| characteristics of pyogenic liver abscess patients with and without diabetes mellitus. | pyogenic liver abscess (pla) is relatively common in patients with diabetes mellitus (dm), but it is unclear whether there are differences between patients with and without dm. we conducted a study to identify the possible differences and factors that affect fatality. | 2010 | 19826410 |
| liver abscess caused by klebsiella pneumoniae: two case reports. | the present case reports highlight the peculiar aspect of klebsiella pneumoniae liver abscess, an emerging disease in united states and western countries. | 2009 | 19829875 |
| breast milk causing neonatal sepsis and death. | breast milk can occasionally transmit serious viral and bacterial infections to preterm infants. we present three cases of late-onset neonatal sepsis, including one that resulted in death, occurring in preterm infants. the likely source of the microorganisms in all three cases was expressed breast milk. | 2010 | 19832716 |
| emergence of esbl-producing escherichia coli and klebsiella pneumoniae isolates during the years 2000 and 2004 in helsinki, finland. | abstract the molecular epidemiology of 33 e. coli and 81 k. pneumoniae esbl-producing health-care associated and community-acquired isolates collected in the helsinki district during 2000-2004 was investigated. clonality studies, antimicrobial susceptibility and genotyping of the isolates were performed. newly emerging ctx-m-producing e. coli and bla(shv-12)-producing k. pneumoniae were detected. clonal clusters of both species persisted throughout the study period. | 2009 | 19832719 |
| summary trends for the meropenem yearly susceptibility test information collection program: a 10-year experience in the united states (1999-2008). | the meropenem yearly susceptibility test information collection (mystic) program was a global, longitudinal antimicrobial resistance surveillance network of more than 100 medical centers worldwide monitoring the susceptibility of meropenem and selected other broad-spectrum comparator agents. in 1999, and from 2000 through 2008, a total of 10 or 15 united states (usa) medical centers each forwarded 200 nonduplicate clinical isolates from serious infections to a central processing laboratory. over ... | 2009 | 19833471 |
| endogenous endophthalmitis caused by citrobacter koseri. | endogenous endophthalmitis occurs when organisms are hematogenously disseminated in to the eye from a distant focus of infection. the most common isolated organisms that cause endogenous endophthalmitis are klebsiella pneumoniae and escherichia coli. previous reports on endophthalmitis caused by citrobacter species are limited. we present the first case of endogenous endophthalmitis caused by citrobacter koseri bacteremia and renal abscesses. | 2009 | 19834321 |
| mucosal lipocalin 2 has pro-inflammatory and iron-sequestering effects in response to bacterial enterobactin. | nasal colonization by both gram-positive and gram-negative pathogens induces expression of the innate immune protein lipocalin 2 (lcn2). lcn2 binds and sequesters the iron-scavenging siderophore enterobactin (ent), preventing bacterial iron acquisition. in addition, lcn2 bound to ent induces release of il-8 from cultured respiratory cells. as a countermeasure, pathogens of the enterobacteriaceae family such as klebsiella pneumoniae produce additional siderophores such as yersiniabactin (ybt) and ... | 2009 | 19834550 |
| adding to yersinia enterocolitica gene pool diversity: two cryptic plasmids from a biotype 1a isolate. | we report the nucleotide sequence of two novel cryptic plasmids (4357 and 14 662 base pairs) carried by a yersinia enterocolitica biotype 1a strain isolated from pork. as distinguished from most biotype 1a strains, this isolate, designated 07-04449, exhibited adherence to eukaryotic cells. the smaller plasmid pye4449-1 carries five attributable open reading frames (orfs) encoding the first ccda/ccdb-like antitoxin/toxin system described for a yersinia plasmid, a repa-like replication initiation ... | 2009 | 19834620 |
| [expression of acetohydroxyacid synthase isozyme genes ilvbn, ilvgm, ilvih and their resistance to ahas-inhibitor herbicides]. | acetohydroxyacid synthase (ahas) catalyses the first reaction in the pathway for synthesis of the branched-chain amino acids. ahas is the target for sulfonylurea, imidazolinone and other ahas-inhibitor herbicides. herbicides-resistant ahas genes have potential application in plant transgenetic engineering and development of new generation herbicide. the ahas isozyme genes ilvbn, ilvgm and ilvih were cloned from metsulfuron-methyl resistant strain klebsiella sp. hr11 and metsulfuron-methyl sensit ... | 2009 | 19835141 |
| in vitro activity of tigecycline against 2423 clinical isolates and comparison of the available interpretation breakpoints. | mics to tigecycline and 12 antimicrobials were performed by microdilution method, against 2423 nonduplicate pathogens recently isolated in 17 greek hospitals. the food and drug administration (fda) and european committee on antimicrobial susceptibility testing (eucast) criteria were used comparatively for interpretation of tigecycline mics. tigecycline exhibited potent in vitro activity against the majority of the isolates tested. (mic(90) values of 0.5, 1, 2, 0.125, 1, 0.25, 0.125, and 1 mg/l w ... | 2010 | 19836184 |
| [epidemiology of esbl-positive enterobacteriaceae in mantova hospital (italy)]. | in a retrospective study concerning the epidemiology of extended-spectrum beta-lactamase (esbl) positive enterobacteriaceae during 2007-2008 in the wards of the carlo poma hospital in mantova, mercurio surveillance software was used to detect alert microorganisms. our objective was to link the epidemiological data with the type of patient and ward, and to assess the risk factors for such infections in particular nosocomial environments. the study enabled the change in the relative epidemiologica ... | 2009 | 19838088 |
| maternal risk factors in early neonatal sepsis at a tertiary care teaching hospital. | to study the maternal risk factors in blood culture proven cases of early neonatal sepsis, and study the isolates and the sensitivity of these isolates. | 2009 | 19838438 |
| risk factors and outcome in extended spectrum beta-lactamase producing escherichia coli and klebsiella pneumoniae bacteremia. | | 2009 | 19838454 |
| ammonium and phosphate limitation in 1,3-propanediol production by klebsiella pneumoniae. | excretion of 1,3-propanediol (1,3-pd) by k. pneumoniae was compared in ammonium- and phosphate-limited chemostat cultures running with an excess of glycerol. 59 and 43% catabolic flux were directed to 1,3-pd in ammonia-limited cultures and phosphate-limited cultures at dilution rate of 0.1 h(-1), respectively. ammonia-limited fed-batch cultures produced 61 g 1,3-pd l(-1) and a total of 15 g l(-1) organic acid in 36 h. however, phosphate-limited fed-batch cultures excreted 61 g lactate l(-1) and ... | 2010 | 19838630 |
