| intestinal bacterial flora and host defense mechanisms. | the protective mechanisms of intestinal bacterial flora against exogenous infections will be discussed in this paper. experimental data on protective function of escherichia coli, enterococcus faecalis and bacteroides distasonis comprising intestinal flora against oral infection of shigella flexneri which causes localized infection are presented. furthermore, the present investigation deals with non-specific defense mechanism which indicate that protective activity of intestinal flora against pa ... | 1986 | 2968005 |
| genetics and regulation of enterobactin genes in shigella flexneri. | although shigella flexneri possesses the genes for two siderophore systems, enterobactin and aerobactin, the enterobactin system is only rarely utilized. to investigate the regulation of enterobactin expression in s. flexneri, all of the genes specifically required for synthesis and transport of enterobactin were cloned from both an expressing (ent+) and a nonexpressing (ent-) strain. notable differences between the cloned genes included endonuclease restriction site changes and the presence of ... | 1988 | 2973458 |
| [clinical characteristics of dysentery today]. | | 1988 | 2973539 |
| myosin atpase activity during avian cardiac and skeletal muscle development. | the myosin atpase activity and myosin light chain composition in developing chick heart and skeletal muscles were studied and compared. embryonic myosin was purified and characterized from day 7 to day 19 of embryogenesis. embryonic cardiac myosin generally showed the same ca2+-activated myosin atpase activity level as the adult value. in comparison, pooled pectoralis and hindlimb skeletal muscles from day 10 through day 19 showed myosin atpase activities that were all significantly less than th ... | 1988 | 2976104 |
| apparent hyperalgesia in the mouse tail-flick test due to increased tail skin temperature after lesioning of serotonergic pathways. | the relationship between tail skin temperature and responsiveness to noxious radiant heat in the tail-flick test was investigated in mice. a significant negative correlation between tail skin temperature and tail-flick latency was found when the tail skin temperature was increased by elevating the ambient temperature. after intracerebroventricular injection of the serotonin neurotoxin 5,7-dihydroxytryptamine (5,7-dht, 80 micrograms) tail skin temperatures were increased and tail-flick latencies ... | 1988 | 2976242 |
| [rpo pathway as a possible recombination mechanism of the interaction between dna of transducing phages and the escherichia coli genome]. | the conditions affecting recombination of dnas of transducing lambdoid rifd phages with the chromosome of escherichia coli k-12 reca in the region of homology were studied. in support to the previously obtained data, the int system was shown to take no part in the process. the homologous character of recombination interactions and their dependence on efficiency of transcription were demonstrated. it is therefore suggested that recombination takes the rpo pathway. similar peculiarities were revea ... | 1988 | 2976700 |
| hypersensitivity to shigella outer membrane proteins in mice. | mice sensitized with living sh. flexneri 3a or with outer membrane proteins (omp) derived from these bacteria showed arthus and delayed type hypersensitivity (dth) when injected in the footpad with omp. the delayed reactivity to omp was transferable to normal mice by spleen cells obtained from donor animals which were previously sensitized with living bacteria. | 1987 | 2977277 |
| [the possibility of using the demonstration of shigella antigens in the study of the epidemic process in dysentery]. | the detection of shigella antigens in feces in accordance with the results of the passive hemagglutination test is more effective than the bacteriological method for finding out persons infected with shigella, especially in the absence of pronounced clinical manifestations of dysentery, and can be used in the study of the mechanism of the epidemic process. the examination of two groups, each consisting of permanent and temporary members, has revealed that the use of this method for detection of ... | 1988 | 2977464 |
| [the results of a seroepidemiologic study of newcastle dysentery]. | differences in the monthly distribution in the number of seropositive individuals among children and adults in years with different intensity of the epidemic process have been revealed. immunity in cases of dysentery caused by s. newcastle reflects the yearly and seasonal activation of the epidemic process in this shigella infection both in children and adults, the seroconversion characteristics observed in the year of a high morbidity level being a more objective criterion indicating the beginn ... | 1988 | 2977465 |
| [experimental study of atherosclerosis. i. anti-atherosclerotic actions of chinese herb effective component 8501]. | | 1988 | 2978250 |
| characterization of a major protein with a molecular weight of 160,000 associated with the viral capsid of epstein-barr virus. | a monoclonal antibody designated v3 was produced against a late protein associated with the epstein-barr virus-induced viral capsid antigen complex. the antibody reacted with discrete patches in the nuclei of infected cells as well as with virus particles, as shown by immunofluorescence and ultrastructural immunoperoxidase staining. the molecular weight of the protein precipitated by this monoclonal antibody was ca. 160,000. all anti-viral capsid antigen antibody-positive sera tested in an enzym ... | 1985 | 2981328 |
| middle tumor antigen of polyomavirus transformation-defective mutant ng59 is associated with pp60c-src. | we have found that lysis of mouse embryo cells infected with the polyomavirus host range transformation-defective (hr-t) mutant ng59 under gentle conditions that avoid ionic detergents results in detectable ng59-encoded middle tumor antigen (mtag) associated with pp60c-src. this mtag-pp60c-src complex could be immunoprecipitated from ng59-infected cell lysates by either sera from animals bearing polyomavirus-induced tumors or by monoclonal antibodies directed against mtag. immune complex kinase ... | 1985 | 2981329 |
| genome organization of herpesvirus aotus type 2. | herpesvirus aotus type 2, a virus commonly found in owl monkeys without overt disease, has a similar genome structure to the oncogenic herpesviruses of nonhuman primates (herpesvirus saimiri, herpesvirus ateles). virion dna of herpesvirus aotus type 2 (m-dna) has an unique 110-kilobase-pair region of low g + c content (40.2%, l-dna), inserted between stretches of repetitive h-dna (68.7% g + c, about 41 kilobase pairs per molecule) that are variable in length. a minority of virions contain defect ... | 1985 | 2981331 |
