| control of coliform bacteria detected from diarrhea associated patients by extracts of moringa oleifera. | the aims of this study were to determine the total population of coliform bacteria in the samples collected from diarrhea associated patients from the local area of bangladesh and to examine the antibacterial efficacy of leaf extracts of moringa oleifera (moringaceae) against the isolated coliform bacteria. the coliform bacteria detected in these samples by some microbial-biochemical tests such as escherichia coli, shigella dysenteriae, salmonella sp., enterobacter sp., klebsiella pneumoniae and ... | 2010 | 20677603 |
| antimycobacterial activity of udp-galactopyranose mutase inhibitors. | the galactofuran region of the mycobacterial cell wall consists of alternating 5- and 6-linked beta-d-galactofuranose (beta-d-galf) residues, essential for viability. udp-galactofuranose (udp-galf), the donor for galf, is synthesised from udp-galactopyranose (udp-galp) by the enzyme udp-galactopyranose mutase (ugm), which is not found in humans, rendering it a therapeutic target. the in vitro properties, i.e. enzymatic activity, antimycobacterial activity, cellular toxicity, activity in mycobact ... | 2010 | 20678902 |
| membrane efflux and influx modulate both multidrug resistance and virulence of klebsiella pneumoniae in a caenorhabditis elegans model. | cross-resistance to cefoxitin (fox), chloramphenicol (cmp), and quinolones (nalidixic acid [nal]) related to a putative efflux system overexpression has recently been reported for klebsiella pneumoniae. the potential impact of this multidrug resistance (mdr) on the virulence of k. pneumoniae was evaluated in the caenorhabditis elegans model. for 2 of the 3 mdr clinical isolates studied, a significant increase in acrb transcription was found in comparison with their antibiotic-susceptible reverta ... | 2010 | 20679507 |
| a report from the 20th european congress of clinical microbiology and infectious diseases (april 10-13, 2010 - vienna, austria). | effective antimicrobials currently in use in europe and throughout the world are fast losing ground as many pathogens acquire resistance to newly introduced drugs. multidrug and panresistance have now been identified in many pathogens, as iteratively discussed throughout this year's meeting of the european congress of clinical microbiology and infectious diseases (eccmid). as extensively discussed in an oral session entitled worldwide dissemination of resistances by 10 specialists from across eu ... | 2010 | 20683506 |
| in vitro evaluation of antibiotic synergy for polymyxin b-resistant carbapenemase-producing klebsiella pneumoniae. | since carbapenemase-producing klebsiella pneumoniae strains were first reported in north carolina, these highly resistant organisms have been isolated with increasing frequency, especially in the new york city area. polymyxin b is one of the few antimicrobials that retain reliable activity against these organisms. however, polymyxin b mics are elevated against k. pneumoniae isolates with increasing frequency, leaving clinicians with few therapeutic options. we investigated several antimicrobial ... | 2010 | 20686085 |
| synthesis and antibacterial activity of some novel 6-(1h-benz[d]imidazol-2-yl)-8-(5-nitro-2-furyl)-3-(4-pyridyl)-7,8-dihydro[1,2,4]triazolo[3,4-b][1,3,4]thiadiazepines. | a new series of novel 6-(1h-benz[d]imidazol-2-yl)-8-(5-nitro-2-furyl)-3-(4-pyridyl)-7,8-dihydro[1,2,4]triazolo[3,4-b][1,3,4]thiadiazepines 8a-d has been synthesized. these compounds were evaluated for their efficiency as antibacterial agents against two gram-positive and gram-negative strains of bacteria along with antifungal activity against two fungal organisms. the antibacterial and antifungal activities of the present compounds were not comparable with those of the standard drugs employed. b ... | 2010 | 20686263 |
| overview perspective of bacterial resistance. | the rapidly escalating prevalence of antimicrobial resistance is a global concern. this reduced susceptibility to currently available antimicrobial agents coupled with the progressive shortage of newly approved compounds is a worrisome situation. major problems are encountered for a growing number of gram-positive (i.e., staphylococcus aureus, streptococcus pneumoniae, enterococcus spp.) and gram-negative pathogens (i.e., pseudomonas aeruginosa, acinetobacter baumannii, klebsiella pneumoniae). w ... | 2010 | 20687782 |
| [healthcare associated infection: emerging problem in a neurology department]. | healthcare-associated infection (hai) is, nowadays, a serious problem, contributing a lot to the burden of the hospital stays. its impact is really huge and the study of these matters is taking a growing interest. | 2010 | 20687989 |
| [evaluation of the antimicrobial susceptibility profile of microorganisms isolated from urine in the region of vale do sousa and tâmega]. | the urinary tract is one of the more common sites of bacterial infections, especially in women. urinary infection can be defined as an infection of urinary tract structures which occurs, generally, as a consequence of the presence or colonization by urine bacteria. the aim of this study was to determine the etiology of urinary tract infections and their susceptibility to antimicrobial agents in the region of vale do sousa and tâmega. from february 2008 to january 2009, 18653 urine cultures were ... | 2010 | 20687992 |
| influence of third-generation cephalosporin resistance on adult in-hospital mortality from post-neurosurgical bacterial meningitis. | to investigate the clinical features, etiology and predictors of in-hospital mortality in adults with post-neurosurgical bacterial meningitis. | 2010 | 20688290 |
| genotypic analysis of plasmid-mediated beta-lactamases amongst enterobacteriaceae other than escherichia spp. and klebsiella spp. that are non-susceptible to a broad-spectrum cephalosporin. | enterobacteriaceae other than escherichia spp. and klebsiella spp. are recognisable for their increasing resistance. plasmid-mediated beta-lactamases, including extended-spectrum beta-lactamases (esbls), plasmid-mediated ampc beta-lactamases (pampcs) and the newly emerged kpc, play an important role in beta-lactam resistance. in this study, we investigated the genetic characteristics of plasmid-mediated beta-lactamases amongst non-escherichia, non-klebsiella enterobacteriaceae that were non-susc ... | 2010 | 20688485 |