| biodegradation of thiocyanate using co-culture of klebsiella pneumoniae and ralstonia sp. | thiocyanate-degrading microbial co-culture was isolated from thiocyanate-contaminated site and tested for thiocyanate degradation potential and thiocyanate-toxicity tolerance and identified as klebsiella pneumoniae and ralstonia sp. by 16s rdna sequencing. the co-culture was able to degrade thiocyanate with degradation rate of 500 mg l(-1)d(-1) at 2,500 mg l(-1) thiocyanate concentration at ph 6.0 and 37 degrees c following thiocyanate hydrolase pathway. the haldane kinetic model elucidates the ... | 2010 | 19838695 |
| emphysematous pyelonephritis: a case report series of four patients with review of literature. | emphysematous pyelonephritis (epn) is an acute necrotizing infection with gas presence in the kidney, perinephric space, and/or urinary collecting system that carries a bad prognosis. some clinical conditions predispose to this entity, such as diabetes mellitus, urinary tract obstruction, and immune-incompetence. immediate resuscitation, broad-spectrum antibiotics, percutaneous and surgical drainage, and emergent and delayed nephrectomy are therapeutic options that should be applied in a timely ... | 2009 | 19839858 |
| antibacterial, antifungal, and antiviral activities of some flavonoids. | antibacterial and antifungal activities of six plant-derived flavonoids representing two different structural groups were evaluated against standard strains of escherichia coli, pseudomonas aeruginosa, proteus mirabilis, klebsiella pneumoniae, acinetobacter baumannii, staphylococcus aureus, enterococcus faecalis, bacillus subtilis and their drug-resistant isolates, as well as fungi (candida albicans, c. krusei) using the microdilution broth method. herpes simplex virus type-1 and parainfluenza-3 ... | 2010 | 19840899 |
| roles of capsule and lipopolysaccharide o antigen in interactions of human monocyte-derived dendritic cells and klebsiella pneumoniae. | in humans, klebsiella pneumoniae is a saprophytic bacterium of the nasopharyngeal and intestinal mucosae that is also frequently responsible for severe nosocomial infections. two major factors of virulence, capsular polysaccharide (cps) and lipopolysaccharide (lps) o antigen, are involved in mucosal colonization and the development of infections. these bacterial surface structures are likely to play major roles in interactions with the mucosal immune system, which are orchestrated by a network o ... | 2010 | 19841082 |
| attributable hospital cost and length of stay associated with health care-associated infections caused by antibiotic-resistant gram-negative bacteria. | determination of the attributable hospital cost and length of stay (los) are of critical importance for patients, providers, and payers who must make rational and informed decisions about patient care and the allocation of resources. the objective of the present study was to determine the additional total hospital cost and los attributable to health care-associated infections (hais) caused by antibiotic-resistant, gram-negative (gn) pathogens. a single-center, retrospective, observational compar ... | 2010 | 19841152 |
| fixed-dose combination of cefepime plus amikacin (potentox) inhibits pneumonia infection. | pneumonia is a severe infection that causes high morbidity and mortality rate worldwide. it is caused by klebsiella pneumoniae, which generally causes upper respiratory tract infection. in case of such type of infection, levels of oxidant and antioxidant become imbalanced, which may contribute to lung injury. the present study was planned to evaluate the status of oxidant and antioxidant enzyme activities in plasma and lung tissue of pneumonia-infected rats model. animals were randomly distribut ... | 2009 | 19842849 |
| characterization of corrosive bacterial consortia isolated from petroleum-product-transporting pipelines. | microbiologically influenced corrosion is a problem commonly encountered in facilities in the oil and gas industries. the present study describes bacterial enumeration and identification in diesel and naphtha pipelines located in the northwest and southwest region in india, using traditional cultivation technique and 16s rdna gene sequencing. phylogenetic analysis of 16s rrna sequences of the isolates was carried out, and the samples obtained from the diesel and naphtha-transporting pipelines sh ... | 2010 | 19844704 |
| both trif- and myd88-dependent signaling contribute to host defense against pulmonary klebsiella infection. | klebsiella pneumoniae causes extensive lung damage. tlr signaling involves adaptors trif and myd88. however, the relative contribution of trif and myd88 signaling in host defense against pulmonary k. pneumoniae infection has not been elucidated. therefore, we investigated the role of trif and myd88 in k. pneumoniae pneumonia. trif(-/-) mice infected with k. pneumoniae showed impaired survival and reduced bacterial clearance, neutrophil influx, histopathologic evidence of inflammation, and tnf-al ... | 2009 | 19846873 |
| rapid detection of esbl-producing gram-negative bacteria isolated from blood: a reasonable and reliable tool for middle and low resource countries. | delay in appropriate treatment in patients with bacteraemia can increase morbidity, mortality, and health expenditures. we compared the rapid direct test (rdt) designed to detect esbl-producing gram-negative bacteria (gnb) directly from positive blood cultures bottles, with two conventional esbl detection tests: screening and confirmatory disk diffusion assay (sc-dda) and an mic screening and esbl e-test (mic/et). | 2009 | 19848308 |
| nitroreductase ii involved in 2,4,6-trinitrotoluene degradation: purification and characterization from klebsiella sp. cl. | three 2,4,6-trinitrotoluene (tnt) nitroreductases from klebsiella sp. ci have different reduction capabilities that can degrade tnt by simultaneous utilization of two initial reduction pathways. of these, nitroreductase ii was purified to homogeneity by sequential chromatographies. nitroreductase ii is an oxygen-insensitive enzyme and reduces both tnt and nitroblue tetrazolium. the n-terminal amino acid sequence of the enzyme did not show any sequence similarity with those of other nitroreductas ... | 2009 | 19851725 |
