| fed batch fermentation and purification strategy for high yield production of brucella melitensis recombinant omp 28 kda protein and its application in disease diagnosis. | brucellosis is a disease caused by bacteria belonging to the genus brucella. it affects cattle, goat, sheep, dog and humans. the serodiagnosis of brucellosis involves detection of antibodies generated against the lps or whole cell bacterial extracts, however these tests lack sensitivity and specificity. the present study was performed to optimize the culture condition for the production of recombinant brucella melitensis outer membrane protein 28 kda protein in e.coli via fed batch fermentation. ... | 2013 | 23167982 |
| pluronic p85 enhances the efficacy of outer membrane vesicles as a subunit vaccine against brucella melitensis challenge in mice. | brucellosis is the most common zoonotic disease worldwide, and there is no vaccine for human use. brucella melitensis rev1, a live attenuated strain, is the commercial vaccine for small ruminants to prevent b. melitensis infections but has been associated with abortions in animals. moreover, strain rev1 is known to cause disease in humans and cannot be used for human vaccination. outer membrane vesicles (omvs) obtained from b. melitensis have been shown to provide protection similar to strain re ... | 2012 | 23163875 |
| an atypical presentation of brucellosis in a patient with isolated thrombocytopenia complicated with upper gastrointestinal tract bleeding. | a 59-year-old female patient was admitted to the emergency service with complaints of hematemesis and melena for the last few days. in laboratory tests, the platelet count was found to be 6 × 10(9)/l. intravenous or oral corticosteroid treatment was thought to be given for itp but disclaimed due to upper gis bleeding. on the 5th day of treatment, brucella melitensis was isolated from blood culture before the results of wright tube agglutination tests were reported positive as 1 : 80. on the seco ... | 2012 | 23118764 |
| complete genome sequence of brucella melitensis biovar 3 strain ni, isolated from an aborted bovine fetus. | from an aborted bovine fetus in china, a bacterial strain named ni was isolated and identified as brucella melitensis by a pcr assay. strain ni was further characterized as b. melitensis biovar 3 using biochemical assays. here we report the complete genome sequence of strain ni. | 2012 | 23105063 |
| exact alpha-error determination for two-stage sampling strategies to substantiate freedom from disease. | sampling strategies to substantiate freedom from disease are important when it comes to the trade of animals and animal products. when considering imperfect tests and finite populations, sample size calculation can, however, be a challenging task. the generalized hypergeometric formula developed by cameron and baldock (1998a) offers a framework that can elegantly be extended to multi-stage sampling strategies, which are widely used to account for disease clustering at herd-level. the achieved al ... | 2013 | 23103193 |
| silencing of vamp3 expression does not affect brucella melitensis infection in mouse macrophages. | it has been proposed that intracellular pathogens may interfere with expression or function of proteins that mediate vesicular traffic in order to survive inside cells. brucella melitensis is an intracellular pathogen that evades phagosome-lysosome fusion, surviving in the so-called brucella-containing vacuoles (bcv). vesicle-associated membrane protein 3 (vamp3) is a v-snare protein that promotes the exocytosis of the proinflammatory cytokine tnf at the phagocytic cup when docking to its cognat ... | 2012 | 23076244 |
| genome sequences of three live attenuated vaccine strains of brucella species and implications for pathogenesis and differential diagnosis. | live attenuated vaccines play essential roles in the prevention of brucellosis. here, we report the draft genome sequences of three vaccine strains, brucella melitensis m5-10, b. suis s2-30, and b. abortus 104m. primary genome sequence analysis identified mutations, deletions, and insertions which have implications for attenuation and signatures for differential diagnosis. | 2012 | 23045513 |
| [brucella suis--a little-known zoonotic agent]. | brucellosis is a rare but serious bacterial zoonosis. officially, the czech republic is among countries that are free from brucellosis in livestock. in the country, sporadic imported human infections may occur, caused by e.g. brucella melitensis. in wild hare populations, however, rare cases of infection caused by brucella suis are still observed, potentially threatening humans. the short communication reports two cases of hare brucellosis in moravia, czech republic, and experiences with isolate ... | 2012 | 22997779 |
