| antibacterial activity of alpinia galanga (l) willd crude extracts. | methanol, acetone and diethyl ether extracts of alpinia galanga have been evaluated against pathogens viz. bacillus subtilis mtcc 2391, enterobacter aerogene, enterobacter cloacae, enterococcus faecalis, escherichia coli mtcc 1563, klebsiella pneumoniae, pseudomonas aeruginosa mtcc 6642, salmonella typhimurium, staphylococcus aureus and streptococcus epidermis using agar well diffusion method. minimum inhibitory concentration (mic) and minimum bactericidal concentration (mbc) of all the extracts ... | 2010 | 20387130 |
| emergence of ctx-m group 1 extended-spectrum beta-lactamase-producing klebsiella pneumoniae strains in the community. | molecular characterization of esbl-producing k. pneumoniae strains isolated from urine of outpatients in zagreb region during the last five years. | 2010 | 20387722 |
| [characterization of esbl-producing escherichia coli and klebsiella pneumoniae strains isolated from urine of nonhospitalized patients in the zagreb region]. | to determine the prevalence of esbl-producing escherichia coli and klebsiella pneumoniae strains isolated from urine of nonhospitalized patients during a three-year period, to determine their antibiotic susceptibility, investigate the transfer of esbl genes with cotransfer of resistance and to characterize isolated beta-lactamases. | 2010 | 20387724 |
| etiology and outcome of community-acquired lung abscess. | anaerobes are the first and streptococcus species the second most common cause of community-acquired lung abscess (cala) in the west. the etiologic pathogens of this disease have changed in taiwan, with klebsiella pneumoniae being reported as the most common cause of cala. | 2010 | 20389050 |
| a survey of veterinary hospitals in nigeria for the presence of some bacterial organisms of nosocomial and zoonotic potential. | this study was undertaken to determine the type and estimate the prevalence of bacterial organisms on contact surfaces of five close-to-patient facilities in three veterinary health care settings within the sokoto metropolis of north-western nigeria. a total of 30 samples (10 from each setting) were collected and analysed using culture, microscopy and biochemical testing. bacterial species isolated from samples in this study included the following: bacillus sp. (27.3%), staphylococcus aureus (15 ... | 2009 | 20391375 |
| potential airborne microbial hazards for workers on dairy and beef cattle farms in egypt. | this study was conducted to determine the concentration and frequency distribution of certain airborne micro-organisms on cattle farms and their potential health hazards to farm workers. the samples (60 air samples and 240 hand and nasal swabs from cattle farm workers) were collected from ten cattle farms (five dairy barns and five beef sheds) located in the sharkia governorate of egypt. air samples were collected for microbiological examination in liquid media using an all-glass impinger wherea ... | 2009 | 20391378 |
| [characteristics of biodegradation of triphenyltin by enzyme obtained from klebsiella pneumoniae]. | the objective of this study is to illuminate the mechanism of biodegradation of triphenyltin (tpht). the removal of tpht by klebsiella pneumoniae was, therefore, investigated through characteristics studies. the influences of the various parameters were also discussed. the results demonstrated that the cell, extracellular secretion and intracellular enzyme were the effective biomasses for the biodegradation of tpht. at initial concentration of 3 mg x l(-1), 10.9%, 5.3% and 47.9% of tpht could be ... | 2010 | 20391718 |
| crystal structure of klebsiella sp. asr1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme. | the extracellular phytase of the plant-associated klebsiella sp. asr1 is a member of the histidine-acid-phosphatase family and acts primarily as a scavenger of phosphate groups locked in the phytic acid molecule. the klebsiella enzyme is distinguished from the escherichia coli phytase appa by its sequence and phytate degradation pathway. the crystal structure of the phytase from klebsiella sp. asr1 has been determined to 1.7 a resolution using single-wavelength anomalous-diffraction phasing. des ... | 2010 | 20392204 |
| hospital-acquired conjunctivitis in a neonatal intensive care unit: bacterial etiology and susceptibility patterns. | our study investigates the causative pathogens of hospital-acquired conjunctivitis in our neonatal intensive care unit and their susceptibility patterns. coagulase-negative staphylococcus was the most common bacterium, 22.1% of all isolates. the frequency of the pathogens changed during neonates' stay; klebsiella pneumoniae (from 18% to 6.9%) and escherichia coli (from 16% to 4.8%) decreased, whereas methicillin-susceptible staphylococcus aureus (from 4% to 12.7%) and enterococcus spp (from 1% t ... | 2010 | 20392539 |
| characterisation of the ctx-m-15-encoding gene in klebsiella pneumoniae strains from the barcelona metropolitan area: plasmid diversity and chromosomal integration. | the localisation and genetic organisation of bla(ctx-m-15) were studied in 37 ctx-m-15-producing klebsiella pneumoniae isolates collected from 2005 to 2008 within the barcelona metropolitan area. polymerase chain reaction (pcr)-based replicon typing and southern hybridisations were used to identify the bla(ctx-m-15) location. the genetic environment was analysed by pcr mapping and sequencing, and transferability of bla(ctx-m-15) was evaluated by conjugation and transformation assays. the majorit ... | 2010 | 20392607 |
| clinical and economic outcomes associated with community-acquired intra-abdominal infections caused by extended spectrum beta-lactamase (esbl) producing bacteria in china. | to compare clinical and economic outcomes in patients with community-acquired intra-abdominal infection (iai) due to extended spectrum beta-lactamase (esbl) producing (esbl-positive) bacteria versus non-esbl-producing (esbl-negative) bacteria in china. | 2010 | 20394469 |
| inhibitor-based methods for the detection of kpc carbapenemase-producing enterobacteriaceae in clinical practice by using boronic acid compounds. | enterobacteriaceae clinical strains that produce the class a carbapenem-hydrolysing enzyme kpc (klebsiella pneumoniae carbapenemase) are increasingly reported worldwide, and are already endemic in north and south america, china, israel and greece. the accurate detection of kpc enzymes is of utmost importance for containing the global spread of kpc producers. currently, the detection of putative carbapenemase production is based on an initial phenotypic screen for carbapenem resistance followed b ... | 2010 | 20395214 |
| atp-binding cassette transporter g1 deficiency dysregulates host defense in the lung. | mice with genetic deletion of the cholesterol efflux transporter, atp-binding cassette (abc) g1, have pulmonary lipidosis and chronic pulmonary inflammation. whether abcg1 regulates host defense is unknown. | 2010 | 20395559 |
