| analysis of the microbiota of sputum samples from patients with lower respiratory tract infections. | sputum is the most common sample collected from patients suffering from lower respiratory tract infections and it is crucial for the bacterial identification of these infections. in this study, we enrolled 101 sputum samples from 101 patients with lower respiratory tract infections. initially, pyrosequencing of the 16s rdna v3 hypervariable regions of the bacteria contained in the sputum was utilized as a culture-independent approach for microbiota analysis. for comparison, clinical laboratory t ... | 2010 | 20823075 |
| protean neuroimaging presentations in an adult with klebsiella pneumoniae infection. | to report on the findings of a series of cranial computed tomography (ct) and magnetic resonance (mr) imaging studies of a 57-years-old woman with klebsiella (k.) pneumoniae infection. | 2010 | 20824541 |
| [renal and urethral gas production as a complication to infection of the urinary tract]. | emphysematous pyelonephritis (epn) is a bacterial infection characterised by gas production in the renal parenchyma. we present a case with epn caused by klebsiella pneumoniae sepsis. the 74-year-old woman was admitted in septic shock. an acute computed tomography revealed gas bubbles bilaterally in the renal parenchyma, left urethra and bladder. treatment consisted of three types of antibiotics and intravenous fluids. she was discharged two months later without need for surgical treatment or ha ... | 2010 | 20825745 |
| [extended-spectrum beta-lactamase producing-enterobacteriaceae]. | extended-spectrum beta-lactamase (esbls) are defined as β-lactamase capable of hydrolyzine oximino-cephalosporins and aztreonam and are encoded by mobile genes. the most frequently encountered esbls belong to the ctx-m, shv, and tem families. esbls were found first in klebsiella pneumonia and then predominantly in e. coli. the incidence of patients with esbls e. coli increase since 2000 in robert debré hospital. they were responsible of cystitis or pyelopnephritis and rarely of materno-foetal in ... | 2010 | 20826322 |
| [description and investigation of an outbreak of extended-spectrum beta-lactamase producing escherichia coli strain in a neonatal unit]. | an outbreak of colonization and infection with an escherichia coli strain producing extended-spectrum beta-lactamase (esbl) occurred in a neonatal unit : a high rate of cases was observed, 27/59 neonates were colonized : one of them developed meningitis with favourable outcome and another baby developed conjunctivitis. despite intensive efforts to control the outbreak by standard methods of hand hygiene, patients screening and isolation, the spread was uncontrolled and the unit was closed to all ... | 2010 | 20826323 |
| [implication of extended-spectrum beta-lactamase enterobacteriaceae in nosocomial infections in neonates]. | the objective of this study was to determine the incidence of extended-spectrum beta-lactamase (esbls) enterobacteriaceae colonization and infection in hospitalized children. | 2010 | 20826324 |
| [carbapenemases in enterobacteriaceae]. | carbapenem-hydrolyzing beta-lactamases are the most powerful mechanism of resistance to carbapenems. carbapenemases have been reported extensively worldwide now in enterobacteriaceae. carbapenemases of the kpc type have been reported first from the usa in klebsiella pneumoniae, then worldwide with a marked endemicity in israel and greece. metallo-enzymes (vim, imp…) have been also reported internationnaly with high prevalence in southern europe and asia. oxa-48 which is one of the latest carbape ... | 2010 | 20826325 |
| [epidemiology of urinary tract infection in children in marrakech]. | by its incidence, the polymorphism of its clinical presentation, its potential gravity, the difficulty of realization of urine culture in children, the frequency of the subjacent urologic abnormalities, the urinary tract infection (uti) is an important problem in paediatrics. this work relates to a retrospective study carrying 121 episodes of uti in 111 children taken care in paediatrics services of the teaching hospital mohammed vi in marrakech between march 2005 and march 2009. the age of our ... | 2010 | 20826328 |
| structural and mechanistic studies on klebsiella pneumoniae 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase. | the stereospecific oxidative degradation of uric acid to (s)-allantoin was recently shown to proceed via three enzymatic steps. the final conversion is a decarboxylation of the unstable intermediate 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline (ohcu) and is catalyzed by ohcu decarboxylase. here we present the structures of klebsiella pneumoniae ohcu decarboxylase in unliganded form and with bound allantoin. these structures provide evidence that ligand binding organizes the active site residues ... | 2010 | 20826786 |
| isolation of mutant alginate lyases with cleavage specificity for di-guluronic acid linkages. | alginates are commercially valuable and complex polysaccharides composed of varying amounts and distribution patterns of 1-4-linked β-d-mannuronic acid (m) and α-l-guluronic acid (g). this structural variability strongly affects polymer physicochemical properties and thereby both commercial applications and biological functions. one promising approach to alginate fine structure elucidation involves the use of alginate lyases, which degrade the polysaccharide by cleaving the glycosidic linkages t ... | 2010 | 20826807 |
| occurrence and antibiotic resistance of coliform bacteria and antimicrobial residues in pasteurized cow's milk from brazil. | in this study we examined the hygienic and sanitary quality of pasteurized cow's milk in the state of paraná, brazil, by determining the presence of coliforms and occurrence of antimicrobial residues. a total of 260 milk samples were collected from commercial establishments in different regions of the state. coliform populations were estimated by the multiple-tube test, and antimicrobial residues were detected by enzyme-linked immunosorbent assay. overall, 105 samples (40.4%) were unsuitable for ... | 2010 | 20828476 |
| [fecal carriage of third generation cephalosporins resistant enterobacteteriaceae in asymptomatic young adults: evolution between 1999 and 2009]. | the aim of this work was to evaluate the fecal carriage of third generation cephalosporins resistant enterobacteriaceae in nonhospitalized asymptomatic young adults. | 2011 | 20828938 |