| autoregulation of the bacteriophage p22 scaffolding protein gene. | during the formation of each bacteriophage p22 head, about 250 molecules of the product of gene 8, scaffolding protein, coassemble with and dictate correct assembly of the coat protein into a proper shell structure. at approximately the time that dna is inserted inside the coat protein shell, all of the scaffolding protein molecules leave the structure. they remain active and participate in several subsequent rounds of shell assembly. previous work has shown that scaffolding protein gene express ... | 1985 | 2981337 |
| analysis of mobilization elements in plasmids from shigella flexneri. | the mobilization properties of three plasmids were examined after cotransfer from shigella flexneri to escherichia coli. the largest plasmid, pcn1, was shown to be a conjugative r factor that could promote its own transfer and allow cotransfer of a 4.1-kilobase plasmid, pcn3; mobilization of the third plasmid, pcn2 (6.3 kilobases), required the presence of both pcn1 and pcn3. sequences from pcn2 and pcn3 homologous to the bom (basis of mobilization) sites of cole1 and pbr322 were localized by an ... | 1985 | 2982788 |
| adenine nucleotides directly stimulate pertussis toxin. | both cholera toxin and pertussis toxin catalyzed adp-ribosylation of purified bovine brain tubulin. the effect of cholera toxin was evident in the absence or presence of nucleotides. in contrast, pertussis toxin required adenine nucleotides for its adp-ribosylating activity. atp, atp gamma s, app(nh)p, deoxy-atp, and adp all supported pertussis toxin-catalyzed adp-ribosylations in the absence or presence of edta, suggesting that nucleotide hydrolysis was not involved. adenine nucleotides also pr ... | 1985 | 2982826 |
| a new beta-lactamase plasmid in neisseria gonorrhoeae. | | 1985 | 2984155 |
| escherichia coli 23s ribosomal rna truncated at its 5' terminus. | in a strain of e. coli deficient in rnase iii (abl1), 23s rrna has been shown to be present in incompletely processed form with extra nucleotides at both the 5' and 3' ends (king et al., 1984, proc. natl. acad. sci. u.s. 81, 185-188). rna molecules with four different termini at the 5' end are observed in vivo, and are all found in polysomes. the shortest of these ("c3") is four nucleotides shorter than the accepted mature terminus. in growing cells of both wild-type and mutant strains up to 10% ... | 1985 | 2987818 |
| opioid modulation of ingestive behaviors in woodchucks and racoons. | we have examined the effect on feeding of opioid blockade with naloxone in two species which demonstrate a marked seasonality in their feeding patterns, the racoon (procyon lotor) and the woodchuck (marmota monax). naloxone suppressed food intake in the woodchuck which is a true hibernator. naloxone failed to suppress food intake in the racoon and, in fact, enhanced intake of a preferred sucrose solution. in the racoon, ir-dynorphine concentrations were extremely high in the hypothalamus compare ... | 1985 | 2987986 |
| isolation of retinoic acid-resistant clones from human breast cancer cell line mcf-7 with altered activity of cellular retinoic acid-binding protein. | after ethyl methane sulfonate mutagenesis of the mammary carcinoma cell line, mcf-7, we have isolated three clones, u-2, u-3, and u-9, resistant to retinoic acid. these three clones showed more than a 1000-fold higher level of resistance to retinoic acid than the parental mcf-7 cells when assayed by colony formation in monolayer culture system or by growth curves. the three resistant clones showed a 200-fold higher resistance to 13-cis-retinoic acid, about 10-fold higher resistance to retinol, a ... | 1985 | 2988768 |
| mercuric reductase structural genes from plasmid r100 and transposon tn501: functional domains of the enzyme. | the nucleotide sequence for the 2240 bp of plasmid r100 following the merc gene of the mercuric resistance operon has been determined and compared with the homologous sequence of transposon tn501. the sequences following merc and preceding the next structural gene mera are unrelated between r100 and tn501 and differ in length, with 72 bp in tn501 and 509 bp in r100. the r100 sequence has a potential open reading frame (orf) for a 140 amino acid polypeptide with a reasonable translational start s ... | 1985 | 2989109 |
| cloning of plasmid dna sequences involved in invasion of hela cells by shigella flexneri. | a large plasmid is found in virulent isolates of shigella sp. and encodes functions essential for invasion of mammalian cells. to identify plasmid sequences necessary for invasion, we isolated a series of tn5 insertions in pwr100, the virulence plasmid of shigella flexneri serotype 5. these insertions demonstrated that three separate ecori fragments of pwr100 were required for invasion of hela cells. however, the corresponding native ecori fragments, when cloned into pbr325, did not restore viru ... | 1985 | 2989179 |
| inhibition and activation of bovine heart nad-specific isocitrate dehydrogenase by atp. | in the absence of added calcium, inhibition of nad-specific isocitrate dehydrogenase by atp occurred without adp (i0.5 = 1.8 mm) and with 0.2 mm adp3- (i0.5 = 1.0 mm) at subsaturating substrate concentrations at ph 7.4. inhibition by atp was competitive with nad+ in the presence and absence of adp and was not reversed by magnesium citrate. no reversal of atp inhibition by free ca2+ was observed in the presence of adp (0.2 mm). however, when adp was absent, increasing ca2+ first caused progressiv ... | 1985 | 2990340 |
| bowenoid papulosis. presence of human papillomavirus (hpv) structural antigens and of hpv 16-related dna sequences. | this study reviews 39 cases of anogenital bowenoid papulosis lesions in 22 individuals of both sexes that were analyzed clinically, histologically, immunocytochemically, and virologically. macroscopically, three different types of lesions were demonstrated: erythematous macules; papules (lichenoid and/or pigmented papules); and leukoplakialike lesions. microscopically, bowenoid papulosis fulfills the criteria of a squamous cell carcinoma in situ. much like oral precancers, three distinct growth ... | 1985 | 2990353 |