| production of enterodiol from defatted flaxseeds through biotransformation by human intestinal bacteria. | the effects of enterolignans, e.g., enterodiol (end) and particularly its oxidation product, enterolactone (enl), on prevention of hormone-dependent diseases, such as osteoporosis, cardiovascular diseases, hyperlipemia, breast cancer, colon cancer, prostate cancer and menopausal syndrome, have attracted much attention. to date, the main way to obtain end and enl is chemical synthesis, which is expensive and inevitably leads to environmental pollution. to explore a more economic and eco-friendly ... | 2010 | 20398397 |
| thalidomide treatment modulates macrophage pro-inflammatory function and cytokine levels in klebsiella pneumoniae b5055 induced pneumonia in balb/c mice. | lung innate immune response plays an important role in the clearance of pathogens from lungs, however, profound activation of innate immune cells (alveolar macrophages or neutrophils) can lead to development of acute lung inflammation or injury by producing various pro-inflammatory molecules (il-1, tnf-alpha and h2o2 etc.). present study is designed to investigate the immunomodulatory action of thalidomide in klebsiella pneumoniae b5055 induced acute lung infection in balb/c mice. acute lung inf ... | 2010 | 20399910 |
| rapid characterization of beta-lactamases by multiplex pcr. | the rising prevalence of the members of the enterobacteriaceae, pseudomonas spp. and acinetobacter baumannii that produce extended-spectrum beta-lactamases (esbls) and carbapenem-hydrolysing beta-lactamases (carbapenemases) represents one of the largest "new" resistance problems faced by clinicians and microbiologists during the last 10 years. these diverse enzymes have emerged globally and represent serious health challenges, compromising therapeutic choice and complicating patient management. ... | 2010 | 20401595 |
| emergence of a colistin-resistant kpc-2-producing klebsiella pneumoniae st258 clone in hungary. | nine klebsiella pneumoniae isolates showing non-susceptibility to carbapenems were collected from three centres in the north-eastern region of hungary. the minimum inhibitory concentrations (mics) of antibiotics were determined by etest. the putative production of a carbapenemase was tested by the modified hodge test. the presence of bla (kpc) genes was verified by polymerase chain reaction (pcr) and sequencing. furthermore, molecular typing was performed by pulsed-field gel electrophoresis (pfg ... | 2010 | 20401676 |
| [rhinoscleroma]. | rhinoescleroma is a chronic infectious granuloma due to klebsiella rhinoscleromatis. the nose is involved in all cases with frequent extension to other parts of the airways. rhinoscleroma is endemic to areas of africa, south-east asia, mexico, central and south america, and central and eastern europe. in the past, rhinoscleroma was infrequent in the spanish population but, with current trends in migration, the incidence of rhinoscleroma may be on the rise. diagnosis is made by direct evidence of ... | 2010 | 20402002 |
| nosocomial infections in neonatal units in turkey: epidemiology, problems, unit policies and opinions of healthcare workers. | the epidemiology of nosocomial infections in turkish neonatal intensive care units (nicus) shows that nosocomial sepsis is an important problem, especially for very low birth weight (vlbw) infants, and gram-negative agents, particularly klebsiella species, are still the major causes of nosocomial infections. sepsis frequency was 6.4%, ranging from 2.1 to 17%, in 16 centers in turkey. sepsis frequency was 22% in infants < 1500 g, 6% in those 1500-2500 g, and 3% in those > 2500 g. sepsis-related m ... | 2010 | 20402067 |
| sequencing and genetic variation of multidrug resistance plasmids in klebsiella pneumoniae. | the development of multidrug resistance is a major problem in the treatment of pathogenic microorganisms by distinct antimicrobial agents. characterizing the genetic variation among plasmids from different bacterial species or strains is a key step towards understanding the mechanism of virulence and their evolution. | 2010 | 20405037 |
| in vitro selection of resistance in escherichia coli and klebsiella spp. at in vivo fluoroquinolone concentrations. | fluoroquinolones are potent antimicrobial agents used for the treatment of a wide variety of community- and nosocomial- infections. however, resistance to fluoroquinolones in enterobacteriaceae is increasingly reported. studies assessing the ability of fluoroquinolones to select for resistance have often used antimicrobial concentrations quite different from those actually acquired at the site of infection. the present study compared the ability to select for resistance of levofloxacin, ciproflo ... | 2010 | 20409341 |
| identification of a blavim-4 gene in the internationally successful klebsiella pneumoniae st11 clone and in a klebsiella oxytoca strain in hungary. | | 2010 | 20410063 |
| a membrane-located glycosyltransferase complex required for biosynthesis of the d-galactan i lipopolysaccharide o antigen in klebsiella pneumoniae. | d-galactan i is a polysaccharide with the disaccharide repeat unit structure [-->3-beta-d-galf-(1-->3)-alpha-d-galp-(1-->]. this glycan represents the lipopolysaccharide o antigen found in many gram-negative bacteria, including several klebsiella pneumoniae o serotypes. the polysaccharide is synthesized in the cytoplasm prior to its export via an atp-binding cassette transporter. sequence analysis predicts three galactosyltransferases in the d-galactan i genetic locus. they are wbbo (belonging t ... | 2010 | 20410291 |
| pathogenicity of aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis to brown tree frogs (litoria ewingii). | bacterial dermatosepticemia, a systemic infectious bacterial disease of frogs, can be caused by several opportunistic gram-negative bacterial species including aeromonas hydrophila, chryseobacterium indologenes, chryseobacterium meningosepticum, citrobacter freundii, klebsiella pneumoniae, proteus mirabilis, pseudomonas aeruginosa, and serratia liquifaciens. here we determined the pathogenicity of 3 bacterial species (aeromonas hydrophila, klebsiella pneumoniae, and proteus mirabilis) associated ... | 2010 | 20412685 |
| [bacterial meningitis due to gram-negative bacilli in adults]. | gram-negative bacilli are causative agents of bacterial meningitis in the neonatal period but rarely cause meningitis in adults without risk factors. | 2010 | 20414871 |
| in vitro assessment of antibacterial activity and cytocompatibility of silver-containing phbv nanofibrous scaffolds for tissue engineering. | infections with bacteria have become a serious problem in joint arthroplasty. this study reports about in vitro antibacterial activity and in vitro cell compatibility of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (phbv) nanofibers loaded with metallic silver particles of a size of 5-13 nm. in vitro antibacterial activity against staphylococcus aureus and klebsiella pneumoniae was studied by microplate proliferation tests. the adhesion, viability, and proliferation properties of fibroblasts (n ... | 2010 | 20415469 |