| human brucellosis outbreak acquired through camel milk ingestion in southern israel. | human brucellosis is common in southern israel among the semi-nomadic bedouin, a population that consumes unpasteurized dairy products. though camel milk ingestion is a known mechanism for brucellosis acquisition, only a few reports of sporadic cases have been published in the medical literature. | 2012 | 22977965 |
| genome sequences of brucella melitensis 16m and its two derivatives 16m1w and 16m13w, which evolved in vivo. | brucella melitensis is an intracellular pathogen that induces chronic infection in humans. here, we report the genome sequences of 16m and its two derivatives, 16m1w and 16m13w, which were allowed to adapt in vivo for 1 and 13 weeks, respectively. our findings contribute to the investigation of adaptive mutations and mechanisms of chronic infection by b. melitensis. | 2012 | 22965104 |
| genome sequence of brucella melitensis s66, an isolate of sequence type 8, prevalent in china. | brucella melitensis is the most-represented brucella species causing human brucellosis in china. here we report the complete genome sequence of b. melitensis strain s66, a representative strain of sequence type 8 (st8), which is prevalent in china, making it possible to compare the genome sequences of isolates from different countries. | 2012 | 22965081 |
| microrna expression profile in raw264.7 cells in response to brucella melitensis infection. | microrna (mirna) is small non-coding rna with approximate 22 nt in length. recent studies indicate that mirnas play significant roles in pathogen-host interactions. brucella organisms are gram-negative facultative intracellular bacteria that cause brucellosis. brucella strains infect macrophages and establish chronic infection by altering host life activities including apoptosis and autophagy. here, we report a comprehensive analysis of mirna expression profiles in mock- and brucella-infected ra ... | 2012 | 22904669 |
| an uncommon case of pediatric neurobrucellosis associated with intracranial hypertension. | we present the case of a 4-year-old boy who was admitted to hospital with intracranial hypertension, headache, diplopia, papilledema, and a normal brain mri. brucella melitensis in the cerebrospinal fluid was confirmed with pcr assay. we believe that neurobrucellosis should be included in the differential diagnosis when headaches persist following brucellosis. in addition, we suggest that when cerebrospinal fluid culture is negative, pcr may prove to be an optimal alternative tool for an immedia ... | 2012 | 22900217 |
| the two-component system prls/prlr of brucella melitensis is required for persistence in mice and appears to respond to ionic strength. | bacterial adaptation to environmental conditions is essential to ensure maximal fitness in the face of several stresses. in this context, two-component systems (tcss) represent a predominant signal transduction mechanism, allowing an appropriate response to be mounted when a stimulus is sensed. as facultative intracellular pathogens, brucella spp. face various environmental conditions, and an adequate response is required for a successful infection process. recently, bioinformatic analysis of br ... | 2012 | 22859617 |
| evaluation of the vitek 2 gram-negative (gn) microbial identification test card: collaborative study. | a collaborative study was conducted to evaluate the performance of the vitek 2 gram-negative (gn) identification card for use with the vitek 2 automated microbial identification system. the gn test card is used in the identification of fermenting and nonfermenting gram-negative bacilli, including the select agent organisms brucella melitensis, francisella tularensis, burkholderia mallei, b. pseudomallei, and yersinia pestis. the vitek 2 gn card is based on 47 biochemical tests measuring carbon s ... | 2012 | 22816270 |
| the pathophysiology of inhalational brucellosis in balb/c mice. | to characterize the clinical presentation and pathophysiology of inhalational brucellosis, balb/c mice were challenged with brucella melitensis 16m in a nose-only aerosol exposure chamber. a low dose of 1000 cfu/animal of b. melitensis resulted in 45% of mice with tissue burdens eight weeks post-challenge. the natural history of brucellosis in mice challenged by higher aerosol doses was examined by serial euthanizing mice over an eight week period. higher challenge doses of 1.00e+05 and 5.00e+05 ... | 2012 | 22773944 |