| prevalence of hypermucoid klebsiella pneumoniae among wild-caught and captive vervet monkeys (chlorocebus aethiops sabaeus) on the island of st. kitts. | invasive, hypermucoid klebsiella pneumoniae causes severe abscess formation in humans and in certain species of nonhuman primates. we conducted a survey of captive and wild-caught african green monkeys, or vervets (chlorocebus aethiops sabaeus), on the caribbean island of st. kitts to assess their carriage rate of klebsiella spp. forty percent of rectal swabs from captive monkeys were positive for k. pneumoniae, and 20% of wild-caught animals were positive. two wild-caught monkeys (4%) were posi ... | 2010 | 20688708 |
| successful treatment of pan-resistant klebsiella pneumoniae pneumonia and bacteraemia with a combination of high-dose tigecycline and colistin. | the spread of antimicrobial resistance among members of the enterobacteriaceae is a significant clinical threat. we report the treatment of pan-resistant klebsiella pneumoniae bacteraemia with combination tigecycline and colistin in a 49-year-old male and review available therapeutic options. despite a poor prognosis, the patient recovered, but remains colonized with the pan-resistant isolate. | 2010 | 20688947 |
| genetic and functional diversity among the antagonistic potential fluorescent pseudomonads isolated from tea rhizosphere. | twenty-five fluorescent pseudomonads from rhizospheric soil of six tea gardens in four district of upper assam, india were isolated and screened for antagonistic activity against fungal pathogens such as fusarium oxysporum f. sp. raphani (for), fusarium oxysporum f. sp. ciceri (foc), fusarium semitectum (fs), and rhizoctonia solani (rs); and bacterial pathogens-staphylococcus aureus (sa), escherichia coli (ec), and klebsiella pneumoniae (kp). most of the isolates exhibited strong antagonistic ac ... | 2011 | 20689953 |
| serum proteomic analysis from bacteremic and leucopenic rabbits. | background: bacteremia causes mild to life-threatening illnesses as a consequence of inflammatory pathway activation, and leucopenia can increase mortality rates and treatment costs. the identification of novel biomarkers for leucopenia in the context of bacteremia would facilitate early detection and provide insights into the identification of novel therapeutic targets. methods: rabbits were infected with klebsiella pneumoniae to induce bacteremia with leucopenia, and sera from infected and non ... | 2010 | 20691998 |
| outbreak of severe sepsis due to contaminated propofol: lessons to learn. | nosocomial infections are a frequent concern in healthcare. despite the available knowledge on nosocomial infections and preventive measures, outbreaks of infections continue to occur. an outbreak of severe sepsis in patients who underwent minor procedures in an operating theatre during two consecutive days is described and analysed in this study. we performed a retrospective cohort study using epidemiological data in order to investigate the source of infection together with microbiological and ... | 2010 | 20692067 |
| genetic analysis of the nitrogen assimilation control protein from klebsiella pneumoniae. | the nitrogen assimilation control protein (nac) from klebsiella pneumoniae is a typical lysr-type transcriptional regulator (lttr) in many ways. however, the lack of a physiologically relevant coeffector for nac and the fact that nac can carry out many of its functions as a dimer make nac unusual among the lttrs. in the absence of a crystal structure for nac, we analyzed the effects of amino acid substitutions with a variety of phenotypes in an attempt to identify functionally important features ... | 2010 | 20693327 |
| prevalence of multidrug-resistant bacteria at a tertiary-care teaching hospital in mexico: special focus on acinetobacter baumannii. | our aim was to determine the prevalence of multidrug resistance of acinetobacter baumannii and other pathogens at a tertiary-care teaching hospital in mexico over a 3-year period. clinical isolates of a. baumannii (n = 550), pseudomonas aeruginosa (n = 250), some enterobacteriaceae species (n = 500) and staphylococcus aureus (n = 250) collected over a 3-year period were included. susceptibility tests were performed by the broth microdilution method. 74% of a. baumannii, 40% of escherichia coli, ... | 2010 | 20693798 |
| epidemiologic trends, occurrence of extended-spectrum beta-lactamase production, and performance of ertapenem and comparators in patients with intra-abdominal infections: analysis of global trend data from 2002-2007 from the smart study. | the study for monitoring antimicrobial resistance trends (smart) is an ongoing surveillance study to monitor worldwide resistance trends among aerobic and facultative anaerobic gram-negative bacilli isolated from intra-abdominal infections (iais). results from smart in 2002 through 2005 have been reported; this report summarizes aggregated data from smart 2002-2007 to identify epidemiologic trends over time. | 2010 | 20695829 |
| complete nucleotide sequence of kpc-3-encoding plasmid pkpqil in the epidemic klebsiella pneumoniae sequence type 258. | we have determined the entire dna sequence of plasmid pkpqil, the bla(kpc-3)-carrying plasmid harbored by the carbapenem-resistant klebsiella pneumoniae clone sequence type 258 (st 258) in israel. pkpqil is a 113,637-bp, self-transmissible plasmid that belongs to the incompatibility group incfii. it consists of a large backbone of a pkpn4-like plasmid and carries the bla(kpc-3)-containing tn4401a transposon of a pnyc-like plasmid. | 2010 | 20696875 |
| evolution of tigecycline resistance in klebsiella pneumoniae in a single patient. | carbapenemase-producing klebsiella pneumoniae infections carry serious clinical and infection-control implications. isolates possessing such hydrolyzing enzymes have been described in the united states and around the world. besides being resistant to carbapenems, they usually confer resistance to fluoroquinolones, piperacillin-tazobactam, and extended-spectrum cephalosporins. tigecycline demonstrates in vitro activity against these organisms, but reported resistance raises concern about tigecycl ... | 2010 | 20697161 |
| prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in saudi arabia. | patients in the icu have encountered an increasing emergence and spread of antibiotic-resistant pathogens. we examined patterns of antimicrobial susceptibility in gram-negative isolates to commonly used drugs in an adult icu at a tertiary care hospital in riyadh, saudi arabia. | 2010 | 20697174 |