| [characteristics of urinary tract infections in different patient subpopulations and depending on the bladder emptying system]. | to analyze the prevalence and etiology of urinary tract infection in patients with neurogenic bladder depending on the bladder emptying system used as compared to a population with no neurological impairment. to assess perception of urinary tract infection by patients with neurogenic bladder. | 2010 | 20416242 |
| production and immunogenicity of recombinant ferric enterobactin protein (fepa). | ferric siderophore complexes are produced by most bacteria to acquire iron, a vital element. these complexes are transported across the outer membrane by receptor proteins commonly known as fepa (ferric enterobactin protein). in this study we attempted to evaluate the immunogenicity of the membrane protein fepa, aiming at inhibition of iron uptake to protect invasion of the host by the bacterium. | 2010 | 20418143 |
| abolition of biofilm formation in urinary tract escherichia coli and klebsiella isolates by metal interference through competition for fur. | bacterial biofilms are associated with a large number of persistent and chronic infections. biofilm-dwelling bacteria are particularly resistant to antibiotics and immune defenses, which makes it hard if not impossible to eradicate biofilm-associated infections. in the urinary tract, free iron is strictly limited but is critical for bacterial growth. biofilm-associated escherichia coli cells are particularly desperate for iron. an attractive way of inhibiting biofilm formation is to fool the bac ... | 2010 | 20418434 |
| invasive bacterial infections in neonates and young infants born outside hospital admitted to a rural hospital in kenya. | bacterial sepsis is thought to be a major cause of young infant deaths in low-income countries, but there are few precise estimates of its burden or causes. we studied invasive bacterial infections (ibis) in young infants, born at home or in first-level health units ("outborn") who were admitted to a kenyan rural district hospital during an 8-year period. | 2010 | 20418799 |
| assessing the emergence of resistance: the absence of biological cost in vivo may compromise fosfomycin treatments for p. aeruginosa infections. | fosfomycin is a cell wall inhibitor used efficiently to treat uncomplicated urinary tract and gastrointestinal infections. a very convenient feature of fosfomycin, among others, is that although the expected frequency of resistant mutants is high, the biological cost associated with mutation impedes an effective growth rate, and bacteria cannot offset the obstacles posed by host defenses or compete with sensitive bacteria. due to the current scarcity of new antibiotics, fosfomycin has been propo ... | 2010 | 20419114 |
| electricity generation from glucose by a klebsiella sp. in microbial fuel cells. | as electrochemically active bacteria play an important role in microbial fuel cells (mfcs), it is necessary to get a comprehensive understanding of their electrogenesis mechanisms. in this study, a new electrochemically active bacterium, klebsiella sp. me17, was employed into an "h" typed mfc for electrogenesis, with glucose as the electron donor. the maximum power density was 1,209 mw/m2 at a resistance of 340 omega and the maximum current was 1.47 ma. given the original anode medium, fresh med ... | 2010 | 20419297 |
| cross-linking of trans reentrant loops in the na(+)-citrate transporter cits of klebsiella pneumoniae. | the membrane topology model of the na(+)-citrate transporter cits of klebsiella pneumoniae shows a core of two homologous domains with opposite orientation in the membrane and each containing a so-called reentrant loop. a split version of cits was constructed to study domain interactions and proximity relationships of the putative reentrant loops. split cits retained 50% transport activity of the wild-type version in membrane vesicles. unspecific cross-linking of the purified complex with glutar ... | 2010 | 20420430 |
| incidence and antimicrobial susceptibility of escherichia coli and klebsiella pneumoniae with extended-spectrum beta-lactamases in community- and hospital-associated intra-abdominal infections in europe: results of the 2008 study for monitoring antimicrobial resistance trends (smart). | from 2002 to 2008, there was a significant increase in extended-spectrum beta-lactamase (esbl)-positive escherichia coli isolates in european intra-abdominal infections, from 4.3% in 2002 to 11.8% in 2008 (p < 0.001), but not for esbl-positive klebsiella pneumoniae isolates (16.4% to 17.9% [p > 0.05]). hospital-associated isolates were more common than community-associated isolates, at 14.0% versus 6.5%, respectively, for e. coli (p < 0.001) and 20.9% versus 5.3%, respectively, for k. pneumoniae ... | 2010 | 20421398 |
| isecp1-mediated transposition and homologous recombination can explain the context of bla(ctx-m-62) linked to qnrb2. | bla(ctx-m-62), a c508t variant of bla(ctx-m-3b), was transferred from klebsiella pneumoniae jie137 on a conjugative plasmid together with a class 1 integron containing the dfra12-gcuf-aada2 cassette array, iscr1, and qnrb2. bla(ctx-m-62) lies between intact and rearranged copies of isecp1 in a configuration that can be explained by a combination of transposition and homologous recombination and which also illustrates the ability of isecp1 to mobilize an adjacent gene as part of transposition uni ... | 2010 | 20421399 |
| clonal spread of klebsiella pneumoniae producing cmy-2 ampc-type beta-lactamase in surgical intensive care units. | | 2009 | 20422132 |
| [bacterial multiresistance emergence in south-western romania]. | introduction: european centre for diseases control (ecdc), involved in the surveillance of nosocomial infections (ni) and resistance to antimicrobials (amr) in europe, estimates 4 million in/year, among whom 37,000 deceased, out of which half are determined by multiresistant germs (mdr). a phenomenon encountered more and more often is that of pan-resistance of germs, without the option for an optimal antibiotherapy. the allarming increase of amr is a phenomenon which our country also faces in th ... | 2009 | 20422922 |
| depsides and other polar constituents from origanum dictamnus l. and their in vitro antimicrobial activity in clinical strains. | phytochemical investigation of the polar extracts of the aerial parts of origanum dictamnus afforded 15 secondary metabolites. one new depside was isolated, to which the trivial name salvianolic acid p (1) was given, in addition to the known depsides rosmarinic acid (2) and rosmarinic acid methyl ester (3), as well as two monoterpenes, thymoquinone (4) and thymoquinol 2-o-beta-glucopyranoside (5); two simple phenolic acids, oresbiusin a (6) and e-caffeic acid (7); six flavonoids, namely, apigeni ... | 2010 | 20423097 |