| inhibition of mcd14 inhibits tnfα secretion and no production in raw264.7 cells stimulated by brucella melitensis infection. | brucellosis, caused by brucella spp., is an important disease affecting not only human health, but also a number of animal species around the world. a receptor for lps of brucella and important innate immune molecule, cd14, has been implicated in the initiation of the inflammatory response to sepsis. evidence indicates that upstream inhibition of the lps initiated inflammatory pathway is an effective therapeutic approach for attenuating damaging immune activation. the aim of this study was to ex ... | 2012 | 22770519 |
| induction of protective immunity against brucellosis in mice by vaccination with a combination of naloxone, alum, and heat-killed brucella melitensis 16 m. | a t-helper cell type 1-specific response leads to the elimination of intracellular infection with brucella. studies have shown that naloxone (nlx) can promote a cellular immune response in this respect. the current study was carried out to evaluate the induction of protective immunity in mice against brucellosis by vaccination with a combination of nlx, alum, and heat-killed brucella melitensis 16 m (hkb). | 2013 | 22727892 |
| isolation of brucella melitensis biotype 3 from epidural empyema in a bosnian immigrant in germany. | brucellosis is a regionally emerging infectious disease in mediterranean countries with an increasing number of human cases and high morbidity rates. here, we describe a case of severe b. melitensis biotype 3 infection in an immigrant who had contact with ruminants during a short-term stay in bosnia before he returned to germany. the patient developed thoracic spondylodiscitis accompanied by a large epidural empyema and neurological deficits. the isolated strain was characterized and compared to ... | 2012 | 22683655 |
| molecular cloning of virb12 gene of brucella melitensis 16m strain in pet28a vector. | to clone the virb12 gene in pet28a expression vector for production of recombinant protein to be used as antigenic component for future serological test development. | 2012 | 22647810 |
| active evasion of ctl mediated killing and low quality responding cd8+ t cells contribute to persistence of brucellosis. | brucellosis is a common zoonotic disease that remains endemic in many parts of the world. dissecting the host immune response during this disease provides insight as to why brucellosis is often difficult to resolve. we used a brucella epitope specific in vivo killing assay to investigate the ability of cd8+ t cells to kill targets treated with purified pathogenic protein. importantly, we found the pathogenic protein tcpb to be a novel effector of adaptive immune evasion by inhibiting cd8+ t cell ... | 2012 | 22558103 |
| invasive escherichia coli vaccines expressing brucella melitensis outer membrane proteins 31 or 16 or periplasmic protein bp26 confer protection in mice challenged with b. melitensis. | because of the serious economic and medical consequences of brucellosis, efforts are to prevent infection of domestic animals through vaccines. many disadvantages are associated with the current brucella melitensis rev.1 vaccine prompting development of alternative vaccines and delivery. escherichia coli (dh5α) was engineered to express a plasmid containing the inv gene from yersinia pseudotuberculosis and the hly gene from listeria monocytogenes. these recombinant invasive e. coli expressing b. ... | 2012 | 22546330 |
| optimization and efficient purification of recombinant omp28 protein of brucella melitensis using triton x-100 and β-mercaptoethanol. | the high level expression of recombinant proteins in escherichia coli often leads to the formation of inclusion bodies that contain most of the expressed protein held together by non-covalent forces. the inclusion bodies are usually solubilized using strong denaturing agents like urea and guanidium hydrochloride. in this study recombinant omp28 (romp28) protein of brucella melitensis was expressed in two different vector systems and further efficient purification of the protein was done by modif ... | 2012 | 22542588 |
