| parvovirus b19-related anaemia after renal transplantation. | we describe here the case of a renal transplant recipient treated by sirolimus based immunosuppresive therapy, who developed severe and unusual pancytopenia 2 months after renal transplantation. parvovirus b19 primo-infection was diagnosed. the first course of intravenous immunoglobulin failed. bone marrow aspiration confirmed megaloblastic anaemia associated with parvovirus b19. finally, this infection was succesfully treated by the reduction of immunosuppression combined with a second course o ... | 2004 | 15307588 |
| t-helper cell-mediated interferon-gamma, interleukin-10 and proliferation responses to a candidate recombinant vaccine for human parvovirus b19. | recombinantly expressed virus-like particles of human parvovirus b19 containing the two structural proteins vp1 and vp2 (vp1/2 capsids) or vp2 alone (vp2 capsids) elicit vigorous antibody responses in animal models, whereas only vp1/2 capsids elicit neutralizing antibodies. vp1 is, therefore, essential for protective b-cell immunity. in this study, we determined the ability of vp1/2 capsids containing vp1 and vp2 in the ratio recommended for vaccine use, and of sole vp2 capsids to stimulate t-he ... | 2004 | 15315862 |
| [viral exanthem]. | the most frequent cause of exanthematous diseases are viral infections, which provoke skin alterations either directly or via the reaction of the immune system. in many distinct parainfectious clinical pictures, several viruses from quite different groups are able to produce a specific exanthem. dominant pathogens for exanthematous diseases include non-polio enteroviruses, respiratory viruses, epstein-barr virus, hhv-6 and hhv-7 viruses as well as parvovirus b19. in many cases exanthemata presen ... | 2004 | 15316636 |
| an assay for parvovirus b19 neutralizing antibodies based on human hepatocarcinoma cell lines. | although intravenous immune globulin (ivig) is used widely for managing parvovirus b19 infections, ivig products are not monitored routinely for the presence of parvovirus b19 neutralizing antibody. | 2004 | 15318858 |
| the relationship between arthritis and human parvovirus b19 infection. | in order to evaluate the role of human parvovirus b19 in the etiopathogenesis of autoimmune diseases such as rheumatoid arthritis (ra) and systemic lupus erythematosus (sle), synovial fluid and blood specimens were collected at 1-month intervals from 20 patients with early synovitis (es) and 31 with ra. blood specimens were also collected from 25 patients with sle, 25 with osteoarthritis (oa) as the diseased control group, and 50 healthy blood donors (hbd) as the healthy control group. detection ... | 2005 | 15322815 |
| familiar occurrence of papular-purpuric 'gloves and socks' syndrome with human herpes virus-7 and human parvovirus b19 infection. | | 2004 | 15324419 |
| hypoxia enhances human b19 erythrovirus gene expression in primary erythroid cells. | human b19 erythrovirus replicates in erythroid progenitors present in bone marrow and fetal tissues where partial oxygen tension is low. here we show that infected human primary erythroid progenitor cells exposed to hypoxia (1% o2) in vitro increase viral capsid protein synthesis, virus replication, and virus production. hypoxia-inducible factor-1 (hif-1), the main transcription factor involved in the cellular response to reduced oxygenation, is shown to bind an hif binding site (hbs) located in ... | 2004 | 15327892 |
| molecular testing for detection of in vitro infectivity of plasma pools contaminated with b19 virus. | b19 virus can be transmitted by contaminated blood or blood products. recent observations, in healthy volunteers, suggest that active b19 infection can follow the administration of plasma pools with a concentration > or =10(7) genome equivalents/ml (geq/ml) of b19 dna. however, patients receiving batches with levels of virus dna lower than 10(4) geq/ml do not show any evidence of transmission of the virus. the aim of the study was to show, by in vitro assays, a threshold of viral load in b19 con ... | 2004 | 15332276 |
| ulcerative colitis that developed 8 years after human parvovirus b19 encephalopathy. | | 2004 | 15346923 |
| evaluation of different assays for the detection of parvovirus b19 dna in human plasma. | human parvovirus b19 is a frequent contaminant of blood and plasma-derived medicinal products and transmission of this virus has been shown to occur through the administration of contaminated products. inactivation of the virus has proved difficult and as a consequence, manufacturers of blood products have implemented screening measures to reduce the load of parvovirus b19 in manufacturing plasma pools by the use of nucleic acid amplification techniques (nat). we present data comparing the perfo ... | 2004 | 15350727 |
| cytomegalovirus prevalence and transmission after islet allograft transplant in patients with type 1 diabetes mellitus. | cytomegalovirus (cmv) serological status of transplant donors and recipients has important implications on antiviral prophylaxis, morbidity/mortality, donor selection and hospital stay. we evaluated cmv prevalence in our islet transplant candidates (itc) in comparison with organ donors. we correlated the cmv serological status of our itc with serology for epstein-barr virus and parvovirus b19, auto-antibodies, patient's age, age at dm onset, duration of dm, gender, race, abo group, hla haplotype ... | 2004 | 15367227 |
| human parvovirus b19 non-structural protein (ns1) induces apoptosis through mitochondria cell death pathway in cos-7 cells. | human parvovirus b19 has been found in various tissues in addition to erythroid lineage cells, and non-structural protein (ns1) is reported to induce cytotoxicity and apoptosis in erythroid lineage cells, but the mechanism in non-permissive cells is still unclear. to address this issue, we have constructed the ns1 gene in a cytomegalovirus episomal vector, pegfp-c1 and transfected it into monkey epithelial cells, cos-7. egfp-ns1 expression in transfected cells was monitored and assessed by fluor ... | 2004 | 15370668 |
| hypoxia enables b19 erythrovirus to yield abundant infectious progeny in a pluripotent erythroid cell line. | b19 may cause mild to severe clinical manifestations. owing to the remarkable tropism of b19 for red blood cell progenitors, there is a lack of satisfactory cell lines fully permissive for b19. because the local oxygen pressure may influence viral replication, we used hypoxia to improve the sensitivity of our infectivity assay in order to link b19 dna detected by pcr to the presence of infectious b19 particles in plasma. plasma samples and the who international standard for b19 dna detection by ... | 2004 | 15381351 |