| serum tumor necrosis factor alpha and c-reactive protein in pediatric patients with sepsis and its correlation with microbiologic findings. | to study the association of tumor necrosis factor-a (tnf-a) and c--reactive protein (crp) with microbiologically documented cases of sepsis versus clinically documented cases of sepsis. | 2010 | 20699510 |
| [characteristic features of urinary tract infection in malnourished children]. | the aim of this study is to prove a relationship between urinary tract infection and malnutrition in children 0-3 years old, hospitalized in the ivi pediatric clinic, hospital st. mary iaşi, between january 2000 and december 2004. | 2010 | 20700977 |
| [chromogenic medium for detection of enterobacteria producing beta-lactamases in north-eastern romania]. | we evaluated the performances and analyzed practicability of an esbl-detecting chromogenic agar medium (chromid esbl, biomerieux) for the screening of esbl production. | 2010 | 20700996 |
| prevalence of extended-spectrum beta-lactamase-producing enterobacteriaceae in bahrain. | to determine the occurrence of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in bahrain. | 2009 | 20701872 |
| the pan genera detection immunoassay: a novel point-of-issue method for detection of bacterial contamination in platelet concentrates. | bacterial contamination of platelet concentrates (pcs) still represents an ongoing risk in transfusion-transmitted sepsis. recently the pan genera detection (pgd) system was developed and fda licensed for screening of bacterial contamination of pcs directly prior to transfusion. the test principle is based on the immunological detection of lipopolysaccharide (for gram-negative bacteria) or lipoteichoic acid (for gram-positive bacteria). in the present study we analyzed the applicability of this ... | 2010 | 20702673 |
| [umbilical venous catheterization related infection in the neonatal intensive care unit]. | to study the incidence and pathogens of umbilical venous catheterization (uvc) related infection in the neonatal intensive care unit (nicu). | 2010 | 20704793 |
| hospital outbreak caused by klebsiella pneumoniae producing kpc-2 beta-lactamase resistant to colistin. | we describe a hospital outbreak caused by colistin-resistant klebsiella pneumoniae producing kpc-2 beta-lactamase in two distinct medical centres. seven clinical isolates of k. pneumoniae exhibiting resistance to carbapenems were collected from patients with hospital-acquired infection. all isolates were phenotypically positive for carbapenemase activity but negative for metallo-beta-lactamase production. pcr analysis using specific primers for bla(kpc), bla(shv), bla(tem) and bla(ctx-m) demonst ... | 2010 | 20705205 |
| stereoselective synthesis and biological evaluation of (r)-rugulactone, (6r)-((4r)-hydroxy-6-phenyl-hex-2-enyl)-5,6-dihydro-pyran-2-one and its 4s epimer. | a simple and highly efficient synthetic route has been developed for synthesis of (r)-rugulactone (1a), (6r)-((4r)-hydroxy-6-phenyl-hex-2-enyl)-5,6-dihydro-pyran-2-one (1b) and its 4s epimer 1c by employing proline-catalyzed alpha-aminooxylation, sharpless epoxidation, mitsunobu reaction as chirality introuducing steps. the antibacterial and antifungal activity of the compounds 1a, 1b and 1c were evaluated. 1a and 1b showed better antibacterial activity against pseudomonas aeroginosa (mic=12.5 m ... | 2010 | 20705370 |
| the latest threat in the war on antimicrobial resistance. | | 2010 | 20705515 |
| emergence of a new antibiotic resistance mechanism in india, pakistan, and the uk: a molecular, biological, and epidemiological study. | gram-negative enterobacteriaceae with resistance to carbapenem conferred by new delhi metallo-beta-lactamase 1 (ndm-1) are potentially a major global health problem. we investigated the prevalence of ndm-1, in multidrug-resistant enterobacteriaceae in india, pakistan, and the uk. | 2010 | 20705517 |
| strange formation of n-hydroxy-3,3-dimethyl-2,6-diarylpiperidin-4-one and its oxime derivative--synthesis, stereochemistry, antibacterial and antifungal activity. | objectives and methods: a collection of novel n-hydroxy-3,3-dimethyl-2,6-diarylpiperidin-4-one oximes 28-32 are synthesized, characterized by melting point, elemental analysis, ms, ft-ir, one-dimensional nmr (1h & 13c) and two dimensional noesy spectroscopic data and evaluate for their in vitro antibacterial and antifungal activities. results and conclusion: compound 32, exerted a wide range of antibacterial activities against the entire tested gram-positive and gram-negative bacterial strains e ... | 2010 | 20707249 |
| screening of antibacterial activity of mucus extract of snakehead fish, channa striatus (bloch). | the objective of this study is to gain a better understanding of the antimicrobial properties of the mucus extract of snakehead fish, channa striatus against selected human and fish pathogenic microbes. | 2010 | 20707287 |
| in vitro antibacterial evaluation of anabaena sp. against several clinically significant microflora and hptlc analysis of its active crude extracts. | the present study was conducted to evaluate the possible antibacterial activity of anabaena extracts. anabaena was isolated from a natural source and cultured in vitro. after suitable growth, cyanobacterial culture was harvested using different solvents. extracts, thus prepared, were evaluated for their antibacterial potential by agar-well diffusion assay against bacterial species of clinical significance. mic values were determined further to check the concentration ranges for significant inhib ... | 2010 | 20711376 |
| structural changes induced by a lytic bacteriophage make ciprofloxacin effective against older biofilm of klebsiella pneumoniae. | bacteria have evolved multiple mechanisms, such as biofilm formation, to thwart antibiotic action. yet antibiotics remain the drug of choice against clinical infections. it has been documented that young biofilm of klebsiella pneumoniae could be eradicated significantly by ciprofloxacin treatment alone. since age of biofilm is a decisive factor in determining the outcome of antibiotic treatment, in the present study biofilm of k. pneumoniae, grown for extended periods was treated with ciprofloxa ... | 2010 | 20711894 |