| purification, refolding and characterization of the trimeric omp2a outer membrane porin from brucella melitensis. | brucella melitensis is a gram-negative bacteria known to cause brucellosis and to produce severe infections in humans. whilst brucella's outer membrane proteins have been extensively studied due to their potential role as antigens or virulence factors, their function is still poorly understood at the structural level, as the 3d structure of brucella β-barrel membrane proteins are still unknown. in this context, the b. melitensis trimeric omp2a porin has been overexpressed and refolded in n-dodec ... | 2012 | 22538317 |
| otpr regulated the growth, cell morphology of b. melitensis and tolerance to β-lactam agents. | the intracellular pathogen, brucella melitensis, possesses an operon with two components: otpr (bmei0066), which encodes a response regulator, and bmei0067, which encodes a putative camp-dependent protein kinase regulatory subunit. previous studies have shown that a polar mutation in the bmei0066 gene significantly decreased virulence and stress tolerance in brucella. in this study, we constructed non-polar mutant with deletion of otpr, as well as its complementary strain to further investigate ... | 2012 | 22503392 |
| characterization of periplasmic protein bp26 epitopes of brucella melitensis reacting with murine monoclonal and sheep antibodies. | more than 35,000 new cases of human brucellosis were reported in 2010 by the chinese center for disease control and prevention. an attenuated b. melitensis vaccine m5-90 is currently used for vaccination of sheep and goats in china. in the study, a periplasmic protein bp26 from m5-90 was characterized for its epitope reactivity with mouse monoclonal and sheep antibodies. a total of 29 monoclonal antibodies (mabs) against recombinant bp26 (rbp26) were produced, which were tested for reactivity wi ... | 2012 | 22457830 |
| outbreak investigation of brucellosis in thassos, greece, 2008. | in spring 2008, the hellenic center for disease control and prevention was notified about human brucellosis cases in thassos, a greek island that had been up to that point under a brucellosis eradication programme. following the verification of the outbreak a 1:1 case– control study was conducted in the island. the study revealed that consumption of locally produced raw cheese was a risk factor for brucella melitensis infection (odds ratio (or): 15.1, 95% confidence interval (ci): 6.56–34.7). br ... | 2012 | 22449868 |
| brucellosis due to brucella suis in a swine herd associated with a human clinical case in the state of são paulo, brazil. | brucella suis has been recognized as the major etiological agent of human brucellosis in areas free from brucella melitensis infection. however, with changes in swine management, the occurrence of swine brucellosis has decreased as has the human incidence of b. suis infection. a swine brucellosis outbreak within a herd from jaboticabal (são paulo, brazil) was detected in july 2006. the herd comprised approximately 300 sows and 1,500 finishing animals. many sows within this herd experienced abort ... | 2012 | 22388711 |
| autophagy favors brucella melitensis survival in infected macrophages. | this study investigated the role of autophagy in the survival of the invasive bacterium brucella melitensis strain 16m in murine macrophages. here, brucella melitensis 16m was found to trigger autophagosome formation, enhance autophagy flux and increase the expression level of the autophagy marker protein lc3-ii. when autophagy was pharmacologically inhibited by 3-methyladenine (3-ma), brucella replication efficiency was significantly decreased (p < 0.05). these results suggest that autophagy fa ... | 2012 | 22367856 |
| vaccination programs for reproductive disorders of small ruminants. | vaccines are available for the control of contagious epididymitis and abortion in small ruminants, although many of them have significant limitations either in efficacy or safety to both the animals vaccinated and to the people handling the vaccine or animals. shelf-life of vaccines should be extended and improved, so that the vaccine remains effective with longer term storage and ideally without refrigeration, so that use in under-developed rural areas is not restricted (e.g., brucella melitens ... | 2012 | 22364910 |
| deep sequencing-based expression transcriptional profiling changes during brucella infection. | brucellosis is a worldwide zoonotic infectious disease that has significant economic effects on animal production and human health. the host macrophage -brucella interaction is critical to the establishment of infections. thus, the kinetic transcriptional profile of gene expression in macrophages infected with the brucella melitensis strain 16m was investigated in the current study using a technology based on deep sequencing. the total rna was extracted from macrophages 0, 4, and 24 h post-infec ... | 2012 | 22342430 |