| human parvovirus b19 transgenic mice become susceptible to polyarthritis. | human parvovirus b19 (b19) often causes acute polyarthritis in adults. in this paper, we analyzed nucleotide sequences of the b19 genome of patients with rheumatoid arthritis (ra), and then introduced the nonstructural protein 1 (ns1) gene of b19 into c57bl/6 mice that had a genetic origin not susceptible to arthritis. the transgenic mice developed no lesions spontaneously, but were susceptible to type ii collagen (cii)-induced arthritis. b19 ns1 was expressed in synovial cells on the articular ... | 2004 | 15383603 |
| splice junction map of simian parvovirus transcripts. | the transcription map of simian parvovirus (spv), an erythrovirus similar to parvovirus b19, was investigated. rna was extracted from tissues of experimentally infected cynomolgus macaques and subjected to reverse transcription-pcr with spv-specific primers. the pcr products were cloned and sequenced to identify splice junctions. a total of 14 distinct sequences were identified as putative partial transcripts. of these, 13 were spliced; a single unspliced transcript putatively encoded ns1. seque ... | 2004 | 15452211 |
| parvovirus b19 infection presenting as pre-b-cell acute lymphoblastic leukemia: a transient and progressive course in two children. | parvovirus b19 is the causative agent of various forms of hematologic diseases such as aplastic crisis in patients with hemolytic anemia, aplastic anemia, hypoplastic anemia, and idiopathic thrombocytopenic purpura. in addition, parvovirus b19 infection may precede or be associated with acute lymphoblastic leukemia (all). the authors present two cases of parvovirus b19 infection and bone marrow infiltration with pre-b-cell lymphoblasts; one patients was diagnosed as having all, and the other pat ... | 2004 | 15454845 |
| [endomyocardial biopsy-guided diagnosis and treatment of inflammatory cardiomyopathies]. | over 50 % of cases of inflammatory cardiomyopathy are caused by bacterial or viral infection, the latter frequently parvovirus b19, enterovirus (coxsackie b virus) or adenovirus. regarding the pathogenesis of the disease, its early phase is dominated by the infectious pathogen, which directly damages the myocardium, while in the second phase an important role is played by activation of the immune system and the antiviral immune response with immunological processes. | 2004 | 15457396 |
| [virus etiology of inflammatory cardiomyopathy]. | molecular biological methods such as in situ hybridization and the polymerase chain reaction (pcr) have confirmed the pathogenetic role of enteroviruses and primarily coxsackieviruses of group b (cvb) in the induction and maintenance of inflammatory cardiomyopathy. more recently, additional viruses such as adenoviruses (adv), various herpes viruses and increasingly parvovirus b19 (pvb19) have been identified as potential cardiotropic agents in the human heart. the different cell tropism of cardi ... | 2004 | 15457399 |
| [parvovirus b19 infection--dangerous for the fetus]. | human parvovirus b19 infection is a common and usually benign disease in children, but may cause severe complications in pregnancy. we determined the proportion of the pregnant population at risk of infection and present a recent cluster of cases with fetal complications, hoping to raise the general awareness. | 2004 | 15467798 |
| contamination of coagulation factor concentrates with human parvovirus b19 genotype 1 and 2. | human parvovirus b19 (b19) dna has frequently been detected in plasma-derived coagulation factor concentrates. furthermore, transmission of b19 infection was observed, indicating presence of the infectious virus despite routine viral inactivation/removal procedures during the manufacturing process. recently, human parvovirus dna isolates, variant from b19, have been identified resulting in classification of b19 virus into three distinct genotypes, with all viruses previously classified as b19 be ... | 2004 | 15467916 |
| persistence of parvovirus b19 igm antibodies and dna in pure red cell aplasia resulting in myelodysplasia--a case report. | human erythrovirus b19 (b19), previously known as parvovirus b19, is a small spherical, non-enveloped single stranded dna virus. it has been shown to cause a wide spectrum of clinical conditons including various hematological disorders. we report here for the first time from inida a case of pure red cell aplasia in a 45-year-old female for last 7 years due to chronic persistent b19 infection leading to myelodysplasia after 4 years. her sera were positive for two times 4 months apart for b19 igm ... | 2004 | 15471143 |
| [systemic manifestations of parvovirus b19 infections]. | purpose: parvovirus b19 (b19) causes many clinical disorders, of which the most common are erythema infectiosum, aplastic crisis complicating chronic hemolytic anemia, and hydrops fetalis. in young adults, the skin eruption caused by b19 is accompanied by polyarthritis and polyarthralgia in 60% of the cases. rheumatoid factors and other antibodies including antinuclear antibodies, anti-adn, and antiphospholipids can be produced in the wake of b19 infection. current knowledge and key points: thes ... | 2004 | 15471600 |
| seroprevalence of igg against conformational and linear capsid antigens of parvovirus b19 in italian blood donors. | serum samples from 446 italian blood donors between 18 and 65 years of age were analysed for the presence of igg against parvovirus b19 capsid proteins vp1 and vp2 including conformational and linear epitopes. the overall prevalence of igg against parvovirus b19 capsid proteins vp1 and vp2 against at least one antigen type was 79.1 %. no significant difference was found between men and women. in the 18-27 years age group, 77.0 % of the population had experienced infection with the virus, reachin ... | 2004 | 15473148 |
| the canine parvovirus wild-type strains infections in dogs--epidemiological and diagnostic aspects. | biological material was taken from dogs with diarrhea. faecal samples were taken from live animals white intestinal tract fragments (i.e. small intestine, and stomach) were taken from dead animals. in total, 18 specimens were investigated from dogs housed alone or in large groups. the samples were examined for presence of viral infections and concurrent bacterial and parasitic infestations. to test for the presence of the viral infection, latex (on site biotech, sweden) and direct immunofluoresc ... | 2004 | 15478865 |