| coenzyme b12-dependent diol dehydratase is a potassium ion-requiring calcium metalloenzyme: evidence that the substrate-coordinated metal ion is calcium. | the x-ray analyses of coenzyme b(12)-dependent diol dehydratase revealed two kinds of electron densities that correspond to metal ions in the active site. one is directly coordinated by substrate [shibata, n., et al. (1999) structure 7, 997-1008] and the other located near the adenine ring of the coenzyme adenosyl group [masuda, j., et al. (2000) structure 8, 775-788]. both have been assigned as potassium ions, although the coordination distances of the former are slightly shorter than expected. ... | 2010 | 20712378 |
| seasonal variation, chemical composition, and analgesic and antimicrobial activities of the essential oil from leaves of tetradenia riparia (hochst.) codd in southern brazil. | the seasonal variation of the chemical composition of the essential oil from fresh leaves of tetradenia riparia (hochst.) codd grown in southern brazil was analyzed by gc-ms, and the analgesic and antimicrobial activities of this oil were assayed. the yield of essential oil ranged from 0.17% to 0.26%, with the maximum amount in winter and the minimum in spring. the results obtained from principal components analysis (pca) revealed the existence of high chemical variability in the different seaso ... | 2010 | 20714310 |
| predominance of ctx-m genotype among extended spectrum beta lactamase isolates in a tertiary hospital in saudi arabia. | to determine the molecular characterization of extended-spectrum beta-lactamases (esbl) isolates from a tertiary center in saudi arabia using multiplex polymerase chain reaction (pcr) technique and assess their antibiotic susceptibility pattern. | 2010 | 20714681 |
| efficacy and safety of moxifloxacin in uncomplicated pelvic inflammatory disease: the monalisa study. | to evaluate the efficacy and safety of moxifloxacin versus levofloxacin plus metronidazole in uncomplicated pelvic inflammatory disease (upid) in asia. | 2010 | 20716255 |
| antibiotic producing potentials of three freshwater actinomycetes isolated from the eastern cape province of south africa. | crude extracts of three actinomycetes species belonging to saccharopolyspora (tr 046 and tr 039) and actinosynnema (tr 024) genera were screened for antibacterial activities against a panel of several bacterial strains. the extracts showed antibacterial activities against both gram-negative and gram-positive test bacteria with inhibition zones ranging from 8 to 28 mm (tr 046); 8 to15 mm (tr 039); and 10 to 13 mm (tr 024). the minimum inhibitory concentrations ranged from 0.078 to 10 mg/ml (tr 04 ... | 2010 | 20717525 |
| outbreak of ctx-m-15-producing klebsiella pneumoniae of sequence type 199 in a latvian teaching hospital. | previous studies on the epidemiology of extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae in latvia are lacking. esbl-producing klebsiella pneumoniae (n = 32) were subjected to pulsed-field gel electrophoresis (pfge) and selected isolates to multi-locus sequence typing (mlst). species identification and susceptibility testing were performed using vitek2, and sequencing of bla(ctx-m) was performed in selected isolates. pfge revealed one major clone (n = 23), with most of the is ... | 2010 | 20718724 |
| successful pelvic abscess drainage by cecal biopsies in crohn's disease. | | 2011 | 20719310 |
| ctx-m1 esbl-producing klebsiella pneumoniae subsp. pneumoniae isolated from cases of bovine mastitis. | | 2010 | 20720020 |
| toxicological effects of selective herbicides on plant growth promoting activities of phosphate solubilizing klebsiella sp. strain ps19. | this study examines the effect of four herbicides, quizalafop-p-ethyl, clodinafop, metribuzin and glyphosate, on plant growth promoting activities like phosphate solubilization, siderophores, indole acetic acid, exo-polysaccharides, hydrogen cyanide and ammonia production by herbicide tolerant klebsiella sp. strain ps19. the strain was isolated from mustard rhizosphere. the selected herbicides were applied two to three times at the recommended rates. klebsiella sp. strain ps19 tolerated a concen ... | 2011 | 20721665 |
| carbapenems in the treatment of intra-abdominal infection. report from the 20th european congress of clinical microbiology and infectious diseases. vienna, austria, 10-13 april 2010. | the changing pattern of resistance to antibacterial agents was a major theme at the 20th european congress of clinical microbiology and infectious diseases. this article highlights presentations on the increasing threat from multidrug-resistant gram-negative pathogens, particularly extended-spectrum beta-lactamase-producing escherichia coli and klebsiella pneumoniae, which are limiting the choice of drug therapy for serious infections. surveillance data suggest that carbapenem antibiotics are ap ... | 2010 | 20722595 |
| prospective study of urinary tract infection surveillance after kidney transplantation. | urinary tract infection (uti) remains one of the main complications after kidney transplantation and it has serious consequences. | 2010 | 20723254 |
| [changes of pathogens for nosocomial infection of patients with hematological diseases]. | in order to investigate the distribution of nosocomial infection in patients with hematological diseases in our hospital, and to explore the changes of the pathogens isolated. the method of retrospective investigation and analysis was employed. 1164 strain pathogens were isolated from the patients with hematological diseases during the period of 1997-2009. the results showed that the gram-positive cocci infection increased gradually during the 13 years, but has been stable in the last 4 years. t ... | 2010 | 20723323 |
| [analysis of drug resistance and risk factors of enterobacteriaceae in burn units]. | to study the risk factors of infection of extended-spectrum beta-lactamases (esbl)-producing strains and drug resistance of enterobacteriaceae that infected burn patients. | 2010 | 20723421 |
| isolation of a vim-1 metallo-β-lactamase-producing klebsiella pneumoniae isolate in denmark. | | 2010 | 20724117 |
