| expression of helicobacter pylori urease genes in escherichia coli grown under nitrogen-limiting conditions. | helicobacter pylori produces a potent urease that is believed to play a role in the pathogenesis of gastroduodenal diseases. four genes (urea, ureb, urec, and ured) were previously shown to be able to achieve a urease-positive phenotype when introduced into campylobacter jejuni, whereas escherichia coli cells harboring these genes did not express urease activity (a. labigne, v. cussac, and p. courcoux, j. bacteriol. 173:1920-1931, 1991). results that demonstrate that h. pylori urease genes could ... | 1992 | 1313413 |
| cloning and characterization of a gene required for the secretion of extracellular enzymes across the outer membrane by xanthomonas campestris pv. campestris. | nonpathogenic mutants of xanthomonas campestris pv. campestris, generated from transposon mutagenesis, accumulated extracellular polygalacturonate lyase, alpha-amylase, and endoglucanase in the periplasm. the transposon tn5 was introduced by a mobilizable, suicidal plasmid, psup2021 or peydg1. genomic banks of wild-type x. campestris pv. campestris, constructed on the broad-host-range, mobilizable cosmid plafr1 or plafr3, were conjugated with one of the mutants, designated xc1708. recombinant pl ... | 1992 | 1313415 |
| nucleotide sequence and structure of the klebsiella pneumoniae pqq operon. | a 6940 bp klebsiella pneumoniae chromosomal dna fragment, containing genes involved in pyrroloquinoline quinone (pqq) biosynthesis, was sequenced. six open reading frames, pqqa, pqqb, pqqc, pqqd, pqqe and pqqf were identified in the pqq operon, which coded for polypeptides of 2764 (23 amino acids), 33,464, 28,986, 10,436, 42,881 and 83,616 da, respectively. the transcription startpoint was mapped by primer extension analysis, upstream of pqqa, and promoter boxes could be identified. the gene pro ... | 1992 | 1313537 |
| venereal disease. | equine venereal infections of concern in the united states include ehv-3, t. equigenitalis, p. aeruginosa, and k. pneumoniae. stallions may also harbor eav in the genital tract and transmit the virus to mares during coitus. with the exception of ehv-3, the stallion generally remains asymptomatic while transmitting infections to mares during breeding. methods for diagnosis, treatment, and control of these infections are discussed. | 1992 | 1315616 |
| a comparison of the safety and efficacy of lomefloxacin and ciprofloxacin in the treatment of complicated or recurrent urinary tract infections. | the efficacy and safety of two fluoroquinolone antimicrobial agents, lomefloxacin and ciprofloxacin, were compared in a randomized, investigator-blinded, multiple-dose study that enrolled 150 adult outpatients with complicated or recurrent urinary tract infections. a total of 75 patients were treated with 400 mg of lomefloxacin once daily for 10-14 days; an equal number of patients received 500 mg of ciprofloxacin every 12 hours for 10-14 days. both groups of patients were comparable in terms of ... | 1992 | 1316077 |
| a double-blind study of two dosage regimens of lomefloxacin in bacteriologically proven exacerbations of chronic bronchitis of gram-negative etiology. | lomefloxacin has been shown to produce high and sustained concentrations in serum and bronchial mucosa after once-daily administration. this study was designed to assess whether a dose response exists for 400 mg lomefloxacin given once daily or twice daily for 10 days in the treatment of acute bacterial exacerbations of chronic bronchitis of gram-negative etiology. a total of 100 adult patients with acute exacerbations of chronic bronchitis were enrolled at 10 study sites in germany. patients wi ... | 1992 | 1316079 |
| antibacterial activity of the new quinolone ci-960 (pd 127391) against clinical isolates at a major tertiary care center in saudi arabia. | the antibacterial activity of the new fluoroquinolone ci-960 (pd 127391) was evaluated against 1,162 clinical isolates and compared with other quinolones and various commonly used antibiotics. ci-960 was highly effective against members of enterobacteriaceae inhibiting 626 of the 629 isolates at a less than or equal to 0.03 to 0.12 microgram/ml concentration. all the 305 isolates of pseudomonas aeruginosa, xanthomona maltophilia, and acinetobacter were susceptible to ci-960. it was the only effe ... | 1992 | 1317281 |
| in vitro and in vivo antibacterial activities of a new quinolone, opc-17116. | the in vitro and in vivo antibacterial activities of opc-17116 were compared with those of ofloxacin, enoxacin, ciprofloxacin, and tosufloxacin. the mics of opc-17116 for 90% of the strains tested were 0.125 to 8 micrograms/ml against gram-positive bacteria such as members of the genera staphylococcus, streptococcus, and enterococcus: less than or equal to 0.063 to 16 micrograms/ml against members of the family enterobacteriaceae; and less than or equal to 0.063 to 16 micrograms/ml against gluco ... | 1992 | 1320364 |
| morphologic features and hydrophobicity of the cell surface of mycoplasma hyopneumoniae. | cell surface hydrophobicity of mycoplasma hyopneumoniae was evaluated by phase partitioning in a hydrocarbon-aqueous mixture, by hydrophobic interaction chromatography, and by salting out with ammonium sulfate. results obtained by use of these techniques gave evidence that the cell surface of m hyopneumoniae is weakly hydrophobic, compared with strongly hydrophobic staphylococcus aureus cowan i and hydrophilic klebsiella pneumoniae. after treatment of the organisms with trypsin, m hyopneumoniae ... | 1992 | 1323225 |
| the in-vitro activity of two new quinolones: rufloxacin and mf 961. | the in-vitro activity of two new quinolone antimicrobials, rufloxacin and mf 961, together with the desmethylated metabolite of rufloxacin (mf 922) were compared with other orally administered agents against 622 bacterial strains. against enterobacteriaceae and pseudomonas aeruginosa rufloxacin was generally active (mic90 1-8 mg/l) with the exception of klebsiella and serratia spp. (mic90 32 mg/l and enterobacter spp. (mic90) 64 mg/l. the respiratory pathogens haemophilus influenzae and moraxell ... | 1992 | 1324239 |
| cloning and expression of the genes for xylose isomerase and xylulokinase from klebsiella pneumoniae 1033 in escherichia coli k12. | the genes xyla and xylb were cloned together with their promoter region from the chromosome of klebsiella pneumoniae var. aerogenes 1033 and the dna sequence (3225 bp) was determined. the gene xyla encodes the enzyme xylose isomerase (xi or xyla) consisting of 440 amino acids (calculated m(r) of 49,793). the gene xylb encodes the enzyme xylulokinase (xk or xylb) with a calculated m(r) of 51,783 (483 amino acids). the two genes successfully complemented xyl mutants of escherichia coli k12, but no ... | 1992 | 1324398 |
| [microbial ecology of the digestive tract in patients with acute intestinal infections]. | | 1992 | 1324548 |
| effects of tripeptides derived from milk proteins on polymorphonuclear oxidative and phosphoinositide metabolisms. | the tripeptide glf (glycyl-leucyl-phenylalanine) was isolated from human milk proteins. this peptide increased phagocytosis by human and murine macrophages and protected mice against klebsiella pneumoniae infection. specific binding sites on human polymorphonuclear leukocytes (pmns) have been demonstrated recently. the aim of the present research was to study the action of this peptide on rat and human pmn oxidative burst and to investigate the consequences of cell stimulation on polyphosphoinos ... | 1992 | 1324681 |