| parvovirus b19 infections. | abstract parvovirus b19 infections cause a wide range of clinical findings, including erythema infectiosum, "glove-and-socks" syndrome, arthropathies, red cell aplasia, and intrauterine infections. the condition can be diagnosed serologically. the prognosis is excellent in healthy, immunocompetent individuals. | 2004 | 15485533 |
| clinical interpretation of antineutrophil cytoplasmic antibodies: parvovirus b19 infection as a pitfall. | while antibodies directed against proteinase 3 (pr3-anca) and myeloperoxidase (mpo-anca) have a high specificity for the diagnosis of systemic vasculitis, they may also be found as an epiphenomenon of acute viral infection. | 2005 | 15485998 |
| interstitial lung disease with cytomegalovirus and parvovirus b19 coinfection. | | 2004 | 15487462 |
| molecular diagnostics in virology. | molecular biology has significantly improved diagnosis in the field of clinical virology. virus discovery and rapid implementation of diagnostic tests for newly discovered viruses has strongly beneficiated from the development of molecular techniques. viral load and antiviral resistance or subtyping assays are now part of the biological monitoring of patients chronically infected by human immunodeficiency virus (hiv), hepatitis b virus (hbv), hepatitis c virus (hcv) and cmv. it will be important ... | 2004 | 15494263 |
| [secondary nephrotic syndrome induced by infection]. | infections can be the causative agent in secondary nephrotic syndrome and diagnostic criteria include clinical and laboratory data and tissue molecular analysis. as for bacterial infections, some of patients with poststreptococcal glomerulonephritis or methicillin-resistant staphylococcus aureus-related glomerulonephritis present nephrotic syndrome. hepatitis b virus (hbv) is a well known cause of membranous glomerulonephritis and membranoproliferative glomerulonephritis. hepatitis c virus (hcv) ... | 2004 | 15500141 |
| [what have we learned about giant cell arteritis during the last decade?]. | introduction: we propose a review on the current state of knowledge in giant cell arteritis (gca), with particular emphasis on advances produced during the past 10 years. current knowledge and key points: the physiopathology of gca is better understood. gca is considered as a t-cell mediated, antigen driven disease. the expression of interferon-gamma greatly influences the clinical expression of the vasculitis. the disease occurs preferentially in women. hladr4 group and an atheromatous backgrou ... | 2004 | 15501351 |
| human parvovirus b19. | | 2004 | 15504221 |
| endothelium-dependent flow-mediated vasodilation of systemic arteries is impaired in patients with myocardial virus persistence. | myocardial virus persistence is frequently observed in patients with cardiomyopathy. endothelial dysfunction in patients with cardiomyopathy is associated with inflammatory immunoresponses in myocardial biopsies. the aim of this study was to investigate the impact of myocardial virus persistence on endothelial function. | 2004 | 15505098 |
| identification and characterization of persistent human erythrovirus infection in blood donor samples. | the presence of human erythrovirus dna in 2,440 blood donations from the united kingdom and sub-saharan africa (ghana, malawi, and south africa) was screened. sensitive qualitative and real-time quantitative pcr assays revealed a higher prevalence of persistent infection with the simultaneous presence of immunoglobulin g (igg) and viral dna (0.55 to 1.3%) than previously reported. this condition was characterized by a low viral load (median, 558 iu/ml; range, 42 to 135,000 iu/ml), antibody-compl ... | 2004 | 15507603 |
| early presentation and spontaneous resolution of hydrops fetalis, secondary to parvovirus b19 infection. | | 1998 | 15512050 |
| human parvovirus b19 nonstructural protein transactivates the p21/waf1 through sp1. | the expression of human parvovirus b19 nonstructural protein 1 (ns1) induces cell cycle arrest at the g1 phase and is accompanied by increased expression of the cyclin-dependent kinase inhibitor, p21/waf1. here, we provide direct evidence that ns1 mediates the transactivation of p21/waf1. up-regulation of p21/waf1 by wild-type ns1 but not an ns1 mutant deleted of its ntp binding motif was observed. we also demonstrated that the wild-type ns1 is unable to induce g1 arrest in p21-deficient cells. ... | 2004 | 15518826 |
| differential transmission of parvovirus b19 in a twin gestation: a case report. | maternal infection with parvovirus b19 during pregnancy can cause aplastic anemia in the fetus. severe anemia may lead to nonimmune hydrops or fetal demise. in the case reported, the demise of one twin was diagnosed by ultrasonography in an asymptomatic 21-year-old para 1-0-2-1 african american at the gestational age of 25 weeks. the deceased twin (a) was grossly hydropic with anasarca, ascites, pleural and pericardial effusions, and a thickened placenta. parvovirus b19 dna was found in the amni ... | 2004 | 15527655 |
| real-time quantitative pcr assays for detection and monitoring of pathogenic human viruses in immunosuppressed pediatric patients. | a panel of 23 real-time pcr assays based on taqman technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses bk virus and jc virus, human herpesviruses 6, 7, and 8, human adenoviruses, herpes simplex viruses 1 and 2, varicella-zoster virus, cytomegalovirus, epstein-barr virus, parvovirus b19, influenza a and b viruses, parainfluenza viruses 1 to 3, enteroviruses, and respiratory syncytial virus. the test systems prese ... | 2004 | 15528714 |
| simian parvovirus infection: a potential zoonosis. | simian parvovirus (spv) causes severe anemia in immunocompromised macaques. the closely related erythrovirus, parvovirus b19, causes anemia in susceptible humans and can be grown in human bone marrow mononuclear cells in vitro. we hypothesized that spv may infect humans and replicate in human bone marrow mononuclear cells. | 2004 | 15529252 |