| efficacy evaluation of live escherichia coli expression brucella p39 protein combined with cpg oligodeoxynucleotides vaccine against brucella melitensis 16m, in balb/c mice. | brucella is gram-negative bacteria responsible for brucellosis in a wide variety of animals and humans. balb/c mice were immunized with live escherichia coli expression the p39 gene of brucella melitensis, a gene coding for the periplasmic binding protein. mice were injected with either e. coli bl21 (de3) pet15b or e. coli bl21 (de3) pet15b-p39 alone or adjuvanted with either cpg oligodeoxynucleotides (cpg odn) or non-cpg odn. e. coli bl21 (de3) pet15b-p39 with cpg odn or with non-cpg odn mice g ... | 2012 | 22296786 |
| cross-border molecular tracing of brucellosis in europe. | to assess the general impact of endemic countries on the re-emergence of brucellosis in non-endemic regions of the european union, the genetic fingerprints of brucella melitensis strains imported to germany were compared to ovine strains from turkey in a molecular epidemiological study. genotyping of 66 brucella strains (based on multiple locus of variable number of tandem repeats analysis) isolated from german travellers and turkish immigrants living in germany revealed epidemiological concorda ... | 2012 | 22277829 |
| brucella endocarditis in prosthetic valves. | none declared. | 2012 | 24493988 |
| an immunosensing system using stilbene glycoside as a fluorogenic substrate for an enzymatic reaction model. | a natural product, stilbene glycoside (2,3,5,4'-tetrahydroxydiphenylethylene-2-o-glucoside, tbg), has been evaluated for the first time as a potential substrate for horseradish peroxidase (hrp)-catalyzed fluorogenic reactions. the properties of tbg as a fluorogenic substrate for hrp and its application in a fluorometric enzyme-linked immunosensing system were compared with commercially available substrates such as p-hydroxyphenylpropionic acid (phppa), chavicol and amplex red using brucella meli ... | 2008 | 27873835 |
| brucella melitensis dacryoadenitis: a case report. | to present a case of brucellosis-related unilateral dacryoadenitis. | 2017 | 28226144 |
| human to human transmission of brucella melitensis. | human brucellosis is acquired mainly through contact with infected animal tissues, ingestion of unpasteurized dairy products or infected aerosols. person to person transmission is still considered uncertain. the case of a woman diagnosed with proven brucellosis after her husband suffered a relapse of bacteremia with brucella melitensis biotype 3, which was originally acquired abroad by eating goat cheese, is described. it was postulated that person to person spread of brucellosis is a likely mod ... | 1995 | 22514390 |
| [neurobrucellosis: considerations in a case of meningoradiculitis from brucella melitensis: anatomo-pathologic aspects]. | | 1955 | 13303633 |
| preliminary report on the therapeutic action of methymycin in brucella melitensis infection. | | 1954 | 24542881 |
| mucosal bacterial dissemination in a rhesus macaque model of experimental brucellosis. | animals were experimentally infected with brucella melitensis via aerosol. b. melitensis was cultured from the saliva and vaginal vault of infected animals, corresponding to bacterial dissemination in other target tissues. this is the first report of bacterial dissemination to these mucosal surfaces in a non-human primate model of brucellosis. | 2017 | 28573738 |
| trypanosoma infection favors brucella elimination via il-12/ifnγ-dependent pathways. | this study develops an original co-infection model in mice using brucella melitensis, the most frequent cause of human brucellosis, and trypanosoma brucei, the agent of african trypanosomiasis. although the immunosuppressive effects of t. brucei in natural hosts and mice models are well established, we observed that the injection of t. brucei in mice chronically infected with b. melitensis induces a drastic reduction in the number of b. melitensis in the spleen, the main reservoir of the infecti ... | 2017 | 28824630 |
| using real-time polymerase chain reaction as an alternative rapid method for enumeration of colony count in live brucella vaccines. | brucellosis is a major bacterial zoonosis of global importance affecting a range of animal species and man worldwide. it has economic, public health, and bio-risk importance. control and prevention of animal brucellosis mainly depend on accurate diagnostic tools and implementation of effective and safe animal vaccination program. there are three types of animal brucella live vaccines - brucella melitensis rev-1 vaccine, brucella abortus s19, and b. abortus rb51. evaluation of these vaccines depe ... | 2017 | 28717311 |