| scintigraphy with j001 macrophage targeting glycolipopeptide. a new approach for sarcoidosis imaging. | scintigraphy with radiolabeled j001 as a ligand for macrophage targeting is a new approach for sarcoidosis imaging. j001 is a fully characterized acylated peptido-poly (1,3) galactoside isolated from klebsiella membrane proteoglycans and able to bind electively recruited macrophages. its physiochemical properties allow rapid absorption by the respiratory tract when this agent, labeled by 99m technetium, is administered as an aerosol. images are obtained within 3 to 5 h after inhalation. in the p ... | 1992 | 1325338 |
| in vitro evaluation of e-4695, a new fluoro-naphthyridine. | compound e-4695, a c-7 azetidinyl fluoro-naphthyridine, was compared to six structurally related quinolones and was generally two- to four-fold more active than ciprofloxacin. e-4695 was particularly active against staphylococcus aureus (mic90 0.06 mg/l), staphylococcus haemolyticus (mic90 0.5 mg/l), klebsiella spp. (mic90 less than or equal to 0.015 mg/l), serratia marcescens (mic90 0.06 mg/l), acinetobacter spp. (mic90 less than or equal to 0.015 mg/l), pseudomonas aeruginosa (mic90 0.5 mg/l), ... | 1992 | 1327787 |
| comparative in vitro activity of sparfloxacin (at 4140, rp 64206--spfx) against 275 multiresistant clinical isolates. | the in-vitro activity of sparfloxacin was compared with that of pefloxacin, ofloxacin, ciprofloxacin, imipenem, ceftazidime, gentamicin and amikacin against 275 multiresistant nosocomial clinical isolates. they consisted of pseudomonas aeruginosa (37), enterobacter cloacae (42), acinetobacter anitratus (60), klebsiella pneumoniae (37) and staphylococcus sp (99). minimum inhibitory concentrations (mic90) and geometric mean mics for sparfloxacin were as follows (mg/l): p. aeruginosa 128-23.7, e. c ... | 1992 | 1328554 |
| piperacillin, tazobactam, and gentamicin alone or combined in an endocarditis model of infection by a tem-3-producing strain of klebsiella pneumoniae or its susceptible variant. | the efficacy of tazobactam, a beta-lactamase inhibitor, in combination with piperacillin, was studied in vitro and in rabbit experimental endocarditis due to a klebsiella pneumoniae strain (kpr) producing an extended-spectrum beta-lactamase, tem-3, or its nonproducing variant (kps). in vitro, piperacillin was active against kps (mic = 4 micrograms/ml, mbc = 8 micrograms/ml with 10(7)-cfu/ml inoculum) but not against kpr (mic = mbc = 256 micrograms/ml). tazobactam (1 microgram/ml) restored the ac ... | 1992 | 1329634 |
| transposition of the gene encoding a tem-12 extended-spectrum beta-lactamase. | an isolate of klebsiella oxytoca from the blood culture of a child with leukemia was found to produce two beta-lactamases, at least one of which conferred resistance to ceftazidime. genes encoding both enzymes were located on a single self-transmissible 100-kb plasmid, poz201. this plasmid was introduced into escherichia coli ub5201 (pacyc184), and the gene encoding one beta-lactamase was transposed onto plasmid pacyc184 by exploiting a gene dosage effect. the transposable gene was found to enco ... | 1992 | 1329636 |
| sequence of the sodium ion pump oxaloacetate decarboxylase from salmonella typhimurium. | a genomic library of salmonella typhimurium dna was constructed in the lambda-phage embl3 and screened by immunoblotting for expression of the oxaloacetate decarboxylase alpha-subunit. after subcloning on plasmids the entire sequence of the oxaloacetate decarboxylase was determined. the genes encoding subunits gamma (oadg), alpha (oada), and beta (oadb) of the decarboxylase are clustered on the chromosome in that order. a typical consensus sequence of a promoter is not found upstream of the oadg ... | 1992 | 1331067 |
| plasma bactericidal activity of a new c-5 methyl fluoroquinolone after oral doses of 400 and 800 mg. | the plasma bactericidal activity of a new c-5 methyl fluoroquinolone, opc-17116, was determined after once-daily oral ingestion of 400 mg and 800 mg in normal, healthy volunteers. opc-17116 at a 400-mg dose produced plasma bactericidal titers greater than or equal to 1:16 at 12 hours against escherichia coli, klebsiella pneumoniae, serratia marcescens, haemophilus influenzae, and moraxella catarrhalis. opc-17116 bactericidal titers against pseudomonas aeruginosa were 1:2 or 1:4 at 6 and 12 hours ... | 1992 | 1331205 |
| in vitro and in vivo antibacterial activities of am-1155, a new 6-fluoro-8-methoxy quinolone. | am-1155 is a new quinolone with a wide spectrum of antibacterial activity against various bacteria including anaerobes and mycoplasma pneumoniae. am-1155 was 2- to 16-fold more active than ciprofloxacin and ofloxacin against staphylococcus aureus including methicillin-resistant strains, staphylococcus epidermidis, streptococcus pneumoniae, and enterococcus faecalis; its mics for 90% of strains tested were 0.10 to 0.78 micrograms/ml. the activity of am-1155 was comparable to that of ciprofloxacin ... | 1992 | 1332587 |
| comparative in vitro activities of a new quinolone, opc-17116, possessing potent activity against gram-positive bacteria. | the in vitro antibacterial activity of opc-17116, a new fluoroquinolone, against a wide variety of clinical isolates was evaluated and compared with those of ciprofloxacin, ofloxacin, and norfloxacin. opc-17116 showed potent broad-spectrum activity against gram-positive and -negative bacteria. the activity of this compound against gram-positive bacteria was higher than those of other quinolones, and its activity against gram-negative and anaerobic bacteria was roughly comparable to those of othe ... | 1992 | 1332589 |
| rapid plasmid dna isolation from mucoid gram-negative bacteria. | exopolysaccharides interfere with the isolation and characterization of plasmid dna from gram-negative bacteria. to repress capsular polysaccharide production, bacteria were cultured in medium containing bismuth nitrate and sodium salicylate. rapid removal of other contaminating bacterial surface components was achieved by mild acidic zwitterionic detergent extraction. after treatment, bacterial cells were more readily lysed in alkaline detergents. the resulting plasmid preparations contained vi ... | 1992 | 1333482 |
| resistance to amoxicillin/clavulanate in escherichia coli. | | 1992 | 1334325 |
| the distribution of the dhfr genes in trimethoprim-resistant urinary tract isolates from taiwan. | between july 1987 and june 1989, 1054 urinary isolates of enterobacteria from kaohsiung, taiwan were studied for their trimethoprim resistance. trimethoprim resistance was defined as mic greater than 4 micrograms/ml and high-level resistance by mic greater than 1000 micrograms/ml. the incidence of trimethoprim resistance increased from 33.6% in 1987 to 42.1% in 1989. among the resistant strains studied, 90% were resistant to high levels of trimethoprim. an increase in the proportion of resistant ... | 1992 | 1334841 |
| [annual changes of bacteria isolated from urinary tract infections and their susceptibility to new quinolone agents]. | statistical analyses were conducted on bacterial strains isolated from the urine of patients with urinary tract infections (uti) in the department of urology at aichi medical university from january 1988 through december 1991. the drug sensitivity of the bacterial strains isolated from patients with complicated uti between 1988 and 1991 was analyzed and compared. among the outpatients with uncomplicated uti, a total of 352 strains were isolated. e. coli was isolated the most frequently (90.1%). ... | 1992 | 1336639 |