| molecular ecology and polyphasic characterization of the microbiota associated with semi-dry processed coffee (coffea arabica l.). | this work was aimed at isolating and identifying the microbiota present during the semi-dry method of coffee processing using polyphasic methods and to evaluate microbial diversity with pcr-dgge. samples of coffea arabica l. were collected during different processing stages in southern minas gerais, brazil. the bacterial and fungal isolates were phenotypically characterised and grouped according to the ardra technique, in which the 16-23s and its1-5.8s regions of the rdna were sequenced for spec ... | 2010 | 20832694 |
| enterobacteriaceae act in concert with the gut microbiota to induce spontaneous and maternally transmitted colitis. | disruption of homeostasis between the host immune system and the intestinal microbiota leads to inflammatory bowel disease (ibd). whether ibd is instigated by individual species or disruptions of entire microbial communities remains controversial. we characterized the fecal microbial communities in the recently described t-bet(-/-) ×rag2(-/-) ulcerative colitis (truc) model driven by t-bet deficiency in the innate immune system. 16s rrna-based analysis of truc and rag2(-/-) mice revealed distinc ... | 2010 | 20833380 |
| synthesis and in vitro antibacterial activity of fluoroquinolone derivatives containing 3-(n'-alkoxycarbamimidoyl)-4-(alkoxyimino) pyrrolidines. | a series of novel 7-[3-(n'-alkoxycarbamimidoyl)-4-(alkoxyimino)pyrrolidin-1-yl] fluoroquinolone derivatives were designed, synthesized and characterized by (1)h nmr, ms and hrms. these fluoroquinolones were screened for their in vitro antibacterial activity. most of them exhibit good potency in inhibiting the growth of staphylococcus aureus and staphylococcus epidermidis (mic: 0.06-4.00 μg/ml). the activity of compounds 33 and 43 against s. aureus including mrsa and s. epidermidis including mrse ... | 2010 | 20833454 |
| endotoxin does not alter the pharmacokinetics of micafungin, but it impairs biliary excretion of micafungin via multidrug resistance-associated protein 2 (abcc2/mrp2) in rats. | micafungin, a newly developed echinocandin-type antifungal agent, is widely used for the treatment of deep-seated fungal infections including those of candida species and aspergillus species. in the present study, the possible alterations in the pharmacokinetics and biliary excretion of micafungin were investigated in endotoxemic rats induced by klebsiella pneumoniae endotoxin. endotoxin (2 mg/kg) was injected intraperitoneally 24 h before an intravenous injection of micafungin (1 mg/kg). no sig ... | 2011 | 20835743 |
| [acquired bacterial meningitis in monastir region, tunisia (1999-2006): bacteriological aspects and susceptibility patterns]. | the aim of this study was to analyze the distribution of bacteria responsible for purulent meningitis and the pattern of resistance of common species in the university hospital of monastir (tunisia). all bacteriologically confirmed cases of bacterial meningitis were recorded between 1999 and 2006, and have been analyzed by classic bacterial methods advocate for meningitis. two hundred fifty three strains have been isolated. the most frequent species were streptococcus pneumoniae, haemophilus inf ... | 2011 | 20835902 |
| infections in polymyositis and dermatomyositis: analysis of 192 cases. | to estimate the incidence, characteristics and predictors of infections in patients with pm and dm. | 2010 | 20837496 |
| preliminary phytochemical screening and antimicrobial studies of lantana indica roxb. | the aim of the present study was to investigate the antimicrobial and preliminary phytochemical properties of lantana indica roxb. the aqueous and organic solvent (ethyl acetate and methanol) extracts from the leaves of lantana indica (verbenaceae) were tested against staphylococcus aureus, bacillus subtilis, steptococcus pyrogens, escherichia coli, proteus vulgaris, klebsiella pneumoniae, pseudomonas aeruginosa, salmonella typhi, aspergillus niger and candida albicans by agar well diffusion met ... | 2010 | 20838528 |
| bacterial contamination of donor blood at the tamale teaching hospital, ghana. | transfusion of bacterially contaminated blood can result in sepsis and will constitute a substantial health burden to the patient. | 2009 | 20842237 |
| the in vitro antimicrobial activity of fruit and leaf crude extracts of momordica charantia: a tanzania medicinal plant. | to evaluate the antimicrobial activity of momordica charantia extracts on reference strains and microorganisms isolated from clinical specimens. | 2009 | 20842240 |
| antibiotic susceptibility pattern in urinary isolates of gram negative bacilli with special reference to ampc β-lactamase in a tertiary care hospital. | resistance to higher antimicrobial agent is commonly seen in gram negative bacilli. this issue is a challenging problem to the medical practitioners in addition to it is financial impact on the health care system. | 2010 | 20842250 |
| recurrent urinary tract infections in women: diagnosis and management. | recurrent urinary tract infections, presenting as dysuria or irritative voiding symptoms, are most commonly caused by reinfection with the original bacterial isolate in young, otherwise healthy women with no anatomic or functional abnormalities of the urinary tract. frequency of sexual intercourse is the strongest predictor of recurrent urinary tract infections in patients presenting with recurrent dysuria. in those who have comorbid conditions or other predisposing factors, recurrent complicate ... | 2010 | 20842992 |
| carbapenem heteroresistance in vim-1-producing klebsiella pneumoniae isolates belonging to the same clone: consequences for routine susceptibility testing. | susceptibility results with low reproducibility by the same or different methods have been observed for metallo-beta-lactamase (mbl)-producing enterobacteriaceae. eighteen vim-1-producing klebsiella pneumoniae isolates (one per patient) belonging to a single epidemic clone in our hospital (2005 to 2008) but with different susceptibilities to carbapenems were studied. imipenem mics ranged from 8 to >128 mg/liter by standard clsi microdilution, from ≤1 to >8 mg/liter by the semiautomatic wider sys ... | 2010 | 20844213 |
| klebsiella pneumoniae subverts the activation of inflammatory responses in a nod1-dependent manner. | klebsiella pneumoniae is an important cause of community-acquired and nosocomial pneumonia. subversion of inflammation is essential for pathogen survival during infection. evidence indicates that k. pneumoniae infections are characterized by lacking an early inflammatory response although the molecular bases are currently unknown. here we unveil a novel strategy employed by a pathogen to counteract the activation of inflammatory responses. k. pneumoniae attenuates pro-inflammatory mediators-indu ... | 2011 | 20846183 |