| [survival of aujeszky's disease virus in infected pig slurry]. | it has been demonstrated that after experimental infection of pig slurry from the space under the slatted floor (infection dose of 10(6)pfu per ml), the aujeszky's disease virus (adv) survived for 72 hours at the temperature of 15 degrees c and at ph 6.5, but was inactivated after 96 hours. when technologically treated pig slurry from the storage tanks was saturated with water and infected with adv at the dose of 10(5)pfu per ml, the virus survived for 23 days when kept at 15 degrees c and 4 deg ... | 1985 | 2992149 |
| rates of cholesterol synthesis and low-density lipoprotein uptake in the adrenal glands of the rat, hamster and rabbit in vivo. | the absolute rate of cholesterol acquisition from de novo synthesis and from receptor-dependent and receptor-independent low-density lipoprotein (ldl) uptake was determined in the adrenal glands of the rat, hamster and rabbit under in vivo conditions. the rate of incorporation of [3h]water into cholesterol in the adrenal gland was much higher in the hamster (1727 nmol/h per g) and rabbit (853 nmol/h per g) than in the rat (71 nmol/h per g). assuming that 23 atoms of 3h are incorporated into the ... | 1985 | 2992599 |
| suicide vector for transposon mutagenesis in pseudomonas solanacearum. | a suicide vector was constructed by cloning the transfer genes of the wide-host-range (incw group) plasmid r388 into the bamhi site of pbr325. this plasmid can deliver tn5 into pseudomonas solanacearum at frequencies ranging from 10(-6) to 10(-9) per recipient. | 1985 | 2993241 |
| amino acid sequence of acylphosphatase from porcine skeletal muscle. | the amino acid sequence of acylphosphatase from porcine skeletal muscle was determined. it consists of 98 amino acid residues with n-acetylserine at the amino (n)-terminus: ac-ser-thr-ala-arg-pro-leu-lys-ser-val-asp-tyr-glu-val-phe-gly -arg-val-gln-gly-val-cys-phe-arg-met-tyr-thr-glu-asp-glu-ala-arg-lys-ile -gly-val-val-gly-trp-val-lys-asn-thr-ser-lys-gly-thr-val-thr-gly-gln -val-gln-gly-pro-glu-glu-lys-val-asn-ser-met-lys-ser-trp-leu-ser-lys -ile-gly-ser-pro-ser-ser-arg-ile-asp-arg-thr-asn-phe- ... | 1985 | 2993259 |
| the biologic significance of the mixed lymphocyte kidney culture in humans. | the mixed lymphocyte kidney culture (mlkc) in humans has been studied in normal and abnormal clinical conditions. human renal cortical cells were extracted by collagenase treatment from the kidneys of "normal" heart-beating cadaver organ donors (n = 13), patients with end-stage renal disease (esrd) at pretransplant bilateral nephrectomy and splenectomy (n = 13), and from irreversibly rejected renal allografts at the time of graft nephrectomy (n = 5). proliferation of peripheral blood t lymphocyt ... | 1985 | 2996186 |
| [the virus causing human t-cell lymphomas-leukemias]. | | 1985 | 2996236 |
| the e7-associated cell-surface antigen: a marker for the 11p13 chromosomal deletion associated with aniridia-wilms tumor. | unbalanced interstitial deletions of the p13 region of human chromosome 11 have been associated with congenital hypoplasia or aplasia of the iris, mental retardation, ambiguous genitalia, and predisposition to wilms tumor of the kidney. utilizing somatic cell hybrids containing either the normal or abnormal chromosome 11 from a child with wilms tumor and aniridia, we previously mapped the e7 cell-surface antigen to the 11p1300-to-11p15.1 region. to localize even further the site of this antigen ... | 1985 | 2996335 |
| acquired immune deficiency syndrome. a perspective for the medical practitioner. | aids is a new disease process with complications and management problems unlike anything ever seen before. an attempt has been made to summarize the available information about its various clinical presentations and how to manage them. subjects covered will include associated malignancies, infections, transmissibility, prodromal states, and infection control issues. | 1985 | 2997554 |
| pyrimidinones. 1. 2-amino-5-halo-6-aryl-4(3h)-pyrimidinones. interferon-inducing antiviral agents. | interferon induction and antiviral activity was discovered with 2-amino-5-bromo-6-phenyl-4(3h)-pyrimidinone. an analogue study incorporating a series of 2-amino-5-substituted-6-arylpyrimidinones revealed that the most potent interferon inducers were mono- and difluorophenyl analogues. these same analogues were also potent antiviral agents against semliki forest virus and herpes simplex type 1. in addition the monomethoxyphenyl analogues were potent antiviral agents but weak interferon inducers. ... | 1985 | 2999405 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| elicitation of conspecific attack or defense in the male rat by intraventricular injection of a gaba agonist or antagonist. | the involvement of central gabaergic mechanisms in the control over offensive and defensive behaviours in the rat was studied using intracerebroventricular injections (5 microliter) of a gaba agonist (thip) or a gaba antagonist (bicuculline methiodide). intracerebroventricular injections of thip (1.25 and 2.5 micrograms) induced attacks and offensive sideways towards an untreated partner, in animals placed in a neutral area where no aggressive reactions occur in controls. social approach behavio ... | 1985 | 2999844 |
| role of leukotrienes in rat reversed passive arthus pleurisy and the effect of aa-861, a 5-lipoxygenase inhibitor. | in studies of the role of leukotrienes in inflammatory reactions, the induction of rat reversed passive arthus pleurisy (a type iii allergic reaction) was employed. increases of exudate volume, vascular permeability, and migration of inflammatory cells in the pleural cavity were observed. the vascular permeability was enhanced biphasically during 0-30 min (early response) and during 3-6 h (late response) after induction of the pleurisy. the infiltration of inflammatory cells, mainly polymorphonu ... | 1986 | 3000949 |
| flaviviridae. | the family flaviviridae comprises the genus flavivirus, which contains 65 related species and two possible members. they are small, enveloped rna viruses (diameter 45 nm) with peplomers comprising a single glycoprotein e. other structural proteins are designated c (core) and m (membrane-like). the single strand of rna is infectious and has a molecular weight of about 4 x 10(6) and an m7g 'cap' at the 5' end but no poly(a) tract at the 3' end; it functions as the sole messenger. the gene sequence ... | 1985 | 3000978 |