| enhanced h2 gas production from bagasse using adhe inactivated klebsiella oxytoca hp1 by sequential dark-photo fermentations. | sequential dark-photo fermentations (sdpf) was used for hydrogen production from bagasse, an acetaldehyde dehydrogenase (adhe) gene inactivated klebsiella oxytoca hp1 (deltaadhe hp1) mutant was used to reduce the alcohol content in dark fermentation (df) broths and to further enhance the hydrogen yield during the photo fermentation (pf) stage. compared with that of the wild strain, the ethanol concentration in df broths of deltaadhe hp1 decreased 69.4%, which resulted in a hydrogen yield in the ... | 2010 | 20724146 |
| multidrug-resistant klebsiella pneumoniae outbreak after endoscopic retrograde cholangiopancreatography. | infection is a recognized complication of endoscopic retrograde cholangiopancreatography (ercp). we describe the epidemiologic and molecular investigations of an outbreak of ercp-related severe nosocomial infection due to klebsiella pneumoniae producing extended-spectrum beta-lactamase (esbl). | 2010 | 20725887 |
| in vitro ciprofloxacin resistance profiles among gram-negative bacteria isolated from clinical specimens in a teaching hospital. | to evaluate the trends of ciprofloxacin resistance pattern in commonly isolated gram-negative bacteria over time in a saudi arabian teaching hospital. | 2010 | 20726190 |
| microbial transformation of albendazole. | screening scale studies were performed to biotransform anthelmintic drug albendazole by using twelve bacterial strains representing six genera and five actinomycetes cultures. among the cultures studied, bacillus subtilis mtcc 619, escherichia coli mtcc 118 and klebsiella pneumoniae mtcc 109 could transform albendazole to one metabolite whereas, enterobacter aerogenes ncim 2695, klebsiella aerogenes ncim 2258, pseudomonas aeruginosa ncim 2074 and streptomyces griseus ncim 2622 could transform al ... | 2010 | 20726341 |
| uvrb gene deletion enhances sos chromotest sensitivity for nitroreductases that preferentially generate the 4-hydroxylamine metabolite of the anti-cancer prodrug cb1954. | cb1954 is an anti-cancer prodrug that can be reduced at either of two nitro groups to form cytotoxic metabolites. we describe here two efficient and previously uncharacterized nitroreductases, yfko from bacillus subtilis which reduces cb1954 exclusively at the 4-no(2) position, and nfsa from klebsiella pneumoniae which preferentially reduces the 2-no(2) group. utilizing these novel enzymes, together with three previously characterized nitroreductases, we demonstrate that the escherichia coli sos ... | 2010 | 20727918 |
| epidemiology and antimicrobial susceptibility profiles of aerobic and facultative gram-negative bacilli isolated from patients with intra-abdominal infections in the asia-pacific region: 2008 results from smart (study for monitoring antimicrobial resistance trends). | during 2008, the study for monitoring antimicrobial resistance trends (smart) collected 2370 unique aerobic and facultative gram-negative bacilli associated with intra-abdominal infections (iais) from 32 centres in 11 countries in the asia-pacific region and tested their in vitro susceptibility to 12 antimicrobial agents using the clinical and laboratory standards institute (clsi) broth microdilution method. enterobacteriaceae comprised 88.1% of the isolates, of which escherichia coli was the mo ... | 2010 | 20728316 |
| medicinal potions used against infectious bowel diseases in mayan traditional medicine. | since one of the main health problems of the indigenous population are infectious bowel diseases, we decided to test mayan medicinal potions used to treat these conditions against some of the causal agents. | 2010 | 20728517 |
| screening for subgingival occurrence of gram-negative enteric rods in periodontally diseased and healthy subjects. | this study aimed to determine whether the variations in the occurrence of gram-negative enteric rods as detected through a longitudinal follow up with three consecutive visits, may be associated with the periodontal clinical status. | 2010 | 20728867 |
| [a case of subcapsular liver abscess secondary to perforating ulcer of gastric cancer]. | intrahepatic abscess is an unusual complication of peptic ulcer disease. we present a case of gastric cancer in which the ulcer penetrated into the left lobe of liver with subsequent abscess and fistula formation. esophagogastroduodenoscopy confirmed ulcers and a fistula opening in the antrum. abdominal computed tomogram showed a subcapsular liver abscess adjacent to the gastric antrum. subtotal gastrectomy with curettage of the fistulous tract was performed. the final diagnosis was the signet r ... | 2010 | 20729623 |
| evaluation of pathogen detection from clinical samples by real-time polymerase chain reaction using a sepsis pathogen dna detection kit. | sepsis is a serious medical condition that requires rapidly administered, appropriate antibiotic treatment. conventional methods take three or more days for final pathogen identification and antimicrobial susceptibility testing. we organized a prospective observational multicenter study in three study sites to evaluate the diagnostic accuracy and potential clinical utility of the septifast system, a multiplex pathogen detection system used in the clinical setting to support early diagnosis of bl ... | 2010 | 20731880 |
| clinical and microbiological characteristics of klebsiella pneumoniae isolates causing community-acquired urinary tract infections. | klebsiella pneumoniae is the second most common species causing urinary tract infections (uti). however, the host factors and virulence genes of k. pneumoniae related to uti are poorly understood. the aim of this study was to analyze the capsular phenotype and virulence genes of k. pneumoniae isolates and host factors potentially relevant to community-acquired uti. | 2010 | 20734217 |
| chemical composition of fatty acid and unsaponifiable fractions of leaves, stems and roots of arbutus unedo and in vitro antimicrobial activity of unsaponifiable extracts. | the chemical composition of the fatty acid and unsaponifiable fractions of the leaves, stems and roots of arbutus unedo l. were determined using gas chromatography and gas chromatography-mass spectrometry. the fatty acid fractions of the leaves, stems and roots contained 38.5%, 31.3% and 14.1% palmitic acid, respectively, along with other long-chain fatty acids (up to c22). the chemical composition of the unsaponifiable fractions differed: the leaf and stem fractions contained high levels of ali ... | 2010 | 20734946 |