| a novel mutation in the connexin 26 gene (gjb2) in a child with clinical and histological features of keratitis-ichthyosis-deafness (kid) syndrome. | keratitis-ichthyosis-deafness (kid) syndrome is a rare congenital ectodermal disorder, caused by heterozygous missense mutation in gjb2, encoding the gap junction protein connexin 26. the commonest mutation is the p.asp50asn mutation, and only a few other mutations have been described to date. | 2011 | 20846357 |
| ctx-m-producing klebsiella spp. in a brazilian hospital: what has changed in 6 years? | ctx-m-encoding genes from klebsiella spp. strains isolated in 2000 and 2006 were characterized as well as their genetic environment. ctx-m-2 variants were predominant in klebsiella pneumoniae strains, which showed a greater variability in bla(ctx-m) genes, integrons, and plasmids in 2006 when compared to strains collected in 2000. ctx-m-9-producing klebsiella oxytoca was identified in 2000 as clonal dissemination. | 2010 | 20846594 |
| salvage therapy with tigecycline for recurrent infection caused by ertapenem-resistant extended-spectrum β-lactamase-producing klebsiella pneumoniae. | optimal antimicrobial therapy for infections due to ertapenem-resistant enterobacteriaceae remains undetermined. in this study, a diabetic patient with recurrent pyomyositis and osteomyelitis caused by extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae developed ertapenem resistance after imipenem/cilastatin treatment, which was a currently recommended therapy. he was finally treated successfully using tigecycline. ertapenem resistance was in part explained by the production of ... | 2010 | 20846813 |
| high frequency of beta-lactam susceptibility in ctx-m-type extended-spectrum-beta-lactamase-producing klebsiella pneumoniae, escherichia coli and proteus mirabilis according to the new clsi recommendations. | | 2010 | 20846935 |
| detection of tem & shv genes in escherichia coli & klebsiella pneumoniae isolates in a tertiary care hospital from india. | extended spectrum beta lactamases (esbls) have been observed in virtually all the species of family enterobacteriaceae. the enzymes are predominantly plasmid mediated and are derived from broad-spectrum beta lactamase tem-1, tem-2 or shv-1 by a limited number of mutations. this study was undertaken to characterize esbl producers among escherichia coli and klebsiella pneumoniae by pcr-rflp, which were initially screened by phenotypic method. | 2010 | 20847381 |
| risk factors for hospital-acquired urinary tract infection: a case-control study. | the objective is to assess risk factors and microbiological aspects of hospital-acquired urinary tract infection (hauti) on six wards of a general regional hospital in serbia. a case-control study was nested within prospective cohort hautis study conducted from january 1 to december 31, 2007. three controls were identified for each patient with hauti, being chronologically the next three patients surveyed who did not develop hauti. the patients and controls were matched by sex and age (±5 years) ... | 2011 | 20848199 |
| new delhi metallo-beta lactamase (ndm-1) in enterobacteriaceae: treatment options with carbapenems compromised. | carbapenems are among the few useful antibiotics against multidrug resistant gram negative bacteria particularly those with extended spectrum beta lactamase. however resistance to carbapenems occurs and is mediated by mechanisms like loss of outer membrane proteins and production of beta lactamase that is capable of hydrolyzing carbapenems. an alert issued in the uk in 2009 warned of an increasing number of carbapenem resistant enterobacteriaceae strains identified in uk hospital patients. many ... | 2010 | 20848811 |
| seasonal and ascending trends in the incidence of carriage of extended-spectrum ß-lactamase-producing escherichia coli and klebsiella species in 2 german hospitals. | extended-spectrum ß-lactamase (esbl)-producing strains of bacteria have become a major public health concern. in the present study, the incidence of carriage of esbl-producing strains was analyzed for general trends and seasonality. | 2010 | 20849274 |
| antimicrobial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | the aim of this study is to assess the antibacterial activity of sodium citrate against streptococcus pneumoniae and several oral bacteria. | 2010 | 20849395 |
| [clinical characteristics of neonatal klebsiella pneumoniae sepsis and the antibiotic sensitivity pattern of strains]. | to study the clinical characteristics of neonatal sepsis caused by klebsiella pneumoniae and the antibiotic sensitivity pattern of klebsiella pneumoniae strains. | 2010 | 20849717 |
| the diversilab system versus pulsed-field gel electrophoresis: characterisation of extended spectrum β-lactamase producing escherichia coli and klebsiella pneumoniae. | fast and reliable epidemiological typing methods for identifying outbreaks and epidemic strains of extended spectrum β-lactamase (esbl) producing enterobacteriaceae are urgently needed. the diversilab system (dl) has been proposed for these purposes. we compared dl to pulsed-field gel electrophoresis (pfge) on a national collection of esbl-producing escherichia coli (n=258; of which 226 isolates were typeable with pfge) and klebsiella pneumoniae (n=48) isolated in 2007. for e. coli the wallace c ... | 2010 | 20849889 |
| chemoenzymatic synthesis, inhibition studies, and x-ray crystallographic analysis of the phosphono analog of udp-galp as an inhibitor and mechanistic probe for udp-galactopyranose mutase. | udp (uridine diphosphate) galactopyranose mutase (ugm) is involved in the cell wall biosynthesis of many pathogenic microorganisms. ugm catalyzes the reversible conversion of udp-α-d-galactopyranose into udp-α-d-galactofuranose, with the latter being the precursor of galactofuranose (galf) residues in cell walls. glycoconjugates of galf are essential components in the cell wall of various pathogenic bacteria, including mycobacterium tuberculosis, the causative agent of tuberculosis. the absence ... | 2010 | 20850454 |
| cmy-2, cmy-8b, and dha-1 plasmid-mediated ampc β-lactamases among clinical isolates of escherichia coli and klebsiella pneumoniae from a university hospital, thailand. | between february 2005 and january 2006 in srinagarind hospital, thailand, 44 from 1730 isolates (2.5%) of escherichia coli and 8 from 982 isolates (0.8%) of klebsiella pneumoniae were found to produce plasmid-mediated ampc β-lactamases (pampcs) as detected by a cefoxitin-hodge test followed by a multiplex polymerase chain reaction (pcr) technique. fifteen of the 52 pampc-producing isolates also produced extended-spectrum β-lactamases. the ampc genes found in both organisms were bla(cmy-2) (46 is ... | 2010 | 20851550 |
| detection of kpc-2-producing citrobacter freundii isolates in spain. | | 2010 | 20851814 |