| brucella infection in asian sea otters (enhydra lutris lutris) on bering island, russia. | infection with brucella spp., long known as a cause of abortion, infertility, and reproductive loss in domestic livestock, has increasingly been documented in marine mammals over the past two decades. we report molecular evidence of brucella infection in asian sea otters (enhydra lutris lutris). brucella dna was detected in 3 of 78 (4%) rectal swab samples collected between 2004 and 2006 on bering island, russia. these 78 animals had previously been documented to have a brucella seroprevalence o ... | 2017 | 28715292 |
| evaluation of plasma sphingosine 1-phosphate, hepcidin and cardiovascular damage biomarkers (cardiac troponin i and homocysteine) in rats infected with brucellosis and vaccinated (rev-1, rb-51). | brucellosis is known as one of important zoonosis. studying the histological and biochemical effects of the disease could help to increase our knowledge about it. the aim of the present study was to evaluate changes of plasma parameters after intraperitoneal injection of two species of brucella (brucella melitensis and brucella abortus) and two vaccines (rev-1, rb-51) in the rat. forty male rats were divided into five groups (n = 8 in each group). two groups received suspensions of brucella abor ... | 2017 | 28533142 |
| molecular investigation of virulence factors of brucella melitensis and brucella abortus strains isolated from clinical and non-clinical samples. | brucella is zoonotic pathogen that induces abortion and sterility in domestic mammals and chronic infections in humans called malta fever. it is a facultative intracellular potential pathogen with high infectivity. the virulence of brucella is dependent upon its potential virulence factors such as enzymes and cell envelope associated virulence genes. the aim of this study was to investigate the brucella virulence factors among strains isolated from humans and animals in different parts of iran. ... | 2017 | 28506887 |
| a novel pcr assay for detecting brucella abortus and brucella melitensis. | brucellosis is a major zoonotic disease that poses a significant public health threat worldwide. the classical bacteriological detection process used to identify brucella spp. is difficult and time-consuming. this study aimed to develop a novel molecular assay for detecting brucellosis. | 2017 | 28443226 |
| protein/protein, dna/dna and dna/protein based vaccination strategies using truncated omp2b against brucella infection in balb/c mice. | the purpose of the present study was to evaluate the immunogenicity and protective efficacy of the truncated form of outer membrane protein 2b (tomp2b) from brucella abortus in balb/c mice. three immunization regimens protein/protein, dna/dna and dna/protein were used. immunization of mice with all vaccine strategies elicited a strong specific igg responses (igg2a titers over igg1) and provided t helper1 (th1) oriented immune responses. furthermore, protein/protein (pro/pro-) and dna/pro- vaccin ... | 2017 | 28391992 |
| whole-genome sequences of brucella melitensis strain qy1, isolated from sheep in gansu, china. | the facultative intracellular gram-negative bacterium brucella melitensis causes brucellosis in domestic and wild mammals, and it is a dominant pathogen responsible for human disease. this study reports the whole-genome sequencing of b. melitensis strain qy1, isolated from sheep suffering from abortion and arthritis in 2015 in gansu, china. | 2017 | 28860252 |
| caprine brucellosis: a historically neglected disease with significant impact on public health. | caprine brucellosis is a chronic infectious disease caused by the gram-negative cocci-bacillus brucella melitensis. middle- to late-term abortion, stillbirths, and the delivery of weak offspring are the characteristic clinical signs of the disease that is associated with an extensive negative impact in a flock's productivity. b. melitensis is also the most virulent brucella species for humans, responsible for a severely debilitating and disabling illness that results in high morbidity with inter ... | 2017 | 28817647 |