| characterization of the citrobacter freundii phoe gene and development of c. freundii-specific oligonucleotides. | the phoe gene of citrobacter freundii, encoding a pore-forming outer membrane protein, was cloned and its nucleotide sequence was determined. the homologies in terms of identical amino acids between the c. freundii phoe protein and those of escherichia coli, e. cloacae and klebsiella pneumoniae were 90%, 86% and 84%, respectively. two synthetic oligonucleotides, corresponding to hypervariable, cell surface-exposed regions of the protein, were tested for their specificity in polymerase chain reac ... | 1992 | 1337052 |
| streptomyces atp nucleotide 3'-pyrophosphokinase and its gene. | streptomyces atp nucleotide 3'-pyrophosphokinase is an extracellular, ribosome-independent, and stringent factor-mimic ppgpp synthetase with an unusually broad acceptor spectrum. the gene-containing dna fragments cloned from chromosomal dna of a producer s. morookaensis into pij699 and puc plasmids were found to express the active enzyme in the transformed s. lividans tk24 and enteric e. coli jm109 and nitrogen-fixing klebsiella pneumoniae m5a1 and 5022, respectively. base sequence of the struct ... | 1992 | 1337783 |
| susceptibility of recent clinical isolates to temafloxacin (a-63004) and other antimicrobial agents. | in vitro susceptibility of 1008 strains of recent clinical isolates was determined against the new aryl fluoroquinolone temafloxacin (t-167, a-63004) ciprofloxacin, norfloxacin, ampicillin, piperacillin, cephalothin, cefoxitin, ceftazidime, gentamicin, amikacin, oxacillin and vancomycin. the minimum inhibitory concentrations (mics) of temafloxacin in micrograms/ml required for > or = 90% isolates were 0.13-0.5 for enterobacter, 0.03-0.25 for escherichia coli, 0.12-0.5 for klebsiella, 0.5-1.0 for ... | 1992 | 1338041 |
| interaction of klebsiella capsule type 7 with human polymorphonuclear leucocytes. | klebsiella serotype k7 is found among the capsule types that are most prevalent in respiratory tract isolates. to evaluate the significance of the k7 antigen in bacteria-leucocyte interactions, k7-encapsulated klebsiella pneumoniae strains and their non-capsulate mutants were investigated. the k7 isolates were compared to k2-capsulate strains and their respective k- derivatives. k7-capsulate bacteria were less hydrophilic, and more readily phagocytosed and killed by human polymorphonuclear leuco ... | 1992 | 1338474 |
| opsonic capacity of a hyperimmune serum against outer membrane proteins of klebsiella pneumoniae. | the opsonic capacity of a hyperimmune rabbit serum against a porin-rich outer membrane protein preparation of a strain of k. pneumoniae was evaluated. by immunoblot, the antiserum recognized mainly the porins from an outer membrane protein preparation. using an elisa, the titer of anti-porin antibodies was determined. through a chemiluminescence assay, the increase in the respiratory burst of murine hyperimmune serum was recorded. these data correlate with the results of the microbicidal assays ... | 1992 | 1339226 |
| primary structure and characteristics of the melibiose carrier of klebsiella pneumoniae. | the melb gene coding for the melibiose carrier of klebsiella pneumoniae was cloned and sequenced. there were two potential translation initiation sites. it was predicted that the melibiose carrier consists of 471 (or 467) amino acid residues. seventy-eight percent of the 471 amino acids were identical to the escherichia coli melibiose carrier. sugar transport characteristics were studied using an e. coli mel- mutant expressing cloned k. pneumoniae melb gene. accumulation of melibiose via the k. ... | 1992 | 1339436 |
| [urinary tract infections during the 1st month after kidney transplantation]. | the incidence and the predisposal factors of urinary tract infections (uti) in the first month post-transplant were studied in 255 kidney transplantations (252 patients). uti episodes were demonstrated in 73.7% of the grafts. the most common organisms were: escherichia coli (35.8%), staphylococcus (33.6%), streptococcus d (11.2%), klebsiella (5.3%). the infectious episodes were recurrent in 39% of the cases. the majority of the utis were asymptomatic but 7% of the infections led to septicaemia. ... | 1992 | 1340109 |
| [patterns of antimicrobial resistance in gram-negative bacilli isolated from patients in intensive care units]. | minimal inhibitory concentration test with 16 antibiotics was performed for 435 stains of gram negative bacilli, isolated from patients in intensive care units of five large hospitals. the results were analysed as to provide in vitro sensitivity patterns, as well as cross resistance between the tested drugs. the most frequent species were pseudomonas aeruginosa, e. coli, klebsiella spp., enterobacter ssp., and acinetobacter calcoaceticu, accounting for more than 80% of all isolates. best figures ... | 1992 | 1340587 |
| mobilization of nonconjugative pbr322-derivative plasmids from laboratory strains of escherichia coli to bacteria isolated from seawater. | mobilization of derivatives of plasmids pbr322 and pbr325 was shown to occur between escherichia coli k12 strains in lb-broth at 37 degrees c, provided a mobilizer plasmid (f') was present either together with the nonconjugative plasmid or in a second donor strain. evidence from restriction endonuclease analysis suggested that the mobilization was facilitated by a transposition phenomenon involving the "gamma-delta" sequence of f'. it was shown that mobilization of a derivative of pbr325 from e. ... | 1992 | 1341984 |
| in vitro antibacterial activities of ticarcillin alone and ticarcillin plus clavulanic acid against beta-lactamase producing and non-producing microorganisms. | a total of 818 clinical bacterial isolates were tested for the production of beta-lactamase by rapid chromogenic cephalosporin method and for the susceptibility to ticarcillin alone and in combination with clavulanic acid (2 micrograms/ml) by agar dilution method. these included 83 strains of methicillin-sensitive staphylococcus aureus (mssa), 31 of methicillin-resistant s. aureus (mrsa), 49 of neisseria gonorrhoeae, 58 of haemophilus influenzae, 112 of escherichia coli, 118 of klebsiella pneumo ... | 1992 | 1342000 |
| b-lymphocyte response in peripheral blood of patients with pulmonary sarcoidosis. | sarcoidosis is a granulomatous disorder which can be characterized by various immunologic abnormalities including lymphocyte dysfunctions. the purpose of this study was to investigate the b-lymphocyte reactivity in the peripheral blood of 29 various patients with pulmonary sarcoidosis. a significant decrease in the production of igg, igm and iga in supernatants of cultivated sarcoidosis b-cells both after stimulation with a t-cell dependent polyclonal b-cell activator (pokeweed mitogen) and with ... | 1992 | 1344044 |
| neonatal septicaemia due to k. pneumoniae. septicaemia due to klebsiella pneumoniae in newborn infants. nosocomial outbreak in an intensive care unit. | the authors report a nosocomial infection outbreak by klebsiella pneumoniae, observed in neonates at a gyneco-obstetrical hospital from mexico city. forty six newborns presented one or more infections due to k. pneumoniae during their stay in neonatal care units, between october 3 and november 12, 1988. sepsis was documented in 41 cases by clinical picture and routine laboratory exams, including one positive, blood culture at least. the most frequent invasive procedures practiced in these patien ... | 1992 | 1345298 |