| prediction of bacterial proteins carrying a nuclear localization signal and nuclear targeting of hsdm from klebsiella pneumoniae. | nuclear targeting of bacterial proteins is an emerging pathogenic mechanism whereby bacterial proteins can interact with nuclear molecules and alter the physiology of host cells. the fully sequenced bacterial genome can predict proteins that target the nuclei of host cells based on the presence of nuclear localization signal (nls). in the present study, we predicted bacterial proteins with the nls sequences from klebsiella pneumoniae by bioinformatic analysis, and 13 proteins were identified as ... | 2009 | 19851738 |
| development of a method based on surface enhanced laser desorption and ionization time of flight mass spectrometry for rapid identification of klebsiella pneumoniae. | a method based on surface enhanced laser desorption and ionization time of flight mass spectrometry (seldi-tof ms) was developed for the rapid identification of klebsiella pneumoniae by directly applying bacterial colonies without further protein extraction. a total of 40 k. pneumoniae and 114 other related microorganisms isolated clinically were analyzed by seldi-tof ms. an identification model for k. pneumoniae was established by artificial neural networks (anns) with classification accuracy o ... | 2009 | 19851739 |
| [kpc carbapenemases: what is at stake in clinical microbiology?]. | emergence and dissemination of carbapenem resistance in the world represent a significant threat for management of hospital-acquired infections. from the early 2000s, enterobacteriaceae that produce klebsiella pneumoniae carbapenemases (kpc) have initially been reported from the usa and now worldwide, becoming the most important carbapenemase. these kpc producing-bacteria are mostly involved in nosocomial and systemic infections. they are mostly enterobacteriaceae and more rarely pseudomonas aer ... | 2010 | 19854586 |
| dominance of ctx-m-2 and ctx-m-56 among extended-spectrum beta-lactamases produced by klebsiella pneumoniae and escherichia coli isolated in hospitals in paraguay. | | 2009 | 19854861 |
| [aminoglycoside resistance of enterobacteria isolated from urinary infections in surgical departments]. | the aim of the study was to determine the aminoglycosides resistance of enterobacteriaceae strains isolated from urocultures. identification of the germs was performed by the api system (biomerieux) and susceptibility tests was performed by disk-diffusion test (clsi standards) and with api strips. for detecting the resistance to aminoglycosides we used gentamicin, tobramycin, netilmicin and amikacin. from 2500 urine samples we isolated 673 microbial strains, from which 531 were enterobacteriacea ... | 2008 | 19856848 |
| acute appendicitis presenting with klebsiella pneumoniae septicemia due to bacterial translocation. | bacterial translocation (bt) is defined as the passage of viable bacteria from the gastrointestinal tract, across the intestinal wall, to the mesenteric lymph nodes or other extranodal sites and bloodstream. it has been shown in both animal and human studies and has been implicated as a source of sepsis in susceptible patients. herein, a rare case of acute appendicitis in a nonimmunocompromised patient who presented with manifestations of klebsiella pneumoniae septicemia, is described. transloca ... | 2009 | 19857441 |
| klebsiella pneumoniae acrab efflux pump contributes to antimicrobial resistance and virulence. | respiratory infections caused by klebsiella pneumoniae are characterized by high rates of mortality and morbidity. management of these infections is often difficult, due to the high frequency of strains that are resistant to multiple antimicrobial agents. multidrug efflux pumps play a major role as a mechanism of antimicrobial resistance in gram-negative pathogens. in the present study, we investigated the role of the k. pneumoniae acrrab operon in antimicrobial resistance and virulence by using ... | 2010 | 19858254 |
| the in-vitro antimicrobial activity of some medicinal plants against beta-lactam-resistant bacteria. | in effort to identify novel bacterial agents, this study was initiated to evaluate the antimicrobial properties of 17 crude extracts from 12 medicinal plants against beta-lactam-resistant bacteria. | 2009 | 19858568 |
| ventilator-associated nosocomial pneumonia in intensive care units in malaysia. | the outcome indicator of nosocomial infection (ni) in the intensive care unit (icu) is used to benchmark the quality of patient care in malaysia. we conducted a three-year prospective study on the incidences of ventilator-associated pneumonia (vap), risk factors, and patterns of the microorganisms isolated in three icus. | 2009 | 19858572 |
| why hypothetical protein kpn00728 of klebsiella pneumoniae should be classified as chain c of succinate dehydrogenase? | twenty percent of genes that encode for hypothetical proteins from klebsiella pneumoniae mgh78578 were identified, leading to kpn00728 and kpn00729 after bioinformatics analysis. both open reading frames showed high sequence homology to succinate dehydrogenase chain c (sdhc) and d (sdhd) from escherichia coli. recently, kpn00729 was assigned as sdhd. kpn00728 thus remains of particular interest as no annotated genes from the complete genome sequence encode for sdhc. we discovered kpn00728 has a ... | 2009 | 19859792 |
| [bacteria isolated from surgical infections and their susceptibility to antimicrobial agents--special reference to bacteria isolated between april 2007 and march 2008]. | bacteria isolated from infections in abdominal surgery during the period from april 2007 to march 2008 were investigated in a multicenter study in japan, and the following results were obtained. in this series, 707 strains including 24 strains of candida spp. were isolated from 181 (79.0%) of 229 patients with surgical infections. three hundred and ninety-five strains were isolated from primary infections, and 288 strains were isolated from postoperative infections. from primary infections, anae ... | 2009 | 19860320 |
| risk factors and clinical impact of klebsiella pneumoniae carbapenemase-producing k. pneumoniae. | klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae is an emerging pathogen with serious clinical and infection control implications. to our knowledge, no study has specifically examined risk factors for kpc-producing k. pneumoniae or its impact on mortality. | 2009 | 19860564 |
| cross-reaction between proteins of larrea divaricata cav. (jarilla) and proteins of gram-negative bacteria. | larrea divaricata is an abundant plant of northwest of argentina used to treat different pathologies. we aimed to characterize the immunogenicity of proteins from a partially purified crude aqueous extract (jpce) of jarilla. we evaluated the cross reaction between jpce and whole cell-bacterial proteins (w-cbp) of escherichia coli, pseudomonas aeruginosa, proteus vulgaris, and klebsiella pneumoniae using a mouse anti-jpce serum. protein profiles of jpce and w-cbp were analyzed. for jpce, 18 bands ... | 2009 | 19874237 |