| systems biology analysis of temporal in vivo brucella melitensis and bovine transcriptomes predicts host:pathogen protein-protein interactions. | to date, fewer than 200 gene-products have been identified as brucella virulence factors, and most were characterized individually without considering how they are temporally and coordinately expressed or secreted during the infection process. here, we describe and analyze the in vivo temporal transcriptional profile of brucella melitensis during the initial 4 h interaction with cattle. pathway analysis revealed an activation of the "two component system" providing evidence that the in vivo bruc ... | 2017 | 28798726 |
| genome sequences of brucella melitensis, isolated from blood samples of brucellosis patients in malaysia. | human brucellosis is a neglected zoonotic disease and has widespread geographical distribution. brucella melitensis has caused outbreaks and sporadic cases in malaysia. here, we present the whole-genome sequences of four b. melitensis strains isolated from brucellosis patients in malaysia. | 2017 | 28774972 |
| [hoof genotyping characteristics of brucella melitensis strains isolated in ulanqab of inner mongolia autonomous region]. | objective: to investigate the hoof genotyping characteristics of 83 brucella (b.) melitensis strains isolated in ulanqab of inner mongolia autonomous region from 2012 to 2015. methods: a total of 83 b. melitensis strains were detected by convention identification and amos-pcr, then hoof protocol with eight vntr locus were used for the genotyping of the strains, and the allelic diversity of each vntr locus and the discriminatory power of vntr typing of hoof were assessed by hunter-gaston discrimi ... | 2017 | 28738473 |
| comparative study of immunopathophysiological responses induced by b. melitensis and its lipopolysaccharide in mouse model infected via intranasal route of exposure. | in this study, we developed a mouse model and characterized the effects of intranasal inoculation of virulent brucella melitensis strain 16m and its lipopolysaccharide (lps). the effects of the exposure were compared with respective control groups. both brucella melitensis-infected and lps-infected groups showed no significant clinical presentation with minor relevance in the mortality associated with the infection. in brucella melitensis-infected group, significant histopathological changes in ... | 2017 | 28710016 |
| brucella pericarditis: a forgotten cause of chest pain. | brucellosis pericarditis is an extremely rare involvement of the cardiovascular system with brucella species that has worldwide distribution and is endemic in many provinces of iran. | 2017 | 28702152 |
| in vitro synergistic effects of a short cationic peptide and clinically used antibiotics against drug-resistant isolates of brucella melitensis. | in the last few decades, increasing microbial resistance to common antibiotics has attracted researchers' attention to the development of new classes of antibiotics such as antimicrobial peptides. accordingly, the aim of the current study was to evaluate antimicrobial effects of the cm11 peptide alone and combined with common antibiotics against drug-resistant isolates of brucella melitensis. | 2017 | 28699872 |
| human brucella melitensis infections in southern vietnam. | | 2017 | 28669842 |
| comparative diagnostic evaluation of omp31 gene based taqman® real-time pcr assay with visual lamp assay and indirect elisa for caprine brucellosis. | brucellosis is one of the leading causes of abortion in domestic animals that imposes costs on both economy and society. the disease is highly zoonotic and poses risk to animal handlers due to its zoonotic nature. it causes stillbirth, loss of kids and abortion in last term of pregnancy. reproductive damage includes infertility in does and orchitis and epididymitis in breeding bucks, which result in high financial losses to farmers and the agriculture industry as a whole. it requires highly sens ... | 2017 | 28638960 |
| biochemical characterization of wbkc, an n-formyltransferase from brucella melitensis. | it has become increasingly apparent within the last several years that unusual n-formylated sugars are often found on the o-antigens of such gram negative pathogenic organisms as francisella tularensis, campylobacter jejuni, and providencia alcalifaciens, among others. indeed, in some species of brucella, for example, the o-antigen contains 1,2-linked 4-formamido-4,6-dideoxy-α-d-mannosyl groups. these sugars, often referred to as n-formylperosamine, are synthesized in pathways initiating with gd ... | 2017 | 28636341 |
