days after boosting, reaching a peak of about 200,000 acc/h at 3--4 days. this wa ... | 1975 | 1238506 |
| survival in vibrio cholerae in the live shellfish. | | 1975 | 1239409 |
| [electron microscopic studies of epinephrine choroiditis in rabbits. 3. scanning electron microscopic observations of the retinal pigment epithelial cells in the early stage (author's transl)]. | | 1975 | 1239915 |
| [genetic characteristics of r factor of vibrio cholerae and adaptive development of resistance]. | | 1975 | 1240174 |
| [electron microscopic observation of cholera toxin molecules]. | | 1975 | 1240197 |
| [biological activity of the cell components of vibrio cholerae. ii. a comparison between if30 and i-lps fractions]. | | 1975 | 1240223 |
| [quantitative analysis of cholera toxin - use of cultured tumor cells and their mutants]. | | 1975 | 1240259 |
| [reverse transformation of tumor cells caused by cholera toxin and the substance inhibiting the process]. | | 1975 | 1240260 |
| [classification of vibrio cholerae and related organisms]. | | 1975 | 1240295 |
| [antibacterial immunity in experimental cholera]. | | 1975 | 1240315 |
| distribution of cholera organisms in experimental vibrio cholerae infections: proposed mechanisms of pathogenesis and antibacterial immunity. | this study was undertaken to determine the sequence of events in the microenvironment of the intestinal tract that culminate in the symptoms of cholera and to attempt to define more clearly the mechanisms involved in antibacterial immunity. the extent to which mucus occurs in the normal intestine of rabbits and the appearance of the intestinal villi in unfixed frozen sections was demonstrated. the villi and intervillous spaces were found to be normally covered by a layer of mucoid material that ... | 1976 | 1248870 |
| familial clustering of selective iga deficiency. | serum iga levels of 35 healthy iga-deficient index cases, of their 180 first-degree relatives, and of 31 other family members were studied. iga deficiency was detected in 7.2% of the first-degree relatives, which is significantly more than the 0.25% frequency of iga deficiency in healthy finnish blood donors. subnormal serum iga levels were found in additional 13 (7.2%) first-degree relatives. the familial clustering of iga deficiency seemed to be controlled by multigenic factors. iga-deficient ... | 1976 | 1251582 |
| chemical characterization of the structure of cholera toxin and its natural toxoid. | acrylamide gel electrophoresis demonstrated that the toxin of vibrio cholerae is comprised of three polypeptide chains, alpha, beta, and gamma, of molecular weights 24,000, 9,700, and 9,700 daltons, respectively. amino acid sequence analysis of intact toxin indicated a molecular composition of alpha gamma beta4. acrylamide gel electrophoresis and sequence analysis confirmed that the natural toxoid (choleragenoid) is identical to the toxin beta-chain. the alpha- and gamma-chains of the toxin are ... | 1976 | 1254995 |
| aspects of the interaction of vibrio cholerae toxin with the pigeon red cell membrane. | the ganglioside galactosy-n-acetylgalactosaminyl [sialosyl] lactosyl ceramide (gm1) is readily accumulated in pigeon red cell membranes soaked with [3h]gm1 (1-100 mug/ml) at 37 c for 30 min. this treatment enhances the activation of adenyl cyclase by the toxin of vibrio cholerae. an attempt was made at correlation of the amount of incorporated gm1 with the increased binding of toxin and activation of adenyl cyclase. cells with less than 2 mug of incorporated gm1 per 4 x 10(9) cells bind 5-10 mug ... | 1976 | 1254996 |
| stimulation of adenylate cyclase by vibrio cholerae toxin and its active subunit. | the mechanism by which vibrio cholerae toxin and its active subunit a stimulate adenylate cyclase of rat liver plasma membrane after in vivo or in vitro exposure was investigated. stimulation of adenylate cyclase was more efficient with cholera toxin than with subunit a in whole animals and in "soluble" preparations of adenylate cyclase. toxin-stimulated plasma membrane adenylate cyclase activity persisted after solubilization from the membrane by detergent, while subunit a stimulation was destr ... | 1976 | 1254997 |