| evaluation of new chromogenic substrates for the detection of coliforms. | to evaluate a new range of chromogenic substrates for the detection of beta-galactosidase activity in coliforms and to compare their performance in agar media and broths. | 2010 | 19878523 |
| use of automated repetitive-sequence-based pcr for rapid laboratory confirmation of nosocomial outbreaks. | rapid and reliable diagnosis of genetic relatedness of clinical isolates in microbiologic laboratory is essential in case of nosocomial outbreak investigation. most molecular techniques used to type microorganisms are technically demanding and time consuming. currently repetitive-sequence-based pcr (rep-pcr) technique has been adapted to an automated format on the diversilab system (biomérieux, marcy l'etoile, france). aim of this study was to compare the performance of the diversilab system to ... | 2010 | 19879293 |
| chromium (vi) biotransformation by beta- and gamma-proteobacteria from natural polluted environments: a combined biological and chemical treatment for industrial wastes. | the high solubility of cr(vi) in aqueous systems, together with carcinogenic and mutagenic effects on living organisms, make industrial effluents receive specific treatments for cr(vi) elimination. biotreatments, based on biotransformation of cr(vi) to cr(iii) which is immobilized as cr(oh)(3), are the most effective methods for the removal of cr(vi) concentrations below 2mm. the aim of our study is the application of pure or mixed bacterial cultures for cr(vi) biotransformation followed by chem ... | 2010 | 19879691 |
| synthesis, stereochemistry and antimicrobial studies of novel oxime ethers of aza/diazabicycles. | two series of bicyclic oxime ethers viz, 2,4-diaryl-3-azabicyclo[3.3.1]nonan-9-one o-benzyloximes 13-24 and 2,4,6,8-tetraaryl-3,7-diazabicyclo[3.3.1]nonan-9-one o-benzyloximes 31-36 were synthesized and stereochemistry was established by their spectral (1d and 2d nmr) and crystal studies. synthesized oxime ethers were screened for their in vitro antimicrobial activity against a set of pathogenic bacteria (pseudomonas aeruginosa, staphylococcus aureus, salmonella typhi, escherichia coli and klebs ... | 2009 | 19879756 |
| assessment of microbiological quality of sachet-packaged drinking water in western nigeria and its public health significance. | to assess the microbiological quality of sachet-packaged drinking water in western nigeria and its impact on public health. | 2009 | 19880150 |
| cerebritis: an unusual complication of klebsiella pneumoniae. | cerebritis is part of a continuum of brain infection and is difficult to diagnose. cerebritis caused by klebsiella in immunocompetent adults without predisposing factors such as neurosurgery or penetrating brain injury has not been reported before. we report a case of klebsiella cerebritis in an adult patient with a proven extracranial focus of infection. we suggest considering cerebritis as a differential diagnosis for altered level of consciousness in patients of severe sepsis, even if an extr ... | 2009 | 19881180 |
| colistin and polymyxin b: a re-emergence. | one of the greatest achievements of modern medicine is the development of antibiotics against life-threatening infections, but the emergence of multidrug-resistant (mdr) gram negative bacteria has drastically narrowed down the therapeutic options against them. this limitation has led clinicians to reappraise the clinical application of polymyxins, an old class of cationic, cyclic polypeptide antibiotics. polymyxins are active against selected gram-negative bacteria, including the acinetobacter s ... | 2009 | 19881183 |
| isolation of bacteria causing secondary bacterial infection in the lesions of cutaneous leishmaniasis. | cutaneous leishmaniasis (cl) is a parasitic disease characterized by single or multiple ulcerations. secondary bacterial infection is one of the complications of the disease that can increase the tissue destruction and the resulting scar. | 2008 | 19882011 |
| [a case of empyema due to capnocytophaga sp. and actinomyces israelii]. | a 54-year-old man was admitted to our hospital for investigation of cough, sputum production, and fever of 1 month's duration. his diabetes mellitus was poorly controlled, and his hemoglobin hba1c value was elevated at 10.9%. chest x-ray film and computed tomography scan showed bilateral but predominantly right-sided pleural effusion. aspiration of the pleural fluid from the right-side showed frank pus, and empyema was diagnosed. capnocytophaga sp. and actinomyces israelii were isolated in the p ... | 2009 | 19882914 |
| [antibacterial activity of quinolones against various clinically isolated strains and evaluation of efficacy based on the pharmacokinetics/pharmacodynamics theory]. | we compared the antimicrobial activities of oral quinolones, ciprofloxacin (cpfx), gatifloxacin (gflx), garenoxacin (grnx), levofloxacin (lvfx), moxifloxacin (mflx), norfloxacin (nflx), prulifloxacin (pufx), and tosufloxacin (tflx) using streptococcus pneumoniae, haemophilus influenzae, streptococcus agalactiae, streptococcus pyogenes, extended spectrum beta-lactamase(esbl) producing klebsiella pneumoniae, and methicillin-susceptible staphylococcus aureus (mssa) isolated from clinical materials. ... | 2009 | 19882980 |
| [analysis on the sensitivity to beta-lactam antibiotics of respiratory-infectious isolates on the second survey on the sensitivity of isolates conducted by the japanese society of chemotherapy in 2007--concerning the aspect of pk/pd break points]. | sensitivity to beta-lactam antibiotics of isolates clinically obtained from respiratory infection sites in adults on the second survey on sensitivity of isolates conducted by the japanese society of chemotherapy in 2007 was investigated according to the classification of the "guideline for treatment for adult nosocomial pneumonia in 2008". among the primary antibacterial drugs for mild (a) and moderate (b) nosocomial pneumonia in adults, beta-lactam antibiotics; ceftriaxone (ctrx), sulbactam/amp ... | 2009 | 19882981 |
| uranium(vi) reduction and removal by high performing purified anaerobic cultures from mine soil. | biological uranium reduction was investigated using bacteria isolated from a uranium mine in limpopo, south africa. background uranium concentration in soil from the mine was determined to be 168 mgkg(-1) much higher than the typical background uranium concentration in natural soils (0.30-11.7 mgkg(-1)). therefore it was expected that the bacteria isolated from the site were resistant to u(vi) toxicity. preliminary studies using a non-purified consortium from the mine soil showed that u(vi) [ura ... | 2010 | 19883933 |