| clinical outcome of empiric antimicrobial therapy of bacteremia due to extended-spectrum beta-lactamase producing escherichia coli and klebsiella pneumoniae. | abstract: | 2010 | 20423493 |
| discrepancy between vitek 2 and the agar disk method in detecting the amoxicillin-clavulanate resistance in a klebsiella pneumoniae isolate. | | 2010 | 20424528 |
| antibiotic resistance in community-acquired urinary tract infections: prevalence and risk factors. | this study aimed to identify the agents causing community-acquired urinary tract infections (cautis) and their resistance patterns and to investigate risk factors for esbl production. | 2010 | 20424552 |
| bacterial contamination of boar semen affects the litter size. | one hundred and fifteen semen samples were collected from 115 different boars from two farms in cuba. the boars belonged to five different breeds. evaluation of the semen sample characteristics (volume, ph, colour, smell, motility of sperm cells) revealed that they meet international standards. the samples were also tested for the presence of agglutinated sperm cells and for bacterial contamination. seventy five percent of the ejaculates were contaminated with at least one type of bacteria and e ... | 2010 | 20427136 |
| bla(ctx-m-2) and bla(ctx-m-28) extended-spectrum beta-lactamase genes and class 1 integrons in clinical isolates of klebsiella pneumoniae from brazil. | twenty-eight klebsiella pneumoniae clinical isolates that exhibited an extended-spectrum cephalosporin-resistance profile from a city in the northeast of brazil were analysed by pcr and dna sequencing in order to determine the occurrence of blactx-m genes and class 1 integrons. we determined the occurrence of the blactx-m-2 gene in six k. pneumoniae isolates and describe the first detection of the blactx-m-28 gene in south america. seven isolates carried class 1 integrons. partial sequencing ana ... | 2010 | 20428675 |
| production and characterisation of monoclonal antibodies specific for escherichia coli o157:h7. | seven monoclonal antibodies (mabs) specific for escherichia coli o157:h7, one of the major causes of haemorrhagic colitis in humans, were produced by immunising balb/c mice with the strain e. coli o157:h7. these monoclonal antibodies do not cross-react with other bacteria such as salmonella enterica serovar typhimurium, e. coli o14, e. coli jm109, s. enterica serovar enteritidis, s. panama, s. saintpaul, s. derby, s. muenchen, s. bredeney, s. hadar, yersinia enterocolitica, proteus vulgaris, shi ... | 2006 | 20429059 |
| scrotal abscess in a newborn: caused by extended-spectrum beta-lactamase-producing klebsiella pneumoniae. | | 2010 | 20431174 |
| dielectric barrier discharge plasma as a novel approach for improving 1,3-propanediol production in klebsiella pneumoniae. | dielectric barrier discharge plasma was used to generate a stable strain of klebsiella pneumoniae (designated to as kp-m2) with improved 1,3-propanediol production. the specific activities of glycerol dehydrogenase, glycerol dehydratase and 1,3-propanediol oxidoreductase in the crude cell extract increased from 0.11, 9.2 and 0.15 u mg(-1), respectively, for wild type to 0.67, 14.4 and 1.6 u mg(-1) for kp-m2. the glycerol flux of kp-m2 was redistributed with the flux to the reductive pathway bein ... | 2010 | 20431910 |
| changing etiology of community-acquired bacterial meningitis in adults: a nationwide multicenter study in korea. | epidemiologic data on the etiologic organisms is important for appropriate empirical antibiotic treatment of bacterial meningitis. we identified the etiologies of community-acquired bacterial meningitis in korean adults and the associated epidemiological factors. a retrospective, multicenter nationwide study was carried out. patients 18 years of age or older with community-acquired bacterial meningitis with a confirmed pathogen were enrolled. demographic, clinical, and microbiological data were ... | 2010 | 20432052 |
| [kinetic mechanisms of glycerol dehydrogenase and 1,3-propanediol oxidoreductase from klebsiella pneumoniae]. | the kinetic mechanisms of two key enzymes in the biotransformation of glycerol to 1,3-propanediol (1,3-pd) by klebsiella pneumoniae, glycerol dehydrogenase (gdh) and 1,3-propanediol oxidoreductase (pdor), was characterized. kinetics on initial velocity and product inhibition revealed that gdh and pdor follow an ordered bi-bi sequential mechanism. kinetic models for gdh and pdor showed that the oxidation reaction catalyzed by gdh was the rate-limiting step in coupled enzymatic reaction when the g ... | 2010 | 20432935 |
| approach of empirical antibiotic treatment: analyzing bacterial resistance of community-acquired bacteremia. | nosocomial bacterial resistance has been advanced and well studied in taiwan, but there were few reports describing the antibiotic susceptibility of community-acquired pathogens. through collecting data from those who received blood cultures in emergency department, we presented an epidemiological study to analyze appropriate empirical therapy for the community-acquired bacteremia in southern taiwan. | 2010 | 20434122 |
| culture-dependent and culture-independent characterization of microorganisms associated with aedes aegypti (diptera: culicidae) (l.) and dynamics of bacterial colonization in the midgut. | in this work we show that the lumen of aedes aegypti midgut is highly colonized by bacteria that were identified by culture-dependent and culture-independent methods. rdna sequences obtained were compared with those from genbank and the main bacterial genera identified were: serratia, klebsiella, asaia, bacillus, enterococcus, enterobacter,kluyvera and pantoea. all genera were identified in midgut except enterobacter that was observed only in eggs. asaia and pantoea were also identified in eggs ... | 2010 | 20434424 |
| pharmacodynamic modeling of intravenous antibiotics against gram-negative bacteria collected in the united states. | in the era of escalating antimicrobial resistance, the choice of effective empiric antimicrobial therapy has become considerably more difficult. in an attempt to improve antimicrobial selection, pharmacodynamic modeling that considers the drug, dose, dosing interval, and duration of infusion is increasingly used as a tool to assist in the clinical decision-making process. | 2010 | 20435246 |
| characterization and molecular epidemiology of extended-spectrum beta-lactamases in clinical isolates of enterobacteriaceae in a tunisian university hospital. | one hundred extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae were recovered from the intensive care unit and the urology ward of the university hospital of sahloul in tunisia between may 2005 and may 2006. the majority of strains showed a high level of resistance to cefotaxime and ceftazidime. double-disk synergy test and e-test strips were used to confirm production of esbls. the molecular analysis revealed that the majority of strains (91%) carried genes encoding ctx-m-15. ... | 2010 | 20438347 |