| parvovirus b19 capsid protein vp2 inhibits hematopoiesis in vitro and in vivo: implications for therapeutic use. | to evaluate the capacity of parvovirus b19 capsid protein vp2 to inhibit hematopoiesis in vitro and in vivo. if effective, a vp2-derived construct may have therapeutic and prophylactic utility in diseases associated to overproduction of hematopoietic cells. | 2004 | 15539086 |
| successful treatment with cyclosporine and high-dose gamma immunoglobulin for persistent parvovirus b19 infection in a patient with refractory autoimmune hemolytic anemia. | we describe a patient with persistent pure red cell aplasia due to human parvovirus b19 (hpvb19) infection during immunosuppressive therapy for refractory autoimmune hemolytic anemia (aiha). the patient had been given corticosteroid (cs) and/or azathioprine for aiha. during the course of treatment, reticulocyte count and hemoglobin levels decreased suddenly. bone marrow aspirate showed erythroid lineage-specific aplasia with a few giant proerythroblasts, suggesting the presence of hpvb19 infecti ... | 2004 | 15540900 |
| comparison of the transcription profile of simian parvovirus with that of the human erythrovirus b19 reveals a number of unique features. | simian parvovirus (spv) is a member of the genus erythrovirus and is closely related to the human parvovirus b19. natural and experimental infection of monkeys with spv resembles b19 infection of human. we report a detailed characterization of the viral rnas and proteins generated following transfection of cloned spv into cos cells and spv infection of the human erythroid progenitor line ut-7/epo-s1. spv and b19 are 50% identical at the nucleotide level, and although their basic transcription an ... | 2004 | 15542645 |
| recurrent high level parvovirus b19/genotype 2 viremia in a renal transplant recipient analyzed by real-time pcr for simultaneous detection of genotypes 1 to 3. | organ transplant recipients infected with parvovirus b19 frequently develop persistent viremia associated with chronic anemia and pure red cell aplasia. in this study, a male renal transplant recipient who had been infected with parvovirus b19/genotype 2 after renal transplantation at the age of 34 years is described. the patient was repeatedly treated with high dose intravenous immunoglobulin (ivig) that resulted in the resolvement of symptoms but not in virus eradication. during an observation ... | 2005 | 15543575 |
| association between enterovirus endomyocardial infection and late severe cardiac events in some adult patients receiving heart transplants. | enteroviruses and other cardiotropic viruses have been associated with the development of late severe adverse cardiac events in infants receiving heart transplants. however, the source and the chronology of cardiac allograft infection by an enterovirus in patients receiving heart transplants remain unknown. using rt-pcr and immunohistochemistry assays, endomyocardial tissue samples of 30 adult patients were tested to detect the presence of specific enterovirus 5' non-coding (5'nc) sequences and ... | 2005 | 15543592 |
| [rheumatologic manifestations of parvovirus b19 infection]. | rheumatologic manifestations of parvovirus b19 infection is a recent individualization. they are rather frequent and varied and meet at the young adult's. generally, it is responsible for acute, bilateral and symmetrical arthritis, usually involving distal joints and sometimes associated to signs caused by viral infections. prolonged articular forms were discribed but are rare being able to sometimes feign a nocive rheumatoid arthritis because of their clinical aspect and of a seropositivity. ax ... | 2004 | 15552021 |
| parvovirus b19-associated transient red cell aplasia in children: the role of bone marrow examination in unusual presentations. | human parvovirus b19 (pv b19) infection in children commonly presents as fifth disease. transient red cell crisis, the other manifestation of pv b19 infection, is usually reported in children with chronic hemolytic anemia, with a worsening of the anemia. however, this condition may pass unrecognized in children without an underlying hemolytic disorder, since the anemia may be of a short duration and self-limiting. the authors report 3 cases of pv b19-induced transient aplastic in different clini ... | 2004 | 15552814 |
| successful high-titer immunoglobulin therapy for persistent parvovirus b19 infection in a lymphoma patient treated with rituximab-combined chemotherapy. | a 40-year-old female diagnosed with follicular lymphoma was treated with rituximab-combined chemotherapy. although she achieved complete remission, she developed progressive anemia and reticulocytopenia. bone marrow examination revealed features of pure red cell aplasia and hemophagocytosis. in addition, the appearance of large pronormoblasts suggested that she was infected with parvovirus b19. excess viral dna in her bone marrow confirmed that her illness was caused by persistent parvovirus b19 ... | 2004 | 15558805 |
| parvovirus b19 infection during pregnancy and development of hydrops fetalis despite the evidence for pre-existing anti-b19 antibody: how reliable are serological results? | primary infection with human parvovirus b19 during pregnancy can lead to fetal hydrops, abortion, or stillbirth. however, reinfection in the presence of pre-existing anti-b19 antibody is generally assumed to have no significant effect on the developing fetus. | 1994 | 15566756 |
| human parvovirus b19 serology with recombinant vp1 and vp2 antigens: diagnosis of acute infections by detecting b19-specific igm and iga antibodies. | the availability of immunoassays for the laboratory diagnosis of human parvovirus b19 (b19) infection, which is commonly associated with erythema infectiosum in children and arthropathy and arthralgia in adults has been hampered by the lack of native b19 antigen. the production of abundant supplies of recombinant (r) b19 proteins, through the cloning of the b19 genome into expression vectors, has led to a proliferation of assays for detecting b19-specific antibodies. | 1994 | 15566779 |
| development of an igm capture assay for the diagnosis of b19 parvovirus infection using recombinant baculoviruses expressing vp1 or vp2 antigens. | the clinical manifestations of human parvovirus b19 infection are often similar to those induced as the result of infection by other infectious agents such as rubella and some bacteria. although diagnosis of b19 infection is feasible by detection of specific antibodies, the tests require viraemic serum as a source of antigen. this inevitably leads to problems of reproducibility and dependence upon appropriate high quality clinical material. | 1995 | 15566800 |