| molecular and biochemical characterization of the natural chromosome-encoded class a beta-lactamase from pseudomonas luteola. | pseudomonas luteola (formerly classified as cdc group ve-1 and named chryseomonas luteola) is an unusual pathogen implicated in rare but serious infections in humans. a novel beta-lactamase gene, bla(lut-1), was cloned from the whole-cell dna of the p. luteola clinical isolate lam, which had a weak narrow-spectrum beta-lactam-resistant phenotype, and expressed in escherichia coli. this gene encoded lut-1, a 296-amino-acid ambler class a beta-lactamase with a pi of 6 and a theoretical molecular m ... | 2010 | 19884377 |
| isolation and characterization of two members of the siderophore-microcin family, microcins m and h47. | in this paper we provide the first biochemical evidence of the existence of a family of structure-related antimicrobial peptides, the siderophore-microcins, in the enterobacteriaceae family. we isolated and characterized two novel siderophore-microcins, mccm and mcch47, previously characterized through genetic studies. mccm and mcch47 were expressed from several escherichia coli strains containing the microcin gene clusters. the spectra of their bactericidal activities were found to be restricte ... | 2010 | 19884380 |
| novel low-temperature-active phytase from erwinia carotovora var.carotovota accc 10276. | a phytase with high activity at low temperatures has great potential for feed applications, especially in aquaculture. therefore, this study used a degenerate pcr and tail pcr to clone a phytase gene from erwinia carotovora var. carotovota, the cause of soft rot of vegetables in the ground or during cold storage. the full-length 2.5-kb fragment included an open reading frame of 1,302 bp and encoded a putative phytase of 45.3 kda with a 50% amino acid identity to the klebsiella pneumoniae phytase ... | 2009 | 19884763 |
| interspecies spread of klebsiella pneumoniae carbapenemase gene in a single patient. | klebsiella pneumoniae carbapenemase (kpc)-producing k. pneumoniae, escherichia coli, and serratia marcescens were sequentially identified in a patient who underwent small bowel transplantation. molecular typing and plasmid analysis suggested that the kpc gene was acquired by e. coli, most likely from k. pneumoniae, and was subsequently transferred to s. marcescens. | 2009 | 19886795 |
| the spread of klebsiella pneumoniae carbapenemases: a tale of strains, plasmids, and transposons. | | 2009 | 19886796 |
| c-reactive protein and interleukin-6 responses for differentiating fungal and bacterial aetiology in late-onset neonatal sepsis. | fungal infections are increasingly frequent causes of neonatal sepsis (ns). this study examined the predictive value of the combined evaluation of the c-reactive protein (crp) and interleukin-6 (il-6) responses for differentiating fungal and bacterial aetiologies in patients with ns. from january to september 2007, neonates who were diagnosed with ns and had their crp and il-6 levels measured were selected. based on their blood culture results, the neonates were divided into two groups: group of ... | 2011 | 19889172 |
| kpc-2-producing klebsiella pneumoniae in brazil: a widespread threat in waiting? | | 2010 | 19889563 |
| [prevalence of extended-spectrum beta-lactamases of the ctx-m type producing escherichia coli and klebsiella pneumoniae in bretonneau hospitals (chru tours)]. | the ctx-m type extended spectrum beta-lactamases constitute a rapidly growing cluster of enzymes that have disseminated geographically. this study evaluates the prevalence of these enzymes in the university hospital in tours, in 2007. twenty-eight strains were studied: 21 escherichia coli and seven klebsiella pneumoniae. the gene bla(ctx-m) was detected by real-time pcr for 27 strains. the ctx-m-1 group, including ctx-m-15, was the most frequent ctx-m-type enzyme. | 2010 | 19892477 |
| authors' reply to: re: a vim-1 metallo-beta-lactamase-producing klebsiella pneumoniae clinical isolate in an acute hospital in germany. | | 2010 | 19892527 |
| comparison of 3 phenotypic-detection methods for identifying plasmid-mediated ampc beta-lactamase-producing escherichia coli, klebsiella pneumoniae, and proteus mirabilis strains. | plasmid-mediated ampc beta-lactamases (pabls) have been detected in the strains of escherichia coli, klebsiella spp., proteus mirabilis, and salmonella spp. pabls may be difficult to detect and might interfere in the therapeutic and infection-control processes. although several pabl-detection methods based on phenotypes have been reported, the clinical and laboratory standards institute currently does not recommend a routine detection method for pabls. the aim of this study is to compare the per ... | 2009 | 19893354 |
| in vitro activities of antimicrobials against six important species of gram-negative bacteria isolated from raw milk samples in korea. | gram-negative bacteria (gnb) with multidrug resistance pose a serious threat to public health. they are environmental pathogens frequently isolated from raw milk and mastitis in dairy cattle. this study was to examine the in vitro antimicrobial activities against 225 isolates belonging to six important species of gnb from mastitic raw milk samples of dairy herds in the republic of korea: acinetobacter baumannii (n = 17), citrobacter freundii (n = 19), enterobacter cloacae (n = 54), klebsiella pn ... | 2010 | 19895257 |
| antimicrobial substances produced by coliform strains active against foodborne pathogens. | in the present study, 31 coliform strains were isolated from salad, cheese, and meat products sold in commercial establishments in rio de janeiro city, and were tested for antibiotic resistance and antimicrobial substance production. thirteen strains (41.9%) were resistant to at least one antibiotic tested, among which one presented resistance to nine different antibiotics. two strains (6.4%) exhibited inhibitory activity against the indicator strains, escherichia coli lmifrj and salmonella ente ... | 2010 | 19895262 |
| chemical composition and antimicrobial and antioxidant activities of mentha (longifolia l. and viridis) essential oils. | the study was aimed to investigate essential oil chemical composition (gas chromatography/flame ionization detection [gc-fid] and gas chromatography/mass spectrometry [gc-ms]) and antioxidant (1,1-diphenyl-2-picrylhydrazyl free radical (dpph) and 2,2'-azinobis-3-ethylbenzothiazoline-6-sulphonate [abts] assays) and antimicrobial (gram-positive and gram-negative bacteria, fungi, and yeast) activities of essential oils extracted from leaves of mentha longifolia l. and mentha viridis. gc-ms analysis ... | 2009 | 19895481 |