| antibiotic resistance patterns of bacterial strains isolated from periplaneta americana and musca domestica in tangier, morocco. | flies and cockroaches are two insects in close contact with human beings. they are carriers of human pathogenic bacteria on the external areas of their bodies or in their digestive tracts. this study examines periplaneta americana and musca domestica collected from the residential areas of six districts in tangier, morocco. | 2010 | 20440055 |
| antimicrobial susceptibility patterns of community-acquired uropathogens in tehran, iran. | antibiotic resistance of urinary tract pathogens has increased worldwide. knowledge of the antibiotic resistance patterns of uropathogens in specific geographical locations is an important factor for choosing an appropriate empirical antimicrobial treatment. the aim of this study was to provide information regarding local resistance patterns of urinary pathogens to the commonly used antibiotics in tehran, iran. | 2010 | 20440056 |
| ventilator-associated pneumonia in a tertiary care hospital in india: role of multi-drug resistant pathogens. | ventilator-associated pneumonia (vap) is the most frequent intensive-care-unit (icu)-acquired infection. the aetiology of vap varies with different patient populations and types of icus. | 2010 | 20440059 |
| klebsiella pneumoniae carbapenemase-producing enterobacteriaceae. | carbapenem-resistant enterobacteriaceae, particularly klebsiella pneumoniae, are rapidly emerging as significant health care-associated pathogens. because of the adverse impact that infection caused by these extensively drug-resistant organisms can have on patient outcomes, efforts to prevent further transmission of these pathogens within the health care setting are urgently needed. in this article, the epidemiology, detection, and prevention of carbapenem-resistant enterobacteriaceae are descri ... | 2010 | 20442598 |
| the profile of antibiotics resistance and integrons of extended-spectrum beta-lactamase producing thermotolerant coliforms isolated from the yangtze river basin in chongqing. | the spreading of extended-spectrum beta-lactamases (esbl)-producing thermotolerant coliforms (tc) in the water environment is a threat to human health but little is known about esbl-producing tcs in the yangtze river. we received 319 esbl-producing stains obtained from the chongqing basin and we investigated antibiotic susceptibility, bla gene types and the presence of integrons and gene cassettes. 16.8% of tc isolates were esbl-producing bacteria and bla(tem+ctx-m) was the predominant esbl type ... | 2010 | 20447743 |
| effects of plant growth promoting bacteria and mycorrhizal on capsicum annuum l. var. aviculare ([dierbach] d'arcy and eshbaugh) germination under stressing abiotic conditions. | capsicum annuum var. aviculare to tarahumara and papago indians and farmers of sonora desert is a promising biological and commercial value as a natural resource from arid and semiarid coastal zones. traditionally, apply synthetic fertilizers to compensate for soil nitrogen deficiency. however, indiscriminate use of these fertilizers might increase salinity. the inoculation by plant growth promoting bacteria (pgpb) and arbuscular mycorrhizal fungi (amf) represents an alternative as potential bio ... | 2010 | 20447830 |
| primary klebsiella pneumoniae liver abscess with metastatic spread to lung and eye, a north-european case report of an emerging syndrome. | a syndrome of community acquired liver abscess caused by highly virulent hypermucoviscous, rmpa positive klebsiella pneumoniae strains occurs with high incidence in asia. we here report a case complicated by metastatic infection, to our knowledge, the first one described in northern europe. this and other recently reported cases indicate the emergence of this severe syndrome outside of asia. | 2010 | 20448814 |
| screening of antibacterial potentials of some medicinal plants from melghat forest in india. | cyperus rotundus, caesalpinia bonducella, tinospora cordifolia, gardenia gummifera, ailanthus excelsa, acacia arabica, embelia ribes and ventilago maderspatana from melghat forest were screened for their antibacterial potential against escherichia coli, staphylococcus aureus, klebsiella pneumoniae, proteus vulgaris, salmonella typhi, shigella flexneri, salmonella paratyphi, salmonella typhimurium, pseudomonas aeruginosa, enterobacter aerogenes by disc diffusion method. out of these medicinal pla ... | 2009 | 20448847 |
| qualitative phytochemical screening and in vitro antimicrobial effects of methanol stem bark extract of ficus thonningii (moraceae). | the methanolic stem bark extract of ficus thonningii (moraceae) was subjected to preliminary phytochemical screening and in vitro antimicrobial tests. the phytochemical tests was carried out using standard methods of analysis and these investigations revealed the presence of alkaloids, anthraquinones, carbohydrates, flavonoids, saponins and tannins. the antimicrobial activity of the plant extract was assayed using the agar plate disc diffusion and nutrient broth dilution techniques. test micro o ... | 2009 | 20448855 |
| antimicrobial activity and a sar study of some novel benzimidazole derivatives bearing hydrazone moiety. | in this study 12 novel benzimidazole compounds bearing hydrazone moiety were synthesized in order to investigate their possible antibacterial and antifungal activity. structures of the synthesized compounds were elucidated by spectral data. six different gram-negative and four different gram-positive bacterial strains were used in antibacterial activity tests. antifungal activity tests were also performed against three different fungal strains. most of the test compounds found to be significantl ... | 2010 | 20451306 |
| [molecular epidemiology of carbapenem-resistant klebsiella pneumoniae strains isolated between 2004-2007 in ankara university hospital, turkey]. | the prevalence of carbapenem-resistant gram-negative bacteria in the hospital setting is in an increasing trend worldwide. since most of the carbapenem-resistant enterobacteriaceae are resistant to all antimicrobial agents except polymyxins and tigecycline, the emergence of carbapenem resistance in klebsiella pneumoniae strains requires careful monitoring. this study was conducted to analyse the epidemiological relatedness between the carbapenem-resistant isolates of k. pneumoniae collected from ... | 2010 | 20455393 |