| prevalence and resistance patterns of extended-spectrum and ampc β-lactamase in escherichia coli, klebsiella pneumoniae, proteus mirabilis, and salmonella serovar stanley in a korean tertiary hospital. | a total of 100 clinical isolates of escherichia coli (n = 35), klebsiella pneumoniae (n = 63), proteus mirabilis (n = 1), and salmonella serovar stanley (n = 1), showing resistance to cefoxitin, or returning positive in extended-spectrum β-lactamase (esbl) by clinical and laboratory standards institute (clsi) esbl confirmatory method, were studied. the isolates were examined by the boronic acid (ba) disk test, polymerase chain reaction, and pulsed-field gel electrophoresis (pfge) to investigate ... | 2010 | 20854475 |
| clinical disease caused by klebsiella in 2 unrelated patients with interleukin 12 receptor beta1 deficiency. | patients with interleukin 12 (il-12)p40 or il-12 receptor β1 (il12rβ1) deficiencies are prone to develop infections caused by mycobacteria and salmonella; other infections have only been rarely observed. in this report we describe 2 unrelated patients with complete autosomal recessive il12rβ1 deficiency who suffered from sepsis attributable to klebsiella pneumoniae. a mexican boy suffered from disseminated bacille calmette-guérin disease and infections caused by k pneumoniae and candida albicans ... | 2010 | 20855390 |
| microbiologic and clinical implications of bacteremia due to extended-spectrum-beta-lactamase-producing klebsiella pneumoniae with or without plasmid-mediated ampc beta-lactamase dha-1. | bacteremias caused by klebsiella pneumoniae producing extended-spectrum beta-lactamase (esbl-kp; n = 52) and producing both esbl and ampc-type dha-1 beta-lactamase (esbl-pmabl-kp; n = 20) were analyzed. higher mic(50)s and mic(90)s for carbapenems, ciprofloxacin, and piperacillin-tazobactam were observed with esbl-pmabl-kp than with esbl-kp. patients with oxyimino-β-lactam exposure and high modified pitt bacteremia scores (hmpbss) were at higher risk, while those with piperacillin-tazobactam and ... | 2010 | 20855742 |
| antibiotic optimization in the difficult-to-treat patient with complicated intra-abdominal or complicated skin and skin structure infections: focus on tigecycline. | complicated intra-abdominal and skin and skin structure infections are widely varied in presentation. these infections very often lead to an increase in length of hospital stay, with a resulting increase in costs and mortality. in addition, these infections may be caused by a wide variety of bacteria and are often polymicrobial with the possibility of the presence of antimicrobial-resistant strains, such as methicillin-resistant staphylococcus aureus, vancomycin-resistant enterococci, extended-s ... | 2010 | 20856688 |
| deep neck abscesses: the singapore experience. | this study aims to review our experience with deep neck abscesses, identify key trends, and improve the management of this condition. this is a retrospective chart review of patients diagnosed with deep neck abscesses in the department of ent (otorhinolaryngology) at tan tock seng hospital, singapore between 2004 and 2009. patient demographics, etiology, bacteriology, systemic disease, radiology, treatment, complications, duration of hospitalization, and outcomes were reviewed. 131 patients were ... | 2011 | 20857130 |
| prevalence of bacteria and parasites in white ibis in egypt. | a field survey was conducted to evaluate the prevalence of bacterial infections among free-living white ibis (nipponia nippon) in which 92 bacterial isolates were recovered from 193 different internal organs of 55 apparently healthy ibis. escherichia coli and salmonella spp. were isolated at rates of 43.6% and 14.5%, respectively. the other bacterial pathogens isolated were shigella spp. (34.5%), enterobacter spp. (21.8%) citrobacter spp. (18.1%), klebsiella pneumonia (16.3), staphylococcus aure ... | 2010 | 20857378 |
| [carbapenem-resistant klebsiella pneumoniae following foreign travel]. | this is the first report of 3 patients in whom carbapenemase-producing klebsiella pneumoniae was identified in the netherlands following foreign travel. they were a 55-year-old man who had undergone chemotherapy for lung cancer metastases, a 66-year-old woman and a 30-year-old man. the first patient was transferred from a greek hospital; his isolate belonged to an epidemic clone (multilocus sequence type 258) with a kpc-2 carbapenemase gene. the patient died from pneumonia. the other two patient ... | 2010 | 20858323 |
| in vivo antibacterial activity of nemonoxacin, a novel non-fluorinated quinolone. | to evaluate the in vivo antibacterial efficacy of nemonoxacin, a novel c8-methoxy non-fluorinated quinolone in murine systemic and local infection models. | 2010 | 20858687 |
| characterization of a defined cellulolytic and xylanolytic bacterial consortium for bioprocessing of cellulose and hemicelluloses. | diminishing fossil fuel reserve and increasing cost of fossil hydrocarbon products have rekindled worldwide effort on conversion of lignocellloloses (plant biomass) to renewable fuel. inedible plant materials such as grass, agricultural, and logging residues are abundant renewable natural resources that can be converted to biofuel. in an effort to mimic natural cellulolytic-xylanolytic microbial community in bioprocessing of lignocelluloses, we enriched cellulolytic-xylanolytic microorganisms, p ... | 2011 | 20859703 |
| clinical and molecular epidemiology of extended-spectrum β-lactamase-producing escherichia coli and klebsiella pneumoniae in a long-term study from japan. | the detection rates of extended-spectrum β-lactamase (esbl)-producing bacteria in japan are very low (∼5%) compared with those obtained worldwide. further, the current trend of these bacteria in japan is not known, and few studies with longitudinal observations have been reported. to obtain epidemiologic data on esbl-producing bacteria, their genotypic features, and their antibiotic resistance patterns in japan, we analyzed bacterial isolates from hospitalized patients at our institution over th ... | 2011 | 20859753 |
| systemic inflammatory response syndrome in home delivered neonates: a prospective observational study. | to determine the severity of systemic inflammatory response syndrome (sirs) at admission, bacteriological profile, antibiotic sensitivity of pathogens and factors associated with fatality in home delivered neonates with sepsis. | 2010 | 20859772 |
| evaluation of the diversilab system for detection of hospital outbreaks of infections by different bacterial species. | many bacterial typing methods are specific for one species only, time-consuming, or poorly reproducible. diversilab (dl; biomérieux) potentially overcomes these limitations. in this study, we evaluated the dl system for the identification of hospital outbreaks of a number bacterial species. appropriately typed clinical isolates were tested with dl. dl typing agreed with pulsed-field gel electrophoresis (pfge) for acinetobacter (n = 26) and stenotrophomonas maltophilia (n = 13) isolates. with two ... | 2010 | 20861340 |