| [preparation and use of fish fillet infusion as a basic medium for culturing bacteria]. | the authors present the first results on the utilization of fish infusion (ifp) as a basic medium for the cultivation of bacteria. the infusion was obtained from a common marine fish, corvina (micropogonias furnieri) according to the technique used in the preparation of beef infusion broth. streptococcus pyogenes, s. pneumoniae, neisseria meningitidis, campylobacter jejuni, escherichia coli, klebsiella pneumoniae, serratia marcescens, pseudomonas aeruginosa, staphylococcus aureus and bacillus su ... | 1992 | 1345299 |
| [characterization of a substance which protects klebsiella pneumoniae from the bactericidal effect of normal human serum]. | chemical composition of a substance produced by klebsiella pneumoniae which is accumulated in chemically defined medium was determined. this substance (the protective factor) protects the bacterium from being killed by normal human serum. protective factor was treated with dnase, lysozyme and an alkaline protease. the two former enzymes did not affect the protective factor. on the other hand, when alkaline protease was used, the protective activity was totally lost. results showed that the prote ... | 1992 | 1345314 |
| r-plasmid-encoded adhesive factor in klebsiella pneumoniae strains responsible for human nosocomial infections. | klebsiella pneumoniae strains involved in hospital outbreaks of nosocomial infections, such as suppurative lesions, bacteremia, and septicemia, were resistant to multiple antibiotics including broad-spectrum cephalosporins. epidemiologic investigations revealed that the reservoir for these k. pneumoniae strains was the gastrointestinal tracts of the patients. the study of the adherence ability of the strains reported here showed that these bacteria adhered to the microvilli of the caco-2 cell li ... | 1992 | 1345909 |
| [postoperative infection control in patients with hepatic, biliary tract, and pancreatic cancers]. | postoperative infection occurs more frequently in patients with malignant disease than in patients with benign disease. postoperative infection control in patients with hepatic cancer, biliary tract cancer and pancreatic cancer is studied. although in patients with jaundice due to malignancy the rate of positive bacterial culture of the bile collected at the time of ptcd was low, the rate of positive bile culture increased after 10 to 14 days of ptcd. the predominant strain was enterococcus spp. ... | 1992 | 1346566 |
| fimbriation, capsulation, and iron-scavenging systems of klebsiella strains associated with human urinary tract infection. | thirty-two strains of klebsiella pneumoniae and seven strains of klebsiella oxytoca isolated from urinary tract infections in elderly adults were analyzed for capsular antigens, iron-scavenging systems, and fimbriation. all strains were capsulated. twenty-seven different k antigens were identified among the strains, with no particular antigen dominating. all strains produced the iron-scavenging system enterochelin as analyzed by bioassay and dna hybridization. in contrast, the aerobactin iron-se ... | 1992 | 1347287 |
| retrospective 6-year study of enterobacter bacteraemia in a danish university hospital. | in order to study the epidemiology of invasive enterobacter infections, data from 53 consecutive cases of bacteraemia due to this organism were compared with data from 72 randomly selected cases of escherichia coli bacteraemia. the cases occurred among patients admitted to a danish university hospital over a 6-year period. forty-eight cases were due to enterobacter cloacae and five were due to ent. aerogenes. enterobacter bacteraemia was more often of nosocomial origin than e. coli bacteraemia a ... | 1992 | 1348068 |
| adhesion of fimbriated and non-fimbriated klebsiella strains to synthetic polymers. | adherence of three fimbriated and non-fimbriated klebsiella strains to polyetherurethane was investigated in order to assess the possible role of fimbriae in the adhesion of klebsiella to synthetic polymers. fimbriated strains with type 1 and type 1.3 fimbriae adhere significantly stronger to polyurethane than the same strains lacking fimbriae, whereas a strain with type 3 fimbriae shows no different adherence compared with the non-fimbriated variant. analysis of adhesion kinetics and isotherms ... | 1992 | 1348437 |
| conserved immunoglobulin-like features in a family of periplasmic pilus chaperones in bacteria. | detailed structural analyses revealed a family of periplasmic chaperones in gram-negative prokaryotes which are structurally and possibly evolutionarily related to the immunoglobulin superfamily and assist in the assembly of adhesive pili. the members of this family have similar structures consistent with the overall topology of an immunoglobulin fold. seven pilus chaperone sequences from escherichia coli, haemophilus influenzae and klebsiella pneumoniae were aligned and their consensus sequence ... | 1992 | 1348692 |
| outbreak of infection in two uk hospitals caused by a strain of klebsiella pneumoniae resistant to cefotaxime and ceftazidime. | during an 8-month period, klebsiella pneumoniae resistant to cefotaxime and ceftazidime were isolated from 18 elderly patients in two closely-situated uk hospitals. amongst these 18 patients, the organisms were isolated from urine samples of 17, from blood cultures of two and from a wound swab of one. the infected patients were located in nine different wards and several of the patients had been transferred between wards, within and between the two hospitals. all the bacterial isolates belonged ... | 1992 | 1348768 |
| epidemiological role of arthropods detectable in health facilities. | a total of 161 arthropod specimens were collected from 55 sites in a health care facility during july and september 1990. of the 116 bacterial isolates obtained from their body surfaces 6% were from parasites (mosquitoes), 59% from eusynanthropic arthropods (tenebrionid beetles, flies, german cockroaches, wasps), 16% from hemisynanthropic arthropods (ants, spiders) and 19% from occasionally encountered insects (non-biting midges, moths, beetles). most (88%) of the isolated bacteria were gram-neg ... | 1992 | 1350604 |
| nitrogenase of klebsiella pneumoniae: an mgatp hydrolysing energy transduction system with similarities to actomyosin and p21 ras. | the mechanism of atp hydrolysis by nitrogenase shows some similarity to that proposed for actomyosin and for gtp hydrolysis by p21 ras. all three systems involve the formation of an active complex from two component proteins, nucleotide-induced changes in protein conformation, energy transduction that in the case of nitrogenase involves a decrease in redox potential of metal centres, and a slow dissociation of the protein complex. metal ion activation (mg2+ or ca2+) and in-line displacement of a ... | 1992 | 1351299 |
| microbiology of postoperative wound infection: a prospective study of 1770 wounds. | a prospective study of postoperative wound infection was carried out over a 12-month period. intra-operative swabs from the patients' anterior nares, the opened viscus and parietes were cultured using standard bacteriological techniques. of the 1770 wounds studied, 167 (9.4%) became infected. wound infection rates, according to clinical wound types, were clean 5.9%, clean-contaminated 10.7%, contaminated 24.3% and dirty 52.9%. the figures according to microbiological wound types were clean 4.7%, ... | 1992 | 1351494 |