| repression of adenovirus early gene expression by coinfection with a temperature-sensitive mutant in the immediate-early gene of pseudorabies virus. | wild-type adenovirus was coinfected with a mutant temperature sensitive for the immediate-early gene of pseudorabies virus. at the nonpermissive temperature, this mutant, tsg, strongly inhibited the transcription of all adenovirus early genes, including e1a. this inhibition was not observed with wild-type pseudorabies virus coinfection or with tsg coinfection at the permissive temperature. the level of repression was dependent upon the ratio of tsg to adenovirus in the infection. the results sug ... | 1986 | 3001339 |
| relationship of acth1-39-immunostained fibers and magnocellular neurons in the paraventricular nucleus of rat hypothalamus. | dual antigen immunocytochemical staining procedures were used in the same tissue section to determine the distribution of acth immunostained fibers and varicosities within the magnocellular and parvocellular divisions in the paraventricular nucleus (pvn) of rat hypothalamus and elucidate its anatomical relationship to vasopressin (vp) and oxytocin (oxy)-containing neurons. double immunostained preparations using glucose oxidase-antiglucose oxidase complex combined with pap complex to visualize t ... | 1985 | 3001668 |
| characterization of electron transfer and proton translocation activities in trypsin-treated bovine heart mitochondrial cytochrome c oxidase. | bovine heart mitochondrial cytochrome c oxidase has been treated with trypsin in order to investigate the role of components a, b, and c (nomenclature of capaldi) in cytochrome c binding, electron transfer, and proton-pumping activities. cytochrome c oxidase was dispersed in nondenaturing detergent solution (b. ludwig, n. w. downer, and r. a. capaldi (1979) biochemistry 18, 1401) and treated with trypsin. this treatment inhibited electron transfer activity by 9% when compared to a similarly trea ... | 1985 | 3002279 |
| molecular alteration of the 140-megadalton plasmid associated with loss of virulence and congo red binding activity in shigella flexneri. | a plasmid of about 140 megadaltons has been associated with the invasiveness of shigella flexneri. upon subculturing in liquid media of fully virulent isolates of shigella flexneri 2a ysh6000, which contains only a 230-kilobase-pair (kbp) plasmid in addition to 3.3- and 4.2-kbp cryptic plasmids characteristic to all s. flexneri strains, loss of invasiveness, loss of congo red binding activity (pcr), and complete loss of, or a deletion, or even a single-site is insertion in the plasmid occurred s ... | 1986 | 3002985 |
| bronchiolar and alveolar lesions in the pathogenesis of crocidolite-induced pulmonary fibrosis in mice. | asbestosis is generally considered to result in restrictive pulmonary disease associated with interstitial fibrosis. recently, however, attention has focused upon bronchiolar lesions and concomitant obstruction to air flow. the responses of conducting airways and alveoli were studied over a 20 week period following instillation of crocidolite to mice. the location of the lesions and the sequential inflammatory changes were studied by bronchoalveolar lavage, light and electron microscopy; regener ... | 1985 | 3003309 |
| [the kallikrein-kinin system in acute peritonitis and methods of correcting its disorders]. | in experimental fecal peritonitis in 140 albino rats the kallikrein-kinin system was shown to undergo the following changes: its inhibition during the first day was followed by an activation on the second day and inanition on the third day. under conditions of hyperbaric oxygenation the normalizing effect on the kinin system was observed during two days. the application of salicylates against the background of hbo was shown to enhance the normalizing effect which lasted for three days. | 1985 | 3004000 |
| fluctuation of specific iga antibodies in human milk. | the concentration of secretory iga and the levels of iga specific antibodies against escherichia coli labile-toxin, shigella flexneri 6, and rotaviruses were determined in milk samples obtained serially from women during the first 16 weeks postpartum. the mean concentration of secretory iga followed the expected pattern; the levels of specific antibodies fluctuated in an unpredictable manner and independently of milk secretory iga content, becoming undetectable in many instances. under some circ ... | 1985 | 3004100 |
| hormonal control of polyamine levels in bovine adrenocortical cells. | the implication of polyamines in cellular growth and differentiation processes and the existence of a polyamine-mediated protein phosphorylation system in adrenocortical cells suggest that polyamines may be examined as potential intracellular messengers in the pleiotypic action of acth. bovine adrenocortical cells in culture exhibit a specific, energy-dependent, partly sodium-supported, inward polyamine transport system, independent of the a, l, and n aminoacid uptake systems. steroidogenic conc ... | 1986 | 3004891 |
| studies on the glycoprotein nature of the thyrotropin receptor: interaction with lectins and purification of the bovine protein with the use of bandeiraea (griffonia) simplicifolia i affinity chromatography. | the tsh receptor from triton-solubilized bovine microsomal membranes was found to bind to a substantial extent to columns of the immobilized lectins bandeiraea (griffonia) simplicifolia i, ricinus communis i, wheat germ, and concanavalin a, whereas it was not retained by dolichos biflorus. elution of tsh receptor activity from these lectins could be achieved with the appropriate saccharides in all cases except concanavalin a. the most extensive adsorption of the receptor occurred on b. simplicif ... | 1986 | 3004903 |
| [similar organization of beta,beta'-subunit rna-polymerase genes and adjacent ribosomal protein genes in enterobacteriaceae and pseudomonas putida]. | the genes coding for the rna-polymerase beta,beta'-subunits and adjacent ribosomal protein genes in escherichia coli, salmonella typhimurium, shigella flexneri, serratia marcescens, proteus mirabilis and pseudomonas putida are compared by the southern hybridization procedure. in all the species studied close clustering of the genes rplkajl and rpobc is demonstrated. preliminary physical maps for these genes in s. typhimurium, s. flexneri, s. marcescens and p. mirabilis are proposed. rifampicin i ... | 1986 | 3005845 |
| enteropathogens associated with acute diarrhea in hospitalized infants. | thirty-five infants of low socioeconomic status who were living in urban santiago were hospitalized for acute diarrhea were prospectively evaluated for the presence of enteropathogens associated with the episode. some degree of malnutrition was evident in 20 infants (57.1%); 15 of these (75%) were under 6 months of age. mean duration of the hospital stay was 11.8 days for well-nourished patients and 15.7 days for the malnourished patients. one or more enteropathogens were found in 60% of the cas ... | 1986 | 3007718 |