| severe ventilator-associated pneumonia caused by methicillin-resistant staphylococcus aureus and other pathogens: report of a case successfully managed by serial surveillance cultures of endotracheal aspirates. | severe ventilator-associated pneumonia (vap) caused by methicillin-resistant staphylococcus aureus (mrsa) developed in a patient with acute respiratory distress syndrome. after tracheostomy, the patient was treated with antibiotic therapy guided by serial surveillance cultures of endotracheal aspirates. according to the result of the culture, the suitable antibiotic targeting the pathogen (e.g., mrsa, pseudomonas aeruginosa, klebsiella oxytoca) that had grown most predominantly in the culture wa ... | 2010 | 20862506 |
| stimulation of reductive glycerol metabolism by overexpression of an aldehyde dehydrogenase in a recombinant klebsiella pneumoniae strain defective in the oxidative pathway. | previously, we constructed a glycerol oxidative pathway-deficient mutant strain of klebsiella pneumoniae by inactivation of glycerol dehydrogenase (dhad) to eliminate by-product synthesis during production of 1,3-propanediol (1,3-pd) from glycerol. although by-product formation was successfully blocked in the resultant strain, the yield of 1,3-pd was not enhanced, probably because dhad disruption resulted in insufficient regeneration of the cofactor nadh essential for the activity of 1,3-pd oxid ... | 2010 | 20862513 |
| cockroaches (periplaneta americana and blattella germanica) as potential vectors of the pathogenic bacteria found in nosocomial infections. | although it has been difficult to prove the direct involvement of cockroaches (i.e. insects of the order blattaria) in the transmission of pathogenic agents to humans, such insects often carry microorganisms that are important in nosocomial infections, and their medical importance in the spread of bacteria cannot be ruled out. in houses and institutions with poor standards of hygiene, heavy infestations with cockroaches, such as the peridomestic american cockroach (periplaneta americana l.) and ... | 2010 | 20863441 |
| emergence of klebsiella pneumoniae of a novel sequence type (st383) producing vim-4, kpc-2 and cmy-4 β-lactamases. | | 2010 | 20863669 |
| cd44 deficiency is associated with increased bacterial clearance but enhanced lung inflammation during gram-negative pneumonia. | klebsiella pneumoniae is a frequently isolated causative pathogen in respiratory tract infections. cd44 is a transmembrane adhesion molecule that has been implicated in several immunological processes. to determine the role of cd44 during klebsiella pneumonia, we intranasally infected wild-type and cd44 knockout (ko) mice with 10(2) to 10(4) colony-forming units of k. pneumoniae or administered klebsiella lipopolysaccharide. during lethal infection, cd44 deficiency was associated with reduced ba ... | 2010 | 20864681 |
| update: detection of a verona integron-encoded metallo-beta-lactamase in klebsiella pneumoniae --- united states, 2010. | in july 2010, cdc was notified of a patient with a carbapenem-resistant klebsiella pneumoniae strain that produced a verona integron-encoded metallo-beta-lactamase (vim) carbapenemase not reported previously among enterobacteriaceae in the united states. the patient was a woman from the united states who became ill with diarrhea during a mediterranean cruise and was hospitalized in greece, where she received a diagnosis of sepsis and clostridium difficile infection. after 12 days in two hospital ... | 2010 | 20864922 |
| klebsiella pneumoniae-triggered dc recruit human nk cells in a ccr5-dependent manner leading to increased ccl19-responsiveness and activation of nk cells. | besides their role in destruction of altered self-cells, nk cells have been shown to potentiate t-cell responses by interacting with dc. to take advantage of nk-dc crosstalk in therapeutic dc-based vaccination for infectious diseases and cancer, it is essential to understand the biology of this crosstalk. we aimed to elucidate the in vitro mechanisms responsible for nk-cell recruitment and activation by dc during infection. to mimic bacterial infection, dc were exposed to a membrane fraction of ... | 2010 | 20865789 |
| impact of microbial tolerance in persistent secondary klebsiella pneumoniae peritonitis. | microbial tolerance represents a diminished pro-inflammatory response following repeated stimulation by a host of pathogen-associated molecular patterns (pamps) of varying origins. toll-like receptors (tlrs) have been centrally implicated in the development of tolerance. the purpose of this study was to investigate the impact of tolerance in a previously described murine model. c57bl/6 mice were pretreated intraperitoneally with phosphate buffered saline (pbs), heat-killed klebsiella 2×10(8) cfu ... | 2011 | 20869262 |
| evaluation and validation of a novel arxula adeninivorans estrogen screen (naes) assay and its application in analysis of wastewater, seawater, brackish water and urine. | a novel arxula adeninivorans yeast estrogen screen (naes) assay has been developed for detection of estrogenic activity in various liquid samples such as wastewater, seawater, brackish water and swine urine. two bio-components were engineered to co-express the human estrogen receptor α (herα) and an inducible reporter gene; either the non-conventional phytase gene (phyk, derived from klebsiella sp. asr1) or the non-conventional tannase gene (atan1, derived from arxula). both reporters were put u ... | 2010 | 20869755 |
| emergence of carbapenem resistance due to porin loss in an extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae strain during meropenem therapy. | | 2010 | 20869852 |
| multiclonal emergence of carbapenem-resistant klebsiella pneumoniae in tuscany, italy. | | 2010 | 20869854 |
| light insensitive silver(i) cyanoximates as antimicrobial agents for indwelling medical devices. | ten silver(i) cyanoximates of agl composition (l = nc-c(no)-r, where r is electron withdrawing groups: -cn, -c(o)nr(2), -c(o)r' (alkyl), -c(o)oet, 2-heteroaryl fragments such as 2-pyridyl, 2-benzimidazolyl, 2-benzoxazolyl, 2-benzthiazolyl) were synthesized and characterized using spectroscopic methods and x-ray analysis. crystal structures of four complexes were determined and revealed the formation of two-dimensional (2d) coordination polymers of different complexity in which anions exhibit bri ... | 2010 | 20873734 |