| the potential neurotoxin 2-oh-dopamine is an inhibitor of arylsulfatase. | | 1992 | 1353330 |
| a study of 245 infected surgical wounds in singapore. | the aims of the study were to correlate the laboratory detection rate of wound infections with the actual wound infection rate, and to analyse the bacteriology of these wounds to provide a rationale for antibiotic usage in prophylaxis and treatment of surgical wound infections. the wound infection rate in a general surgical unit was determined using the most comprehensive surveillance available to us and was correlated with the laboratory detection rate. a correlation coefficient of 0.8 was obta ... | 1992 | 1353515 |
| pulo, a component of the pullulanase secretion pathway of klebsiella oxytoca, correctly and efficiently processes gonococcal type iv prepilin in escherichia coli. | the pulo protein required for extracellular secretion of pullulanase by klebsiella oxytoca is known to be highly homologous to two type iv prepilin peptidases, namely xcpa(pild) (pseudomonas aeruginosa) and tcpj (vibrio cholerae). the predicted prepilin peptidase activity of pulo was confirmed by showing that it could correctly process the product of the cloned pile.1 type iv pilin structural gene from neisseria gonorrhoeae in escherichia coli. the p. aeruginosa prepilin peptidase and another pu ... | 1992 | 1354833 |
| the rhizobium leguminosarum biovar phaseoli glnt gene, encoding glutamine synthetase iii. | plasmid pge203 contains the rhizobium leguminosarum biovar phaseoli glnt locus. glutamine synthetase iii (gsiii) was purified from a glutamine auxotrophic strain of klebsiella pneumoniae carrying this plasmid. sequencing of a 2.4-kb fragment containing the glnt locus reveals an open reading frame of 435 amino acids (aa), whose first eight aa are identical to those determined from pure gsiii by direct aa sequencing, thus confirming that glnt indeed codes for gsiii activity. the comparison of the ... | 1992 | 1356885 |
| activation of the rhizobium leguminosarum glnii gene by ntrc is dependent on upstream dna sequences. | the cloning and sequence determination is reported of the dna region of rhizobium leguminosarum coding for glutamine synthetase ii (gsii). an open reading frame (orf) encoding 326 amino acids was defined as the glnii gene on the basis of its similarity to other glnii genes and the ability of a dna fragment carrying this orf to complement the glutamine auxotrophy of a klebsiella pneumoniae glna mutant. we find that the glnii gene in r. leguminosarum is transcribed as a monocistronic unit from a s ... | 1992 | 1357539 |
| diversity of klebsiellae with extended-spectrum beta-lactamases at a turkish university hospital. | | 1992 | 1358959 |
| [the surface structures and biological properties of klebsiella strains]. | the electron-microscopic method has been used to study the surface structures and biological properties (adhesiveness, antibiotic-resistance, biochemical properties) in 67 strains of klebsiella isolated from one-year-old children with intestinal infections (56) and from healthy ones (11). it is found that bacterial cells in 58.2% of strains have fimbriae of the 3d type, in 20.9%--of the 1st type. no correlation has been revealed between the type of fimbriae and the studied properties. | 1992 | 1359388 |
| treatment of capd peritonitis with clavulanate potentiated ticarcillin. | a total of 16 episodes of peritonitis in 14 patients (9 males, 5 females), were treated with clavulanate potentiated ticarcillin (tc), a -lactamase stable parenteral penicillin. all the pts were hospitalized and received initial loading dose of 3.2 gr intraperitoneally (i.p.) in a 6-hour 1 l exchange, which was followed by four 1 l exchanges with 320 mg/ltc. the therapy was continued for ten days. the bacteria isolated were: staph. epid. (4), staph. aureus (2), strept. viridans (1), enterococcus ... | 1992 | 1361796 |
| isolation and characterization of type 1 fimbriae from a chicken pathogenic escherichia coli serotype o78. | type 1 fimbriae from chicken pathogenic escherichia coli strain pdi-386 (serotype o78) was purified and characterized. because of the acid-induced autoagglutination (t. sekizaki, y. nakasato, and i. nonomura, j. vet. med. sci. 54, 493-499, 1992), the fimbriae could be easily purified by repeating acid sedimentation, washing, and dissolving in buffer (ph 8.0). in electron microscopy, the purified fimbriae showed a filament of 8 nm in diameter and 10 microns in average length. the molecular mass o ... | 1992 | 1362082 |
| adult bacteremic pneumonia: bacteriology and prognostic factors. | bacteremic pneumonia is a highly specified subgroup of pneumonia that is potentially life-threatening. in order to find out the prognostic factors in this subgroup of pneumonia, we conducted a 40-month retrospective analysis of 70 cases in our hospital. the male to female ratio was 54:16. forty-one cases were community-acquired bacteremic pneumonia (cabp), and 29 cases were nosocomial bacteremic pneumonia (nbp). both cabp and nbp were predominated by gram-negative bacteria. klebsiella pneumoniae ... | 1992 | 1362112 |
| multiresistant klebsiella pneumoniae in a neonatal nursery: the importance of maintenance of infection control policies and procedures in the prevention of outbreaks. | during a 3-week period, nine babies in the neonatal unit of a large teaching hospital in durban were infected or colonized with klebsiella pneumoniae resistant to a range of antimicrobial agents including amikacin and cefotaxime. resistance to cefotaxime was reduced by clavulanic acid in vitro suggesting production of extended-spectrum beta-lactamase activity. all the isolates had the same antibiotic resistance profile, belonged to the same serotype (k17), were non-typable with bacteriophages, a ... | 1992 | 1362737 |
| pseudobacteraemia with multiply-resistant klebsiella pneumoniae resulting from contamination from the blood gas machine on a neonatal unit. | klebsiella pneumoniae with an unusual antibiotic susceptibility pattern was isolated from blood cultures of seven unwell premature babies on the special care baby unit. although the organism was sensitive to cefuroxime it was resistant to ceftazidime. it was also resistant to gentamicin, tobramycin, netilmicin, piperacillin and aztreonam but sensitive to ciprofloxacin, imipenem and amikacin. on extensive investigation to trace the source, a k. pneumoniae with the same susceptibility pattern as t ... | 1992 | 1362741 |
| [occurrence of p.fimbrii in strains from selected genera of enterobacteriaceae]. | this study was aimed at recognition of frequency of occurrence of p fimbriae in strains of escherichia coli isolated from samples of feces of children with symptoms of diarrhoea and at search of these adhesions in strains representing other than escherichia genera of enterobacteriaceae strains. one hundred forty laboratory strains were investigated. they belonged to genus citrobacter, enterobacter, hafnia, klebsiella, morganella, proteus, providencia, salmonella, shigella, and yersinia. also wer ... | 1992 | 1363960 |