| [ctx-m-15-producing klebsiella pneumoniae: a change in the epidemiology of esbl.] | klebsiella pneumoniae is frequently involved in nosocomial outbreaks worldwide. high level of resistance is common for these bacteria leading to reduce antibiotic treatments and prolonged hospital stay for patients. resistance determinants are often located on plasmids. during the 1980-1990s, esbl encoding genes belonged to the tem and shv type. from the early 2000s, a new trend was observed with esbl of the ctx-m type being increasingly described in k. pneumoniae, and more particularly ctx-m-15 ... | 2009 | 19896297 |
| joint extraintestinal manifestations in ulcerative colitis. | an extraintestinal manifestation (eim) very often occurs in ulcerative colitis (uc) patients. eim modifies the natural course of uc and decreases the quality of life in these patients. the aim of this study was to analyze clinical and laboratory findings in uc patients with joint eim. 319 uc patients were examined. among them were 131 (41.1%) patients with distal uc, 102 (32.0%) suffered from left-sided uc and 86 (26.9%) had pancolitis. 95 (29.8%) uc patients had joint eim. arthritis correlated ... | 2009 | 19897966 |
| usefulness of pcr melting profile method for genotyping analysis of klebsiella oxytoca isolates from patients of a single hospital unit. | the development of rapid and simple typing methods is required in order to identify possible sources of human exposure to opportunistic pathogens. klebsiella spp. belongs to a group of bacteria that are opportunistic pathogens responsible for an increasing number of multiresistant infections in hospitals. recently, we showed the high genetic diversity of k. oxytoca using a large collection of strains isolated from the patients of several hospitals in poland over a 50-year period. our results sho ... | 2009 | 19899618 |
| in vitro activity of fosfomycin against blakpc-containing klebsiella pneumoniae isolates, including those nonsusceptible to tigecycline and/or colistin. | in vitro activity of fosfomycin was evaluated against 68 bla(kpc)-possessing klebsiella pneumoniae (kpkpc) isolates, including 23 tigecycline- and/or colistin-nonsusceptible strains. by agar dilution, 93% of the overall kpkpc were susceptible (mic(50/90) of 16/64 microg/ml, respectively). the subgroup of 23 tigecycline- and/or colistin-nonsusceptible strains showed susceptibility rates of 87% (mic(50/90) of 32/128 microg/ml, respectively). notably, 5 out of 6 extremely drug-resistant (tigecyclin ... | 2010 | 19901089 |
| novel vim metallo-beta-lactamase variant from clinical isolates of enterobacteriaceae from algeria. | five different strains of bacteria belonging to the family enterobacteriaceae were isolated from two patients hospitalized in the intensive care unit of the central military hospital of algiers, algeria. all five strains, one providencia stuartii strain, two escherichia coli strains, and two klebsiella pneumoniae strains, were intermediate or resistant to all beta-lactams, including carbapenems. synergy between imipenem and edta was observed for all five strains. the results of the pcr experimen ... | 2010 | 19901092 |
| dispersal of carbapenemase blavim-1 gene associated with different tn402 variants, mercury transposons, and conjugative plasmids in enterobacteriaceae and pseudomonas aeruginosa. | the emergence of bla(vim-1) within four different genetic platforms from distinct enterobacteriaceae and pseudomonas aeruginosa isolates in an area with a low prevalence of metallo-beta-lactamase producers is reported. forty-three vim-1-producing isolates (including 19 enterobacter cloacae, 2 escherichia coli, and 2 p. aeruginosa isolates, 18 klebsiella pneumoniae isolate, and 2 klebsiella oxytoca isolate) recovered from 2005 to 2007 and corresponding to 15 pulsed-field gel electrophoresis types ... | 2010 | 19901094 |
| impact of a change in antibiotic prophylaxis on total antibiotic use in a surgical intensive care unit. | the aim of this study was to evaluate the impact of reducing the length of antibiotic prophylaxis for cerebro spinal shunts on total antibiotic use and key resistant pathogens. | 2010 | 19904488 |
| hospital and community isolates of uropathogens at a tertiary hospital in south africa. | to investigate the profile of common uropathogens isolated from urine specimens submitted to the diagnostic microbiology laboratory at a tertiary teaching hospital and assess their antimicrobial susceptibility patterns to commonly used antimicrobial agents. | 2009 | 19908617 |
| severe community-acquired acinetobacter baumannii pneumonia: an emerging highly lethal infectious disease in the asia-pacific. | patients with community-acquired acinetobacter baumannii (ab) pneumonia have been reported from subtropical countries. we investigated the epidemiology, clinical and microbiological characteristics of community-acquired pneumonia (cap) due to ab in singapore. | 2009 | 19909464 |
| pathogens resistant to antibacterial agents. | resistance to antimicrobial drugs is increasing at an alarming rate among both gram-positive and gram-negative bacteria. traditionally, bacteria resistant to multiple antimicrobial agents have been restricted to the nosocomial environment. a disturbing trend has been the recent emergence and spread of resistant pathogens in nursing homes, in the community, and in the hospital. this article reviews the epidemiology, molecular mechanisms of resistance, and treatment options for pathogens resistant ... | 2009 | 19909886 |
| infections and treatment of wounds in survivors of the 2004 tsunami in thailand. | on 26 december 2004, a tsunami devastated the west coast of thailand and caused 8457 injuries and 5395 deaths. data were collected from 26 december 2004 to 31 january 2005 at four public hospitals to describe the character and treatment of wounds of 523 persons who were injured during tsunami and sought medical treatment. wounds were contaminated with mud, sand, debris and sea water and had an infection rate of 66.5% (674/1013). most wounds (45%) had poly-microbial infection with gram-negative r ... | 2009 | 19912391 |
| a novel tetrabranched antimicrobial peptide that neutralizes bacterial lipopolysaccharide and prevents septic shock in vivo. | we describe the nonnatural antimicrobial peptide kkirvrlsa (m33) and its capacity to neutralize lps-induced cytokine release, preventing septic shock in animals infected with bacterial species of clinical interest. m33 showed strong resistance to proteolytic degradation when synthesized in tetrabranched form with 4 peptides linked by a lysine core, making it suitable for use in vivo. hplc and mass spectrometry demonstrated its stability in serum beyond 24 h. m33 was found to be very selective fo ... | 2010 | 19917670 |