| complete dissociation between the activation of phosphoinositide turnover and of nadph oxidase by formyl-methionyl-leucyl-phenylalanine in human neutrophils depleted of ca2+ and primed by subthreshold doses of phorbol 12,myristate 13,acetate. | evidences have been provided by many laboratories that the activation of the nadph oxidase in neutrophils by formyl-methionyl-leucyl-phenylalanine (fmlp) is strictly linked to a transduction pathway that involves the stimulation, via gtp binding protein, of the phosphoinositide turnover and the increase in [ca2+]i. the results presented in this paper demonstrate that fmlp can activate the nadph oxidase by triggering a transduction pathway completely independent of phosphoinositide turnover and c ... | 1986 | 3008746 |
| identification by antibody to a synthetic peptide of a protein specified by a diploid gene located in the terminal repeats of the l component of herpes simplex virus genome. | in the course of studies on the a sequences located at the termini of and at the junction between the l and s components of herpes simplex virus 1 dna, j. chou and b. roizman (j. virol. 57:629-637, 1986) noted that the a sequence acted as a gamma 1 promoter when fused to the structural sequence of the thymidine kinase gene, the b inverted repeat sequences located in the l component next to the a sequences contained an open reading frame predicted to encode the protein of 358 amino acids with a m ... | 1986 | 3009891 |
| radioreceptor assay of anti-tsh receptor antibody activity: comparison of assays using unextracted serum and immunoglobulin fractions, and standardization of expression of activities. | a radioreceptor assay for tsh receptor antibodies developed by shewring and smith is described in which unextracted serum is used. the assay is simple and reproducible. tsh-binding inhibitor immunoglobulin (tbii) activity determined using unextracted serum correlated well with that determined using the immunoglobulin fraction purified with polyethylene glycol. the assay detected tsh receptor antibodies in 81 of 85 patients (95%) with untreated graves' disease, no patients with graves' disease in ... | 1986 | 3016274 |
| anti-htlv-iii screening of blood donors. | in a survey on 82,383 blood donor specimens from hessen, west germany, the observed percentage of repeatedly positive htlv-iii/lav antibody elisa results was 0.2%, but only 0.018% were confirmed by western blot, immunofluorescence assay or radioimmunoprecipitation assay. these data are consistent with larger epidemiological data compiled from other german blood banks and suggest a high degree of nonspecific false-positive results; one of the causes for false-positives are hla-dr4 antibodies, whi ... | 1986 | 3017001 |
| effects of theophylline and camp on amphiuma small intestine. | | 1986 | 3017122 |
| effect of age on the formation of cyclic nucleotides in guinea-pig tracheal smooth muscle in response to pharmacological agents. | the effects of isoproterenol, carbachol and other drugs on the cyclic amp and cyclic gmp levels in tracheal smooth muscles of guinea-pigs of four different ages were investigated. isoproterenol increased the cyclic amp level several-fold in tracheal muscles from newborn (1 week) and young (4-7 weeks) guinea-pigs, but it caused less increase in the level in muscles from middle-aged (12 weeks) and old (20-24 weeks) guinea-pigs, although the basal cyclic amp level at these ages was not significantl ... | 1986 | 3017724 |
| steroid-responsive bronchiolitis after human heart-lung transplantation. | this report describes the clinical course of a patient who developed obliterative bronchiolitis after viral infection on three separate occasions. long-term follow-up is given. it is suggested that the syndrome of late pulmonary deterioration after transplantation may be steroid responsive if treatment is initiated early in the natural history of the syndrome. in addition, it is suggested that increased emphasis should be placed on the documentation of viral infection in transplant recipients to ... | 1986 | 3018383 |
| testing for htlv-iii/lav antibody. | | 1986 | 3019171 |
| studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man. | the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ... | 1986 | 3019702 |
| studies on the antihypertensive effect of single doses of the angiotensin converting enzyme inhibitor ramipril (hoe 498) in man. | the time course of the blood pressure lowering effect and the dose-response relationship of the new angiotensin converting enzyme inhibitor ramipril (hoe 498) were studied in 8 patients, with essential hypertension. as compared with placebo, a single oral dose of 2.5 mg ramipril lowered systolic and diastolic blood pressure. the antihypertensive action of single oral doses of 5, 7.5 and 10 mg ramipril was more pronounced. no change in heart rate occurred. angiotensin converting enzyme activity w ... | 1986 | 3019702 |
| animal models for retrovirus-induced immunodeficiency disease. | | 1986 | 3019879 |
| animal models for retrovirus-induced immunodeficiency disease. | | 1986 | 3019879 |
| isolation, characterization, and mapping of tn5 insertions into the 140-megadalton invasion plasmid defective in the mouse sereny test in shigella flexneri 2a. | using shigella flexneri 2a ysh6000, we isolated 304 independent tn5 insertion mutants in the 230-kilobase invasion plasmid, pmysh6000. the site of each tn5 insertion was assigned to 23 sali fragments on the previously made sali cleavage map of pmysh6000. among the 304 insertions, 150 were negative in expression of four phenotypes examined (mouse sereny test [ser], invasion into epithelial cells [inv], congo red binding [pcr], and inhibition of bacterial growth [igr] ): 12 were ser- inv+ pcr+ igr ... | 1986 | 3019891 |
| immortalization of human t lymphocytes by htlv-i: phenotypic characteristics of target cells and kinetics of virus integration and expression. | in-vitro infection of normal human lymphocytes with htlv-i (human t-cell lymphotropic retrovirus type i) has been carried out to study the target cell specificity and the kinetics of infection. cord blood (cbl) and adult peripheral blood lymphocytes (pbl) have been co-cultivated with irradiated htlv-i donor cells (mt2 and c91pl lines). established ('immortalized') htlv-i positive cell lines were obtained only from cbl: in comparison with pbl, a less mature phenotype of t-cell subsets and a lower ... | 1986 | 3020320 |
| substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns. | the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ... | 1986 | 3020427 |
| substrate phosphoprotein availability regulates eclosion hormone sensitivity in an insect cns. | the final step in the moulting of all insects is ecdysis, the shedding of the cuticle of the previous instar, which is triggered in lepidoptera by the neurosecretory peptide eclosion hormone. this hormone acts directly on the nervous system to release the appropriate motor patterns for larval, pupal and adult ecdysis, but there are only brief periods near the end of each moult when the nervous system is competent to respond to the hormone. previous experiments have shown that the action of eclos ... | 1986 | 3020427 |
| differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro. | transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ... | 1986 | 3020557 |
| differential effects of transforming growth factor type beta on the growth and function of adrenocortical cells in vitro. | transforming growth factor type beta (tgf-beta) suppresses basal as well as corticotropin (acth)-stimulated steroid formation by bovine adrenocortical cells in culture. the effect is dose dependent and is not accompanied by any change in adrenocortical cell growth. the minimum effective dose of tgf-beta is 4 x 10(-13) m (10 pg/ml), and maximal inhibition is observed at a concentration of 4 x 10(-11) m (1 ng/ml). a 16- to 20-hr incubation with tgf-beta is required to decrease steroidogenesis, and ... | 1986 | 3020557 |
| exposure of sheep to natural aerosols of foot-and-mouth disease virus. | sheep taken individually and allowed to inhale air being drawn along a duct from a cabinet containing pigs acutely infected with foot-and-mouth disease virus for 10 or 15 minute periods were infected by doses as low as 10 tcid50 of virus. the most consistent and reliable indicators of infection were viraemia and seroconversion. the mean times from exposure to onset of viraemia, pyrexia and the appearance of vesicular lesions were 2.5, 3.8 and 4.7 days, respectively. neither the time from exposur ... | 1986 | 3020658 |
| diminishing morbidity and mortality of bone marrow transplantation. | the reasons for the operational failure of bone marrow transplantation (bmt) are familiar and varied. they are the following: nonmarrow toxicity of the preparative regimen, marrow toxicity of the preparative regimen, acute graft-versus-host disease (gvhd), opportunistic infections, especially viral infections and recurrence of hematologic malignancy in the case of transplants for leukemia. this review examines operational failure of bmt and presents measures for its prevention. | 1986 | 3020798 |
| dna sequence and product analysis of the virf locus responsible for congo red binding and cell invasion in shigella flexneri 2a. | the dna sequence of virf, a locus associated with virulence and the ability to bind congo red in shigella flexneri 2a that is located on a 140-megadalton (230-kilobase) plasmid, was determined and analyzed. it was rich in a and t. the direction of transcription of virf was determined by using a chloramphenicol resistance cartridge. an open reading frame readable in this direction was found. three proteins, 30, 27, and 21 kilodaltons, all corresponding to those predicted from the above sequence, ... | 1986 | 3021627 |
| characterization of shigella flexneri sequences encoding congo red binding (crb): conservation of multiple crb sequences and role of is1 in loss of the crb+ phenotype. | the ability to bind congo red (crb+) is associated with virulence of shigella flexneri and is encoded by a large, 220-kilobase plasmid. we cloned fragments of this plasmid to isolate the sequences encoding congo red binding, to determine the degree of conservation of these sequences among s. flexneri strains, and to study the molecular basis for loss of the crb+ phenotype. at least two separate bamhi fragments cloned into plasmid vectors encode congo red binding in e. coli or s. flexneri. one cr ... | 1986 | 3021630 |
| human leukocyte antigens and sister chromatid exchanges in families with multiple adenomatosis coli. | to study human leukocyte antigens (hla) and sister chromatid exchanges (sce) in multiple adenomatosis coli (mac), eight families were evaluated. twenty-five subjects (14 affected members and 11 healthy relatives) were studied with hla a, b, and c typing of peripheral lymphocytes and sce count in cells that had replicated twice. the results of the present immunogenetic study, although limited to a small number of cases, suggest a correlation between hla, sce, and mac. when the results of further ... | 1986 | 3022927 |
| partial transformation of mouse fibroblastic and epithelial cell lines with the v-myc oncogene. | to investigate the role of the myc gene in mammalian cell transformation, plasmid constructs containing the v-myc oncogene and a co-selectable g418 resistance marker were introduced into both mouse fibroblasts (nih-3t3) and bladder epithelial cells (bbn3 and bbn7). after transfection or microinjection of dna, no transformed foci could be detected on confluent monolayers but, when the cells were cultured under conditions in which individual cells were allowed to grow and form colonies, morphologi ... | 1987 | 3023119 |
| total iridectomy does not alter outflow facility responses to cyclic amp in cynomolgus monkeys. | total outflow facility was measured by two-level constant pressure anterior chamber perfusion in surgically untouched and totally iridectomized cynomolgus monkey eyes receiving bolus intracameral infusions of cyclic amp, dibutyryl cyclic amp, or 5'amp. cyclic amp doses of 50 and 100 micrograms (corresponding to initial intracameral concentrations of 1.5- and 3 mm) increased facility by approximately 20 and 40% respectively in both surgically untouched and aniridic eyes. higher doses produced no ... | 1986 | 3023122 |
| a conserved human germline v kappa gene directly encodes rheumatoid factor light chains. | the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people. | 1986 | 3023521 |
| a conserved human germline v kappa gene directly encodes rheumatoid factor light chains. | the full-length gene that encodes the light chain variable regions of an idiotypically related group of human igm kappa rheumatoid factors (rfs) has been cloned and sequenced. the deduced amino acid sequence is identical to four separate rf proteins. these results prove that genes capable of encoding human anti-igg autoantibody light chains without any somatic mutation are present in the kappa gene repertoire of normal people. | 1986 | 3023521 |