| laboratory diagnosis of intrauterine and perinatal virus infections. | intrauterine infection with rubella, cytomegalovirus (cmv), varicella zoster virus (vzv), parvovirus b19 and human immunodeficiency virus type 1 (hiv-1) may occur following maternal infection. diagnosis of congenital infection in the neonate is dependent on the appropriate laboratory techniques being used. prenatal diagnosis of intrauterine infection may also be indicated. herpes simplex virus (hsv), hiv-1, vzv, enteroviruses, hepatitis b (hbv) and hepatitis c viruses (hcv), human t-cell lymphot ... | 1996 | 15566870 |
| igg subclass response to human parvovirus b19 infection. | igg antibodies are essential to immunity against human parvovirus b19 and can neutralize infection both in bone marrow cell cultures infected in vitro and in chronically infected immunosuppressed individuals. | 1996 | 15566889 |
| strengths and limitations of the model virus concept. | new plasma- or cell culture-based pharmaceutical manufacturing processes must be validated for their ability to eliminate potentially contaminating pathogens. to evaluate the virus elimination potential of such a process, current guidelines propose the use of model viruses. this approach is discussed based on two examples. these examples show the strengths of this approach but also its limitations. the blood processing industry was recently challenged by the emergence of a west nile virus (wnv) ... | 2004 | 15568644 |
| therapy of other viral infections: herpes to hepatitis. | over the past several years, there has been an increase in knowledge pertaining to the diagnosis and management strategies for the herpes family (types 1-8), the pox viruses, mumps, measles, rubella, and parvovirus b19 as well as the viral etiologies of hepatitis. various antiviral treatments, such as nucleoside analogs and interferon therapy, have been available to reduce the signs and symptoms of these common viral infections. this article summarizes the preferred treatment strategies to be em ... | 2004 | 15571496 |
| report of a collaborative study to calibrate the second international standard for parvovirus b19 antibody. | a collaborative study was undertaken to assess the suitability of a replacement for the first international standard for parvovirus b19 igg, human serum and to calibrate it in iu. the proposed standard, which is a pool of sera from 16 us blood donors, was assayed along with the first international standard, a coded duplicate of the proposed standard and a plasma sample from a single blood donor. nine laboratories from eight countries participated in the studies and five different assay kits were ... | 2004 | 15572102 |
| detection of parvovirus b19, cytomegalovirus and enterovirus infections in cases of intrauterine fetal death. | maternal infections with parvovirus b19, cytomegalovirus (cmv) and enterovirus have been associated with intrauterine fetal death (iufd), but the incidence of these infections is not clear. this prospective study was conducted to estimate this incidence. | 2004 | 15576274 |
| human parvovirus b19-induced acquired pure amegakaryocytic thrombocytopenia. | | 2005 | 15606559 |
| one-year results of basiliximab induction and tacrolimus associated with sequential steroid and mmf treatment in pediatric kidney transplant recipient. | we report the 1-year results with a triple immunosuppressive regimen in pediatric recipients of a first kidney transplant, in order to evaluate its safety and efficacy in the prevention of acute rejection and in the reduction of steroid side effects. the immunosuppression is as follows: (i) basiliximab (20 mg if body weight >30 kg; 10 mg if < 30 kg) is given pretransplant and at day 4; (ii) tacrolimus (tac) is administered in order to obtain blood trough levels of 10-20 and 5-10 ng/ml during and ... | 2005 | 15612981 |
| [vasculitis associated with viral infections]. | viruses, the cause of vasculitis: although the majority of systemic vasculitis are of unknown causes, the responsibility of a viral infection has been formally demonstrated in some of them and specific treatment can permanently cure them. each virus incriminated accounts for a particular type of vasculitis. hepatitis b viral infection (hbv): is the cause of polyarteritis nodosa in 36 to 50% of cases. the onset of the symptomatology is acute, usually within a few months following the infection; i ... | 2004 | 15615248 |
| is giant cell arteritis an infectious disease? biological and epidemiological evidence. | the etiology of giant cell arteritis and polymyalgia rheumatica remains unknown, although the hla-dr4 group and the pre-existence of a degenerative vascular disease are confirmed risk factors. the incidence may vary between countries, but the north-south gradient should be considered with caution because of potential detection and collection bias. infectious trigger factors have been looked for both at the epidemiological and biological level: annual, cyclic variations of incidence have been sho ... | 2004 | 15615251 |
| blood protein purification and simultaneous removal of nonenveloped viruses using tangential-flow preparative electrophoresis. | gradiflow is new technology allowing purification of important blood proteins from viral contaminated plasma. protein purification is based on unique scalable tangential-flow preparative electrophoresis, and is distinct from current technology because protein purification and virus removal are performed in the same step. this one-step removal and purification exploits both the size and charge of target proteins. the medically important blood proteins, immunoglobulin g (igg) and alpha-1-antitryps ... | 2005 | 15624167 |
| [application of factor analysis in the study of risk factors on human parvovirus b19 infection during pregnancy]. | to explore the risk factors of human parvovirus b19 infection in pregnancy and to provide guidelines for its prevention and control strategy. | 2004 | 15631741 |
| parvovirus b19 viraemia in dutch blood donors. | blood, donated by asymptomatic donors, may contain and transmit parvovirus b19. to investigate the dynamics of parvovirus viraemia in asymptomatic blood donors, we studied the amounts of parvovirus dna in pools of donor plasma, the prevalence of parvovirus antibodies among blood donors in relation to age, and the seasonal and year-to-year variation of the incidence of parvovirus infection in the netherlands. the incidence of parvovirus infection follows a seasonal cycle and a cycle of several ye ... | 2004 | 15635975 |