| isolation and characterization of bacteria from the gut of bombyx mori that degrade cellulose, xylan, pectin and starch and their impact on digestion. | bombyx mori l. (lepidoptera: bombycidae) have been domesticated and widely used for silk production. it feeds on mulberry leaves. mulberry leaves are mainly composed of pectin, xylan, cellulose and starch. some of the digestive enzymes that degrade these carbohydrates might be produced by gut bacteria. eleven isolates were obtained from the digestive tract of b. mori, including the gram positive bacillus circulans and gram negative proteus vulgaris, klebsiella pneumoniae, escherichia coli, citro ... | 2010 | 20874394 |
| recurrent healthcare-associated community-onset infections due to klebsiella pneumoniae producing vim-1 metallo-beta-lactamase. | to investigate the extrahospital dissemination of carbapenem-resistant klebsiella pneumoniae isolates and the mechanisms of acquired resistance. | 2010 | 20876622 |
| assessment of euphorbia hirta l. leaf, flower, stem and root extracts for their antibacterial and antifungal activity and brine shrimp lethality. | the antimicrobial activities of the methanolic extracts of euphorbia hirta l leaves, flowers, stems and roots were evaluated against some medically important bacteria and yeast using the agar disc diffusion method. four gram positive (staphylococcus aureus, micrococcus sp., bacillus subtilis and bacillus thuringensis), four gram negative (escherichia coli, klebsiella pneumonia, salmonella typhi and p. mirabilis) and one yeast (candida albicans) species were screened. inhibition zones ranged betw ... | 2010 | 20877206 |
| bacteraemic community-acquired pneumonia due to gram-negative bacteria: incidence, clinical presentation and factors associated with severity during hospital stay. | many studies have evaluated the clinical characteristics of gram-negative bacteria (gnb) pneumonia. however, in most cases the bacteriological diagnosis is based on unreliable respiratory samples, and research rarely focuses on only bacteraemic patients. the aim of this study was to describe the incidence, clinical characteristics, outcomes, and factors associated with severity during the hospital stay of patients diagnosed with bacteraemic community-acquired pneumonia (cap) due to gnb. | 2010 | 20878457 |
| increase of ceftazidime- and fluoroquinolone-resistant klebsiella pneumoniae and imipenem-resistant acinetobacter spp. in korea: analysis of konsar study data from 2005 and 2007. | antimicrobial resistance monitoring could be a useful source of information for treating and controlling nosocomial infections. we analyzed antimicrobial resistance data generated by korean hospitals and by a commercial laboratory in 2005 and 2007. | 2010 | 20879058 |
| evaluation of updated interpretative criteria for categorizing klebsiella pneumoniae with reduced carbapenem susceptibility. | we studied the accuracy of various susceptibility testing methods, including the 2009, 2010, and updated 2010 clsi recommendations, to identify klebsiella pneumoniae isolates with reduced susceptibility to carbapenems associated with different mechanisms of resistance. forty-three wild-type (wt) strains, 42 extended-spectrum β-lactamase (esbl) producers, 18 esbl producers with outer membrane porin protein loss (esbl/omp strains), and 42 blakpc-possessing k. pneumoniae (kpc-kp) isolates were eval ... | 2010 | 20881179 |
| etiology and antibiotic susceptibility patterns of community- and hospital-acquired urinary tract infections in a general hospital in kuwait. | our purpose was to determine the bacterial profile and prevalence of antibiotic resistance patterns of uropathogens, as well as evaluate the problem with extended-spectrum β-lactamase (esbl) producing isolates, causing urinary tract infections (utis) in al-amiri hospital, kuwait, over a 3-year period. | 2010 | 20881410 |
| alimentary tract bacteria isolated and identified with api-20e and molecular cloning techniques from australian tropical fruit flies, bactrocera cacuminata and b. tryoni. | bacteria were isolated from the crop and midgut of field collected bactrocera cacuminata (hering) and bactrocera tryoni (froggatt) (diptera: tephritidae). two methods were used, firstly isolation onto two types of bacteriological culture media (pyea and tsa) and identification using the api-20e diagnostic kit, and secondly, analysis of samples using the 16s rrna gene molecular diagnostic method. using the api-20e method, 10 genera and 17 species of bacteria in the family enterobacteriaceae were ... | 2010 | 20883132 |
| tattoo related pyoderma/ectyma gangrenous as presenting feature of relapsed acute myeloid leukaemia: an exceptionally rare observation. | | 2010 | 20883993 |
| modern treatment of infected total knee arthroplasty with a 2-stage reimplantation protocol. | the purpose of this study was to determine if 2-stage reimplantation for the treatment of infected total knee arthroplasty (tka) is still effective for treating contemporary pathogens, many of which are multidrug resistant (mdr). the medical records of all cases of infected tkas from april 1998 to march 2006 were retrospectively reviewed for data on infecting organism and success of treatment. of 72 patients (75 knees), with a minimum of 2 years of follow-up, who completed the protocol, the infe ... | 2010 | 20888545 |
| [etiology and sensitivity of uropathogens identified in uncomplicated lower urinary tract infections in women (aresc study): implications on empiric therapy]. | to determine the etiology and susceptibility of uropathogens identified in women with uncomplicated lower urinary tract infections (uti). | 2011 | 20889171 |
| acute diffuse phlegmonous esophagogastritis: a case report. | acute phlegmonous infection of the gastrointestinal tract is characterized by purulent inflammation of the submucosa and muscular layer with sparing of the mucosa. the authors report a rare case of acute diffuse phlegmonous esophagogastritis, which was well diagnosed based on the typical chest computed tomographic (ct) findings and was successfully treated. a 48-yr-old man presented with left chest pain and dyspnea for three days. chest radiograph on admission showed mediastinal widening and bil ... | 2010 | 20890440 |
| identification and characterization of the propanediol utilization protein pdup of lactobacillus reuteri for 3-hydroxypropionic acid production from glycerol. | although the de novo biosynthetic mechanism of 3-hydroxypropionic acid (3-hp) in glycerol-fermenting microorganisms is still unclear, the propanediol utilization protein (pdup) of lactobacillus species has been suggested to be a key enzyme in this regard. to verify this hypothesis, a pdup gene from lactobacillus reuteri was cloned and expressed, and the encoded protein was characterized. recombinant l. reuteri pdup exhibited broad substrate specificity including 3-hydroxypropionaldehyde and util ... | 2010 | 20890600 |