| worldwide diversity of klebsiella pneumoniae that produce beta-lactamase blakpc-2 gene. | klebsiella pneumoniaeisolates that produce carbapenemases (kpcs) are rapidly disseminating worldwide. to determine their genetic background, we investigated 16 blakpc-2-harboring k. pneumoniae isolates from 5 countries. the isolates were multidrug resistant, possessed the blakpc-2 gene, and differed by additional beta-lactamase content. they harbored a naturally chromosome-encoded bla gene (blashv-1 [12.5%], blashv-11 [68.7%], or blaokp-avb [18.8%]) and several acquired and plasmid-encoded genes ... | 2010 | 20735917 |
| invasive klebsiella pneumoniae infections, california, usa. | | 2010 | 20735943 |
| interaction of pqqe and pqqd in the pyrroloquinoline quinone (pqq) biosynthetic pathway links pqqd to the radical sam superfamily. | pqqd is one of six genes required for pqq production in klebsiella pneumoniae. herein, we demonstrate that pqqd interacts specifically with the radical sam enzyme pqqe, causing a perturbation in the electronic environment around the [4fe-4s](+) clusters. this interaction redirects the role for pqqd in pqq biosynthesis. | 2010 | 20737074 |
| [domestic chromogenic nutrient medium for differentiation of klebsiella]. | studies to design a dry chromogenic nutrient medium for the diferentiation of klebsiella were under way, by detecting the intracellular klebsiella genus-specific enzyme of human potential pathogenicity--5-aminosalicylate decarboxylase. the composition of the proposed medium that ensured its high sensitivity and improved its differentiating properties as compared with the known traditional media was worked through. klebsiella are isolated and identified on the proposed medium in one step, which s ... | 2010 | 20737678 |
| [effect of methods of application of bacterial ligands on activation of lymphoid organs' effectors in mice]. | evaluation of differentiation dynamics and localization of various populations of lymphocytes as well as their expression of tlrs during different methods of administration (intranasal, oral, and subcutaneous) of bacterial ligands of opportunistic microorganisms (immunovac-vp-4 vaccine) in experiment on mice. | 2010 | 20737684 |
| a new laboratory cultivation of paramecium bursaria using non-pathogenic bacteria strains. | in most studies dealing with the laboratory cultivation of paramecia (paramecium bursaria), klebsiella pneumoniae bacteria are used to inoculate the medium. however, klebsiella pneumoniae is a typical pathogen, and its use is always associated with a risk of infection. the aim of the present research was to examine non-pathogenic bacteria strains as components of the medium for paramecium bursaria. the paramecia were incubated on lettuce infusions bacterized with different bacteria strains: baci ... | 2010 | 20737917 |
| successful eradication of a monoclonal strain of klebsiella pneumoniae during a k. pneumoniae carbapenemase-producing k. pneumoniae outbreak in a surgical intensive care unit in miami, florida. | we describe the investigation and control of a klebsiella pneumoniae carbapenemase-producing k. pneumoniae outbreak in a 20-bed surgical intensive care unit during the period from january 1, 2009 through january 1, 2010. nine patients were either colonized or infected with a monoclonal strain of k. pneumoniae. the implementation of a bundle of interventions on july 2009 successfully controlled the further horizontal spread of this organism. | 2010 | 20738186 |
| high prevalence of bla(ctx-m) extended spectrum beta-lactamase genes in klebsiella pneumoniae isolates from a tertiary care hospital: first report of bla(shv-12), bla(shv-31), bla(shv-38), and bla(ctx-m-15) in brazil. | the aim of this study was to investigate the presence and prevalence of bla(tem), bla(shv), and bla(ctx-m) and bla(ges)-like genes, responsible for extended spectrum beta-lactamases (esbls) production in clinical isolates of klebsiella pneumoniae collected from a brazilian tertiary care hospital. sixty-five esbl producing k. pneumoniae isolates, collected between 2005 and 2007, were screened by polymerase chain reaction (pcr). identification of bla genes was achieved by sequencing. genotyping of ... | 2011 | 20795871 |
| neonatal community-acquired pneumonia: pathogens and treatment. | to analyse the bacterial pathogens and drug sensitivities for neonatal community-acquired pneumonia. | 2010 | 20796185 |
| leukotrienes produced in allergic lung inflammation activate alveolar macrophages. | it has been well-documented that leukotrienes (lts) are released in allergic lung inflammation and that they participate in the physiopathology of asthma. a role for lts in innate immunity has recently emerged: cys-lts were shown to enhance fcgammar-mediated phagocytosis by alveolar macrophages (ams). thus, using a rat model of asthma, we evaluated fcgammar-mediated phagocytosis and killing of klebsiella pneumoniae by ams. the effect of treatment with a cys-lt antagonist (montelukast) on macroph ... | 2010 | 20798516 |
| suprathel-acetic acid matrix versus acticoat and aquacel as an antiseptic dressing: an in vitro study. | the treatment of burn wounds is still a challenge regarding the management of antiseptic wound conditioning. especially, in the united states, silver-containing dressings, such as acticoat and aquacel are frequently used. because silver-containing dressings have well-known drawbacks such as an antimicrobial lack against pseudomonas aeruginosa, we sought to develop an alternative dressing method. in previous studies, we could demonstrate the excellent antiseptic properties of acetic acid against ... | 2010 | 20798627 |
| inhibitory effect of the essential oil from chamaecyparis obtusa on the growth of food-borne pathogens. | in this study, the antibacterial activity of essential oil from chamaecyparis obtusa (sieb. et zucc) leaves and twigs was investigated. the test strains were klebsiella pneumoniae, listeria monocytogenes, salmonella typhimurium, staphylococcus aureus, escherichia coli, legionella pneumophila, and methicilline-resistant staphylococcus aureus. antibacterial activity was estimated by measuring bacterial growth inhibition. histopathological examination was also performed. c. obtusa oil distinctly in ... | 2010 | 20799092 |
| [immunovac-vp4 vaccine used in complex allergen-specific immunotherapy of patients with hay fever]. | assessment of clinical and immunological effects of use of bacterial therapeutic polycomponent vaccine immunovac-vp4 for allergen-specific immunotherapy (asit) of hay fever. | 2010 | 20799399 |