| structure of replicating simian virus 40 minichromosomes. the replication fork, core histone segregation and terminal structures. | the structure of replicating simian virus 40 (sv40) minichromosomes was studied by dna crosslinking with trimethyl-psoralen. the procedure was used both in vitro with extracted sv40 minichromosomes as well as in vivo with sv40-infected cells. both procedures gave essentially the same results. mature sv40 minichromosomes are estimated to contain about 27 nucleosomes (error +/- 2), except for those molecules with a nucleosome-free gap, which are interpreted to contain 25 nucleosomes (error +/- 2). ... | 1986 | 3023620 |
| relative activities of 5'-nucleotidase and adenosine deaminase in atrial and ventricular myocardium--the enzyme paradox. | the 5'-nucleotidase and adenosine deaminase activities were determined for tissue extracts of up to 10 defined regions of the normal dog heart. the specific activity of 5'-nucleotidase was very significantly higher in atrial than in ventricular myocardium (4 x) and was also higher in right than in left sites of the heart (2 x). the reason for the markedly higher specific activity in atrial muscle is not clear and appears paradoxical relative to adenine nucleotide content and pattern of atp decay ... | 1986 | 3025011 |
| structurally related bacillus thuringiensis delta-endotoxins display major differences in insecticidal activity in vivo and in vitro. | many strains within the 22 serotypes of bacillus thuringiensis produce crystal delta-endotoxins with slight differences in their insecticidal toxicity spectrum in vivo. since the basis of this specificity is unknown, we chose to compare the activity of delta-endotoxins from three strains: b. thuringiensis var. kurstaki hd-1, var. aizawai hd-249 and var. thuringiensis hd-350, both in vivo and on insect cell lines in vitro. immunoblotting with antisera to activated var. kurstaki p1 lepidopteran to ... | 1986 | 3027111 |
| nucleotide sequence of the virulence gene virg of the agrobacterium tumefaciens octopine ti plasmid: significant homology between virg and the regulatory genes ompr, phob and dye of e. coli. | the entire nucleotide sequence of the virg locus, from the octopine ti plasmid of agrobacterium tumefaciens strain 15955, has been determined. the virg gene is 801 nucleotides in length and has one open reading frame which encodes a protein of mr 29,995. the virg gene is involved in the transcriptional activation of the ti plasmid vir-loci, which occurs after induction by specific compounds present in plant exudate. sequence analysis of the agrobacterium virg protein showed significant homology ... | 1986 | 3027669 |
| the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences. | repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ... | 1986 | 3028726 |
| the effects of electroconvulsive therapy and antidepressant drugs on monoamine receptors in rodent brain--similarities and differences. | repeated administration to rodents of electroconvulsive shock (ecs) produces changes in brain monoamine biochemistry and function, several of which are also seen after repeated administration of antidepressant drugs. both repeated ecs and antidepressant drug administration decrease cortical beta-adrenoceptor density and attenuate the alpha 2-adrenoceptor-mediated sedation response to clonidine injection. neither procedure alters phenylephrine-induced locomotor activity in mice, a measure of alph ... | 1986 | 3028726 |
| reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. | we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ... | 1986 | 3028771 |
| reconstitution of vesicle fusions occurring in endocytosis with a cell-free system. | we have used defined subcellular fractions to reconstitute in a cell-free system vesicle fusions occurring in the endocytic pathway. the endosomal fractions were prepared by immuno-isolation using as antigen an epitope located on a foreign protein, the transmembrane glycoprotein g (g-protein) of vesicular stomatitis virus. the g-protein was first implanted in the cell plasma membrane and subsequently endocytosed for 15 to 30 min at 37 degrees c. the endosomal fractions were immuno-isolated on a ... | 1986 | 3028771 |
| phospholipase c contracts visceral smooth muscle. | phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ... | 1986 | 3028825 |
| phospholipase c contracts visceral smooth muscle. | phospholipase c added into the muscle bath (0.2-1.5 units/ml) contracted the guinea-pig taenia coli in a concentration-dependent manner. the fully developed contraction appeared within second, like the contractile effect evoked by 5 microm acetylcholine. pharmacochemical dissection (using phospholipase d, indomethacin, neomycin, li+, phorbol ester and oleoyl-acetyl-glycerol) showed that the contractions induced by exogenous phospholipase c are mediated by inositol trisphosphate, not by diacylgly ... | 1986 | 3028825 |
| virulence of iron transport mutants of shigella flexneri and utilization of host iron compounds. | mutants of shigella flexneri defective in aerobactin-mediated iron transport were assayed for virulence in several model systems. a tn5 insertion mutant was invasive in hela cells, lethal in the chicken embryo, and produced keratoconjunctivitis in the guinea pig, indicating little or no loss of ability to invade and multiply intracellularly. although the mutant failed to grow in low-iron medium in vitro, growth equivalent to that of the wild type was observed in hela cell lysates. thus, there ap ... | 1987 | 3028962 |
| porous hydroxyapatite as a bone graft substitute in diaphyseal defects: a histometric study. | porous hydroxyapatite (ip200), formed by conversion of the poritidae porites exoskeleton, has pores averaging 230 microns and pore interconnections averaging 190 microns in diameter. in the distal radial diaphyses of 14 dogs, bilateral 7.5 x 20 mm cortical windows were created and fitted with 5 x 7.5 x 20 mm blocks of ip200 implants and iliac autografts. both implanted and contralateral grafted radius specimens were retrieved at 3, 6, 12, 24, and 48 months. unstained undecalcified sections were ... | 1987 | 3029358 |
| on the fidelity of dna replication: herpes dna polymerase and its associated exonuclease. | procaryotic dna polymerases contain an associated 3'----5' exonuclease activity which provides a proofreading function and contributes substantially to replication fidelity. dna polymerases of the eucaryotic herpes-type viruses contain similar associated exonuclease activities. we have investigated the fidelity of polymerases purified from wild type herpes simplex virus, as well as from mutator and antimutator strains. on synthetic templates, the herpes enzymes show greater relative exonuclease ... | 1987 | 3029700 |