| vim-19, a metallo-beta-lactamase with increased carbapenemase activity from escherichia coli and klebsiella pneumoniae. | two carbapenem-resistant isolates, one escherichia coli isolate and one klebsiella pneumoniae isolate, recovered from an algerian patient expressed a novel vim-type metallo-beta-lactamase (mbl). the identified bla(vim-19) gene was located on a ca. 160-kb plasmid and located inside a class 1 integron in both isolates. vim-19 differed from vim-1 by the asn215lys and ser228arg substitutions, increasing its hydrolytic activity toward carbapenems. site-directed mutagenesis experiments showed that bot ... | 2010 | 19917750 |
| automatic implantable cardioverter defibrillator pocket infection due to providencia rettgeri: a case report. | coagulase-negative staphylococci and staphylococcus aureus are the commonest pathogens involved in infections of pacemaker-defibrillator systems. among causative gram-negative bacteria, infections due to klebsiella, serratia, pseudomonas, acinetobacter and other species have been reported. we report herein a unique case of an automatic implantable cardioverter defibrillator infection due to providencia rettgeri in a 65-year-old male who was admitted to our service with bacteremia and infection o ... | 2009 | 19918391 |
| marked hydronephrosis and hydroureter after distigmine therapy in an adult male patient with paraplegia due to spinal cord injury: a case report. | distigmine, a long-acting anti-cholinesterase, is associated with side effects such as parkinsonism, cholinergic crisis, and rhabdomyolysis. we report a spinal cord injury patient, who developed marked hydronephrosis and hydroureter after distigmine therapy, which led to a series of complications over subsequent years. | 2009 | 19918519 |
| distribution and antimicrobial resistance of clinical and subclinical mastitis pathogens in dairy cows in rhône-alpes, france. | the goal of this study was to estimate the distribution of pathogens, as well as their antimicrobial resistance pattern, in cows affected by clinical or subclinical mastitis in the rhône-alpes region of france. a total of 1770 samples were taken between january 2007 and march 2008, leading to the identification of 1631 bacterial isolates. streptococcus uberis (22.1%), escherichia coli (16%), and coagulase-positive staphylococci (15.8%) were identified as the major causative agents of clinical ma ... | 2010 | 19919286 |
| proteomic analysis of the effect of cyanide on klebsiella oxytoca. | cyanide has been proved to be degraded by klebsiella oxytoca. in order to examine the physiological responses of cyanide degradation by this bacterium, two-dimensional (2-de) electrophoresis approach and maldi-tof-ms allow us to identify 106 proteins spots that were significantly altered in the presence of 1 mm cyanide in relative to that in 1 mm ammonia when k. oxytoca grown at the late-log phase. among them, 27 proteins were successfully identified. these proteins were involved in carbohydrate ... | 2010 | 19921330 |
| the phev phenylalanine trna gene klebsiella pneumoniae clinical isolates is an integration hotspot for possible niche-adaptation genomic islands. | horizontally acquired genomic islands may allow bacteria to conquer and colonize previously uncharted niches. four klebsiella pneumoniae trna gene insertion hotspots (arg6, asn34, met56, and phev) in 101 clinical isolates derived from blood, sputum, wound, bile or urine specimens were screened by long-range pcr for the presence or absence of integrated islands. the phev phenylalanine trna gene was the most frequently occupied site and harbored at least three entirely distinct types of islands: ( ... | 2010 | 19921332 |
| microbial fed-batch production of 1,3-propanediol using raw glycerol with suspended and immobilized klebsiella pneumoniae. | the production of 1,3-propanediol (1,3-pd) was investigated with klebsiella pneumoniae dsm 4799 using raw glycerol without purification obtained from a biodiesel production process. fed-batch cultures with suspended cells revealed that 1,3-pd production was more effective when utilizing raw glycerol than pure glycerol (productivity after 47 h of fermentation, 0.84 g l(-1) 1 h(-1) versus 1.51 g l(-1) h(-1) with pure and raw glycerol,respectively). in addition, more than 80 g/l of 1,3-pd was produ ... | 2010 | 19921491 |
| bacteraemia and candidaemia: a considerable and underestimated complication of severe rotavirus gastroenteritis. | despite the high incidence of rotavirus gastroenteritis, secondary bacteraemia later in the course of the disease has rarely been reported. to date, the exact incidence of this complication has not been determined. a prospective study was conducted between january 2007 and december 2008 to determine the incidence of bacteraemia by organisms of the normal intestinal flora during severe rotavirus gastroenteritis. rotavirus gastroenteritis was diagnosed by antigen detection in stool. a previously d ... | 2009 | 19922069 |
| expression of dha operon required for 1,3-pd formation in escherichia coli and saccharomyces cerevisiae. | the 1,3-propanediol (1,3-pd) synthesis operon (dha operon) was mainly composed of four genes: dhab, dhat, gdra, and gdrb, which encoded glycerol dehydratase, 1,3-pd oxidoreductase and reactivating factor for glycerol dehydratase, respectively. in the present study, dha operon was cloned from 1,3-pd producing strain klebsiella pneumoniae. heterologous expression of cloned dha operon was carried out in escherichia coli and saccharomyces cerevisiae w303-1a, respectively. the results indicated that ... | 2010 | 19924483 |
| beverages obtained from soda fountain machines in the u.s. contain microorganisms, including coliform bacteria. | ninety beverages of three types (sugar sodas, diet sodas and water) were obtained from 20 self-service and 10 personnel-dispensed soda fountains, analyzed for microbial contamination, and evaluated with respect to u.s. drinking water regulations. a follow-up study compared the concentration and composition of microbial populations in 27 beverages collected from 9 soda fountain machines in the morning as well as in the afternoon. ice dispensed from these machines was also examined for microbial c ... | 2010 | 19926155 |
| predictive factors for mortality and need for nephrectomy in patients with emphysematous pyelonephritis. | to analyse the factors predicting the mortality and need for nephrectomy in patients with emphysematous pyelonephritis (epn). | 2010 | 19930181 |