| endogenous brucella endophthalmitis: a case report. | brucellosis may be associated with a wide range of ophthalmic manifestations including endophthalmitis, which is a sight-threatening condition that needs to be rapidly recognized and treated to avoid permanent visual loss. a 26-year-old female with a 6-month history of vision loss in the left eye was treated with high dose systemic corticosteroids and azathioprine with an initial misdiagnosis elsewhere. a dense vitreous haze with opacities at the posterior hyaloid and a wide area of retinochoroi ... | 2017 | 28559723 |
| brucella melitensis prosthetic joint infection. | periprosthetic joint infection (pji) due to brucella spp. is rare. we report a case in a 75-year-old man and review 29 additional cases identified in a literature search. the diagnosis of brucella pji is challenging, in particular in non-endemic countries. serological tests prior to joint aspiration or surgical intervention are reasonable. involvement of infection control and timely information to laboratory personnel is mandatory upon diagnosis. there is no uniform treatment concept, neither wi ... | 2017 | 28540150 |
| transcriptional control of the lateral-flagellar genes of bradyrhizobium diazoefficiens. | bradyrhizobium diazoefficiens, a soybean n2-fixing symbiont, possesses a dual flagellar system comprising a constitutive subpolar flagellum and inducible lateral flagella. here, we analyzed the genomic organization and biosynthetic regulation of the lateral-flagellar genes. we found that these genes are located in a single genomic cluster, organized in two monocistronic transcriptional units and three operons, one possibly containing an internal transcription start site. among the monocistronic ... | 2017 | 28533217 |
| characterization of novel omp31 antigenic epitopes of brucella melitensis by monoclonal antibodies. | brucellosis is a severe zoonotic disease worldwide. detection and identification of brucella species are essential to prevent or treat brucellosis in humans and animals. the outer membrane protein-31 (omp31) is a major protein of brucellae except for b. abortus, while the omp31 antigenic epitopes have not been extensively characterized yet. | 2017 | 28506316 |
| brucella melitensis m5-90δbp26 as a potential live vaccine that allows for the distinction between natural infection and immunization. | brucella is gram-negative intracellular bacterial pathogen that infects humans and animals and contributes to great economic losses in developing countries. presently, live attenuated brucella vaccines (brucella melitensis m5-90) are the most effective means of brucellosis control and prevention in animals. however, these vaccines have several drawbacks, such as an inability to distinguish between a natural infection and immunization and an association with abortions in pregnant animals. therefo ... | 2017 | 28482164 |
| a case report of pediatric brucellosis in an algerian immigrant. | an 8-year old girl presented to our facility with a 10-day history of fever, fatigue, abdominal pain and refusal to walk. she recently travelled from her native algeria where she first developed symptoms. on evaluation, she was ill-appearing, febrile and tachycardic with hepatosplenomegaly and lymphadenopathy noted on examination. a strong musty odor was also noted from the child. laboratory evaluation revealed pancytopenia, hyponatremia, and an elevated ast, alt, and ldh. malaria testing was ne ... | 2017 | 28480256 |
| high throughput virtual screening to identify novel natural product inhibitors for methionyltrna-synthetase of brucella melitensis. | the brucella melitensis methionyl-trna-synthetase (metrsbm) is a promising target for brucellosis drug development. the virtual screening of large libraries of a drug like molecules against a protein target is a common strategy used to identify novel inhibitors. a high throughput virtual screening was performed to identify hits to the potential antibrucellosis drug target, metrsbm. the best inhibitor identified from the literature survey was 1312, 1415, and 1430. in the virtual screening 56,400 ... | 2017 | 28479744 |
| cd14 gene silencing alters the microrna expression profile of raw264.7 cells stimulated by brucella melitensis infection. | innate recognition of brucella spp. is a key step in the activation of inflammation. cd14 binds pamps and is involved in lps-induced pro-inflammatory cytokine release. previously we showed that knock down of cd14 in raw264.7 macrophages disrupted brucella-host interactions. however, its effect on the macrophage microrna (mirna) expression profile, especially after stimulation by brucella infection, is still unclear. to identify mirnas involved in the macrophage response to brucella infection, we ... | 2017 | 28443393 |