| [the performance of two different chromogenic media for the diagnosis of extended spectrum beta-lactamase producing strains]. | the aim of this study was to evaluate the performances of 2 different chromogenic media for the detection of extended spectrum beta-lactamase (esbl) producing escherichia coli and klebsiella spp. isolated from different clinical samples of patients who were admitted to baskent university faculty of medicine, adana application and research hospital between september to november 2007. a total of 365 strains [251 were esbl positive and 114 were negative by double disc synergy (dds) test] of which 2 ... | 2010 | 20455405 |
| [investigation of the in vitro antibacterial effects of statins]. | it was previously shown that statins have anti-inflammatory, immunomodulatory and anti-oxidant effects. this study was aimed to investigate the in-vitro antibacterial effects of simvastatin and atorvastatin. in this study antibacterial activity of the statins were tested against 16 methicillin-susceptible stophylococcus aureus (mssa), 16 methicillin-resistant s.aureus (mrsa), 16 methicillin-resistant coagulase-negative staphylococcus (mrcons), 9 vancomycin-susceptible enterococcus faecium, 7 van ... | 2010 | 20455414 |
| a simple and effective approach for the treatment of diabetic foot ulcers with different wagner grades. | diabetic foot infections are the major cause of morbidity. infection is the common sequel of diabetic foot ulceration that leads to delayed wound healing. these infections are difficult to control. if not addressed well in time, they may lead to amputation of foot. an attempt has been made to develop simple and effective treatment modality by using citric acid as a sole antimicrobial agent to control diabetic foot infections not responding to conventional treatment. hundred and fifteen cases of ... | 2010 | 20455958 |
| intervention study of the association of antibiotic utilization measures with control of extended-spectrum beta-lactamase (esbl)-producing bacteria. | we investigated the effects of replacing third-/fourth-generation cephalosporins with piperacillin-tazobactam on the rate of acquisition of extended spectrum beta-lactamase-producing klebsiella pneumoniae and escherichia coli by patients hospitalized in a department of respiratory medicine. this 9-month, prospective, non-controlled, intervention study comprised two phases: a 3-month pre-intervention phase (phase i) and a 6-month intervention phase (phase ii), during which the use of third-/forth ... | 2010 | 20457272 |
| emergence of resistant klebsiella pneumoniae in the intestinal tract during successful treatment of klebsiella pneumoniae lung infection in rats. | antibiotic treatment of lung infections may lead to the emergence of resistance in the gut flora. appropriate dosing regimens could mitigate this adverse effect. in gnotobiotic rats harboring intestinal escherichia coli and enterococcus faecium populations, a lung infection by klebsiella pneumoniae was instigated with two different sizes of inoculum to represent an early or a late initiation of antibiotic treatment. the rats were treated with marbofloxacin, an expanded-spectrum fluoroquinolone, ... | 2010 | 20457820 |
| effects of the usa patriot act and the 2002 bioterrorism preparedness act on select agent research in the united states. | a bibliometric analysis of the bacillus anthracis and ebola virus archival literature was conducted to determine whether negative consequences of the uniting and strengthening america by providing appropriate tools required to intercept and obstruct terrorism" (usa patriot) act and the 2002 bioterrorism preparedness act on us select agent research could be discerned. indicators of the health of the field, such as number of papers published per year, number of researchers authoring papers, and in ... | 2010 | 20457912 |
| outbreak of klebsiella pneumoniae producing kpc-2 and shv-12 in a french hospital. | | 2010 | 20460399 |
| high prevalence of extended-spectrum beta-lactamases and plasmid-mediated ampc beta-lactamases in enterobacteriaceae isolated from long-term care facilities in korea. | the aim of this study was to investigate for the first time the prevalence and types of extended-spectrum beta-lactamase (esbl) and plasmid-mediated ampc beta-lactamase (pabl) in enterobacteriaceae in korean long-term care facilities (ltcfs). esbl rates were 45.3% (72/159) in escherichia coli and 42.7% (50/117) in klebsiella pneumoniae. in e. coli, ctx-m-28, the most prevalent esbls, was identified for the first time in korea in 44 isolates. in k. pneumoniae, shv-12 was found in 27 isolates (52. ... | 2010 | 20462727 |
| in vivo acquisition of a plasmid-mediated bla(kpc-2) gene among clonal isolates of serratia marcescens. | three patients admitted to a greek hospital were infected with serratia marcescens isolates that exhibited reduced susceptibility to carbapenems and harbored klebsiella pneumoniae carbapenemase (kpc) enzymes. in two of these cases, the patients were initially infected by carbapenem-susceptible s. marcescens isolates. molecular typing and plasmid analysis suggested that all three patients had clonally indistinguishable isolates of s. marcescens that acquired a plasmid-mediated bla(kpc-2) gene dur ... | 2010 | 20463153 |
| bloodstream infections caused by esbl-producing e. coli and k. pneumoniae: risk factors for multidrug-resistance. | this prospective case-control study was conducted from october 2003 to june 2007 to evaluate risk factors for multidrug resistance among extended-spectrum-b-lactamase-producing escherichia coli and klebsiella spp. (esbl-ek) isolates in blood cultures. all adult patients (>18 years old) whose blood cultures grew esbl-ek during the study period were included. an esbl-ek isolate was defined as mdr if it was resistant to at least one member of following two classes of antibiotics: aminoglycosides (a ... | 2009 | 20464329 |
| cefepime-resistant gram-negative bacteremia in febrile neutropenic patients with hematological malignancies. | this study was performed to determine the local etiologic pattern of blood culture isolates and antibiotic resistance in febrile neutropenic patients with hematological malignancies. | 2010 | 20471883 |
| the filter-feeding ciliates colpidium striatum and tetrahymena pyriformis display selective feeding behaviours in the presence of mixed, equally-sized, bacterial prey. | this study examined whether two ciliates could discriminate between equally-sized bacterial prey in mixture and if so, how selectivity might benefit the ciliate population. live klebsiella aerogenes, k. ozaenae and escherichia coli, expressing different coloured fluorescent proteins, were cultured in such a way as to provide populations containing equally-sized cells (to prevent size-selective grazing taking place) and these prey were fed to each ciliate in 50:50 mixtures. colpidium striatum sel ... | 2010 | 20471910 |