| multidetector row ct diagnosis of an infected right atrial thrombus following repeated dialysis catheter placement. | right atrial thrombus formation is a known complication of dialysis catheter placements. we describe the case of a 61-year-old woman with end-stage renal failure who presented with gram-negative septicaemia. a gas-containing filling defect was noted incidentally in the right atrium during a ct scan of the abdomen and pelvis, indicative of a thrombus infected by a gas-forming organism. the finding correlated with a positive blood culture of klebsiella pneumoniae and the two-dimensional echocardio ... | 2009 | 19934063 |
| [infectious endocarditis secondary to an infrequent agent in a patient with haemodialysis]. | | 2009 | 19936011 |
| extended spectrum beta-lactamases among gram-negative bacterial isolates from clinical specimens in three major hospitals in northern jordan. | background and objectives. extended spectrum beta-lactamase (esbl) production is increasing all over the world, and organisms other than e. coli and k. pneumoniae are acquiring this character. esbl production is detectable by automation, e-test, double disk diffusion (ddd), and pcr. this study aimed to determine the prevalence of esbl production among clinical isolates of gram-negative rods, and to evaluate the effectiveness of augmentation of clavunate with cefotaxime, ceftazoxime, aztreonam, c ... | 2009 | 19936109 |
| consequences of cps mutation of klebsiella pneumoniae on 1,3-propanediol fermentation. | the filtration in 1,3-propanediol (1,3-pd) downstream process is influenced by the large amounts of capsular polysaccharides (cps) produced by klebsiella pneumoniae cgmcc 1.6366. the morphological and fermentation properties were investigated with the cps-deficient mutant k. pneumoniae cgmcc 1.6366 cps. similar biomass was obtained with cgmcc 1.6366, and the mutant strain in batch cultures indicating the cell growth was slightly inhibited by cps defection. the viscosity of fermentation broth by ... | 2010 | 19936735 |
| effect of biodiesel-derived raw glycerol on 1,3-propanediol production by different microorganisms. | the microbial production of 1,3-propanediol (1,3-pd) from raw glycerol, a byproduct of biodiesel production, is economically and environmentally advantageous. although direct use of raw glycerol without any pretreatment is desirable, previous studies have reported that this could cause inhibition of microbial growth. in this study, we investigated the effects of raw glycerol type, different microorganisms, and pretreatment of raw glycerol on the production of 1,3-pd. raw glycerol from waste vege ... | 2010 | 19937397 |
| clinical significance of dysplastic squamous cells in exfoliative urine cytology. | | 2010 | 19937764 |
| spontaneous bacterial peritonitis: a severe complication of liver cirrhosis. | this report presents a survey of current knowledge concerning one of the relatively frequent and severe complications of liver cirrhosis and associated ascites-spontaneous bacterial peritonitis. epidemiology, aetiology, pathogenesis, clinical manifestation, diagnosis and present possibilities of treatment are discussed. | 2009 | 19938187 |
| [anaerobic non-clostridial phlegmon]. | | 2008 | 19938264 |
| plasmid pkpqil encoding kpc-3 and tem-1 confers carbapenem resistance in an extremely drug-resistant epidemic klebsiella pneumoniae strain. | an extremely drug-resistant (xdr) clone of kpc-3-producing klebsiella pneumoniae emerged in israel in 2006, causing a nationwide outbreak. we aimed to characterize the local kpc-3-encoding plasmid carried by these isolates and study its contribution to antibiotic resistance. | 2010 | 19939824 |
| role of yiax2 in l-ascorbate transport in klebsiella pneumoniae 13882. | the yiak-s operon is required for aerobic growth on l-ascorbate in several enterobacteriaceae. here we present evidence that the yiax2 gene belonging to the yiak-s operon of klebsiella pneumoniae 13882, which encodes a protein similar to the putative transporters classified as the major facilitator superfamily, is involved in the uptake of l-ascorbate. concentration kinetic analysis yielded an apparent k(m) of yiax2 for l-ascorbate of 161.38 +/-8.28 micromol x l(-1) and a vmax of 3.81 +/- 0.60 n ... | 2009 | 19940941 |
| epidemiology of bloodstream infections in patients with haematological malignancies with and without neutropenia. | all bacterial isolates from 7058 patients admitted to haemato-oncology wards at national taiwan university hospital between 2002 and 2006 were characterized. in total 1307 non-duplicate bloodstream isolates were made from all patients with haematological malignancy; 853 (65%) of these were from neutropenic patients. gram-negative bacteria predominated (60%) in neutropenic isolates with escherichia coli (12%), klebsiella pneumoniae (10%), acinetobacter calcoaceticus-baumannii complex (6%), and st ... | 2010 | 19941686 |
| in vitro synergistic effect of doxycycline & ofloxacin in combination with ethanolic leaf extract of vangueria spinosa against four pathogenic bacteria. | the in vitro antibacterial activity of ethanolic leaf extract of vangueria spinosa roxb. (rubiaceae) alone and in combination with antibiotics (doxycycline and ofloxacin) by means of fractional inhibitory concentration indices (fici) as well as by the use of time-kill assays against one gram-positive bacterium (staphylococcus aureus) and three gram-negative bacteria (escherichia coli, klebsiella pneumoniae and pseudomonas aeruginosa) was studied. | 2009 | 19942754 |
| [clinical aspects and epidemiology of uncomplicated cystitis in women. german results of the aresc study]. | uncomplicated cystitis in women is among the most frequent infections in the community setting. the german results of the international aresc study are reported concerning clinical aspects, epidemiology, and antimicrobial susceptibility of uropathogens. | 2010 | 19943031 |
| comparative study on characteristics of azo dye decolorization by indigenous decolorizers. | this study provides a first attempt to explore indigenous strains with excellent decolorization capability from the most biodiverse region in taiwan for dye-bearing wastewater treatment. bacterial isolates were obtained via serial selections under selection pressure of the fungicide nystatin and model textile dye(s). according to profiles of protein expression and pcr-augmented 16s rrna gene analyses for strain identification, >99% of nucleotide sequences in isolated strains were identical to ty ... | 2010 | 19944603 |
| extended-spectrum beta-lactamase-producing phenotype signifies a poor prognosis for patients with cefpodoxime-resistant escherichia coli or klebsiella pneumoniae bacteremia. | bloodstream infections caused by multidrug-resistant enterobacteriaceae are a major concern. this study explored the clinical impact of extended-spectrum beta-lactamase (esbl) production among cefpodoxime-resistant escherichia coli and klebsiella pneumoniae bacteremia. | 2009 | 19949753 |