| a cellular protein binds to a conserved sequence in the adenovirus type 2 enhancer. | a sensitive gel retention assay has been utilized to detect proteins from uninfected hela nuclei which interact with the adenovirus type 2 enhancer. this assay has been employed to monitor fractionation of nuclear extracts. three enhancer binding factors were resolved by chromatography on deae-sepharose and one of the factors was further purified by chromatography on heparin-sepharose. dnase protection experiments have shown that the heparin-sepharose fraction contains a factor which binds predo ... | 1987 | 3031610 |
| integration and expression of provirus in human cells transformed by avian sarcoma virus. | previously, human diploid fibroblasts from some donors infected in vitro by avian sarcoma virus (asv) were transformed and found, by electron microscopy, to produce small numbers of virus particles that were infectious by bioassay; also, a line of human osteosarcoma cells infected with asv developed additional characteristics of transformation and released a small number of infectious virus particles. in this study the complete proviral sequence was shown to be integrated in the genome of these ... | 1987 | 3033382 |
| effects of inhibitors of the cytoplasmic structures and functions on the early phase of infection of cultured cells with simian virus 40. | to obtain information about cytoplasmic structures and functions involving the entry of simian virus 40 virions into cells, we examined whether the inhibitors that affect the functions and/or structure of lysosomes, cell membrane, and cytoskeletons inhibit expression of nuclear t antigen in the sv40-inoculated rat 3y1 and monkey cv-1 cells. chloroquine, methylamine, and butylamine did not inhibit t-antigen expression, suggesting that lysosomal acidification is not required for establishment of i ... | 1987 | 3033894 |
| the effect of cycloleucine on sfv a7(74) infection in mice. | cycloleucine (cl), a non-metabolizable amino acid analogue, was found to reduce thymus and spleen weights in semliki forest virus (sfv) strain a7(74) infected and control mice. the maximum effects were seen when three daily doses of cl were given to mice 24 h after an sfv a7(74) infection. in these mice thymus atrophy led to abolition of thymus dependent immune responses and changes in the pathological features of the viral infection--the most striking feature being prevention of demyelination. ... | 1987 | 3034321 |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| chronic food restriction and the circadian rhythms of pituitary-adrenal hormones, growth hormone and thyroid-stimulating hormone. | adult male sprague-dawley rats were subjected to food restriction so that they ate 65% of food ingested by control rats. while control rats had free access to food over the 24-hour period, food-restricted rats were provided with food daily at 10 a.m. the experimental period lasted for 34 days. on day 35, rats from both experimental groups were killed at 08.00, 11.00, 14.00, 24.00 and 02.00 h. food restriction modified the circadian rhythms of acth and corticosterone. in addition, total circulati ... | 1987 | 3035995 |
| [expressing plasmid vectors on the basis of escherichia coli beta-galactosidase gene fragments]. | with the use of synthetic dna fragments, a set of new plasmid vectors has been obtained. the vectors provided high level expression of peptides and small proteins in e. coli as fusions with fragments of beta-galactosidase of various length. these vectors were used to achieve expression of a synthetic gene for a functionally active fragment of bacteriorhodopsin. the yields of hybrid proteins consisting of beta-galactosidase and bacteriorhodopsin fragments were in the range of 5-30% from the total ... | 1987 | 3036168 |
| a high-yield, high-purity elutriation method for preparing human granulocytes demonstrating enhanced experimental lifetimes. | a counterflow centrifugal elutriation procedure for preparing human granulocytes is described. this method permits isolation of 1-2 x 10(9) granulocytes with a yield of 70% +/- 7% from a unit of whole blood in 3 h. these cells are 99% +/- 1% pure and 95-99% viable. when compared to cells prepared by a conventional procedure employing hypotonic lysis to remove red cells, the elutriated cells show enhanced oxidative responses resulting from enhanced longevity. other responses including receptor-me ... | 1987 | 3036986 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| structure of the 3' region of the human elastin gene: great abundance of alu repetitive sequences and few coding sequences. | two overlapping clones encompassing 8.5 kb of the human elastin gene were isolated from two genomic libraries constructed by partial digestion with either haeiii/alui or sau3a and contained in lambda charon 4a or embl3, respectively. the 6 kb of dna comprising the most 3' portion of the gene were sequenced demonstrating an extremely low coding ratio since only three exons containing a total of 134 translated nucleotides were identified. two exons totaling 78 bp of translated sequences which were ... | 1987 | 3038460 |
| complete amino acid sequence of cytochrome c551 from erythrobacter species strain och 114. | the complete amino acid sequence of cytochrome c551 isolated from an aerobic photosynthetic bacterium, erythrobacter sp. strain och 114, was determined. the cytochrome molecule was composed of a total of 119 amino acid residues and its molecular weight including heme was calculated to be 13,235. the sequence was (sequence: see text). its molecular weight indicates that this cytochrome is of the l-type. sequence alignment with other bacterial cytochromes c shows that this cytochrome is similar to ... | 1987 | 3038859 |
| [functional macrophage activity in infection with virulent and avirulent strains of the dysentery microbe]. | the effect of s. flexneri virulent and avirulent (vaccine) strains 2a on the cytoplasmic membrane of mouse macrophages has been studied by evaluating the action of these bacteria on the activity of 5-nucleotidase. the dynamics of the activity of 5-nucleotidase after the introduction of both virulent and avirulent strains has a phasic character with alternating rises and falls in the activity of this enzyme in comparison with the control. s. flexneri vaccine strain produces mainly a stimulating e ... | 1987 | 3039764 |