| role of periodontitis in hospital-acquired pneumonia. | this study evaluated the role of periodontal pathogens in 50 hospitalized patients with hospital acquired pneumonia compared with 30 healthy controls. specimens of oropharyngeal aspirate, dental plaque bronchoalveolar lavage and blood cultured 1 or more pathogens in around 80% of patients, predominatel staphylococcus aureus, followed by coagulase-negative staphylococci, streptococcus pneumoniae and klebsiell pneumoniae. antibiotic sensitivity patterns showed concordance of bacterial cultures f ... | 2010 | 20799559 |
| the clinical and prognostic importance of positive blood cultures in adults. | bloodstream infections are a major cause of morbidity and mortality in adults. bloodstream infections should be reassessed periodically because of increased antibiotic resistance, more patients receiving immunomodulatory therapy, improved antiretroviral therapy, and acquisition of infection in health care settings other than hospitals. | 2010 | 20800151 |
| impact of extended spectrum beta-lactamase producing klebsiella pneumoniae infections in severely burned patients. | significantly higher mortality has been demonstrated in patients who suffer severe burns complicated by klebsiella pneumoniae bacteremia. the specific virulence mechanisms associated with this organism in this population are unclear. | 2010 | 20800197 |
| preservation of viability and antibacterial activity of lactobacillus spp. in calcium alginate beads. | the objective of the study was to produce calcium alginate beads able to deliver lactobacillus spp. (lactobacillus plantarum, lactobacillus rhamnosus gg, lactobacillus bulgaricus and lactobacillus lactis) with preserved viability and antibacterial activity. four types of beads, containing entrapped (e), surface and entrapped (es), surface (s) and concentrated surface and entrapped lactobacilli (c(es)) were prepared and physically characterized. the antibacterial activity of lactobacilli cultures ... | 2010 | 20800681 |
| sensitization of microcin j25-resistant strains by a membrane-permeabilizing peptide. | microcin j25 (mccj25) is a plasmid-encoded, 21-amino-acid, antibacterial peptide produced by escherichia coli. mccj25 inhibits rna polymerase and the membrane respiratory chain. mccj25 uptake into e. coli-sensitive strains is mediated by the outer membrane receptor fhua and the inner membrane proteins tonb, exbb, exbd, and sbma. this peptide is active on some e. coli, salmonella, and shigella species strains, while other gram-negative bacteria, such as clinical isolates of enterobacter cloacae, ... | 2010 | 20802073 |
| multiresistant gram-negative infections: a global perspective. | multiresistant gram-negative infections are an increasing problem in hospitals and healthcare facilities worldwide. while much attention has been paid to gram-positive pathogens such as methicillin-resistant staphylococcus aureus and vancomycin-resistant enterococcus lately, the importance of gram-negative nosocomial infections has also been recognized globally. | 2010 | 20802331 |
| construction of stress-induced metabolic pathway from glucose to 1,3-propanediol in escherichia coli. | 1,3-propanediol is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive synthesis. the aim of this study was to engineer an escherichia coli strain that can produce 1,3-propanediol directly from glucose. we successfully constructed a stress-induced metabolic pathway from glucose to 1,3-propanediol in recombinant e. coli by the expression of gpd1 and gpp2 genes from saccharomyces cerevisiae and dha operon from klebsiella pneumoni ... | 2011 | 20803136 |
| prevalence of antimicrobial-resistant pathogens in canadian hospitals: results of the canadian ward surveillance study (canward 2008). | a total of 5,282 bacterial isolates obtained between 1 january and 31 december 31 2008, inclusive, from patients in 10 hospitals across canada as part of the canadian ward surveillance study (canward 2008) underwent susceptibility testing. the 10 most common organisms, representing 78.8% of all clinical specimens, were as follows: escherichia coli (21.4%), methicillin-susceptible staphylococcus aureus (mssa; 13.9%), streptococcus pneumoniae (10.3%), pseudomonas aeruginosa (7.1%), klebsiella pneu ... | 2010 | 20805395 |
| synthesis and in vitro antimicrobial activity of novel n-(6-chlorobenzo[d]thiazol-2-yl) hydrazine carboxamide derivatives of benzothiazole class. | in this study, a series of novel 1,2,4-triazolo-[3,4-b]-1,3,4-thiadiazole (6a-g) and 1,3,4-oxadiazole (7a-g, 8) were synthesized from n-(6-chlorobenzo[d]thiazol-2-yl) hydrazine carboxamide derivatives of benzothiazole class. antimicrobial properties of the title compound derivatives were investigated against one gram (+) bacteria (staphylococcus aureus), three gram (-) bacteria (escherichia coli, pseudomonas aeruginosa, klebsiella pneumoniae) and five fungi (candida albicans, aspergillus niger, ... | 2011 | 20807086 |
| risk factors for and outcomes associated with clinical isolates of escherichia coli and klebsiella species resistant to extended-spectrum cephalosporins among patients admitted to canadian hospitals. | clinical features associated with gram-negative bacterial isolates with extended-spectrum beta-lactamase (esbl)- and ampc-mediated resistance identified in canadian hospitals is largely unknown. the objective of the present study was to determine the demographics, risk factors and outcomes of patients with esbl- or ampc-mediated resistant organisms in canadian hospitals. | 2009 | 20808455 |
| large-scale extraction of recombinant proteins from bacteria. | bacteria are particularly convenient for producing recombinant proteins for purification purposes. suitable extraction methods for bacterial cells include sonication, glass bead milling, grinding with alumina or sand, high-pressure shearing using the french pressure cell (french press), and lysozyme treatment. these procedures are applicable for preparing extracts from a variety of gram-negative bacteria such as escherichia coli and klebsiella pneumoniae, and gram-positive bacteria such as bacil ... | 2010 | 20810631 |
| clinical features and outcome of community-acquired bacterial meningitis in adult patients with liver cirrhosis. | this study aimed to analyze the clinical features, causative pathogens and therapeutic outcomes of bacterial meningitis in patients with liver cirrhosis. | 2010 | 20811273 |