| the laboratory confirmation of suspected measles cases in settings of low measles transmission: conclusions from the experience in the americas. | the americas have set a goal of interrupting indigenous transmission of measles using a strategy developed by the pan american health organization (paho). this strategy includes recommendations for vaccination activities to achieve and sustain high immunity in the population and is complemented by sensitive epidemiological surveillance systems developed to monitor illnesses characterized by febrile rash, and to provide effective virological and serological surveillance. a key component in ensuri ... | 2004 | 15640921 |
| variants of b19. | the human parvovirus b19 (b19), the only known human pathogenic parvovirus, was discovered in 1975. like other parvoviruses it is a small (22-24 nm diameter) non-enveloped icosahedral virus, with a single-stranded genome. as part of its life history it replicates to high titre in the bone marrow, and in acute infection high titres of infectious virus are present in the peripheral blood. parvovirus b19 is therefore not an uncommon 'contaminant' of blood and blood products. in addition the lack of ... | 2004 | 15645675 |
| quantitative nat for pathogen inactivation verification. | a novel quantitative nucleic acid test (q-nat) technology has been developed to demonstrate, quantify and verify pathogen inactivation by methods that break pathogen nucleic acids, specifically, gamma irradiation. the q-nat technology provides significant advantages in cost, efficiency and broad applicability compared with traditional methods for pathogen inactivation detection and quantification such as cell culture. | 2004 | 15645676 |
| cell culture-based assay of parvovirus b19 and the relevance of animal model viruses. | one of the most challenging problems with the interpretation of virus validation studies is the relevance of the model viruses investigated. there are cases where model viruses have been shown to predict the inactivation/removal of a specific virus while unexpected results were obtained in other cases such as inactivation of parvovirus b19 by pasteurisation or treatment with guanidinium hydrochloride. the understanding of the mechanism underlying virus inactivation/removal might be helpful for t ... | 2004 | 15645679 |
| [the role of viral infection in emergence and progression of primary glomerulonephritis and some nephropathies]. | the results of this study confirm the importance of virus infection in nephropathy onset and progression. virus-like inclusions were found at electron microscopy in 70 (46 children and 24 adults) of 136 total diagnostic biopsies. in another group of patients (68 adults--50 males and 18 females) with virus-like inclusions in the kidney biopsy studied electron microscopically detected 12 types of viruses observed by pcr in the tissue, present either separately or in various combinations, 2 or 3 si ... | 2004 | 15648164 |
| parvovirus b19 does not bind to membrane-associated globoside in vitro. | the glycosphingolipid globoside (globotetraosylceramide, gb4cer) has been proposed to be the cellular receptor of human parvovirus b19. quantitative measurements of the binding of parvovirus b19 to gb4cer were performed to explore the molecular basis of the virus tropism. solid-phase assays with fluorescence-labeled liposomes or 125iodine-labeled empty capsids were used to characterize the specificity of binding. in addition, surface plasmon resonance on lipid layers, as well as isothermal titra ... | 2005 | 15661151 |
| seroprevalence, incidence of prenatal infections and reliability of maternal history of varicella zoster virus, cytomegalovirus, herpes simplex virus and parvovirus b19 infection in south-western finland. | to study seroprevalence and incidence and fetal transmission of varicella zoster virus (vzv), cytomegalovirus (cmv), herpes simplex virus (hsv) types 1 and 2 and parvovirus b19 infections during pregnancy and to evaluate the reliability of maternal past history of vzv, hsv and parvovirus infections. | 2005 | 15663397 |
| inactivation of parvovirus b19 by liquid heating incorporated in the manufacturing process of human intravenous immunoglobulin preparations. | several reports have suggested the possible transmission of human parvovirus b19 (b19) through the administration of plasma derivatives that had undergone virus inactivation by various types of heat treatment. however, none of the reports evaluated and discussed the inactivation of b19 by the heat treatment that is implemented in the individual manufacturing processes of such products. the present study evaluated the ability to inactivate b19 of liquid-heat treatment at 60 degrees c for 10 h tha ... | 2005 | 15667545 |
| agranulocytosis associated with parvovirus b19 infection in otherwise healthy patients. | in the course of 6 years, 23 otherwise healthy patients with acute febrile illness and leukopenia were diagnosed as having acute parvovirus b19 infection. five of these patients had agranulocytosis associated with acute parvovirus b19 infection and one had chronic agranulocytosis due to persistent parvovirus b19 infection. the diagnosis was made after positive anti-parvovirus b19 igm antibodies were found in all of the patients and viral dna was detected by pcr in four patients. neutropenia and ... | 2004 | 15668091 |
| etiology of mumps-like illnesses in children and adolescents vaccinated for measles, mumps, and rubella. | the possible viral etiology of mumps-like illnesses in patients vaccinated for measles, mumps, and rubella (mmr) was studied by use of serum samples prospectively collected, during 1983-1998, from 601 acutely ill finnish children and adolescents with mumps-like symptoms. mumps virus was excluded by testing serum samples for mumps antibodies, and the serum samples were further tested for antibodies to adenovirus, enterovirus, epstein-barr virus, parainfluenza virus types 1-3, and parvovirus b19. ... | 2005 | 15688285 |
| pediatric red cell disorders and pure red cell aplasia. | anemia in children may arise from a wide variety of pathogenetic mechanisms that include congenital and acquired disorders. often the diagnostic considerations include disorders that are not seen commonly in adults and lifelong disorders that arise in children and persist throughout life. consideration of diverse causes of anemia such as red cell membrane disorders, red cell enzymopathies, congenital dyserythropoietic anemias, congenital sideroblastic anemias, and hereditary pure red cell aplasi ... | 2004 | 15690644 |