| antibiotic resistance in orthopaedic surgery: acute knee prosthetic joint infections due to extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae. | the aim of this study was to describe the prevalence and characteristics of knee prosthetic joint infections due to extended-spectrum beta-lactamase (esbl)-producing enterobacteriaceae. from 2000 to 2007, 132 infections out of 5,076 arthroplasties (2.6%) were registered. seven out of 132 infections (5.3%) were due to esbl-producing enterobacteriaceae, escherichia coli in six cases and klebsiella pneumoniae in one. open debridement and retention of the implant was the first surgical approach and ... | 2010 | 20473541 |
| production and characterization of a group of bioemulsifiers from the marine bacillus velezensis strain h3. | marine microbes are a rich source of bioactive compounds, such as drugs, enzymes, and biosurfactants. to explore the bioactive compounds from our marine natural product library, an oil emulsification assay was applied to discover biosurfactants and bioemulsifiers. a spore-forming bacterial strain from sea mud was found to produce bioemulsifiers with good biosurfactant activity and a broad spectrum of antimicrobial properties. it was identified as bacillus velezensis h3 using genomic and phenotyp ... | 2010 | 20473663 |
| biofilms on tracheoesophageal voice prostheses: a confocal laser scanning microscopy demonstration of mixed bacterial and yeast biofilms. | the aim of this study was to demonstrate the presence of yeast and bacterial biofilms on the surface of tracheoesophageal voice prostheses (tvps) by a double-staining technique with confocal laser scanning microscopy (clsm). biofilms of 12 removed tvps were visualized by scanning electron microscopy, then stained with cona-fitc and propidium iodide for clsm. microbial identification was by partial 16s rrna gene analysis and its-2 sequence analysis. microbial biofilms on the tvps consisted of bac ... | 2010 | 20473799 |
| activity of chequerboard combinations of ceftaroline and nxl104 versus beta-lactamase-producing enterobacteriaceae. | ceftaroline is a novel oxyimino-cephalosporin, strongly active against methicillin-resistant staphylococci and pneumococci. it is active against enterobacteriaceae too, but is labile to common beta-lactamases, including ampc and extended-spectrum types. to counteract these enzymes, ceftaroline is also being developed combined with nxl104, a beta-lactamase inhibitor. | 2010 | 20478991 |
| a novel polymyxin derivative that lacks the fatty acid tail and carries only three positive charges has strong synergism with agents excluded by the intact outer membrane. | polymyxins are cationic lipopeptides (five cationic charges) and the last resort for the treatment of serious gram-negative infections caused by multiresistant strains. nab741 has a cyclic peptide portion identical to that of polymyxin b but carries in the linear peptide portion a threonyl-d-serinyl residue (no cationic charges) instead of the diaminobutyryl-threonyl-diaminobutyryl residue (two cationic charges). at the n terminus of the peptide, nab741 carries an acetyl group instead of a mixtu ... | 2010 | 20479195 |
| identification of helicobacter pylori in gallstone, bile, and other hepatobiliary tissues of patients with cholecystitis. | bacterial infection is accepted as a precipitating factor in cholesterol gallstone formation, and recent studies have revealed the presence of helicobacter species in the hepatobiliary system. we utilized the polymerase chain reaction (pcr) to establish the presence of bacterial dna, including from helicobacter species, in gallstones, bile juice, and gallbladder mucosa from patients with gallstones. | 2010 | 20479914 |
| bacteriological quality of drinking water in nyala, south darfur, sudan. | the objective of this study was to determine the bacterial contaminations in drinking water in nyala city, south darfur, sudan with special reference to the internally displaced people camps (idps). two hundred and forty water samples from different sites and sources including bore holes, hand pumps, dug wells, water points, water reservoir and household storage containers were collected in 2009. the most probable number method was used to detect and count the total coliform, faecal coliform and ... | 2011 | 20480392 |
| klebsiella pneumoniae bacteraemia complicating rotavirus gastroenteritis in two infants with glucocorticoid deficiency. | rotavirus gastroenteritis was complicated by klebsiella pneumoniae bacteraemia in two infants with glucocorticoid deficient conditions who were treated with 'stress dose' hydrocortisone during their illness. delayed healing in the context of glucocorticoid administration combined with damage from rotavirus infection may result in increased risk of mucosal invasion by gastrointestinal bacteria and subsequent enteric gram-negative bacteraemia. | 2010 | 20480730 |
| in vitro susceptibility to various antibiotics of colistin-resistant gram-negative bacterial isolates in a general tertiary hospital in crete, greece. | | 2010 | 20482269 |
| screening for genes involved in klebsiella pneumoniae biofilm formation using a fosmid library. | klebsiella pneumoniae is a well-known opportunistic pathogen, often causing catheter-associated urinary tract infections. biofilm formation on the catheter surfaces is an important step in the development of these infections. to identify the genes involved in the ability of k. pneumoniae to form a biofilm on abiotic surfaces, a novel strategy was used. a clone library was constructed by cloning the entire k. pneumoniae genome of the clinical isolate c3091 into a fosmid vector and the clone libra ... | 2010 | 20482632 |
| evaluation of microbial contamination associated with different preparation methods for neonatal intravenous fat emulsion infusion. | microbial contamination associated with different methods of neonatal intravenous fat emulsion (ivfe) preparation and delivery was evaluated. | 2010 | 20484215 |
| comparison of meropenem mics and susceptibilities for carbapenemase-producing klebsiella pneumoniae isolates by various testing methods. | we describe the levels of agreement between broth microdilution, etest, vitek 2, sensititre, and microscan methods to accurately define the meropenem mic and categorical interpretation of susceptibility against carbapenemase-producing klebsiella pneumoniae (kpc). a total of 46 clinical k. pneumoniae isolates with kpc genotypes, all modified hodge test and bla(kpc) positive, collected from two hospitals in ny were included. results obtained by each method were compared with those from broth micro ... | 2010 | 20484603 |
| acute pyelonephritis can have serious complications. | urinary tract infection (uti) may predominantly involve the lower urinary tract, i.e. acute cystitis, or upper urinary tract consisting of the renal pelvis and kidney,, i.e. acute pyelonephritis the incidence of acute pyelonephritis is higher in young women than in men but the incidence in men over 65 is similar to that in older women. women have up to a 10% risk of recurrent acute pyelonephritis in the year following a first acute episode. the equivalent risk in men is 6%. acute pyelonephritis ... | 2010 | 20486480 |