| [panophthalmitis as a nosocomial infection]. | the submitted paper deals with an epidemic of severe postoperative panophthalmitis, its development, course, causes and sequelae incl. epidemiological characteristics. the disease developed in four patients 40 hours after operation of cataract. despite treatment all four patients developed septicaemia and therefore the affected eyeballs were eviscerated. from smears of the conjunctival sac of the affected patients and from the contents of the eviscerated eyeball proteus mirabilis and enterobacte ... | 1991 | 1364655 |
| lymphocyte proliferation and t-lymphocyte subsets during experimental immunomodulation of balb/c mice by olimunostim. | to objectivize possible immunomodulatory effects of polybacterial lysate olimunostim (p. acnes, k. pneumoniae, s. aureus), splenic lymphocyte proliferation and t-lymphocyte subsets were followed-up in balb/c mice administered perorally the lysate or saline (controls). the enhanced spontaneous lymphocyte proliferation observed at the beginning of olimunostim application preceded a gradual increase of lymphocyte reactivity to t-mitogens reaching the maximum five days after the administration of th ... | 1992 | 1364967 |
| study of modulatory effects of olimunostim on cellular immunity in healthy humans. | to objectivize potential modulatory effects of polybacterial lysate olimunostim on cellular immunity, the t-lymphocyte subpopulations and lymphocyte reactivity to mitogens were followed up in 10 healthy volunteers subjected to olimunostim application and parallelly in 6 healthy individuals receiving placebo. the preparation, which was applied perorally in seven consecutive daily doses, had no effects on t-lymphocytes and their subpopulations and exerted only a limited influence on lymphocyte rea ... | 1992 | 1364968 |
| sites of cadmium uptake in bacteria used for biosorption. | electron microscopy and x-ray spectroscopy were used to determine location and type of cadmium biosorption on and in bacteria, some of which produced extracellular polymers. examined arthrobacter and pseudomonas species appear to have detoxification systems that precipitate cadmium internally irrespective of whether or not they excrete polymers. capsular klebsiella aerogenes strains showed minimal intracellular uptake but over a 5-100 mg dm-3 cd range produced the highest net metal removal level ... | 1990 | 1366533 |
| recombinant brewer's yeast strains suitable for accelerated brewing. | four brewer's yeast strains carrying the alpha-ald gene of klebsiella terrigena (ex. aerobacter aerogenes) or of enterobacter aerogenes on autonomously replicating plasmids were constructed. the alpha-ald genes were linked either to the adc1 promoter or to the pgk1 promoter of yeast saccharomyces cerevisiae. in pilot scale brewing (50 l) with three of these recombinant yeasts the formation of diacetyl in beer was so low during fermentation that lagering was not required. all other brewing proper ... | 1990 | 1366907 |
| dna sequence of a salmonella-specific dna fragment and the use of oligonucleotide probes for salmonella detection. | hybridization specificity of a 1.8-kb hindiii dna fragment isolated from salmonella typhimurium by a molecular cloning technique was confirmed by colony hybridization with 327 salmonella isolates of various serotypes and 56 non-salmonella isolates including enterobacteriaceae closely related to salmonella, such as escherichia coli, klebsiella, citrobacter and shigella. it was found that this 1.8-kb dna fragment was highly specific for all the salmonella isolates tested. the dna sequence of this ... | 1991 | 1367316 |
| degradation of 3,4-dichloroaniline in synthetic and industrially produced wastewaters by mixed cultures freely suspended and immobilized in a packed-bed reactor. | degradation of 3,4-dichloroaniline (34dca) in aqueous solution by undefined cultures of free and immobilized cells was examined. batch cultures of freely suspended cells and continuous degradation in a packed-bed reactor were studied using both synthetically concocted and industrially produced waste-waters. 34dca was found to be degraded with a concomitant evolution of chloride ions into the bulk medium. the packed bed reactor with biomass immobilized on celite diatomaceous earth was found to be ... | 1991 | 1367396 |
| a hyperthermostable pullulanase produced by an extreme thermophile, bacillus flavocaldarius kp 1228, and evidence for the proline theory of increasing protein thermostability. | a cell-associated pullulanase (alpha-dextrin 6-glucanohydrolase, ec 3.2.1.41) of an extreme thermophile, bacillus flavocaldarius kp 1228, was purified to homogeneity. the molecular weight and isoelectric point were estimated to be about 55,000 and 7.0, respectively. the n-terminal sequence was ala-try-tyr-glu-gly-ala-phe-phe-tyr-gln-ile-phe-pro-asp-tyr-phe-phe-tyr- ala- gly-. the enzyme was most active at ph 6.3. the activities for 5% pullulan and 5% soluble starch were maximal at 75-80 degrees ... | 1991 | 1367521 |
| gene cloning of the maoa gene and overproduction of a soluble monoamine oxidase from klebsiella aerogenes. | we cloned the structural gene for monoamine oxidase (maoa) from klebsiella aerogenes into a pki212 vector in an maoa mutant strain of k. aerogenes. deletion analysis and complementation tests of the recombinant plasmid showed that the maoa gene was located entirely within a 4.1-kb segment. in an maoa mutant strain harbouring the cloned maoa gene, synthesis of monoamine oxidase was induced by addition of tyramine and related compounds. transfer of a plasmid containing the maoa gene into a monoami ... | 1991 | 1367577 |
| enzymatic synthesis of p-nitrophenyl alpha-maltoheptaoside by transglycosylation of maltohexaose-forming amylase. | an extracellular maltohexaose-forming amylase [ec 3.2.1.98] from klebsiella pneumoniae mutant is a normal hydrolytic enzyme that hydrolyzes short-chain amylose (dp = 23) to give about 40% maltohexaose. transglycosylation from maltoheptaose to the 4-position of p-nitrophenyl alpha-glucoside was efficiently induced through the use of maltohexaose-forming amylase in an aqueous methanol solution. the enzyme specifically produced p-nitrophenyl alpha-maltoheptaoside (13% of the p-nitrophenyl alpha-glu ... | 1990 | 1368529 |
| nifa protein synthesized in vitro binds to the upstream activator sequence in the promoter of the klebsiella oxytoca nifb gene. | | 1991 | 1368769 |
| dna amplification fingerprinting of bacteria. | we have amplified short arbitrary stretches of total bacterial dna to produce highly characteristic and complex dna fingerprints. this dna amplification fingerprinting (daf) strategy involves enzymatic amplification of dna directed by a single arbitrary oligonucleotide primer. amplification produces a characteristic spectrum of products that is adequately resolved by polyacrylamide gel electrophoresis and visualized by silver staining. although daf is simple in concept, we found that amplificati ... | 1992 | 1369011 |
| purification and characterization of nitrilase responsible for the enantioselective hydrolysis from acinetobacter sp. ak 226. | a nitrilase was purified, apparently to homogeneity, from a cell extract of acinetobacter sp. ak 226, which converts racemic 2-(4'-isobutylphenyl)propionitrile (ibu-cn) to s-(+)-2-(4'-isobutyl-phenyl)propionic acid (s-(+)-ibuprofen). the molecular weight of the native enzyme was estimated as 580,000 upon gel filtration. the nitrilase hydrolyzed many kinds of nitrile compounds such as aliphatic, aromatic, and heterocyclic mononitriles or dinitriles. the amino-terminal amino acids were sequenced a ... | 1991 | 1369128 |