| lupus-like presentation of parvovirus b19 infection. | to describe 2 cases of parvovirus b19 (b19) infection mimicking systemic lupus erythematosus (sle) and to identify all cases of sle imitated by and/or associated with b19 in the medical literature. | 2005 | 15692957 |
| parvovirus b19: another agent associated with remitting seronegative symmetrical synovitis with pitting edema. | a number of conditions have been associated with remitting seronegative symmetrical synovitis with pitting edema (rs3pe) and the controversy of whether this should be considered a syndrome rather than a disease continues. there are few reports on the role of infectious agents in the etiology of rs3pe, and human parvovirus b19 has not previously been linked to this syndrome. we describe a patient with rs3pe syndrome in association with positive serology and viremia for parvovirus b19. a 9-year fo ... | 2005 | 15693107 |
| high prevalence of viral genomes and multiple viral infections in the myocardium of adults with "idiopathic" left ventricular dysfunction. | for a long time, enteroviruses have been considered to be the most common cause of acute viral myocarditis (mc), with possible transition from mc to dilated cardiomyopathy (dcm). recent investigations have shown, however, that other viruses are also frequently encountered in mc patients, suggesting that persistence of various virus species may play a pathogenic role in the transition from mc to dcm. the purpose of this study was to screen endomyocardial biopsies (embs) from patients with "idiopa ... | 2005 | 15699250 |
| high prevalence of cardiac parvovirus b19 infection in patients with isolated left ventricular diastolic dysfunction. | the etiology of left ventricular (lv) isolated diastolic dysfunction often remains unclear. in the present study, we report a strong association between parvovirus b19 (pvb19) genomes and isolated lv diastolic dysfunction. | 2005 | 15710767 |
| cytomegalovirus infection of the heart is common in patients with fatal myocarditis. | although enteroviruses and adenoviruses are considered to be the leading causes of the usually mild clinical myocarditis, little is known about the etiology of severe or fatal myocarditis. | 2005 | 15714413 |
| [parvovirus b19 thrombocytopenic purpura complicated with a cerebral hemorrhage]. | severe hemorrhage complications are rare in idiopathic thrombocytopenic purpura. this pathology is often considered as benign. case report: we report the case of a four-year-old boy presenting a parvovirus b19 idiopathic thrombocytopenic purpura. despite early and repeated use of intravenous immunoglobulin, the evolution was characterized by the secondary apparition of a cerebral hemorrhage. it was lethal seven days after the initial diagnosis. conclusion: parvovirus b19 should be investigated a ... | 2005 | 15734124 |
| human parvovirus b19 vp2 empty capsids bind to human villous trophoblast cells in vitro via the globoside receptor. | pregnant women acutely infected with human parvovirus b19 (b19) may transmit the virus to the developing fetus. the mechanism whereby the virus interacts with the placenta is unknown. it is known that globoside receptor is required for successful infection of the target cells, which are the highly undifferentiated, actively dividing colony and burst-form units of the erythroid series. globoside is present on trophoblast cells which have intimate contact with maternal blood, and may therefore ser ... | 2004 | 15739820 |
| amplification of autoimmune disease by infection. | reports of infection with certain chronic persistent microbes (herpesviruses or chlamydiae) in human autoimmune diseases are consistent with the hypothesis that these microbes are reactivated in the setting of immunodeficiency and often target the site of autoimmune inflammation. new experimental animal models demonstrate the principle. a herpesvirus or chlamydia species can be used to infect mice with induced transient autoimmune diseases. this results in increased disease severity and even rel ... | 2005 | 15743493 |
| central nervous system vasculitis secondary to parvovirus b19 infection in a pediatric renal transplant patient. | central nervous system (cns) vasculitis secondary to chronic parvovirus b19 (b19) infection presenting with recurrent neurological findings is a very rare disorder during childhood. here we report a 12-year-old boy with a renal transplant who had chronic b19 infection with skin eruptions and recurrent episodes of encephalopathy with focal neurological deficits. b19 dna was detected in blood, bone marrow, and skin biopsy specimens. repeat cranial magnetic resonance (mr) imaging during each episod ... | 2005 | 15747163 |
| persistent parvovirus-associated chronic fatigue treated with high dose intravenous immunoglobulin. | we report a 16-year-old boy with no evidence of immunodeficiency who had a 2-year history of chronic fatigue, low grade fever and slapped-cheek rash associated with chronic parvovirus b19 viremia. prolonged intravenous immunoglobulin therapy resulted in resolution of his symptoms and viremia. intravenous immunoglobulin may be useful in the resolution of parvovirus viremia regardless of immune status. | 2005 | 15750469 |
| atypical presentation of parvovirus infection. | | 2005 | 15750474 |
| autologous peripheral blood progenitor cells are a potential source of parvovirus b19 infection. | parvovirus b19 is a cause of delayed red blood cell (rbc) engraftment after marrow transplantation (bmt). the diagnosis of parvovirus infection requires serologic and dna testing in the context of clinical disease and characteristic marrow morphologic findings; however, the source of infection is often difficult to determine. | 2005 | 15752157 |
| parvovirus b19 infection: fifth disease and more. | | 2005 | 15767606 |
| parvovirus b19 in an immunocompetent adult patient with acute liver failure: an underdiagnosed cause of acute non-a-e viral hepatitis. | there are occasional pediatric reports of parvovirus b19-associated transient acute hepatitis and hepatic failure. a case of a 34-year-old immunocompetent woman who developed severe and prolonged but self-limited acute hepatitis and myelosuppression following acute parvovirus b19 infection is reported. parvovirus b19 may be the causative agent in some adult cases of acute non-a-e viral hepatitis and acute liver failure. | 2005 | 15776137 |