| acute lung inflammation in klebsiella pneumoniae b5055-induced pneumonia and sepsis in balb/c mice: a comparative study. | lungs play an important role in the body's defense against a variety of pathogens, but this network of immune system-mediated defense can be deregulated during acute pulmonary infections. the present study compares acute lung inflammation occurring during klebsiella pneumoniae b5055-induced pneumonia and sepsis in balb/c mice. pneumonia was induced by intranasal instillation of bacteria (10(4) cfu), while sepsis was developed by placing the fibrin-thrombin clot containing known amount of bacteri ... | 2010 | 20890649 |
| the impact of fluoroquinolones use on antibiotic resistance in an intensive care burn department. | fluoroquinolones (fq) use has been identified as a risk factor for colonization and infection to methicillin resistant staphylococcus aureus(mrsa), pseudomonas aeruginosae multiresistant(pmr), acinetobacter multiresistant (amr) and multidrug resistant bacteria(mdrb). | 2010 | 20890813 |
| [antibiotic prophylaxis in prostate biopsy]. | prostate biopsy is nowadays one of the most frequent diagnostic procedures in urology. the incidence of bacteraemia, bacteriuria and infective complications is higher after the transrectal procedure than after the transperineal one. a survey demonstrated that 98% of the urologists in usa use antibiotics to prevent infective complications. the transrectal prostate biopsy is the only diagnostic intervention procedure in urology for which an antibiotic prophylaxis is recommended, also for low-risk ... | 2010 | 20890852 |
| [bacteria isolated from surgical infections and their susceptibilities to antimicrobial agents --special references to bacteria isolated between april 2008 and march 2009]. | bacteria isolated from infections in abdominal surgery during the period from april 2008 to march 2009 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 712 strains including 18 strains of candida spp. were isolated from 173 (80.5%) of 215 patients with surgical infections. three hundred and sixty-six strains were isolated from primary infections, and 346 strains were isolated from postoperative infections. from primary infections, anaero ... | 2010 | 20919496 |
| postoperative bacteremia in periodontal flap surgery, with and without prophylactic antibiotic administration: a comparative study. | many a times in clinical periodontology, the decision whether to prescribe prophylactic antibiotics or not, is perplexing.the present study was conducted to compare the bacteremias induced after periodontal flap surgeries with and without prophylactic antibiotics. | 2010 | 20922074 |
| antimicrobial activities of single aroma compounds. | commercially available aroma samples were evaluated for their olfactory quality by professional perfumers and tested for their antimicrobial activity. agar diffusion and agar-dilution were used as test methods and a set of two gram-positive (staphylococcus aureus and enterococcus faecalis) and four gram-negative bacterial strains (escherichia coli, pseudomonas aeruginosa, proteus vulgaris g, klebsiella pneumoniae and salmonella abony) and a yeast, candida albicans, were the test microorganisms. ... | 2010 | 20922992 |
| antimicrobial properties of volatile phenylpropanes. | the examination of antimicrobial structure-activity relationships of 93 volatile phenylpropanes (vps) and 21 related aromatic compounds revealed a dependence of antimicrobial activity from the kind and number of substituents on the aromatic ring, their substitution pattern and microbial characteristics, such as gram coloring and strain specific factors. eugenol isomers were predominantly inhibitory in a concentration range from 25 to 2000 mg/l against all microorganisms tested, which were three ... | 2010 | 20922996 |
| coupling between the bluf and eal domains in the blue light-regulated phosphodiesterase blrp1. | the first biochemical and structural characterization of the full-length active photoreceptor blrp1 from klebsiella pneumoniae was recently reported by barends et al. [nature 459:1015-1018, (2009)]. the light-regulated catalytic function of its c-terminal c-di-guanosine monophosphate phosphodiesterase, the eal (glu-ala-leu) domain, is activated by the n-terminal sensor of blue light using the flavin adenine dinucleotide (bluf) domain. we performed molecular dynamics simulations on the dimeric bl ... | 2010 | 20924626 |
| extended-spectrum β-lactamases in gram negative bacteria. | extended-spectrum β-lactamases (esbls) are a group of plasmid-mediated, diverse, complex and rapidly evolving enzymes that are posing a major therapeutic challenge today in the treatment of hospitalized and community-based patients. infections due to esbl producers range from uncomplicated urinary tract infections to life-threatening sepsis. derived from the older tem is derived from temoniera, a patient from whom the strain was first isolated in greece. β-lactamases, these enzymes share the abi ... | 2010 | 20927289 |
| progress and challenges in implementing the research on eskape pathogens. | the eskape pathogens (enterococcus faecium, staphylococcus aureus, klebsiella pneumoniae, acinetobacter baumannii, pseudomonas aeruginosa, and enterobacter species) are responsible for a substantial percentage of nosocomial infections in the modern hospital and represent the vast majority of isolates whose resistance to antimicrobial agents presents serious therapeutic dilemmas for physicians. over the years, improved molecular biology techniques have led to detailed information about individual ... | 2010 | 20929376 |
| tigecycline activity: low resistance rates but problematic disc breakpoints revealed by a multicentre sentinel survey in the uk. | a multicentre surveillance of tigecycline activity against relevant pathogens in the uk. | 2010 | 20930043 |
| molecular characterization and antimicrobial susceptibility of extended-spectrum {beta}-lactamase-producing enterobacteriaceae isolates at a tertiary-care centre in monterrey, mexico. | our objective was to analyse phenotypic and genetic data of extended-spectrum β-lactamase (esbl)-producing klebsiella pneumoniae, enterobacter cloacae, escherichia coli and serratia marcescens that cause infections in our hospital. over a 3 year period, 342 randomly selected clinical enterobacteriaceae isolates were tested for esbl production and evaluated for the presence of the β-lactamase genes bla(shv), bla(tem,) bla(ctx-m) and bla(tla-1). the antibiotic susceptibilities of these isolates we ... | 2010 | 20930052 |