| d-ribulokinase from klebsiella pneumoniae for continuous production of d-(-)-ribulose-5-phosphate. | the production of d-ribulose-5-phosphate in an enzyme membrane reactor was examined. phosphoryl transfer from atp to d-ribulose was catalysed by d-ribulokinase isolated from klebsiella pneumoniae. for production of d-ribulose-5-phosphate the phosphoryl donor atp was used either in stoichiometric or in catalytic amounts. using catalytic amounts of atp requires a second enzyme, e.g. pyruvate kinase, to regenerate atp. the kinetic parameters for d-ribulokinase and pyruvate kinase were determined to ... | 1990 | 1369262 |
| a general method to generate dna probes for microorganisms. | we present a method that permits the rapid generation of dna probes for eubacteria. in the procedure the variable regions for the 16s rrna genes are amplified using polymerase chain reaction (pcr) technology and primers based on the conserved regions of these genes. following sequencing of the variable regions, a choice is possible for a probe specific for that organism. no knowledge of the molecular biology of the microorganism is required prior to the application of this approach. the generali ... | 1990 | 1369980 |
| production of cyclodextrins, a novel carbohydrate, in the tubers of transgenic potato plants. | cyclodextrins (cds) are cyclic oligosaccharides containing six (alpha), seven (beta), or eight (gamma) glucose molecules, respectively. the cyclodextrin glycosyltransferases (cgt), which produce cds from starch, are found only in bacteria and are used in batch fermentors with hydrolyzed starch to produce cds commercially. using a cgt gene from klebsiella, we attempted to engineer the tubers of developing potatoes to produce these novel, high-value carbohydrates. a chimeric gene, consisting of (1 ... | 1991 | 1370622 |
| [respiratory infections associated with lung cancer]. | we have analyzed the clinical significance of secondary infections associated with lung cancer patients. the incidence of secondary infections was 51.4% in 214 in-patients with lung cancer admitted to our institution in 1988 and 1989, and almost all of them had respiratory tract infections. the incidence was high in patients with cell types other than adenocarcinoma, and in those with hypoproteinemia, impaired cellular immunity and obstruction of the airway. the prognosis in patients with infect ... | 1992 | 1371046 |
| mapping and characterization of the promoter elements of the regulatory nif genes rpon, nifa1 and nifa2 in rhodobacter capsulatus. | the promoter elements responsible for the expression of the regulatory nif genes rpon, nifa1 and nifa2 of rhodobacter capsulatus were mapped by exonuclease-iii-mediated deletions and by primer extension analysis. the rpon promoter maps 600 bp upstream of rpon and has the characteristic features of a -24/-12 promoter. the upstream activator sequence (uas) displays two mismatches with the nifa consensus sequence and is located 37 bp upstream of a perfect -24/-12 promoter element. the spacing and/o ... | 1992 | 1374827 |
| purification of a distinctive form of endotoxin-induced nitric oxide synthase from rat liver. | an endotoxin-induced form of nitric oxide synthase (ec 1.14.23) was purified to homogeneity from rat liver by sequential anion-exchange chromatography and affinity chromatography using 2',5'-adp-sepharose. the enzyme has a subunit molecular mass of 135 kda as determined by sds/page, a maximum specific activity of 462 nmol of citrulline formed from arginine per min per mg, and a km for arginine of 11 microm. the enzyme was strongly stimulated by the addition of calmodulin with an ec50 of 2 nm, bu ... | 1992 | 1376917 |
| increased mutagenicity of some nitroimidazoles by non-mutagenic nitrotoluene on klebsiella pneumoniae (fluctuation test). | in the fluctuation test the mutation frequency of klebsiella pneumoniae by the 5-nitroimidazoles panidazole and dimetridazole was increased by adding the non-mutagenic substances 4-nitrotoluene or toluene-4-sulfonamide. this effect was not found with 1-methyl-5-nitroimidazole, metronidazole, ronidazole, nimorazole and 1-methyl-2-hydroxymethyl-5-nitroimidazole. it is suggested that the molecules of panidazole or dimetridazole form some association, which is destroyed by 4-nitrotoluene or toluene- ... | 1992 | 1377354 |
| ru 41.740 triggers human mononuclear blood cells to release tumor growth inhibitory factors in vitro. | ru 41.740 (biostim) is an immunostimulating drug of microbial origin which may stimulate human mononuclear blood cells (mainly monocytes) to release soluble factors which inhibit replication of several tumor cell lines in vitro. since this effect may be of clinical importance in the treatment of cancer a number of tests have been conducted in order to find methods to augment this secretion. in vitro tests suggested that this non-specific antitumor activity of biostim may not be enhanced by conco ... | 1992 | 1378044 |
| identification of acor, a regulatory gene for the expression of genes essential for acetoin catabolism in alcaligenes eutrophus h16. | two hundred thirty-nine base pairs upstream from acoxabc, which encodes the alcaligenes eutrophus h16 structural genes essential for cleavage of acetoin, the 2,004-bp acor gene was identified. acor encodes a protein of 668 amino acids with a molecular mass of 72.9 kda. the amino acid sequence deduced from acor exhibited homologies to the primary structures of transcriptional activators such as nifa of azotobacter vinelandii, ntrc of klebsiella pneumoniae, and hoxa of a. eutrophus. striking simil ... | 1992 | 1378052 |
| molecular cloning of the rfb region of klebsiella pneumoniae serotype o1:k20: the rfb gene cluster is responsible for synthesis of the d-galactan i o polysaccharide. | previous chemical analyses identified two structurally distinct o polysaccharides in the lipopolysaccharide of klebsiella pneumoniae serotype o1:k20 (c. whitfield, j. c. richards, m. b. perry, b. r. clarke, and l. l. maclean, j. bacteriol. 173:1420-1431, 1991). the polysaccharides were designated d-galactan i and d-galactan ii; both are homopolymers of galactose. to begin investigation of the synthesis and expression of these o polysaccharides, we have cloned a 7.3-kb region of the chromosome of ... | 1992 | 1378055 |
| structural analysis of the o-antigen side chain polysaccharides in the lipopolysaccharides of klebsiella serotypes o2(2a), o2(2a,2b), and o2(2a,2c). | the lipopolysaccharide (lps) of klebsiella serotype o2 is antigenically heterogeneous; some strains express multiple antigenic factors. to study this heterogeneity, we determined the structure of the o-antigen polysaccharides in isolates belonging to serotypes o2(2a), o2(2a,2b), and o2(2a,2c), by using composition analysis, methylation analysis, and both 1h and 13c nuclear magnetic resonance spectroscopy. the repeating unit structure of the 2a polysaccharide was identified as the disaccharide [- ... | 1992 | 1378428 |
| [combination effect of kw-2228 and cephem antibiotics in a systemic infection model in neutropenic mice]. | a modified recombinant human granulocyte colony-stimulating factor (rhg-csf), kw-2228, has some excellent properties such as high specific activity in stimulating granulocyte colony-formation in vitro, great biological stability in plasma, good pharmacokinetic profile and high potency in granulopoiesis in normal mice in vivo. recently, the application of g-csf against infectious diseases has been considered, and some animal experiments have been carried out to support its clinical applications. ... | 1992 | 1379649 |