| fatal course of parvovirus b19-associated myocarditis in a female liver transplant recipient. | acute myocarditis may result in severe hemodynamic compromise with fatal outcome. furthermore, recent studies suggest myocarditis as a major cause of sudden unexpected death. a variety of cardiotropic viral, rickettsial, and bacterial infectious agents have been identified to date. parvovirus b19 (pvb19) is usually benign in childhood, but it may also cause death due to myocarditis. we present here the case of an adult female who presented with fatigue, dyspnea on exertion, and orthostatic dizzi ... | 2005 | 15776413 |
| susceptibility to infectious rash illness in pregnant women from diverse geographical regions. | varicella-zoster (vzv), rubella (rv) and parvovirus b19 (b19v) infections are important causes of rash illness in pregnancy, due to their potential adverse impact on both mother and fetus. we determined susceptibility to these infections in pregnant women attending our hospital in 2002. age and nationality were recorded. sera were tested for vzv, rv, and b19v antibody by enzyme immunoassay. of 7,980 women screened for vzv igg, 11.3% were seronegative and therefore susceptible to infection. acros ... | 2004 | 15779804 |
| [parvovirus b19 infection revealed by prolonged dysesthesia]. | | 2005 | 15780899 |
| pure red cell aplasia associated with parvovirus b19 infection in renal transplantation: the first case report in mexico. | | 2005 | 15785387 |
| [dress syndrome to allopurinol: a case in dakar]. | we report a case of dress syndrome also called drug hypersensitivity reaction occurring a 47 years old senegalese man who has been taking allopurinol for 3 months. that drug was prescribed for peripheric arthralgias associated to a hyperuricemia. he presented a generalised pruritus, cutaneous lesions, fever and facial oedema. on the biological examens, hyperleucocytosis with hypereosinophilia and hyperlymphocytosis associated to the presence of segmented basophiles. in addition, a hepatic cytoly ... | 2004 | 15786619 |
| [parvovirus b9 infection in a renal transplant recipient. diagnosis by detection of viral genome in peripheral blood]. | parvovirus b19 can produce a picture known as pure red blood aplasia in recipients of solid organ. occasionally the viruses cause decrease of the other blood cells, and various extra-hematologic manifestations. common diagnosis is realised by bone marrow examination. the diagnostic value of the viral genome in the blood stream is not well defined. we reported the case of a male of 17 years of age, whose diagnosis was done by repeated determinations of the viral parvovirus b19 genome in periphera ... | 2005 | 15789539 |
| structure of adeno-associated virus type 4. | adeno-associated virus (aav) is a member of the parvoviridae, belonging to the dependovirus genus. currently, several distinct isolates of aav are in development for use in human gene therapy applications due to their ability to transduce different target cells. the need to manipulate aav capsids for specific tissue delivery has generated interest in understanding their capsid structures. the structure of aav type 4 (aav4), one of the most antigenically distinct serotypes, was determined to 13-a ... | 2005 | 15795290 |
| high frequency of parvovirus b19 dna in bone marrow samples from rheumatic patients. | human parvovirus b19 (b19) polymerase chain reaction (pcr) is now a routine analysis and serves as a diagnostic marker as well as a complement or alternative to b19 serology. the clinical significance of a positive b19 dna finding is however dependent on the type of tissue or body fluid analysed and of the immune status of the patient. | 2005 | 15797368 |
| seroprevalence of parvovirus b19 infection in daycare educators. | this study was undertaken to provide first-time estimates for the seroprevalence of parvovirus b19 infection among daycare educators in montréal, canada, and to identify factors associated with seropositivity. a cross-sectional design was used. directors and educators from 81 daycare centres (dccs) were surveyed about dcc and personal characteristics respectively, and serum samples from 477 female educators were tested for parvovirus b19 igg antibodies. the seroprevalence of parvovirus b19 was 7 ... | 2005 | 15816155 |
| inactivation of viruses in platelet concentrates by photochemical treatment with amotosalen and long-wavelength ultraviolet light. | viral contamination of platelet (plt) concentrates can result in transfusion-transmitted diseases. a photochemical treatment (pct) process with amotosalen-hcl and long-wavelength ultraviolet light (uva), which cross-links nucleic acids, was developed to inactivate viruses and other pathogens in plt concentrates. | 2005 | 15819680 |
| detection of viral deoxyribonucleic acid in amniotic fluid: association with fetal malformation and pregnancy abnormalities. | to first test the hypothesis that the presence of viral nucleic acid in amniotic fluid (af) is associated with an abnormal pregnancy outcome, and second, to determine if the overall rate of polymerase chain reaction (pcr) positivity and the distribution of virus types vary geographically. | 2005 | 15824499 |
| eye anomalies: seasonal variation and maternal viral infections. | eye anomalies, including anophthalmos and microphthalmos (absent eye and small eye), are common features in congenital infection syndromes such as rubella. influenza, coxsackie virus, and parvovirus are less well-established teratogens. we report an ecologic analysis of anophthalmia/microphthalmia prevalence in england in relation to temporal variation in these infections using routine infection data. | 2005 | 15824546 |
| what is antibody-mediated pure red cell aplasia (prca)? | antibody (ab)-mediated pure red cell aplasia (prca) is an immunological pathology associated with the production of neutralizing abs that inhibit the erythropoietic activity of endogenous erythropoietin (epo) and recombinant erythropoiesis-stimulating agents (esas). although this disorder occurs very rarely, the number of reported cases has increased dramatically in recent years, predominantly in patients with chronic kidney disease (ckd)-associated anaemia receiving subcutaneous (s.c.) injectio ... | 2005 | 15827056 |