| antitumor immunity. ii. viability, tumorigenicity, and immunogenicity of neuraminidase-treated tumor cells: effective immunization of animals with a tumor vaccine. | a dimethylbenzdithionaphthene (dbdn)-induced fibrosarcoma showed reduced transplantability if previously treated with vibrio cholerae neuraminidase (vcn). however, the reduced transplantability of vcn-treated tumor cells was not associated with any loss of their viability or tumorigenic capability, but appeared to be due to their increase in immunogenicity. high doses of vcn-treated tumor cells could grow even in normal individuals. lower doses, which did not induce tumor development in normal i ... | 1976 | 1255753 |
| selective modification of cell surface proteins and thymidine transport in hamster cells exposed to cholera toxin. | the increased adherence and morphological response which occurs in chinese hamster ovary cells as a result of exposure to cholera toxin is paralleled by modification in the relative exposure of outer proteins. mild proteolysis treatment of the cells prelabeled with [3h] glucosamine reveals a markedly different kinetics of release of external glycopeptides as a result of exposure to cholera toxin. selective alterations in external tyrosyl-rich proteins can also be detected by lactoperoxidase-cata ... | 1976 | 1256059 |
| [exocrine pancreatic function, intestinal resorption and reaction to vibrio cholerae infection in protein deficiency]. | six patas monkeys were fed a protein-free diet while 16 animals of the same species receoved a standard monkey diet. the protein-depleted monkeys showed reduced absorption of folic acid but not dxylose, and 5 out of 6 exhibited depressed exocrine pancreatic function. when challenged with vibrio cholerae, animals with reduced folic acid absorption and pancreatic enzyme production excreted the micro-organisms for a longer time. | 1976 | 1257727 |
| interactions of cadmium and selenium in rat plasma in vivo and in vitro. | 75se and 109cd tracers were used to study the binding of se and cd to plasma proteins at various seo32- doses and times upt to 24 h after the simultaneous subcutaneous administration of seo32- markedly increased both se and cd plasma levels over that in control animals. gel permeation chromatography of plasma indicated that at all times up to 24 h cd and se were bound in an atomic ratio of approx. 1 : 1 in 330 000 and 130 000 dalton fractions. from 4 to 24 h, cd and se appeared in the 420 000 da ... | 1976 | 1260013 |
| virulence factors involved in the intraperitoneal infection of adult mice with vibrio cholerae. | nonmotile mutants of vibrio cholerae isolated from ogawa, inaba, and el tor strains were less virulent than parent wild types when administered to adult mice intraperitoneally. the cells were suspended in 5% hog gastric mucin. antitoxic immunity did not protect mice against this type of challenge, but a ribosomally derived vaccine did. intraperitoneal injection of 10 50% lethal doses of enterotoxin (based on intravenous doses) was without toxic manifestations as were 10(10) heat-killed vibrios s ... | 1976 | 1262061 |
| the detection of antibodies in human and animal filariases by counterimmunoelectrophoresis with dirofilaria immitis antigens. | counterimmunoelectrophoresis revealed the presence of precipitin antibody in all of 6 dogs and the 1 cat infected with dirofilaria immitis and in the serum of 17 of 24 individuals living in a setting of hyperendemic subperiodic bancroftian filariasis. antigens used in the test were prepared from microfilariae and adult male d. immitis. some humans and animals had antibodies to both antigens while others had antibodies against microfilariae or adult worms only. the presence of soluble circulating ... | 1976 | 1262695 |
| attenuated, streptomycin-dependent salmonella typhi oral vaccine: potential deleterious effects of lyophilization. | four studies were done with streptomycin-dependent salmonella typhi as an oral, attenuated vaccine. studies 1 and 3 employed freshly harvested vaccine, whereas studies 2 and 4 involved lyophilized vaccine. five to eight doses (3 x 10(10)-10(11) organisms/dose) were given; oral streptomycin (1.0 g) was administered concomitantly in studies 2 and 3, with only two of the doses of vaccine in study 1, and was not given in study 4. no adverse reactions were encountered in 179 vaccinated men, and 94% o ... | 1976 | 1262709 |
| correspondence: growth plate defects in foals. | | 1976 | 1265980 |