| [immunobiological properties and therapeutic effectiveness of preparations from klebsiella bacteriophages]. | the purified preparations of klebsiella bacteriophages, viz. the monovalent preparation of k. pneumoniae bacteriophage and the polyvalent bacteriophage preparation for the treatment of infections caused by k. ozaenae, k. rhinoscleromatis scleromatis and k. pneumoniae sensu lato, have been obtained. the bacteriophage preparations have proved to be nontoxic and safe for laboratory animals after the intraperitoneal injection of these preparations followed by the pathomorphological study of the inte ... | 1992 | 1380753 |
| cloning and properties of a cyanide hydratase gene from the phytopathogenic fungus gloeocercospora sorghi. | the cht gene encoding cyanide hydratase (cht, ec 4.2.1.66), which detoxifies hcn and is thought to be important in fungal infection of cyanogenic plants, has been cloned from the phytopathogenic fungus gloeocercospora sorghi. the gene was isolated by screening an expression library of g. sorghi using a cht-specific antibody and using one of the positive cdna clones as a probe in southern hybridization to identify a 3.1 kb psti genomic fragment. this psti fragment expressed cht activity when tran ... | 1992 | 1382413 |
| reactivity of monoclonal antibody e5 with endotoxin. ii. binding to short- and long-chain smooth lipopolysaccharides. | the murine monoclonal igm antibody e5 has been shown to significantly reduce the mortality and morbidity of patients with gram-negative sepsis in a multicenter randomized placebo-controlled clinical trial. the in vitro binding characteristics of monoclonal antibody (mab) e5 were studied using highly purified smooth lipopolysaccharide (lps) isolated from a variety of clinically relevant, wild-type gram-negative bacteria. using a sensitive antibody-capture assay which involves immobilized mab e5 a ... | 1992 | 1382882 |
| novel adjuvant action of lipopolysaccharides that possess mannose homopolysaccharides as o-specific polysaccharides on immune responses to nonimmunogenic autoantigens in mice. | the adjuvant action of various lipopolysaccharides on immune responses to syngeneic tissue extract in mice was examined. only lipopolysaccharides possessing the linear mannose homopolysaccharides as o-specific polysaccharides exhibited definite adjuvant action on immune responses to the autoantigens. the intensity of this adjuvant activity of lipopolysaccharide from klebsiella o3 seemed to be the strongest. | 1992 | 1383160 |
| ribosomes as carriers for antigenic determinants of the surface of micro-organisms. | over the past twenty-five years, many authors have reported evidence of the immunoprotective capacity of ribosomes isolated from bacteria, fungi and parasites. since 1971 we have explored the protective capacity of ribosomes isolated from a large variety of micro-organisms responsible for human and animal diseases. accurate biochemical characterization of ribosomes always reveals trace amounts of non-ribosomal components such as short polysaccharides strongly linked to ribosomal rna after phenol ... | 1992 | 1385236 |
| [plasmid profile and toxicity of klebsiella pneumoniae strains isolated at a neonatal department]. | the authors tested the plasmid profile of 11 and the toxicity of 16 strains of k. pneumoniae isolated in a neonatal department. based on examination of the plasmid profile it may be assumed that very probably a nosocomial spread of the strains was involved. this is suggested by the identical plasmid profile of all strains isolated in the environment and five strains isolated from neonates. on investigation of the enterotoxicity of culture filtrates of the investigated strains the authors did not ... | 1992 | 1388102 |
| incidence of two virulence factors (aerobactin and mucoid phenotype) among 190 clinical isolates of klebsiella pneumoniae producing extended-spectrum beta-lactamase. | because outbreaks of multiple-resistant klebsiella pneumoniae isolates producing extended-spectrum beta-lactamases were recently observed in french hospitals, the presence of virulence factors was examined for (i) phenotype by bioassay for aerobactin production and by culture for the mucoid phenotype, and (ii) genotype using intragenic probes of respectively 2-kb bglii and 235-bp bamhi-bglii fragments and dot-blotting among 190 unreplicated k. pneumoniae clinical isolates issued from 25 french h ... | 1992 | 1388129 |
| mechanistic and structural studies on rhodococcus atcc 39484 nitrilase. | rhodococcus atcc 39484 produced a nitrilase when induced with isovaleronitrile. the enzyme was obtainable pure in milligram amounts, had a subunit mr of 40 kda, and demonstrated a substrate-induced activation related to aggregation of subunits to form a 560-kda complex. the enzyme had a broad substrate specificity, had a ph optimum of 7.5, was stable up to 40 degrees c, and had one disulfide bridge and two free cysteine residues, one of which appeared to be catalytically essential. the n-termina ... | 1992 | 1388821 |
| primary structure of an aliphatic nitrile-degrading enzyme, aliphatic nitrilase, from rhodococcus rhodochrous k22 and expression of its gene and identification of its active site residue. | peptides obtained by cleavage of a rhodococcus rhodochrous k22 nitrilase, which acts on aliphatic nitriles such as acrylonitrile, crotonitrile, and glutaronitrile, have been sequenced. the data allowed the design of oligonucleotide probes which were used to clone a nitrilase encoding gene. plasmid pnk21, in which 2.05-kb sequence covering the region encoding the nitrilase was was placed under the control of the lac promoter, directed overproduction of enzymatically active nitrilase in response t ... | 1992 | 1390687 |
| brain abscess in infants. | brain abscesses are rare in infants and their clinical presentation is specific for this age group. seven cases of brain abscess in infants aged 2-11 months are reported. the underlying cause was meningitis in four, sepsis in two, and unknown in one. gram-negative organisms were cultured in 6 patients. the abscess size was 5 cm or more in five cases; in four there were multiple lesions. two abscesses were aspirated and irrigated; four particularly large lesions were drained and repeatedly aspira ... | 1992 | 1394267 |
| re-investigation of the structure of the capsular polysaccharide of klebsiella k15 using bacteriophage degradation and inverse-detected nmr experiments. | the structure of the capsular polysaccharide from klebsiella k15 has been re-investigated, principally by 1d and 2d 1h- and 13c-nmr spectroscopy of the oligosaccharide-alditol obtained by depolymerisation of the polysaccharide with a viral-borne endoglycanase followed by borohydride reduction of the isolated repeating oligosaccharide. the capsular polysaccharide was shown to have the repeating unit: [formula: see text] | 1992 | 1394332 |
| [bacterial contamination of arthropods in a health facility]. | at 55 sites of a health institution in july and september 1990 a total of 161 specimens of arthropods were detected, 30 outdoors and 131 on the premises of the health institution. on their bodies 116 bacterial strains were isolated, mostly gram-negative rods (more than 85%), in particular spp. enterobacter, acinetobacter, klebsiella, citrobacter and pseudomonas. gram-positive cocci accounted for cca 12%, in particular strains of s. haemolyticus and s. hominis. the greatest number of strains was ... | 1992 | 1394473 |