| unusual cause of thyroid abscess. | thyroid abscess is a rare condition of the thyroid gland. the common causative organisms responsible for thyroid abscess are staphylococci and streptococci species. we described a case of thyroid abscess due to klebsiella pneumoniae in an infant. the patient was successfully treated with open surgical drainage and appropriate antimicrobial agents. | 2010 | 20811534 |
| effect of aeration strategy on the metabolic flux of klebsiella pneumoniae producing 1,3-propanediol in continuous cultures at different glycerol concentrations. | the microbial production of 1,3-propaneidol (1,3-pd) by klebsiella pneumoniae in continuous fermentation was investigated under low, medium and high glycerol concentrations in the absence and presence of oxygen. the production of 1,3-pd increased with increasing glycerol concentrations, reaching a maximum (266 mmol l(-1)) under high glycerol concentration (760 mmol l(-1)) with air sparging at 0.04 vvm. the yield of 1,3-pd, however, decreased gradually with increasing glycerol concentrations, wit ... | 2010 | 20811802 |
| [upper urinary tract infections in adults: about 261 episodes]. | upper urinary tract infections are frequent. escherichia coli is the main pathogen identified from community acquired infections. | 2010 | 20812174 |
| [urinary tract infections in children following renal transplantation: a single-center experience]. | urinary tract infection (uti) is the most common complication after kidney transplantation and represents a potential life-threatening risk for the immunocompromised child. | 2010 | 20812176 |
| complications of intracanalicular plugs: a case series. | intracanalicular plugs are commonly used in the management of dry eyes. the authors report 3 cases of complications associated with intracanalicular plugs. | 2010 | 20812830 |
| are elderly end-stage renal disease patients more susceptible for drug resistant organisms in their sputum? | end stage renal disease (esrd) patients are at risk for pneumonia in view of their impaired immune status. similar empiric antibiotic regimens are used in elderly as well as young esrd patients with respiratory tract infections. we conducted an observational, cross sectional study between june 2007 and june 2008 in 100 esrd patients half being > 65 yrs. all patients had positive sputum culture and chest x-ray findings of pneumonia streptococcus pneumoniae was the commonest in younger while klebs ... | 2010 | 20814127 |
| wound contraction effects and antibacterial properties of tualang honey on full-thickness burn wounds in rats in comparison to hydrofibre. | full-thickness burn wounds require excision and skin grafting. multiple surgical procedures are inevitable in managing moderate to severe full-thickness burns. wound bed preparations prior to surgery are necessary in order to prevent wound infection and promote wound healing. honey can be used to treat burn wounds. however, not all the honey is the same. this study aims to evaluate the wound contraction and antibacterial properties of locally-produced tualang honey on managing full-thickness bur ... | 2010 | 20815896 |
| opportunistic bacterial infections in breeding colonies of the nsg mouse strain. | spontaneous morbidity primarily affecting female breeders in 3 independent breeding colonies of nsg (nod.cg-prkdc(scid) i12rg(tm1wjl) /szj) mice prompted an investigation to uncover the cause of disease. necropsies were performed on 264 (157 female and 107 male) spontaneously sick, experimentally unmanipulated nsg mice. in sum, 42 mice (15.9%) had acute or chronic renal inflammatory lesions, of which 12 had concurrent histologic evidence of an ascending urinary tract infection. from 94 kidneys c ... | 2011 | 20817888 |
| prevalence of microorganisms of hygienic interest in an organized abattoir in mumbai, india. | the magnitude of food-borne illnesses in india is unknown because of lack of surveillance networks. monitoring the prevalence of food-borne pathogens and indicators of contamination in primary production at abattoirs is imperative for creating a data bank and for effective control of such pathogens before they enter the food chain. | 2010 | 20818094 |
| emergence of ctx-m group 1-esbl producing klebsiella pneumonia from a tertiary care centre in karachi, pakistan. | extended-spectrum beta-lactamase (esbl)-producing klebsiella pneumoniae have been reported previously from pakistan but the genotypic characteristics of these enzymes is not known. hence the aim of the study was first to characterise the genotypic content of these beta-lactamases and secondly to assess the clonal relationship of these isolates. | 2010 | 20818098 |
| nosocomial blood-stream infections from extended-spectrum-beta-lactamase-producing escherichia coli and klebsiella pneumonia from gb pant hospital, new delhi. | nosocomial septicemia due to extended spectrum beta-(beta)-lactamase (esbl) producing klebsiella pneumoniae and escherichia coli are a therapeutic challenge due to resistance. knowledge of disease burden and resistance patterns is required for proper and timely management. we report the prevalence and antimicrobial susceptibility of esbl producing e. coli and k .pneumoniae from septicemia at a tertiary care hospital. | 2010 | 20818104 |
| 1,3-propandiol production by engineered hansenula polymorpha expressing dha genes from klebsiella pneumoniae. | currently, 1,3-propanediol (1,3-pd) is an important chemical widely used in polymer production, but its availability is being restricted owing to its expensive chemical synthesis. a methylotrophic yeast hansenula polymorpha was engineered by expression of dhab1, dhab2, dhab3, dhab(ra1) and dhab(ra2) encoding glycerol dehydratase complex and dhat encoding 1,3-pd oxidoreductase from klebsiella pneumoniae under direction of promoter of glyceraldehyde-3 phosphate dehydrogenase (gapdh). the engineere ... | 2011 | 20820806 |
| sequential introduction of single room isolation and hand hygiene campaign in the control of methicillin-resistant staphylococcus aureus in intensive care unit. | after renovation of the adult intensive care unit (icu) with installation of ten single rooms, an enhanced infection control program was conducted to control the spread of methicillin-resistant staphylococcus aureus (mrsa) in our hospital. | 2010 | 20822509 |