| biochemical studies on the cell wall lipopolysaccharides (o-antigens) of vibrio cholerae 569 b (inaba) and el-tor (inaba). | lipopolysaccharides were isolated from the cell walls of vibrio cholerae 569 b (inaba) and el-tor (inaba). chemical analysis revealed the presence of glucose, fructose, mannose, heptose, rhamnose, ethanolamine, fatty acids and glucosamine. the lipopolysaccharides do not contain 2-keto-3-deoxyoctonate, the typical linking sugar of polysaccharide and lipid moieties of enterobacterial lipopolysaccharides. galactose, a typical core polysaccharide component of many gram-negative bacteria was also abs ... | 1976 | 1268236 |
| synergistic protective effect in rabbits of immunization with vibrio cholerae lipopolysaccharide and toxin/toxoid. | subcutaneous immunization of rabbits with a combination of vibrio cholerae lipopolysaccharide (lps) and enterotoxin induced a more than 100-fold-higher degree of protection against intestinal challenge with live cholera vibrios than did vaccination with either of the two antigens alone. such a synergistic effect was also obtained by immunization with a combination of lps and choleragenoid. the immunization with lps and toxin (or toxoid) in combination did not enhance the reistance to toxin chall ... | 1976 | 1270131 |
| morphological and steroidogenic changes in cultured adrenal tumor cells induced by a subunit of cholera enterotoxin. | a purified subunit of the cholera enterotoxin molecule was found to have morphological and steroidogenic inducing effects similar to those induced by the native enterotoxin on monolayer tissue cultures of y1 adrenal tumor cells, although 1,000 times more subunit than toxin (weight basis) was required for maximal effects. in contrast to the whole toxin, the effects of the active subunit could not be prevented by prior incubation with either gm1 ganglioside or with antibodies directed against chol ... | 1976 | 1270152 |
| colony morphology of vibrio cholerae on sv medium. | | 1976 | 1270369 |
| effect of hgcl2 on rat kidney cells in primary culture. | single cell suspensions, obtained from rat kidneys, were cultured with various concentrations of hgcl2 for different times to examine the effects on cellular synthetic activity. unexpectedly, the rna synthesis of kidney cells was stimulated with low doses of hgcl2, whereas it was inhibited with higher doses. even at a higher dose, rna synthesis was stimulated during early incubation but was inhibited during longer incubations. thus, the effect of mercury showed a biphasic pattern both in dose-re ... | 1976 | 1271750 |
| effect of hgcl2 on rat kidney cells in primary culture. | single cell suspensions, obtained from rat kidneys, were cultured with various concentrations of hgcl2 for different times to examine the effects on cellular synthetic activity. unexpectedly, the rna synthesis of kidney cells was stimulated with low doses of hgcl2, whereas it was inhibited with higher doses. even at a higher dose, rna synthesis was stimulated during early incubation but was inhibited during longer incubations. thus, the effect of mercury showed a biphasic pattern both in dose-re ... | 1976 | 1271750 |
| modified tumor cells in the immunotherapy of solid mammary tumors. | | 1976 | 1271892 |
| teratogenic potential of noise in mice and rats. | | 1976 | 1273844 |
| acetylcholine release from the nerve terminals of the guinea-pig ileum by naja venoms. | | 1976 | 1273858 |
| [test of degranulation of basophils and mast cells for detection of allergic diseases (literature survey)]. | | 1976 | 1274295 |
| sequence similarity between cholera toxin and glycoprotein hormones: implications for structure activity relationship and mechanism of action. | | 1976 | 1275921 |
| proceedings: increase in prostaglandin e2 and f2alpha in inflammatory exudate at 4-48 h after uvb irradiation of human skin. | | 1976 | 1276048 |
| [reduction of the biological activity of vibrio cholerae culture filtrates exposed to neuraminidase inhibitors]. | experiments were conducted on a model of edema of albino mouse paws; a study was made of the effect of neuraminidase inhibitors on the cholerogenic effect of a filtrate of cholera vibrio culture. it appeared that addition to the filtrate of inhibitors depressed its biological activity. since no cholerogenic action was possessed by the purified neuraminidase preparations from the cholera vibrios it was suggested that there existed a chemical affinity between the region of cholerogen responsible f ... | 1976 | 1276448 |