| termination of a 4-month serial klebsiella pneumoniae septicemia in a diabetic woman after aneurysmectomy in conjunction with antimicrobial therapy. | a diabetic woman suffered from deep neck infection, endophthalmitis, urinary tract infection, and mycotic aneurysm associated klebsiella pneumoniae bacteremia for 4 months. aneurysmectomy and antibiotic therapy terminated recurrent k pneumoniae sepsis suggestive of removal of the pathogen niche in an artery, which served as the root of serial infections. the dna fingerprints of k pneumoniae isolates indicated that the same strain k pneumoniae caused all the infection episodes. the case is report ... | 2010 | 20488656 |
| cadmium chloride exhibits a profound toxic effect on bacterial microflora of the mice gastrointestinal tract. | cadmium (cd²(+)), a naturally occurring heavy metal, is an important environmental pollutant and a potent toxicant to bacteria. the gastrointestinal (gi) tract microflora has a marked capacity to cope with the increased load of ingested metals. however, heavy metals may have harmful effects on git microflora. under the conditions of experimental exposure to cadmium, changes in the population of intestinal microflora in healthy mice were examined. five experimental groups received 23 to 50 mg kg⁻ ... | 2011 | 20488844 |
| kpc-2 producing klebsiella pneumoniae and escherichia coli co-infection in a catheter-related infection. | we describe the first report of simultaneous blood infection with kpc-2 producing klebsiella pneumoniae and escherichia coli in a brazilian patient. we highlight the importance of implementing efficient infection control measures to limit the spread of these phenotypes in a hospital setting. | 2011 | 20491828 |
| quinazoline derivatives are efficient chemosensitizers of antibiotic activity in enterobacter aerogenes, klebsiella pneumoniae and pseudomonas aeruginosa resistant strains. | amongst the three series of quinazoline derivatives synthesised and studied in this work, some molecules increase the antibiotic susceptibility of gram-negative bacteria presenting multidrug-resistant phenotypes. n-alkyl compounds induced an increase in the activity of chloramphenicol, nalidixic acid and sparfloxacin, which are substrates of the acrab-tolc and mexab-oprm efflux pumps in clinical isolates. these molecules are able to increase the intracellular concentration of chloramphenicol in ... | 2010 | 20494558 |
| positive culture for klebsiella pneumoniae in relevant sputum samples as a predictor of rapidly fatal outcome in septic patients at medical intensive care units. | sixty-five patients with main diagnosis of sepsis, who were directly admitted to the emergency department (ed) and had fatal outcomes after transfer to the medical intensive care units (micu), were included. patients who died within 48 hours of micu transfer were defined as having rapidly fatal outcomes (rfo). the following clinical variables, including diagnosis of infection source; results of blood, sputum, and urine cultures; management for sepsis in the ed and micu and survival time, were an ... | 2010 | 20495991 |
| volatiles emitted from eight wound-isolated bacteria differentially attract gravid screwworms (diptera: calliphoridae) to oviposit. | bovine blood inoculated with bacteria isolated from screwworm [cochliomyia hominivorax (coquerel) (diptera: calliphoridae)]-infested animal wounds was tested as an attractant for oviposition for gravid screwworms. eight species of gram-negative coliform (enterobacteriaceae) bacteria mixed with bovine blood singly or all species combined and incubated for various times produced volatiles that attracted gravid flies in a cage bioassay in varying numbers. in 15-min duration tests, volatiles from fi ... | 2010 | 20496582 |
| cadc regulates cad and tdc operons in response to gastrointestinal stresses and enhances intestinal colonization of klebsiella pneumoniae. | to explore the mechanisms that enable klebsiella pneumoniae to survive in the gastrointestinal tract, we characterized the roles of the cad and tdc operons during the bacterial response to acid and bile stress. | 2010 | 20497056 |
| expression of 1,3-propanediol oxidoreductase and its isoenzyme in klebsiella pneumoniae for bioconversion of glycerol into 1,3-propanediol. | in the klebsiella pneumoniae reduction pathway for 1,3-propanediol (1,3-pd) synthesis, glycerol is first dehydrated to 3-hydroxypropionaldehyde (3-hpa) and then reduced to 1,3-pd with nadh consumption. rapid conversion of 3-hpa to 1,3-pd is one of the ways to improve the yield of 1,3-pd from glycerol and to avoid 3-hpa accumulation, which depends on enzyme activity of the reaction and the amount of reducing equivalents available from the oxidative pathway of glycerol. in the present study, the y ... | 2010 | 20499228 |
| [occurrence of resistant enterobacteriaceae isolated from carriers and infections in pediatric wards of a polish reference hospital]. | study was carried out from june to november 2008 in the group of newborn babies hospitalized at the highly specialized hospital. twenty-six clinical isolates of esbl-positive rods were collected from 24 patients. infections incidence was confirmed on the level 3.4/1000 patientdays (pds), among infection dominated blood infection (41%) and pneumonia (31%). all isolates were analyzed for the presence of genes of the resistance (bla)shv, (bla)tem i (bla)ctx-m by the multiplex pcr amplification. iso ... | 2010 | 20499660 |
| klebsiella pneumoniae-associated vertebral osteomyelitis after laparoscopic cholecystectomy. | we report a case of a patient who underwent elective laparoscopic cholecystectomy and subsequently developed klebsiella pneumoniae-associated vertebral osteomyelitis after 2 months. development of vertebral osteomyelitis after laparoscopic cholecystectomy has never been reported previously. diagnosis was made via magnetic resonance imaging. the patient was successfully treated with intravenous antibiotics and had a complete recovery with no neurologic sequelae. | 2010 | 20499776 |
| may chlamydia trachomatis be an aetiological agent of chronic prostatic infection? | chlamydia trachomatis infection is the most common sexually transmitted bacterial disease. the objective of this study was to establish the presence/absence of c. trachomatis in 98 patients with chronic complaints about the prostate and to evaluate the role of this bacterium in the inflammation of the gland. we performed culture and microscopical examination of pre-massage/post-massage urine and expressed prostatic secretions (eps). in all cases, culture on mccoy cells and polymerase chain react ... | 2010 | 20500746 |
| [antibacterial activity of recombinant thanatin expressed by e.coli er2566]. | to express antibacterial peptide thanatin in the prokaryotic expression system and test its antibacterial activity. | 2010 | 20501392 |