| carbapenem-resistant enterobacteriaceae and acinetobacter baumannii: assessing their impact on organ transplantation. | purpose of review: this review highlights the impact of carbapenem-resistant enterobacteriaceae and carbapenem-resistant acinetobacter baumannii on patients who have undergone organ transplantation and explores both available and potential agents to treat infections caused by these multidrug-resistant (mdr) pathogens. recent findings: few antimicrobials exist to treat carbapenem-resistant gram-negative infections, and resistance to salvage therapies is escalating. organ transplantation appears t ... | 2010 | 20930636 |
| protective effects of bioactive phytochemicals from mentha piperita with multiple health potentials. | mentha piperita essential oil was bactericidal in order of e. coli> s. aureus > pseudomonas aeruginosa > s. faecalis > klebsiella pneumoniae. the oil with total phenolics of 89.43 ± 0.58 µg gae/mg had 63.82 ± 0.05% dpph inhibition activity with an ic (50) = 3.9 µg/ml. lipid peroxidation inhibition was comparable to bht and bha. a 127% hike was noted in serum ferric-reducing antioxidant power. there was 38.3% decrease in wbcs count, while platelet count showed increased levels of 214.12%. signifi ... | 2010 | 20931070 |
| essential oil constituents of illicium griffithii and its antimicrobial activity. | the essential oil of the fruit of illicium griffithii hook f. et thoms. was extracted using clevenger's apparatus. forty-one compounds were characterized by gas chromatography-mass spectroscopy (gc-ms). 4-methyl-6-(2-propenyl)-1,3-benzodioxole was characterized as the major constituent, followed by linalool amongst the volatile constituents. the essential oil was found to be effective against aspergillus niger, penicillium spp. and saccharomyces cerevisiae and possessed considerable activity aga ... | 2010 | 20931081 |
| urinary tract infections in children with primary nephrotic syndrome and acute glomerulonephritis. | the occurrence of urinary tract infection (uti) in children with nephrotic syndrome (ns) has been widely reported by various workers, but not much has been documented about its occurrence among children with acute glomerulonephritis (agn). hence, the level of susceptibility to uti by both diseases has not been compared. | 2010 | 20931510 |
| distribution of the putative type vi secretion system core genes in klebsiella spp. | the type vi secretion system (t6ss) is a recently characterized secretion system which appears to be involved in bacterial pathogenesis as a potential nano-syringe for the translocation of effector proteins into the eukaryotic host cell cytoplasm. until now no evidence was provided for the presence of t6ss in the genomes of the sequenced representatives of klebsiella spp., including the human opportunistic pathogen klebsiella pneumoniae. however, in a previous study by lawlor et al. (2005), were ... | 2010 | 20932940 |
| efficient synthesis, spectral analysis and antimicrobial studies of nitrogen and sulfur containing spiro heterocycles from 2,4-diaryl-3-azabicyclo[3.3.1]nonan-9-ones. | acetyl and propionyl group substituted thiadiazole derivatives (4a-4h, 5a-5h, 6a, 6b, 7a and 7b) have been synthesized by the cyclization of 2,4-diaryl-3-azabicyclo[3.3.1]nonan-9-one thiosemicarbazones (2a-2h, 3a and 3b) with acetic anhydride/propionic anhydride and were characterized by elemental analysis, ir, (1)h nmr and (13)c nmr spectral analysis. single crystal x-ray diffraction has also been recorded for compounds 4c and 5a. from the nmr and single crystal x-ray diffraction analysis, comp ... | 2010 | 20933408 |
| replicon sequence typing of incf plasmids carrying virulence and resistance determinants. | incf plasmids are frequently encountered in clinical enterobacterial strains associated with the dissemination of relevant antimicrobial resistance and virulence genes. these plasmids are usually heterogeneous in size and carry multiple replicons, and technical difficulties can impair the comparison and detection of related plasmids by restriction fragment length polymorphism analysis. we devised a rapid sequence-based typing scheme to categorize the members of this plasmid family into homogeneo ... | 2010 | 20935300 |
| swab and aspiration specimen collection methods and antibiogram in chronic suppurative otitis media at jos university teaching hospital: which is superior? | chronic suppurative otitis media is a very common otologic problem in our environment. appropriate methods for obtaining sample specimens for specific bacteria isolation has generated a lot of controversy. the simplest method available in our environment is the traditional swab method which, however, has been condemned on the basis of introducing contaminants. the objectives of this study were to compare the bacterial yield and the antibiogram of two specimen collection methods: the traditional ... | 2010 | 20935423 |
| nosocomial infections and multidrug-resistant bacterial organisms in the pediatric intensive care unit. | nosocomial infections in pediatric intensive care units (picus) caused by multidrug-resistant bacterial organisms are increasing. this review attempts to report on significant findings in the current literature related to nosocomial infections in picu settings with an international perspective. the types of nosocomial infections are addressed, including catheter-related bloodstream infections, ventilator-associated pneumonia, urinary tract infections, gastrointestinal infections and post-surgica ... | 2010 | 20936380 |
| cxcl1 regulates pulmonary host defense to klebsiella infection via cxcl2, cxcl5, nf-kappab, and mapks. | pulmonary bacterial infections are a leading cause of death. since the introduction of antibiotics, multidrug-resistant klebsiella pneumoniae became an escalating threat. therefore, development of methods to augment antibacterial defense is warranted. neutrophil recruitment is critical to clear bacteria, and neutrophil migration in the lung requires the production of elr(+) cxc chemokines. although lung-specific cxcl1/keratinocyte cell-derived chemokine (kc) transgene expression causes neutrophi ... | 2010 | 20937845 |
| unusual pneumoperitoneum secondary to ruptured liver abscess. | | 2010 | 20938254 |
| an effective and simplified fed-batch strategy for improved 2,3-butanediol production by klebsiella oxytoca. | substrate concentration in 2,3-butanediol (2,3-bd) fermentation could not be controlled well in traditional feeding strategies, such as constant, impulse, and exponential feeding strategies. in the present study, fermentative 2,3-bd production by klebsiella oxytoca was investigated under different batch and fed-batch strategies. the glucose-feedback fed-batch strategy was proved to be not effective for economical 2,3-bd production for the inability of timely feeding, leading that the bacteria re ... | 2010 | 20938754 |